Antioxidant status in streptozotocin induced diabetic rats treated with vanadium complex

As lipid peroxidation and oxidative stress play a key role in the pathogenesis of diabetes, the antioxidant status of streptozotocin induced diabetic rats, treated with vanadium complex was explored in the present study. Diabetes was induced by single intraperitoneal injection of streptozotocin (STZ) at the dose rate of 45 mg per kg body weight. Diabetes was confirmed after 72 hours of STZ injection by estimating blood glucose level and those rats showing more than 250 mg/ dL were considered as diabetic. Vanadium complex at the dose rates of 5 and 10 mg / kg body weight was administered orally to normal control and STZ induced diabetic rats. Glimepiride was used as the positive control and was given orally at the dose rate of 800 μg / Kg body weight. The study on the hepatic, renal and pancreatic tissues showed that vanadium complex at both the predetermined dosages significantly increased the antioxidant activities of superoxide dismutase and glutathione peroxidase along with a significant increase in the level of glutathione and a significant decrease in the level of lipid peroxidation. The study also revealed that there is a significant reduction in the activity of catalase after treatment with vanadium complex at both the dosage levels.

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Modulatory effects of garlic, ginger, turmeric and their mixture on hyperglycaemia, dyslipidaemia and oxidative stress in streptozotocin–nicotinamide diabetic rats

British Journal Of Nutrition ◽

10.1017/s0007114510004927 ◽

2010 ◽

Vol 105 (8) ◽

pp. 1210-1217 ◽

Cited By ~ 51

Author(s):

Hafez R. Madkor ◽

Sherif W. Mansour ◽

Gamal Ramadan

Keyword(s):

Oxidative Stress ◽

Metabolic Syndrome ◽

Body Weight ◽

Antioxidant Activities ◽

Cardiovascular Complications ◽

Diabetic Rats ◽

The Metabolic Syndrome ◽

Atherogenic Indices ◽

Antioxidant Defence System ◽

And Oxidative Stress

Spices which show hypoglycaemic, hypolipidaemic and antioxidant activities may have a role in the treatment of diabetes and its complications. The present study aimed to compare the modulatory effects of garlic, ginger, turmeric and their mixture on the metabolic syndrome and oxidative stress in streptozotocin (STZ)–nicotinamide diabetic rats. Diabetes was induced in overnight fasted rats by a single intraperitoneal injection of STZ (65 mg/kg body weight) and nicotinamide (110 mg/kg body weight, 15 min before STZ injection). Diabetic rats orally received either distilled water (as vehicle) or 200 mg/kg body weight of garlic bulb, ginger rhizome or turmeric rhizome powder suspension separately or mixed together (GGT mixture) for twenty-eight consecutive days. The results showed that these spices and their mixture significantly alleviated (80–97 %,P < 0·05–0·001) signs of the metabolic syndrome (hyperglycaemia and dyslipidaemia), the elevation in atherogenic indices and cellular toxicity in STZ–nicotinamide diabetic rats by increasing the production of insulin (26–37 %), enhancing the antioxidant defence system (31–52 %, especially GSH) and decreasing lipid peroxidation (60–97 %). The greatest modulation was seen in diabetic rats that received garlic and the GGT mixture (10–23 % more than that in the ginger and turmeric groups). In conclusion, garlic or the mix including garlic appears to have an impact on each of the measures more effectively than ginger and turmeric and may have a role in alleviating the risks of the metabolic syndrome and cardiovascular complications.

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Protective role of Sterculia tragacantha aqueous extract on pancreatic gene expression and oxidative stress parameters in streptozotocin-induced diabetic rats

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2021-0020 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Basiru Olaitan Ajiboye ◽

Babatunji Emmanuel Oyinloye ◽

Jennifer Chidera Awurum ◽

Sunday Amos Onikanni ◽

Adedotun Adefolalu ◽

...

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Protective Role ◽

Diabetic Rats ◽

Gene Expressions ◽

Ki 67 ◽

Oxidative Stress Parameters ◽

And Oxidative Stress ◽

Stress Parameters

Abstract Objectives The current study evaluates the protective role of aqueous extract of Sterculia tragacantha leaf (AESTL) on pancreatic gene expressions (insulin, PCNA, PDX-1, KI-67 and GLP-1R) and oxidative stress parameters in streptozotocin-induced diabetic rats. Methods Diabetes mellitus was induced into the experimental Wistar animals via intraperitoneal (IP) injection of streptozotocin (35 mg/kg body weight) and 5% glucose water was given to the rats for 24 h after induction. The animals were categorized into five groups of 10 rats each as follows normal control, diabetic control, diabetic rats administered AESTL (150 and 300 mg/kg body weight) and diabetic rats administered metformin (200 mg/kg) orally for two weeks. Thereafter, the animals were euthanized, blood sample collected, pancreas harvested and some pancreatic gene expressions (such as insulin, PCNA, PDX-1, KI-67, and GLP-1R)s as well as oxidative stress parameters were analyzed. Results The results revealed that AESTL significantly (p<0.05) reduced fasting blood glucose level, food and water intake, and lipid peroxidation in diabetic rats. Diabetic rats administered different doses of AESTL showed a substantial upsurge in body weight, antioxidant enzyme activities, and pancreatic gene expressions (insulin, PCNA, PDX-1, KI-67, and GLP-1R). Conclusions It can therefore be concluded that AESTL has the ability to protect the pancreas during diabetes mellitus conditions.

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Inhibition ofα-Glucosidase by Thiosulfinate as a Target for Glucose Modulation in Diabetic Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/7687915 ◽

2016 ◽

Vol 2016 ◽

pp. 1-5 ◽

Cited By ~ 6

Author(s):

Abdulrahman L. Al-Malki

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Hydrolytic Enzyme ◽

Positive Control ◽

Diabetic Rats ◽

Postprandial Hyperglycemia ◽

Postprandial Blood Glucose ◽

Control Group ◽

Albino Rats ◽

Predisposing Factor

Postprandial hyperglycemia is a predisposing factor for vascular dysfunction and organ damage.α-glucosidase is a hydrolytic enzyme that increases the glucose absorption rate and subsequently elevates blood glucose levels. Garlic (Allium sativumL.) is a rich source of several phytonutrients, including thiosulfinate (THIO). The aim of this study was to evaluate the ability of THIO, a potent inhibitor of intestinalα-glucosidase, to reduce postprandial blood glucose. Male albino rats were randomly assigned to five different groups (n=10/group). Group 1 served as the control group. Groups 2–5 were injected intraperitoneally with a single dose of streptozotocin (STZ) to induce diabetes. Group 2 comprised untreated diabetic rats. Groups 3 and 4 contained diabetic rats that were given THIO orally (20 mg/kg body weight/day and 40 mg/kg body weight/day, resp.). Group 5 was the positive control having diabetic rats treated orally with acarbose (10 mg/kg body weight/day; positive control). Diabetic rats treated with THIO displayed a significant blood glucose reduction (p<0.001and < 0.01 by analysis of variance, resp.) and a significant elevation in insulin compared with that of untreated rats. THIO is an effective noncompetitive intestinalα-glucosidase inhibitor that promotes hypoglycemic action (p<0.001) in STZ-injected rats. THIO is a promising agent for the management of postprandial hyperglycemia.

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Anti-hyperglycaemic effects of dioxidovanadium complex cis-[VO2(obz)py] avert kidney dysfunction in streptozotocin-induced diabetic male Sprague–Dawley rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2020-0278 ◽

2021 ◽

pp. 1-9

Author(s):

Bonisiwe Mbatha ◽

Andile Khathi ◽

Ntethelelo Sibiya ◽

Irvin Booysen ◽

Patrick Mangundu ◽

...

Keyword(s):

Blood Glucose ◽

Kidney Injury ◽

Diabetic Control ◽

Positive Control ◽

Diabetic Rats ◽

Vanadium Complex ◽

Sprague Dawley ◽

Kidney Dysfunction ◽

Urinary Glucose ◽

Blood Glucose Concentrations

Despite the success of antidiabetic drugs in alleviation of hyperglycaemia, diabetic complications, including renal dysfunction, continue to be a burden. This raises the need to seek alternative therapies that will alleviate these complications. Accordingly, the aim of this study was to investigate the effects of dioxidovanadium(V) complex cis-[VO2(obz)py] on renal function in diabetic rats. Streptozotocin-induced diabetic rats were treated with cis-[VO2(obz)py] (40 mg·kg–1) twice every third day for five weeks. Diabetic untreated and insulin-treated rats served as the diabetic control and positive control, respectively. Blood glucose concentrations, water intake, urinary output, and mean arterial pressure (MAP) were monitored weekly for five weeks. Rats were then euthanized, and blood and kidney tissues were collected for biochemical analysis. Significant decreases in blood glucose concentrations, MAP, glomerular filtration rate (GFR), and SGLT2 expression, as well as plasma angiotensin and aldosterone concentrations, were observed in the treated groups compared with the diabetic control. The complex also increased urinary glucose concentrations, antioxidant enzymes GPx and SOD concentrations, and decreased MDA concentrations and kidney injury molecule (KIM-1) concentrations. These findings suggest that the anti-hyperglycaemic effects of this vanadium complex may ameliorate kidney dysfunction in diabetes.

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Corrigendum to “Protective role of Moringa oleifera leaf extract on cardiac antioxidant status and lipid peroxidation in streptozotocin induced diabetic rats” [Heliyon 5, (12), (December 2019), e02935]

Heliyon ◽

10.1016/j.heliyon.2019.e03146 ◽

2020 ◽

Vol 6 (1) ◽

pp. e03146 ◽

Cited By ~ 2

Author(s):

B.Y. Aju ◽

R. Rajalakshmi ◽

S. Mini

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Status ◽

Leaf Extract ◽

Moringa Oleifera ◽

Protective Role ◽

Diabetic Rats

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Antioxidant status and lipid peroxidation after short-term maximal exercise in trained and untrained humans

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1997.272.4.r1258 ◽

1997 ◽

Vol 272 (4) ◽

pp. R1258-R1263 ◽

Cited By ~ 5

Author(s):

N. Ortenblad ◽

K. Madsen ◽

M. S. Djurhuus

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Enzyme ◽

Antioxidant Status ◽

Antioxidant Activities ◽

Antioxidant Enzyme Activities ◽

Muscle Enzyme ◽

Jump Exercise ◽

Jump Test ◽

Coupled Enzymes ◽

Resting Muscle

The purpose of this study was to measure resting muscle and blood antioxidant status in untrained (n = 8) and jump-trained (n = 8) humans and to evaluate free radical-mediated muscle damage after a strenuous jump test consisting of six bouts of 30-s continuous jumping separated by 2 min of rest. Resting muscle antioxidant activities [superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GR), and manganese SOD] were significantly higher in jump-trained compared with untrained subjects. Blood antioxidant enzyme activities and muscle catalase, however, were not different between the two groups. Creatine kinase activities increased significantly (P < 0.0001) after the jump test in untrained individuals, but remained unchanged in the jump trained. Plasma and muscle malonaldehyde (MDA) after the jump test were not significantly different from rest. These data suggest that jump training is associated with elevated activities of SOD and the coupled enzymes GPX and GR in muscle tissue, but other antioxidants remain unchanged. High-intensity jump exercise induces muscle enzyme leakage in untrained humans, but muscle lipid peroxidation, measured as changes in MDA, was not different in the two groups despite the varied muscle antioxidant enzyme levels.

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