Multivariate Analysis and Its Application for Characterizing Cowpea Landraces

Background: Several major crops have been genetically eroded due to introduction of new material but cowpea have great potential and wide genetic base of novel significance. A new strategy of cowpea evaluation was adopted to investigate the potential of 71 landraces representing 31 districts of Pakistan through multivariate analysis to devise future plan for germplasm conservation. Methods: Cowpea 71 landraces collected from 31 districts were planted under field conditions in an augmented design. Plant descriptors qualitative traits (plant color, leaf shape, plant type, twining tendency, anthocynin pigment and fodder types) and agronomic traits (plant height, branches, pods, grain yield, biomass and harvest index) were recorded. Protein banding pattern was established. Similarity index, variance were calculated and phylogenetic trees were constructed by software “Statistica”.Result: Four cowpea landraces showed tremendous performance to phenotypic as well as protein banding pattern variation viz., Pk-27154 (Jhang), Pk-27047 (Vehari), Pk-27029 (Sialkot) and Pk-27107 (Bahawalpur). High inter and intra variation suggested further genotype x environmental interaction trials at multi-locations. Sampling from the neglected areas especially Sindh and Baluchistan parts are hereby recommended to gather unique landraces and accessions before the genetic resources might be depleted through various environmental stresses.

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Efektivitas Metode Seleksi Pedigree dan Modified Bulk pada Tiga Populasi Sorgum (Sorghum bicolor [L.] Moench)

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) ◽

10.24831/jai.v47i3.27661 ◽

2020 ◽

Vol 47 (3) ◽

pp. 275-282

Author(s):

Dewi Andriani ◽

Desta Wirnas ◽

Dan Trikoesoemaningtyas

Keyword(s):

Plant Height ◽

Agronomic Traits ◽

Test Results ◽

Selection Methods ◽

Coefficients Of Variation ◽

Significant Difference ◽

Pedigree Selection ◽

High Heritability ◽

Augmented Design ◽

Materials Used

Sorghum has a growing popularity for food, feed, biofuel, and therefore needs to be developed. The research aimed to compare the effectiveness of pedigree and modified bulk selection methods for improving yield in three sorghum populations. The genetic materials used were 60 F6 lines from three populations, namely PI 10-90-A x Numbu, PI 150-20-A x Numbu and PI 150-20-A x Kawali which had been selected using pedigree and modified bulk methods. The experiment was conducted from October 2018 to February 2019 at the Cikabayan Experimental Station of IPB University, Dramaga, Bogor. The experiment was arranged in an augmented design with six checks, namely Kawali, Numbu, PI 10-90-A, PI 150-20-A, Samurai 1, and Samurai 2 which were replicated four times. Observations were made on agronomic traits and yield. Plant height and grain weight per panicle had high heritability and large genotypic coefficients of variation. The contrast test results showed significant differences between populations in plant height, panicle length, days of harvesting and 100-seed weight. On the other hand, no significant difference was observed among selection methods, indicating that both methods were equally effective for increasing sorghum yield. The pedigree selection and modified bulk selection increased grain yield per panicle by 14.1 g and 18.2 g respectively. Bulk of the best genotypes in early generation could be an alternative of bulk selection method. Keywords: contrast test, differential selection, genotypic coefficient of variation, heritability

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Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton

10.21203/rs.3.rs-17006/v2 ◽

2020 ◽

Author(s):

De Zhu ◽

Ximei Li ◽

Zhiwei Wang ◽

Chunyuan You ◽

Xinhui Nie ◽

...

Keyword(s):

Upland Cotton ◽

Genetic Improvement ◽

Agronomic Traits ◽

Physical Map ◽

Leaf Shape ◽

Interval Length ◽

Phenotypic Variance ◽

Nucleotide Polymorphisms ◽

Substitution Lines ◽

Multiple Alleles

Abstract Background: The low genetic diversity of Upland cotton limits the potential for genetic improvement. Making full use of the genetic resources of Sea-island cotton will facilitate genetic improvement of widely cultivated Upland cotton varieties. The chromosome segments substitution lines (CSSLs) provide an ideal strategy for mapping quantitative trait loci (QTL) in interspecific hybridization.Results: In this study, a CSSL population was developed by PCR-based markers assisted selection (MAS), derived from the crossing and backcrossing of Gossypium hirsutum (Gh) and G. barbadense (Gb), firstly. Then, by whole genome re-sequencing, 11,653,661 high-quality single nucleotide polymorphisms (SNPs) were identified which ultimately constructed 1,211 recombination chromosome introgression segments from Gb. The sequencing-based physical map provided more accurate introgressions than the PCR-based markers. By exploiting CSSLs with mutant morphological traits, the genes responding for leaf shape and fuzz-less mutation in the Gb were identified. Based on a high-resolution recombination bin map to uncover genetic loci determining the phenotypic variance between Gh and Gb, 64 QTLs were identified for 14 agronomic traits with an interval length of 158 kb to 27 Mb. Surprisingly, multiple alleles of Gb showed extremely high value in enhancing cottonseed oil content (SOC). Conclusions: This study provides guidance for studying interspecific inheritance, especially breeding researchers, for future studies using the traditional PCR-based molecular markers and high-throughput re-sequencing technology in the study of CSSLs. Available resources include candidate position for controlling cotton quality and quantitative traits, and excellent breeding materials. Collectively, our results provide insights into the genetic effects of Gb alleles on the Gh, and provide guidance for the utilization of Gb alleles in interspecific breeding.

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The Study of Important Agronomic Traits by Multivariate Analysis in Winter Rapeseed Cultivars

American Journal of Bioscience and Bioengineering ◽

10.11648/j.bio.20140201.13 ◽

2014 ◽

Vol 2 (1) ◽

pp. 15

Author(s):

Gader Ghaffari

Keyword(s):

Multivariate Analysis ◽

Agronomic Traits ◽

Winter Rapeseed

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Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton

10.21203/rs.3.rs-17006/v1 ◽

2020 ◽

Author(s):

De Zhu ◽

Ximei Li ◽

Zhiwei Wang ◽

Chunyuan You ◽

Xinhui Nie ◽

...

Keyword(s):

Upland Cotton ◽

Genetic Improvement ◽

Oil Content ◽

Agronomic Traits ◽

Physical Map ◽

Leaf Shape ◽

Cottonseed Oil ◽

Interval Length ◽

Phenotypic Variance ◽

Multiple Alleles

Abstract Background: The low genetic diversity of Upland cotton limits the potential for genetic improvement. Making full use of the genetic resources of Sea-island cotton will facilitate genetic improvement of widely cultivated Upland cotton varieties. The chromosome segments substitution lines (CSSLs) provide an ideal strategy for mapping quantitative trait loci (QTLs) in interspecific hybridization. Results: In this study, a CSSL population was developed by PCR-based markers assisted selection (MAS), derived from the crossing and backcrossing of Gossypium hirsutum (Gh) and G. barbadense (Gb), firstly. Then, by whole genome re-sequencing, 11,653,661 high-quality single nucleotide polymorphisms (SNPs) were identified which ultimately constructed 1,211 recombination chromosome introgression segments from Gb. The sequencing-based physical map provided more accurate introgressions than the PCR-based markers. By exploiting CSSLs with mutant morphological traits, the genes responding for leaf shape and fuzz-less mutation in the Gb were identified. Based on a high-resolution recombination bin map to uncover genetic loci determining the phenotypic variance between Gh and Gb, 64 QTLs were identified for 14 agronomic traits with an interval length of 158 kb to 27 Mb. Surprisingly, multiple alleles of Gb showed extremely high value in enhancing cottonseed oil content (SOC). Surprisingly, multiple alleles of Gb showed extremely high value in enhancing cottonseed oil content (SOC). Conclusions: This study provides guidance for studying interspecific inheritance, especially breeding researchers, for future studies using the traditional PCR-based molecular markers and high-throughput re-sequencing technology in the study of CSSLs. Available resources include candidate position for controlling cotton quality and quantitative traits, and excellent breeding materials. Collectively, our results provide insights into the genetic effects of Gb alleles on the Gh, and provide guidance for the utilization of Gb alleles in interspecific breeding.

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Morpho-agronomic characterization of sweet potato germplasm

Horticultura Brasileira ◽

10.1590/s0102-053620170410 ◽

2017 ◽

Vol 35 (4) ◽

pp. 534-541 ◽

Cited By ~ 1

Author(s):

Rodrigo P Alves ◽

Arie F Blank ◽

Alisson Marcel S Oliveira ◽

Aléa Dayane D Santana ◽

Vanderson S Pinto ◽

...

Keyword(s):

Sweet Potato ◽

Ipomoea Batatas ◽

Agronomic Traits ◽

Phenotypic Variability ◽

Block Design ◽

Leaf Shape ◽

Dry Mass ◽

Good Prospect ◽

Aboveground Part ◽

Potato Germplasm

ABSTRACT Sweet potato (Ipomoea batatas) spreads mainly by vegetative propagation, and therefore this vegetable may have duplicate accessions in germplasm banks. The authors aimed to characterize morpho-agronomically the sweet potato germplasm from the Active Germplasm Bank (AGB) of Universidade Federal de Sergipe (UFS).The experiment was carried out at the Research Farm “Campus Rural da UFS”. The experimental design was a randomized block design, testing 73 sweet potato accessions with two replicates and four plants per plot. Morpho-agronomic traits of aboveground part and roots were evaluated. We observed that the leaf lobe type, the number of lobes per leaf, shape of central lobe, and general leaf shape were traits which provided the most variability among the accessions. For damage caused by soil insects, 52 accessions showed tolerance. The accessions presented a range from 0.33 to 2.71 t/ha for dry mass of aboveground part and from 1.20 to 10.89 t/ha for the total productivity of the roots. The high phenotypic variability of this crop shows good prospect for breeding programs.

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The Diversity And Classification Of Intraspecies Of Gembolo (Dioscorea Bulbifera L.) Based On Morphological Character

E3S Web of Conferences ◽

10.1051/e3sconf/20187304022 ◽

2018 ◽

Vol 73 ◽

pp. 04022

Author(s):

Andri Prasetia ◽

Purnomo ◽

Budi Setiadi

Keyword(s):

Morphological Character ◽

Leaf Shape ◽

Similarity Index ◽

Morphological Characters ◽

Multivariate Statistical ◽

Pair Group ◽

Group I ◽

Dioscorea Bulbifera ◽

Phenetic Relationship ◽

Special Region

Gembolo (Dioscorea bulbifera L.) is a dioecious, annual, herbaceous, climbing plants and has heart-shaped leaves. Gembolo has aerial tubers (bulbil) and main tuber that has irregular shapes, as well as many rough roots at the base of the stem. The purpose of this research is to know the phenetic relationship of the germplasm of Gembolo in Special Region of Yogyakarta based on morphological character. The results of this study are expected to provide information on intraspecies diversity, phenetic relationship and Gembolo distribution. Gembolo plant samples were taken from D.I Yogyakarta. The sample was observed based on the difference of morphological character, so the number of accessions obtained from D.I Yogyakarta could be determined. Morphological characters would be described and characterized to determine the Operational Taxonomic Units (OTU’s). Based on morphological character data, then the grouping analysis was done by grouping analysis method and the dendrogram was formed by the method of Unweighted Pair Group Methods using Arithmetic averages (UPGMA) using Multivariate Statistical Program (MVSP) software version 3.1pc. The results showed that gembolo had morphological variation in tuber shape, stem color, leaf shape, leaf base shape and leaf tip shape. Based on the morphological characters, 2 main groups with the value of similarity index of 62,8% was formed. Group I consisted of four accessions with a similarity value of 85%. Group II consisted of eight accessions with a similarity value of 75%. A high similarity based on the morphological character on gembolo accession caused the gembolo plants in Special Region of Yogyakarta did not vary.

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CdSe (quantum dots)–graphene oxide system for thiophene polymerization: a new strategy, a new material

RSC Advances ◽

10.1039/c6ra00274a ◽

2016 ◽

Vol 6 (30) ◽

pp. 25577-25583

Author(s):

Edina Rusen ◽

Aurel Diacon ◽

Alexandra Mocanu ◽

Raluca Gavrilă ◽

Leona Cristina Nistor ◽

...

Keyword(s):

Quantum Dots ◽

Graphene Oxide ◽

Cadmium Selenide ◽

Oxidative Polymerization ◽

Oxide System ◽

Cdse Quantum Dots ◽

System P ◽

New Strategy ◽

New Material ◽

Novel Method

This study presents a novel method for the oxidative polymerization of thiophene (T) by employing cadmium selenide (CdSe) quantum dots and CdSe–graphene oxide (GO) as activators of the polymerization system.

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