scholarly journals Effect of Starter Culture on the Fatty Acid and Amino Acid Profile of Fermented Parkia biglobosa Seeds

2020 ◽  
Vol 9 ◽  
pp. 15-22
Author(s):  
Ayowole Victor Atere ◽  
Victor O. Oyetayo ◽  
Felix A. Akinyosoye

Iru , a condiment used for seasoning soup is often obtained from the fermentation of Parkiabiglobosa seeds. The present study therefore investigates the effect of different starter culture on the amino and fatty acid profile of Parkiabiglobosa seed fermented to produce iru. Starter culture of Lactobacillus plantarum, Bacillus subtilis and Leuconostoc sp were used to ferment Parkiabiglobosa seeds. The bacterial load increased from 0.00 log10cfu/g in boiled raw sample to 8.67 log10cfu/g in naturally fermented sample. Fermentation significantly (P<0.05) increased the quantity of the amino acids in fermented samples. Aspartic acid (25.85 g/100g) and linoleic acid (42.99 %) were the highest amino and fatty acids respectively in the fermented samples. The result from this study reveals that fermentation significantly increase the amino and fatty acid profile of Parkiabiglobosa fermented to produce iru, a Nigerian condiment.

2018 ◽  
Vol 121 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Ke Wu ◽  
Runying Gao ◽  
Fang Tian ◽  
Yingyi Mao ◽  
Bei Wang ◽  
...  

AbstractThis study quantified the fatty acid profile with emphasis on the stereo-specifically numbered (sn) 2 positional distribution in TAG and the composition of main phospholipids at different lactation stages. Colostrum milk (n 70), transitional milk (n 96) and mature milk (n 82) were obtained longitudinally from healthy lactating women in Shanghai. During lactation, total fatty acid content increased, with SFA dominating in fatty acid profile. A high ratio of n-6:n-3 PUFA was observed as 11:1 over lactation due to the abundance of linoleic acid in Chinese human milk. As the main SFA, palmitic acid showed absolute sn-2 selectivity, while oleic acid, linoleic acid and α-linolenic acid, the main unsaturated fatty acids, were primarily esterified at the sn-1 and sn-3 positions. Nervonic acid and C22 PUFA including DHA were more enriched in colostrum with an sn-2 positional preference. A total of three dominant phospholipids (phosphatidylethanolamine (PE), phosphatidylcholine (PC) and sphingomyelin (SM)) were analysed in the collected samples, and each showed a decline in amount over lactation. PC was the dominant compound followed by SM and PE. With prolonged breast-feeding time, percentage of PE in total phospholipids remained constant, but PC decreased, and SM increased. Results from this study indicated a lipid profile different from Western reports and may aid the development of future infant formula more suitable for Chinese babies.


2011 ◽  
Vol 51 (2) ◽  
pp. 95 ◽  
Author(s):  
O. Dayani ◽  
G. R. Ghorbani ◽  
A. K. Esmailizadeh

Eight multiparous Holstein cows in mid lactation (average days in milking of 160 ± 40) were used in a replicated 4 by 4 Latin square design, each experimental period lasting 3 weeks, to determine the effects of whole cottonseed (WCS) treatment and dietary crude protein (CP) concentration on the profile of milk fatty acids. Each 3-week experimental period consisted of 2 weeks for ration adaptation followed by 1 week for data collection. The experimental diets consisted of: (1) Control (without WCS), 16% CP; (2) 20% WCS, 16% CP; (3) 20% WCS, 13% CP; and (4) 20% crushed WCS, 13% CP. Minerals and vitamin supplements were the same in all experimental diets. The WCS treatment led to increased DM intake, fat-corrected milk yield, fat concentration, proportion of long-chain fatty acids, unsaturated fatty acids and ratio of unsaturated to saturated fatty acids (P < 0.05). Decreased concentration of dietary CP from 16 to 13% (diet 2 compared with diet 3) led to a decrease in both milk yield and milk composition (P < 0.05). WCS crushed in diets with 13% CP (diet 4 compared with diet 3) increased (P < 0.05) the concentration of conjugated linoleic acid in milk fat. The results demonstrated that adding WCS to the diet of lactating cows improves both milk yield and milk fatty acid profile, particularly the proportion of conjugated linoleic acid.


2021 ◽  
Vol 854 (1) ◽  
pp. 012071
Author(s):  
Dejan Peric ◽  
Ivana Brankovic Lazic ◽  
Srdjan Pantic ◽  
Milan Z. Baltic ◽  
Jelena Janjic ◽  
...  

Abstract In monogastric animals, tissue fatty acid profile directly reflects the fatty acid profile present in the animal’s diet. Inadequate ratio of fatty acids in food can lead to negative effects on human health. Conjugated linoleic acid (CLA) is a group of isomers of linoleic acid (C18:2), and its most interesting role is in the prevention of tumors, atherosclerosis and diabetes. CLA is found in ruminant meat and milk, and since pigs and poultry do not have the ability to synthesize CLA, it is possible to add them to animal feed with biotechnological solutions. The scientific public imposes modern parameters for determining the nutritional value of fatty acids, in which the AI – index of atherogenicity, TI – index of thrombogenicity and H/H - hypocholesterolemic/hypercholesterolemic ratio are distinguished. The aim of this study was to determine the effect CLA addition to the diet of non-ruminants on the lipid indices of certain categories of meat, from the aspect of consumer health needs. A significant influence of the correction of feed’s fatty acid composition on the lipid indices in food of animal origin was determined.


Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 297
Author(s):  
Jessica de Souza Vilela ◽  
Tharcilla I. R. C. Alvarenga ◽  
Nigel R. Andrew ◽  
Malcolm McPhee ◽  
Manisha Kolakshyapati ◽  
...  

We evaluated the effects of full-fat black soldier fly larvae (BSFL) on broiler carcass composition, cut yield, and breast meat quality. Broilers were fed for 42 days with up to 20% dietary inclusion of BSFL (0, 5, 10, 15, and 20%). On day 42, 120 broilers were slaughtered, and images were taken using computed tomography. Breasts, drumsticks, and thighs were collected for cut yield determination. The pH, color, lipid oxidation, cooking loss, shear force, amino acid profile, and fatty acid profile of the breast meat were assessed. There was no dietary effect on carcass composition or meat quality parameters except for fatty and amino acids compositions. When 20% BSFL was included in the diet, individual fatty and amino acids, such as lauric and myristic acids, aspartic acid, glutamine, and lysine, increased by 22.0-, 5.50-, 1.08-, 1.06-, and 1.06-fold, respectively (p < 0.05). Although total polyunsaturated fatty acids decreased, eicosapentaenoic fatty acids (EPA) increased by 78% in the 20% BSFL inclusion group. In conclusion, up to 20%, dietary full-fat BSFL did not affect key meat characteristics but positively increased the levels of the health-claimable omega-3 fatty acid EPA.


2013 ◽  
Vol 58 (No. 7) ◽  
pp. 328-341 ◽  
Author(s):  
M. Czauderna ◽  
J. Kowalczyk ◽  
M. Marounek

The influence of selenite (Se<sup>IV</sup>) or selenate (Se<sup>VI</sup>) added to ovine ruminal fluid containing linseed oil (LO) on the profile of fatty acids (FA), particularly conjugated linoleic acid (CLA) isomers, was investigated. The ruminal fluid was incubated in vitro at 39&deg;C under CO<sub>2</sub> either alone (the control fluid) or with LO (3.3 mg/ml) or with a combination of LO with either a low (0.167 &mu;g/ml) or high (1.67 &mu;g/ml) level of Se as Se<sup>IV</sup> or Se<sup>VI</sup>. LO added to ruminal fluids also provides an extra source of energy. The tubes with the examined fluids were removed after 0, 6, 12, 18, or 24 h of in vitro incubation and then analyzed to determine the FA levels. The lower and higher concentration of Se<sup>IV </sup>in the fluids with the LO revealed negligible effect on the concentration of the sum of the CLA isomers (&sum;CLA) in the fluid compared with the fluid with LO alone. The addition of a higher amount of Se<sup>IV</sup> to the fluid containing LO usually decreased the concentration of &sum;CLA compared with the fluid containing the lower concentration of Se<sup>IV</sup> and LO. The concentration of c9t11c15C18:3 (cLNA) in the fluids with LO, irrespective of the presence of extra Se, increased throughout the incubations, although the addition of Se<sup>IV </sup>or Se<sup>VI</sup> to the fluids containing LO numerically reduced the increase of the concentration of cLNA compared with the fluid with LO alone. The concentration sum of the C18:1 isomers (&Sigma;C18:1) in the control fluid numerically decreased throughout the incubations, while LO added to the fluid increased the concentration of &Sigma;C18:1 throughout the incubations. LO added to the fluid, irrespective of the presence of Se<sup>IV</sup> or Se<sup>VI</sup>, significantly increased the concentration of &Sigma;C18:1 compared with the control fluid and the fluids with Se<sup>IV </sup>or Se<sup>VI</sup>. The concentrations of C16:0 and C18:0 in the control fluid and the fluids containing Se<sup>IV</sup> or Se<sup>VI </sup>numerically increased throughout the incubations and were usually lower than in the fluids containing LO without or with Se<sup>IV </sup>or Se<sup>VI</sup>. The concentration of C18:3n-3 decreased throughout the incubation of the fluids containing LO, irrespective of the presence of Se<sup>IV </sup>or Se<sup>VI</sup>. LO added to the fluids, irrespective of the presence of Se<sup>IV</sup> or Se<sup>VI</sup>, increased the concentration of C18:2n-6 compared with the control fluid and the fluids with Se<sup>IV </sup>or Se<sup>VI</sup>. The higher concentration of Se<sup>IV</sup> or Se<sup>VI</sup> in the fluid with LO most efficiently increased the concentration of c5c8c11c14c17C20:5 compared with the control fluid or the fluids containing LO, irrespective of the presence of the lower concentration of Se<sup>IV</sup> or Se<sup>VI</sup>. LO added to the fluid, irrespective of the presence of Se<sup>IV </sup>or Se<sup>VI</sup>, increased the concentration of polyunsaturated FA compared with the control fluid or the fluids containing Se<sup>IV </sup>or Se<sup>VI</sup>. &nbsp; &nbsp;


2018 ◽  
Vol 72 ◽  
pp. 1-6
Author(s):  
Aygul Dayan ◽  
Nebahat Sari ◽  
Fatih Özogul

In this study, Nusem and Beppo snack seed pumpkin cultivars were used to determine the effects of different plant activators on seed protein, lipid and fatty acids contents. In the context of study, plant activators consist of Crop-set (CR), EM1, ERS, Vitormone-Plus Drip (VIT), Bacillus subtilis (OSU 142), Bacillus megatorium (M3), Azospirillum sp. (SP 245), Spirulina platensis (SIP), Ecocompost (EKO), Camli Botanica liquid organic fertilizer (BOT) and Zincon (ZIN) were used as organic fertilizer. In the experiment, the plant activators were applied to the plants alone or in combination with each other and organic fertilizer. Two separate control groups which were organic and conventional (CONV.) fertilizer have been identified. As a result of the use of different plant activators, the highest protein content was obtained from CONV. application (35.50%), M3+SP 245 (33.09%) and M3 (33.04%); the highest lipid content was observed from SP 245+OG (45.90%), CR (44.48%) and SIP+OG (44.26%) applications. The use of different plant activators effected the fatty acid contents of seeds. Total 11 fatty acids were identified. Among the fatty acids, C16:0 (Palmitic acid), C18:0 (Stearic acid), C18:1 (Oleic acid) and C18:2 (Linoleic acid) were found dominant.


2021 ◽  
Vol 47 ◽  
pp. e618
Author(s):  
Katia Rodrigues Batista de OLIVEIRA ◽  
Daniel Abreu Vasconcelos CAMPELO ◽  
Weliton Pedro BATISTON ◽  
Wilson Massamitu FURUYA ◽  
Jener Alexandre Sampaio ZUANON ◽  
...  

The effects of feeding diets with conjugated linoleic acid (CLA) to Astyanax altiparanae for different periods were investigated on carcasses’ fatty acid profile and nutritional quality. The trial was laid out in a complete randomized design with eight feeding periods (0, 7, 14, 21, 28, 35, 42, and 49 days) and four replicates. A total of 240 fish (3.4 ± 0.3 g) were distributed into four aquariums and fed with the diet containing 32% crude protein, 15 MJ kg-1 gross energy, and 2.5% of CLA. Feeding CLA diet for 49 days resulted in the higher deposition of CLA, CLA isomers, and docosahexaenoic acid (DHA). The isomer c9,t11 was highly deposited than the t10,c12. Polyunsaturated fatty acids (PUFA), n-3, and n-6 increased with feeding period, whereas saturated (SFA), monounsaturated, and medium-chain fatty acids decreased. PUFA/SFA ratio, DHA/EPA, EPA+DHA, and thrombogenicity index increased linearly. Whereas atherogenicity index reduced, hypocholesterolemic/hypercholesterolemic ratio increased in quadratic effects over feeding time. No difference was observed for the n-6/n-3 ratio. Feeding A. altiparanae with 2.5% of CLA for a minimum of 35 days improves the fatty acid profile and provides a commercial product with good nutritional quality and functional benefits.


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