scholarly journals Analysis of the Bacterial and Fungal Community Profiles in Bulk Soil and Rhizospheres of Three Mungbean [Vigna radiata (L.) R. Wilczek] Genotypes through PCR-DGGE

2020 ◽  
Vol 77 ◽  
pp. 1-26
Author(s):  
Anna Mae M. de los Reyes ◽  
Eureka Teresa M. Ocampo ◽  
Ma. Carmina C. Manuel ◽  
Bernadette C. Mendoza
Keyword(s):  
Plant Growth ◽  
Crop Production ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Diversity Index ◽  
Agricultural Practices ◽  
Fungal Communities ◽  
Bulk Soil ◽  
Initial Assessment ◽  
Rhizosphere Effect ◽  
Soil Microbial Diversity

Each plant species is regarded to substantially influence and thus, select for specific rhizosphere microbial populations. This is considered in the exploitation of soil microbial diversity associated with important crops, which has been of interest in modern agricultural practices for sustainable productivity. This study used PCR-DGGE (polymerase chain reaction - denaturing gradient gel electrophoresis) in order to obtain an initial assessment of the bacterial and fungal communities associated in bulk soil and rhizospheres of different mungbean genotypes under natural field conditions. Integrated use of multivariate analysis and diversity index showed plant growth stage as the primary driver of community shifts in both microbial groups while rhizosphere effect was found to be less discrete in fungal communities. On the other hand, genotype effect was not discerned but not inferred to be absent due to possible lack of manifestations of differences among genotypes based on tolerance to drought under non-stressed environment, and due to detection limits of DGGE. Sequence analysis of prominent members further revealed that Bacillus and Arthrobacter species were dominant in bacterial communities whereas members of Ascomycota and Basidiomycota were common in fungal communities of mungbean. Overall, fungal communities had higher estimated diversity and composition heterogeneity, and were more dynamic under plant growth influence, rhizosphere effect and natural environmental conditions during mungbean growth in upland field. These primary evaluations are prerequisite to understanding the interactions between plant and rhizosphere microorganisms with the intention of employing their potential use for sustainable crop production.

scholarly journals Potential for Plant Growth Promotion of Rhizobacteria Associated withSalicorniaGrowing in Tunisian Hypersaline Soils

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Francesca Mapelli ◽  
Ramona Marasco ◽  
Eleonora Rolli ◽  
Marta Barbato ◽  
Hanene Cherif ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Crop Production ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Growth Promotion ◽  
Arid Ecosystems ◽  
Acetic Acid Production ◽  
Halotolerant Bacteria ◽  
Microbiome Composition

Soil salinity and drought are among the environmental stresses that most severely affect plant growth and production around the world. In this study the rhizospheres ofSalicorniaplants and bulk soils were collected fromSebkhetandChotthypersaline ecosystems in Tunisia. Depiction of bacterial microbiome composition by Denaturing Gradient Gel Electrophoresis unveiled the occurrence of a high bacterial diversity associated withSalicorniaroot system. A large collection of 475 halophilic and halotolerant bacteria was established fromSalicorniarhizosphere and the surrounding bulk soil, and the bacteria were characterized for the resistance to temperature, osmotic and saline stresses, and plant growth promotion (PGP) features. TwentyHalomonasstrains showed resistance to a wide set of abiotic stresses and were able to perform different PGP activitiesin vitroat 5% NaCl, including ammonia and indole-3-acetic acid production, phosphate solubilisation, and potential nitrogen fixation. By using agfp-labelled strain it was possible to demonstrate thatHalomonasis capable of successfully colonisingSalicorniaroots in the laboratory conditions. Our results indicated that the culturable halophilic/halotolerant bacteria inhabiting salty and arid ecosystems have a potential to contribute to promoting plant growth under the harsh salinity and drought conditions. These halophilic/halotolerant strains could be exploited in biofertilizer formulates to sustain crop production in degraded and arid lands.

scholarly journals Mixed-Cropping Between Field Pea Varieties Alters Root Bacterial and Fungal Communities

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Anthony Horner ◽  
Samuel S. Browett ◽  
Rachael E. Antwis
Keyword(s):  
Microbial Communities ◽  
Bacterial Communities ◽  
Crop Production ◽  
Agricultural Soils ◽  
Critical Role ◽  
Agricultural Practices ◽  
Microbial Interactions ◽  
Field Pea ◽  
Fungal Communities ◽  
Mixed Cropping

AbstractModern agricultural practices have vastly increased crop production but negatively affected soil health. As such, there is a call to develop sustainable, ecologically-viable approaches to food production. Mixed-cropping of plant varieties can increase yields, although impacts on plant-associated microbial communities are unclear, despite their critical role in plant health and broader ecosystem function. We investigated how mixed-cropping between two field pea (Pisum sativum L.) varieties (Winfreda and Ambassador) influenced root-associated microbial communities and yield. The two varieties supported significantly different fungal and bacterial communities when grown as mono-crops. Mixed-cropping caused changes in microbial communities but with differences between varieties. Root bacterial communities of Winfreda remained stable in response to mixed-cropping, whereas those of Ambassador became more similar to Winfreda. Conversely, root fungal communities of Ambassador remained stable under mixed-cropping, and those of Winfreda shifted towards the composition of Ambassador. Microbial co-occurrence networks of both varieties were stronger and larger under mixed-cropping, which may improve stability and resilience in agricultural soils. Both varieties produced slightly higher yields under mixed-cropping, although overall Ambassador plants produced higher yields than Winfreda plants. Our results suggest that variety diversification may increase yield and promote microbial interactions.

scholarly journals Bio-Green Foliar Spray Enhances Rice Growth and Productivity in Cambodia

2021 ◽  
Vol 4 (1) ◽  
pp. 1-7
Author(s):  
Tak Tha ◽  
Ply Preap ◽  
Seyha Sorl ◽  
Pao Srean ◽  
Visalsok Touch
Keyword(s):  
Plant Growth ◽  
Grain Yield ◽  
Crop Production ◽  
Production Systems ◽  
Rice Variety ◽  
Foliar Spray ◽  
Ammonium Phosphate ◽  
Agricultural Practices ◽  
Cost Effective ◽  
Growing Period

The use of bioproducts as biostimulants to stimulate plant growth and to increase yields as an alternative to chemical fertilizers are currently being promoted for cost-effective, sustainable and environmentally friendly agricultural practices of crop production systems. The objective of the study was to determine plant growth and productivity of rice responded to Bio Green application. A short growing period (90 – 95 days) OM-5451 rice variety was used in this study. The rice plants were cultivated in the randomized-completed block with two treatments and six replications in the plot of 2 m * 2 m.  Di-ammonium phosphate (DAP) fertilizer was applied once at a rate of 100 kg/ha. For treatment, Bio-green with a solution of 1% (v/v) was weekly applied as foliage spray; and without Bio-Green as control. The results showed the grain yield was 3.7 t/ha in the treatment and 2.83 t/ha in the control, indicating that 36.4% of the grain yield was increased. The Bio-Green could be significantly used as plant biostimulants to promote plant growth and grain yield in rice in Cambodia.

scholarly journals The multifaceted plant-beneficial rhizobacteria toward agricultural sustainability

2021 ◽  
Vol 57 (No. 2) ◽  
pp. 95-111
Author(s):  
Olubukola Babalola ◽  
Oluwaseun Adeyinka Fasusi
Keyword(s):  
Plant Growth ◽  
Soil Fertility ◽  
Crop Production ◽  
Agricultural Practices ◽  
Modern Method ◽  
Growth And Yield ◽  
Agricultural Sustainability ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting

Agricultural practices depend mainly on the use of chemical fertilisers, pesticides, and herbicides which have caused serious health hazards and have also contributed to the pollution of the environment at large. The application of plant-beneficial rhizobacteria in agrarian practices has become paramount in increasing soil fertility, promoting plant growth, ensuring food safety, and increasing crop production to ensure sustainable agriculture. Beneficial rhizobacteria are soil microorganisms that are eco-friendly and serve as a modern method of improving the plant yield, protecting the plant and soil fertility that pose no harm to humans and the environment. This eco-friendly approach requires the application of beneficial rhizobacteria with plant growth-promoting traits that can improve the nutrient uptake, enhance the resistance of plants to abiotic and biotic stress, protect plants against pathogenic microorganisms and promote plant growth and yield. This review article has highlighted the multitasking roles that beneficial rhizobacteria employ in promoting plant growth, food production, bioremediation, providing defence to plants, and maintaining soil fertility. The knowledge acquired from this review will help in understanding the bases and importance of plant-beneficial rhizobacteria in ensuring agricultural sustainability and as an alternative to the use of agrochemicals.

scholarly journals Plant Growth Promoting Rhizobacterial Biofertilizers for Sustainable Crop Production: The Past, Present, and Future

2020 ◽  
Author(s):  
Becky N. Aloo ◽  
Billy A. Makumba ◽  
Ernest R. Mbega
Keyword(s):  
Plant Growth ◽  
Crop Production ◽  
Crop Yields ◽  
Agricultural Practices ◽  
Plant Growth Promoting Rhizobacteria ◽  
Full Potential ◽  
Sustainable Crop Production ◽  
Indigenous Plant ◽  
Plant Growth Promoting ◽  
Growth Promoting

The world’s population is increasing and so are agricultural activities to match the growing demand for food. Conventional agricultural practices generally employ artificial fertilizers to increase crop yields, but these have multiple environmental and human health effects. For decades, environmentalists and sustainability researchers have focused on alternative crop fertilization mechanisms to address these challenges, and biofertilizers have constantly been researched, recommended, and even successfully-adopted for several crops. Biofertilizers are microbial formulations made of indigenous plant growth-promoting rhizobacteria (PGPR) which can naturally improve plant growth either directly or indirectly, through the production of phytohormones, solubilization of soil nutrients, and production of iron-binding metabolites; siderophores. Biofertilizers, therefore, hold immense potential as tools for sustainable crop production especially in the wake of climate change and global warming. Despite the mounting interest in this technology, their full potential has not yet been realized. This review updates our understanding of the PGPR biofertilizers and sustainable crop production. It evaluates the history of these microbial products, assesses their present state of utilization, and also critically propounds on their future prospects for sustainable crop production. Such information is desirable to fully evaluate their potential and can ultimately pave the way for their increased adoption for crop production.

scholarly journals Effects of Lentil Genotype on the Colonization of Beneficial Trichoderma Species and Biocontrol of Aphanomyces Root Rot

2020 ◽  
Vol 8 (9) ◽  
pp. 1290
Author(s):  
Navid Bazghaleh ◽  
Pratibha Prashar ◽  
Sheridan Woo ◽  
Albert Vandenberg
Keyword(s):  
Plant Growth ◽  
Root Rot ◽  
Crop Production ◽  
Growth Promotion ◽  
Plant Root ◽  
Fungal Communities ◽  
Shoot Biomass ◽  
Aphanomyces Euteiches ◽  
Trichoderma Species

Trichoderma species are opportunistic plant symbionts that are common in the root and rhizosphere ecosystems. Many Trichoderma species may enhance plant growth, nutrient acquisition, and disease resistance, and for these reasons, they are widely used in agriculture as biofertilizers or biocontrol agents. Host plant genotype and other microorganisms, such as root pathogens, may influence the efficacy of Trichoderma inoculants. Aphanomyces euteiches is an important soil-borne oomycete in western Canada that causes root rot in legume crops such as lentil and pea, and there is not yet any significantly resistant varieties or effective treatments available to control the disease. In this study, the composition of root-associated fungal communities and the abundance of Trichoderma species, T. harzianum strain T-22 and T. virens strain G41, was determined in the roots of eight Lens genotypes based on internal transcribed spacer (ITS) Illumina MiSeq paired-end sequencing, both in the presence and the absence of the root rot pathogen Aphanomyces euteiches. Biocontrol effects of T. harzianum on A. euteiches was also examined. Significant genotypic variations were observed in the composition of root-associated fungal communities and the abundance of the different Trichoderma species in the lentil roots. The presence of A. euteiches altered the composition of Trichoderma found associated to the lentil genotypes. Biocontrol of A. euteiches by T. harzianum T22 species was observed in vitro and positive correlations between the abundance of Trichoderma and plant root and shoot biomass were observed in vivo. These findings revealed that lentil genotype and infection by the phytopathogen A. euteiches greatly influenced the colonization of root-associated fungi and the abundance of the Trichoderma species, as well as the effect on plant growth promotion. The multipartite interactions observed among lentil genotypes, Trichoderma species and A. euteiches suggest possibilities to select compatible host-beneficial microbe combinations in lentil breeding programs and to develop application strategies to harness the beneficial effects of Trichoderma inoculants in sustainable crop production systems.

scholarly journals Fish By-Product Use as Biostimulants: An Overview of the Current State of the Art, Including Relevant Legislation and Regulations within the EU and USA

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1122 ◽  
Author(s):  
Moses Madende ◽  
Maria Hayes
Keyword(s):  
Plant Growth ◽  
Crop Production ◽  
Crop Yields ◽  
Production Systems ◽  
Genetic Selection ◽  
Agricultural Practices ◽  
Cost Effective ◽  
Protein Hydrolysates ◽  
Negative Effects ◽  
Waste Products

Crop production systems have adopted cost-effective, sustainable and environmentally friendly agricultural practices to improve crop yields and the quality of food derived from plants. Approaches such as genetic selection and the creation of varieties displaying favorable traits such as disease and drought resistance have been used in the past and continue to be used. However, the use of biostimulants to promote plant growth has increasingly gained attention, and the market size for biostimulants is estimated to reach USD 4.14 billion by 2025. Plant biostimulants are products obtained from different inorganic or organic substances and microorganisms that can improve plant growth and productivity and abate the negative effects of abiotic stresses. They include materials such as protein hydrolysates, amino acids, humic substances, seaweed extracts and food or industrial waste-derived compounds. Fish processing waste products have potential applications as plant biostimulants. This review gives an overview of plant biostimulants with a focus on fish protein hydrolysates and legislation governing the use of plant biostimulants in agriculture.

Comparative Advantage of Using Biopesticides in Ukrainian Agroecosystems

2020 ◽  
Vol 2 (6) ◽  
Author(s):  
Hasrat Arjjumend ◽  
Konstantia Koutouki ◽  
Olga Donets
Keyword(s):  
Shelf Life ◽  
Crop Production ◽  
Plant Protection ◽  
Agricultural Practices ◽  
Crop Productivity ◽  
Poor Quality ◽  
Soil Biology ◽  
Chemical Contamination ◽  
High Concentration ◽  
Irreversible Damage

The use of unsustainable levels of chemical fertilizers and plant protection chemicals has resulted in a steady decline in soil and crop productivity the world over. Soil biology has undergone irreversible damage, coupled with a high concentration of toxic chemical residues in plant tissues and human bodies. Agricultural practices must evolve to sustainably meet the growing global demand for food without irreversibly damaging soil. Microbial biocontrol agents have tremendous potential to bring sustainability to agriculture in a way that is safe for the environment. Biopesticides do not kill non-target insects, and biosafety is ensured because biopesticides act as antidotes and do not lead to chemical contamination in the soil. This article is part of a larger study conducted in Ukraine by researchers at the Université de Montréal with the support of Mitacs and Earth Alive Clean Technologies. The responses of farmers who use biofertilizers (“user farmers”) and those who do not (“non-user farmers”), along with the responses of manufacturers or suppliers of biofertilizers, and research and development (R&D) scientists are captured to demonstrate the advantages of applying microbial biopesticides to field crops. Participants reported a 15-30% increase in yields and crop production after the application of biopesticides. With the use of biopesticides, farmers cultivated better quality fruits, grains, and tubers with a longer shelf life. Moreover, while the risk of crop loss remains high (60-70%) with chemically grown crops, this risk is reduced to 33% on average if crops are grown using biopesticides. The findings indicate that a large proportion of farmers would prefer to use biopesticides if they are effective and high quality products. In this context, the quality and effectiveness of products is therefore very important. Despite their benefits to soil, human health, and ecosystems, biopesticides face significant challenges and competition vis-à-vis synthetic pesticides for a variety of reasons. Therefore, the development of biopesticides must overcome the problems of poor quality products, short shelf life, delayed action, high market costs, and legal/registration issues.

Diversité floristique spontanée des plantations de café et de cacao dans la forêt classée de Monogaga, Côte d'Ivoire | Spontaneous floristic diversity of cocoa and coffee plantations in the classified forest of Monogaga, Côte d'Ivoire (reviewed paper)

2006 ◽  
Vol 157 (2) ◽  
pp. 31-36 ◽  
Author(s):  
Constant Yves Adou Yao ◽  
Edouard Kouakou N'Guessan
Keyword(s):  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Diversity Index ◽  
Agricultural Practices ◽  
Woody Species ◽  
Secondary Forests ◽  
Cote D’Ivoire ◽  
Coffee Plantations ◽  
Côte D'ivoire ◽  
High Tree Density

The inventory and the analysis of the woody species preserved in the different categories of cocoa and coffee plantations showed that they are relatively diversified (presence of endemic, rare and threatened species). They also preserved high tree density and high basal areas. The young plantations are especially diversified. Their number of species,density and basal areas and diversity index are similar to those of the neighbouring old growth and secondary forests. Older plantations display a sharp decline of diversity over time. Agricultural practices in the region represent a threat in the medium term to the preservation of biodiversity.

Structural Transformation of Punjab Agriculture and its Environmental Implications

2020 ◽  
pp. 533-546
Keyword(s):  
Structural Transformation ◽  
Diversity Index ◽  
Green Revolution ◽  
Agricultural Practices ◽  
Secondary Data ◽  
Cropping Pattern ◽  
Environmental Implications ◽  
Question Mark ◽  
Sustainability Issue ◽  
Economic Survey

As per Economic Survey of Punjab, (2019-20), Punjab alone contributed more than 1/4 and 1/3 of the central pool of rice and th rd wheat respectively in 2018-19 and accordingly named as bread-basket of India. However, such a huge contribution was due to the structural transformation of agriculture in Punjab under the Green Revolution. Such transformation leads to intensive use of natural resources with mono-culture of wheat-paddy till today, which put a big question mark on the sustainability issue of agriculture in Punjab. The present study was exclusively based on secondary data, covering the period of 1965-66 to 2018-2019, almost 55 years. Hirschman Herfindhal index and Simpson Diversity index were used to determine the extent of concentration and diversification in the cropping pattern of Punjab agriculture. The results highlight the facts that level of diversification was declining and the level of concentration was increasing. Moreover, Compound Growth Rate (CGR) of yield was either stagnating or declining over the years. Not only this, based on the index of agriculture production, the trend values were negative since the 21 century. Accordingly, st intensive agricultural practices should be stopped, and diversification should get priority in such a way that food security is not jeopardized.

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