scholarly journals Étude de la Sensibilité Aux Huiles Essentielles de Cinnamomum Verum, Eucalyptus Globulus, et Glycyrrhiza Glabra L Ainsi qu’aux Antibiotiques de Certains Germes Issus de la Restauration Collective

2018 ◽  
Vol 14 (3) ◽  
pp. 584
Author(s):  
Milan Oba Samoussa ◽  
Abderrazak Abdellaoui ◽  
Anass Kettani ◽  
Rachid Saile ◽  
Houda Bennani
Keyword(s):  
Staphylococcus Aureus ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Eucalyptus Globulus ◽  
Glycyrrhiza Glabra ◽  
Bacterial Strains ◽  
Salmonella Spp ◽  
E Coli ◽  
Zones Of Inhibition ◽  
Glycyrrhiza Glabra L

Food safety is still a very important topic of interest. The use of medicinal plants extracts can be an efficient alternative for fighting food-borne infections in the face of the increase of resistance to antibiotics. We have studied the sensitivity of bacterial strains isolated from food outlets using commonly used antibiotics (Amoxicillin, Vancomycin, Ceftriaxone, Teicoplanin, Rifampicin and Amikacin). This was done using an antibiogram. We have also tested their sensitivity against essential oils extracted from medicinal plants (Cinnamomum verum, Eucalyptus globulus, and Glycyrrhiza glabra L) using aromatogram. This study was conducted using 27 bacterial strains, including 9 Escherichia coli strains, 9 Staphylococcus aureus strains, 9 Salmonella spp. strains, and 3 ATCC strains (E. coli ATCC 25922, Staphylococcus aureus ATCC 25923 et Salmonella typhimurium ATCC 14028). Results revealed that two plant extracts has a substantial antibacterial activity with zones of inhibition ranging from 10 to 25 mm, and it reached 35 mm when using a cocktail of plant extracts. Regarding the antibiotics we used, all strains of Salmonella spp. demonstrated a resistance to amoxicillin and to ceftriaxone. The tested strains of E. coli and Staphylococcus aureus had a partial resistance to the tested antibiotics, which confirms the results of previous studies.

scholarly journals Escherichia coli and Staphylococcus aureus responsible for bovine mastitis sensitivity to the essential oil of Algerian Thymus fontanesii Boiss. et Reut.

2020 ◽  
Vol 7 (1) ◽  
pp. 26-32
Author(s):  
Bendella Amina nor elhouda ◽  
Ghazi Kheira ◽  
Meliani Samia
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Bovine Mastitis ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Zones Of Inhibition

AbstractThe aim of this study is to test two different methods for evaluating the in vitro antibacterial effect of Thymus fontanesii Boiss. et Reut. essential oil against standard and clinical bacterial strains responsible for bovine mastitis: the disc diffusion method or the aromatogram which allows the demonstration of the antibacterial power of essential oils on the bacterial strains tested, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and two strains isolated from bovine mastitis milk S. aureus and E. coli. The inhibition activity of the essential oil of T. fontanesii on bacterial strains by the two methods shows that the antimicrobial power of this oil is very important and is characterized by bactericidal and bacteriostatic action against gram negative and gram positive bacteria. The antimicrobial evaluation by the aromatogram showed good antibacterial activity against all the strains tested, the zones of inhibition of the bacteria were between 23,33±1,527mm and 37,5±3,535 mm. The search for minimum inhibitory concentrations MIC and bactericides CMB made it possible to quantitatively assess the antimicrobial power of this essential oil. In this work, the MIC was 0,625 µl/ml for all strains tested, and the lowest CMB was that of T. fontanesii against E. coli ATCC 25922 was 0,625 µl/ml.

scholarly journals Antibacterial and Antifungal Effect of Eupatorium adenophorum Spreng against Bacterial and Fungal Isolates

1970 ◽  
Vol 10 ◽  
pp. 91-95 ◽  
Author(s):  
Nabin Bhattarai ◽  
Geeta Shrestha
Keyword(s):  
Staphylococcus Aureus ◽  
Organic Solvent ◽  
Plant Extracts ◽  
Antibacterial Effect ◽  
Enterobacter Aerogenes ◽  
Bacterial Strains ◽  
Eupatorium Adenophorum ◽  
Salmonella Spp ◽  
Water Solvent ◽  
Staphylococcus Spp

A research work on antimicrobial effect of water solvent and organic solvent extracts of different concentration of Eupatorium adenophorum (Spreng) was conducted at Nepal Academy of Science and Technology (NAST) during August 2008. Fifteen strains of bacteria, six strains of fungi and two concentrations, 50% and 100% of plant extracts were taken for the study. Among the 15 strains of bacteria, most of them were inhibited with E. adenophorum extracts and only three species, Klebsiella oxytoca, K. pneumoniae and Shigella dysenteriae did not show antibacterial activity with the same extract. Extracts obtained from the organic solvent and water solvent showed different antimicrobial properties with the same bacterial strains. Those bacterial strains whose growth was inhibited by water solvent could not inhibited by organic solvent extracts. Organic solvent extract showed antibacterial effect towards Proteus spp., Salmonella spp., Staphylococcus spp., Bacillus subtilis, B. thurengiensis, B. cereus, Enterobacter aerogenes, Salmonella paratyphi, Staplococcus aureus, Proteus mirabilis and water solvent extract showed antibacterial effect towards Peudomonas aeruginosa, E. coli, Staphylococcus aureus, Staphylococcus spp., Citrobacter frundii, Proteus spp., B. subtilis, B. thurengiensis, Enterobacter aerogenes, Salmonella spp. and S. paratyphi. Altogether 12 species out of 15 were inhibited by E. adenophorum extracts. Both solvent extracts showed high antibacterial effect towards Proteus spp., Staphylococcus spp., Staphylococcus aureus and Pseudomonas aeruginosa. Among the six species of the fungi, Fusarium spp. were inhibited by plant extract while the Aspergillus spp. and Stenophylum botryosum did not show any effect of the extracts. The plant extracts showed selective effect with different strains of bacteria and fungi, which indicated that they were confined to cure the same bacterial diseases and fungal diseases.Key words: Plant extract; Bacteria; Fungi; Antibacterial; Antifungal; Eupatorium adenophorumDOI: 10.3126/njst.v10i0.2834Nepal Journal of Science and Technology Volume 10, 2009 December Page: 91-95

scholarly journals HPLC Analysis of Chemical Composition of Selected Jordanian Medicinal Plants and Their Bioactive Properties

2018 ◽  
Vol 34 (5) ◽  
pp. 2397-2403
Author(s):  
Fuad Al-Rimawi ◽  
Fadi Alakhras ◽  
Wael A. Al-Zereini ◽  
Hammad K. Aldal'in ◽  
Saleh Abu-Lafi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Hplc Analysis ◽  
Room Temperature ◽  
Bacterial Strains ◽  
E Coli ◽  
Bioactive Properties

Three medicinal plants grown wild in Jordan, namely Achillea santolina L, Achillea fragrantisimma, Asteriscus graveolens (Forssk) Less, were extracted with ethyl acetate by continuous shaking at room temperature for three days. The antibacterial activity of the crude extract was evaluated. The extracts were analyzed for their phenolic and flavonoids content by HPLC-PDA. The HPLC analysis of the plant extracts revealed the presence of flavonoids and phenolic compounds in the three plant extracts. Results revealed a strong antibacterial activity of A. graveolens against three bacterial strains (B. subtilis, E. coli, and S.aureus) while A. fragrantissima inhibited the growth of B. subtilis. Bioactivities were attributed mainly to the immense content of phenol-based compounds in plants.

scholarly journals Evaluation of Antimicrobial Efficacy of Ethanol Extracts of Fresh Citrus sinensis (Sweet Orange) Seeds against Selected Bacterial Strains

2020 ◽  
Vol 24 (2) ◽  
pp. 249-252
Author(s):  
E.I. Oikeh ◽  
F.E. Oviasogie ◽  
E.S. Omoregie
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Citrus Sinensis ◽  
Seed Oil ◽  
Inhibitory Concentration ◽  
Antibacterial Activities ◽  
Bacterial Strains ◽  
Salmonella Spp ◽  
Zones Of Inhibition ◽  
Oil Extract

The antibacterial activities of fresh Citrus sinensis seed oil and non-oil  extract are investigated in this study. Gram positive (Staphylococcus aureus and Enterococcus faecalis) and Gram negative (Pseudomonas aeruginosa, Escherichia coli and Salmonella spp.) bacterial strains are used in this study and antibacterial activity is estimated using the agar well diffusion method. Zones of inhibition were measured and the minimum inhibitory concentration and minimum bacteriostatic concentrations were determined. The results show that the non-oil extract had better antibacterial activities against Staphylococcus aureus, Enterococcus faecalis and Escherichia coli compared to the oil extract (zones of inhibition at 200 μg/ml of 12 mm, 8 mm and 2 mm respectively for the nonoil extract; 4 mm, 6 mm, 0 mm respectively for the seed oil). The seed oil however had better antibacterial activity compared to the non-oil extract in Salmonella spp. (zones of inhibition at 200 μg/ml of 8 mm for the seed oil compared to 4 mm for the non-oil extract). Pseudomonas aeruginosa showed similar susceptibility to both extracts at both concentrations studied (zones of inhibition of 4 mm and 2 mm for both extracts at 200 μg/ml and 100 200 μg/ml respectively). Minimum inhibitory concentrations ranged from 50 – 100 μg/ml while minimum bacteriostatic concentrations ranged from 100 – 200 μg/ml. We conclude from our findings that both extracts hold promise in the development of antimicrobial therapeutics. Keywords: Citrus sinensis, Antibacterial, minimum inhibitory concentration, minimum bacteriostatic concentration

scholarly journals The Survey of Microbial Quality of the Dry Sample, Extract and Brewing of some Medicinal Plants

2014 ◽  
Vol 6 (4) ◽  
pp. 478-482
Author(s):  
Razieh VALIASILL ◽  
Majid AZIZI ◽  
Maasome BAHREINI ◽  
Hossein AROUIE
Keyword(s):  
Medicinal Plants ◽  
Pathogenic Bacteria ◽  
Agar Medium ◽  
Total Count ◽  
Plate Count ◽  
Microbial Quality ◽  
Salmonella Spp ◽  
E Coli ◽  
Wide Range

Medicinal plants may be exposed to a wide range of microbial contamination during pre- and post- harvest stages and they can present high microbial counts. In this study, the microbial quality of 44 samples of dry herbs namely: mint (Menthaspp.), lemon balm (Melissa officinalis), summer savory (Satureja hortensis), zataria (Zataria multiflora), Indian valerian (Valeriana wallichii), their brewing and extracts were analyzed. Total count using plate count agar medium (PCA), coliform count by Violet Red Bile Agar (VRBL), Enterobacteriacea by Violet Red Bile Glucose (VRBG) were evaluated. Medium Baird-Parker agar (BP) medium and Tryptone Bile X-Gluc (TBX) medium were used for the isolation and enumeration of Staphylococcus aurous and E. coli spp. respectively. Furthermore, Xylose Lysine Deoxycholate agar medium (XLD) and Bismuth Sulfite Agar medium(BSA) were used for detection of Salmonella spp. Fungal and mold contamination was assessed using yeast extract glucose chloramphenicol agar. The results showed that the contamination of the samples with total count (100%) and Enterobacteriaceae (85%), total coliform (83%), mold and yeast (98%) and E. coli ssp. (2.27) were detected, including in the study samples the absence of pathogenic bacteria like Staphylococcus aurous, Salmonella spp. Moreover, the extract had a lower microbial load in comparison to dry herb samples. Also, the lowest and the highest of contamination rates were observed for Indian valerian and zataria, respectively. According to the results, there is a need to control the environmental conditions and improve hygiene in the production process; even more, it is recommended to choose a suitable decontamination method for disinfection during packing medicinal plants and during post-packing manipulation and transport.

scholarly journals Utilization of Mn-Doped ZnSe/ZnS Core/Shell Quantum Dots for Rapid Detection of Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Thi-Diem Bui ◽  
Quang-Liem Nguyen ◽  
Thi-Bich Luong ◽  
Van Thuan Le ◽  
Van-Dat Doan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Quantum Dots ◽  
Fluorescent Labeling ◽  
Core Shell ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
Methicillin Resistant ◽  
E Coli ◽  
Mn Doped

In this study, Mn-doped ZnSe/ZnS core/shell quantum dots (CSQDs) were synthesized in aqueous solution using polyethylene glycol as a surface stabilizer and successfully applied in the detection of Escherichia coli O157:H7 and methicillin-resistant Staphylococcus aureus (MRSA) for the first time. The CSQDs were conjugated with anti-E. coli antibody and anti-MRSA antibody via protein A supported by 1-ethyl-3-(-3-dimethylaminopropyl)carbodiimide hydrochloride for fluorescent labeling of the intact bacterial cells. The detection was performed for the bacterial strains cultivated in Luria-Bertani liquid medium. The obtained results indicate that E. coli O157:H7 and MRSA can be detected within 30 min at a high sensitivity of 101 CFU/mL. This labeling method based on the highly fluorescent CSQDs may have great potential for use in the food industry to check and prevent outbreaks of foodborne illness.

Antimicrobial Properties of Human Lysozyme Transgenic Mouse Milk

1998 ◽  
Vol 61 (1) ◽  
pp. 52-56 ◽  
Author(s):  
ELIZABETH A. MAGA ◽  
GARY B. ANDERSON ◽  
JAMES S. CULLOR ◽  
WAYNE SMITH ◽  
JAMES D. MURRAY
Keyword(s):  
Staphylococcus Aureus ◽  
Transgenic Mice ◽  
Antimicrobial Properties ◽  
Transgenic Animals ◽  
Average Concentration ◽  
Bacterial Strains ◽  
Human Lysozyme ◽  
Pseudomonas Fragi ◽  
E Coli ◽  
Industry Standard

The antimicrobial properties of standard human lysozyme and the milk of transgenic mice expressing human lysozyme were investigated using bacterial strains important to the dairy industry. Standard human lysozyme was found to be effective at significantly slowing the growth of the milk cold-spoilage organism Pseudomonas fragi (P < 0.001), of a clinical isolate of the mastitis-causing organism Staphylococcus aureus (P < 0.005), and of a nonpathogenic strain of E. coli (P < 0.05). Milk from transgenic mice secreting human lysozyme in their milk at an average concentration of 0.38 mg/ml was found to be bacteriostatic against the cold-spoilage organisms Pseudomonas fragi and Lactobacillus viscous and a mastitis-causing strain of Staphylococcus aureus, but not against a pathogenic strain of E. coli. These results demonstrate that transgenic animals producing human lysozyme in their milk can affect the microbial nature of milk.

Note: Effect of Some Spice Extracts on Bacterial Inhibition

2003 ◽  
Vol 9 (5) ◽  
pp. 353-358 ◽  
Author(s):  
O. Sagdic ◽  
A. G. Karahan ◽  
M. Ozcan ◽  
G. Ozkan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Yersinia Enterocolitica ◽  
Inhibition Zone ◽  
Bacterial Strains ◽  
Inhibitory Effects ◽  
Antibacterial Effects ◽  
E Coli ◽  
Inhibition Zones ◽  
Mrs Broth

Eighteen extracts of spices commonly consumed worldwide and grown naturally in Turkey were tested against twenty three bacterial strains to compare their antibacterial effects with eleven antibiotics. Eight pathogens and fifteen lactobacilli isolated from chick intestine were used as the test microorganisms. Pathogens (six different Staphylococcus aureus strains, Escherichia coli ATCC 25922 and Yersinia enterocolitica ATCC 1501) were grown in Nutrient broth and lactobacilli in MRS broth. Hop extracts formed inhibition zones against S. aureus strains of upto 36 mm. Inhibitory effects of hop extracts against S. aureuswere generally higher than that of erythromycin as antibiotic. Helichrysum compactum extract produced an inhibition zone of 23mm to E. coli ATCC 25922 and 26mm to Y. enterocolitica ATCC 1501. Helichrysum compactum extract inhibited the growth of Y. enterocolitica ATCC 1501 more than other spice extracts. While inhibition zones of these extracts against lactobacilli were found smaller than on S. aureus strains, inhibition zones of the same extracts against lactobacilli were found similar to those of E. coli ATCC 25922 and Y. enterocolitica ATCC 1501.

scholarly journals Growth inhibition of foodborne pathogens in camel milk: Staphylococcus aureus, Listeria monocytogenes, Salmonella spp. and E. coli O157:H7

2017 ◽  
Vol 35 (No. 4) ◽  
pp. 311-320 ◽  
Author(s):  
Abusheliabi Aisha ◽  
Al-Holy Murad A ◽  
Al-Rumaithi Hind ◽  
Al-Khaldi Sufian ◽  
Al-Nabulsi Anas A ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Listeria Monocytogenes ◽  
Growth Inhibition ◽  
Foodborne Pathogens ◽  
Bovine Milk ◽  
Growth Patterns ◽  
Camel Milk ◽  
Salmonella Spp ◽  
E Coli ◽  
Milk Samples

The growth behaviour of foodborne pathogens (Staphylococcus aureus, Listeria monocytogenes, E. coli O157:H7 and Salmonella spp.) was investigated in pasteurised camel milk and compared with pasteurised bovine milk at different incubation temperatures. This study also aimed to compare the growth patterns of these four foodborne pathogens in pasteurised and raw camel milk. Pasteurised or raw camel milk and pasteurised bovine milk were separately inoculated with a cocktail of three strains of each foodborne pathogen. The inoculated milk samples were incubated at 10, 25, and 37°C. The total bacterial count (TBC) in raw milk and the total thermoduric bacteria count (TDB) in pasteurised milk samples were monitored. Greater growth inhibition rates of four pathogens were obtained for the pasteurised camel milk compared to the pasteurised bovine milk. Raw and pasteurised camel milk exerted bacteriostatic effect against all tested pathogens, particularly for the first 8 h of incubation in milk at the different temperatures. Pasteurised camel milk exerted an inhibitory activity that was equivalent to that of raw camel milk.

scholarly journals Bacterial phagocytosis by macrophages from lipopolysaccharide responder and nonresponder mouse strains

1980 ◽  
Vol 28 (3) ◽  
pp. 762-765
Author(s):  
A Cuffini ◽  
N A Carlone ◽  
G Forni
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
The Other ◽  
Mouse Strains ◽  
Bacterial Strains ◽  
Phagocytic Capacity ◽  
Tumoricidal Activity ◽  
E Coli ◽  
Proteose Peptone ◽  
Lipopolysaccharide Endotoxin

The phagocytic capacity of macrophages from C3H/H3J mice was assessed against lipopolysaccharide-producing (Escherichia coli) and -nonproducing (Staphylococcus aureus) bacteria. Despite their gene-coded unresponsiveness to lipopolysaccharide endotoxin and lymphokines and their defective tumoricidal activity, proteose peptone-induced C3H/HeJ macrophages did not display a defective phagocytic capacity, but rather displayed an enhanced phagocytosis of both bacterial strains compared with macrophages from closely related C3H/HeN mice. Unstimulated peritoneal resident C3H/HeJ macrophages, on the other hand, displayed a normal phagocytic activity toward E. coli and enhanced phagocytosis toward S. aureus.

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