THE EFFICACY OF PLATELET GROWTH FACTORS (PRP) IN THE COMPLEX TREATMENT OF TMJ  MALFUNCTIONS

Background. Joint pain is often associated not only with disc displacement but also due to the level of inflammatory mediators in the synovial fluid. Injections of platelet growth factor not only expand the joint cavity, performing a purely mechanical function but also create a high concentration of growth factor in the synovial fluid, which favorably affects the cartilage tissue, joint capsule, ligamentous apparatus. Objectives ― to study the effectiveness of platelet growth factor in the complex treatment of patients with TMJ malfunctions. Methods. A clinical examination of two groups of patients (50 people) aged 18 to 67 years with a malfunctions of TMJ was conducted. Were used in all patients in the complex: splint therapy, myogymnastics, and manual traction drive. For the first group (25 people), concentrated growth factor in the liquid phase (PRP) was used in the treatment. For the second group (PRP) was not used. The patients were divided into two groups randomly. Results. The results of the study were carried out according to the following criteria: pain in TMJ with a maximum opening of the mouth, protrusion, laterotruzii, sound phenomena in TMJ, myofascial pain (palpation), the presence of deviation/deflection of the lower jaw, incisional distance. Conclusions. 1. Complex therapy, including splint therapy, myogymnastics, manual traction of the disc, the use of platelet growth factor, showed good results in the treatment of TMJ malfunctions. 2. According to all the studied criteria (pain in TMJ with a maximum opening of the mouth, protrusion, laterotruzii, sound phenomena in TMJ, myofascial pain (palpation), the presence of deviation/deflection of the lower jaw), the most effective result of treatment was obtained after 1 month.

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Evaluation of bone formation capacities of human adipose-derived stromal cells cultured in platelet growth factor-enriched plasma medium.

Bone Abstracts ◽

10.1530/boneabs.1.pp182 ◽

2013 ◽

Author(s):

Fabien Guilloton ◽

Vahideh Rabani ◽

Meadhbh Brennan ◽

Giulio Bassi ◽

Mauro Krampera ◽

...

Keyword(s):

Growth Factor ◽

Bone Formation ◽

Stromal Cells ◽

Plasma Medium ◽

Adipose Derived Stromal Cells ◽

Platelet Growth Factor ◽

Cells Cultured

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Cell Source-Dependent In Vitro Chondrogenic Differentiation Potential of Mesenchymal Stem Cell Established from Bone Marrow and Synovial Fluid of Camelus dromedarius

Animals ◽

10.3390/ani11071918 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1918

Author(s):

Young-Bum Son ◽

Yeon Ik Jeong ◽

Yeon Woo Jeong ◽

Mohammad Shamim Hossein ◽

Per Olof Olsson ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Stem Cell ◽

Synovial Fluid ◽

Cartilage Regeneration ◽

Cartilage Tissue ◽

Chondrocyte Differentiation ◽

Differentiation Potential ◽

Proliferation Capacity

Mesenchymal stem cells (MSCs) are promising multipotent cells with applications for cartilage tissue regeneration in stem cell-based therapies. In cartilage regeneration, both bone marrow (BM-MSCs) and synovial fluid (SF-MSCs) are valuable sources. However, the cellular characteristics and chondrocyte differentiation potential were not reported in either of the camel stem cells. The in vitro chondrocyte differentiation competence of MSCs, from (BM and SF) sources of the same Camelus dromedaries (camel) donor, was determined. Both MSCs were evaluated on pluripotent markers and proliferation capacity. After passage three, both MSCs showed fibroblast-like morphology. The proliferation capacity was significantly increased in SF-MSCs compared to BM-MSCs. Furthermore, SF-MSCs showed an enhanced expression of transcription factors than BM-MSCs. SF-MSCs exhibited lower differentiation potential toward adipocytes than BM-MSCs. However, the osteoblast differentiation potential was similar in MSCs from both sources. Chondrogenic pellets obtained from SF-MSCs revealed higher levels of chondrocyte-specific markers than those from BM-MSCs. Additionally, glycosaminoglycan (GAG) content was elevated in SF-MSCs related to BM-MSCs. This is, to our knowledge, the first study to establish BM-MSCs and SF-MSCs from the same donor and to demonstrate in vitro differentiation potential into chondrocytes in camels.

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1. An Oral Platelet Growth Factor

Nihon Naika Gakkai Zasshi ◽

10.2169/naika.98.1662 ◽

2009 ◽

Vol 98 (7) ◽

pp. 1662-1668

Author(s):

Koji Miyazaki

Keyword(s):

Growth Factor ◽

Platelet Growth Factor

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Effects of Passaging on the Migration Response of Synovium-Derived Stem Cells to an Applied DC Electric Field

ASME 2011 Summer Bioengineering Conference, Parts A and B ◽

10.1115/sbc2011-53674 ◽

2011 ◽

Author(s):

Andrea R. Tan ◽

Elena Alegre-Aguarón ◽

Divya N. Dujari ◽

Sonal R. Sampat ◽

J. Chloë Bulinski ◽

...

Keyword(s):

Stem Cells ◽

Growth Factor ◽

Electric Field ◽

Cartilage Tissue Engineering ◽

Joint Space ◽

Cartilage Tissue ◽

Cartilage Defects ◽

Cell Sources ◽

Dc Electric Field ◽

Migration Response

Strategies for cartilage tissue engineering and repair have recently focused on cell sources from the surrounding joint tissue as an alternative to chondrocytes. Synovium-derived stem cells (SDSCs) are found in the intimal layer of the synovium, the thin overlying capsule surrounding the joint space [1] and have been found to exhibit a greater chondrogenic potential than stem cells from other origins such as bone marrow stem cells or adipose derived stem cells [2–4]. Under directed cues, these cells have been shown to be capable of migrating from the synovium membrane into articular cartilage defects, though the mechanism behind such movement is unclear. As a first step, we have previously shown that SDSCs expanded in 2D monolayer culture in a growth factor cocktail of TGF-β1, FGF, and PDGF-ββ exhibit directed cathodal migration with perpendicular alignment when under the influence of an applied DC electric field [5]. As cellular behavior and response to an external stimulus can change with exposure to growth factors and passage number, we look here to characterize the effects of passaging on the migration response of SDSCs to an applied electric field. We hypothesize that if these cells develop more chondrocyte-like characteristics with growth factor passaging, their response will mimic that which has previously been reported for chondrocytes, notably directed cathodal (negative pole) migration and perpendicular realignment of the long axis to the direction of applied field [6].

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CTRP9 protects against MIA-induced inflammation and knee cartilage damage by deactivating the MAPK/NF-κB pathway in rats with osteoarthritis

Open Life Sciences ◽

10.1515/biol-2020-0105 ◽

2020 ◽

Vol 15 (1) ◽

pp. 971-980

Author(s):

Shicheng Zheng ◽

Jing Ren ◽

Sihai Gong ◽

Feng Qiao ◽

Jinlong He

Keyword(s):

Synovial Fluid ◽

Nuclear Factor Kappa B ◽

Cartilage Damage ◽

Cartilage Tissue ◽

Enzyme Linked Immunosorbent Assay ◽

Nuclear Factor ◽

Knee Cartilage ◽

Monosodium Iodoacetate ◽

Dose Dependent ◽

Different Doses

AbstractC1q/TNF-related protein 9 (CTRP9), the closest paralog of adiponectin, has been reported to protect against inflammation-related diseases. However, its role in regulating osteoarthritis (OA) has not been fully elucidated. First, a rat model of OA was generated. Furthermore, rats with OA were injected with different doses of recombinant CTRP9 protein (rCTRP9), and the knee cartilage damage was evaluated. Finally, the phosphorylation of p38 and the secretion of matrix metalloproteinases (MMPs) were detected by Western blotting and enzyme-linked immunosorbent assay. Results revealed that CTRP9 was highly expressed in adipose tissue, followed by skeletal muscle and cartilage tissue, and less expressed in liver, kidney and lung. Moreover, the expression of CTRP9 significantly decreased in the monosodium iodoacetate (MIA) group in the knee cartilage and knee synovial fluid, and the contents of interleukin-1β (IL-1β) and IL-6 significantly increased in knee synovial fluid. In addition, rCTRP9 alleviated MIA-induced inflammation, oxidative stress and knee cartilage damage in a dose-dependent way. In addition, rCTRP9 could attenuate the expression of p38MAPK and p-p38 and suppress the expression of nuclear factor-kappa B (NF-κB), p65 and MMPs. Collectively, the results of the present study suggested that CTRP9 alleviates the inflammation of MIA-induced OA through deactivating p38MAPK and NF-κB signaling pathways in rats.

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Research on the Effect of p35 for Secretion of Proinflammatory Factor and Apoptosis of Chondrocyte in Chondrocytopathic Articular Fluid

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2021.2621 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1649-1654

Author(s):

LvLin Yang ◽

Bowen Zhang ◽

Yuqi Liang ◽

Gangning Feng ◽

Xiaoke Shang ◽

...

Keyword(s):

Transcriptional Regulation ◽

Synovial Fluid ◽

Intervention Group ◽

Inflammatory Cells ◽

Cartilage Tissue ◽

Control Group ◽

Inflammatory Factor ◽

Proinflammatory Factor ◽

Group B ◽

Group Control Group

To study on the effect of transcriptional regulation factor as p35 for secretion of proinflammatory factor and apoptosis of chondrocyte in chondrocytopathic articular fluid so as to improve the chondropathy. The fifty SD rats were selected for our study. It was divided into three groups including A group (control group), B group (chondrocytopathic model group of osteoarthritis) and C group (transcriptional regulation factor as p35 intervention group. The samples were collected after intervention in sixteen weeks. The sampling position was cartilage tissue of rat leg. It was adopted for immunohistochemical inspection and histopathology examination. At the same time the synovial fluid was collected. The concentration of TNF-α and IL-6 was detected. And the expression of mRNA in gene related with apoptosis was detected. The chondrocyte morphology of rats in A group was normal. The chondrocyte was damaged and goblet cell was reduced in B group. The infiltrating inflammatory cells in C group were less than in B group from pathological results. And the goblet cells in C group was increased than in B group. The expression of TNF-α, Bax, NF-κB, IL-6: B group > C group > A group. The expression of Bcl-2: A group > C group > B group. The transcriptional regulation factor as p35 related with anti-apoptosis could regulate the level of inflammatory factor as TNF-α and IL-6 in synovial fluid and restrain the lesion and apoptosis of chondrocyte.

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