scholarly journals ​Inheritance Studies and Validation of Molecular Markers Associated with Botrytis Grey Mould in Chickpea (Cicer arietinum L.)

2021 ◽  
Author(s):  
Rajneesh Bhardwaj ◽  
R.K. Panwar ◽  
A.K. Gaur ◽  
S.K. Verma ◽  
Anju Arora ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Grey Mould ◽  
Susceptible Variety ◽  
Resistant Variety ◽  
Single Marker Analysis ◽  
Chi Square ◽  
Single Marker ◽  
Selection For ◽  
High Yielding Varieties ◽  
Chi Square Analysis

Background: The Botrytis grey mould (BGM) is a devastating foliar disease of chickpea. In order to develop resistant high yielding varieties, the genetic mechanism governing the inheritance of resistance against BGM must be decoded. The molecular markers associated with BGM resistance are also need to be validated for marker assisted pyramiding of BGM resistance in chickpea. Methods: The present study was conducted during rabi seasons of 2014-18 at GBPUAT, Pantnagar. The experimental material consisted of six generations (P1, P2, F1, F2, BC1 and BC2) of a cross between a BGM resistant variety GL 10006 and susceptible variety H 208. The disease data was scored on nine-point (1-9) scale and were subjected to chi-square analysis. The inheritance was also studied by using 28 STMS markers at the Pulse Breeding Laboratory of GBPUAT, Pantnagar during 2017-18. The Single Marker Analysis was performed for validation of markers associated with BGM. Result: The results indicated that resistance for BGM in chickpea is dominant over susceptibility. A major QTL i.e. TA118 along with some minor QTLs are involved in governing resistance to BGM. The marker TA118, TS72 and TA144 can be used effectively in marker assisted selection for getting desirable recombinants in chickpea breeding.

scholarly journals Identification of Putative Molecular Markers Associated with Nut Weight and Kernel Weight and Four Growth Traits in Cashew

2020 ◽  
pp. 262-274
Author(s):  
E. Eradasappa ◽  
G. S. Mohana ◽  
J. D. Adiga ◽  
Siddanna Savadi
Keyword(s):  
Molecular Markers ◽  
Ssr Markers ◽  
Growth Traits ◽  
Tree Height ◽  
Kernel Weight ◽  
Growth And Yield ◽  
Phenotypic Variance ◽  
Single Marker Analysis ◽  
Marker Analysis ◽  
Single Marker

Aim: To identify putative molecular markers linked to some useful traits in cashew using SSR markers in the F1 population. Place and Duration of the Study: Study conducted at ICAR-Directorate of Cashew Research, Puttur, Karnataka, India during 2013-17. Methodology: Population for the study comprised of 83 F1 plants developed through crossing tall variety Ullal-3 as female parent with dwarf accession NRC-492 as male parent which was planted in the field at 6m x 6m spacing during the year 2009. It was phenotyped along with parents during the year 2017 for the 13 growth and yield related traits and genotyped using 32 polymorphic SSR markers from cashew, almond, pistachio and mango. Single marker analysis was deployed to identify the markers linked to traits by SPSS software. Results: results revealed three markers such as AL 29, IM 31 and IM 28 to be significantly associated with some traits. Marker AL 29 from Almond species was linked to traits stem girth, nut weight and kernel weight with an explained phenotypic variance of 7.9%, 5.6% and 5.4%, respectively. Marker IM 31 from mango species was associated with stem girth, kernel weight, tree height, mean tree spread, nut weight and presented phenotypic variance of 17.6%, 5.7%, 8.2%, 4.5, 4.2% in that order. While the marker IM 28 from mango species showed linked with stem girth and intermodal length with phenotypic variance of 7.6%, 7.5%, 10.4% correspondingly. Conclusion: The present study has identified three markers AL 29  (from Almond) , IM 28 and IM  31 (from Mango) linked to six traits viz., stem girth, tree height, mean tree spread, intermodal length, nut weight and kernel weight with the limited polymorphic markers using Single Marker Analysis. However, further studies are aimed to validate the linked markers prior to using them in marker assisted selection in cashew.

scholarly journals Confirmation of Alleles Inheritance in F1 Progenies Derived from a Cross of Calcutta-4 [Musa acuminata ssp. burmannicoides] and Musa acuminata ssp. microcarpa Based on SSR Markers

2020 ◽  
Vol 16 (1) ◽  
pp. 17
Author(s):  
Dea Rosalia ◽  
Puji Lestari ◽  
Andy Soegianto ◽  
Darmawan Saptadi ◽  
Agus Sutanto ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Disease Resistance ◽  
Ssr Markers ◽  
Selection Process ◽  
Musa Acuminata ◽  
Chi Square ◽  
Genetic Studies ◽  
Ssr Primers ◽  
Chi Square Analysis ◽  
Selection Of

<p>Banana breeding to produce improved varieties with disease resistance haracters and other desired traits could sustain its yield. Alleles harbored by parents could be passed on to the offsprings through hybridization, but need to be confirmed using molecular markers. This study aimed to confirm allele inheritance in F1 progenies derived from a cross of Calcutta-4 (Musa acuminata ssp.  burmannicoides) and M. acuminata ssp. microcarpa based on SSR markers. Eleven pairs of SSR primers were used to amplify DNA of 44 progenies using the PCR technique. The results showed that six SSR markers (MaSSR 1.1, MaSSR<br />5.1, MaSSR 6.1, MaSSR 7.1, MaSSR 8.1, and MaSSR 11.1) were polymorphic in both parents. Four markers (MaSSR 1.1, MaSSR 5.1, MaSSR 6.1, and MaSSR 8.1) had PIC &gt;0.7, indicating their informativeness to distinguish these progenies and other genetic studies of banana germplasms. A total of 44 F1 individuals were confirmed to harbor alleles inherited from their parents,<br />suggesting as true progenies from the cross of Calcutta-4 and M. acuminata ssp. microcarpa. This population demonstrated 100% success of hybridization performed. Chi-Square analysis revealed that segregation of all markers did not match to Mendelian ratio 1:2:1, except for MaSSR 1.1 (x2 = 5,62) and MaSSR 6.1 (x2 = 3,77) markers. The genetic traceability of banana F1 progenies demonstrating the usefulness and feasibility of SSR markers in this study provided information on selection of true progenies which may be valuable for breeders to assist selection process in future banana breeding program in Indonesia.</p>

Validation of microsatellite molecular markers linked with resistance to Bipolaris sorokiniana in wheat (Triticum aestivum L.)

2017 ◽  
Vol 155 (7) ◽  
pp. 1061-1068 ◽  
Author(s):  
B. TEMBO ◽  
J. SIBIYA ◽  
P. TONGOONA ◽  
L. TEMBO
Keyword(s):  
Molecular Markers ◽  
Bipolaris Sorokiniana ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Single Marker Analysis ◽  
Independent Population ◽  
Single Marker ◽  
Efficiency Of Selection ◽  
The Relationship ◽  
Resistant Genotypes

SUMMARYSpot blotch disease caused by Bipolaris sorokiniana (Sacc.) Shoem causes yield losses and reduces grain quality in wheat. Molecular markers reported to be linked with resistance to B. sorokiniana could accelerate the identification of resistant genotypes as they are independent of the environmental effect. However, before they can be utilized for marker assisted selection (MAS), validation in an independent population is required. The objective of the present study was therefore to validate three simple sequence repeat (SSR) molecular markers (Xwgm570, Xgwm544 and Xgwm437) linked with resistance to B. sorokiniana. The markers were validated using 66 wheat genotypes comprising 11 parental genotypes and 55 F2 progenies. Single marker analysis was performed using simple linear regression to ascertain the relationship between the marker and the trait. All the markers were confirmed to be associated with resistance. They all gave significant association with resistance to B. sorokiniana. The markers amplified DNA fragments in the resistant parental genotypes that were similar to those observed in resistant F2 progenies, but absent in the susceptible ones. Hence, these markers could be useful in increasing the efficiency of selection for resistance to B. sorokiniana in wheat breeding. Since the R2 values are low, a combination of two or three SSR markers can be employed during MAS. This was evident by the multiple linear equation which gave a combined R2 value of 18·0%, obtained from the inclusion of all three markers.

scholarly journals Factors Influencing Tooth Shade Selection for Completely Edentulous Patients

2006 ◽  
Vol 7 (5) ◽  
pp. 80-87 ◽  
Author(s):  
Temitope Ayodeji Esan ◽  
Adeyemi Oluniyi Olusile ◽  
Patricial Adetokunbo Akeredolu
Keyword(s):  
Skin Color ◽  
Scientific Information ◽  
Chi Square ◽  
Black African ◽  
Factors Influencing ◽  
Significant Difference ◽  
Selection For ◽  
Edentulous Patients ◽  
Chi Square Analysis ◽  
Shade Selection

Abstract Aim There is limited scientific information on the relationship between tooth shade, age, gender, and skin color in black Africans. This lack of knowledge may impact the ability of the prosthodontist to select artificial teeth for completely edentulous patients. This study explores the possibility of a relationship between tooth shade, gender, age, and skin color in a black African population. Methods and Materials A total of 212 individuals aged 15 to 79 years participated in this study. One investigator, calibrated for examining tooth shade, performed all examinations. A Vita-Lumin shade guide was used to examine either the maxillary right or left central incisor. One had to be sound for inclusion in the study. Tooth shades were divided into two categories according to value, and skin tones were divided into two categories (light, and dark). Chi-square analysis and Fisher's exact tests were used to analyze the data (P< 05). Results No statistically significant difference was found between tooth shade and skin color and the subjects’ perception of their tooth shade. However, a statistically significant difference was found between age and tooth shade (P<.001). There was also a statistically significant difference in tooth shade between men and women. Conclusion Within the limitations of this study, tooth shade value and skin color were not related. However, older adults and men are more likely to have darker teeth. Citation Esan TA, Olusile AO, Akeredolu PA. Factors Influencing Tooth Shade Selection for Completely Edentulous Patients. J Contemp Dent Pract 2006 November;(7)5:080-087.

scholarly journals Validation of Molecular Markers Linked to Fusarium Wilt Resistance in Recombinant Inbred Lines of Chickpea (Cicer arietinum L.)

2021 ◽  
Author(s):  
Dalpat Lal ◽  
Rampura Lakshmipathi Ravikumar ◽  
Pavankumar Jingade ◽  
Sunil Subramanya
Keyword(s):  
Molecular Markers ◽  
Recombinant Inbred ◽  
Mapping Population ◽  
Recombinant Inbred Lines ◽  
Interval Mapping ◽  
Single Marker Analysis ◽  
Wilt Resistance ◽  
Cicer Arietinum L ◽  
Single Marker ◽  
House Condition

Abstract Molecular markers closely linked to Fusarium wilt resistance in chickpea were identified from earlier studies with an objective to validate them. The recombinant inbred line (RIL) mapping population (F11) derived from JG62 (FW susceptible) and WR315 (FW resistant) was used for validation of linked markers. The RILs were phenotyped for FW resistance over two seasons in a wilt sick field at ICRISAT and in wilt sick pots under the green house condition. A total of 42 markers linked to Fusarium wilt (FW) resistance in chickpea were selected from earlier reports for validation. Among 42 markers selected 23 markers were polymorphic among the parental lines of RIL population and hence these markers were used for genotyping the mapping population. The genotyping data together with phenotyping data over two seasons and wilt sick pots identified 12 markers linked to wilt resistance by single marker analysis. The markers TA96, CS27, TA110 and TA59 were more consistently related to FW resistance in this and previous studies. The composite interval mapping has identified two major loci; one each in LG1 and LG2 flanked by markers H4G11 and CaM1402 and H3A12 and CS27, respectively. These two loci are considered as major loci for FW resistance explaining up to 76.66% of phenotypic variation for FW resistance. The results presented here and those published earlier confirm the emerging picture of two hotspots in chickpea genome for resistance to Fusarium wilt. The QTL flanking markers, H4G11, CaM1402, CS27, and H3A12 are most promising and reliable for deployment in chickpea breeding.

PERSEPSI PETERNAK PADA KEPEMIMPINAN PENDAMPING DAN HUBUNGANNYA DENGAN PRODUKTIVITAS USAHA TERNAK DI PAGUYUBAN AL-AWWALIYAH, PASIRWANGI, GARUT, JAWA BARAT

2019 ◽  
Vol 12 (1) ◽  
pp. 42-51
Author(s):  
Dwi Indah Sulistiani ◽  
Ujang Maman ◽  
Junaidi J
Keyword(s):  
Data Analysis ◽  
Secondary Data ◽  
Primary Data ◽  
Leadership Qualities ◽  
Chi Square ◽  
Education Experience ◽  
Spss Software ◽  
Chi Square Analysis ◽  
And Behavior ◽  
The Relationship

Objective of this research; 1) determine the perception of ranchers against the properties and behavior of the leadership of the companion in the Society of Al-Awwaliyah 2) analyze the relationship between productivity breeder with productivity of livestock in the Society of Al-Awwaliyah 3) identify the relationship perceptions of ranchers against the leadership companion with productivity of livestock in the Society of Al-Awwaliyah , The data used in this study are primary and secondary data. Primary data were obtained from questionnaires which stem from ranchers while secondary data sourced from literature in the form of books and articles. Data processing was performed using Chi-square analysis using SPSS software version 21. One of the factors relating to the productivity of ranchers is the perception of ranchers against the leadership of their companion. Leadership companion views of the nature and behavior of which is owned by a companion. Productivity ranchers indirectly related to the productivity of the cattle business. Characteristics breeder visits of age, years of education, experience ranchers, and businesses in addition to ranchers. The results of data analysis showed that there is a significant relationship between business other than ranchers with ranchers productivity. The relationship between the perception of the nature of the companion breeder with productivity ranchers produce Pearson Chi-Square value is 9.751 and Asymp. Sig. (2-sided) of 0.002. This is due to interest ranchers against leadership qualities possessed by a companion who produce prolific ranchers. Ranchers consider that a companion of his leadership qualities are ideal as a companion.

The Effect of Sanctions on Cooperative Behavior: A Study on Medium Trust Individuals in the Context of Corruption

2018 ◽  
Vol 34 (1) ◽  
Author(s):  
Dewi Rosiana ◽  
Achmad Djunaidi ◽  
Indun Lestari Setyono ◽  
Wilis Srisayekti
Keyword(s):  
Logistic Regression ◽  
Public Interest ◽  
Logistic Regression Model ◽  
Cooperative Behavior ◽  
Research Subjects ◽  
Chi Square ◽  
Public Interests ◽  
The Public ◽  
Chi Square Analysis ◽  
Personal Interests

This study aims to describe the effect of sanctions (individual sanctions, collective sanctions, and absence of sanctions) on cooperative behavior of individuals with medium trust in the context of corruption. Both collective sanctions and individual sanctions, are systemic, which means sanctioning behavior is exercised not by each individual but by the system. Cooperative behavior in this context means choosing to obey rules, to reject acts of corruption and to prioritize public interests rather than the personal interests. Conversely, corruption is an uncooperative behavior to the rules, and ignores the public interest and prioritizes personal interests. Research subjects were 62 students. The Chi-Square Analysis was used to see the association between the variables and the logistic regression model was applied to describe the structure of this association. Individual sanction is recommended as punishment to medium trust individuals to promote cooperative behavior in the context of corruption. The results showed that individuals with medium trust had more cooperative behavior.

Evaluation of a New Clinical Test of Fusion Status: A Pilot Study

2019 ◽  
pp. 113-118
Keyword(s):  
Pilot Study ◽  
Ohio State University ◽  
Background Suppression ◽  
Clinical Test ◽  
State University ◽  
Chi Square ◽  
Central Fusion ◽  
Eye Examination ◽  
The Ohio State University ◽  
Chi Square Analysis

Background Suppression is associated with binocular vision conditions such as amblyopia and strabismus. Commercial methods of testing fusion often only measure central fusion or suppression at near. The purpose of this pilot study was to assess a new iPad picture fusion test that assesses foveal and central fusion at near. Methods Participants aged 5 years and older presenting for eye examination at The Ohio State University College of Optometry were enrolled. Results from visual acuity, dry and wet refraction/retinoscopy, stereopsis and cover testing were recorded from the patient chart. The iPad picture fusion test, Worth four-dot, Worth type test with foveal letter targets, and Polarized four-dot were performed by one examiner in a randomized order at 40 cm. Testing was repeated with the anaglyphic filters reversed. Crosstabulation and McNemar chi-square analysis were used to compare the results between fusion testing devices. Results Of the fifty participants (mean age = 17.5), twelve reported suppression and one reported diplopia. Testability was excellent for all tests (98% to 100%). There were no significant differences between tests in reported results (P ≥ 0.22 for all comparisons). No difference in reported fusion or suppression status was observed with change in orientation of the anaglyphic filters. Six participants reported foveal suppression alone at near which was not identified with Worth four-dot at near. Conclusion The iPad picture fusion test provided excellent testability and agreement with commonly used tests of fusion and allowed testing of both central and foveal fusion at near. Nearly half (46%) of participants with suppression reported foveal suppression, supporting the importance of testing for foveal suppression.

PENGARUH PEMBERIAN PELAYANAN INFORMASI OBAT DAN KONSELING TERHADAP TINGKAT KEPATUHAN MINUM OBAT PADA PASIEN DIABETES MELITUS DENGAN HIPERTENSI DI RUMAH SAKIT DAERAH IDAMAN BANJARBARU

2020 ◽  
Vol 5 (2) ◽  
pp. 240-249
Author(s):  
Satrio Wibowo Rahmatullah ◽  
◽  
Ika Maulida Nurrahma ◽  
Adnan Syahrizal
Keyword(s):  
Drug Information ◽  
Treatment Success ◽  
Information Service ◽  
Regional Hospital ◽  
P Value ◽  
Chi Square ◽  
Cross Sectional ◽  
Quasi Experimental ◽  
High Level ◽  
Chi Square Analysis

Compliance to achieve treatment success can be improved by providing drug information services (PIO) and counseling to improve understanding of treatment instructions. The general objective of this study was to determine the effect of drug information service and counseling on the level of medication adherence in patients with diabetes mellitus (DM) with hypertension in the Banjarbaru Regional Hospital. This study uses a quasi experimental method with a cross sectional approach and takes patient data prospectively. The results of this study indicate that the group given PIO and counseling showed a high level of adherence as many as 20 respondents (100%). Whereas in the group that was not given PIO and the counseling level of adherence was low, there were 4 respondents (20%). Based on the mann-whitney analysis p-value = 0,000 (<α = 0.05) so that there are differences in the group with PIO and counseling with groups without PIO and counseling, while the chi square analysis p-value = 0.004 (<α = 0 , 05) so that PIO and counseling have a significant effect on the level of adherence to taking medication in patients with DM with hypertension at the Banjarbaru Regional Hospital.

Trends in the incidence and mortality of oral cancer in Nevada (Preprint)

2020 ◽  
Author(s):  
Kevin Foote ◽  
Karl Kingsley
Keyword(s):  
Public Health ◽  
Oral Cancer ◽  
Cancer Incidence ◽  
Cancer Control ◽  
Cancer Registries ◽  
Public Health Research ◽  
Chi Square ◽  
Incidence And Mortality ◽  
The Us ◽  
Chi Square Analysis

BACKGROUND Reviews of national and state-specific cancer registries have revealed differences in rates of oral cancer incidence and mortality that have implications for public health research and policy. Many significant associations between head and neck (oral) cancers and major risk factors, such as cigarette usage, may be influenced by public health policy such as smoking restrictions and bans – including the Nevada Clean Indoor Act of 2006 (and subsequent modification in 2011). OBJECTIVE Although evaluation of general and regional advances in public policy have been previously evaluated, no recent studies have focused specifically on the changes to the epidemiology of oral cancer incidence and mortality in Nevada. METHODS Cancer incidence and mortality rate data were obtained from the National Cancer Institute (NCI) Division of Cancer Control and Population Sciences (DCCPS) Surveillance, Epidemiology and End Results (SEER) program. Most recently available rate changes in cancer incidence and mortality for Nevada included the years 2012 – 2016 and are age-adjusted to the year 2000 standard US population. Comparisons of any differences between Nevada and the overall US population were evaluated using Chi square analysis. RESULTS This analysis revealed that the overall rates of incidence and mortality from oral cancer in Nevada differs from that observed in the overall US population. For example, although the incidence of oral cancer among Caucasians is increasing in Nevada and the US overall, it is increasing at nearly twice that rate in Nevada, P=0.0002. In addition, although oral cancer incidence among Minorities in the US is declining, it is increasing in Nevada , P=0.0001. Analysis of reported mortality causes revealed that mortality from oral cancer increased in the US overall but declined in Nevada during the same period (2012-2016). More specifically, mortality among both Males and Females in the US is increasing, but is declining in Nevada, P=0.0027. CONCLUSIONS Analysis of the epidemiologic data from Nevada compared with the overall US revealed significant differences in rates of oral cancer incidence and mortality. More specifically, oral cancer incidence increased in Nevada between 2012-2016 among all groups analyzed (Males, Females, White, Minority), while decreases were observed nationally among Females and Minorities. Although mortality in Nevada decreased over this same time period (in contrast to the national trends), the lag time between diagnosis (incidence) and mortality suggests that these trends will change in the near future. CLINICALTRIAL Not applicable

Sign in / Sign up

Export Citation Format

Share Document