scholarly journals The increase of VEGF expressions and new blood vessels formation in Wistar rats induced with post-tooth extraction sponge amnion

2018 ◽  
Vol 51 (1) ◽  
pp. 1
Author(s):  
Moh. Basroni Rizal ◽  
Elly Munadziroh ◽  
Indah Listiana Kriswandini
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Blood Vessels ◽  
Wistar Rats ◽  
Tooth Extraction ◽  
The Body ◽  
Control Group ◽  
P Value ◽  
Freeze Dried ◽  
Significant Difference

Background: Tooth extraction is the process of removing a tooth from the oral cavity potentially triggering a wound healing response in the body. As a result, many methods have been applied to improve the wound healing process, especially in wounds resulting in complications. One such method involves the application of amniotic membrane which has anti-inflammatory, anti-bacterial, anti-fibrosis, anti-scarring properties with low immunogenicity, epithelialization effects, and secretory leukocyte protease inhibitor (SLPI). It also contains collagen, various growth factors, transferrin, fibronectin, nidogen, proteoglycans, hyaluronan, and laminin. Purpose: This study aimed to determine the effects of sponge amnion on the number of VEGF expressions and new blood vessels in post-tooth extraction wounds of Wistar rats. Methods: Sponge amnion was produced by mixing freeze-dried amnion membrane from the Tissue Bank at RSUD Dr. Soetomo with 1% gelatin before freeze drying the mixture. Wistar rats were then divided into two groups. In Group 1, referred to as the control group, the post-extraction wounds of the rats received no treatment. Meanwhile, in Group 2, the treatment group, the subjects’ post-extraction wounds were treated with sponge amnion. The rats of both groups were sacrificed on day 3 to allow observation of the number of VEGF expressions and new blood vessels. A statistical analysis test, a t-test, was subsequently conducted. Results: There was a significant difference in the number of new blood vessels in the control group and that of the treatment group with a p value of 0.018 (p<0.05). There was also a significant difference in VEGF expression between the two groups with a p value of 0.000 (p <0.05). Conclusion: Sponge amnion can generate a number of VEGF expressions and new blood vessels in the post-extraction wounds of Wistar rats.

scholarly journals Acceleration of post-tooth extraction socket healing after continuous aerobic and anaerobic physical exercise in Wistar rats (Rattus norvegicus)

2020 ◽  
Vol 53 (4) ◽  
pp. 196
Author(s):  
Aqsa Sjuhada Oki ◽  
Moch Febi Alviansyah ◽  
Christian Khoswanto ◽  
Retno Pudji Rahayu ◽  
Muhammad Luthfi
Keyword(s):  
Wound Healing ◽  
Physical Exercise ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Tooth Extraction ◽  
Anaerobic Exercise ◽  
Control Group ◽  
Continuous Training ◽  
Significant Difference ◽  
Aerobic And Anaerobic

Background: Physical exercise has been proven to accelerate wound healing. Physical training itself consists of aerobic (continuous training) and anaerobic (interval training) exercise. The effectiveness of continuous physical exercise on post-tooth extraction wound healing is the focus of this study. Purpose: This study aims to investigate the differences in post-tooth extraction wound healing in Wistar rats (Rattus norvegicus) after aerobic and anaerobic exercise based on the number of fibroblasts and neovascularisation. Methods: Wistar rats were divided into three groups: the control group (K1); K2 undertook continuous aerobic exercise, swimming at 50% maximum swimming capacity (MSC) with an additional 3% bodyweight load; K3 undertook anaerobic continuous exercise, swimming at 65% MSC with a 6% load. The rats swam three times per week for six weeks. The number of fibroblasts and neovascularisation were examined three days after tooth extraction. Data was analysed using the one-way analysis of variance (ANOVA) and Least Significant Difference (LSD) tests (p<0.05). Results: There was a significant difference in the number of fibroblasts between the K2 and K3 groups. There was no significant difference between K2 and K3 in the amount of neovascularisation. Conclusion: There were differences in the number of fibroblasts but not neovascularisation after tooth extraction in Wistar rats given aerobic and anaerobic continuous training.

scholarly journals PENGARUH LUMATAN DAUN PLETEKAN (Ruellia Tuberosa L) TERHADAP LAMA PENUTUPAN LUKA PADA TIKUS PUTIH (Rattus Novergicus)

2017 ◽  
Vol 4 (2) ◽  
pp. 104
Author(s):  
Ria Susana ◽  
Rismia Agustina ◽  
Abdurahman Wahid
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Random Sampling ◽  
Wound Closure ◽  
Simple Random Sampling ◽  
Male Rats ◽  
Control Group ◽  
P Value ◽  
Closure Time ◽  
Significant Difference

ABSTRAKLuka didefinisikan sebagai gangguan pada fungsi dan integritas jaringan tubuh. Penyembuhan luka merupakan proses yang kompleks dari perbaikan dan remodeling jaringan sebagai respon terhadap cedera. Pletekan (ruellia tuberosa l) mengandung flavonoid, glikosida, phenol, saponin dan nutrisi yang diharapkan mempercepat penyembuhan luka. Tujuan penelitian ini adalah mengidentifikasi pengaruh  lumatan daun pletekan (ruellia tuberosa l) terhadap lama penutupan luka pada tikus putih (rattus novergicus). Penelitian ini merupakan studi eksperimental dengan rancangan posttest only control, menggunakan teknik simple random sampling. Sampel yang digunakan sebanyak 40 ekor tikus jantan dengan luka insisi bersih sepanjang 3 cm yang dibagi menjadi 2 kelompok yaitu kelompok kontrol dan kelompok perlakuan. Data yang diukur adalah rata-rata lama penutupan luka. Data yang diperoleh dianalisis menggunakan uji mann-whitney dengan derajat kepercayaan 95% menunjukkan perbedaan yang signifikan rata-rata lama penutupan luka antara kelompok kontrol (8,45) dan kelompok perlakuan (3,75) dengan p-value 0,000 (p<0,05). Kesimpulan penelitian ini adalah terdapat pengaruh lumatan daun  pletekan (ruellia tuberosa l) terhadap lama penutupan luka pada tikus putih (rattus novergicus).Kata-kata kunci: luka insisi bersih, pletekan (ruellia tuberosa l), lama penutupan luka.ABSTRACTWounds is defined as disruption of the function and integrity of body tissues. Wound healing isa complex process of tissue repair and remodelling in response to injury. Pletekan (ruellia tuberosa l) contains flavonoids, glycosides, phenols, saponins and nutritive value that suppose to accelerate wound healing. The purpose of this study was to identify the effect of pletekan leaves (ruellia tuberosa) to wound closure time in rats (rattus novergicus). This research was an experimental study with post- test design using the simple random sampling technique. The samples were 40 male rats with a clean incision wound along 3 cm which were divided into two groups, control group and treatment group. The measured data were the average of wound closure time. Data were analyzed using the mann- whitney test with 95 % confidence level showed a significant difference between the control group (8,45) and the treatment group (3,75) with p-value 0,000 (p<0,005). There was significant effect of ruellia tuberosa l to wound closure time in rattus novergicus.Keywords: wounds, ruellia tuberosa , wound closure time.

scholarly journals Application of pomegranate (Punica granatum Linn.) fruit extract for accelerating post-tooth extraction wound healing

2018 ◽  
Vol 51 (4) ◽  
pp. 189
Author(s):  
Intan Nirwana
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Treatment Group ◽  
Tooth Extraction ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Punica Granatum ◽  
Control Group ◽  
Wound Healing Process ◽  
Significant Difference

Background: Trauma occurring during tooth extraction can cause complications such as bleeding, infection, fracture and dry socket and constitutes an inflammatory response trigger. Pomegranate (Punica granatum Linn.) extract, which contains large amounts of punicallagin and ellagic acid, possesses various qualities, including; anti-inflammatory, anti-bacterial and anti-oxidant. Pomegranate extract can inhibit proinflammatory cytokine production, while also suppressing inflammation response thereby accelerating wound healing. Purpose: This study aimed to analyze the effect of pomegranate extract application to the tooth extraction wounds of Cavia cobaya (C. cobaya) on the expression of fibroblast growth factor-2 (FGF-2) and transforming growth factor β (TGF-β) on the fourth day of the wound-healing process. Methods: This study used 12 C. cobaya, divided into two groups, namely; control and treatment. The subjects were anesthetized, before their lower left central incisor was extracted and the entire socket filled with CMC-Na 3% in members of the control group and pomegranate extract in those of the treatment group. The twelve C. cobaya were sacrificed on day 4, their lower jaw subsequently being removed and decalcified for approximately 30 days. The mandibula tissue was stained using a immunohistochemical technique. FGF-2 and TGF-β were used to evaluate the healing process in the extracted tooth socket. Differences in the expression of FGF-2 and TGF-β were evaluated statistically by means of a t-test. Results: This study indicated a significant difference between the control and the treatment groups (p<0.05). The treatment group members whose sockets were filled with pomegranate extract showed high FGF-2 and TGF-β expression. Conclusion: This study confirmed that the administration of pomegranate extract to post-extraction tooth wounds of C. cobaya increases the expression of FGF-2 and TGF-β on day 4, thereby accelerating the wound healing process.

scholarly journals The Effect of Snakehead Fish (Channa striata) Extract on Inflammation Reaction of Skin Wound Tissue in Rattus novergicus Wistar Strain

2021 ◽  
Vol 33 (1) ◽  
pp. 48
Author(s):  
Nanda Amalia Ramadhanti ◽  
Willy Sandhika ◽  
Agung Dwi Wahyu Widodo
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Blood Vessels ◽  
Inflammatory Reaction ◽  
Skin Wound ◽  
Male Rats ◽  
Control Group ◽  
Significant Difference ◽  
Group 2 ◽  
Wound Tissue

Background: Snakehead fish has been associated with wound healing due to its high albumin content. Albumin can accelerate the inflammatory process so that tissue repair will be faster. Lack of albumin protein causes a person tend to experience prolonged wound healing. Purpose: This study aims to prove the effect of snakehead fish extract on the number of macrophages and blood vessels on the inflammatory reaction of skin wound tissue in rats. Methods: Laboratory experimental design with a total sample size of 27 male rats have undergone incisions, divided into 3 random groups: control group was given aquadest, treatment group 1was given aquadest and 50% extract of snakehead fish, and treatment group 2 was given 100% extract of snakehead fish. The rat’s skin wound tissues were taken on day 5 and observed under the microscope. The average number of macrophages and blood vessels on granulation tissue that formed at the base of the wound were counted and then performed data analysis. Result: In this study, we found a down wards trend in the average number of macrophages and blood vessels in treatment group 1 and treatment group 2. The control group and the treatment group 2 showed a significant difference, however, between the other groups did not show significant difference. Conclusion: There is a significant effect of giving 100% snakehead fish extract on decreased macrophages and blood vessels in the inflammatory reaction of skin wound tissue.

scholarly journals EFEK EKSTRAK MELON (Cucumis melo) dan GliadinTERHADAP KADAR Hb DAN HbCO TIKUS WISTAR JANTAN YANG DIPAPAR ASAP ROKOK

2017 ◽  
Vol 11 (1) ◽  
pp. 78
Author(s):  
Yuyun Erlina Susanti ◽  
Bambang Wirjatmadi
Keyword(s):  
Treatment Group ◽  
Cigarette Smoke ◽  
Cucumis Melo ◽  
Wistar Rats ◽  
Cigarette Smoke Exposure ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

The exposure of cigarette smoke produce CO bond in hemoglobin. Melon (Cucumis melo) and gliadin contains of antioxidants that prevent tissue damage. The aim of this study was to analyze the difference of hemoglobin and carboxyhemoglobin concentration in male Wistar rats were exposed  to cigarette smoke and had been treated melon extract (Cucumis melo) and gliadin. The study was a laboratory experimental design, using Post Test Control Group Design Research and RAL method (Complete Randomize Design). The sample in this study were 25 male Wistar rats aged 3 months. The research was divided into 5 groups with 5 different treatment : control group, the treatment group were given exposure to cigarette smoke, the treatment group were given of cigarette smoke exposure and melon extract (Cucumis melo) and gliadin dose of 3 IU / day, 4.5 IU / day, and 9 IU / day.The subject were divided into 5 groups each treated for 28 day. The sample size used Federer formula. The collection of data was obtained from the results of laboratory tests to hemoglobin and Carboxyhemoglobin. Hb and HbCO data collected and analyzed by Manova test at 95% confidence level. The results showed significant difference in average Hb and HbCO concentration (p-value = 0.000) between male Wistar rats treated and not treated with melon extract (Cucumis melo) and gliadin. Melon extract has an effect on the decline of HbCO concentration due to exposure to cigarette smoke.Keywords:smoke, melon extract, Hb, HbCO

scholarly journals EFFECTS OF COCONUT WATER (COCOS NUCIFERA SP.) ADMINISTRATION AS PREVENTION OF UROLITHIASIS IN CALCIUM OXALATE INDUCED - WHITE RAT WISTAR STRAINS

2021 ◽  
Vol 28 (1) ◽  
pp. 79-90
Author(s):  
Muhammad Reza Gautama ◽  
I Ketut Sudiana ◽  
Soetojo ◽  
Tarmono Djojodimedjo
Keyword(s):  
Renal Function ◽  
Treatment Group ◽  
Calcium Oxalate ◽  
Wistar Rats ◽  
Cocos Nucifera ◽  
Microscopic Analysis ◽  
Coconut Water ◽  
Control Group ◽  
P Value ◽  
Significant Difference

Objective: To analyze the effect of coconut water administration on the occurrence of urolithiasis in calcium oxalate-induced Wistar rats. Material & Methods: Laboratory experimental study with post-test only control group design, using experimental model of male Rattus norwegicus-Wistar strains. The treatment given is ad libitum administration coconut water to observe its effect on renal histopathological changes, microscopic analysis, and renal function markers. Urolithiasis induction uses exposure of 0.75% ethylene glycol (EG), and 2% Ammonium chloride (AC) in drinking water. Results: Descriptive analysis showed the tendency of decreasing in mean of renal function markers, and histopathological nephron damage in the treatment group versus control group: BUN (11.22 versus 14.29); Creatinine Serum (0.5308 versus 0.5714); degree of nephron damage (5.22 versus 10.36). In microscopic analysis, there were calcium deposits in the kidney tubules in the control group, which were not found in the treatment group. Based on the ANOVA test, obtained significant difference in BUN levels with p value=0.023 (p<0.05), and the degree of nephron damage between study groups with p value= 0.000 (p<0.05). There was no significant difference in serum creatinine with p value= 0.23 (p>0.05). Conclusion: Coconut water is potential modality in inhibiting the deposition of calcium oxalate crystals, and protecting from impaired kidney function against urolithiasis in animal-model of calcium oxalate-induced Wistar rats.

scholarly journals Aktivitas Membran Bakiko (Bayam-Kitosan-Kolagen) terhadap Ekspresi Matriks Metaloproteinase-9 pada Luka Bakar Derajat II

2020 ◽  
Vol 7 (1) ◽  
pp. 58
Author(s):  
Hazmi Dwinanda Nurqistan ◽  
Al Munawir ◽  
Ika Rahmawati Sutejo
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Negative Control ◽  
Tissue Growth ◽  
Immunohistochemical Method ◽  
Control Group ◽  
Collagen Membrane ◽  
P Value ◽  
Control Group Design ◽  
Significant Difference

Matrix metalloproteinase-9 (MMP-9) plays an important role in regulating the degradation and deposition extracellular matrix (ECM) that are important for wound reepithelization. However, excessive protease activity can lead to delayed wound healing. Kaemferol in spinach can inhibit the activity of MMP-9. Chitosan and collagen can help tissue growth and have an antimicrobial effect. Chitosan-collagen membranes have been widely used for wound healing but, the results are unsatisfactory. Therefore, the researcher wanted to know the activity of bakiko (Spinach-Chitosan-Collagen) membrane to MMP-9 expression on second degree burn with immunohistochemical method. This research was true-experimental laboratories design with post test only control group design. The experimental animals used were 36 white wistar rats (Rattus novergicus) male, divided into 4 groups ie control group, negative control, positive control (bioplacenton), and treatment (bakiko membrane). Data were obtained by calculating MMP-9 using ImageJ software. The analysis using anova test showed that p value less than 0.05 indicated a significant difference. This study showed that there was a decrease in MMP-9 activity by bakiko membrane in grade II burn with immunohistochemical method in wistar rats.

scholarly journals Expression of fibroblast cells after extraction of wistar rat teeth after topical application of okra fruit (Abelmoschus esculentus) gel

2020 ◽  
Author(s):  
Muhammad Luthfi ◽  
Wisnu Setyari Juliastuti ◽  
Yuniar Aliyah Risky ◽  
Elvina Hasna Wijayanti ◽  
Aisyah Ekasari Rachmawati ◽  
...  
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Wistar Rats ◽  
Tooth Extraction ◽  
Healing Process ◽  
Wistar Rat ◽  
Control Group ◽  
Wound Healing Process ◽  
Fibroblast Cells ◽  
Fruit Extract

Background: Tooth extraction is a dental procedure for removing a teeth from the alveolar bone socket. The tooth extraction process causes damage to hard tissue and soft tissue, so the body will respond physiologically to heal the wound. The wound healing process is divided into several phases, one of which is the proliferation phase of fibroblasts, which is one of the most important phases in the process of wound healing. Okra fruit contains saponins, tannins, flavonoids and alkaloids that have anti-inflammatory, antibacterial, antioxidant effects, and can stimulate angiogenesis so to accelerate the process of wound healing. Objective: to prove that the administration of okra fruit extract can accelerate the process of wound healing after extraction in the teeth of Wistar rats through increased expression of fibroblast cells. Methods: 18 Wistar rats were divided into 2 groups; control group and treatment group. The treatment group received a 30% okra fruit extract. The number of fibroblasts was calculated statistically using One Way ANOVA and Tukey HSD. Results: The results showed that the expression of control group fibroblast cells on day 3 (19.00 ± 2.0), day 5 (21.67 ± 2.08), day 7 (24.00 ± 2.00), whereas in the treatment group on day 3 (24.00 ± 1.00), day 5 (29.00± 2.00), day 7 (30.00 ± 1.53). Anova test between groups showed a significant difference with P-value 0.006. Conclusion: 30% okra fruit extract can increase fibroblast expression in wound healing process after extraction of Wistar rat teeth.

scholarly journals AMBON BANANA PEEL EXTRACT GEL (Musa paradisiaca var. Sapientum (L.) Kunt) ACCELARATES OF WISTAR RATS GINGIVA WOUND HEALING

2021 ◽  
Vol 1 (Volume 1 No 1) ◽  
pp. 89-99
Author(s):  
Florence Meliawaty ◽  
Rina Putri Noer Fadilah ◽  
Putri Mentari
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Wistar Rats ◽  
Healing Process ◽  
Negative Control ◽  
Banana Peel ◽  
Control Group ◽  
Wound Healing Process ◽  
Musa Paradisiaca ◽  
Significant Difference

A wound is a cut through the anatomical structure of tissue due to an incision of a sharp-edged object. Ambon banana peel contains tannins, flavonoids, and saponins which act as an anti-inflammatory antioxidant and antibacterial properties that facilitate the wound healing process. This research attempted to know the effect of ambon banana peel gel extract (Musa paradisiaca var. Sapientum (L.) Kunt) on wound length healing of gingiva mucosa cuts in Wistar rats. The was a pure experimental study with a sample size of 30 Wistar rats divided into three groups; the treatment group was given 10% ambon banana peel gel extract. The positive control group was assigned 10% povidone-iodine, and the negative control group was given aquades. The research was conducted by making an incision on maxillary gingival mucosa 3 mm wide in the horizontal direction with a depth of 0.25 mm using blade number 11. Observations were performed on days 0, 3, 7, and 14 after treatment using a digital calliper. Data were analyzed using the Kruskal Wallis test and then the post hoc test using the Mann Whitney test (p <0.05 significant). The research results showed that the treatment group given 10% ambon banana peel gel extract had a significant difference with a p-value <0.05 compared to the positive and negative control groups on days 3, 7, and 14. The research shows 10%. The concentration of ambon banana peel gel extract affects the accelerating wound healing process in the gingiva mucosa of Wistar rats.

Effect Of Pregnancy Exercise On Third Trimester Primigravida Anxiety In Dealing With Childbirth

2021 ◽  
pp. 76-83
Author(s):  
Ismi Puji Astuti
Keyword(s):  
Treatment Group ◽  
Sampling Technique ◽  
The Body ◽  
Rank Test ◽  
Control Group ◽  
P Value ◽  
Research Subjects ◽  
Third Trimester ◽  
Quasi Experimental ◽  
Signed Rank Test

Background: The first labor anxiety is an unpleasant psychological condition due to physiological changes that cause psychological instability. The condition of excessive anxiety, worry, fear without cause, and stress, causes the muscles of the body to tense up, especially the muscles in the birth path to become stiff and hard, making it difficult to expand. This study analyzed the effect of pregnancy exercise on third trimester primigravida anxiety in dealing with childbirth. Methods: Quasi experimental design with nonrandomized pretest-postest control group. The sampling technique used was purposive sampling with 34 research subjects. 17 people for the treatment group were given pregnancy exercise 8 times, and 17 people for the control group were given health education about preparation for childbirth. Results: The results of data analysis using the Wilcoxon signed rank test showed that the average reduction in the treatment group was 8.00 and the control group was 3.00. The effect of pregnancy exercise on third trimester primigravida anxiety in the treatment group with a p-value (0.000) <(0.05), while the control group with a p-value (0.180)> (0.05). Conclusion: There is an effect of pregnancy exercise on third trimester primigravida anxiety in dealing with childbirth in the Wuryantoro Health Center work area. 

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