scholarly journals Effect of 8 Weeks of Incremental Endurance Training on Antioxidant Enzymes and Total Antioxidant Status of Cardiac Tissue in Experimental Diabetic Rats

2020 ◽  
Author(s):  
Eftekhar Mohammadi ◽  
Fatemeh Nikseresht
Keyword(s):  
Antioxidant Enzymes ◽  
Endurance Training ◽  
Antioxidant Status ◽  
Cardiac Tissue ◽  
Total Antioxidant Status ◽  
Diabetic Control ◽  
Heart Tissue ◽  
Diabetic Mice ◽  
Total Antioxidant ◽  
Healthy Control

Introduction: Evidence suggests increased oxidative stress and improved total antioxidant status resulting from regular exercise in the heart tissue of diabetic patients. The aim of this study was to investigate the effect of 8 weeks of increasing endurance training on the levels of antioxidant enzymes and the total antioxidant status of the cardiac tissue of diabetic mice. Methods: In this experimental study, 24 Wistar male rats (aged 10 weeks and weighing 256±11.8 g) were divided into 4 groups of 6.The training program lasted for 8 weeks with increasing endurance training. It was measured 48 hours after the completion of the protocol levels of antioxidant enzymes (catalase, glutathione, and glutathione peroxidase) and the total antioxidant status of rat heart tissue. One-way analysis of variance test was used for intergroup comparisons and Pearson test was used to investigate the relationship between indicators. Results: The results showed a significant difference in total antioxidant status, catalase and glutathione (P = 0.001) and were not significant in glutathione peroxidase (P = 0.240). Moreover, a follow-up test showed a significant increase in total antioxidant status and catalase (healthy exercise, healthy control, and diabetic exercise compared to diabetic control), Glutathione (healthy exercise (P = 0.001) and healthy control (P = 0.049) compared to diabetic control); (Healthy exercise (P = 0.003) compared to diabetic exercise. Finally, some correlations between antioxidant enzymes and total antioxidant status were significant. Conclusion: According to research results, increasing endurance training appears to increase levels of antioxidant enzymes and improve total antioxidant status, thereby it reduces oxidative stress in the heart tissue of diabetic mice.

scholarly journals Effect of Eight Weeks of Endurance Training and Stevia Supplemention on Atrial Natriuretic Peptide and β-Myosin Heavy Chain Expression Levels in Heart Tissue of Rats With Type 1 Diabetes

2020 ◽  
Vol 24 (4) ◽  
pp. 308-319
Author(s):  
Kobra Soleymani ◽  
◽  
Asieh Abbassi Daloii ◽  
Ali Reza Barari ◽  
Ayoub Saeidi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endurance Training ◽  
Cardiac Tissue ◽  
Diabetic Control ◽  
Heart Tissue ◽  
Control Group ◽  
Expression Levels ◽  
Diabetic Control Group ◽  
Healthy Control

Background: The effects of exercise and stevia extract on diabetes-related indicators have been already reported, but their cardiac benefits on Type 1 Diabetes (T1D) are unclear. Objective: This study aims to evaluate the effect of eight weeks of endurance training and stevia supplementation on gene expression levels of Atrial Natriuretic Peptide (ANP) and β-Myosin Heavy Chain (β-MHC) in the heart tissue of T1D rats. Methods: In this experimental study, 25 rats with the average weight of 250-300 g were divided into five groups; healthy control, diabetic control, diabetic+supplementation, diabetic+training, and diabetic+training+supplementation. T1D was induced by intraperitoneal injection of streptozotocin (55 mg/kg/ body weight). Endurance training was performed 5 days a week at a speed of 20-30 meters per minute on a surface with a zero slope for 8 weeks. Stevia was gavaged in a dose of 250 mg/kg/body weight. Rats were slaughtered 48 hours after the last training session. Cardiac tissue was used to measure the parameters. The gene expression of ANP and b-MHC in cardiac tissue was measured by real-time Polymerase Chain Reaction (PCR) method. Data were analyzed by using one-way ANOVA and Bonferroni post hoc test. Findings: The gene expression levels of ANP and β-MHC were significantly higher in the diabetic control group compared to the healthy control group (P=0.001), and significantly lower in the diabetic+training and diabetic+training+supplementation groups compared to the diabetic control group (P=0.001). Conclusion: Endurance training and stevia supplementation can have beneficial effects on the heart of T1D rats.

scholarly journals How do glutathione antioxidant enzymes and total antioxidant status respond to air pollution exposure?

2018 ◽  
Vol 112 ◽  
pp. 287-293 ◽  
Author(s):  
Zeinab Farhat ◽  
Richard W. Browne ◽  
Matthew R. Bonner ◽  
Lili Tian ◽  
Furong Deng ◽  
...  
Keyword(s):  
Air Pollution ◽  
Antioxidant Enzymes ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Air Pollution Exposure ◽  
Total Antioxidant ◽  
Pollution Exposure

scholarly journals The antioxidant defense system of Varroa destructor mites facilitates the infestation of Apis mellifera

2016 ◽  
Vol 60 (1) ◽  
pp. 163-166
Author(s):  
Kamila Dmochowska-Ślęzak ◽  
Małgorzata Dmitryjuk ◽  
Ewa Zaobidna ◽  
Krystyna Żółtowska
Keyword(s):  
Antioxidant Enzymes ◽  
Varroa Destructor ◽  
Antioxidant Status ◽  
Antioxidant Defense ◽  
Total Antioxidant Status ◽  
Antioxidant Defense System ◽  
Low Molecular Weight ◽  
Defense System ◽  
Total Antioxidant ◽  
Parasitic Mite

Abstract Varroa destructor is a parasitic mite of the Western honey bee. The activity of five antioxidant enzymes of V. destructor were analysed. Glutathione content and total antioxidant status was also evaluated. Our results suggest that antioxidant enzymes constitute the main line of defense against ROS in V. destructor, whereas low-molecular-weight antioxidants play a limited role in the antioxidant system of mites.

scholarly journals Relationship between Estradiol and Antioxidant Enzymes Activity of Ischemic Stroke

2009 ◽  
Vol 2009 ◽  
pp. 1-5 ◽  
Author(s):  
Nasrin Sheikh ◽  
Heidar Tavilani ◽  
Aliakbar Rezaie ◽  
Asad Vaisi-raygani ◽  
Saeedeh Salimi
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Ischemic Stroke ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Control Group ◽  
Significant Difference ◽  
Total Antioxidant ◽  
And Control

Some evidence suggests the neuroprotection of estrogen provided by the antioxidant activity of this compound. The main objective of this study was to determine the level of estradiol and its correlation with the activity of antioxidant enzymes, total antioxidant status and ferritin from ischemic stroke subjects. The study population consisted of 30 patients with acute ischemic stroke and 30 controls. There was no significant difference between estradiol in stroke and control group. The activity of superoxide dismutase and level of ferritin was higher in stroke compared with control group (P<.05,P<.001, resp.). There was no significant correlation between estradiol and glutathione peroxidase, glutathione reductase, catalase, total antioxidant status, and ferritin in stroke and control groups. We observed inverse correlation between estradiol with superoxide dismutase in males of stroke patients (r=−0.54,P=.029). Our results supported that endogenous estradiol of elderly men and women of stroke or control group has no antioxidant activity.

scholarly journals Association of Age-Related Macular Degeneration with Erythrocyte Antioxidant Enzymes Activity and Serum Total Antioxidant Status

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Ivna Plestina-Borjan ◽  
Damir Katusic ◽  
Maria Medvidovic-Grubisic ◽  
Daniela Supe-Domic ◽  
Kajo Bucan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Macular Degeneration ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Age Related Macular Degeneration ◽  
Enzymes Activity ◽  
Age Related ◽  
Total Antioxidant ◽  
Gpx Activity

The aim was to estimate association of the oxidative stress with the occurrence of age-related macular degeneration (AMD). The activities of erythrocyte antioxidant enzymes: superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) and additionally serum total antioxidant status (TAS) were used as indicators of the oxidative stress level. 57 AMD patients (32 early and 25 late AMD) and 50 healthy, age and gender matched controls were included. GPx activity (P<0.001) and serum TAS (P=0.015) were significantly lower in AMD patients. The difference was not significant for SOD or CAT activities. Significant interaction between GPx and SOD was detected (P=0.003). At high levels of SOD activity (over 75th percentile), one standard deviation decrease in GPx increases the odds for AMD for six times (OR = 6.22;P<0.001). ROC analysis revealed that combined values of GPx activity and TAS are significant determinants of AMD status. Accuracy, sensitivity, specificity, and positive and negative predictive values were 75%, 95%, 52%, 69%, and 90%, respectively. The study showed that low GPx activity and TAS are associated with AMD. SOD modulates the association of GPx and AMD. The results suggest that erythrocyte antioxidant enzymes activity and serum TAS could be promising markers for the prediction of AMD.

Association between calcitriol and paricalcitol with oxidative stress in patients with hemodialysis

2020 ◽  
pp. 1-8
Author(s):  
Hasan Haci Yeter ◽  
Berfu Korucu ◽  
Elif Burcu Bali ◽  
Ulver Derici
Keyword(s):  
Oxidative Stress ◽  
Vitamin D ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Stress Index ◽  
Total Oxidant Status ◽  
Vitamin D Analog ◽  
Total Antioxidant ◽  
Oxidant Status ◽  
And Oxidative Stress

Abstract. Background: The pathophysiological basis of chronic kidney disease and its complications, including cardiovascular disease, are associated with chronic inflammation and oxidative stress. We investigated the effects of active vitamin D (calcitriol) and synthetic vitamin D analog (paricalcitol) on oxidative stress in hemodialysis patients. Methods: This cross-sectional study was composed of 83 patients with a minimum hemodialysis vintage of one year. Patients with a history of any infection, malignancy, and chronic inflammatory disease were excluded. Oxidative markers (total oxidant and antioxidant status) and inflammation markers (C-reactive protein and interleukin-6) were analyzed. Results: A total of 47% (39/83) patients were using active or analog vitamin D. Total antioxidant status was significantly higher in patients with using active or analog vitamin D than those who did not use (p = 0.006). Whereas, total oxidant status and oxidative stress index were significantly higher in patients with not using vitamin D when compared with the patients who were using vitamin D preparation (p = 0.005 and p = 0.004, respectively). On the other hand, total antioxidant status, total oxidant status, and oxidative stress index were similar between patients who used active vitamin D or vitamin D analog (p = 0.6; p = 0.4 and p = 0.7, respectively). Conclusion: The use of active or selective vitamin D analog in these patients decreases total oxidant status and increases total antioxidant status. Also, paricalcitol is as effective as calcitriol in decreasing total oxidant status and increasing total antioxidant status in patients with chronic kidney disease.

Antioxidant status of blood in patients with vibration disease

2019 ◽  
pp. 978-982 ◽  
Author(s):  
N. N. Malyutina ◽  
A. F. Bolotova ◽  
R. B. Eremeev ◽  
A. Zh. Gilmanov ◽  
D. Yu. Sosnin
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Main Group ◽  
Biologically Active ◽  
Antioxidant Systems ◽  
Control Group ◽  
Vibration Disease ◽  
Total Antioxidant

Introduction. The overwhelming number of publications contains only data on the content of individual antioxidants, but not on the overall antioxidant activity of the blood in patients with vibration disease.The aim of the study was to determine the total antioxidant activity of blood serum in patients with vibration disease.Materials and methods. Th e main group consisted of 30 people diagnosed with “Vibration disease” of 1 degree (n=21) and 2 degrees (n=9). Th e control group consisted of 30 clinically healthy men, comparable in age with the main group (p=0.66). Th e total activity of antioxidant systems of blood plasma was evaluated photometrically using the test system “Total antioxidant status-Novo” (“Vector-best”, Russia).Results. The indicator of the total antioxidant status (TAS) was 1,038±0.232 mmol/l in the examined main group, against 1,456±0.225 mmol/l in the examined control group (p<0.000001). Th e coefficient of variation (CV) in patients with vibration disease was 22.35%, 1.45 times higher than in the control group (15.45%). In the main group there was a positive correlation between age and TAS (R=0.525), in the control group there was no such relationship (R=0.095). Th e degree of decrease depended on the severity of vibration disease.Conclusions. 1. The development of vibration disease is accompanied by a decrease in the antioxidant status of blood serum. 2. Th e degree of decrease in the antioxidant status of blood serum correlates with the severity of vibration disease. 3. Reduction of TAS can serve as a pathogenetic justification of the need to include drugs and/or biologically active additives with antioxidant activity in therapy

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