scholarly journals Expert opinion on current and emerging treatments in dermatophytosis

2021 ◽  
Vol 8 (1) ◽  
pp. 161
Author(s):  
R. D. Kharkar ◽  
Mukesh Girdhar ◽  
Gautam Banerjee ◽  
T. Salim ◽  
Manas Sen ◽  
...  
Keyword(s):  
Public Health Problem ◽  
Molecular Techniques ◽  
Antifungal Drugs ◽  
Culture Methods ◽  
Patient Counselling ◽  
Diagnosis And Management ◽  
Emerging Treatments ◽  
Oral Itraconazole ◽  
Conventional Microscopy ◽  
Effective Diagnosis

<p class="abstract">Dermatophytosis continues to be a worldwide public health problem, predominantly in developing countries like India. Early diagnosis and appropriate management are imperative to enhance patient outcomes and improve quality of life of individuals with dermatophytosis. Multiple focused group discussions involving 76 dermatologists across 36 cities in India were conducted to provide a consensus clinical viewpoint of expert dermatologists to gain insights toward effective diagnosis and management of Indian subjects with dermatophytosis. These discussions mainly aimed at reviewing current literature on prevalence, etiology, diagnosis and management of dermatophytosis and highlighting variations in diagnostic and treatment approaches in routine clinical practice. The current article summarizes the experts’ clinical viewpoint on overall management of dermatophytosis. Diagnosis of dermatophytosis involves clinical observation, microscopic examination and dermoscopy. Molecular techniques have certain advantages over conventional microscopy and culture methods but are associated with issues of cost and technique complexity. Oral itraconazole 200 mg–400 mg daily and terbinafine 500 mg/day could be considered for recalcitrant tinea infections. Topical azoles like luliconazole, sertaconazole, and terbinafine could be beneficial. A combination of oral and topical antifungal drugs is effective in patients with steroid-modified and difficult-to-treat tinea infections. Also, patient counselling and use of adjunctive therapies like antihistamines, retinoids, and moisturizers are essential for managing tinea infections.  </p><p class="abstract"> </p>

scholarly journals Dissemination of Fluoroquinolone-Resistant Campylobacter spp. within an Integrated Commercial Poultry Production System

2006 ◽  
Vol 72 (5) ◽  
pp. 3441-3447 ◽  
Author(s):  
Umelaalim Idris ◽  
Jingrang Lu ◽  
Marie Maier ◽  
Susan Sanchez ◽  
Charles L. Hofacre ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Molecular Techniques ◽  
Broiler Chicks ◽  
Campylobacter Coli ◽  
Poultry Production ◽  
Broiler Breeders ◽  
Culture Methods ◽  
Specific Pcr ◽  
Commercial Broiler ◽  
Multiple Levels

ABSTRACTWhile characterizing the intestinal bacterial community of broiler chickens, we detected ε-proteobacterial DNA in the ilea of 3-day-old commercial broiler chicks (J. Lu, U. Idris, B. Harmon, C. Hofacre, J. J. Maurer, and M. D. Lee, Appl. Environ. Microbiol.69:6816-6824, 2003). The sequences exhibited high levels of similarity toCampylobacter jejuniandCampylobacter colisequences, suggesting that chickens can carryCampylobacterat a very young age.Campylobactersp. was detected by PCR in all samples collected from the ilea of chicks that were 3 to 49 days old; however, it was detected only in the cecal contents of chickens that were at least 21 days old. In order to determine whether the presence ofCampylobacterDNA in young chicks was due to ingestion of the bacteria in food or water, we obtained commercial broiler hatching eggs, which were incubated in a research facility until the chicks hatched. DNA sequencing of the amplicons resulting fromCampylobacter-specific 16S PCR performed with the ileal, cecal, and yolk contents of the day-of-hatching chicks revealed thatCampylobacterDNA was present before the chicks consumed food or water. The 16S rRNA sequences exhibited 99% similarity toC. jejuniandC. colisequences and 95 to 98% similarity to sequences of other thermophilicCampylobacterspecies, such asC. lariandC. upsaliensis. The presence ofC. coliDNA was detected by specific PCR in the samples from chicks obtained from a commercial hatchery; however, noCampylobacterwas detected by culturing. In order to determine whether the same strains of bacteria were present in multiple levels of the integrator, we culturedCampylobactersp. from a flock of broiler breeders and their 6-week-old progeny that resided on a commercial broiler farm. The broiler breeders had been given fluoroquinolone antibiotics, and we sought to determine whether the same fluoroquinolone-resistant strain was present in their progeny. The isolates were typed by pulsed-field gel electrophoresis, which confirmed that the parental and progeny flocks contained the same strain of fluoroquinolone-resistantC. coli. These data indicate that resistantC. colican be present in multiple levels of an integrated poultry system and demonstrated that molecular techniques or more sensitive culture methods may be necessary to detect early colonization byCampylobacterin broiler chicks.

scholarly journals Rapid Diagnosis of Blood Stream Infections in ICU: Recent Advances

2019 ◽  
Vol 2 (1) ◽  
pp. 177-180
Author(s):  
Raju Prasad Pangeni
Keyword(s):  
Intensive Care ◽  
Diagnostic Tests ◽  
Rapid Detection ◽  
Blood Stream ◽  
Turnover Time ◽  
Rapid Diagnosis ◽  
Molecular Techniques ◽  
Culture Methods ◽  
Blood Stream Infections

Blood stream infections and sepsis are major causes of hospitalization in most intensive care units around the globe, especially in developing countries like Nepal. Although the sepsis guidelines emphasize the role of early institution of appropriate antibiotics, it is practically challenging due to delayed turnover time of currently available diagnostic tests. Modifications in traditional blood culture methods, use of molecular techniques and availability of biomarkers have raised hope in rapid detection of blood stream infections.

scholarly journals Periocular Cutaneous Mucormycosis Caused by Saksenaea erythrospora

2017 ◽  
Vol 14 (04) ◽  
pp. 209-212
Author(s):  
Bunyada Putthirangsiwong ◽  
Pornchai Mahaisavariya ◽  
Weerawan Chokthaweesak ◽  
Dinesh Selva
Keyword(s):  
Complete Recovery ◽  
Molecular Techniques ◽  
First Case ◽  
Chronic Dacryocystitis ◽  
Oral Itraconazole ◽  
Periocular Region ◽  
Cutaneous Mucormycosis ◽  
The Right

Abstract Saksenaea erythrospora is a rare pathogen in humans. Ten adult cases have been previously reported, eight manifested with cutaneous infection, and two presented with invasive rhinosinusitis infection. The authors present a 16-month-old boy with progressive painful mass at the right medial canthus and upper cheek that was unresponsive to broad-spectrum antibiotics. He underwent an anterior orbitotomy and biopsy. Histopathology revealed broad nonseptate sterile hyphae and grew S. erythrospora, which was confirmed by molecular techniques. The patient was treated with intravenous liposomal amphotericin B and oral itraconazole combined with aggressive surgical debridement. The patient made a complete recovery without long-term complications at 4 months of follow-up. Primary cutaneous mucormycosis caused by S. erythrospora may rarely involve the periocular region and mimic chronic dacryocystitis. We report the first case of pediatric periocular cutaneous mucormycosis caused by S. erythrospora.

scholarly journals Bovine tuberculosis at the human-livestock-wildlife interface: Is it a public health problem in Tanzania? A review

2012 ◽  
Vol 79 (2) ◽  
Author(s):  
Bugwesa Z. Katale ◽  
Erasto V. Mbugi ◽  
Sharon Kendal ◽  
Robert D. Fyumagwa ◽  
Gibson S. Kibiki ◽  
...  
Keyword(s):  
Public Health ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Skin Testing ◽  
Public Health Problem ◽  
Molecular Techniques ◽  
Health Concern ◽  
Tuberculin Skin Testing ◽  
Mycobacterium Tuberculosis Infection ◽  
Potential Health

Despite the apparent public health concern about Bovine tuberculosis (BTB) in Tanzania, little has been done regarding the zoonotic importance of the disease and raising awareness of the community to prevent the disease. Bovine tuberculosis is a potential zoonotic disease that can infect a variety of hosts, including humans. The presence of multiple hosts including wild animals, inefficient diagnostic techniques, absence of defined national controls and eradication programs could impede the control of bovine TB. In Tanzania, the diagnosis of Mycobacterium bovis in animals is mostly carried out by tuberculin skin testing, meat inspection in abattoirs and only rarely using bacteriological techniques. The estimated prevalence of BTB in animals in Tanzania varies and ranges across regions from 0.2% to 13.3%, which is likely to be an underestimate if not confirmed by bacteriology or molecular techniques. Mycobacterium bovis has been detected and isolated from different animal species and has been recovered in 10% of apparently healthy wildebeest that did not show lesions at post-mortem. The transmission of the disease from animals to humans can occur directly through the aerosol route and indirectly by consumption of raw milk. This poses an emerging disease threat in the current era of HIV confection in Tanzania and elsewhere. Mycobacterium bovis is one of the causative agents of human extra pulmonary tuberculosis. In Tanzania there was a significant increase (116.6%) of extrapulmonary cases reported between 1995 and 2009, suggesting the possibility of widespread M. bovis and Mycobacterium tuberculosis infection due to general rise of Human Immunodeficiency virus (HIV). This paper aims to review the potential health and economic impact of bovine tuberculosis and challenges to its control in order to safeguard human and animal population in Tanzania.

Qualitative Map of Salmonella Contamination on Young Chicken Carcasses†

2010 ◽  
Vol 73 (9) ◽  
pp. 1596-1603 ◽  
Author(s):  
T. P. OSCAR ◽  
G. K. RUTTO ◽  
J. B. LUDWIG ◽  
S. PARVEEN
Keyword(s):  
Public Health ◽  
Antibiotic Resistance ◽  
Public Health Problem ◽  
Global Public Health ◽  
Culture Methods ◽  
Chi Square ◽  
Conventional Culture ◽  
Chicken Carcass ◽  
Young Chicken ◽  
Chi Square Analysis

Salmonella contamination of poultry is a global public health problem. The objective of this study was to map the distribution of Salmonella on the young chicken carcass, to improve poultry inspection and food safety. Young chickens (n = 70) in the Cornish game hen class were obtained at retail over a 3-year period. Carcasses were aseptically sectioned into 12 parts, and then Salmonella was isolated from whole-part incubations by conventional culture methods. Isolates were characterized for serotype and antibiotic resistance, and by pulsed-field gel electrophoresis (PFGE). Salmonella incidence was 21.5% (181 of 840) for parts and 57.1% (40 of 70) for carcasses. The number of contaminated parts per carcass ranged from 0 to 12, with a mean of 4.5 among contaminated carcasses. Chi-square analysis indicated that Salmonella incidence differed (P &lt; 0.05) among parts, with rib back (38.6%) and sacral back (34.3%) being the most contaminated. Among the 40 contaminated carcasses, there were 37 different patterns of contamination among parts. Of the 33 carcasses with more than one contaminated part, 12.1% contained two serotypes, 33.3% contained two or more antibiotic resistance profiles, and 100% contained two or more PFGE patterns. The most common serotype was Typhimurium (94.5%), and most (97.2%) isolates were resistant to multiple antibiotics. These results indicated a diverse pattern of Salmonella contamination among carcasses and that multiple subtypes of Salmonella were often present on contaminated carcasses. Thus, whole-carcass incubation succeeded by characterization of multiple isolates per carcass is needed to properly assess and manage this risk to public health.

scholarly journals COVID-19-Associated Candidiasis (CAC): An Underestimated Complication in the Absence of Immunological Predispositions?

2020 ◽  
Vol 6 (4) ◽  
pp. 211 ◽  
Author(s):  
Amir Arastehfar ◽  
Agostinho Carvalho ◽  
M. Hong Nguyen ◽  
Mohammad Taghi Hedayati ◽  
Mihai G. Netea ◽  
...  
Keyword(s):  
Fungal Infections ◽  
Antibiotic Use ◽  
Multidrug Resistant ◽  
Molecular Techniques ◽  
Antifungal Drugs ◽  
Diagnostic Tools ◽  
Candida Spp ◽  
Comparative Performance ◽  
Number Of Patients ◽  
Clinical Awareness

The recent global pandemic of COVID-19 has predisposed a relatively high number of patients to acute respiratory distress syndrome (ARDS), which carries a risk of developing super-infections. Candida species are major constituents of the human mycobiome and the main cause of invasive fungal infections, with a high mortality rate. Invasive yeast infections (IYIs) are increasingly recognized as s complication of severe COVID-19. Despite the marked immune dysregulation in COVID-19, no prominent defects have been reported in immune cells that are critically required for immunity to Candida. This suggests that relevant clinical factors, including prolonged ICU stays, central venous catheters, and broad-spectrum antibiotic use, may be key factors causing COVID-19 patients to develop IYIs. Although data on the comparative performance of diagnostic tools are often lacking in COVID-19 patients, a combination of serological and molecular techniques may present a promising option for the identification of IYIs. Clinical awareness and screening are needed, as IYIs are difficult to diagnose, particularly in the setting of severe COVID-19. Echinocandins and azoles are the primary antifungal used to treat IYIs, yet the therapeutic failures exerted by multidrug-resistant Candida spp. such as C. auris and C. glabrata call for the development of new antifungal drugs with novel mechanisms of action.

scholarly journals Antifungal Resistance, Metabolic Routes as Drug Targets, and New Antifungal Agents: An Overview about Endemic Dimorphic Fungi

2017 ◽  
Vol 2017 ◽  
pp. 1-16 ◽  
Author(s):  
Juliana Alves Parente-Rocha ◽  
Alexandre Melo Bailão ◽  
André Correa Amaral ◽  
Carlos Pelleschi Taborda ◽  
Juliano Domiraci Paccez ◽  
...  
Keyword(s):  
Drug Targets ◽  
Antifungal Agents ◽  
Fungal Infections ◽  
Public Health Problem ◽  
Antifungal Drugs ◽  
Fungal Resistance ◽  
Immunocompromised Patients ◽  
Dimorphic Fungi ◽  
Novel Drug ◽  
Novel Drug Targets

Diseases caused by fungi can occur in healthy people, but immunocompromised patients are the major risk group for invasive fungal infections. Cases of fungal resistance and the difficulty of treatment make fungal infections a public health problem. This review explores mechanisms used by fungi to promote fungal resistance, such as the mutation or overexpression of drug targets, efflux and degradation systems, and pleiotropic drug responses. Alternative novel drug targets have been investigated; these include metabolic routes used by fungi during infection, such as trehalose and amino acid metabolism and mitochondrial proteins. An overview of new antifungal agents, including nanostructured antifungals, as well as of repositioning approaches is discussed. Studies focusing on the development of vaccines against antifungal diseases have increased in recent years, as these strategies can be applied in combination with antifungal therapy to prevent posttreatment sequelae. Studies focused on the development of a pan-fungal vaccine and antifungal drugs can improve the treatment of immunocompromised patients and reduce treatment costs.

scholarly journals Diversity and antifungal susceptibility of yeasts isolated by multiple-tube fermentation from three freshwater lakes in Brazil

2009 ◽  
Vol 8 (2) ◽  
pp. 279-289 ◽  
Author(s):  
Luciana R. Brandão ◽  
Adriana O Medeiros ◽  
Mariana C. Duarte ◽  
Anne C. Barbosa ◽  
Carlos A. Rosa
Keyword(s):  
Amphotericin B ◽  
Heterotrophic Bacteria ◽  
Yeast Species ◽  
Antifungal Susceptibility ◽  
Molecular Techniques ◽  
Antifungal Drugs ◽  
Fecal Coliforms ◽  
Southeastern Brazil ◽  
The People ◽  
Enterococcus Spp

The diversity and antifungal resistance of yeasts able to grow at 37°C and the occurrence of bacterial indicators of water quality were studied in three lakes in Southeastern Brazil. The densities of yeasts, Escherichia coli, Enterococcus spp. and Pseudomonasaeruginosa were determined by the multiple-tube fermentation technique, and counts of heterotrophic bacteria were determined using the pour plate method. The yeasts were identified using physiological and molecular techniques and their resistance to amphotericin B, itraconazole and fluconazole was tested. Yeast occurrence was significantly correlated only with the density of fecal coliforms. Candida krusei, C. guilliermondii and C. tropicalis, the most frequently isolated yeast species, are associated with fecal contamination of water by warm-blooded animals. Yeast isolates were most resistant to amphotericin B (21.7%), followed by itraconazole (20%) and then fluconazole (2.8%). In addition to tests for the fecal coliform group, the density of yeasts grown at 37°C could be used as a complementary microbial indicator that aquatic environments contain organic matter of human origin. The incidence of yeast species resistant to three antifungal drugs shows that these microorganisms could pose a health risk to the people who use these lakes for recreation.

scholarly journals How Can the Microbiologist Help in Diagnosing Neonatal Sepsis?

2012 ◽  
Vol 2012 ◽  
pp. 1-14 ◽  
Author(s):  
Michela Paolucci ◽  
Maria Paola Landini ◽  
Vittorio Sambri
Keyword(s):  
Blood Culture ◽  
Neonatal Sepsis ◽  
Gold Standard ◽  
Early Onset ◽  
Late Onset ◽  
Molecular Techniques ◽  
Diagnosis And Treatment ◽  
Diagnosis And Management ◽  
Sepsis Diagnosis ◽  
Expected Outcomes

Neonatal sepsis can be classified into two subtypes depending upon whether the onset of symptoms is before 72 hours of life (early-onset neonatal sepsis—EONS) or later (late-onset neonatal sepsis—LONS). These definitions have contributed greatly to diagnosis and treatment by identifying which microorganisms are likely to be responsible for sepsis during these periods and the expected outcomes of infection. This paper focuses on the tools that microbiologist can offer to diagnose and eventually prevent neonatal sepsis. Here, we discuss the advantages and limitation of the blood culture, the actual gold standard for sepsis diagnosis. In addition, we examine the utility of molecular techniques in the diagnosis and management of neonatal sepsis.

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