scholarly journals Antimicrobial properties of silver nanoparticles against biofilm formation by Pseudomonas aeruginosa on archaeological textiles

2016 ◽  
Vol 1 (2) ◽  
pp. 1 ◽  
Author(s):  
Katarzyna Pietrzak ◽  
Anna Otlewska ◽  
Michał Puchalski ◽  
Beata Gutarowska ◽  
Patricia Guiamet
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Ribosomal Rna ◽  
Biofilm Formation ◽  
Antimicrobial Properties ◽  
Brown Pigment ◽  
Pseudomonas Sp ◽  
Green Pigment ◽  
X Ray ◽  
Bluish Green

The aims of this work were to: (i) microscopically analyse the pre- and post-Columbian archaeological textiles using Scanning Electron Microscopy with Energy Dispersive X-Ray Analysis (SEM-EDX); (ii) microbiologically analyse the archaeological textiles (from the Southern Andean Area, La Plata Museum); (iii) determine the ability of Pseudomonas sp. isolates from archaeological textiles to biofilm formation by SEM; (iv) assess the anti-biofilm properties of AgNPs protecting cotton against Pseudomonas sp. Results showed the presence of bacteria with proteolytic and lipolytic activities on archaeological textiles, including Clostridium sp. and Pseudomonas aeruginosa. Two nucleotide sequences of 16S ribosomal RNA gene of P. aeruginosa strains were deposited in GeneBank NCBI database with accession numbers: KP842564 (strain 1) and KP842565 (strain 2). Those strains exhibited different morphological and growth characteristics: strain 1 with ability to form biofilms on archaeological textiles was rod-shaped, produced bluish-green pigment, and smaller than strain 2; and strain 2 was pleomorphic and produced brown pigment. The use of silver nanoparticles (90 ppm, φ 10–80 nm) allowed to protecting textiles against P. aeruginosa growth by 63%–97%, depending on the strain and exposition time.

Antimicrobial properties of silver nanoparticles against biofilm formation by Pseudomonas aeruginosa on archaeological textiles

2017 ◽  
Vol 2 (1) ◽  
pp. 1-9
Author(s):  
Katarzyna Pietrzak ◽  
◽  
Anna Otlewska ◽  
Michał Puchalski ◽  
Beata Gutarowska ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Biofilm Formation ◽  
Antimicrobial Properties

scholarly journals Antimicrobial properties of silver nanoparticles against biofilm formation by Pseudomonas aeruginosa on archaeological textiles

2017 ◽  
Vol 2 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Katarzyna Pietrzak ◽  
◽  
Anna Otlewska ◽  
Michał Puchalski ◽  
Beata Gutarowska ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Biofilm Formation ◽  
Antimicrobial Properties

Silver nanoparticles impede the biofilm formation by Pseudomonas aeruginosa and Staphylococcus epidermidis

2010 ◽  
Vol 79 (2) ◽  
pp. 340-344 ◽  
Author(s):  
Kalimuthu Kalishwaralal ◽  
Selvaraj BarathManiKanth ◽  
Sureshbabu Ram Kumar Pandian ◽  
Venkataraman Deepak ◽  
Sangiliyandi Gurunathan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation

Antimicrobial Activities of Coriander Seed Essential Oil and Silver Nanoparticles

2021 ◽  
Author(s):  
Kadriye Ozlem saygi ◽  
Birgul Kacmaz ◽  
Serdar Gul
Keyword(s):  
Silver Nanoparticles ◽  
Essential Oil ◽  
Bacterial Infections ◽  
Materials Science ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Elemental Distribution ◽  
Sem Images ◽  
X Ray ◽  
Coriander Seed

Abstract Bacterial infections are one of the most serious health problems all over the world, which cause need for the discovery of new drug. Since antibiotic resistance is a major threat to both humans and the environment, there is a need for studies on the antimicrobial properties of different forms of traditionally used plants. Herein, the seeds of coriander were used to isolate essential oil (EO) and to synthesise silver nanoparticles (C-AgNPs). The major oil constituents were characterized by GC-MS as Linalool (79.12%), Camphor (6.16%), γ-Terpinene (2.82%) and α-Pinene (2.67%). The synthesized AgNPs were characterized by UV-Visible spectrophotometry, Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX), X-ray difraction (XRD) and Fourier Tranmission Infrared (FTIR). The surface plasmon resonance (SPR) of C-AgNPs at 437 nm was recorded on the UV-Vis spectrometer. The spherical and homogenous of AgNPs was presented in SEM images. EDX showed elemental distribution and confirmed AgNPs. A characteristic intense peak was at 3.0 keV. The antibacterial activities of the essential oil and AgNPs form of coriander seed against Staphylococcus aureus ATCC 29213 and Escherichia coli ATCC 25922 bacteria were investigated by broth microdilution test. AgNPs and essential oil of coriander can be expected to provide future opportunities in nanomedicine and materials science. AgNPs can be displayed synergistic antimicrobial effect when used in combination with essential oil.

In vitro analysis of green fabricated silver nanoparticles (AgNPs) against Pseudomonas aeruginosa PA14 biofilm formation, their application on urinary catheter

2021 ◽  
Vol 151 ◽  
pp. 106058
Author(s):  
Felix LewisOscar ◽  
Chari Nithya ◽  
Sasikumar Vismaya ◽  
Manivel Arunkumar ◽  
Arivalagan Pugazhendhi ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Biofilm Formation ◽  
Urinary Catheter ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Exploiting the Potential of Moringa oleifera Oil/Polyvinyl Chloride Polymeric Bionanocomposite Film Enriched with Silver Nanoparticles for Antimicrobial Activity

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Musarat Amina ◽  
Nawal M. Al Musayeib ◽  
Nawal A. Alarfaj ◽  
Maha F. El-Tohamy ◽  
Hisham E. Orabi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Polyvinyl Chloride ◽  
Moringa Oleifera ◽  
Shigella Flexneri ◽  
Antimicrobial Properties ◽  
Salmonella Typhi ◽  
Gas Chromatography Mass Spectrometry ◽  
X Ray ◽  
Different Strains

The present study focused on the prospect of fabricating a polymeric naturally extracted Moringa oleifera oil bionanocomposite film enriched with silver nanoparticles for antimicrobial activity. In this study, a standard concentration of Moringa oleifera oil (5-10 wt%) was used to fabricate a polymeric bionanocomposite film using polyvinyl chloride (PVC) enriched with silver nanoparticles. The active constituents of the extracted Moringa oleifera oil were verified using gas chromatography-mass spectrometry. Spectroscopic and microscopic techniques, including scanning electron microscopy, X-ray diffraction, and energy-dispersive X-ray analysis, were employed to characterize and study the surface morphology of the fabricated bionanocomposite film. The antimicrobial activity of the fabricated bionanocomposite film was investigated using different strains of bacteria and fungus. The results revealed well-oriented and excellently dispersed silver nanoparticles in the PVC-Moringa oleifera oil matrix. The bionanocomposite was able to inhibit the growth of Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Pseudomonas aeruginosa, Shigella flexneri, and Candida albicans. The combination of nanoparticles with polymers is opening new routes for engineering fixable composites, which showed antimicrobial properties.

scholarly journals Bacterial plasma membrane is the main cellular target of silver nanoparticles in Escherichia coli and Pseudomonas aeruginosa

2018 ◽  
Author(s):  
Olesja M Bondarenko ◽  
Mariliis Sihtmäe ◽  
Julia Kuzmičiova ◽  
Lina Ragelienė ◽  
Anne Kahru ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Plasma Membrane ◽  
Antimicrobial Properties ◽  
Consumer Products ◽  
Minimal Bactericidal Concentration ◽  
Flow Cytometry Analysis ◽  
E Coli ◽  
Cellular Target

ABSTRACTSilver nanoparticles (AgNP) are widely used in consumer products, mostly due to their excellent antimicrobial properties. One of the well-established antibacterial mechanisms of AgNP is their efficient contact with bacteria and dissolution on cell membranes. To our knowledge, the primary mechanism of cell wall damage and the event(s) initiating bactericidal action of AgNP are not yet elucidated.In this study we used a combination of different assays to reveal the effect of AgNP on i) bacterial envelope in general, ii) outer membrane (OM) and iii) on plasma membrane (PM). We showed that bacterial PM was the main target of AgNP in Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. AgNP depolarized bacterial PM, induced the leakage of the intracellular K+, inhibited respiration and caused the depletion of the intracellular ATP. In contrast, AgNP had no significant effect on the bacterial OM. Most of the adverse effects on bacterial envelope and PM occurred within the seconds and in the concentration range of 7-160 μM AgNP, depending on the bacteria and assay used, while irreversible inhibition of bacterial growth (minimal bactericidal concentration after 1-h exposure of bacteria to AgNP) occurred at 40 μM AgNP for P. aeruginosa and at 320 μM AgNP for E. coli.Flow cytometry analysis showed that AgNP were binding to P. aeruginosa but not to E. coli cells and were found inside the P. aeruginosa cells. Taking into account that AgNP did not damage OM, we speculate that AgNP entered P. aeruginosa via specific mechanism, e.g., transport through porins.

scholarly journals Facile and Eco-Friendly Fabrication of Silver Nanoparticles Using Nyctanthes arbor-tristis Leaf Extract to Study Antibiofilm and Anticancer Properties against Candida albicans

2022 ◽  
Vol 2022 ◽  
pp. 1-10
Author(s):  
Beema Shafreen Rajamohamed ◽  
Seema Siddharthan ◽  
Velmurugan Palanivel ◽  
Mohanavel Vinayagam ◽  
Vijayanand Selvaraj ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Candida Albicans ◽  
Biofilm Formation ◽  
Leaf Extract ◽  
Morphological Changes ◽  
Spherical Shape ◽  
Toxic Chemicals ◽  
X Ray ◽  
Anticancer Properties ◽  
Color Visualization

The synthesis of silver nanoparticles has been gaining more attention in recent years due to their small size and high stability. For this study, silver nanoparticles were biosynthesized from leaf extract of the medicinal plant (N. arbor-tristis). Vitally, the shrub with tremendous medicinal usage was diversely observed in South Asia and South East Asia. The synthesized silver nanoparticles were characterized by color visualization, ultraviolet-visible spectrophotometry (UV-Vis), Fourier-transform infrared spectroscopy (FTIR), field emission-scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDX), and dynamic light scattering (DLS) technique. A sharp peak at 427 nm for biosynthesized nanoparticles was obtained using UV-Vis, which represents surface plasmon resonance. Thus, characterization techniques showed the green synthesis of AgNPs leads to the fabrication of spherical shape particles with a size of 67 nm. Furthermore, AgNPs were subjected to antibiofilm studies against Candida albicans and it was observed that 0.5 μg mL−1 of AgNPs significantly reduced 50% of biofilm formation. These biosynthesized nanoparticles also showed a considerable reduction in viability of HeLa cells at 0.5 μg mL−1. The morphological changes induced by AgNPs were observed by AO/EB staining. The toxic effect of AgNPs was studied using brine shrimp as a model system. Therefore, it is envisaged that further investigation with these AgNPs can replace toxic chemicals, assist in the development of biomedical implants that can prevent biofilm formation, and avoid infections due to C. albicans.

scholarly journals Characterization of Silver Nanoparticles Obtained by a Green Route and Their Evaluation in the Bacterium of Pseudomonas aeruginosa

Crystals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 395 ◽  
Author(s):  
Juan Carlos Martínez Espinosa ◽  
Raúl Carrera Cerritos ◽  
Maria Antonieta Ramírez Morales ◽  
Karla Paola Sánchez Guerrero ◽  
Rocio Alejandra Silva Contreras ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Photoelectron Spectroscopy ◽  
Bacterial Resistance ◽  
Antimicrobial Effect ◽  
X Ray ◽  
Transmission Electron ◽  
Green Route

Metal nanoparticles are widely used in different areas such as biotechnology and biomedicine, for example in drug delivery, imaging and control of bacterial growth. The antimicrobial effect of silver has been identified as an alternative approach to the increasing bacterial resistance to antibiotics. Silver nanoparticles were synthesized by the green route using the Geranium extract as a reducing agent. The characterization was carried out by the techniques of UV-Vis spectrophotometry, transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), X-ray emitted photoelectron spectroscopy (XPS) and X-ray diffraction. Nanoparticle diameters between 15 and 50 nm were obtained and the interplanar spaces calculated from the electron diffraction pattern corresponding to a mixture of silver with 4H and FCC structures. To determine the minimum inhibitory concentration of silver nanoparticles (AgNPs) on the Pseudomonas aeruginosa bacteria (ATCC-27853), different concentrations of colloidal solution 0.36, 0.18, 0.09 and 0.05 μg/mL were evaluated as a function of the incubation time, measuring the inhibition halo and colony forming unit (CFU) during 0, 2 and 4 h of incubation. The minimum inhibitory AgNPs concentration (MIC) is 0.36 μg/mL at 0 h while the concentration of 0.18 μg/mL presents a total inhibition of the bacterium after 2 h. For the rest of the dilutions, gradual inhibitions as a function of time were observed. We evaluate the antibacterial effect of silver nanoparticles obtained by a green methodology in Pseudomonas aeruginosa bacteria. Finally, the colloidal nanoparticle solution can be an antibacterial alternative for different biomedical approaches.

scholarly journals Electrophoretic Deposition of Gentamicin-Loaded ZnHNTs-Chitosan on Titanium

Coatings ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 944
Author(s):  
Ahmed Humayun ◽  
Yangyang Luo ◽  
David K. Mills
Keyword(s):  
Biofilm Formation ◽  
Bacterial Colonization ◽  
Antimicrobial Properties ◽  
Halloysite Nanotubes ◽  
Visible Spectroscopy ◽  
Sustained Drug Release ◽  
X Ray ◽  
Implant Coating ◽  
Uv Visible ◽  
Implant Coatings

There is a need for titanium (Ti), an antimicrobial implant coating that provides sustained protection against bacterial infection. Chitosan (CS) coatings, combined with halloysite nanotubes (HNTs), are an attractive solution due to the inherent biocompatibility of halloysite, its ability to provide sustained drug release, and the antimicrobial properties of CS. In this study, the electrodeposition (EPD) method was used to coat titanium foil with CS blended with zinc-coated HNTs (ZnHNTs) and pre-loaded with the antibiotic gentamicin. The CS-ZnHNTs-gentamycin sulfate (GS) coatings were characterized using scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), X-ray powder diffraction (XRD), X-ray fluorescence (XRF), Fourier-transform infrared spectroscopy (FTIR), and UV-visible spectroscopy. The coatings were further examined for their ability to sustain GS release, resist bacterial colonization and growth, and prevent biofilm formation. The CS-ZnHNTs-GS coatings were cytocompatible, exhibited significant antimicrobial properties, and supported pre-osteoblast cell proliferation. Hydroxyapatite also formed on the coatings after immersion in simulated body fluid. While the focus in this study was on zinc-coated HNTs doped into CS, our design offers tunability, as different metals can be coated onto the HNT surface and different drugs or growth factors loaded into the HNT lumen. Our results, and the potential for customization, suggest that these coatings have potential in the construction of an array of infection-resistant implant coatings.

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