scholarly journals Protective Effect of Ipomoea batatas L Leaves Extract on Histology of Pancreatic Langerhans Islet and Beta Cell Insulin Expression of Rats Induced by Streptozotocin

Molekul ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 48
Author(s):  
Dody Novrial ◽  
Soebowo Soebowo ◽  
Parno Widjojo
Keyword(s):  
Blood Glucose ◽  
Protective Effect ◽  
Ipomoea Batatas ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Sprague Dawley ◽  
Control Groups ◽  
Protection Effect ◽  
Treatment Groups ◽  
Sprague Dawley Rats

Sweet potato (Ipomoea batatas L) leaf is one of well known vegetables among Indonesian people.  It is also often used as traditional medicine for diabetes mellitus.  This research aimed to investigate the protective effect of I. batatas L leaves extract on the pancreas of IDDM animal model.  Twenty five male Sprague-Dawley rats were divided into 3 treatment groups, and 2 control groups.  Streptozotocin (STZ) was injected at multiple low doses (40 mg/kg BW) intraperitoneally for 5 consecutive days,  I. batatas L leaves extract (doses 0.25, 0.8, and 2.5 g/ kg BW) were administered for 14 days after the first STZ injection.  Fasting blood glucose was analyzed after complete STZ induction (day 6), and after 14 days treatment.  At the end of the study, rats were terminated, and pancreas were removed for histological examination and immunohistochemical procedure using anti-insulin antibody.  Diabetic rats treated with 2.5 g/kg BW I. batatas L leaves extract showed lowest fasting blood glucose among treatment groups, and had approximately 50% normal Langerhans islets with functional beta cells.  These results suggest that I. batatas L leaves extract has anti diabetic activity through its protection effect on the pancreas.

scholarly journals Azadirachta excelsa Improves Renal Morphology and Function in Streptozotocin Induced-Diabetic Sprague Dawley Rats

2020 ◽  
Vol 17 (12) ◽  
pp. 1307-1320
Author(s):  
Nur Syimal Aain AZMI ◽  
Nooraain HASHIM ◽  
Nurdiana SAMSULRIZAL ◽  
Noor Syaffinaz NOOR ◽  
Mohamad ZIN
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Structure And Function ◽  
Therapeutic Effects ◽  
Sprague Dawley ◽  
Standard Drug ◽  
Sprague Dawley Rats ◽  
Renal Morphology ◽  
And Function

Long term diabetes mellitus (DM) is associated with serious complications such as nephropathy. Previous studies revealed the ability of A. excelsa leaf extract treatment to reduce fasting blood glucose (FBG) in streptozotocin (STZ)-induced diabetic rats. The aim of this study was to determine the effect of A. excelsa extract in delaying the progression of diabetic nephropathy by evaluating the kidney structure and function. The effects were compared with 2 positive controls, which were metformin (standard drug) and quercetin (plant active compound). Induction of diabetic conditions was conducted by the intraperitoneal (IP) injection of STZ (60 mg/kg bwt) in male Sprague Dawley rats. The experimental animals were grouped into: 1) normal control (NC, saline); 2) diabetic control (DC, saline); 3) metformin-treated diabetic rats (DMET, 1000 mg/kg bwt); 4) quercetin-treated diabetic rats (DQ, 40 mg/kg bwt), and 5) A. excelsa-treated diabetic rats (DAE, 250 mg/kg bwt). All treatments were given once daily for 8 weeks through oral gavage. The inter-relation between the changes in the fasting blood glucose and kidney oxidative stress, structure, and function was evaluated. The results showed a significant increase (p < 0.05) of MDA and SOD level and a decrease (p < 0.05) of GPx levels, plus distortion of renal morphology among the DC and DMET groups. Meanwhile, both DQ and DAE groups showed significant reduction (p < 0.05) of MDA levels and elevation (p < 0.05) of SOD and GPx levels. The quercetin and A. excelsa treatments also improved the kidney function parameters and morphological changes of the diabetic rats. These findings indicate that quercetin and A. excelsa possess renal therapeutic effects.

KELULUT HONEY SUPPLEMENTATION PREVENTS SPERM AND TESTICULAR OXIDATIVE DAMAGE IN STREPTOZOTOCIN-INDUCED DIABETIC RATS

2017 ◽  
Vol 79 (3) ◽  
Author(s):  
Siti Balkis Budin ◽  
Fatin Farhana Jubaidi ◽  
Siti Nur Farahana Mohd Noor Azam ◽  
Nur Liyana Mohamed Yusof ◽  
Izatus Shima Taib ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Sperm Quality ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Sprague Dawley ◽  
Control Groups ◽  
Sod Activity ◽  
Male Adult ◽  
Pathological Conditions ◽  
Sprague Dawley Rats

Previous studies found that Kelulut Honey produced by Trigona spp. bees is able to prevent oxidative damage in various pathological conditions.  Thus, the present study aimed to determine whether Kelulut Honey could prevent the sperm and testicular damage in streptozotocin-induced diabetic rats. Male Adult male Sprague-Dawley rats were divided into four groups: Non-Diabetic (NDM), Non-Diabetic with Kelulut Honey supplementation (NDMKH), Diabetic without supplementation (DM) and Diabetic with Kelulut Honey supplementation (DMKH).  Kelulut honey was given at the dose of 2.0 g/kg weight daily via gavage for 28 consecutive days. Results showed that sperm quality produced by diabetic rats supplemented with Kelulut honey significantly improved compared to the diabetic control groups (p<0.05). SOD activity and GSH level increased significantly (p<0.05) whereas PC and MDA levels significantly decreased in sperm and testis of DMKH rats when compared to DM rats (p<0.05). Histological observation showed obvious increase in spermatozoa in the lumen of epididymis and increased spermatogenic cells density in the testis of DMKH group.  In conclusion, Kelulut Honey has a potential in preventing the damage of sperm and testis in diabetic rats.

scholarly journals Identification of Potential Therapeutic Targets in the Liver of Pioglitazone-Treated Type 2 Diabetes Sprague-Dawley Rats via Expression Profile Chip and iTRAQ Assay

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Zhong-Xia Lu ◽  
Wen-Jun Xu ◽  
Yang-Sheng Wu ◽  
Chang-Yu Li ◽  
Yi-Tao Chen
Keyword(s):  
Type 2 Diabetes ◽  
Blood Glucose ◽  
Differentially Expressed Genes ◽  
Fasting Blood Glucose ◽  
Treated Group ◽  
Differentially Expressed ◽  
Model Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

The aim of the present study was to identify key antidiabetic nodes in the livers of pioglitazone-treated type 2 diabetes mellitus Sprague-Dawley rats by transcriptomic and proteomic analysis. Rats were randomly divided into the control, the diabetes model, and the pioglitazone-treated groups. After treatment with pioglitazone for 11 weeks, the effects on fasting blood glucose, body weight, and blood biochemistry parameters were evaluated. Microarray and iTRAQ analysis were used to determine the differentially expressed genes/proteins in rat livers. 1.5-fold changes in gene expression and 1.2-fold changes in protein were set as the screening criteria. After treatment with pioglitazone for 11 weeks, fasting blood glucose in pioglitazone-treated rats was significantly lower than that in the model group. There was a tendency for pioglitazone to reduce TC, TG, TP, ALB, BUN, and HDL-c levels. Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) were applied to analyze differentially expressed genes/proteins. Furthermore, Western blotting and RT-qPCR were used to validate the results of microarray and iTRAQ. In conclusion, Cyp7a1, Cp, and RT1-EC2 are differentially expressed genes/proteins since they showed a similar trend in rats in the model group and the pioglitazone-treated group.

scholarly journals Antecedent glycemic control reduces severe hypoglycemia-induced neuronal damage in diabetic rats

2013 ◽  
Vol 304 (12) ◽  
pp. E1331-E1337 ◽  
Author(s):  
Candace M. Reno ◽  
Tariq Tanoli ◽  
Adam Bree ◽  
Dorit Daphna-Iken ◽  
Chen Cui ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Brain Damage ◽  
Insulin Treatment ◽  
Neuronal Damage ◽  
Blood Glucose Control ◽  
Severe Hypoglycemia ◽  
Diabetic Rats ◽  
Sprague Dawley ◽  
Glucose Levels ◽  
Sprague Dawley Rats

Brain damage due to severe hypoglycemia occurs in insulin-treated people with diabetes. This study tests the hypothesis that chronic insulin therapy that normalizes elevated blood glucose in diabetic rats would be neuroprotective against brain damage induced by an acute episode of severe hypoglycemia. Male Sprague-Dawley rats were split into three groups: 1) control, non-diabetic; 2) STZ-diabetic; and 3) insulin-treated STZ-diabetic. After 3 wk of chronic treatment, unrestrained awake rats underwent acute hyperinsulinemic severe hypoglycemic (10–15 mg/dl) clamps for 1 h. Rats were subsequently analyzed for brain damage and cognitive function. Severe hypoglycemia induced 15-fold more neuronal damage in STZ-diabetic rats compared with nondiabetic rats. Chronic insulin treatment of diabetic rats, which nearly normalized glucose levels, markedly reduced neuronal damage induced by severe hypoglycemia. Fortunately, no cognitive defects associated with the hypoglycemia-induced brain damage were observed in any group. In conclusion, antecedent blood glucose control represents a major modifiable therapeutic intervention that can afford diabetic subjects neuroprotection against severe hypoglycemia-induced brain damage.

scholarly journals Myrmecodia platytyrea Methanol Tuber Extract Ameliorates Hyperglycemia In STZ-Induced Diabetic Sprague-Dawley Male Rats

2021 ◽  
pp. 338-348
Author(s):  
Mizaton Hazizul Hasan ◽  
Hasbullani Zakaria ◽  
Ibtisam Abdul Wahab ◽  
Thellie Ponto ◽  
Aishah Adam
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Diabetic Rats ◽  
Male Rats ◽  
Current Therapy ◽  
Diabetic Patients ◽  
Sprague Dawley ◽  
Tuber Extract

Type 2 diabetes mellitus (T2DM) is one of the main non-communicable chronic diseases that has many complications that compromise the quality of life. Hence, the need to find alternatives to replace the current therapy or as an adjuvant. Tubers of Myrmecodia platytytrea (Rubiaceae) has been used traditionally as an alternative therapy for the management of cancer and other inflammatory-related disorders. The aim of this study was to investigate the potency of M. platytytrea methanolic tuber extract (MPMTE) as an antihyperglycemic agent, in vivo. :The streptozotocin (STZ)-induced diabetic rats were treated orally with MPMTE (100, 200 and 400 mg/kg) and metformin (positive control, 100 mg/kg) daily for 14 days. Blood glucose level and other biochemistry analysis were conducted including histological examination on liver, kidney and pancreas.  The STZ-induced diabetic rats treated with MPMTE (200 and 400 mg/kg) had significant decreased (p<0.05) in fasting blood glucose, total cholesterol, triglycerides and low-density lipoprotein (LDL) with no significant changes in high-density lipoprotein (HDL) compared to STZ-induced untreated diabetic rats. Liver, kidney and pancreas were devoid of any damage caused by STZ.  MPMTE had strong antihyperglycaemic activity and was protective against any STZ-induced organ damage. Thus, MPMTE can be further developed into an adjuvant therapy for diabetic patients.

Obesity-Induced Hypertension Develops in Young Rats Independently of the Renin-Angiotensin-Aldosterone System

2006 ◽  
Vol 231 (3) ◽  
pp. 282-287 ◽  
Author(s):  
Anita D. Smith ◽  
Michael W. Brands ◽  
Mong-Heng Wang ◽  
Anne M. Dorrance
Keyword(s):  
Blood Pressure ◽  
Blood Glucose ◽  
Vascular Reactivity ◽  
Fasting Blood Glucose ◽  
Plasma Renin ◽  
Diet Group ◽  
Sprague Dawley ◽  
Diet Induced Obesity ◽  
Sprague Dawley Rats ◽  
Induced Hypertension

A correlation exists between obesity and hypertension. In the currently available models of diet-induced obesity, the treatment of rats with a high fat (HF) diet does not begin until adulthood. Our aim was to develop and characterize a model of pre-pubescent obesity-induced hypertension. Male Sprague-Dawley rats were fed a HF diet (35% fat) for 10 weeks, beginning at age 3 weeks. Blood pressure was measured by tail-cuff, and a terminal blood sample was obtained to measure fasting blood glucose, insulin, plasma renin, aldosterone, thiobarbitutic acid reactive substances (TBARS), and free 8-isoprostanes levels. The vascular reactivity in the aorta was assessed using a myograph. Blood pressure was increased in rats fed the HF diet (HF, 161 ± 2 mm Hg vs. control, 137 ± 2 mm Hg, P < 0.05). Blood glucose (HF, 155 ± 4 mg/dL vs. control, 123 ± 5 mg/dL, P < 0.05), insulin (HF, 232 ± 63 pM vs. control, 60 ± 11 pM, P < 0.05), TBARS (expressed as nM of malondialdehyde [MDA]/ml [HF, 1.8 ± 0.37 nM MDA/ml vs. control 1.05 ± 0.09 nM MDA/ml, P < 0.05]), and free 8-isoprostanes (HF, 229 ± 68 pg/ml vs. control, 112 ± 9 pg/ml, P < 0.05) levels were elevated in the HF diet group. Interestingly, plasma renin and aldosterone levels were not different between the groups. The maximum vasoconstriction to phenylephrine (10−4 M) was increased in the HF diet group (HF, 26.1 ± 1.5 mN vs. control 22.3 ± 1.2 mN, P < 0.05). In conclusion, pre-pubescent rats become hypertensive and have increased oxidative stress and enhanced vasoconstriction when fed a HF diet. Surprisingly, this occurs without the increase in renin or aldosterone levels seen in the adult models of diet-induced obesity.

scholarly journals Anti-hyperglycemic Effect of Torbangun (Coleus amboinicus Lour) Leaves Extract Through Liver and Muscle Glycogen Deposits in Streptozotocin-induced Hyperglycmic Sprague-Dawley Rats Model

2019 ◽  
Author(s):  
Meilla Dwi Andrestian ◽  
Rizal Damanik ◽  
Faisal Anwar ◽  
Nancy Dewi Yuliana
Keyword(s):  
Blood Glucose ◽  
Muscle Glycogen ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Liver Glycogen ◽  
Sprague Dawley ◽  
Β Cells ◽  
Insulin Levels ◽  
Glucose Levels ◽  
Sprague Dawley Rats

The association of liver and muscle glycogen deposits with serum insulin levels, β-cells pancreas, and fasting blood glucose (FBG) of streptozotocin (STZ)-induced hyperglycemic rats receiving Torbangun leaves extract (TE) investigated. The intervention performed on 25 8-week-old Sprague-Dawley rats divided into four groups. Seven rats separated as a normal group (N), and other rats injected with streptozotocin (STZ). Confirmation of hyperglycemic was characterized by fasting blood glucose >126 mg/dl. Treatment group which is NG (hyperglycemic rats); N (normal rats); H-IM (62.5 mg/kg BW metformin); and H-IT (620 mg/kg BW TE) for 14 days. This study revealed that TE significantly decreased FBG levels, increased insulin production, and the amount of liver glycogen deposits (a=0.01). However, the intervention did not significantly increase the amount of muscle glycogen deposits. TE administration improves β-cells, increases the liver and muscle glycogen deposits. TE was shown to have antihyperglycemic activity by improving the β-cell, increasing blood serum insulin levels, decreasing blood glucose levels, and increasing the liver glycogen deposits.

scholarly journals Pengaruh Pemberian Koro Benguk (Mucuna pruriens L) terhadap Kadar Gula Darah Tikus Diabetes Melitus yang Diinduksi Streptozotocin

2020 ◽  
Vol 2 (2) ◽  
pp. 136-140
Author(s):  
Endang Widhiyastuti ◽  
Mastuti Widi Lestari
Keyword(s):  
Blood Glucose ◽  
Blood Sugar ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Observation Time ◽  
The Body ◽  
Mucuna Pruriens ◽  
Control Group ◽  
Sprague Dawley ◽  
Sugar Levels

Diabetes which is well-known in the community as diabetes in Indonesia is a chronic disease, which occurs when the pancreas does not produce enough insulin or when the body cannot utilize the insulin produced by its own products. The Provision of antioxidants in DM mice can reduce blood sugar levels. One of the herbs that can be used for control and management of blood sugar in diabetes is swollen koro. Koro Benguk (Mucuna pruriens L) is a plant that can be used as an alternative treatment because it contains antioxidants that can maintain health without causing toxic effects. The purpose of this study was to determine whether there is an effect of giving koro benguk coffee (Mucuna pruriens L) on blood sugar levels of Streptozotocin-induced Diabetes Mellitus Rats. This study is an experimental study of Sprague Dawley mice. A total of 35 male wistar rats were divided into 5 groups each: normal control (K1); diabetes control (K2); diabetic rats were given a large coffee extract 0.63 mg / g BW rat (P1); diabetic rats were given a large infusion of coffee koro 1.26 mg / g BW rats (P2). Diabetic rats were given an infusion of coffee koro benguk20,52 mg / g BW rats. Fasting blood glucose (GDP) levels were analyzed weekly for 3 weeks using the GOD-PAP method. The results of the study showed a decrease in blood sugar for 4 times the observation time in almost all treatment groups except the positive control group. The conclusions in this study were the provision of related coffee (Mucuna pruriens L) can reduce fasting blood glucose levels in Sprague Dawley rats with diabetes models significantly compared to controls.

Insulin administration abrogates perturbation of methyl group and homocysteine metabolism in streptozotocin-treated type 1 diabetic rats

2011 ◽  
Vol 301 (3) ◽  
pp. E560-E565 ◽  
Author(s):  
Kristin M. Nieman ◽  
Kevin L. Schalinske
Keyword(s):  
Blood Glucose ◽  
Regulatory Protein ◽  
Diabetic Rats ◽  
Plasma Homocysteine ◽  
Future Research ◽  
Sprague Dawley ◽  
Methyl Group ◽  
Sprague Dawley Rats ◽  
Blood Glucose Concentrations

Modifications in methyl group and homocysteine metabolism are associated with a number of pathologies, including vascular disease, cancer, and neural tube defects. A diabetic state is known to alter both methyl group and homocysteine metabolism, and glycine N-methyltransferase (GNMT) is a major regulatory protein that controls the supply and utilization of methyl groups. We have shown previously that diabetes induces GNMT expression and reduces plasma homocysteine pools by stimulating both its catabolism and folate-independent remethylation. This study was conducted to determine whether insulin plays a role in the control of homocysteine concentrations and GNMT as well as other key regulatory proteins. Male Sprague-Dawley rats were randomly assigned to one of three groups: control, streptozotocin (STZ)-induced diabetic (60 mg/kg body wt), and insulin-treated diabetic (1.0 U bid). After 5 days, rats were anesthetized (ketamine-xylazine) for procurement of blood and tissues. A 1.5-fold elevation in hepatic GNMT activity and hypohomocysteinemia in diabetic rats was completely prevented by insulin treatment. Additionally, diabetes-mediated alterations in methionine synthase, phosphatidylethanolamine N-methyltransferase, and DNA methylation were also prevented by insulin. We hypothesize that the concentration of blood glucose may represent a regulatory signal to modify GNMT and homocysteine. In support of this, blood glucose concentrations were negatively correlated with total plasma homocysteine ( r = −0.75, P < 0.001) and positively correlated with GNMT activity ( r = 0.77, P < 0.001). Future research will focus on further elucidating the role of glucose or insulin as a signal for regulating homocysteine and methyl group metabolism.

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