scholarly journals The effect of theobromine and NaF 2% exposure to enamel surface hardness after immersing in orange juice beverage

2020 ◽  
Vol 9 (2) ◽  
pp. 70
Author(s):  
Tamara Yuanita ◽  
Setyabudi Setyabudi ◽  
Qintan Sekar Adjani
Keyword(s):  
Orange Juice ◽  
Theobroma Cacao ◽  
Tooth Enamel ◽  
Surface Hardness ◽  
Enamel Surface ◽  
Control Group ◽  
Toxicity Effect ◽  
Group I ◽  
Significant Difference ◽  
Peel Extract

Background: Consumption of orange juice which had acidic quality will cause a demineralization on enamel. The most effective way to prevent the demineralization process was by involving remineralization agents. Fluoride was effective to improve remineralization but has toxicity effect and caused fluorosis at certain dose. Another alternative for remineralization agent without providing side effects is theobromine contained in cocoa peel extract (Theobroma cacao). Objective: To explain the effect of theobromine and NaF 2% exposure on enamel surface hardness after immersion in commercial orange juice. Method: Twenty-seven cattle incisors were cut into a square shape 1x1 cm and then planted in a round resin mold then divided into three groups. All three groups were immersed in orange juice beverage for 60 minutes. The control group was immersed in distilled water, group I was given theobromine 200 mg / L, and group II was given 2% NaF after exposure for 96 minutes. Surface hardness measurements were carried out using the Wolpert Micro Vickers Testers tool. Results: There was a significant difference (p <0.05) between surface hardness of tooth enamel from the control group and theobromine group and there was also a significant difference between the control group and the NaF group. However, there was no significant difference (p> 0.05) in the NaF group with theobromine group. Conclusion: The effect of exposure to theobromine and NaF 2% on surface enamel hardness after immersion in orange juice beverage has the same result.

scholarly journals Applications of Whey Extract and Cpp-Acp in Email Surface Towards Enamel Surface Hardness After Extracoronal Bleaching

2019 ◽  
Vol 6 (2) ◽  
pp. 93-98
Author(s):  
Irfan Dwiandhono ◽  
Dian Noviyanti Agus Imam ◽  
Afiatul Mukaromah
Keyword(s):  
Tooth Enamel ◽  
Artificial Saliva ◽  
Surface Hardness ◽  
Enamel Surface ◽  
Group Iii ◽  
Self Confidence ◽  
Group I ◽  
Significant Difference ◽  
Post Test ◽  
Group Ii

Teeth have an aesthetic value because teeth which are clean, neat and white can increase self confidence. Staining or dental discoloration can disrupt the appearance and cause discomfort. The most common type of discoloration is extrinsic discoloration. Treatment that can be done to overcome extrinsic discoloration is extracoronal bleaching. One way to avoid the extracoronal bleaching side effects is application of remineralization materials. The recommended remineralization materials for post-bleaching are fluoride and phospho peptide-amorphous calcium phosphate (CPP-ACP). This research aims to know the effect of whey extract application and CPP-ACP on tooth enamel hardness after extracoronal bleaching. Experimental laboratory with pre- and post-test control design was performed on 24 first maxillary premolar which devided into 3 groups. On group I, specimens were immersed in artificial saliva. Specimen in group II were immersed in whey extract meanwhile specimens were immersed in CPP-ACP on group III. Whey extract and CPP-ACP immersions were conducted 10 minutes every 12 hours for 15 days. The enamel surface roughness test was performed twice, after extracoronal bleaching treatment and after 15 days remineralization agent application. This study result indicated significant differences between group I and group II and group III (p0,05), but there was no significant difference between group II and group III (p0,05). This study showed the enamel surface hardness after whey extract application is higher than CPP-ACP but difference was not significant statistically

scholarly journals DIFFERENCES IN SURFACE HARDNESS OF BOVINE ENAMEL AFTER EXPOSURE OF THEOBROMINE, ALKALOID COCOA POD EXTRACT AND ACIDULATED PHOSPHATE FLUORIDE

2020 ◽  
Vol 10 (2) ◽  
pp. 71
Author(s):  
Tamara Yuanita ◽  
Nanik Zubaidah ◽  
Jesica Ceren
Keyword(s):  
Vickers Microhardness ◽  
Tooth Enamel ◽  
Surface Hardness ◽  
Enamel Surface ◽  
Control Group ◽  
Group Iii ◽  
Acid Diet ◽  
Round Shape ◽  
Group I ◽  
Bovine Enamel

Background: Acid diet could process demineralization on enamel surface. Process remineralization needed to prevent caries occurrence. The excessive fluorine usage could induce adverse side effects, therefore the development of natural resources such as theobromine and alkaloid cocoa extract as a remineralization is needed. Purpose: To discover the differences of bovine teeth enamel surface hardness after exposure to theobromine, alkaloid cocoa pod extract and acidulated phosphate fluoride. Method: Twenty-eight tooth crowns of bovine insisivus cut into the shape of the square then planted on the resin mold round shape and divided into four groups. The control group without treatment, the group I was exposed APF, the group II was exposed 1000 mg/L alkaloid cocoa pod extract, group III was exposed 200 mg/L Theobromine 200 mg/L. Surface hardness measured using Wolpert Wilson Vickers Microhardness Tool. Results: The group of theobromine produces the highest enamel surface hardness. The enamel surface hardness in alkaloid group of cocoa pod extract was higher compared to the APF group and the control group. Conclusion: The hardness surface of bovine tooth enamel after exposure to theobromine and alkaloid cocoa pod extract is harder than exposure to acidulated phosphate fluoride.

scholarly journals Differences in surface roughness of enamel after whey-extract application and CPP-ACP in post extracoronal-tooth bleaching

2018 ◽  
Vol 4 (1) ◽  
pp. 15
Author(s):  
Afiatul Mukarromah ◽  
Irfan Dwiandhono ◽  
Dian Noviyanti Agus Imam
Keyword(s):  
Surface Roughness ◽  
Artificial Saliva ◽  
Enamel Surface ◽  
Tooth Bleaching ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Group Ii ◽  
Bleaching Treatment

Demineralization is a process of partial or full tooth mineral loss which caused by acidic environment, for example the side effect of extracoronal bleaching treatment. Demineralization increases enamel surface roughness which leads plaque accumulation. Whey extract and calcium phosphopeptide-amorphous calcium phophate (CPP-ACP) contain calcium and phosphate that can stop the demineralization through remineralization process. This study aimed to determine the differences of enamel surface roughness after whey extract and CPP-ACP application post- extracoronal bleaching. Experimental laboratory with pre- and post-test control group design was performed on 24 first maxillary premolars which devided into 3 groups. On group I, specimens were immersed in artificial saliva. Specimens in group II were immersed in whey extract meanwhile specimens were immersed in CPP-ACP on group III. Whey extract and CPP-ACP immersions were conducted 10 minutes every 12 hours for 15 days. The enamel surface roughness test was performed twice, after extracoronal bleaching treatment and after 15 days remineralization agent application. This study result indicated significant differences between group I and group II and between group I and group III (p<0.05), but there was no significant difference between group II and group III (p>0.05). This study showed whey extract decreased more enamel surface roughness than CPP-ACP but the difference was not significant statistically. So, whey extract and CPP-ACP showed similar remineralization potential.

scholarly journals Effect of formula milk on the roughness and hardness of tooth enamel

2021 ◽  
Vol 54 (2) ◽  
pp. 78
Author(s):  
Amaliyah Nur Irianti ◽  
Sri Kuswandari ◽  
Al Supartinah Santoso
Keyword(s):  
Surface Roughness ◽  
Tooth Enamel ◽  
Artificial Saliva ◽  
Enamel Surface ◽  
Formula Milk ◽  
Group Iii ◽  
Treatment Groups ◽  
Group I ◽  
Significant Difference ◽  
Before And After

Background: Demineralisation and remineralisation is a natural process in tooth enamel. It is influenced by the content of calcium and phosphorus in saliva, which concentrations are affected by the consumption of food, including formula milk. Demineralisation and remineralisation determine the roughness and hardness of the enamel surface. Purpose: This study compared the effect of formula milk on the roughness and hardness of tooth enamel. Methods: Maxillary premolar extracted teeth were demineralised with 37% phosphoric acid for 90 seconds and then divided into four treatment groups. For four days, the teeth were immersed twice a day in cow formula for five and ten minutes (Group I and II) and soy formula for five and ten minutes (Group III and IV). Before and after the immersion in milk, the teeth were submerged in artificial saliva. The enamel surface roughness and hardness were measured three times using a surface roughness tester and a Vickers microhardness tester, before and after demineralisation and after immersion in milk. Data were analysed using Kruskal–Wallis and post hoc Mann–Whitney tests. Results: There was no significant difference (p=0.88) observed in the roughness reduction among the treatment groups. The highest increase in hardness was noted for the ten-minute cow formula milk group (93.27 ± 16.00). The increase of hardness was higher after immersion for ten minutes. A substantial difference (p=0.03) was seen in the increase of hardness between the treatment groups. Conclusion: Immersion in cow and soy formula milk for five and ten minutes does not reduce the enamel roughness, but it increases the enamel hardness.

scholarly journals THE EFFECT OF BILIMBI (BELIMBING WULUH) EXTRACT (Averrhoa bilimbi L) AGAINST DENTAL REMINERALIZATION AND ENAMEL MICROSTRUCTURE (IN VITRO RESEARCH)

2017 ◽  
Vol 20 (1) ◽  
pp. 20-26
Author(s):  
Trimurni Abidin ◽  
Marline Nainggolan ◽  
Susi Susi
Keyword(s):  
Tooth Enamel ◽  
Enamel Surface ◽  
Surface Microstructure ◽  
Enamel Microstructure ◽  
Group Iii ◽  
Group I ◽  
High Calcium ◽  
Significant Difference ◽  
The Difference

Teeth physiologically undergo demineralization and remineralisation processes. One way to reduce demineralization can be done with the use of CPP-ACP (Casein Phosphopeptide-Amorphous Calcium Phosphate), which has high calcium bioavailability. The use of natural materials products in the field of dentistry is currently growing, one of which is bilimbi/ starfruit (belimbingwuluh) that is rich in minerals and has many benefits. This study aims to analyze the effect of extract of bilimbi in tooth enamel remineralisation which is tested by using SEM and EDX equipments so that the difference of enamel surface microstructure and the amount of calcium and phosphorus content of tooth enamel surface can be identified. A total of eight hidden molar teeth were extracted, cut into four parts and divided into four groups, namely group I: the teeth applied with the wuluh bilimbi gel, group II: the teeth applied with CPP-ACP gel, group III: the teeth applied with the combination gel of starfruit extract and CPP-ACP gel, group IV: without any application. The result of this study was assessed qualitatively and it was noticeable that the treatment group combination of CPP-ACP gel and starfruit gel had the smoothest enamel surface microstructure. Quantitatively, there were significant difference is the three treatment groups (p≤ 0.05). The conclusion of this research is the effect of the application of wuluh bilimbi extract gel, CPP-ACP gel and gel combination of starfruit with CPP-ACP gel towards the remineralisation and microstructure of tooth enamel surface.

The Toxicity Effect of Echium Amoenum on the Liver and Kidney of Mice.

2020 ◽  
Vol 17 ◽  
Author(s):  
Mozhgan Ghorbani ◽  
Atefeh Araghi ◽  
Nabi Shariatifar ◽  
Seyed Hooman Mirbaha ◽  
Behrokh Marzban Abbasabadi ◽  
...  
Keyword(s):  
Pyrrolizidine Alkaloids ◽  
Biochemical Analysis ◽  
Liver Enzymes ◽  
Copper Ion ◽  
Control Group ◽  
Dependent Manner ◽  
Toxicity Effect ◽  
Significant Difference ◽  
Liver And Kidney ◽  
Echium Amoenum

Aims: The aim of this study was to investigate the toxic effect of Echium amoenum plants on the liver and kidney of animal model. Background: Echium amoenum is one of the medicinal plants containing pyrrolizidine alkaloids with several properties which has widely consumed among different communities. Objective: The toxic effects of Echium amoenum on the liver and kidney were investigated in this study. Methods: Sixty mice were kept for 28 days under the appropriate laboratory conditions. Echium amoenum extract (25, 12.5, 50 mg / kg, ip.) was administered for 28 days. At the end of experiment, blood samples were drawn and liver and kidneys were removed for evaluating hepatotoxicity and nephrotoxicity of extract. Additionally, experiments were conducted to assay the enzymatic and oxidative activities. Results: There was no significant difference in the levels of copper ion in the liver and kidneys among all groups. There was a significant difference in the levels of lipid peroxidation in the liver of treated groups versus control group. The significant difference was not observed in the levels of glutathione of the liver of all groups. However, the levels of glutathione of the kidney significantly decreased in the treated groups versus control group. There was no significant difference in the liver enzymes including ALP, SGOT, and SGPT between all groups. This indicates that damage increase with enhancing the time and concentrations of extract. Biochemical analysis showed the creatinine and urea levels did not change in the treated groups versus control group. Conclusion: According to the present findings, it is suggested that Echium amoenum causes hepatotoxicity and nephrotoxicity effects in dose and time dependent manner.

scholarly journals Regenerative Therapy Modality for Treatment of True Combined Endodontic-Periodontal Lesions: A Randomized Controlled Clinical Trial

2021 ◽  
Vol 18 (12) ◽  
pp. 6220
Author(s):  
Reham AlJasser ◽  
Sundus Bukhary ◽  
Mohammed AlSarhan ◽  
Dalal Alotaibi ◽  
Saleh AlOraini ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Grafting ◽  
Endodontic Treatment ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Bony Defect ◽  
Keratinized Tissue ◽  
Group I ◽  
Significant Difference ◽  
Grafting Procedure

The aim of this in-vivo study was to evaluate/compare the clinical periodontal parameters in patients with true combined endo-perio lesions (EPL), treated with gutta-percha (GP) and mineral trioxide (MTA) as an obturation material alone and with addition of bone grafting in such lesions. 120 Saudi patients (mean age = 41yrs) diagnosed with true combined EPL participated in this study. Group I (control group, n = 30) was treated with conventional endodontic treatment using GP for obturation. Group II (n = 30) was treated with conventional endodontic treatment using MTA for obturation. Group III (n = 30) was treated with conventional endodontic treatment using GP for obturation + grafting procedure to fill the bony defect. Group IV (n = 30) was treated with conventional endodontic treatment using MTA for obturation + grafting procedure to fill the bony defect. Clinical parameters (Pocket depth (PD); Clinical attachment loss (CAL); keratinized tissue width (KTW); gingival phenotype (G.Ph.) and Cone Beam Computed Tomography Periapical Index (CBCTPAI)) were recorded and compared at baseline, 3, 6, 12 months’ interval. For the groups III and IV, CBCTPAI showed significant difference (p < 0.0001) with the other groups at 6 months and 1-year interval. The group with MTA + bone graft showed 76% and 90% patients with 0 score at 6 months and 1-year follow-up, respectively. Comparison of mean values of PD among study groups at 3 months, 6 months and 1 year showed significant difference at 3 months, whereas the mean PD values of subjects in GP + bone graft showed significantly higher PD values than other 3 groups (p = 0.025). Use of GP and MTA for root canal obturation along with periodontal therapy and bone augmentation helps in resolving complex endo-perio lesions. Bone grafting in addition to obturation with MTA was found to be the best treatment strategy in management of EPL cases and is recommended for clinicians who are treating EPL patients.

scholarly journals Comparative Evaluation of Fracture Resistance of Endodontically Treated Teeth Restored with Resin Fiber Post and Stainless Steel Post: An In Vitro Study

2015 ◽  
Vol 03 (02) ◽  
pp. 080-084
Author(s):  
Vijay Singh ◽  
Poonam Bogra ◽  
Saurabh Gupta ◽  
Navneet Kukreja ◽  
Neha Gupta
Keyword(s):  
Stainless Steel ◽  
Fracture Resistance ◽  
Testing Machine ◽  
Compressive Force ◽  
Control Group ◽  
Fiber Post ◽  
Endodontically Treated Teeth ◽  
Group I ◽  
Significant Difference

AbstractFracture resistance of endodontically treated teeth restored with post. Aims: This study aims to compare the fracture resistance of endodontically treated teeth restored with resin fiber and stainless steel post. Commercially available prefabricated resin fiber post(Dentsply Maillefer Easy Post), prefabricated stainless steel post(Coltene/Whaledent Parapost) were used. Methods and Material: Forty five maxillary central incisors were obturated and divided into 3 groups: Control Group (Group I) without any post (n = 15), Resin Fiber Post Group (Group II) (n = 15) and Stainless Steel Post Group (Group III) (n = 15). In all Groups except control group, post space was prepared; a post was cemented, and a core build-up was provided. All the specimens were subjected to compressive force under a universal testing machine until fracture. Statistical analysis used: The results were analyzed using the variable analysis test (ANOVA). Results: One-way analysis of variance revealed significant difference among test groups. The control group demonstrated highest fracture resistance (925.2183 N), followed by the resin fiber post group (486.7265 N) and stainless steel post group (423.539N). Conclusions: Teeth restored with resin fiber post showed higher fracture resistance values than prefabricated stainless steel post.

Histological Study On Using Ileal Submucosa In Repairing Urinary Bladder Defect In Adult Albino Rat

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Eman Gomaa El Saeed ◽  
Manal H Moussa ◽  
Gehad A Hammouda ◽  
Sahar M. M Omar
Keyword(s):  
Urinary Bladder ◽  
Histological Study ◽  
Squamous Metaplasia ◽  
Muscle Layer ◽  
Control Group ◽  
Albino Rats ◽  
Adult Rats ◽  
Group I ◽  
Significant Difference ◽  
Graft Area

Abstract Background Repairing urinary bladder (UB) defect by enterocystoplasty remains the gold standard surgical bladder reconstruction procedure to increase the capacity and compliance of dysfunctional bladders. However, many complications were recorded. Aim of the work This work aimed to compare the consequences of reconstruction of urinary bladder defect using untreated small intestinal submucosal (SIS) matrix versus seeded and unseeded decellularized SIS matrix. Material and Methods Fifty female albino rats were used in this study. The animals were divided into three groups: Group I (Control) included ten adult rats from which ileal tissue was obtained. Group II included ten adult rats in which their UB defect was repaired by untreated cellular SIS. Group III included twenty adult rats that were subdivided into two subgroups, 10 rats each; Subgroup IIIA where rats had their UB defect repaired by acellular SIS and subgroup IIIb where rats had their UB defect repaired by acellular SIS seeded with adipose mesenchymal stem cells (AMSCs).Ten young rats were used for preparation of AMSCs. Morphometric and statistical analysis were also performed. Results In rats where UB defect was repaired by untreated cellular SIS, the graft area showed loss of epithelial polarity, presence of intraepithelial cysts and occasional extension of urothelium to the outer surface forming fistula. There were areas of metaplasia with the appearance PAS positive cells. In the lamina propria, there was areas of lymphocytic infiltration together with significant increase in the collagen fiber deposition (p &lt; 0.05). There was a significant decrease thickness of muscle layer as compared to control (p &lt; 0.05). In rats where UB defect was repaired by acellular SIS, urothelium in the graft area showed occasional squamous metaplasia and often the urothelium extended to the deeper layers forming Brunn's nest. There was minimal muscle regeneration in the graft area. However, in rats where UB defect was repaired by acellular SIS seeded with AMSCs, the urothelium in the graft area was nearly similar to control group with uniform urothelium thickness, minimal collagen fibers deposition and thick muscle layer that showed no significant difference from the control (p &gt; 0.05). Conclusion Acellular SIS seeded with AMSCs showed better results compared to non-seeded and cellular SIS in reconstructing urinary bladder defects.

Impact of supplementing commercial rabbit pellets with fresh Moringa oleifera leaves on semen characteristics in rabbit bucks under tropical climate in Trinidad

2021 ◽  
Author(s):  
Krishna Mohan Kumar
Keyword(s):  
Sperm Motility ◽  
Moringa Oleifera ◽  
Sperm Count ◽  
Control Group ◽  
Computer Assisted ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Moringa Oleifera Leaves ◽  
The Impact

Objective This study aimed to evaluate the impact of the dietary supplement of Moringa oleifera leaves (MOL) on semen quality and characteristics in rabbits. Methods Eighteen (n=18) breeding bucks of New Zealand white, of similar age group, were used for the study. Three feeding regimes, (i) 100% commercial rabbit pellets (CRP)-Group I (ii) 90% CRP + 10% fresh MOL on a dry matter (DM) basis – Group II and (iii) 80% CRP + 20% fresh MOL on a DM basis – Group III, were adopted and the trial continued for 21 days. After adaptation to the diet, semen was collected from each buck and subjected to evaluation using a computer-assisted semen analyser. Results In Group III, the sperm count, normal sperm morphology, and sperm motility increased (52.0%) in comparison with the control (Group I; 50.1%). The inclusion of 20% Moringa oliefera in the diet (Group III) caused a significant increase (P<0.05) in semen concentration (Control =136.2 M/mL; Group III=297.2 M/mL). There was no significant difference (P>0.05) in sperm motility and semen volume among the groups. Conclusion The results suggest that supplementing commercial rabbit pellets with 20% fresh Moringa oliefera leaves on a DM basis can improve the quality and characteristics of semen in breeding bucks.

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