Vorinostat Pretreatment Enhanced Ciprofloxacin-Induced Antibacterial Activity

The mechanism of ciprofloxacin action involves interference with transcription and replication of bacterial DNA, which results in elevated oxidative stress, and bacterial cell death. Vorinostat was shown to induce oxidative DNA damage. In the current work, the possibility for interactive effect of vorinotat on ciprofloxacin-induced cytotoxicity against a number of reference bacteria was investigated. Standard bacterial strains were Escherichia coli ATCC 35218, Staphylococcus aureus ATCC29213, Pseudomonas aeruginosa ATCC 9027, Staphylococcus epidermidis ATCC 12228, Acinetobacter baumannii ATCC 17978, Proteus mirabilis ATCC 12459, Klebsiella pneumoniae ATCC 13883, methicillin-resistant Staphylococcus aureus (MRSA) (ATCC 43300), and Streptococcus pneumoniae (ATCC 25923). The antibacterial activity of ciprofloxacin with or without pretreatment of bacterial cells by vorinostat was examined using disc diffusion procedure and determination of the minimum inhibitory concentration (MIC) and zones of inhibition of bacterial growth. All tested bacterial strains showed sensitivity to ciprofloxacin. When pretreated with vorinostat, significantly larger zones of inhibition and smaller MIC values were observed in all bacterial strains compared ciprofloxacin alone. As a conclusion, current results showed the possible agonistic properties for vorinostat when it is used together with ciprofloxacin. Future research will be focus on molecular mechanisms possible for such interactive effect.

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Antibacterial activity of seneciolactone isolated from Senecio scandens against some common gastrointestinal tract disease causing bacterial pathogens

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v11i1.24792 ◽

2016 ◽

Vol 11 (1) ◽

pp. 218

Author(s):

Yi Zheng ◽

Yu Wang ◽

Yuan-Hong Shang

Keyword(s):

Antibacterial Activity ◽

Gastrointestinal Tract ◽

Bacterial Pathogens ◽

Cellular Membrane ◽

Shigella Dysenteriae ◽

Cellular Morphology ◽

Bacterial Cells ◽

Bacterial Strains ◽

Zones Of Inhibition ◽

Tract Disease

The objective of the present investigation was to study the antibacterial effect of seneciolactone isolated from the methanolic extract of Senecio scandens against five bacterial pathogens which are known to cause several gastrointestinal tract diseases. Disc diffusion assay and agar well diffusion assays were used to examine the antibacterial efficacy of this compound by measuring zones of inhibition and MIC/MBC values. Scanning electron microscopy (SEM) was involved to study the effect of this compound on cellular morphology of Shigella dysenteriae. Results revealed that seneciolactone exhibited moderate to potent antibacterial activity against different bacterial strains. Zones of inhibition and MIC/MBC values indicated that seneciolactone was most potent against S. dysenteriae followed by Pseudomonas aeruginosa and E. coli. SEM results indicated that seneciolactone induced potent damage to the cell membrane of the tested bacteria. As compared to the untreated control which exhibited normal cellular morphology, the seneciolactone treated bacterial cells revealed severe damage to the cellular membrane particularly at the higher doses.

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Synthesis and Antibacterial Activity of New Spiro[thiadiazoline-(pyrazolo[3,4-d]pyrimidine)] Derivatives

Journal of Chemistry ◽

10.1155/2015/982404 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Mohammed El Fal ◽

Youssef Ramli ◽

Abdelfettah Zerzouf ◽

Ahmed Talbaoui ◽

Youssef Bakri ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Spectroscopic Data ◽

Heterocyclic Compounds ◽

Dipolar Cycloaddition ◽

Bacterial Strains ◽

Pyrimidine Derivatives ◽

H Nmr ◽

Biological Interest ◽

Mic Value

New heterocyclic compounds spiroderivatives of allopurinol of biological interest were prepared from allopurinol via thionation and 1,3-dipolar cycloaddition and were produced in high to excellent yields. These compounds were characterized on the basis of spectral and spectroscopic data (1H NMR,13C, IR, and MS). The antibacterial activity of the synthesized products was studied using bacterial strains:Staphylococcus aureus,Enterococcus faecalis,Escherichia coli, andPseudomonas aeruginosa. Compounds having an ethyl group showed the best activity with MIC value of 31.25 µg/mL againstStaphylococcus aureusandStreptococcus fasciens.

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FLORAL EXTRACT OF Tibouchina granulosa (DESR.) COGN. PHYTOCHEMISTRY PROSPECTION, AND ANTIBACTERIAL ACTIVITY

Ipê Agronomic Journal ◽

10.37951/2595-6906.2021v5i1.6880 ◽

2021 ◽

Vol 5 (1) ◽

pp. 1-9

Author(s):

Antonio Carlos Pereira de Menezes Filho ◽

Matheus Vinícius Abadia Ventura ◽

Carlos Frederico de Souza Castro

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Enterococcus Faecalis ◽

Floral Organ ◽

Salt Formation ◽

Bacterial Strains ◽

Positive Results

Tibouchina granulosa is a species that blooms annually in several regions of Brazil. This species is still little explored in terms of phytocompounds in all organs of this plant, especially the floral organ. Flowers of T. granulosa were collected in the municipality of Rio Verde, Goiás, Brazil, in 2021. The hydroethanolic floral extract was prepared by maceration and qualitative phytochemical (colorimetric reactions and salt formation) and antibacterial analyzes performed and the results expressed in millimeters of inhibition at different concentrations in mg mL-1. Several phytochemical classes were observed with positive results, especially for alkaloids, phenolics, oxylates, saponins, carbohydrates and tannins. As for the bacterial assay, potential antibacterial activity was observed for all bacterial strains tested, except for Salmonella serovar Thyphymurium and serovar Enteritidis. Expressive inhibitions were observed for Enterococcus faecalis > Pseudomonas aeruginosa > Staphylococcus aureus and Escherichia coli at the highest concentrations between 100-50 mg mL-1. The floral extract of Tibouchina granulosa showed phytotherapeutic potential with the presence of several phytochemical groups and expressive antibacterial activity.

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Synthesis and Antimicrobial Screening of Some Novel 5-Substituted-1,10b-dihydroimidazole[1,2-c]quinazoline Derivatives

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2021.14.2.6 ◽

2021 ◽

Vol 14 (2) ◽

pp. 5406-5411

Author(s):

Haribhai Rabari ◽

Hetal Vankar ◽

Beenkumar Prajapati

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Candida Albicans ◽

Antifungal Activity ◽

Aspergillus Niger ◽

Antifungal Compound ◽

Bacterial Strains ◽

Standard Drug ◽

Quinazoline Derivatives

The emergence of multidrug microbial resistance is the main challenges that the modern scientists have so far been facing in the recent era. In this respect, new series of drug classes having potential to give antimicrobial effect have been synthesized. A new series of 5- substituted-1,10 b-dihydroimidazole[1,2-c]quinazoline derivatives 8a-e have been synthesized and screened for antibacterial activity and antifungal activity. Synthesized derivatives were characterized by IR, MASS and 1H-NMR spectroscopy. Synthesized compounds show good activity, which was comparable to the standard drug and it can be useful for the further clinical study. Antibacterial activity was evaluated against four different pathogenic bacterial strains like Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudo-monas aeruginosa. Among the screened compounds, 8e show good antibacterial activity against Staphylococcus aureus and Escherichia coli with MIC of 50 and 100 μg/ml respectively. Antifungal activity was evaluated against two strains of fungi. Among the synthesized derivates, compound 8c was emerged out as the potent antifungal compound against Candida albicans and Aspergillus niger with MIC of 25 μg/ml and 75μg/ml respectively. Compound 8e also shows good antifungal activity with MIC of 50 μg/ml against both Candida albicans and Aspergillus niger. The overall results of this study indicated that synthesized quinazoline derivatives had the potential to act as an antibacterial and antifungal agent, hence further investigation is warranted.

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Utilization of Mn-Doped ZnSe/ZnS Core/Shell Quantum Dots for Rapid Detection of Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus

Advances in Materials Science and Engineering ◽

10.1155/2020/9718706 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Thi-Diem Bui ◽

Quang-Liem Nguyen ◽

Thi-Bich Luong ◽

Van Thuan Le ◽

Van-Dat Doan

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Quantum Dots ◽

Fluorescent Labeling ◽

Core Shell ◽

Bacterial Cells ◽

Bacterial Strains ◽

Methicillin Resistant ◽

E Coli ◽

Mn Doped

In this study, Mn-doped ZnSe/ZnS core/shell quantum dots (CSQDs) were synthesized in aqueous solution using polyethylene glycol as a surface stabilizer and successfully applied in the detection of Escherichia coli O157:H7 and methicillin-resistant Staphylococcus aureus (MRSA) for the first time. The CSQDs were conjugated with anti-E. coli antibody and anti-MRSA antibody via protein A supported by 1-ethyl-3-(-3-dimethylaminopropyl)carbodiimide hydrochloride for fluorescent labeling of the intact bacterial cells. The detection was performed for the bacterial strains cultivated in Luria-Bertani liquid medium. The obtained results indicate that E. coli O157:H7 and MRSA can be detected within 30 min at a high sensitivity of 101 CFU/mL. This labeling method based on the highly fluorescent CSQDs may have great potential for use in the food industry to check and prevent outbreaks of foodborne illness.

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Polysaccharide-Based Film of Cassia fistula and it's Antibacterial Activity

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.875-877.87 ◽

2014 ◽

Vol 875-877 ◽

pp. 87-90 ◽

Cited By ~ 1

Author(s):

Chutimon Satirapipathkul ◽

Tanakan Chatdum

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Antibacterial Properties ◽

Water Vapor Permeability ◽

Inhibition Zone ◽

Vapor Permeability ◽

Glycerol Concentration ◽

Bacterial Strains ◽

Cassia Fistula

The film-forming potential of isolate of seed polysaccharide fromCassia fistulawas investigated. Increasing the glycerol concentration in the film increased elongation at break, film solubility and water vapor permeability but decreased tensile strength (TS). The film impregnated with the acetone extract ofAtractylodes lanceawas assessed for inhibition ofEscherichia coliandStaphylococcus aureus. The obtained results showed that the films exhibited antibacterial activity against both bacterial strains. Disc-diffusion assay revealed that the film resulted in a larger inhibition zone around the film onStaphylococcus aureusthan it did onEscherichia coliat the same extract concentrations (0.1 to 0.9 wt %). It can be seen that the film has satisfactory physical and antibacterial properties.

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Antibacterial Diterpenes from the Roots of Ceriops tagal

Natural Product Communications ◽

10.1177/1934578x0800300104 ◽

2008 ◽

Vol 3 (1) ◽

pp. 1934578X0800300 ◽

Cited By ~ 1

Author(s):

Musa Chacha ◽

Renameditswe Mapitse ◽

Anthony J. Afolayan ◽

Runner R. T. Majinda

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacillus Cereus ◽

Spectroscopic Data ◽

Dilution Method ◽

Agar Dilution Method ◽

Bacterial Strains ◽

Agar Dilution ◽

Ceriops Tagal

Investigation of the roots of Ceriops tagal led to the isolation of a new isopimarane, together with the known diterpenes isopimar-8(14)-en-15,16-diol and erythroxyl-4(17),15(16)-dien-3-one. The structure of the new compound was identified as isopimar-8(14)-en-16-hydroxy-15-one. These structures were determined from extensive spectroscopic data analysis. The isolates were screened for antibacterial activity using the agar dilution method against ten test bacterial strains ( Bacillus cereus, Escherichia coli, Klebsiella pneumoniae, Micrococcus kristinae, Pseudomonas aeruginosa, Salmonella pooni, Serratia marcescens, Staphylococcus aureus, S. epidermidis and Streptococcus pyrogens). Isopimar-8(14)-en-16-hydroxy-15-one exhibited activity, with MIC values of 0.5 mg/mL against Streptococcus pyrogens; 0.25 mg/mL against Salmonella pooni and 0.1 mg/mL against Bacillus cereus, Staphylococcus aureus and Micrococcus kristinae.

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Photon-Induced Superior Antibacterial Activity of Palladium-Decorated, Magnetically Separable Fe3O4/Pd/mpg-C3N4 Nanocomposites

Molecules ◽

10.3390/molecules24213888 ◽

2019 ◽

Vol 24 (21) ◽

pp. 3888 ◽

Cited By ~ 1

Author(s):

Deepika Thakur ◽

Qui Thanh Hoai Ta ◽

Jin-Seo Noh

Keyword(s):

Antibacterial Activity ◽

Solution Method ◽

Pd Nanoparticles ◽

Bacterial Degradation ◽

Photoluminescence Intensity ◽

Bacterial Cells ◽

Bacterial Strains ◽

E Coli ◽

Carrier Recombination ◽

Solar Light Irradiation

Three-component nanocomposites (Fe3O4/Pd/mpg-C3N4) have been systematically synthesized using a three-step solution method for the photocatalytic bacterial decontamination. The mesoporous g-C3N4 nanosheets (mpg-C3N4), which were prepared by the acid treatment, showed a great improvement in photocatalytic performance. The photoluminescence intensity of the mpg-C3N4 nanosheets was disclosed to drop about 60% from the value of normal g-C3N4 nanosheets. Decoration of mpg-C3N4 with palladium (Pd) nanoparticles led to the effective suppression of carrier recombination and the carrier migration to Fe3O4 nanoparticles. It was revealed that the three-component nanocomposites degraded 99.9% of E. coli and 99.8% of S. aureus bacterial strains within 2 h of solar light irradiation at a 100 μg/mL concentration, demonstrating their superb photocatalytic antibacterial activity. In addition, the nanocomposites could be easily separated from the bacterial cells and repeatedly used for photocatalytic bacterial degradation with good recyclability. The strong photon-induced antibacterial activity and good recyclability of the three-component nanocomposites may represent their potential as a promising antibacterial photocatalyst.

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ANTIBACTERIAL ACTIVITY OF SOAP MADE FROM ESSENTIAL OILS AND COMMERCIAL SOAP SOLD IN THE LEBANESE MARKET

Bacterial Empire ◽

10.36547/be.304 ◽

2021 ◽

pp. e304

Author(s):

Candy GERGES ◽

Mohamad HADLA ◽

Michele ELIALI ◽

Martine RICHA ◽

Nour Mammari ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Antibacterial Activities ◽

Diffusion Method ◽

Bacterial Strains ◽

Effective Measure ◽

E Coli ◽

Different Types ◽

Commercial Soap ◽

Zones Of Inhibition

Background: Thousands of people are dying as a result of infections caused by bacteria. Among the main routes of germ transmission are the hands, making hand hygiene very important in preventing the spread of pathogens and bacterial infection. Hand washing with soap and water is considered to be a simple and effective measure. Old soap manufacturers have long had traditional uses in the Lebanese community. Methods: The purpose of this study is to compare the antibacterial activity of oil-based soaps with commercial soap sold in the Lebanese market. Different types of herbal soap and antiseptics have been used in this study. Four bacterial strains were used: Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli and Enterobacter Spp. The antibacterial activities of these soaps were determined by the diffusion method of disks in agar medium. Results and Discussion: Statistical analysis of zones of inhibition showed that S. epidermidis, P. aeruginosa and Enterobacter Spp. were sensitive only to traditional oil-based soaps, which are Sage, Rose Mary and Cedar. In addition, S. aureus showed sensitivity to soaps comprised of essential oils as well as antibacterial synthetic soap, Dettol and Lifebuoy. On the other hand, E. coli showed resistance to all soaps. Soaps comprised of natural essential oils have shown antibacterial activity superior to so-called “Antibacterial” soaps. Conclusion: Based on this study, we can say that the use of soaps with essential oils might be the best option due to their organic origin as well as their antibacterial proved activities.

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Escherichia coli and Staphylococcus aureus responsible for bovine mastitis sensitivity to the essential oil of Algerian Thymus fontanesii Boiss. et Reut.

Acta Scientifica Naturalis ◽

10.2478/asn-2020-0004 ◽

2020 ◽

Vol 7 (1) ◽

pp. 26-32

Author(s):

Bendella Amina nor elhouda ◽

Ghazi Kheira ◽

Meliani Samia

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Essential Oil ◽

Bovine Mastitis ◽

Diffusion Method ◽

Bacterial Strains ◽

E Coli ◽

Gram Positive Bacteria ◽

Zones Of Inhibition

AbstractThe aim of this study is to test two different methods for evaluating the in vitro antibacterial effect of Thymus fontanesii Boiss. et Reut. essential oil against standard and clinical bacterial strains responsible for bovine mastitis: the disc diffusion method or the aromatogram which allows the demonstration of the antibacterial power of essential oils on the bacterial strains tested, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and two strains isolated from bovine mastitis milk S. aureus and E. coli. The inhibition activity of the essential oil of T. fontanesii on bacterial strains by the two methods shows that the antimicrobial power of this oil is very important and is characterized by bactericidal and bacteriostatic action against gram negative and gram positive bacteria. The antimicrobial evaluation by the aromatogram showed good antibacterial activity against all the strains tested, the zones of inhibition of the bacteria were between 23,33±1,527mm and 37,5±3,535 mm. The search for minimum inhibitory concentrations MIC and bactericides CMB made it possible to quantitatively assess the antimicrobial power of this essential oil. In this work, the MIC was 0,625 µl/ml for all strains tested, and the lowest CMB was that of T. fontanesii against E. coli ATCC 25922 was 0,625 µl/ml.

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