Efficacy of Bacillus Subtilis (GalliPro) supplementation in Clostridium perfringens challenged necrotic enteritis of broiler chicken

Author(s):  
Deepak Koli ◽  
Mukund Kadam ◽  
Mayura Gole ◽  
Swati Hajare ◽  
Amit Yeskal ◽  
...  

The experiment was designed for 42 days to assess dietary supplementation of Bacillus Subtilis (GalliPro-DSM 17299) on growth performance and intestinal health against C. perfringens induced necrotic enteritis in broiler birds. Birds (n=400) were divided in to four groups [healthy (control), infected, healthy supplemented with B. subtilis @ 500 g/T of feed (1.2 x 106 cfu/g); infected supplemented with B. Subtilis@ 500 g/T ] with five replicates of 20 birds in each group. Necrotic enteritis was induced in broiler birds via inoculation of 50,000 oocysts of mixed strains of Eimeria species on 14 days of age followed by C. perfringens (107 cfu/mL) on 17, 18 and 19 days of age. Study revealed that necrotic enteritis challenge suppressed the body weight gain significantly, whereas infected birds supplemented with Bacillus subtilis showed significantly higher body weight gain. The feed intake and feed conversion ratio was not significantly affected in any of the groups. Significant reduction was noticed in bacterial count of C. perfringens infected birds supplemented with B. subtilis. Intestinal damage was observed in the infected control birds while it was recovered in birds supplemented with B. subtilis. The significant beneficial effect of B. Subtilis was recorded in the production performance of broiler chicken even in the absence of necrotic enteritis challenge. Thus it was concluded that supplementation of B. Subtilis helps to improve body weight gain and gut health to control necrotic enteritis.

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Amit K. Singh ◽  
Birendra Mishra ◽  
Michael R. Bedford ◽  
Rajesh Jha

Abstract Background This study evaluated the effects of supplemental xylanase and xylooligosaccharides (XOS) in a corn-soybean meal (SBM)-based diet on growth performance and intestinal health of broilers. A total of 288 day-old chicks (Cobb 500) were allocated to 36 floor pens (8 birds/pen) equally in 9 dietary treatments in a 3 × 3 factorial arrangement. The treatments were combinations of 3 levels of xylanase (0, 0.005% and 0.01% Econase XT) and 3 levels of prebiotics (0, 0.005% and 0.01% XOS) added to basal mash diets formulated in three phases (starter, d 0–14; grower, d 15–28; finisher, d 29–42). The feed intake and body weights were recorded weekly. On d 42, ileal sections were collected for histomorphometric and gene expression analysis, and cecal content was collected for determining short-chain fatty acids (SCFA) and microbiota. Results Xylanase linearly (P < 0.01) increased the average daily gain (ADG) in both the finisher and total period and the final body weight gain (FBWG, 2940 & 2932 vs. 2760 g) of broilers. XOS did not significantly increase either ADG or FBWG (P > 0.05). Supplemental xylanase and XOS did not affect average daily feed intake and feed conversion ratio (P > 0.05). Xylanase and XOS did not change villus height (VH) or crypt depth (CD) ratio (P > 0.05). However, xylanase exhibited a trend (P = 0.097) on VH:CD ratio. The inclusion of 0.01% XOS without xylanase increased the level of IL-10 (a marker of anti-inflammatory cytokine) and IL-4 (a T-cell differentiation cytokine) genes compared with control (P < 0.05). The acetate production was increased by xylanase (P < 0.01) and XOS (P < 0.05) without an additive effect. Xylanase increased total SCFA (P < 0.01) while XOS had a tendency to increase (P = 0.052). Alpha and beta diversity of microbiota among treatments were not different (P > 0.05). However, the mean proportion of family Ruminococcaceae was increased by the supplemental 0.01% xylanase (P < 0.01). Conclusion It can be concluded that XOS can enhance cecal fermentation, while xylanase can increase the body weight gain along with the fermentation metabolites in the ceca of broilers fed the corn-SBM-based diet but the effects may not always translate into an improved mucosal absorptive capacity and a better feed efficiency.


Author(s):  
B. Prakash ◽  
S. V. Rama Rao ◽  
M.V.L.N. Raju ◽  
C. Sreenivasa Reddy

The study was conducted to determine the efficacy of organic Se produced through Saccharomyces cerevisiae from sodium selenite in the diet of Vanaraja and commercial broiler chicken. The trial was conducted in day-old Vanaraja chicks (n=160), which were randomly distributed into 4 dietary groups having 5 chicks in each of 8 replicates. The basal diet (BD) was formulated and supplemented with calculated quantity of organic Se to formulate the different experimental diets and fed up to 5 weeks. The second experiment in day-old commercial broiler chicks (n=350), which were randomly distributed into 7 dietary groups having 5 chicks in each of 10 replicates. The experimental birds were fed BD (diet I) with 0.2 mg/kg organic Se (diet II), 0.4 mg/kg organic Se (diet III) and 0.6 mg/kg organic Se (diet IV), and BD with 0.2 mg/kg inorganic Se (diet V), 0.4 mg/kg inorganic Se (diet VI) and 0.6 mg/kg inorganic Se (diet VII) up to 6 weeks. The body weight gain during 5th week was higher (P Lass Than 0.05) in the birds fed with diet supplemented 0.3 mg/kg of the organic Se compared to the other groups. However, feed intake and feed conversion ratio (FCR) among different groups did not vary (P>0.05) throughout the experiment. The activity of glutathione peroxidase was higher (P Lass Than 0.01) among the groups fed the diets containing organic Se (0.2, 0.3 and 0.4 mg/kg) compared to the control diet in experiment I. In experiment II, body weight gain and FCR at the end of the experiment were improved significantly (P Lass Than 0.01) in the group fed 0.2 mg/kg organic Se compared to control. Alanine transaminase, Aspartate aminotransferase, triglycerides and cholesterol levels were lower in groups fed diets supplemented organic Se compared to control or inorganic Se fed groups. Whereas, glutathione reductase activity was significantly higher among the groups supplemented organic Se compared to other dietary groups. Based on the results it can be concluded that supplementing organic Se up to 0.3 mg/kg enhanced the body weight gain and anti-oxidant enzyme activity in Vanaraja and commercial broiler chicken.


2012 ◽  
Vol 34 (1) ◽  
pp. 21
Author(s):  
Hardi Julendra ◽  
Zuprizal (Zuprizal) ◽  
Supadmo (Supadmo)

<p>The objective of this research was to study the use of earthworm meal (Lumbricus rubellus) (TCT) as feed additive on broiler performance, blood profile and protein digestibility. The experiment used 100 day old chicken of cobb strain (CP 707), for 35 days feeding trial. The birds were randomly divided into four dietary treatments (R0: without TCT addition, R1: 0.5% TCT addition, R2: 1% TCT addition, R3: 1.5% TCT addition) with five replications, wich consisted five chickens each. The experimental design used Completely Randomized Design (CRD) of one way ANOVA then continued with Duncan test. Performance test was conducted by recording body weight gain, feed consumption and feed efficiency. Blood profile test was obtained by counting the number of erythrocyte, leukocyte and lymphocytes, whereas protein digestibility test used the collection method of excreta and ileum content. The result showed that feed consumption of R1 (2,065 g), R2 (2,025.4 g), R3 (2,075.3 g) were significantly different (P&lt;0.05) from<br />R0 (2,309.8 g), while the body weight gain were not different among treatments and FCR of R1 (1.78), R2 (1.78), R3 (1.78) were significantly different (P&lt;0.05) from R0 (2.04). The result of blood profile test showed that the number of<br />erythrocyte among treatments were not significantly different, the number of leukocyte of R1 (18,575 μl), R2 (18,200 μl), R3 (20,175 μl) were found significantly higher (P&lt;0.05) than R0 (16,675 μl) and lymphocytes of R1 (22.7%), R2 (21.8%), R3 (23.6%) were significantly higher (P&lt;0.05) than R0 (15.2%). Protein digestibility of R3 (91.69%) was significantly different (P&lt;0.05) from R0 (81.75%), R1 (90.48%) and R2 (85.99%), while R1 (90.48%) was significantly different (P&lt;0.05) from R0 (81.75%), but R2 (85.99%) was not significantly different from R0 (81.75%). It could be inferred that earthworm meal addition as feed additive could improved production performance of broiler, the poultry health through blood profile, and enhanced the protein digestibility.</p><p>(Key words: Feed additive, Earthworm meal, Broiler)<br /><br /></p>


2018 ◽  
Vol 16 (2) ◽  
pp. 201-210
Author(s):  
Muryanto Muryanto ◽  
Pita Sudrajad ◽  
Amrih Prasetyo

The aim of the study was to determine the development of ramie plants (Boehmeria nivea L. Gaud) and the effect of using ramie leaves on feed on the body weight gain of Wonosobo Sheep (Dombos). Research on the development of ramie plants using survey methods in the area of ramie plant development in Wonosobo Regency. While the research on the use of ramie leaves for fattening was carried out in Butuh Village, Kalikajar District, Wonosobo Regency in 2018. 21 male Dombos were divided into 3 feed treatments with forage proportions of 70%, 50% and 30 ramie leaves respectively. %. The results showed that currently ramie plants were being developed in Wonosobo Regency by CV. Ramindo Berkah Persada Sejahtera in Gandok Village, Kalikajar District, Wonosobo Regency, Central Java. Until now the area of the crop has reached 13 ha. Of this area will produce ramie leaves 195,000 kg / year. If one sheep needs 4 kg of ramie / tail / day leaves, then the potential capacity of sheep is 135 heads / year, if the given one is 50% then the Jurnal Litbang Provinsi Jawa Tengah, Volume 16 202 Nomor 2 – Desember 2018potential capacity is 270 heads / year and if it is reduced again to 25% of ramie leaves then the potential capacity 440 heads / year. The use of ramie leaves as a feed for Wonosobo Sheep fattening can be given as much as 30% in fresh form.


Author(s):  
P. M, Lunagariya ◽  
R. S. Gupta ◽  
S. V. Shah ◽  
Y. G. Patel

The study was planned to evaluate the effect of exogenous fibrolytic enzymes (EFE) supplementation for 56 days @ 240 mg/kg total mixed ration (TMR) on digestibility of dry matter and nutrients in dairy cows. Six dry non-pregnant cows were assigned in each treatment with and without EFE. The digestibility trial of seven days was conducted after 49 days of feeding. Dry matter and nutrients intake of cows was not influenced by EFE. The supplementation of EFE had improved digestibility of dry matter, organic matter, crude fiber, neutral detergent fiber, cellulose (p less than 0.01), as well as digestibility of nitrogen-free extract and acid detergent fiber, was also higher (pless than 0.05). The body weight gain of cows was higher on the supplementation of EFE in TMR. The study concluded that feeding exogenous fibrolytic enzymes (240 mg/kg) supplemented TMR improved digestibility of dry matter and nutrients, which was reflected as higher body weight gain in dry non-pregnant Gir and crossbred dairy cows.


2019 ◽  
Author(s):  
Champak Bhakat

In order to decide the optimum time of grazing for camels during hot summer months, 10 growing camel calveswere divided into 2 equal groups. First group was sent for grazing during 10:00 h to 16:00 h daily and second groupallowed for grazing during thermo neutral period. The climatic variables were recorded daily (April 2012 to March2013). The average daily gain and total body weight gain in calves sent for grazing during relatively cool parts ofday (group 2) was significantly higher as compared to group 1 calves sent as per routine farm schedule. Theaverage intake of fodder and water from manger was higher in group 1 calves. The average DMI from manger forgroup 1 calves was higher as compared to group 2 calves. The comparative biometrics of camel calves in differentgrazing management practices revealed that body length, heart girth, height at wither, neck length were significantly(P&lt;0.01) higher in group 2 calves as compared to group 1 calves. After 180 days of experimentation, humpcircumference vertical and hind leg length were significantly (P&lt;0.05) increased in group 2 as compared to group1. Analysis of recorded data of climatic parameters revealed that average maximum temperature was higher duringJune 2012. The values of THI also were higher in monsoon and post monsoon months hence the practice of sendingcamel calves during relatively comfortable part of hot and hot humid months was successful in getting good growth.The relative humidity was significantly higher during morning as compared to evening period for all months. TheTHI was significantly lower during morning as compared to evening hours for all months in different climate forwhole year. Economic analysis reveals that the cost of feed per kg body weight gain was quite less in group 2 ascompared to group 1. So the practice of grazing of camel calves during cool hours of day remain profitable forfarmers by looking at the body weight gain and better body conformation in climate change condition.


2020 ◽  
Vol 17 (35) ◽  
pp. 800-812
Author(s):  
Ilgiz DOLININ ◽  
George BAZEKIN ◽  
Evgeny SKOVORODIN ◽  
Almaz SHARIPOV ◽  
Ivan CHUDOV

Poultry farming holds a special place in ensuring the products that the consumers demand, it provides the population with essential food products,such as eggs and meat,that contain vital micro and macronutrients, proteins, lipids, and vitamins. Therefore, the issues of rational, economically feasible feeding of meat poultry, namely broiler chickens, are an urgent task. It is also essential to find effective methods of their application in order to correct the natural resistance and immune and biological reactivity of birds. The purpose of this research is to study the effect of the biological stimulant-Nucleostim on the growth and development of chickens, hematological, and immunological parameters of the blood of birds.This Biostimulant is a purified bovine spleen extract containing at least 1 mg / ml of low molecular weight peptides (nucleotides and nucleosides) formed as a result of autolysis, using dry whey and diatomite as fillers. Onthe application ofNucleostim, the gain in live weight of chickens was increased by 9.7%. At the end of the experiment, the livability of the chicks of the experimental group treated with Nucleostimcame up to 88%, compared with the 72% of the control group. The use of biostimulant had a stimulating effect on the liver of chickens confirmed by the research results presented in the article, as well as contributed to the development of the thymus in the setting of general dystrophy. Thus, it improved chicklivability and increased body weight gain. The biological stimulant-Nucleostim as an adaptogenic, anabolic, and immunostimulatory agent is promising for finding new drugs that improve the health and productivity of poultry.


2021 ◽  
Vol 888 (1) ◽  
pp. 012072
Author(s):  
Siti Khairunnisa ◽  
Sumiati ◽  
Cece Sumantri ◽  
Wiwin Winarsih

Abstract This study aimed to evaluate the effect of zinc supplementation in various diets on the performance and health status of IPB-D2 chickens. Subsequently, about 180 one-week-old IPB-D2 chickens were used in the study and were fed the treatment diets for 12 weeks. Also, this study employed a completely randomised factorial design with six treatments and three replications of ten birds each. These treatment diets included A1, which contained nutrients based on SNI (2013) without Zn supplementation, A2 which is a combination of A1 and 60 ppm of Zinc, B1 which contained nutrients 5% higher than SNI without Zn supplementation, B2 which is a combination of B1 and 60 ppm Zinc, C1 which contains nutrients 10% higher than SNI without Zn supplementation, and lastly, C2 which is a combination of C1 and 60 ppm of Zinc. Furthermore, the data were analysed using ANOVA, and the parameters observed were the body weight, body weight gain, feed consumption, feed conversion ratio, blood profile and the villi surface area of the jejunum. Consequently, the results showed that C2 significantly (P<0.05) increased the body weight and body weight gain when fed to the finisher and grower chickens, respectively. More so, when the finisher chickens were fed the 10% SNI diet, there was an increased body weight gain and also a significantly (P<0.05) decreased feed conversion ratio. Furthermore, the addition of 60 ppm zinc increased haemoglobin significantly (P<0.05), whereas C2 decreased leukocytes significantly (P<0.05) in chicken blood. However, the treatments had no effect on feed consumption in the villi surface area of the jejunum. Therefore, the study concluded that supplementing the IPB-D2 chickens with 60 ppm zinc in a 10% SNI diet resulted in the best performance and health status.


2001 ◽  
Vol 2 (2) ◽  
pp. 26-31
Author(s):  
Al Qudri

ABSTRACT. Tempe of soybean is fermented soybean with Rhizopus ologosporus fungus. From a result of researchers, it is the fact that tempe can increase valuable of nutrion and digest capacity and also produce antibiotic . Utilization of abtibiotic in chiken food as stimulus growth in not recommended because it can cause resistance a certain germ and it is presume there are antibiotic residu in egg and chiken meat. It is hope that the use of fermented soybean oil meal can increase nutrient value and digest capacity as well as produce antibiotic, so that it can get a better growth and over come a problem of using antibiotic in chiken food. The study was conducted to evaluate the effect of fermented soybean oil meal utilization in the ration on body weight gain, feed consumption, feed conversion and mortality. The study use 80 unsex day old chick broiler CP-707 and conducted at Poulty Laboratory Product Agriculture Faculty, Syiah Kuala University. Chiken were putting in 27 houses unit, each unit contain of four chiken. The design of experiment was Completely Randomized Design, four treatments and five replication. The treatments were ration contained 0%, 5%, 10% and 15% fermented soybean oil meal as replace the use of soybean oil meal. The protein and energy content of all dietary treatments were equally composed from six weeks investigation, were body weight gain, feed consumption, feed conversion and mortality. The result of the study indicated that up to 10% graded of fermented soybean oil meal in ration were significant difference influenced increase the body weight gain and decrease the feed conversion. The fermented soybeanoil meal) utilization more than 10% were significant difference decrease the body weight gain and increase the feed conversion. If the inclusion was increase the feed consumption would tendency decline. In this study there were no mortality in all treatment. In can be advised from this study that fermented soybean oil meal could be used in broiler ration or in all poulty ration.


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