scholarly journals Centella asiatica increases B-cell lymphoma 2 expression in rat prefrontal cortex

2016 ◽  
Vol 34 (1) ◽  
pp. 10
Author(s):  
Kuswati Kuswati ◽  
Djoko Prakosa ◽  
Brian Wasita ◽  
Nanang Wiyono
Keyword(s):  
Prefrontal Cortex ◽  
B Cell ◽  
Restraint Stress ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
B Cell Lymphoma ◽  
Control Group ◽  
Sprague Dawley ◽  
Stress Group ◽  
Sprague Dawley Rats

<p>BACKGROUND<br />Stress is one of the factors that cause apoptosis in neuronal cells. Centella<br />asiatica has a neuroprotective effect that can inhibit apoptosis. This study<br />aimed to examine the effect of Centella asiatica ethanol extract on B-cell<br />lymphoma 2 (Bcl-2) protein expression in the prefrontal cortex of rats.</p><p>METHODS<br />An experimental study was conducted on 34 brain tissue samples from male<br />Sprague Dawley rats exposed to chronic restraint stress for 21 days. The<br />samples were taken from following groups: non-stress group K, negative<br />control group P1 (stress + arabic gum powder), P2 (stress + C.asiatica at<br />150 mg/kgBW), P3 (stress + C.asiatica at 300 mg/kg BW), P4 (stress +<br />C.asiatica at 600 mg/kg body weight) and positive control group P5 (stress<br />+ fluoxetine at 10 mg/kgBW). The samples were made into sections that<br />were stained immunohistochemically using Bcl-2 antibody to determine the<br />percentage of cells expressing Bcl-2. Data were analyzed using one way<br />ANOVA test followed by a post - hoc test.</p><p>RESULTS<br />There were significant differences in mean Bcl-2 expression between the<br />groups receiving Centella asiatica compared with the non-stress group and<br />stress-only group (negative control group) (p&lt;0.05). The results were<br />comparable to those of the fluoxetine treatment group.</p><p>CONCLUSION</p><p>The Centella asiatica ethanol extract was able to increase Bcl-2 expression<br />in the prefrontal cortex of Sprague Dawley rats exposed to restraint stress.<br />This study suggests that Centella asiatica may be useful in the treatment of<br />cerebral stress.</p>

scholarly journals The effect of ethanol extract of pegagan (Centella asiatica) on the performance of Wistar rats after restraint stress

2010 ◽  
Vol 8 (1) ◽  
pp. 11-16
Author(s):  
BAARID LUQMAN HAMIDI ◽  
SAMIGUN SAMIGUN ◽  
ANIK LESTARI
Keyword(s):  
Restraint Stress ◽  
Wistar Rats ◽  
Radial Maze ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
The Body ◽  
Control Group ◽  
Error Type ◽  
Stress Group ◽  
Significant Difference

Hamidi BL, Samigun, Lestari A. 2010. The effect of extract ethanol of pegagan (Centella asiatica) on the performance of Wistar rats after restraint stress. Biofarmasi 8: 11-16. The aim of this research was to investigate the effects of extract ethanol of pegagan (Centella asiatica) after treated with restraint stress by measuring the eight arms radial maze performance of rats. Pre-test and post-test controlled groups design was applied in this research. Male Wistar rats (Rattus novergicus) with the mean age of 8 weeks and the body weight of 150-200 grams which used for sample were divided randomly into 4 groups, each group consisted of 6 rats, i.e. (i) control group (without any treatment), (ii) stress group (it was given by restraint stress for 2 hours/day for each rat), (iii) pegagan group (it was given by 0.3 mg/g BW/day/rat extract ethanol of pegagan), and (iv) pegagan and stress group (it was given by 0.3 mg/g BW/day/rat extract ethanol of pegagan and restraint stress for 2 hours/day for each rat). The treatments were given for 21 days. Within 12 days for each pre-treatment and post-treatment, a test on the eight arm radial maze was conducted on individual rat to observe its performance. The assessment of rat performance in the eight arms radial maze test was conducted based on error type B. Kruskal-Wallis and Mann-Whitney tests with SPSS for Windows 16 version were applied to analyze statistically the difference between four groups. Kruskal-Wallis test was used to show the significant performance level difference between four groups of rats with p=0.001, while Mann-Whitney test was used to determine the significant difference between stress group and pegagan group (p=0.001), also stress group and pegagan and stress group (p=0.001). The result of research showed that there was no significant difference between control group and stress group (p=0.051), control group and pegagan group (p=0.143), control group and pegagan and stress group (p=0.143), also pegagan group and pegagan and stress group (p=0.952). It was concluded that extract ethanol of pegagan improved the performances of rats on the eight arms radial maze after treated with restraint stress.

scholarly journals THE COMBINATION INFUSION OF Talinum paniculatum (Jacq.) Gaertn., Centella asiatica (L.) Urb. AND Curcuma xanthorrhiza Roxb. EFFECTS ON TESTOSTERONE LEVELS AND INTRODUCTION, CLIMBING AND COITUS FREQUENCIES IN MALE SPRAGUE-DAWLEY RATS

2019 ◽  
Vol 12 (1) ◽  
pp. 33-40
Author(s):  
Nuning Rahmawati ◽  
Ika Yanti Marfuatush Sholikhah
Keyword(s):  
Treatment Group ◽  
Antibacterial Effect ◽  
Centella Asiatica ◽  
Control Group ◽  
Sprague Dawley ◽  
Pharmacological Activities ◽  
Testosterone Levels ◽  
Sd Rats ◽  
Sprague Dawley Rats ◽  
Curcuma Xanthorrhiza

ABSTRACT Talinum paniculatum (som Java) reported to have many pharmacological activities such as increasing spermatozoa quality, cell regenerations, fertility, and antibacterial effect both empirically and scientifically. This study aimed to prove the combination infusion effects of som Java, gotu kola, and temulawak on introduction, climbing, and coitus levels as well as testosterone levels in male Sprague Dawley (SD) rats. SD rats were divided into 2 groups, control group received aquadest, treatment group received formula a mixture of som java Talinum paniculatum) root, gotu kola (Centella asiatica) herb, and Javanese turmeric (Curcuma xanthorrhiza) rhizome with the dose of 491,2 mg/200 g BW for 7 consecutive days. Artificial estrus of female SD rats were induced by administering estradiol valerat 2 mg/200 g BW 48 hours before observation. The determined parameters were levels of testosterone as well as introduction, climbing and coitus frequencies. Administration of estradiol valerat led to artificial estrus induction of female SD rats. The administration of the combination infusion dose 491.2 mg/200 g BW significantly increased testosterone level as well as introduction and climbing frequencies of male SD rats as of 27.77%; 86.39% and 69.17% respectively. ABSTRAK Som jawa (Talinum paniculatum) secara empiris dan ilmiah terbukti telah banyak dimanfaatkan oleh masyarakat untuk mengatasi berbagai masalah kesehatan diantaranya untuk meningkatkan kualitas dan kuantitas spermatozoa, regenerasi sel, fertilitas, dan sebagai antibakteri. Penelitian ini dilakukan untuk membuktikan efek peningkatan kadar hormon testosteron serta frekuensi introduction, climbing, dan coitus tikus jantan SD dari pengaruh infusa kombinasi som jawa, pegagan, dan temulawak. Hewan uji tikus galur SD dibagi menjadi 2 kelompok, yaitu kelompok kontrol diberikan akuades, sedangkan kelompok perlakuan diberikan infusa ramuan akar som jawa, herba pegagan, dan rimpang temulawak dosis 491,2 mg/200 g bb selama 7 hari berturut-turut. Induksi estrus artifisial tikus betina dengan pemberian estradiol valerat 2 mg/200 g bb dilakukan 48 jam sebelum pengamatan. Parameter yang diukur meliputi kadar testosterone, frekuensi introduction, climbing, dan coitus. Dibandingkan dengan kelompok kontrol, pemberian infusa ramuan tidak berefek pada frekunsi coitus, namun mampu meningkatkan kadar testosteron serta frekuensi introduction dan climbing tikus jantan sebesar 27,77; 86,39 dan 69,17%.          

scholarly journals Psidium guajava leaves decrease arthritic symptoms in adjuvant-induced arthritic rats

2016 ◽  
Vol 34 (3) ◽  
pp. 197
Author(s):  
Hanif Nasiatul Baroroh ◽  
Esti Dyah Utami ◽  
Anisyah Achmad
Keyword(s):  
Knee Joint ◽  
Histopathological Examination ◽  
Chronic Phase ◽  
Ethanol Extract ◽  
Psidium Guajava ◽  
Control Group ◽  
Sprague Dawley ◽  
Group Iii ◽  
Group I ◽  
Sprague Dawley Rats

BACKGROUND <br />Guava is an herbal with proven antioxidant and anti-inflammatory properties. The aim of this study was to investigate the anti-arthritic activity of the ethanol extract of Psidium gujava leaves (EEPG) against complete Freund’s adjuvant (CFA) induced arthritis in rats. <br /><br />METHODS<br />An experimental study was conducted on 40 male Wistar Sprague Dawley rats, which were divided into 5 groups. Each group was induced with 0.2 mL CFA (1 mg/mL) on day 1 and 0.1 CFA mL booster injection on day 5. Group I served as an arthritic control, group II received dexamethasone (6.75 mg.kg-1 orally), group III, IV and V received EEPG at oral doses of 250, 500, and 750 mg/kg BW, respectively, on days 14 to 28. Anti-arthritic activity was observed from the arthritis score, the paw circumference was measured on days 0, 1, 4, 8, 12, 16, 20, 24, and 28, the mobility score was determined on days 12 and 28, and the histolopathology of the knee joint was examined on day 29. <br /><br />RESULTS<br />Ethanol extract of Psidium guajava leaves significantly suppressed the swelling of the paws in chronic phase based on increasing of edema (%), while starting on day 20. EEPG at 250 mg/kg was most effective in significantly reducing arthritis scores (p&lt;0.05). Histopathological examination showed repair of the knee joint synovial membrane and cartilage.<br /> <br />CONCLUSIONS<br />Psidium guajava leaf extract is effective in decreasing the inflammatory response and arthritic symptoms in rats with adjuvant-induced arthritis. Psidium guajava leaves can be developed into an alternative anti-arthritis treatment.

scholarly journals Propolis increases neuronal count in hippocampal area CA1 and prefrontal cortex in stressed rats

2017 ◽  
Vol 36 (3) ◽  
pp. 214
Author(s):  
Kuswati Nugroho ◽  
Ety Sari Handayani ◽  
Zainuri Sabta Nugraha
Keyword(s):  
Prefrontal Cortex ◽  
Cell Count ◽  
Neuroprotective Effect ◽  
Neuronal Cell ◽  
Sprague Dawley ◽  
Stress Group ◽  
Induced Stress ◽  
Area Ca1 ◽  
Sprague Dawley Rats ◽  
Hippocampal Area

Background <br />Stress induces neuronal cell damage in the hippocampus and prefrontal cortex. Propolis has a neuroprotective effect that can inhibit apoptosis and decrease neuronal cell count. This study aimed to determine the effect of propolis on neuronal cell count in hippocampal area CA1 and prefrontal cortex in Sprague Dawley rats with induced stress.<br /><br />Methods<br />A study of laboratory experimental design was conducted involving 24 male Sprague-Dawley Rattus norvegicus. The animals were randomly divided into 4 groups, i.e. controls (K), and stress groups P1, P2 and P3. Controls did not receive treatment, stress group (P1) received stress treatment, groups P2 and P3 received stress and propolis at 100 and 200 mg/kgBW, respectively. Stress and propolis were given for 14 days, followed by termination. The number of neurons in the hippocampal area CA1 and prefrontal cortex were counted. One way ANOVA was used to analyze the data.<br /><br />Results <br />The neuronal count in the hippocampal area CA1 and prefrontal cortex in the stress group (P1) was lower than in groups K, P2 and P3. There were significant differences in the neuronal count of the hippocampal area CA1 between P1 and P3 and P1 and K (p=0.019) and also in the neuronal count of the prefrontal cortex between P1 and P2, P3 and K (p=0.002).<br /><br />Conclusions <br />This study strongly suggest that propolis inhibits the decrease in neuronal count in in the hippocampal area CA1 and prefrontal cortex of Sprague Dawley rats with induced stress. The present study suggests a potential neuroprotective effect of propolis in the prevention of neurodegenerative disorders.

scholarly journals Expression of Autophagy-Related Factors LC3A and Beclin 1 and Apoptosis-Related Factors Bcl-2 and BAX in Osteoblasts Treated With Sodium Fluoride

2021 ◽  
Vol 12 ◽  
Author(s):  
Lin Xu ◽  
Chaonan Deng ◽  
Ying Zhang ◽  
Lina Zhao ◽  
Yan Linghu ◽  
...  
Keyword(s):  
Sodium Fluoride ◽  
Quantitative Polymerase Chain Reaction ◽  
B Cell Lymphoma ◽  
Treated Group ◽  
Beclin 1 ◽  
Control Group ◽  
Sprague Dawley ◽  
Related Factors ◽  
Sprague Dawley Rats ◽  
Protein Expressions

ObjectiveThis study aims to analyze the expressions of autophagy-related factors light chain 3 alpha (LC3A) and Beclin 1 and apoptosis-related factors B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X (BAX) in primary osteoblasts treated with sodium fluoride (NaF).MethodsOsteoblasts were extracted from Sprague-Dawley rats and treated with 0, 2.5, 5, and 10 mg/L NaF solutions, followed by 10 mmol/L 3-methyladenine (3-MA) for 24 h. The apoptotic rate was determined by flow cytometry, and the expressions of the autophagy- and apoptosis-related factors were measured by western blotting and real-time quantitative polymerase chain reaction.ResultsThe mRNA expressions of LC3A, Beclin 1, and BAX in the NaF-treated osteoblast group were higher than those in the control group, while the protein expressions of these factors in the NaF-treated group were significantly higher than those in the control group. However, the Bcl-2 protein expression in the NaF-treated osteoblasts was significantly decreased compared to that in the control cells. After the 3-MA treatment, the protein expressions of LC3A, Beclin 1, and Bcl-2 were significantly decreased compared with those of the NaF-treated group, whereas the expression of BAX increased. Moreover, the apoptosis rate was increased after the addition of the 3-MA inhibitor.ConclusionNaF stimulation promoted autophagy and apoptosis of the osteoblasts, suggesting the involvement of fluoride damage in these processes.

scholarly journals The Effect of Myrmecodia pendans Ethanol Extract on Inflamed Pulp: Study on Sprague Dawley Rats

2019 ◽  
Vol 3 (2) ◽  
pp. 115
Author(s):  
Janti Sudiono ◽  
Meylisa Hardina
Keyword(s):  
Treatment Group ◽  
Healing Process ◽  
Ethanol Extract ◽  
Flavonoid Compound ◽  
Control Group ◽  
Sprague Dawley ◽  
Treatment Groups ◽  
Group I ◽  
Sprague Dawley Rats ◽  
Group Ii

Background: Inflammation is a body response caused by injury and infection. Pulpitis is a pulp tissue inflammation which is the continuous process of pulp hyperemia by bacteria invasion. Myrmecodia pendans or Sarang semut is known to contain flavonoid compound which has the anti inflammation effect. The purpose of this study is to investigate the effect of Myrmecodia pendans ethanol extract on the healing process of pulp inflammation.Materials and Methods: This experimental study involved pre- and post-in vivo treatment of 27 Sprague Dawley rats in which the inducted pulpitis model was obtained by injecting 0.01 mL Porphyromonas gingivalis into the dental pulp for 48 hours. Subjects were divided randomly into Group I (negative control), Group II (pulpitis treated by Myrmecodia pendans extract ethanol as treatment group), and Group III (pulpitis treated by Ca(OH)2 as positive control group). Group II and III as pulpitis treatment groups were divided into subgroups based on the induction periods of 48 hours (2 days), 168 hours (7 days), and 366 hours (14 days). All specimens were processed into the slides and evaluated microscopically for the healing process.Results: The result of this study showed significant difference (p<0.05) among groups on day 2, 4 and 7. On day 4, the pulpitis treatment group of Myrmecodia pendans extract showed better healing process than Ca(OH)2. On day 7, the pulpitis treatment group of Ca(OH)2 showed better healing process than Myrmecodia pendans extract. On day 14, both of the pulpitis treatment groups showed normal pulp.Conclusion: Myrmecodia pendans ethanol extract is effective for the healing process of inflamed pulp.Keywords: inflamed pulp, Myrmecodia pendans, sarang semut, Ca(OH)2 , healing process

scholarly journals Acute Tocixity of Sterculia quadrifida R.Br Bark Ethanol Extract on Sprague-Dawley Rats

2018 ◽  
Vol 23 (2) ◽  
pp. 127
Author(s):  
Siswadi Siswadi ◽  
Grace S. Saragih
Keyword(s):  
Body Weight ◽  
Clinical Symptoms ◽  
Ethanol Extract ◽  
Control Group ◽  
Sprague Dawley ◽  
Weight Group ◽  
Control Group Design ◽  
White Rats ◽  
Sprague Dawley Rats ◽  
Group 5

Faloak (Sterculia quadrifida R.Br) is a medicinal plant used by people in the province of East Nusa Tenggara to treat hepatitis. This study aims to determine the acute toxicity of faloak (Sterculia quadrifida R. Br) bark ethanol extract in Sprague-Dawley (SD) male white rats. The method used is experimental using post-test only control group design. In this study the parameters used are body weight, clinical symptoms, clinical biochemistry (ALT & AST), histopathology of liver organ and mortality in rats. Twenty male Sprague-Dawley rats were randomly assigned to 5 groups, group 1 as control, group 2 was administered orally faloak bark ethanol extract at dose of 40 mg / kg body weight, group 3 at dose of 200 mg / kg body weight, group 4 at dose of 1000 mg / kg body weight and group 5 at dose of 5000 mg / kg body weight. Results of observation for 24 hours there are no dead rats, so the value of LD50 extract ethanol faloak is > 5,000 mg / kg body weight. Observations were conducted for 14 days to observed delayed occurrence of toxic effects. Oral administration of the faloak bark ethanol extract reduced the level of AST and ALT. The histopathology observation of rats liver cells showed liver cell necrosis at treatment doses of 200 - 5,000 mg / kg body weight.

scholarly journals Skeletal Muscle Metabolomic Responses to Endurance and Resistance Training in Rats under Chronic Unpredictable Mild Stress

2021 ◽  
Vol 18 (4) ◽  
pp. 1645
Author(s):  
Xiangyu Liu ◽  
Xiong Xue ◽  
Junsheng Tian ◽  
Xuemei Qin ◽  
Shi Zhou ◽  
...  
Keyword(s):  
Resistance Exercise ◽  
Endurance Exercise ◽  
Field Tests ◽  
Treadmill Running ◽  
Control Group ◽  
Mild Stress ◽  
Chronic Unpredictable Mild Stress ◽  
Sprague Dawley ◽  
Exercise Interventions ◽  
Sprague Dawley Rats

The objectives of this study were to compare the antidepressant effects between endurance and resistance exercise for optimizing interventions and examine the metabolomic changes in different types of skeletal muscles in response to the exercise, using a rat model of chronic unpredictable mild stress (CUMS)-induced depression. There were 32 male Sprague-Dawley rats randomly divided into a control group (C) and 3 experimental groups: CUMS control (D), endurance exercise (E), and resistance exercise (R). Group E underwent 30 min treadmill running, and group R performed 8 rounds of ladder climbing, 5 sessions per week for 4 weeks. Body weight, sucrose preference, and open field tests were performed pre and post the intervention period for changes in depressant symptoms, and the gastrocnemius and soleus muscles were sampled after the intervention for metabolomic analysis using the 1H-NMR technique. The results showed that both types of exercise effectively improved the depression-like symptoms, and the endurance exercise appeared to have a better effect. The levels of 10 metabolites from the gastrocnemius and 13 metabolites from the soleus of group D were found to be significantly different from that of group C, and both types of exercise had a callback effect on these metabolites, indicating that a number of metabolic pathways were involved in the depression and responded to the exercise interventions.

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