scholarly journals Antibacterial potential of Ocimum sanctum oils in relation to Enterococcus faecalis ATCC 29212

2018 ◽  
Vol 51 (3) ◽  
pp. 104
Author(s):  
Diani Prisinda ◽  
Ame Suciati Setiawan ◽  
Fajar Fitriadi
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Ocimum Sanctum ◽  
Clear Zone ◽  
Gram Positive ◽  
Antibacterial Potential

Background: Enterococcus faecalis is a Gram-positive cocci present in the root canal due to the failure of endodontic treatment and  pulp tissue necrosis. The ideal root canal medicine offers biocompatible properties, ease of cleaning, absence of tooth staining and non-disruption of the root canal filling process. Basil (Ocimum sanctum) is one of the herbs widely used in salads which produces anti-bacterial, anti-fungal and anti-viral effects. The antibacterial effect of basil results from the eugenol which represents a main component demonstrating antibacterial properties. Basil essential oil has an antibacterial effect on both gram positive and gram-negative bacteria. Purpose: This study aimed to determine whether the essential oils contained in basil leaves offer any antibacterial potential with regard to the growth of Enterecoccus faecalis ATCC 29212. Methods: The research was experimental in nature incorporating a simple random sampling technique. In this study, groups of active substance compounds contained in basil leaves were extracted by distillation in order to obtain the essential oil. Preparation of the test solution involved essence of basil leaf oil at concentrations of 5,000 ppm, 10,000 ppm and 20,000 ppm in methanol solvent. A phytochemical test of basil was subsequently conducted in order to identify the content of the compound. The bacteria in this study was tested utilizing a disc diffusion method (Kirby and Bauer test) by measuring the diameter of the clear zone (clear zone) which is indicative of the bacterial growth inhibition response of antibacterial compounds in the extract. Results: The results of the research into the phytochemical test showed that basil contains phenolic flavonoids, triterpenoids, saponins, tannins which produce a negative result on steroids. The results of this study showed that the basil essential oil inhibition zone present in the Enterecoccus faecalis growth had a diameter of 11.70 mm at a concentration of 20,000 ppm. This concentration therefore proved most effective in relation to Enterococcus faecalis than other concentrations. Conclusion: It can be concluded that essential oils of basil leaves demonstrate anti-bacterial inhibitory properties with regard to Enterococcus faecalis.

scholarly journals Chemical Indices and Antibacterial Properties of Some Essential Oils of Apiaceae and Lauraceae Spices in Southwest of Algeria

2019 ◽  
Author(s):  
Dalila Razni ◽  
Linda Rouisset ◽  
Elhassan Benyagoub
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Enterococcus Faecalis ◽  
Antibacterial Properties ◽  
Independent Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Cinnamon Essential Oil ◽  
The One

This study is a part of the valorization of extract from three most commonly used Algerian spices, namely; caraway and cumin seeds and cinnamon bark. On the one hand, it aims at characterizing the chemical indices of extracted essential oils and evaluating the antibacterial activity of each essential oil by titration and disc diffusion method respectively. On the other hand, it attempts at evaluating the combined action of essential oils against four reference pathogenic bacterial strains, namely Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis by well and Chabbert-type diffusion method. The essential oils obtained by the hydrodistillation method have a relatively average extraction about 1.43, 2.3 and 2.5%, respectively for caraway, cumin, and cinnamon. The acid index indicates the behavior and amount of free acids present in the essential oil, in which the acid and saponification indices of cinnamon essential oil indicate a value of 4.48 and 168.56 respectively. It can also inform us about the susceptibility of the oil to undergo alterations. The antibacterial activity results showed that cinnamon essential oil (EO) proved to be the most active against the tested bacterial strains; caraway EO was active against Enterococcus faecalis, and the antibacterial action of cumin EO was the lowest. However, the association of the extracted essential oils has a higher synergistic effect than the independent effect of each essential oil, in which the MIC value found was estimated at 10 to 20 (V/V), 40 to 50 (V/V) and 50 to 70 (V/V) respectively for cinnamon, cumin and caraway. The obtained results show that the response to the antibacterial activity varies according to the plant species used and the extract tested alone or in combination.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Baccharis latifolia Pers. and B. Prunifolia H. B. &K. (Asteraceae)

2007 ◽  
Vol 2 (12) ◽  
pp. 1934578X0700201 ◽  
Author(s):  
Janne Rojas ◽  
Judith Velasco ◽  
Luis B. Rojas ◽  
Tulia Díaz ◽  
Juan Carmona ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Enterococcus Faecalis ◽  
Mass Spectra ◽  
Retention Indices ◽  
Gram Positive ◽  
Gram Positive Bacteria

The essential oils from leaves of Baccharis latifolia and B. prunifolia collected in January 2006 were analyzed by GC/MS. The yields of oils extracted by hydrodistillation were 0.27 and 0.29% for B. latifolia and B. prunifolia, respectively. Sixteen (B. latifolia) and twenty nine (B. prunifolia) components were identified by comparison of their mass spectra with the Wiley GC-MS Library data and by their retention indices (RI). The identified products may be divided into four different groups: monoterpenes (9.0% B. latifolia; 43.9% B. prunifolia), oxygenated monoterpenes (0.8% B. latifolia; 5.4% B. prunifolia), sesquiterpenes (20.4% B. latifolia; 45.9% B. prunifolia) and oxygenated sesquiterpenes (69.8% B. latifolia; 1.9% B. prunifolia). The oils showed antibacterial activity only against Gram positive bacteria, with MIC values for Staphylococcus aureus (ATCC 25923) of 80 μg/mL (B. latifolia) and Enterococcus faecalis (ATCC 29212) of 90 μg/mL and 260 μg/mL (B latifolia and B. prunifolia, respectively).

scholarly journals Antibacterial Activity and Chemical Composition of Red Peacock Flower (Caesalpinia pulcherrima L.) Leaf Essential Oil

2019 ◽  
Vol 22 (6) ◽  
pp. 269-274
Author(s):  
Novena Risnalani Rintank Constani ◽  
Hartati Soetjipto ◽  
Sri Hartini
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Leaf Essential Oil

Peacock flower (Caesalpinia pulcherrima L.) leaves contain essential oils which can be used as an ingredient in cosmetics, perfume, aromatherapy, medicine, and supplements. The study was conducted to obtain essential oils from peacock flower leaves and determine the antibacterial activity against gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) and gram-negative (Escherichia coli and Pseudomonas aeruginosa). Antibacterial activity test was carried out by the agar diffusion method, using paper discs. Measurements were made for the inhibition zone diameter (IZD) that appeared, while the essential oil component was analyzed using GC-MS. The results showed that the peacock flower leaves (C. pulcherrima) had a moderate to strong antibacterial effect at a concentration of 7.5%-20% against gram-positive bacteria (B. subtilis and S. aureus) and gram-negative bacteria (E. coli and P. aeruginosa). Gram-negative E. coli bacteria are relatively more sensitive to peacock flower leaf essential oil compared to other test bacteria. Peacock flower (C. pulcherrima) leaf essential oil is composed of 7 main components namely β-Cubebene 33.87%; Caryophyllene 23.00%; γ-Elemene 13.18%; α-Pinene 10.96%; Cadina-1(10),4-diene 10.20%; Copaene; 7.09%; β-Pinene 1.70%.

scholarly journals UJI EFEKTIFITAS ANTIBAKTERI MINYAK ATSIRI SEREH DAPUR SEBAGAI BAHAN MEDIKAMEN SALURAN AKAR TERHADAP BAKTERI ENTEROCOCCUS FAECALIS

e-GIGI ◽  
2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Mario S. Howarto ◽  
Pemsi M. Wowor ◽  
Christy N. Mintjelungan
Keyword(s):  
Essential Oil ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Agar Plate ◽  
Diffusion Method ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Lemongrass Essential Oil

Abstract: Endodontics is the a type of treatment that aims to keep the teeth in function. Disinfection of the root canal is very important in endodontic treatment. Root canal disinfection can be done by giving the material a root canal medication. One of the bacteria that causes the failure of root canal treatment is the bacterium Enterococcus faecalis. The lemongrass essential oil contains geranial, neral, and mirsen which have antimicrobial activity against Gram-positive and Gram-negative. This study aimed to determine whether the lemongrass essential oil was effective against bacteria Enterococcus faecalis. This was an experimental study using post test only control group design with agar plate diffusion method. Samples consisted of a group of lemongrass essential oil with several concentrations: 25%, 50%, 75%, 100%, each consisted of 4 samples. The 16 control groups consisted of positive and negative groups. Diameter of inhibition was determined by the ability to inhibit Enterococcus faecalis cultured on MHA agar. The results showed that the average inhibitory diameter of 25% lemongrass essential oil was 2.60 mm; of 50% was 4.73 mm; of 75% was 4.50 mm; and of 100% was 5.34 mm. Conclusion: Lemongrass essential oil showed an antibacterial effect to inhibit the growth of bacteria Enterococcus faecalis.Keywords: enterococcus faecalis, lemongrass oil, antibacerialAbstrak: Endodontik merupakan salah satu jenis perawatan yang bertujuan mempertahankan gigi agar tetap dapat berfungsi. Disinfeksi saluran akar sangat penting dalam perawatan endodontik. Disinfeksi saluran akar dapat dilakukan dengan memberi bahan medikamen saluran akar. Salah satu bakteri yang menyebabkan kegagalan perawatan saluran akar ialah bakteri Enterococcus faecalis. Minyak atsiri sereh dapur mengandung geranial, neral dan mirsen yang memiliki aktifitas antimikrobapada gram positif dan gram negatif. Penelitian ini bertujuan untuk mengetahui apakah minyak atsiri sereh dapur memiliki efek terhadap bakteri Enterococcus faecalis. Penelitian ini menggunakan metode eksperimental dengan desain post test only control group design dan metode difusi lempeng agar. Sampel penelitian terdiri dari kelompok minyak atsiri sereh dapur dengan konsentrasi 25%, 50%, 75%, dan 100% yang masing-masing terdiri dari 4 sampel; 16 kelompok kontrol terdiri atas kelompok positif dan negatif. Diameter hambat ditentukan berdasarkan kemampuan menghambat Enterococcus faecalis yang dibiakkan pada media agar MHA. Hasil penelitian memperlihatkan rata-rata diameter hambat minyak atsiri sereh dapur dengan konsentrasi 25% sebesar 2,60 mm, 50% sebesar 4,73 mm, 75% sebesar 4,50 mm, dan 100% sebesar 5,34 mm. Simpulan: Minyak atsiri sereh dapur memiliki efek antibakteri untuk menghambat pertumbuhan bakteri Enterococcus faecalis.Kata kunci: enterococcus faecalis, sereh dapur, antibakteri

scholarly journals Efektivitas antibakteri ekstrak daun sirih (Piper betle Linn)terhadap bakteri Enterococcus faecalis (Antibacterial effectiveness of betel leaf extract (Piper Betle Linn) to Enterococcus faecalis)

2014 ◽  
Vol 13 (1) ◽  
pp. 17
Author(s):  
Armianty Armianty ◽  
Indrya Kirana Mattulada
Keyword(s):  
Essential Oils ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Leaf Extract ◽  
Assay Method ◽  
Diffusion Method ◽  
Piper Betle ◽  
Canal Irrigation ◽  
Betel Leaf ◽  
Root Canal Irrigation

Enterococcus faecalis is one of bacteria which have resistance against the pulp tissue defense mechanism and oftenfound in endodontic infections. This bacterial is may hold good colonization, can survive in the root canal withoutother bacteria, and is capable of producing toxins directly or through the induction of inflammation. Betel leaf (Piperbetle Linn)contain essential oils, as the main components of the essential oils are phenols and compounds, amongother derivate such as kavikol compound that has bactericidal five times stronger than phenol. Phenol disrupts threedimensional structure of the bacterial protein to be a random structure and denatured protein, and damages biologicalactivity. Chlorhexidine has been shown to be effective against Enterococcus in the root canal irrigation. Betel leaf canbe chosen as an alternative material root canal irrigation saline. This in vitro experimental laboratory study is aimedto determine the effectiveness of anti-bacterial extracts of betel leaf for E.faecalis bacteria. The minimum inhibitoryconcentration of betel leaf extract is determined by observing the lowest concentration that was first seen clearly,namely 20%. The assay method of these anti-bacterial effect used diffusion method to comparing the inhibition zonebetel leaf extract solution in concentration of 20% be compared 0.2% chlorhexidine, 2% chlorhexidine, and distilledwater. Each group performed eight times repetition respectively. Data analysis used one way anova test followed byLSD test. From this research, it was concluded that 2% chlorhexidine has antibacterial effect for E.faecalis better thanbetel leaf extract and 0.2% chlorhexidine.

scholarly journals Aktivitas Antibakteri Kombinasi Vitamin C dan Amoksisilin sebagai Bahan Alternatif Intrakanal Medikamen terhadap Enterococcus faecalis secara In Vitro

2021 ◽  
Vol 9 (1) ◽  
pp. 60
Author(s):  
Julia Eka Putri Ayuningtyas ◽  
Pudji Astuti ◽  
Dwi Warna Aju Fatmawati
Keyword(s):  
Antibacterial Activity ◽  
Statistical Analysis ◽  
Vitamin C ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Clear Zone ◽  
Alternative Material

Bacteria that are often found in cases of pulp necrosis and root canal treatment are Enterococcus faecalis. Elimination of microorganisms in the root canal can use intracanal medicament. The intracanal medicament can be used from antibiotics such as amoxicillin. The antibacterial power of amoxicillin can be increased by combination with vitamin C to prevent resistance. This research aimed to determine the zone of inhibition of vitamin C and amoxicillin against Enterococcus faecalis. The method used in this study is well diffusion method. Inhibition is shown through the clear zone around the wellbore at 24 hours, 48 ​​hours, 72 hours after incubation. The number of research samples is 16 consisted of 4 groups consisting of amoxicillin, vitamin C, a combination of vitamin C and amoxicillin, and 3 MIX MP. The data were tested by non-parametric statistical analysis Kruskal Wallis and Mann Whitney. The result showed that vitamin C and amoxicillin's antibacterial activity as alternative material intracanal medicament is equivalent to antibacterial 3 MIX MP.

scholarly journals Antibacterial effect of essential oils of two plants Eucalyptus camaldulensis and Artemisia herba alba on some bacterial strains

2021 ◽  
Vol 29 (2) ◽  
pp. 73-77
Author(s):  
H. Fenghour ◽  
H. Bouabida ◽  
D. Dris ◽  
M. Houhamdi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Essential Oil ◽  
Essential Oils ◽  
Antibacterial Effect ◽  
Eucalyptus Camaldulensis ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Plant Metabolites ◽  
Strong Activity ◽  
E Coli

Essential oils are secondary plant metabolites and have many therapeutic properties. The aim of our study is to determine the antibacterial effect of the essential oils of two plants cultivated in a semi-arid region located in the Northeast of Algeria (Tebessa), Eucalyptus camaldulensis (Myrtaceae) and Artemisia herba alba (Asteraceae). The yield of essential oils of the two plants were 1.45 ± 0.026 and 1.21 ± 0.061 g/100 g of the dry matter of the aerial part respectively. The test of the antibacterial effect is based on the diffusion method on solid medium (sensitivity), this method allows us to determine the susceptibility or resistance of an organism vis-à-vis the sample studied. Our study reveals that E. camaldulensis essential oil had very strong activity on all bacterial strains tested, except on Pseudomonas aeruginosa and Enterococcus faecalis for which there was no inhibitory effect. However, A. herba alba essential oil had very strong activity on all bacterial strains tested except on Pseudomonas aeruginosa. The MIC of Artemisia essential oil ranged between 0.08 and 1.57 µL/mL, with the lowest activity for S. aureus and P. mirabilis (1.57 µL/mL) and the highest activity was observed against E. feacalis, E. coli, and K. pneumonia (0.09 µL/mL). The MIC of the second plant EO ranged between 0.08 and 0.36 µL/mL, with the lowest activity for P. mirabilis (0.36 µL/mL) and the highest one was observed against S. saprophyticus and E. coli (0.08 µL/mL). Statistical analysis shows that the two plants have the same efficacy against S. saprophyticus while E. faecalis, K. pneumoniae and P. mirabilis species are affected more by the essential oil of A. herba alba. While, E. camaldulensis has a higher efficiency than that of A. herba alba on the species: S. aureus and E. coli. Therefore, the essential oils of E. camaldulensis and A. herba alba suggests avenues for further non clinical and clinical studies.

scholarly journals Antibacterial activity of essential oil of Juniperus communis L.

2020 ◽  
Vol 18 (1-2) ◽  
Author(s):  
Vesna Kalaba ◽  
Željka Marjanović-Balaban ◽  
Dragana Kalaba ◽  
Dijana Lazić ◽  
Vesna Gojković Cvjetković
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Juniperus Communis ◽  
Herbal Preparations ◽  
Wide Range ◽  
Coagulase Positive Staphylococci

Many herbal raw materials have been used for centuries as a means of treating various types of diseases. Herbal preparations in pharmaceutical forms for oral and external use show a wide range of effects, among which one of the most important antimicrobials. The effect of such herbal preparations on various microorganisms is the subject of many scientific research today, which is mainly fueled by the fact that the high use of antibiotics in more than half a century has led to resistance to microorganisms. Enriched with new results and knowledge of chemistry and the activity of active ingredients derived from plants, today we are experiencing a growing use of herbs in various industries. The Republic of Srpska has a great potential of woody greenery as a possible raw material for the production of essential oils. Therefore, the aim of this study is to define antibacterial effect of juniper berries (Juniperus communis L.) essential oil, obtained by the hydrodestilation process in industrial production conditions. A boiler with capacity of 2000 liters has been used in the hydrodistillation process (hydromodule1:3), the temperature was 102°C, pressure of 0.5 bar, and the time of hydrodistillation process was 2 hours. The yield of essential oil was 1.3 %.The antibacterial effect of the essential oil of the juniper berries was tested using the agar diffusion method on the reference cultures of Salmonella enterica WDCM 00030, Pseudomonas aeruginosa WDCM 00024, Escherichia coli WDCM 00013, Staphylococcus aureus WDCM 00032, Bacilus cereus WDCM 00151- (CEST- Coleccion Espanola De CultivosTipo, Espana), as well as clinical isolates of Providenciastuarti, coagulase positive staphylococci, streptococcus group D and Salmonella spp. from the collection of microbiological laboratory of the Veterinary Institute “Dr Vaso Butozan” Banja Luka.The juniper berries essential oil in a concentration of 20 μl showed antibacterial activity only on the clinical isolate of coagulase-positive staphylococci with an inhibition zone of 12.00 mm, while at a concentration of 100 μl it was shown antibacterial activity on all investigated pathogens (with an inhibition zone of 8.33 mm to 18.00 mm), except on P.aeruginosa and E. coli. The research results indicate to better effect of essential oils compared to reference antibiotics.

Enzyme‐assisted extraction of essential oils from Syzygium aromaticum

2011 ◽  
Vol 1 (6) ◽  
pp. 248-254
Author(s):  
R. Amudan ◽  
D. V. Kamat ◽  
S. D. Kamat
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Steam Distillation ◽  
Antibacterial Effect ◽  
Chemical Extraction ◽  
Syzygium Aromaticum ◽  
Clove Oil ◽  
Zone Size ◽  
Structure Quality ◽  
Assisted Extraction

The essential oil of clove ( Syzygium aromaticum) holds an important positionamongst widely used essential oils. A typical steam distillation processfor the extraction of clove oil provides a 10.1% yield. Recent studies involvedthe use of enzymes such as pectinase, amylase, lignocellulase, and cellulaseon the powder of clove buds, prior to extraction. The traditional methods ofphysical and chemical extraction are effective but may affect the structure,quality and yield of the phytochemicals extracted. In the current study,hence, enzymes specific for action on the cell wall have been used in the pretreatment prior to extraction, to enhance the quality and yield of the phytochemicalsextracted. The results indicated that all the enzymes, gave morethan 50% higher yield than control in terms of weight of extracted essentialoil. A mixture of the enzymes gave the highest yield of 17.82%. Gas chromatographyresults indicated that the essential oil extracted using amylase hada maximum eugenol content of 70%, in comparison with the eugenol content(62–68%) in the essential oils extracted using the rest of the enzymes.Antibacterial activity of all the extracts was studied on methicillin â€resistantStaphylococcus aureus  (MRSA). The essential oil extracted by using amylaseinhibitedMRSA, showed a zone size of 40 mm, whereas the essential oil extractedby using lignocellulase showed a zone size of 45 mm. The gas chromatogramindicated the maximum number of peaks in this extract, whichcould be producing a combined antibacterial effect on the organism. Thespecific gravity values of the essential oil extracted using lignocellulase andamylase was 1.051 and 1.062, respectively, whereas the control had a specificgravity of 1.015.

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