scholarly journals Technique of Increasing Calcium Carbonate (CaCO3) in White Shrimp (Litopenaeus vannamei) Feed in Kasetsart University, Bangkok

2021 ◽  
Vol 10 (3) ◽  
pp. 118
Author(s):  
Elva Nurfaidah ◽  
Agustono Agustono

White shrimp (Litopenaeus vannamei) culture has grown rapidly in Indonesia since  early 2000s. White shrimp have stronger resistance to WSSDV attacks, and are euryhaline. In it’s natural habitat, this shrimp was found in waters with a range of 0.5-40 ppt salinity. White shrimp grow optimally at 15-25 ppt salinity, even still suitable at 5 ppt salinity. This capability provides the opportunities to culture these commodities in inland water. White shrimp need inorganic elements or minerals for their living processes. Calcium is one of the main needs of shrimp that is closely related to the development and maintenance of skeletal systems and plays a role in some physiological processes of the body.The requirement for calcium in shrimp is increasing when it is cultivated in fresh water.This Internship aims to find out the technique of adding calcium carbonate (CaCO3) in feed, used for the white shrimp culture in fresh water. In this field practice, the addition of calcium carbonate in a level of 2% refers to the requirement of white shrimp for optimal growth. This was based on the calcium requirements of shrimp in the amount of 1-2%. The method was repelleting, by grinding feed and calcium using mortar and pestle, then filtered to get the smoothest part. Then mix calcium with 10 mL of water and mix it with pellets evenly. Then the mixture is air dried and mashed again and weighed according to the required dose of feed. The addition of calcium in the feed affects the growth of shrimp, because calcium is one of the most important anorganic mineral for the growth and development of exoskeleton, regulates blood clotting, heart rate, kidneys, nerves, the activity of several enzymes, and cell function

2007 ◽  
Vol 5 (2) ◽  
pp. 179
Author(s):  
. Sukenda ◽  
P. Hadi ◽  
E. Harris

<p>Disinfection and nutrient enrichment prior stocking of  post larvae in the pond will be affected on the growth and composition of microbe.  Attention should be taken to some factors related to deterministic and stochastic factors of aquaculture environment  in order to develop microbe community.  This study was performed to determine effect of sucrose and probiotic supplementation to shrimp culture pond on water quality profile and population dynamic on shrimp culture media.  The treatments were supplementation of sucrose as carbon source, probiotic, and sucrose + probiotic into 25 L culture medium containing white shrimp, <em>Litopenaeus vannamei</em>.  Shrimp were fed commercial diet containing 30% protein by 5% body weight every day.  The result of study showed that bacterial population was increased by increasing time of shrimp rearing.  Increased of bacterial population was contrary to DO value.   Bacteria grew was heterotrop and vibrio that its intensity varied during experiment.  Supplementation of sucrose supported proliferation of bacteria including heterotrop, probiotik and vibrio groups.  Specifically, interaction between probiotic bacteria and vibrio was also found.  The presence of probiotic bacteria showed a negative impact on vibrio population.  Further, development of bacteria in general was also implicated to fluctuation of ammonia concentration in pond.</p> <p>Keywords: carbon, sucrose, probiotic, white shrimp, <em>Litopenaeus vannamei</em></p> <p> </p> <p>ABSTRAK</p> <p>Kegiatan disinfeksi dan pengkayaan nutrien sebelum penebaran PL akan mempengaruhi pola pertumbuhan dan komposisi mikroba di tambak. Hal-hal yang perlu diperhatikan dalam pengembangan komunitas mikroba adalah faktor-faktor <em>deterministic</em> dan <em>sthocastic</em> masing-masing lingkungan budidaya. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian bahan berkarbon (sukrosa) dan probiotik di tambak terhadap profil kualitas air serta dinamika populasi pada perairan budidaya. Pada penelitian ini dilakukan penambahan sumber karbon (sukrosa), penambahan probiotik dan penambahan sukrosa + probiotik pada masing-masing wadah yang berisi 25 liter air dan udang Vanamei, <em>Litopenaeus vannamei</em>. Pakan yang diberikan berupa pellet komersial dengan kadar protein 30% setiap hari sebanyak 5% dari biomassa awal. Dari hasil penelitian ini diketahui bahwa populasi bakteri pada media budidaya meningkat seiring dengan bertambahnya waktu pemeliharaan. Peningkatan jumlah populasi bakteri ini diikuti oleh semakin menurunnya nilai DO secara umum. Selain bakteri heterohof, tumbuh juga bakteri golongan <em>Vibrio</em> dengan persentase kemunculan yang berbeda pada setiap perlakuan. Penambahan sukrosa dalam media budidaya mendukung proliferasi bakteri secara umum, heterotrof, probiotik dan golongan <em>Vibrio</em>. Secara spesifik, timbul juga interaksi antara bakteri probiotik dengan bakteri <em>Vibrio</em>. Tumbuhnya bakteri probiotik berimplikasi negatif terhadap populasi <em>V</em><em>i</em><em>brio</em>. Selain itu pertumbuhan bakteri secara umum juga berimplikasi terhadap fluktuasi kadar amonia perairan.</p> <p>Kata kunci : karbon, sukrosa, probiotik, udang vaname, <em>Litopenaeus vannamei</em></p>


Author(s):  
K. Sirisha ◽  
Y. Aparna ◽  
M. Srinivasulu Reddy

Shrimp culture has been developed during the last three decades and the production of farmed shrimp reached its peak. However, the shrimp production was decreased all over the World including in Asian Countries because of the mass mortality due to the outbreak of several diseases predominantly White Spot Syndrome Virus (WSSV) which caused extensive economic damage to the shrimp culture industry. Various Polyculture technologies of shrimp with shellfish, finfish or seaweeds have been implemented to reduce economic damages by mass mortality of shrimp. The present study was conducted with Tiger shrimp Penaeus monodon and Pacific white shrimp Litopenaeus vannamei through Polyculture operation to determine optimum stocking ratios for induction of best performance and highest production rates. Initially, the experiments were conducted with different ratios and combinations of stocking densities 1:1, 2:1 and 1:2 ratios of Penaeus monodon and Litopenaeus vannamei with control group feed with commercial feed, the other group certain Probiotic bacteria such as Bacillus licheniformis and Lactobacillus rhamnosus added in feed, the third group both the Probiotic bacteria and the addition of external Carbon source from sugarcane molasses in the form of Bioflocs for 100 days. To ascertain further interaction of ratios influence, 20:10, 20:12, 20:15 ratios of stocking and Monoculture experiments were conducted. In both the experiments the Survival rate, Final body weights, Weight Gain, Feed conversion ratio, Specific growth rates, Average daily growth rates, Protein efficiency ratio, and Productivity rates were significantly different (P<0.05) in all the different treatments both for P. monodon and L. vannamei. From the results obtained 20:10 ratio of P. monodon and L. vannamei was considered as the best ratio of stocking, which yielded the highest production rates in all the Control, Probiotic added and both Probiotic & Biofloc added groups. The results obtained in the present study clearly demonstrate that the rearing of taxonomically similar species with optimum stocking ratios seems to improve the efficiency of shrimp farming and substantially increasing the production rates. So, this polyculture of P. monodon and L. vannamei can be considered as an alternative approach towards the establishment of sustainable shrimp farming activity which will yield good economic returns.


2016 ◽  
Vol 8 (1) ◽  
pp. 403-417
Author(s):  
Irzal Effendi ◽  
Muhammad Agus Suprayudi ◽  
I Wayan Nurjaya ◽  
Enang Harris Surawidjaja ◽  
Eddy Supriyono ◽  
...  

The purposes of this study were to determine oceanographic and water quality parameters and their suitability for white shrimp Litopenaeus vannamei culture. The measurements were carried out on dry season in Semak Daun Island, Karya Island, and Panggang Island waters of Thousand Islands, with areas of 315.0, 12.0, and 102.8 ha, water depth average of 4.6 m (0.5-28.1 m), 14.6 m (0.5-26.7 m), and 5.3 m (0.8-13.6 m), mean current water velocity of  12.9, 12.7, and 13.5 cm/second, respectively.  In the study areas, we found a diurnal tidal pattern with high wave in January and July-August.  Based on temperature, salinity, and water density in Semak Daun Island waters, there seemingly occurred a turn over indicating a good water circulation, while in Panggang Island and Karya Island waters tended to have a stratification. Generaly, water qualities in the study areas were in the op-timum range for white shrimp culture, i.e., temperature of 29.6-30.8oC, turbidity of 0.10-1.05 NTU, transparency of 5.8-9.7 m, total suspended solid of <8 mg/L, total dissolved solid of 20-164 mg/L, pH of 6.89-7.22, salinity of 32.2-32.3, dissolved oxygen of 5.8-10.8 mg/L, ammonia of 0.068-0.145 mg/L, nitrate 1.247-2.589 mg/L, and phosphate  of 1.021-2.352 mg/L. Moreover, in Semak Daun Island wa-ters, we found the highest suitability for white shrimp culture due to its better water circulation.Keywords: mariculture, coral reef waters, strait, water current, turnover, stratification.


2021 ◽  
Vol 20 (1) ◽  
pp. 56-64
Author(s):  
Andhika Rakhmanda ◽  
Agung Pribadi ◽  
Parjiyo Parjiyo ◽  
Bobby Indra Gunawan Wibisono

This research aimed to evaluate the production performance of white shrimp Litopenaeus vannamei with super-intensive culture on different rearing densities. The research was conducted at PT. Dewi Laut Aquaculture, Cikelet, Garut, West Java. As many of 8 ponds were used and divided into 2 groups based on the stock density of shrimp, 550 ind/m2 and 650 ind/m2, and reared for 99 days. The results showed that super-intensive shrimp culture at the density of 550–650 ind/m2 potentially produced shrimp with average body weight ranged from 15.91–19.31 g, survival rate 62.67–87.95%, growth 0.16 to 0.20 g/day, FCR 1.35–1.66, and productivity reach 5.55–9.19 kg/m2. There were no significant differences between the two stocking densities in body weight, growth, and feed conversion performance, while ponds with higher rearing density had better survival and productivity than ponds with lower rearing density. L. vannamei cultured at a density of 650 ind/m2 produces the best performance and most feasible to be applied in super-intensive white shrimp cultivation. Keywords: Litopenaeus vannamei, super-intensive, high-density, production performance   ABSTRAK Penelitian ini bertujuan untuk mengevaluasi kinerja produksi udang vaname pada sistem super- intensif dengan padat penebaran berbeda. Penelitian dilaksanakan di tambak PT. Dewi Laut Aquaculture, Cikelet, Garut, Jawa Barat, menggunakan 8 petak tambak. Tambak dibagi menjadi 2 kelompok, masing-masing 4 petak tambak dengan padat tebar udang 550 ekor/m2 dan 4 petak tambak lainnya dengan padat tebar 650 ekor/m2 dengan masa pemeliharaan 99 hari. Hasil penelitian menunjukkan bahwa budidaya udang vaname pada sistem super-intensif dengan padat tebar 550–650 ekor/m2 dapat menghasilkan udang dengan bobot rata-rata berkisar antara 15.91–19.31 g, sintasan 62.67–87.95%, pertumbuhan 0.16–0.20 g/hari, konversi pakan (FCR) 1.35–1.66, dan produktivitas mencapai 5.55–9.19 kg/m2. Tidak ada perbedaan nyata antara kedua padat penebaran pada kinerja bobot, pertumbuhan harian, dan FCR; sementara tambak dengan kepadatan tinggi memiliki nilai sintasan dan produktivitas yang lebih tinggi dari tambak dengan kepadatan rendah. Padat penebaran 650 ekor/m2 menghasilkan kinerja produksi terbaik dan paling layak untuk diaplikasikan dalam budidaya udang vaname super-intensif. Kata kunci : Litopenaeus vannamei, padat tebar tinggi, super-intensif, kinerja produksi


2020 ◽  
Vol 21 (15) ◽  
pp. 5444
Author(s):  
Xiaoxi Zhang ◽  
Jianbo Yuan ◽  
Xiaojun Zhang ◽  
Jianhai Xiang ◽  
Fuhua Li

The sesquiterpenoid methyl farnesoate (MF), a juvenile hormone (JH) analog, plays important roles in many physiological processes of crustaceans, such as morphogenesis, molting and reproduction. Juvenile hormone esterase-like (JHE-like) carboxylesterase (CXE) is a key enzyme in MF degradation, playing a significant role in regulating MF titer. However, its function is barely known in shrimp. In this study, a total of 21 JHE-like CXEs (LvCXEs) were characterized in Pacific white shrimp Litopenaeus vannamei, based on the full genome and multi-transcriptomic data. LvCXE has a conserved triplet catalytic site (Ser-Glu-His) and a characteristic GxSxG motif. Most LvCXEs were highly expressed in the hepatopancreas, which was the main site for MF degradation. LvCXEs containing a GESAG motif showed a specific expansion in the L. vannamei genome. Those GESAG-containing LvCXEs presented differential expressions at different larvae stages and different molting stages of L. vannamei, which suggested their potential functions in development and molting. Additionally, when the transcription level of CXEs was inhibited, it could lead to failed molt and death of L. vannamei. When we further detected the expression levels of the key ecdysone responsive transcription factors including LvE75, LvBr-C, LvHr3 and LvFtz-f1 after the CXE inhibitor was injected into L. vannamei, they all showed apparent down-regulation. These results suggested that the expansion of LvCXEs in the L. vannamei genome should contribute to the regulation of metamorphosis at larvae stages and frequent molting during the growth of L. vannamei.


Author(s):  
Sartika Tangguda ◽  
I Nyoman Dodik Prasetia

Limbah cair tambak udang vaname (L. vannamei) merupakan hasil buangan dari proses budidaya yang umumnya langsung dibuang ke perairan umum. Limbah tersebut masih mengandung sejumlah unsur hara yang diperlukan untuk pertumbuhan organisme pada tingkatan trofik dibawahnya, salah satunya adalah mikroalga Chlorella sp. Namun, pada limbah tersebut masih ditemukan sejumlah organisme yang mengganggu pertumbuhan Chlorella sp. sehingga limbah harus disterilkan terlebih dahulu sebelum digunakan untuk menumbuhkan mikroalga tersebut. Tujuan dari penelitian ini adalah untuk mengetahui dosis limbah cair tambak udang vaname steril dalam produksi Chlorella sp. Penelitian ini menggunakan metode eksperimental dengan 4 perlakuan, yaitu A (dosis limbah 25%), B (dosis limbah 50%), C (dosis limbah 75%), dan D (dosis limbah 100%); masing-masing perlakuan diulang sebanyak 3 kali. Hasil penelitian menyatakan bahwa perlakuan D memberikan produksi Chlorella sp. tertinggi yang dapat dilihat dari kepadatan sel dan laju pertumbuhan spesifik pada saat puncak pertumbuhan, yaitu 2.833.333 sel/mL dan 0,472. Dari hasil penelitian ini dapat disimpulkan bahwa limbah cair tambak udang vaname steril dengan dosis 100% dapat digunakan untuk mengkultur Chlorella sp. skala laboratorium dengan menghasilkan produksi mikroalga tertinggi. Diharapkan limbah yang berasal dari hasil budidaya udang vaname dapat dimanfaatkan untuk mengkultur mikroalga sehingga mengurangi pencemaran yang terjadi di perairan umum sekitarnya. Liquid waste of vaname shrimp ponds (L. vannamei) is the result of waste from the cultivation process which is generally discharged directly into public waters. The waste still contains a number of nutrients needed for the growth of organisms at the lower trophic level, one of which is Chlorella sp. However, in these wastes there are still organisms that interfere with the growth of Chlorella sp. therefore the waste must be sterilized before using it to grow the microalgae. The purpose of this study was to determine the dose of liquid waste of sterile vaname shrimp ponds in the production of Chlorella sp. This study used an experimental method with 4 treatments, namely A (25% waste dose), B (50% waste dose), C (75% waste dose), and D (100% waste dose); each treatment was repeated 3 times. The results of the study stated that treatment D gave the production of Chlorella sp. the highest can be seen from cell density and specific growth rate at the peak of growth, which is 2,833,333 cells / mL and 0.472. The results of this study can be concluded that sterile liquid waste of vaname shrimp ponds with a dose of 100% can be used to culture Chlorella sp. laboratory scale by producing the highest microalgae production. It is expected that waste derived from vaname shrimp culture can be used to cultivate microalgae so as to reduce pollution in the surrounding public waters


2019 ◽  
Vol 5 (1) ◽  
pp. 49
Author(s):  
Andre Rekasana, Laksmi Sulmartiwi, Soedarno

Abstract White shrimp vannamei (Litopenaeus vannamei) is the main commodities among shrimp farmers because it has a rapid growth, can be cultivated with high density and world market demand is large enough .Since being introduced in Indonesia, vannamei shrimp has spread to the north of East Java pesisisr like Probolinggo, Pasuruan, Lamongan and Tuban. Shrimp growth factors are must be faced on vannamei shrimp culture, including the problems of disease. One type of virus disease is an Infectious Myo Necrosis Virus (IMNV). IMNV has affecting to the 70% of shrimp mortality (Tang et al., 2008). This study aims to determine the distribution of IMNV on white shrimp vannamei from the northern coast of East Java. This research uses descriptive method by taking samples from five different location which 75 samples totaly. In this research also take the water quality parameter including pH, DO, salinity and temperature. Whole of the samples performed the PCR test to determine how many samples which is indicating of IMNV. Based on measurements of water quality at five different locations, the plots I, II and III obtained the temperature range between 29.3-30.2 °C, salinity 90-30 ppt, pH 7.5-7.9, and DO 3.8-5.7 ppm. Temperature, pH and DO parameters show normally on white shrimp vannamei, while salinity shows different valve from normal conditions. Abnormallity happened in Situbondo with the salinity betwen 32-33 ppt and Pasuruan betwen 9-10 ppt. Results showed that the number of the lowest infection is Lamongan (53%) following Probolinggo (60%), Tuban (67%), Pasuruan (73%) and the highest is from Situbondo (100%)


2018 ◽  
Vol 6 (2) ◽  
pp. 691
Author(s):  
Luqman Hakim ◽  
Supono Supono ◽  
Yudha Trinoegraha Adiputra ◽  
Sri Waluyo

Pacific white shrimp (Litopenaeus vannamei) is new species were culture to replace tiger shrimp (Penaeus monodon) in Purworejo village, Pasir Sakti sub-district of East Lampung residence. This study was purposed to investigate culture performances of Pacific white shrimp in semi intensive system in two farmer groups. Eight ponds with ± 2100 m2/ ponds and density of 60 ind/m2 was used for this study. Results showed that Pacific white shrimp performances was different among two farmer groups. Sido Makmur group showed better performance compared to Lestari Gemilang with showed of ponds productivity 1337,8 kg and 1330,3 kg, respectively. FCR and SR among two groups also showed different, there were 1,88 to 2,39 and 86,95% to 63,3%, respectively. High mortality after WSSV infection was decreased pond productivity. Water quality parameters such as ammonia (0,029-0,031 ppm) and light density (<40 cm) were not suitable for shrimp culture impacted to pond productivity. WSSV infection and water quality parameters had corellation to pond performances.


2008 ◽  
Vol 3 (2) ◽  
pp. 139 ◽  
Author(s):  
Taukhid Taukhid ◽  
Yani Lestari Nur’aini

The aquaculture industry in Indonesia has been growing rapidly and plays an important role in rural development and export earning. Penaeid shrimp culture in Indonesia has become a leading export earning in fisheries sector. The main constraint encountered with shrimp culture has always been associated with disease outbreaks, especially, caused by viral agents. The Pacific white shrimp (Litopenaeus vannamei) was unofficially introduced to Indonesia in 1999, and officially approved by Indonesian government in 2001. By the end of 2007, the Pacific white shrimp has been cultured in more than 17 provinces. The Taura Syndrome (TS) disease was detected in Indonesia in 2002, and the disease is currently found in at least 10 provinces. The Infectious Myonecrosis (IMN) is an emerging disease for L. vannamei in Indonesia, first detected in May-June 2006, causing significant mortalities in grow-out ponds. The IMN is characterized by an acute onset of gross signs: focal to extensive whitish necrotic areas in the striated muscle, especially on the distal abdominal segments and tail fan. White necrotic areas become reddened similar to the color of cooked shrimp. The outbreak resulted in elevated mortalities was initially associated with a chronic course of persistent low level mortalities. Up to date, IMN was detected in East Java, Bali, and West Nusa Tenggara provinces. This paper is a brief review of the epidemiological study of IMN disease of Pacific white shrimp in Indonesia: the status of outbreaks, surveillance, and disease diagnosis, and control measures.


2008 ◽  
Vol 3 (1) ◽  
pp. 59 ◽  
Author(s):  
Taukhid Taukhid ◽  
Hambali Supriyadi ◽  
Isti Koesharyani

Penaeid shrimp culture is a major contributor to foreign exchange earning in Indonesia. It has significant impact on economic development of fisheries sector, and leads to be one of prime mover to improve social prosperity. However, shrimp industry particularly black tiger shrimp (Penaeus monodon) has been facing unpredictable situation due to disease problem. The main constrain in correlation to the development of shrimp industry is disease outbreak, especially caused by viral agents. White Spot Syndrome Virus (WSSV) occurred in 1994, causing mass mortality of black tiger shrimp almost in all of the middle and western part of Indonesia. Due to the disease problem, it is estimated that in year 2000, more than 50% of shrimp pond were idle. Pacific white shrimp (Litopenaeus vannamei) or “udang vanamei” was introduced to Indonesia at the end of 1999, and released officially in July, 2001. Response of shrimp farmers to the shrimp rapidly accepted and distributed to many provinces in the country. At the end of 2006, distribution of white shrimp culture was encountered in more than 15 provinces. The seeds are mainly produced from hatcheries located in East Java and Lampung. The information of TSV in Indonesia was reported firstly from East Java at the end of 2002, without a clear history. Since then, survey of TSV distribution was conducted intensively in white shrimp production areas. Beside TSV, population of white shrimp coming to Indonesia also susceptible to White Spot Syndrome Virus (WSSV) and Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) infection. A survey with the aim to know significant viral diseases of white shrimp is needed to set up an alternative strategy to control them. The survey was conducted, firstly in the main production centers of white shrimp; and planned to be continued throughout the country. Samples collection, diagnostic method and data compiled in this study were collected from both active and passive surveillance. Diagnosis of viral diseases infecting white shrimp in this study was focused on TSV, WSSV, and IHHNV agents. Polymerase Chain Reaction (PCR) test has been used as a major diagnostic technique in this study. Progress report of the study showed that TSV spreading limited in controlled areas. The study proved that WSSV and IHHNV have been found in cultured white shrimp. All of the diseases mentioned above tend to be a significant constrain of future white shrimp industry in Indonesia, and special attention should be given in order to protect wide-spread of particular disease from infected to uninfected ones. Also, briefly current status of white shrimp culture development in the country will be discussed in this paper.


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