scholarly journals Comparing the effect of statins on hepatic fibrosis induced by carbon tetrachloride in Wistar rats

2021 ◽  
Vol 11 (1) ◽  
pp. 35
Author(s):  
Anupama Dhavaleshwar ◽  
Bharti Chogtu ◽  
Deepak Nayak ◽  
Praveen Kumar S. E.
Keyword(s):  
Carbon Tetrachloride ◽  
Hepatic Fibrosis ◽  
Liver Enzymes ◽  
Liver Toxicity ◽  
Corn Oil ◽  
Methyl Cellulose ◽  
Group Iii ◽  
Intergroup Comparison ◽  
The Difference

Background: The clinical studies have shown contrary results regarding hepatoprotective effect of statins. However, antifibrotic properties of statins in in vitro and in vivo experimental models have been demonstrated. The purpose of this study was to assess and compare the effect of statins on serum liver enzymes and their antifibrotic effects.Methods: Forty two rats were divided into 7 groups (I to VII) (n=6). Liver toxicity was induced by injecting carbon tetrachloride (1 ml/kg). Control groups received corn oil (0.1 ml/100 gm) and carboxy methyl cellulose (0.50%) respectively. Group III to VII received carbon tetrachloride (CCl4) for 6 weeks and then groups IV, V, VI and VII received simvastatin (10 mg/kg), atorvastatin (15 mg/kg), rosuvastatin (2 mg/kg) and silymarin (50 mg/kg) for another 8 weeks respectively. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) levels were estimated in all the groups at baseline, 6 weeks and 14 weeks. At 14 weeks, histopathology of liver was done in all groups.Results: At 14 weeks, all the test groups (IV, V and VI) showed a significant decrease in serum ALT, AST and ALP levels as compared to control (p<0.05) and group III (p<0.05). On intergroup comparison, liver enzymes in rats in group VI (rosuvastatin) and group V (atorvastatin) were decreased more in comparison to group IV (simvastatin) but the difference was not statistically significant except for AST levels where the difference was significant between the statins. There was decrease in hepatic fibrosis by statins with rosuvastatin being superior followed by atorvastatin and simvastatin.Conclusions: In the present study statins decreased the serum AST, ALT and ALP levels and histopathological changes were reversed by statins in CCl4 induced hepatotoxic models.

scholarly journals Hepatoprotective effect of galangin on carbon tetrachloride-induced hepatotoxicity via the LKB1/AMPK pathway

2021 ◽  
Vol 19 ◽  
pp. 205873922110008
Author(s):  
Meng Chen ◽  
Xinyan Song ◽  
Jifang Jiang ◽  
Lei Xing ◽  
Pengfei Wang
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Toxicity ◽  
Corn Oil ◽  
Histopathological Examination ◽  
Hepatoprotective Effect ◽  
Control Group ◽  
Group Model ◽  
Protective Effects ◽  
Model Group ◽  
Expression Levels

To investigate the protective effects of galangin on liver toxicity induced by carbon tetrachloride (CCl4) in mice. Mouse hepatotoxicity model was established by intraperitoneal injection (i.p.) of 10 ml/kg body weight CCl4 that diluted with corn oil to a proportion of 1:500 on Kunming mice. The mice were randomly divided into five groups named control group, model group, and 1, 5, and 10 mg/kg galangin group. The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were analyzed by ELISA. Liver histopathological examination was observed via optical microscopy. The levels of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), and glutathion (GSSG) were analyzed to assess oxidative stress. Finally, western blot assay was carried out to analyse the expression levels of total AMP-activated protein kinase (AMPK), phospho-AMPK (p-AMPK), total liver kinase B1 (LKB1), and phospho-LKB1 (p-LKB1). Compared with the control group, in the model group, the levels of AST, ALT, MDA, and GSSG increased significantly ( p < 0.01); the activity of SOD and GSH decreased significantly ( p < 0.01); and the histopathological examination revealed liver necrosis. However, treatment with galangin (5 and 10 mg/kg) significantly reversed these CCl4-induced liver damage indicators. Furthermore, treatment with galangin (10 mg/kg) significantly increased the p-AMPK and p-LKB1 expression levels ( p < 0.01). This study supports the hepatoprotective effect of galangin against hepatotoxicity, perhaps occurring mainly through the LKB1/AMPK-mediated pathway.

A nutrient mixture suppresses carbon tetrachloride–induced acute hepatic toxicity in ICR mice

2008 ◽  
Vol 27 (7) ◽  
pp. 559-566 ◽  
Author(s):  
MW Roomi ◽  
T Kalinovsky ◽  
NW Roomi ◽  
V Ivanov ◽  
M Rath ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Toxicity ◽  
Corn Oil ◽  
Icr Mice ◽  
Renal Markers ◽  
Serum Aspartate Aminotransferase ◽  
Liver And Kidney ◽  
Tea Extract ◽  
Kidney Functions ◽  
Group D

We examined the effect of a nutrient mixture (NM) that contains lysine, proline, ascorbic acid, and green tea extract in mice treated with carbon tetrachloride (CCl4), a model of liver injury in which free radical, oxidative stress, and cytokine production are closely linked. Seven-week-old male Imprinting Control Region (ICR) mice were divided into four groups (A–D) of five animals each. Groups A and C mice were fed a regular diet for 2 weeks, whereas groups B and D mice were supplemented with 0.5% NM (w/w) during that period. Groups A and B received corn oil i.p., whereas groups C and D received CCl4 (25 μL/kg, in corn oil, i.p.). All animals were killed 24 h after CCl4 administration, serum was collected to assess liver and kidney functions, and livers and kidneys were excised for histology. Mean serum aspartate aminotransferase and alanine aminotransferase were comparable in groups A and B, increased markedly in group C, and significantly lowered in group D compared with group C. CCl4 had no significant effect on renal markers (blood urea nitrogen [BUN], creatinine, and BUN/creatinine ratio). CCl4 administration caused an intense degree of liver necrosis that was less severe in the NM fed group D. These results indicate that NM could be a useful supplement in preventing acute chemical-induced liver toxicity.

scholarly journals Autosomal dominant erythrocytosis caused by increased sensitivity to erythropoietin

Blood ◽  
1991 ◽  
Vol 78 (11) ◽  
pp. 3066-3069 ◽  
Author(s):  
E Juvonen ◽  
E Ikkala ◽  
F Fyhrquist ◽  
T Ruutu
Keyword(s):  
Autosomal Dominant ◽  
Family Members ◽  
Calf Serum ◽  
Methyl Cellulose ◽  
Colony Growth ◽  
Erythroid Progenitors ◽  
Obvious Effect ◽  
Increased Sensitivity ◽  
The Difference

Abstract We describe here a family with autosomal dominant erythrocytosis. In in vitro cultures, performed using the methyl cellulose assay, the number of erythroid colonies was normal or marginally increased when a standard concentration of erythropoietin (Epo) was used, but at lower Epo concentrations, the investigated persons formed more colonies than the controls. The difference was generally greater the lower the Epo concentration became. Some erythroid colony growth was seen even in the absence of any added Epo (apart from the minute concentration found in fetal calf serum), a phenomenon not seen in the controls. This finding indicates that the erythrocytosis in this family is caused by hypersensitivity of erythroid progenitors to Epo. The serum Epo concentration was low or low normal in all of the investigated family members, which is in good accordance with hypersensitivity to Epo. The erythrocytosis has not had any obvious effect on the health or life- span of the affected individuals. Many of them have reached an advanced age, and one of the affected family members has won several Olympic gold medals and world championships in endurance sports.

scholarly journals An In Vitro Comparison of the Apical Seal Produced by Three Different Thermoplasticized Guttapercha Obturation Techniques in Oval Canals

2015 ◽  
Vol 03 (01) ◽  
pp. 039-046
Author(s):  
Geetanjali Bansal ◽  
Ajay Bansal ◽  
Bhupinder Padda
Keyword(s):  
Control Group ◽  
Group Iii ◽  
Dye Penetration ◽  
Sealing Ability ◽  
India Ink ◽  
Nail Polish ◽  
Group I ◽  
Group Ii ◽  
The Difference

AbstractThe aim of this study was to evaluate and compare the sealing ability to obturate oval canals with three thermoplasticizedguttaperchaobturation techniques taking lateral condensation technique as the control. Ninety-five freshly extracted teeth were decoronated at 2mm coronal to CEJ. Biomechanical preparation was done using step back technique. The teeth were divided into three experimental groups of 30 teeth each and one control group of 5 teeth. The group I (control group) was obturated with lateral condensation technique, group II obturated with injectable thermoplasticizedguttapercha technique, group III obturated with thermoplasticizedguttapercha with downpack and backfill technique andgroup IV obturated with core carrier thermoplasticizedguttapercha technique. The sealability of each technique was assessed by a dye penetration method. The roots were given two full layers of nail polish varnish except apical 2mm. Specimens were then immersed in India ink for 48 hours. Robertson’s technique was used to clear the specimens. The linear dye penetration was measured from anatomic apex to the deepest extent of dye penetration in a coronal direction using triocular stereomicroscope at 10 × magnification. The mean dye leakage of group I was 2.6700mm;group II 0.1713mm;group III 3.3977mm; group IV 2.3210mm. When the means of all the four groups were compared using Kruskal Wallis test the difference was found to be very highly significant with the value<.001**, meaning there by that group II is significantly better than the other three groups as far as sealing ability is concerned.

Autosomal dominant erythrocytosis caused by increased sensitivity to erythropoietin

Blood ◽  
1991 ◽  
Vol 78 (11) ◽  
pp. 3066-3069 ◽  
Author(s):  
E Juvonen ◽  
E Ikkala ◽  
F Fyhrquist ◽  
T Ruutu
Keyword(s):  
Autosomal Dominant ◽  
Family Members ◽  
Calf Serum ◽  
Methyl Cellulose ◽  
Colony Growth ◽  
Erythroid Progenitors ◽  
Obvious Effect ◽  
Increased Sensitivity ◽  
The Difference

We describe here a family with autosomal dominant erythrocytosis. In in vitro cultures, performed using the methyl cellulose assay, the number of erythroid colonies was normal or marginally increased when a standard concentration of erythropoietin (Epo) was used, but at lower Epo concentrations, the investigated persons formed more colonies than the controls. The difference was generally greater the lower the Epo concentration became. Some erythroid colony growth was seen even in the absence of any added Epo (apart from the minute concentration found in fetal calf serum), a phenomenon not seen in the controls. This finding indicates that the erythrocytosis in this family is caused by hypersensitivity of erythroid progenitors to Epo. The serum Epo concentration was low or low normal in all of the investigated family members, which is in good accordance with hypersensitivity to Epo. The erythrocytosis has not had any obvious effect on the health or life- span of the affected individuals. Many of them have reached an advanced age, and one of the affected family members has won several Olympic gold medals and world championships in endurance sports.

scholarly journals Effectiveness of Combination Irrigant Technique for Debris Removal in Simulated Canal Irregularities: An in vitro Study

2019 ◽  
Vol 4 (2) ◽  
pp. 79-84
Author(s):  
S Dhivyalakshmi ◽  
◽  
N Velmurugan ◽  
G Lakshmanan ◽  
L Karthik ◽  
...  
Keyword(s):  
Digital Camera ◽  
In Vitro Study ◽  
Combination Group ◽  
P Value ◽  
Chi Square ◽  
Group Iii ◽  
Group I ◽  
Intergroup Comparison ◽  
Before And After

Aim: To evaluate the debridement efficacy of different irrigation systems in simulated canal irregularities. Study Design and Setting: About sixty maxillary central incisors were selected and after chemomechanical preparation were split longitudinally into two halves. Each groove and depression were filled with dentine debris mixed with 2.5 % NaOCl to simulate a situation where dentine debris accumulates in the un-instrumented extensions of the root canal. Materials and Methods: The specimens were randomly divided into 4 experimental groups as follows: Group I - Conventional Needle Irrigation, Group II - Apical Negative Pressure Irrigation (EndoVac), Group III - Passive Ultrasonic Irrigation and Group IV - Combination Irrigation (EV+PUI). 9 ml of 2.5 % NaOCl, 9 ml of 17 % EDTA and 9 ml of saline was used for the final irrigation protocol. Images were taken before and after irrigation using PRIMA DNT surgical microscope with digital camera. Statistical Analysis: The intergroup and individual comparison of percentage reduction of debris in groove and depressions were carried out by using Kruskal-Wallis and Mann-Whitney U tests respectively. The intergroup comparison for different scoring criteria was compared using Pearson’s chi-square tests. Differences were considered statistically significant at P value < 0.05. Results: The combination group, EV group and PUI group showed better removal of debris in Groove and Depressions than Conventional needle irrigation group. The Combination group had significant reduction of debris at 2 mm level when compared to all the other groups. Conclusion: The effectiveness of this combination irrigant delivery system for smear layer removal and antibacterial efficacy have to be evaluated further in future clinical studies

Amelioration of Experimental Hepatotoxicity in Rats by Portulaca oleracea Linn. from Kashmir Himalaya

2021 ◽  
Vol 24 ◽  
Author(s):  
Mohd Akbar Dar ◽  
Prince Ahad Mir ◽  
Mubashir Hussain Masoodi ◽  
Ali S. Alqahtani ◽  
Nasir A. Siddiqui ◽  
...  
Keyword(s):  
Free Radical ◽  
Carbon Tetrachloride ◽  
Liver Diseases ◽  
Methanol Extract ◽  
Liver Toxicity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Portulaca Oleracea ◽  
Serum Glutamic Oxaloacetic Transaminase

Background: Traditionally, Portulaca oleracea Linn for treating abscesses, dysentery, and also for the treatment of liver diseases. Recent studies have reported its efficacy as neuroprotective, analgesic and anti-inflammatory, a bronchodilator, anticancer, antioxidant, wound healing, besides other important pharmacological actions. Aim and Objective: For curing liver diseases, Chinese use juice from the fresh leaves of Portulaca oleracea Linn, hence to prove this claim an in-vivo hepatoprotective study of extracts from Portulaca oleracea Linn. against carbon tetrachloride hepatotoxic rats. Methods: The in-vitro antioxidant activity by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and hepatoprotective activity of extracts of Portulaca oleracea Linn. was assessed against the carbon tetrachloride-induced toxicity rat model. Results: The extracts of Portulaca oleracea Linn produced concentration-dependent percentage inhibition of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical and most significantly by methanol extract. Among the extracts, methanol extract showed a significant decrease in Serum glutamic oxaloacetic transaminase (SGOT), Serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP) levels, and a significant increase in total protein (TP) levels against carbon tetrachloride-induced liver toxicity. Conclusion: The results obtained showed that methanol extract of Portulaca oleracea Linn. possesses significant DPPH free radical scavenging and hepatoprotective potential.

scholarly journals Investigation of Antioxidant and Hepatoprotective Activity of StandardizedCurcuma xanthorrhizaRhizome in Carbon Tetrachloride-Induced Hepatic Damaged Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Sutha Devaraj ◽  
Sabariah Ismail ◽  
Surash Ramanathan ◽  
Mun Fei Yam
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Functional Food ◽  
Liver Enzymes ◽  
Food Supplement ◽  
Hepatoprotective Activity ◽  
Hexane Fraction ◽  
Good Recovery ◽  
Traditional System

Curcuma xanthorrhiza(CX) has been used for centuries in traditional system of medicine to treat several diseases such as hepatitis, liver complaints, and diabetes. It has been consumed as food supplement and “jamu” as a remedy for hepatitis. Hence, CX was further explored for its potential as a functional food for liver related diseases. As such, initiative was taken to evaluate the antioxidant and hepatoprotective potential of CX rhizome. Antioxidant activity of the standardized CX fractions was determined usingin vitroassays. Hepatoprotective assay was conducted against carbon tetrachloride- (CCl4-) induced hepatic damage in rats at doses of 125, 250, and 500 mg/kg of hexane fraction. Highest antioxidant activity was found in hexane fraction. In the case of hepatoprotective activity, CX hexane fraction showed significant improvement in terms of a biochemical liver function, antioxidative liver enzymes, and lipid peroxidation activity. Good recovery was observed in the treated hepatic tissues histologically. Hence, the results concluded that CX hexane fraction possessed prominent hepatoprotective activities which might be due to itsin vitroantioxidant activity. These findings also support the use of CX as a functional food for hepatitis remedy in traditional medicinal system.

scholarly journals Hepatoprotection and Phytochemistry of the Vietnamese Herbs Cleome chelidonii and Cleome viscosa Stems

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Nhat Minh Phan ◽  
Thi Hong Tuoi Do ◽  
Le Thanh Tuyen Nguyen ◽  
Trong Tuan Nguyen ◽  
Quoc Luan Ngo ◽  
...  
Keyword(s):  
Body Weight ◽  
Carbon Tetrachloride ◽  
Alanine Aminotransferase ◽  
Liver Toxicity ◽  
Human Hepatoma ◽  
Human Hepatoma Hepg2 Cells ◽  
Phytochemical Studies ◽  
Hepatoma Hepg2

The study aims to determine the hepatoprotective effect of n-hexane, ethyl acetate, and methanol extracts of the leaves and stems of two Cleome species against carbon tetrachloride- (CCl4-) induced liver toxicity both in vitro using human hepatoma (HepG2) cells and in vivo in rats as well as the hepatoprotective property of all isolated compounds on HepG2. After 72 h of treatment, at the concentrations of 25, 50, and 100 μg/mL, the methanol of C. chelidonii stems (CCSM) ranged from 18.6% to 20.8%, whereas the methanol of C. chelidonii stems (CVSM) increased from 12.3% to 17.2% cell viability. The results show that CCSM and CVSM significantly expressed in vitro hepaprotective activity on HepG2. Therefore, the animals were daily treated with these extracts at the doses of 15, 30, and 45 mg/kg body weight for 5 days, and CCl4 was injected (2 ml/kg body weight, i.p.) on the 2nd and 3rd days. Levels of aspartate aminotransferase (ALT) and alanine aminotransferase (AST) in the blood were measured and compared to the silymarin control. The treatments with CCSM and CVSM (30, and 45 mg/kg) possessed significant hepatoprotection and were comparable with the activity of silymarin. Further, phytochemical studies of these ones were conducted and led to the identification of eight flavonoids: visconoside A (1), visconoside B (2), quercetin 3-O-β-D-glucopyranoside 7-O-α-L-rhamnopyranoside (3), kaempferol 3-O-β-D-glucopyranoside 7-O-α-L-rhamnopyranoside (4), cleomeside A (5), cleomeside B (6), cleomeside C (7), and quercetin-3-O-[β-D-glucopyranosyl-(1⟶2)]-α-L-rhamnopyranoside 7-O-α-L-rhamnopyranoside (8). Two major flavonoids (1 and 4) displayed significant hepatoprotective property (at the concentration of 100 μM, the prevention percentage values were 66.5% and 74.2%, respectively, compared to the quercetin control, with value of 80.3%).

scholarly journals THE EFFECT OF BILIMBI (BELIMBING WULUH) EXTRACT (Averrhoa bilimbi L) AGAINST DENTAL REMINERALIZATION AND ENAMEL MICROSTRUCTURE (IN VITRO RESEARCH)

2017 ◽  
Vol 20 (1) ◽  
pp. 20-26
Author(s):  
Trimurni Abidin ◽  
Marline Nainggolan ◽  
Susi Susi
Keyword(s):  
Tooth Enamel ◽  
Enamel Surface ◽  
Surface Microstructure ◽  
Enamel Microstructure ◽  
Group Iii ◽  
Group I ◽  
High Calcium ◽  
Significant Difference ◽  
The Difference

Teeth physiologically undergo demineralization and remineralisation processes. One way to reduce demineralization can be done with the use of CPP-ACP (Casein Phosphopeptide-Amorphous Calcium Phosphate), which has high calcium bioavailability. The use of natural materials products in the field of dentistry is currently growing, one of which is bilimbi/ starfruit (belimbingwuluh) that is rich in minerals and has many benefits. This study aims to analyze the effect of extract of bilimbi in tooth enamel remineralisation which is tested by using SEM and EDX equipments so that the difference of enamel surface microstructure and the amount of calcium and phosphorus content of tooth enamel surface can be identified. A total of eight hidden molar teeth were extracted, cut into four parts and divided into four groups, namely group I: the teeth applied with the wuluh bilimbi gel, group II: the teeth applied with CPP-ACP gel, group III: the teeth applied with the combination gel of starfruit extract and CPP-ACP gel, group IV: without any application. The result of this study was assessed qualitatively and it was noticeable that the treatment group combination of CPP-ACP gel and starfruit gel had the smoothest enamel surface microstructure. Quantitatively, there were significant difference is the three treatment groups (p≤ 0.05). The conclusion of this research is the effect of the application of wuluh bilimbi extract gel, CPP-ACP gel and gel combination of starfruit with CPP-ACP gel towards the remineralisation and microstructure of tooth enamel surface.

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