scholarly journals Efektivitas antibakteri ekstrak daun sirih (Piper betle Linn)terhadap bakteri Enterococcus faecalis (Antibacterial effectiveness of betel leaf extract (Piper Betle Linn) to Enterococcus faecalis)

2014 ◽  
Vol 13 (1) ◽  
pp. 17
Author(s):  
Armianty Armianty ◽  
Indrya Kirana Mattulada
Keyword(s):  
Essential Oils ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Leaf Extract ◽  
Assay Method ◽  
Diffusion Method ◽  
Piper Betle ◽  
Canal Irrigation ◽  
Betel Leaf ◽  
Root Canal Irrigation

Enterococcus faecalis is one of bacteria which have resistance against the pulp tissue defense mechanism and oftenfound in endodontic infections. This bacterial is may hold good colonization, can survive in the root canal withoutother bacteria, and is capable of producing toxins directly or through the induction of inflammation. Betel leaf (Piperbetle Linn)contain essential oils, as the main components of the essential oils are phenols and compounds, amongother derivate such as kavikol compound that has bactericidal five times stronger than phenol. Phenol disrupts threedimensional structure of the bacterial protein to be a random structure and denatured protein, and damages biologicalactivity. Chlorhexidine has been shown to be effective against Enterococcus in the root canal irrigation. Betel leaf canbe chosen as an alternative material root canal irrigation saline. This in vitro experimental laboratory study is aimedto determine the effectiveness of anti-bacterial extracts of betel leaf for E.faecalis bacteria. The minimum inhibitoryconcentration of betel leaf extract is determined by observing the lowest concentration that was first seen clearly,namely 20%. The assay method of these anti-bacterial effect used diffusion method to comparing the inhibition zonebetel leaf extract solution in concentration of 20% be compared 0.2% chlorhexidine, 2% chlorhexidine, and distilledwater. Each group performed eight times repetition respectively. Data analysis used one way anova test followed byLSD test. From this research, it was concluded that 2% chlorhexidine has antibacterial effect for E.faecalis better thanbetel leaf extract and 0.2% chlorhexidine.

scholarly journals Efficacy of green tea leaf extract (Camellia sinensis) with NaOCl 2.5% againts Enterococcus faecalis as an alternative solution for root canal irrigation

2016 ◽  
Vol 1 (1) ◽  
pp. 61
Author(s):  
Aries Chandra Trilaksana ◽  
Adeliana Saraswati
Keyword(s):  
Enterococcus Faecalis ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Root Canal ◽  
Leaf Extract ◽  
Alternative Solution ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Tea Leaf ◽  
Bacterial Effect

<p><em>Enterococcus faecalis</em> is a coccal Gram-positive bacteria with facultative anaerob feature. NaOCl have been proven effective againts<em> Enterococcus faecalis</em> for root canal irrigation process. Green tea leaf extract (<em>Camellia sinensis</em>) can be chosen as an alternative solution for root canal irrigation. The purpose of this study is to determine the difference of green tea leaf extract (<em>Camellia sinensis</em>) and NaOCl 2.5% efficacy againts the growth of <em>Enterococcus faecalis</em> bacteria. This is a laboratory experimental study with “post test only group” design. The first step of the trial was done by making green tea leaf extract and then determine the lowest concentration on which the first solution become clear. The concentration that were tested are 1.5%, 2.5%, 3.5%, 4.5% and 5.5%. Based on the trial, it was found that the minimal inhibiton concentration of the green tea leaf extract was 1.5%. The anti-bacterial effect testing method was using diffusion method to differenciate inhibition zone of the green tea leaf extract solution on various concentrations that were being tested and compare it with NaOCl 2.5%. Each group was replicated three times. The obtained data was analyzed with One Way Anova test and the continued with LSD test. The result of the study is that NaOCl 2.5% have superior anti-bacterial effect againts <em>Enterococcus faecalis</em> compared to green tea leaf extract.</p>

scholarly journals Efficacy of green tea leaf extract (camellia sinensis) with NaOCl 2.5% againts enterococcus faecalis as an alternative solution for root canal irrigation

2016 ◽  
Vol 1 (1) ◽  
pp. 58
Author(s):  
Aries C. Trilaksana ◽  
Adeliana Saraswati
Keyword(s):  
Enterococcus Faecalis ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Root Canal ◽  
Leaf Extract ◽  
Alternative Solution ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Tea Leaf ◽  
Bacterial Effect

Enterococcus faecalis is a coccal Gram-positive bacteria with facultative anaerob feature. NaOCl have been proven effective againts Enterococcus faecalis for root canal irrigation process. Green tea leaf extract (Camellia sinensis) can be chosen as an alternative solution for root canal irrigation. The purpose of this study is to determine the difference of green tea leaf extract (Camellia sinensis) and NaOCl 2.5% efficacy againts the growth of Enterococcus faecalis bacteria. This is a laboratory experimental study with “post test only group” design. The first step of the trial was done by making green tea leaf extract and then determine the lowest concentration on which the first solution become clear. The concentration that were tested are 1.5%, 2.5%, 3.5%, 4.5% and 5.5%. Based on the trial, it was found that the minimal inhibiton concentration of the green tea leaf extract was 1.5%. The anti-bacterial effect testing method was using diffusion method to differenciate inhibition zone of the green tea leaf extract solution on various concentrations that were being tested and compare it with NaOCl 2.5%. Each group was replicated three times. The obtained data was analyzed with One Way Anova test and the continued with LSD test. The result of the study is that NaOCl 2.5% have superior anti-bacterial effect againts Enterococcus faecalis compared to green tea leaf extract.

scholarly journals Daya Antibakteri Ekstrak Daun Belimbing Wuluh (Averrhoa bilimbi linn) terhadap Bakteri Enterococcus faecalis (Antibacterial Activity of Averrhoa bilimbi linn Leaf Extract against Enterococcus Faecalis)

2016 ◽  
Vol 6 (2) ◽  
pp. 93
Author(s):  
Ranggi Hardian Nugro Astuti ◽  
Karlina Samadi ◽  
Eric Priyo Prasetyo
Keyword(s):  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Brain Heart Infusion ◽  
Control Group ◽  
Control Group Design ◽  
Canal Irrigation ◽  
Root Canal Irrigation

Background. The prevalence of Enterococcus faecalis bacterial infection caused the failure of root canal treatment between 24%-77%. This is due to various factors resistance and virulence of Enterococcus faecalis. This research to find alternative materials that have antibacterial properties and by utilizing natural ingredients that can later be used as a root canal irrigation. Antibacterial activity of the Averrhoa bilimbi linn leaf extract against Enterococcus faecalis bacteria can be determined by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Purpose. The aim of this study was to determine the concentration of Averrhoa bilimbi linn leaf extract that has antibacterial activity against bacteria Enterococcus faecalis. Method. This research is a labolatory experimental with post test only control group design which use diluted Enterococcus faecalis ATCC 29212 according Mc. Farland standard 1,5 x 108 CFU/ml. With treatment Averrhoa bilimbi linn leaf extract on concentration 50%, 45%, 40%, 35%, 30%, and 25% given to each of 0,05 ml  Enterococcus faecalis and using Brain Heart Infusion Broth (BHIB) as planting media. Result. At the concentration 30% of Averrhoa bilimbi linn leaf extract, showed that colony’s growth less than 10%. At the concentration 35% was not revealed any bacterial growth. Conclusion. The Averrhoa bilimbi linn leaf extract has antibacterial effect on bacteria Enterococcus faecalis. The MIC was at 30% and MBC was at 35%.

The Evaluation of Anti-Microbial-Effect to Enterococcus Faecalis by Hypochlorous Acid Water

2021 ◽  
pp. 1-6
Author(s):  
Yoshishige Yamada
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Colony Formation ◽  
Hypochlorous Acid ◽  
Antimicrobial Effect ◽  
Endodontic Treatment ◽  
High Concentration ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Root Canal Irrigant

Periapical periodontitis is a major disease, and difficult to achieve successful treatment in some cases. Therefore, root canal irrigation is one of the most important processes to successful endodontic treatment. Sodium Hypochlorite (NaOCl) solution is the most commonly used root canal irrigate. However using NaOCl solution on endodontic treatment should be considered its problems such as allergy, serious tissue damage when inadvertently injected into the maxillary sinus or oral mucosa. Therefore, another root canal irrigant that is safe and has a high antimicrobial effect is desirable. In this study, Hypochlorous Acid (HClO) water was used to evaluate the microbicidal efficacy of the Enterococcus faecalis (E. faecalis) and considered the possibility to be a future root canal irrigant candidate. Original (200ppm), 10% diluted (20ppm), 25% diluted (50ppm) and 50% diluted (100ppm) HClO water were used in this study. Each experimental solution was mixed with low concentrations (5×107 CFU/ml) or high concentration (5×109 CFU/ml) of E. faecalis at 30 seconds, 1minute, 3 minutes, 5 minutes respectively. Then they were placed onto BHI agar plates and incubated for 48 h at 37 ºC, followed by counting the number of Colony formation. The results showed that in the condition of 5×107 CFU/ml E.faecalis, no colony formation was shown within 30 seconds by 50 % diluted HClO water. Whereas only original water was attained to inhibit colony formation completely within 30 seconds under the density of 5×109 CFU/ml E, faecalis. In conclusion, 200ppm HClO water has a capacity to anti-microbiological effect against E.faecalis same as NaOCl solution. Keywords: root canal irrigant, hypochlorous acid (HClO) water, Enterococcus faecalis (E. faecalis)

scholarly journals ANTIBACTERIAL EFFECTIVENESS OF LERAK FRUIT ETHANOL EXTRACT (Sapindus rarak DC) AND 2% CHLORHEXIDINE IN Enterococcus faecalis

2021 ◽  
Vol 1 (Volume 1 No 2) ◽  
pp. 188-196
Author(s):  
Ratih Widyasari ◽  
Irfan Fathon ◽  
Rudi Satria Darwis ◽  
Hartanto Endrowahyudi
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Fruit Extract ◽  
Canal Irrigation ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Root Canal Irrigation ◽  
Facultative Anaerobic Bacteria

The failure of a root canal treatment can be caused by the facultative anaerobic bacteria Enterococcus faecalis, which should be eliminated throughout root canal irrigation. Chlorhexidine 2% is a popular antibacterial liquid used in root canal irrigation. Natural substances can also be used to make antibacterial irrigation liquids. One natural substance with antibacterial properties is lerak fruit extract (Sapindus rarak DC), containing 28 per cent of saponins, alkaloid compounds, polyphenols, antioxidant chemicals, and flavonoid groups and tannins. This study was conducted to determine the effectiveness of 25% lerak fruit extract (Sapindus rarak DC) against E. faecalis compared to 2% Chlorhexidine. This study is a pure experimental study using the agar diffusion method against three experimental groups chlorhexidine 2% as a positive control, lerak fruit extract (Sapindus rarak DC) as a treatment group, and aquadest as a negative control. Each group was repeated ten times on Muller Hinton Agar (MHA) media which was applied using a paper disc. Then the diameter of the inhibition zone was calculated using a caliper. The results in this study, 25% lerak (Sapindus rarak DC) fruit extract had an average inhibition zone diameter of 11.775 mm, and an average inhibition zone diameter of 2% chlorhexidine was 19.745 mm. The difference in the diameter of the inhibition zone was significantly different in all groups. Conclusion ethanol extract of lerak fruit (Sapindus rarak DC) 25% can inhibit the growth of E. faecalis and can be used as an alternative to other antimicrobial agents as disinfection of root canal irrigation.

scholarly journals Antibacterial Activity of Leaf Extract of Annona muricata and Simarouba glauca on Enterococcus faecalis

2016 ◽  
Vol 17 (8) ◽  
pp. 650-653 ◽  
Author(s):  
Jain Mathew ◽  
Reshmi George ◽  
Robin Theruvil ◽  
Tobin C Padavil ◽  
Lincy Tomy ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Annona Muricata ◽  
Root Canals ◽  
Root Canal Irrigants ◽  
Simarouba Glauca

ABSTRACT Aim To determine the antimicrobial effect of water extracts of leaves of Annona muricata and Simarouba glauca on Enterococcus faecalis using agar diffusion method. Materials and methods Dried leaves of A. muricata and S. glauca were powdered and extracted in a soxhlet apparatus. Enterococcus faecalis was grown overnight in Trypticase soy agar plates. About 10 μL of each extract was placed on agar plates and incubated overnight. The zone of inhibition was measured after 24 hours. About 1% sodium hypochlorite and distilled water were used as positive and negative controls. Results The leaf extract of A. muricata showed similar effectiveness as that of sodium hypochlorite, whereas the leaf extract of S. glauca showed only a slight reduction in growth of E. faecalis. Conclusion Leaf extract of A. muricata can be developed as an alternative to sodium hypochlorite for root canal irrigants. Clinical significance Success of endodontic treatment depends on complete disinfection of the root canals. Root canal irrigants have a major role in complete disinfection of the root canals. Chemical root canal irrigants are more or less toxic to the oral environment. In this study, naturally derived leaf extracts of A. muricata and S. glauca are compared with sodium hypochlorite for its effectiveness against E. faecalis – the most common pathogen found in the root canals. How to cite this article Mathew J, George R, Theruvil R, Padavil TC, Tomy L, Kurian A. Antibacterial Activity of Leaf Extract of Annona muricata and Simarouba glauca on Enterococcus faecalis. J Contemp Dent Pract 2016;17(8):650-653.

scholarly journals Antibacterial potential of Ocimum sanctum oils in relation to Enterococcus faecalis ATCC 29212

2018 ◽  
Vol 51 (3) ◽  
pp. 104
Author(s):  
Diani Prisinda ◽  
Ame Suciati Setiawan ◽  
Fajar Fitriadi
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Ocimum Sanctum ◽  
Clear Zone ◽  
Gram Positive ◽  
Antibacterial Potential

Background: Enterococcus faecalis is a Gram-positive cocci present in the root canal due to the failure of endodontic treatment and  pulp tissue necrosis. The ideal root canal medicine offers biocompatible properties, ease of cleaning, absence of tooth staining and non-disruption of the root canal filling process. Basil (Ocimum sanctum) is one of the herbs widely used in salads which produces anti-bacterial, anti-fungal and anti-viral effects. The antibacterial effect of basil results from the eugenol which represents a main component demonstrating antibacterial properties. Basil essential oil has an antibacterial effect on both gram positive and gram-negative bacteria. Purpose: This study aimed to determine whether the essential oils contained in basil leaves offer any antibacterial potential with regard to the growth of Enterecoccus faecalis ATCC 29212. Methods: The research was experimental in nature incorporating a simple random sampling technique. In this study, groups of active substance compounds contained in basil leaves were extracted by distillation in order to obtain the essential oil. Preparation of the test solution involved essence of basil leaf oil at concentrations of 5,000 ppm, 10,000 ppm and 20,000 ppm in methanol solvent. A phytochemical test of basil was subsequently conducted in order to identify the content of the compound. The bacteria in this study was tested utilizing a disc diffusion method (Kirby and Bauer test) by measuring the diameter of the clear zone (clear zone) which is indicative of the bacterial growth inhibition response of antibacterial compounds in the extract. Results: The results of the research into the phytochemical test showed that basil contains phenolic flavonoids, triterpenoids, saponins, tannins which produce a negative result on steroids. The results of this study showed that the basil essential oil inhibition zone present in the Enterecoccus faecalis growth had a diameter of 11.70 mm at a concentration of 20,000 ppm. This concentration therefore proved most effective in relation to Enterococcus faecalis than other concentrations. Conclusion: It can be concluded that essential oils of basil leaves demonstrate anti-bacterial inhibitory properties with regard to Enterococcus faecalis.

scholarly journals Comparative Evaluation of the Efficacy of Novel Root Canal Irrigation Techniques on Reduction of Enterococcus faecalis Count: An In Vitro Study

2020 ◽  
Vol 21 (12) ◽  
pp. 1379-1383
Author(s):  
Sabari Murugesan ◽  
N Bharath Naga Reddy ◽  
Buggaveeti Pradeep Kumar ◽  
Purushotham Mohankumar ◽  
Arasappan Rajakumaran ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Comparative Evaluation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Canal Irrigation ◽  
Root Canal Irrigation

scholarly journals Comparison of the Antimicrobial Efficiency of Neem Leaf Extract and 17% Edta with 3% Sodium Hypochlorite against E. faecalis, C. albicans – An in vitro Study

2020 ◽  
pp. 127-136
Author(s):  
T. Santhosh ◽  
S. Delphine Priscilla Antony ◽  
N. P. Muralidharan
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Leaf Extract ◽  
Brain Heart Infusion ◽  
Diffusion Method ◽  
Zones Of Inhibition ◽  
Neem Leaf Extract

Introduction: Candida albicans and Enterococcus faecalis are the most predominant microorganisms found in the canals of failed root canal treated teeth. Thorough debridement of an infected root canal and complete elimination of microorganisms are objectives of effective endodontic therapy. For thousands of years, humans have used herbs as the primary means to sort out health issues and illnesses. Not all herbs have shown to provide scientific evidence as medicine to illness. Neem is a Botanical herb that is truly remarkable with its scientific value, a tropical evergreen native to India. Although different agents have been suggested as root canal irrigants, sodium hypochlorite and EDTA are the most popularly used. Aim: To compare the antimicrobial efficacy of commercially available irrigants, 17% EDTA and a herbal product, neem leaf extract compared with 3% sodium hypochlorite as a control against Enterococcus faecalis and Candida albicans. Materials and Methods: The antimicrobial activity was determined using the agar well diffusion method. Freshly prepared neem leaf extracts, 17% EDTA, 3% Sodium hypochloriteStandard strain of E. Faecalis and C. Albicans were used in this study. These isolates were subcultured on to Brain heart infusion agar (BHI agar) and HI-chrome candidal differential media respectively. The agar well diffusion test was performed in brain heart infusion media and broth. The plates were incubated at 37°C for 24 hours and the zone of inhibition was recorded and analyzed statistically. Results: Zones of inhibition were present with Neem leaf extract suggestive of antimicrobial properties. Zones of inhibition were greater with Neem extract than 17% EDTA. Hence, further research should be directed towards the use of this herbal extract as an irrigant clinically with endodontic therapy. Conclusion: Within the limitation of this study, it was found that neem leaf extract had a significant antimicrobial effect against E. Faecalis and C. Albicans compared to EDTA and Sodium Hypochlorite. Since the study is a qualitative analysis, further testing needs to be done to final quantitative analysis of the antimicrobial activity of neem leaf extract.

scholarly journals Antibacterial activity of Zingiber officinale roscoe extract as a potential root canal irrigation solution against Enterococcus faecalis

2018 ◽  
Vol 30 (2) ◽  
pp. 124
Author(s):  
Rodiyah Azhar ◽  
Elin Julianti ◽  
Setiawan Natasasmita ◽  
Hendra Dian Adhita Dharsono
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Cost Effective ◽  
Zingiber Officinale ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Zingiber Officinale Roscoe ◽  
Irrigation Solution

Introduction: This study was aimed to determine and formulate antibacterial activity of Zingiber Officinale Roscoe (Z. officinale) extract against Enterococcus faecalis (E. faecalis) as a potential irrigating solution which is easily available and cost-effective. Methods:  Extract of Z. officinale Roscoe was yielded through soxhlet technique. Samples divided into two groups, i.e., the extract of Z. officinale Roscoe and chlorhexidine 2% as a control. The antimicrobial activity was observed using the diffuse agar method. Minimum inhibitory concentration (MIC) was determined by serial microdilution method, and minimum bactericidal concentration (MBC) was determined by cultured samples in blood agar. Data were analysed using ANOVA and Kruskal-Wallis test. Results: Extract of Zingiber officinale Roscoe has antimicrobial activity against E. faecalis with the MBC of 15.625 mg/mL. (p<0,05). Conclusion: Extract of Zingiber officinale Roscoe have shown antibacterial activity against E. faecalis and can be further developed as a potential root canal irrigation solution.

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