scholarly journals Communication Participative dans les Champ Ecole Paysans Pour L’amelioration des Systemes de Cultures Pluviales dans le Departement de Diffa au Niger

2021 ◽  
Vol 17 (39) ◽  
pp. 62
Author(s):  
Rabiou Abdou ◽  
Issaley Nana Aichatou ◽  
Ahmed Lamine ◽  
Mikaillou Souley Issa ◽  
Agbo So Timothée Kouassi ◽  
...  
Keyword(s):  
Participatory Action ◽  
Field Surveys ◽  
Rural Municipality ◽  
Participatory Communication ◽  
Field School ◽  
Rural World ◽  
Level Of Knowledge ◽  
A Site ◽  
Degree Of Participation

La présente étude a pour objectif d’évaluer l’efficacité et le rôle de la communication participative dans les champ école paysans (CEP) mis en œuvre par la Chambre Régionale d’Agriculture (CRA) de Diffa (Niger). La méthodologie utilisée s’est appuyée sur la recherche documentaire, l’observation directe, les enquêtes de terrain auprès des apprenants, des responsables des CEP et des encadreurs (CRA). Au total, 112 personnes, réparties en 96 apprenants, 12 responsables de CEP et 4 encadreurs de CRA, ont été enquêtées sur six sites d’intervention du CRA/Diffa dont trois sites dans la commune urbaine de Diffa, deux sites dans la commune rurale de Gueskerou et un site dans la commune rurale de Chetimari. L’analyse des données a été faite au moyen du logiciel Sphinx V5. Les résultats de l’étude ont montré des taux de l’ordre de 80% à 100% pour le degré de participation au CEP des apprenants, un niveau de connaissance de 100% du thème principal. Il ressort, aussi, des résultats de l’étude un taux allant de 78,12% à 100% de niveau d’adoption des techniques et pratiques agricoles par les apprenants. L’approche CEP, en tant que méthodologie de recherche-action formative et participative, se présente comme une voie convaincante pour faciliter l’adoption et la diffusion des innovations à travers le monde rural. L’originalité et la simplicité du processus permetent non seulement une meilleure appropriation des innovations, mais aussi une participation active des paysans à la production végétale vivrière d‘où une valorisation de la fonction paysanne. The aim of this study was to assess the effectiveness and role of participatory communication in the Farmer Field School (FFS) implemented by the Regional Chamber of Agriculture (RCA) of Diffa (Niger). The methodology used was based on documentary research, direct observation, field surveys of learners, CEP managers and supervisors (CRAs). A total of 112 people divided into 96 learners, 12 FFS managers and 4 RCA supervisors, were surveyed at six RCA / Diffa intervention sites, including three sites in the urban municipality of Diffa, two sites in the rural municipality of Gueskerou and a site in the rural commune of Chetimari. Data analysis is done using Sphinx V5 software. The results of the study showed rates in the order of 80% to 100% for the degree of participation in the FFS of the learners, a level of knowledge of 100% of the main topic. The results of the study also show a rate ranging from 78.12% to 100% of the level of adoption of agricultural techniques and practices by learners. The FFS approach, as a formative and participatory action research methodology, presents itself as a convincing avenue to facilitate the adoption and dissemination of innovations throughout the rural world. The originality and the simplicity of its processes not only allow a better appropriation of innovations, but also an active participation of the farmers in agronomic production and therefore, an enhancement of the farmers function.

The Return of Alsace to France, 1918-1939

2018 ◽  
Author(s):  
Alison Carrol
Keyword(s):  
National Policy ◽  
Third Republic ◽  
French Society ◽  
German Empire ◽  
Cross Border ◽  
The First World War ◽  
German Speaking ◽  
A Site ◽  
First World

In 1918 the end of the First World War triggered the return of Alsace to France after almost fifty years of annexation into the German Empire. Enthusiastic crowds in Paris and Alsace celebrated the homecoming of the so-called lost province, but return proved far less straightforward than anticipated. The region’s German-speaking population demonstrated strong commitment to local cultures and institutions, as well as their own visions of return to France. As a result, the following two decades saw politicians, administrators, industrialists, cultural elites, and others grapple with the question of how to make Alsace French again. The answer did not prove straightforward; differences of opinion emerged both inside and outside the region, and reintegration became a fiercely contested process that remained incomplete when war broke out in 1939. The Return of Alsace to France examines this story. Drawing upon national, regional, and local archives, it follows the difficult process of Alsace’s reintegration into French society, culture, political and economic systems, and legislative and administrative institutions. It connects the microhistory of the region with the macro levels of national policy, international relations, and transnational networks, and with the cross-border flows of ideas, goods, people, and cultural products that shaped daily life in Alsace. Revealing Alsace to be a site of exchange between a range of interest groups with different visions of the region’s future, this book underlines the role of regional populations and cross-border interactions in forging the French Third Republic.

RNA secondary structure dependence in METTL3–METTL14 mRNA methylation is modulated by the N-terminal domain of METTL3

2020 ◽  
Vol 402 (1) ◽  
pp. 89-98
Author(s):  
Nathalie Meiser ◽  
Nicole Mench ◽  
Martin Hengesbach
Keyword(s):  
Secondary Structure ◽  
Rna Binding ◽  
Specific Protein ◽  
Structure Dependence ◽  
Terminal Domain ◽  
Methylation Assay ◽  
A Site ◽  
Methylation Activity

AbstractN6-methyladenosine (m6A) is the most abundant modification in mRNA. The core of the human N6-methyltransferase complex (MTC) is formed by a heterodimer consisting of METTL3 and METTL14, which specifically catalyzes m6A formation within an RRACH sequence context. Using recombinant proteins in a site-specific methylation assay that allows determination of quantitative methylation yields, our results show that this complex methylates its target RNAs not only sequence but also secondary structure dependent. Furthermore, we demonstrate the role of specific protein domains on both RNA binding and substrate turnover, focusing on postulated RNA binding elements. Our results show that one zinc finger motif within the complex is sufficient to bind RNA, however, both zinc fingers are required for methylation activity. We show that the N-terminal domain of METTL3 alters the secondary structure dependence of methylation yields. Our results demonstrate that a cooperative effect of all RNA-binding elements in the METTL3–METTL14 complex is required for efficient catalysis, and that binding of further proteins affecting the NTD of METTL3 may regulate substrate specificity.

scholarly journals The RelA hydrolase domain acts as a molecular switch for (p)ppGpp synthesis

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Anurag Kumar Sinha ◽  
Kristoffer Skovbo Winther
Keyword(s):  
Active Sites ◽  
Molecular Switch ◽  
Cell Physiology ◽  
Synthetase Activity ◽  
Wild Type ◽  
Functional Studies ◽  
Loop Region ◽  
A Site ◽  
Trna Binding

AbstractBacteria synthesize guanosine tetra- and penta phosphate (commonly referred to as (p)ppGpp) in response to environmental stresses. (p)ppGpp reprograms cell physiology and is essential for stress survival, virulence and antibiotic tolerance. Proteins of the RSH superfamily (RelA/SpoT Homologues) are ubiquitously distributed and hydrolyze or synthesize (p)ppGpp. Structural studies have suggested that the shift between hydrolysis and synthesis is governed by conformational antagonism between the two active sites in RSHs. RelA proteins of γ-proteobacteria exclusively synthesize (p)ppGpp and encode an inactive pseudo-hydrolase domain. Escherichia coli RelA synthesizes (p)ppGpp in response to amino acid starvation with cognate uncharged tRNA at the ribosomal A-site, however, mechanistic details to the regulation of the enzymatic activity remain elusive. Here, we show a role of the enzymatically inactive hydrolase domain in modulating the activity of the synthetase domain of RelA. Using mutagenesis screening and functional studies, we identify a loop region (residues 114–130) in the hydrolase domain, which controls the synthetase activity. We show that a synthetase-inactive loop mutant of RelA is not affected for tRNA binding, but binds the ribosome less efficiently than wild type RelA. Our data support the model that the hydrolase domain acts as a molecular switch to regulate the synthetase activity.

scholarly journals Radiology Community Attitude in Saudi Arabia about the Applications of Artificial Intelligence in Radiology

Healthcare ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 834
Author(s):  
Magbool Alelyani ◽  
Sultan Alamri ◽  
Mohammed S. Alqahtani ◽  
Alamin Musa ◽  
Hajar Almater ◽  
...  
Keyword(s):  
Artificial Intelligence ◽  
Saudi Arabia ◽  
Vital Role ◽  
Human Machine Interaction ◽  
The Future ◽  
Level Of Knowledge ◽  
Electronic Questionnaires ◽  
Machine Interaction ◽  
Applications Of Ai

Artificial intelligence (AI) is a broad, umbrella term that encompasses the theory and development of computer systems able to perform tasks normally requiring human intelligence. The aim of this study is to assess the radiology community’s attitude in Saudi Arabia toward the applications of AI. Methods: Data for this study were collected using electronic questionnaires in 2019 and 2020. The study included a total of 714 participants. Data analysis was performed using SPSS Statistics (version 25). Results: The majority of the participants (61.2%) had read or heard about the role of AI in radiology. We also found that radiologists had statistically different responses and tended to read more about AI compared to all other specialists. In addition, 82% of the participants thought that AI must be included in the curriculum of medical and allied health colleges, and 86% of the participants agreed that AI would be essential in the future. Even though human–machine interaction was considered to be one of the most important skills in the future, 89% of the participants thought that it would never replace radiologists. Conclusion: Because AI plays a vital role in radiology, it is important to ensure that radiologists and radiographers have at least a minimum understanding of the technology. Our finding shows an acceptable level of knowledge regarding AI technology and that AI applications should be included in the curriculum of the medical and health sciences colleges.

scholarly journals A MODEL OF CREATIVE HERITAGE FOR INDUSTRY: DESIGNING NEW RULES WHILE PRESERVING THE PRESENT SYSTEM OF RULES

2021 ◽  
Vol 1 ◽  
pp. 141-150
Author(s):  
Honorine Harlé ◽  
Pascal Le Masson ◽  
Benoit Weil
Keyword(s):  
Case Studies ◽  
Design Process ◽  
Industry 4.0 ◽  
Innovation Management ◽  
Present System ◽  
Innovative Capability ◽  
Current Change ◽  
A Site ◽  
Do So

AbstractIn industry, there is at once a strong need for innovation and a need to preserve the existing system of production. Thus, although the literature insists on the necessity of the current change toward Industry 4.0, how to implement it remains problematic because the preservation of the factory is at stake. Moreover, the question of the evolution of the system depends on its innovative capability, but it is difficult to understand how a new rule can be designed and implemented in a factory. This tension between preservation and innovation is often explained in the literature as a process of creative destruction. Looking at the problem from another perspective, this article models the factory as a site of creative heritage, enabling creation within tradition, i.e., creating new rules while preserving the system of rules. Two case studies are presented to illustrate the model. The paper shows that design in the factory relies on the ability to validate solutions. To do so, the design process can explore and give new meaning to the existing rules. The role of innovation management is to choose the degree of revision of the rules and to make it possible.

scholarly journals Crystal structures of Ca2+–calmodulin bound to NaV C-terminal regions suggest role for EF-hand domain in binding and inactivation

2019 ◽  
Vol 116 (22) ◽  
pp. 10763-10772 ◽  
Author(s):  
Bernd R. Gardill ◽  
Ricardo E. Rivera-Acevedo ◽  
Ching-Chieh Tung ◽  
Filip Van Petegem
Keyword(s):  
Crystal Structure ◽  
Binding Sites ◽  
Binding Mode ◽  
Conformational Switch ◽  
Channel Inactivation ◽  
Dependent Inactivation ◽  
Ef Hand ◽  
Inherited Arrhythmia ◽  
A Site

Voltage-gated sodium (NaV) and calcium channels (CaV) form targets for calmodulin (CaM), which affects channel inactivation properties. A major interaction site for CaM resides in the C-terminal (CT) region, consisting of an IQ domain downstream of an EF-hand domain. We present a crystal structure of fully Ca2+-occupied CaM, bound to the CT of NaV1.5. The structure shows that the C-terminal lobe binds to a site ∼90° rotated relative to a previous site reported for an apoCaM complex with the NaV1.5 CT and for ternary complexes containing fibroblast growth factor homologous factors (FHF). We show that the binding of FHFs forces the EF-hand domain in a conformation that does not allow binding of the Ca2+-occupied C-lobe of CaM. These observations highlight the central role of the EF-hand domain in modulating the binding mode of CaM. The binding sites for Ca2+-free and Ca2+-occupied CaM contain targets for mutations linked to long-QT syndrome, a type of inherited arrhythmia. The related NaV1.4 channel has been shown to undergo Ca2+-dependent inactivation (CDI) akin to CaVs. We present a crystal structure of Ca2+/CaM bound to the NaV1.4 IQ domain, which shows a binding mode that would clash with the EF-hand domain. We postulate the relative reorientation of the EF-hand domain and the IQ domain as a possible conformational switch that underlies CDI.

Haemorheological Effects on Thrombocyte Adhesion and Aggregation Found Under Controlled Flow Conditions

1979 ◽  
Author(s):  
P.D. Richardson
Keyword(s):  
Rate Of Change ◽  
Continuous Observation ◽  
Flow Conditions ◽  
Wide Range ◽  
Stream Migration ◽  
Local Shear ◽  
Videotape Recording ◽  
Controlled Flow ◽  
A Site

Thrombocyte adhesion and aggregation in a vessel or on a chamber wall can be measured most readily if the flow is controlled and steady, and continuous observation is used. Videotape recording is very helpful for subsequent quantification of the dynamics. The adhesion of each thrombocyte can occur for a finite time interval:this interval has been observed to have a wide range. Platelets which escape often leave open a site which attracts other platelets preferentially. The rate of change of adhesion density (platelets/mm2) is affected by the local shear rate and the shear history upstream. Aggregation is affected similarly, and also proceeds with some platelet turnover. The role of erythrocytes in facilitating cross-stream migration of thrombocytes (which can enhance the growth rate of large thrombi) appears due in part to convective flow fields induced by the motion of erythrocytes in a shear flow, which can be demonstrated theoretically and experimentally. Observations of the phenomenlogy of adhesion and aggregation under controlled flow conditions and comparison with fLu id-dynamically based theory allows representation in terras of a small number of parameters with prospects of prediction of behaviour over a wide range of haemodynamic conditions; biochemical changes lead to changes in values of the parameters, so that activating agents and inhibiting agents modify values in different directions.

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