Herbal Essential Oils Along with Its Amelioration with Silver Nanoparticles for Curing the Canine Demodicosis

2021 ◽  
Author(s):  
Moneesh Thakur ◽  
Hridayesh Prasad ◽  
A.K. Samanta ◽  
Anu Kalia
Keyword(s):  
Silver Nanoparticles ◽  
Essential Oils ◽  
Research Work ◽  
Accession Number ◽  
Skin Scraping ◽  
Demodex Canis ◽  
Pcr Product ◽  
Pcr Products ◽  
Nano Medicine ◽  
The Ideal

Background: Demodicosis, also named as demodectic mange, red mange or follicular mange (Shrestha et al., 2015). In dogs, Demodex canis is acquired from the dam during the first few hours of life, probably during suckling (Greve and Gaafar, 1966). Demodicosis can be defined on the basis of two forms localized and generalized (Shipstone, 2000) with juvenile or adult onset. The ideal confirmation of diagnosis of demodicosis were established by the laboratory analysis of the cutaneous skin scrapings. Various drugs have been used for treating canine demodicosis. Till date no research work has been done on herbal nano medicine against demodicosis in dogs especially in Mizoram. Keeping these points in view the present study was made to formulate the herbal nanomedicine against demodicosis in dogs.Methods: Present investigation was conducted for curing canine demodicosis with the help of herbal essential oils along with its ameloriation with silver Nanoparticles. A total of 1200 dogs were screened for canine demodicosis and 35 cases were confirmed for canine demodicosis by skin scraping and amp; PCR examination.Result: The typical characteristics of Demodex spp. were confirmed in (20/35) 57.14% cases by skin scraping examination while PCR examination demonstrated (35/35) 100% by the amplification of an approximately 483 bp. Sequencing of PCR products were analyzed by BLAST and amp; the results indicated 99.7% identical to available sequences of D. canis MG372354 (1:99.7) and 98.8 identical with D. canis KU253790 (33:98.8) and amp; MG372359 (1:96.8). The sequence of the PCR product of positive samples was submitted to NCBI GenBank for accession number and MK177513 accession number was obtained for GenBank. From the present study it seems that Herbo-Nano medicine can be an effective alternative of Amitraz in case of demodicosis.

scholarly journals Datura leaf curl betastellite associated for the first time with Datura inoxia leaf curl syndrome in India.

2014 ◽  
Vol 1 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Dr Rajneesh Prajapat ◽  
Avinash Marwal ◽  
R K Gaur
Keyword(s):  
Present Report ◽  
Plant Viruses ◽  
Accession Number ◽  
Recombinant Fragment ◽  
Circular Dna ◽  
Pcr Product ◽  
Pcr Products ◽  
First Time ◽  
Datura Inoxia ◽  
Leaf Curl

Begomovirus is one of the largest group of Bemisia tabaci transmitted plant viruses (family Geminiviridae) containing single-stranded circular DNA that encapsidated in geminate particles and prevalent in the tropical and subtropical regions of the world. In the present report we identified a begomovirus infecting Datura inoxia, a common toxic weed found in India annually. PCR product of complete betasatellite having an expected size 1.04 kb was obtained from infected plants samples. The PCR products were suitably cloned into pGEM-T vector and sequenced, having Accession number as JQ693149 (Datura leaf curl betasatellite). The betasatellite had showed highest nucleotide sequence identities of 93 % with Croton yellow vein mosaic betasatellite (HM143908). Rcombination analysis with RDP v.3.44 clearly indicates the portion of recombinant fragment of betasatellite infecting Datura inoxia is contributed from the two viruses prevailing at different geographical region, undoubtedly pointing towards the betasatellite evolution.

COMPARATIVE LITERATURE: EAST AND WEST STUDIES

2021 ◽  
Vol 02 (09) ◽  
pp. 8-14
Author(s):  
Aziza Komilovna Akhmedova ◽  
Keyword(s):  
Comparative Literature ◽  
Professional Ethics ◽  
Research Work ◽  
Literary Studies ◽  
The Novel ◽  
World Science ◽  
History Of ◽  
The Aesthetic ◽  
Aesthetic Ideal ◽  
The Ideal

The article analyzes the results of the research on the representation of the aesthetic ideal through the image of the ideal hero in two national literatures. For research purposes, attention was paid to highlighting the category of the ideal hero as an expression of the author's aesthetic views. In Sinclair Lewis’s “Arrowsmith” and Pirimkul Kodirov's “The Three Roots”, the protagonists artistically reflect the authors' views on truth, virtue, and beauty. In these novels, professional ethics is described as a high noble value. The scientific novelty of the research work includes the following: in the evolution of western and eastern poetic thought, in the context of the novel genre, the skill, common and distinctive aspects of the creation of an ideal hero were revealed by synthesis of effective methods in world science with literary criteria in the history of eastern and western literary studies, in the example of Sinclair Lewis and Pirimkul Kodirov.

BIOSYNTHESIZED SILVER NANOPARTICLES USING CATHARANTHUS ROSEUS AND THEIR ANTIBACTERIAL EFFICACY IN SYNERGY WITH ANTIBIOTICS; A FUTURE ADVANCEMENT IN NANOMEDICINE

2021 ◽  
pp. 116-124
Author(s):  
MONIKA GUPTA
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Catharanthus Roseus ◽  
Leaf Extract ◽  
Research Work ◽  
Resistant Bacteria ◽  
Surface Plasmon Resonance Peak ◽  
Plasmon Resonance Peak ◽  
X Ray ◽  
Uniform Dispersion

Objective: This research work develops an approach to synthesize silver nanoparticles (AgNPs) by reduction of leaf extract of Catharanthus roseus plant. This study produces synthesized nanoparticles that have process-controlled attributes which make their antibiotic action highly efficient. These attributes include smaller size, proper morphology, uniform dispersion, metal ion content, and formation of functional groups. By optimizing the reduction process parameters, AgNPs gain the desired properties.  Methods: The biosynthesis of AgNPs process was performed using reaction of 10% (w/v) C. roseus leaf extract with AgNO3. The optimum conditions and concentration used for synthesis of nanoparticles were: 1 mM AgNO3, pH 5, and temperature 80°C with an incubation time of 72 h. All the above parameters were analyzed by ultraviolet-visible spectrophotometer with the surface plasmon resonance peak obtained at 440 nm. Results: Various characterization techniques were performed, namely, scanning electron microscopy, energy-dispersive X-ray, transmission electron microscopy, photoluminescence study, X-ray diffraction spectroscopy, Fourier transform infrared, dynamic light scattering, and atomic force microscopy. The results obtained from characterization confirmed the spherical morphology of the nanoparticles with size between 50 and 87 nm. In the current investigation, the antimicrobial activity of biosynthesized AgNPs was also determined using minimum inhibitory concentration and zone of inhibition methods against six different bacteria at different doses of AgNPs (100, 150, and 200 μg/ml) alone and also in combination with antibiotic-streptomycin. Conclusion: The results revealed that high concentration of AgNPs inhibits the bacterial growth. Furthermore, AgNPs revealed much stronger antibacterial action in synergy with streptomycin against antibiotic-resistant bacteria.

scholarly journals The Literary «Ekphrasis» of Castel Nuovo: A Contribution to Value the Cultural Identity of the Ideal City Government, Promoted by Aristocratic Courts and Civil Administrations in the XIV Century

2014 ◽  
Vol 11 ◽  
pp. 483-488
Author(s):  
Sara Cipolla
Keyword(s):  
Cultural Identity ◽  
Research Work ◽  
Italian Literature ◽  
City Government ◽  
The Other ◽  
The Political ◽  
Ripe Fruit ◽  
Surface Projection ◽  
Medieval Italian Literature ◽  
The Ideal

The research work concerns the development in the Italian literature of the French theme of Neuf Preux, and Particularly Took into account a crown of sonnets of nine famous men linked to an alleged cycle of paintings attributed to Giotto's in the palace of Castel Nuovo in Naples. The survey highlighted how in medieval Italian literature, beyond the more or less explicit recovery of the French literary tradition, occupies a prominent place the function that these poems take in the view of the literature of the time. The survey actually shows the two faces of the series of famous heroes, which on one hand is the mouthpiece of the political ideals and civil inspired by the courteous and Roman antiquities, on the other hand appears to be ripe fruit of a didactic poem in which the adherence to the motto of "ut pictura poesis" become as a kind of surface projection of images.

scholarly journals A PCR-Based Method of Detection and Differentiation of K88+ Adhesive Escherichia Coli

1996 ◽  
Vol 8 (4) ◽  
pp. 460-463 ◽  
Author(s):  
Mark A. Franklin ◽  
David H. Francis ◽  
Diane Baker ◽  
Alan G. Mathew
Keyword(s):  
Escherichia Coli ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigenic Variant ◽  
Variable Regions ◽  
E Coli ◽  
Clinical Assay ◽  
Pcr Product ◽  
Structural Subunit ◽  
Pcr Products ◽  
Polymerase Chain

The objective of this study was to develop a polymerase chain reaction (PCR)-based method to detect and differentiate among Escherichia coli strains containing genes for the expression of 3 antigenic variants of the fimbrial adhesin K88 (K88ab, K88ac, and K88ad). Five primers were designed that allowed detection of K88+ E. coli, regardless of antigenic variant, and the separate detection of the ab, ac, and ad variants. Primers AM005 and AM006 are 21 base pair (bp) oligomers that correspond to a region of the K88 operon that is common to all 3 antigenic variants. Primers MF007, MF008, and MF009 are 24-bp oligomers that matched variable regions specific to ab, ac, and ad, respectively. Using primers AM005 and AM006, a PCR product was obtained that corresponds to a 764-bp region within the large structural subunit of the K88 operon common to all 3 antigenic variants. Primer AM005 used with MF007, MF008, or MF009 produced PCR products approximately 500-bp in length from within the large structural subunit of the K88 operon of the 3 respective antigenic variants. Fragments were identified by rates of migration on a 1% agarose gel relative to each other as well as to BstEII-digested lambda fragments. This PCR-based method was comparable to the enzyme-linked immunosorbent assay and western blot test in the ability to differentiate between the antigenic variants. K88+ E. coli were differentiated from among laboratory strains and detected in ileal samples taken from cannulated pigs challenged with a known K88+ variant. K88+ E. coli were also detected from fecal swabs taken from newly weaned pigs, thus confirming that this PCR-based test could provide a convenient clinical assay for the detection of K88+ E. coli. Detection and differentiation of K88+ E. coli using general and specific primers was successful. PCR methods of detection should permit identification of K88+ antigenic variants regardless of the level of expression of the antigen.

scholarly journals First Report of a Group 16SrII Phytoplasma Associated with Witches'-Broom of Eggplant in Oman

Plant Disease ◽  
2011 ◽  
Vol 95 (3) ◽  
pp. 360-360 ◽  
Author(s):  
A. M. Al-Subhi ◽  
N. A. Al-Saady ◽  
A. J. Khan ◽  
M. L. Deadman
Keyword(s):  
Nested Pcr ◽  
Solanum Melongena ◽  
Positive Control ◽  
Rrna Gene ◽  
Direct Pcr ◽  
First Report ◽  
Pcr Product ◽  
Pcr Products ◽  
Broom Disease ◽  
Phytoplasma Infection

Eggplant (Solanum melongena L.) belongs to the family Solanaceae and is an important vegetable cash crop grown in most parts of Oman. In February 2010, plants showing phyllody symptoms and proliferation of shoots resembling those caused by phytoplasma infection were observed at Khasab, 500 km north of Muscat. Total genomic DNA was extracted from healthy and two symptomatic plants with a modified (CTAB) buffer method (2) and analyzed by direct and nested PCR with universal phytoplasma 16S rDNA primers P1/P7 and R16F2n/ R16R2, respectively. PCR amplifications from all infected plants yielded an expected product of 1.8 kb with P1/P7 primers and a 1.2-kb fragment with nested PCR, while no products were evident with DNA from healthy plants. Restriction fragment length polymorphism (RFLP) profiles of the 1.2-kb nested PCR products of two eggplant phyllody phytoplasma and five phytoplasma control strains belonging to different groups used as positive control were generated with the restriction endonucleases RsaI, AluI, Tru9I, T-HB8I, and HpaII. The eggplant phytoplasma DNA yielded patterns similar to alfalfa witches'-broom phytoplasma (GenBank Accession No. AF438413) belonging to subgroup 16SrII-D, which has been recorded in Oman (1). The DNA sequence of the 1.8-kb direct PCR product was deposited in GenBank (Accession No. HQ423156). Sequence homology results using BLAST revealed that the eggplant phyllody phytoplasma shared >99% sequence identity with Scaevola witches'-broom phytoplasma (Accession No. AB257291.1), eggplant phyllody phytoplasma (Accession No. FN257482.1), and alfalfa witches'-broom phytoplasma (Accession No. AY169323). The RFLP and BLAST results of 16S rRNA gene sequences confirm that eggplant phyllody phytoplasma is similar to the alfalfa phytoplasma belonging to subgroup 16SrII-D. To our knowledge, this is the first report of a phytoplasma of the 16SrII-D group causing witches'-broom disease on eggplant in Oman. References: (1) A. J. Khan et al. Phytopathology 92:1038, 2002. (2) M. A. Saghai-Maroof et al. Proc. Natl. Acad. Sci. USA, 81:8014, 1984.

scholarly journals Genotyping Single-Nucleotide Polymorphisms by the Invader Assay with Dual-Color Fluorescence Polarization Detection

2001 ◽  
Vol 47 (8) ◽  
pp. 1373-1377 ◽  
Author(s):  
Tony M Hsu ◽  
Scott M Law ◽  
Shenghui Duan ◽  
Bruce P Neri ◽  
Pui-Yan Kwok
Keyword(s):  
Fluorescence Polarization ◽  
Cost Effective ◽  
Snp Markers ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Clear Cut ◽  
Pcr Product ◽  
Invader Assay ◽  
Pcr Products ◽  
Incorporation Assay

Abstract Background: The PCR-Invader® assay is a robust, homogeneous assay that has been shown to be highly sensitive and specific in genotyping single-nucleotide polymorphism (SNP) markers. In this study, we introduce two changes to improve the assay: (a) we streamline the PCR-Invader method by assaying both alleles for each SNP in one reaction; and (b) we reduce the cost of the method by adopting fluorescence polarization (FP) as the detection method. Methods: PCR product was incubated with Invader oligonucleotide and two primary probes at 93 °C for 5 min. Signal probes corresponding to the cleaved flaps of the primary probes [labeled with fluorescein and 6-carboxytetramethylrhodamine (TAMRA) dye] and Cleavase® VIII enzyme (a flap endonuclease) were then added to the mixture. This reaction mixture was incubated at 63 °C for 5 min. FP measurements were made with a fluorescence plate reader. Results: Eighty-eight individuals were genotyped across a panel of 10 SNPs, using PCR product as template, for a total of 880 genotypes. An average “no call” rate of 3.2% was observed after first round of experiments. PCR products were remade in those samples that failed to produce any genotype in the first round, and all gave clear-cut genotypes. When the genotypes determined by the PCR-Invader assay and template-directed dye-terminator incorporation assay with FP were compared, they were in 100% concordance for all SNP markers and experiments. Conclusions: The improvements introduced in this study make PCR-Invader assay simpler and more cost-effective, and therefore more suitable for high-throughput genotyping.

Automated closed-vessel system for in vitro diagnostics based on polymerase chain reaction

1993 ◽  
Vol 39 (9) ◽  
pp. 1927-1933 ◽  
Author(s):  
J B Findlay ◽  
S M Atwood ◽  
L Bergmeyer ◽  
J Chemelli ◽  
K Christy ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pcr Amplification ◽  
Automated System ◽  
Closed Vessel ◽  
Chain Reaction ◽  
Panel Testing ◽  
Pcr Product ◽  
Pcr Products ◽  
Polymerase Chain

Abstract An automated system for polymerase chain reaction (PCR) amplification and detection combats false-positive results caused by "PCR product carryover." The system uses a single vessel for both PCR amplification and the subsequent detection of PCR products, eliminating the need to handle PCR products in an open environment and risk product carryover. The sample and PCR reagents are introduced into one compartment within the vessel, and amplification occurs as they are thermally cycled. Other compartments contain the reagents for detection of PCR products. Pressure from a roller provides for sequential delivery of the contents of the compartments to a detection area. The PCR products are biotinylated at their 5' ends during amplification through the use of biotinylated primers. After delivery to the detection area, they are specifically captured by hybridization with immobilized oligonucleotide probes. Subsequent reaction with streptavidin-horseradish peroxidase conjugate forms a complex that catalyzes dye formation from dye precursor. Wash steps minimize nonspecific background. This format is amenable to multiplexing, permitting internal controls, speciation of bacteria, typing of viruses, and panel testing. An HIV assay performed with this system demonstrated 100% sensitivity and 95% specificity for 64 patients' samples relative to a conventional PCR assay based on 32P solution hybridization. Similarly, an automated closed-vessel assay of cytomegalovirus exhibited 97.5% sensitivity and 100% specificity.

Sign in / Sign up

Export Citation Format

Share Document