scholarly journals Changes in antioxidant status of heart muscle tissue in experimental diabetes in rabbits.

2002 ◽  
Vol 49 (2) ◽  
pp. 529-535 ◽  
Author(s):  
Anna Gumieniczek ◽  
Hanna Hopkała ◽  
Zbigniew Wójtowicz ◽  
Justyna Nikołajuk
Keyword(s):  
Oxidative Stress ◽  
Defense Mechanisms ◽  
Reductase Activity ◽  
Control Level ◽  
Heart Tissue ◽  
Control Groups ◽  
Time Intervals ◽  
Glutathione Reductase Activity ◽  
And Control ◽  
Sulfhydryl Compounds

The present study was designed to evaluate the oxidative stress-related parameters in alloxan-induced diabetes in rabbits. After 3, 6, 12 and 24 weeks of hyperglycaemia the enzymatic and non-enzymatic factors were measured in heart tissue of diabetic and control groups. Superoxide dismutase and glutathione peroxidase activities and the contents of total sulfhydryl compounds significantly increased at all time intervals. Catalase activity increased initially (after 3 and 6 weeks), decreased after 12 weeks and increased again at the 24th week of the experiment. Glutathione reductase activity increased initially (at 3rd week), decreased below control level after 6 and 12 weeks, then increased again. Ascorbic acid concentration decreased after 3 and 6 weeks, and increased at the 12th and 24th weeks. The level of lipid peroxidation products was reduced after 3, 6 and 12 weeks of the experiment. After 24 weeks it was significantly elevated. These data suggest that hyperglycaemia induces oxidative stress in the heart but the defense mechanisms in the heart tissue are fairly efficacious against oxidative injury.

scholarly journals Ischemia-modified albumin (IMA) and dynamic thiol-disulfide homeostasis in patients with postherpetic neuralgia

2019 ◽  
Vol 43 (5) ◽  
pp. 257-263
Author(s):  
Sule Arican ◽  
Gulcin Hacibeyoglu ◽  
Sinan Oguzhan Ulukaya ◽  
Gamze Avcioglu ◽  
Ruhiye Reisli ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Postherpetic Neuralgia ◽  
Defense Mechanisms ◽  
Healthy Controls ◽  
Control Groups ◽  
Ischemia Modified Albumin ◽  
Total Thiol ◽  
Cobalt Binding ◽  
And Control ◽  
Plasma Thiols

Abstract Background Ischemia-modified albumin (IMA) is an isotype of albumin that increases under oxidative stress, and plasma thiols are main defense mechanisms against oxidative stress. The objective of this study was to investigate thiol-disulfide homeostasis and serum IMA levels in postherpetic neuralgia (PHN) patients. Methods A total of 29 PHN patients and 30 healthy controls were included in the study. Serum total and native thiol concentrations and serum disulfide concentration were measured using the method described by Erel and Neselioglu. The albumin cobalt binding test was used to measure serum IMA levels. Results Serum IMA levels were 1.21 ± 0.58 AU and 0.75 ± 0.09 AU in the PHN and control groups, respectively (p < 0.001). Serum total thiol concentrations were found to be 421.62 ± 90.28 μmol/L and 598.36 ± 73.63 μmol/L in the PHN and control groups, respectively (p < 0.001). Serum native thiol concentrations were found to be 365.75 ± 92.07 μmol/L and 531.90 ± 72.9 μmol/L in the PHN and control groups, respectively (p < 0.001). Serum disulfide concentrations were found to be 33.23 ± 5.33 μmol/L and 27.93 ± 7.81 μmol/L in the PHN and control groups, respectively (p = 0.003). The native thiol/total thiol ratio was significantly lower, and the disulfide/total thiol and disulfide/native thiol ratios were significantly higher in the PHN group compared to the controls. Conclusions IMA levels are high and dynamic thiol/disulfide homeostasis is disrupted in PHN patients.

scholarly journals In Vivo Bioavailability of Selenium in Selenium-Enriched Streptococcus thermophilus and Enterococcus faecium in CD IGS Rats

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 463
Author(s):  
Gabriela Krausova ◽  
Antonin Kana ◽  
Marek Vecka ◽  
Ivana Hyrslova ◽  
Barbora Stankova ◽  
...  
Keyword(s):  
Glutathione Reductase ◽  
Enterococcus Faecium ◽  
Reductase Activity ◽  
Streptococcus Thermophilus ◽  
Heart Tissue ◽  
Sprague Dawley ◽  
Glutathione Reductase Activity ◽  
Novel Concept ◽  
The Individual

The selenium (Se) enrichment of yeasts and lactic acid bacteria (LAB) has recently emerged as a novel concept; the individual health effects of these beneficial microorganisms are combined by supplying the essential micronutrient Se in a more bioavailable and less toxic form. This study investigated the bioavailability of Se in the strains Enterococcus faecium CCDM 922A (EF) and Streptococcus thermophilus CCDM 144 (ST) and their respective Se-enriched forms, SeEF and SeST, in a CD (SD-Sprague Dawley) IGS rat model. Se-enriched LAB administration resulted in higher Se concentrations in the liver and kidneys of rats, where selenocystine was the prevalent Se species. The administration of both Se-enriched strains improved the antioxidant status of the animals. The effect of the diet was more pronounced in the heart tissue, where a lower glutathione reductase content was observed, irrespective of the Se fortification in LAB. Interestingly, rats fed diets with EF and SeEF had higher glutathione reductase activity. Reduced concentrations of serum malondialdehyde were noted following Se supplementation. Diets containing Se-enriched strains showed no macroscopic effects on the liver, kidneys, heart, and brain and had no apparent influence on the basic parameters of the lipid metabolism. Both the strains tested herein showed potential for further applications as promising sources of organically bound Se and Se nanoparticles.

scholarly journals The effect of diminazene aceturate on cholinesterase activity in dogs with canine babesiosis

1997 ◽  
Vol 68 (4) ◽  
Author(s):  
R.J. Milner ◽  
F. Reyers ◽  
J.H. Taylor ◽  
J.S. Van den Berg
Keyword(s):  
Treatment Group ◽  
Underlying Mechanism ◽  
Time Interval ◽  
Control Groups ◽  
Canine Babesiosis ◽  
Diminazene Aceturate ◽  
Time Intervals ◽  
Significant Depression ◽  
The Difference ◽  
And Control

A clinical trial was designed to evaluate the effects of diminazene aceturate and its stabiliser antipyrine on serum pseudocholinesterase (PChE) and red blood cell acetylcholinesterase (RBC AChE) in dogs with babesiosis. The trial was conducted on naturally occurring, uncomplicated cases of babesiosis (n = 20) that were randomly allocated to groups receiving a standard therapeutic dose of diminazene aceturate with antipyrine stabiliser (n = 10) or antipyrine alone (n = 10). Blood was drawn immediately before and every 15 minutes for 1 hour after treatment. Plasma PChE showed a 4 % decrease between 0 and 60 min within the treatment group (p < 0.05). No statistically significant differences were found between the treatment and control groups at any of the time intervals for PChE. There was an increase in RBC AChE activity at 15 min in the treatment group (p < 0.05). No significant differences were found between the treatment and control groups at any time interval for RBC AChE. In view of the difference in PChE, samples from additional, new cases (n = 10) of canine babesiosis were collected to identify the affect of the drug over 12 hours. No significant depression was identified over this time interval. The results suggests that the underlying mechanism in producing side-effects, when they do occur, is unlikely to be through cholinesterase depression.

scholarly journals Experimental Pulpitis Treated By Double-Pinhole Drainage

2019 ◽  
Vol 3 (2) ◽  
Keyword(s):  
Dental Pulp ◽  
Control Group ◽  
Pulp Chamber ◽  
Control Groups ◽  
Time Intervals ◽  
Irrigation Methods ◽  
Significant Difference ◽  
And Control ◽  
Experimental Group ◽  
Labial Surface

Objective: To search for a dental pulp preservative method in experimental pulpitis. Methods: 20 rabbits with experimental pulpitis were selected for this study; Teeth were randomly assigned to 2 groups (experimental and control groups) based on the final irrigation methods. In experimental group (double-pinhole drainage), the two pinholes were drilled in middle 1/3 dental cervix of buccal labial surface of tooth; the pinholes were inserted into infant transfusion needles then the two syringes were contacted with them, one for suck, another for irrigation. In control group, pinhole was sealed. Rabbits were executed in 1, 3, 5, 7 day. The inflammation in pulp chamber was assessed after 1, 3, 5, and 7 days. Results: At the 1, 5 day time intervals, control group reported more inflammation than experimental group (P < .05). There was no significant difference between two groups at the other time intervals (P > .05), and in all groups the inflammation decreased over time. Conclusion: Dental pulp can be preserved by double-pinhole drainage efficiently.

scholarly journals Oxidative stress and outcome of antioxidant supplementation in patients with polycystic ovarian syndrome (PCOS)

2018 ◽  
Vol 7 (5) ◽  
pp. 1667 ◽  
Author(s):  
Abubakar A. Panti ◽  
Constance E. Shehu ◽  
Yusuf Saidu ◽  
Karima A. Tunau ◽  
Emmanuel I. Nwobodo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Serum Levels ◽  
Clinical Pregnancy ◽  
Oxidative Stress Marker ◽  
Randomised Control Trial ◽  
Antioxidant Supplementation ◽  
Control Groups ◽  
Stress Marker ◽  
And Control

Background: PCOS is a condition with significant decrease in antioxidant with an increased risk of oxidative stress. Antioxidant supplementation has been shown to improve insulin sensitivity in PCOS and may improve outcome of management of PCOS. Objectives of this study were to determine the oxidative stress level of PCOS patients, to assess the effect of antioxidant supplementation on the outcome of management of PCOS and to compare with a control group.Methods: The study was a single blind randomised control trial involving 200 patients with PCOS. The study was conducted at Usmanu Danfodiyo University Teaching Hospital Sokoto. They were randomly divided into intervention and control groups and base line serum levels of oxidative stress marker, antioxidant enzymes, vitamins and minerals were determined. Antioxidant supplementation and placebo were given to the intervention and control groups respectively. All the patients had ovulation induction with clomiphene citrate and were followed up for 6 months. Outcome measures were clinical pregnancy or menstrual regularisation. Level of significance was <0.05.Results: There was statistical significance in the serum levels of oxidative stress marker, antioxidant enzymes, vitamins and minerals between the two groups (post intervention). Glutathione peroxidase (p = 0.001), superoxide dismutase (p = 0.0001), catalase (p= 0.0369), melondialdehyde (p= 0.007), Vitamin A, Vitamin C, Vitamin E (p = 0.0001), zinc and copper (p = 0.0001). The clinical pregnancy outcomes were 22 (22%) versus 2 (2%); live births 18 (18%) versus 2 (2%) and menstrual regularisation 48 (48%) versus 46 (46%) in the intervention and control groups respectively.Conclusions: Antioxidant supplementation in this study significantly affected pregnancy rate in patients with PCOS. Larger studies are suggested to revisit the conclusion of the Cochrane review that antioxidants supplementation had no significant role in female infertility.

scholarly journals The Preventive Effects of Asparagus officinalis Extract on Sodium Selenite-Induced Cataractogenesis in Experimental Animal Models

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Momammad Azadbakht ◽  
Mohammad Asghari ◽  
Kiumars Nowroozpoor Dailami ◽  
Ali Davoodi ◽  
Amirhossein Ahmadi
Keyword(s):  
Sodium Selenite ◽  
Reductase Activity ◽  
Control Plant ◽  
Asparagus Officinalis ◽  
Safe Alternative ◽  
Hydroalcoholic Extract ◽  
Plant Groups ◽  
Malondialdehyde Content ◽  
Glutathione Reductase Activity ◽  
And Control

Background and Objectives. Cataract is the leading cause of blindness worldwide. Although surgery is now considered the most successful cure, the development of alternative treatments is needed due to postsurgical complications. Oxidative stress in the lens is considered to be the most crucial factor in the formation of cataracts. Therefore, the effects of the hydroalcoholic extract of Asparagus officinalis L, a traditional antioxidative plant, on cataract formation of sodium selenite were evaluated. Materials and Methods. Neonatal rats received a single dose of sodium selenite as an intraperitoneal injection (30 μmol/kg) on day 10 postnatal to induce cataract. Animals were then posttreated with various oral solutions of A. officinalis extract at 200 mg/kg or 400 mg/kg once daily on days 10–16 postnatal. Cataract was evaluated by slit-lamp, and lens opacification was analyzed in each group 24 hours after the last treatment at day seven postadministration of the extracts or vehicle. The total protein concentration of lenses, glutathione reductase activity as the glutathione antioxidant capacity, and malondialdehyde content as a marker of lipid peroxidation were further assessed in removed rat lenses on day 30 postnatal. Results. All lenses in the healthy and control plant groups were clear. Sodium selenite significantly increased cataract grade (2.8 ± 0.2) when compared to the healthy group p = 0.001 . However, cataract grades were decreased considerably as 1.9 ± 0.72 and 1.5 ± 0.85 in groups that received 200 mg/kg and 400 mg/kg oral extract of A. officinalis, respectively. A. officinalis extract also restored all abnormalities of biochemical markers induced by sodium selenite. Conclusion. Our data suggest that A. officinalis could be a promising candidate as a safe alternative treatment in cataracts upon further clinical trials. This effect is probably associated with the antioxidant activity of A. officinalis.

scholarly journals Effects of Myosmine on Antioxidative Defence in Rat Liver

2012 ◽  
Vol 63 (1) ◽  
pp. 7-14 ◽  
Author(s):  
Rumyana Simeonova ◽  
Vessela Vitcheva ◽  
Galina Gorneva ◽  
Mitka Mitcheva
Keyword(s):  
Oxidative Stress ◽  
Rat Liver ◽  
Wistar Rats ◽  
Reduced Glutathione ◽  
Reductase Activity ◽  
Hepatic Function ◽  
Positive Control ◽  
The Other ◽  
Antioxidative Defence ◽  
Glutathione Reductase Activity

Effects of Myosmine on Antioxidative Defence in Rat LiverMyosmine [3-(1-pyrrolin-2-yl) pyridine] is an alkaloid structurally similar to nicotine, which is known to induce oxidative stress. In this study we investigated the effects of myosmine on enzymatic and non-enzymatic antioxidative defence in rat liver. Wistar rats received a single i.p. injection of 19 mg kg-1 of myosmine and an oral dose of 190 mg kg-1 by gavage. Nicotine was used as a positive control. Through either route of administration, myosmine altered the hepatic function by decreasing the levels of reduced glutathione, superoxide dismutase, and glutathione peroxidase activities on one hand and by increasing malondialdehyde, catalase, and glutathione reductase activity on the other. Compared to control, both routes caused significant lipid peroxidation in the liver and altered hepatic enzymatic and non-enzymatic antioxidative defences. The pro-oxidant effects of myosmine were comparable with those of nicotine.

Analysis of Glutathione-Dependent Enzyme Activities in two Different Rat Hepatomas and in Normal Liver in Relation to their Role in Resistance to Oxidative Stress

1988 ◽  
Vol 74 (6) ◽  
pp. 617-621 ◽  
Author(s):  
Maria Armida Rossi ◽  
Mario Umberto Dianzani
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Reductase Activity ◽  
Antioxidant Properties ◽  
Liver Microsomes ◽  
Normal Liver ◽  
Lipid Hydroperoxides ◽  
Glutathione S Transferases ◽  
Glutathione Reductase Activity

The importance of some glutathione metabolic pathways was examined in two highly dedifferentiated hepatomas, Yoshida AH-130 and Morris 3924 A hepatomas, and in normal liver in relation to their role against oxidative stress. The cytosol prepared from Yoshida hepatoma cells decreased the peroxidation rate in normal liver microsomes and mitochondria, but this antioxidant property was not displayed by Morris hepatoma. Glutathione peroxidase and glutathione-S-transferases activities were extremely low in both hepatomas; glutathione reductase activity values were about half the normal liver values. The large decrease in glutathione peroxidase and glutathione-S-transferases suggests that in these two tumors only small amounts of GSH can be used in reduction or conjugation reactions, such as the reduction of hydrogen peroxide and lipid hydroperoxides or the conjugation of GSH with the end products of lipoperoxidation, aldehydes or ketones. The hypothesis of a more efficient GSSG reduction in hepatomas, due to the low glutathione peroxidase/glutathione reductase activity ratio, is also discussed. The described changes in glutathione related enzymes do not seem to have any correlation with the protective effect against the lipoperoxidative processes displayed by some tumors since these enzymatic activities were similar in both hepatomas whereas only Yoshida hepatoma showed antioxidant properties.

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