scholarly journals Application of Saccharomyces cerevisiae as a Probiotic for Producing Low Cholesterol and Antibiotic-Free Broiler Meat

2020 ◽  
Vol 44 (2) ◽  
Author(s):  
Suci Wulandari ◽  
Theo Mahiseta Syahniar ◽  
Dadik Pantaya
Keyword(s):  
Intestinal Tract ◽  
Cholesterol Content ◽  
Abdominal Fat ◽  
Health Improvement ◽  
Breast Meat ◽  
Broiler Meat ◽  
Treatment Groups ◽  
E Coli ◽  
Commercial Feed ◽  
Low Cholesterol

The aim of this study was to evaluate the application of S. cerevisiae as a probiotic for producing low cholesterol and antibiotic-free broiler meat. This study used 250 broilers (14 days old) which were divided into 5 treatment groups, those were T0 (rations without S. cerevisiae addition), T1, T2 and T3 (rations added with 0.5, 1, and 1.5 g/kg DM of S. cerevisiae respectively), and K group (commercial feed). Broilers were reared until 36 days old prior to slaughter. The carcass quality, abdominal fat, internal organs, cholesterol content of breast meat, the number of LAB and E. coli of intestinal tract were observed. The addition of S. cerevisiae tended to increase the number of LAB in intestinal tract, whereas the number of E. coli significantly decreased. The highest slaughter weight was found in group fed with commercial feed. There were no significant effects of S. cerevisiae addition on carcass, abdominal fat and weight of internal and immunity organs. The lowest cholesterol content was showed on broiler fed with rations added with 1 g/kg DM S. cerevisiae. Therefore it could be concluded that the addition of S. cerevisiae up to 1 g/kg DM rations showed health improvement and able to produce low cholesterol broiler meat.

scholarly journals Effects of graded levels of Tetracin® on physico-chemical and sensory properties of broiler meat 1

2020 ◽  
Vol 44 (2) ◽  
pp. 34-45
Author(s):  
A. O. Akinwumi ◽  
A. A. Odunsi ◽  
A. B. Omojola ◽  
I. O. Olatoye ◽  
T. O. Akande ◽  
...  
Keyword(s):  
Crude Protein ◽  
Chemical Properties ◽  
Cooking Loss ◽  
Broiler Chicks ◽  
Feeding Trial ◽  
Breast Meat ◽  
Broiler Meat ◽  
Treatment Groups ◽  
Physico Chemical ◽  
Veterinary Antibiotic

Tetracin® (a feed grade veterinary antibiotic) was administered at 0, 50, 100, 150 and 200mg/kg to conventional starter and finisher feed for broilers for a period of six weeks before the breast meat were analyzed for physical, chemical and organoleptic properties. One hundred and fifty (2 weeks old) broiler chicks were randomly divided into 10 birds per replicate with 3 replicates per treatment. At the end of the feeding trial, 6 birds per treatment were slaughtered, defeathered, eviscerated and dressed. The breasts cuts were however subjected to laboratory analyses. No statistically significant (P>0.05) differences were observed in drip loss, chilling loss, shear force, ash and moisture contents of broiler meat across the treatment groups. However, the cooking loss and thermal loss progressively increased (P<0.05) with the corresponding increase in Tetracin®. Similarly, WHC was also significantly (P<0.05) influenced with increased inclusion of Tetracin®. Crude protein increased (P<0.05) but ether extract was reduced with inclusion of Tetracin®. Meat without antibiotics was highly rated (P<0.05) for flavour, juiciness and tenderness while colour and general acceptability were not significantly (P>0.05) influenced with or without Tetracin®. Conclusively, the inclusion of Tetracin® improved the chemical properties of broiler meat but the physical properties (cooking loss and WHC), flavour, juiciness and tenderness of the meat were compromised especially when administered above 100mg/kg feed.

scholarly journals Effect of turmeric and garlic supplementation to fermented Sauropus androgynus-bay leaves containing diet on fat deposition and broiler meat composition

2020 ◽  
Vol 45 (2) ◽  
pp. 91-102
Author(s):  
U. Santoso ◽  
Y. Fenita ◽  
K. Kususiyah ◽  
A. Agustian
Keyword(s):  
Fatty Acid ◽  
Eicosenoic Acid ◽  
Fat Deposition ◽  
Feed Additive ◽  
Broiler Meat ◽  
Treatment Groups ◽  
Commercial Feed ◽  
Acid Ratio ◽  
Amino Acid Profiles ◽  
Formulated Feed

This study aimed to examine the effect of turmeric and garlic inclusion to fermented Sauropus androgynus-bay leaves (FSBL) containing diet on fat deposition, and chemical composition of broiler meats. Two hundred 15-day-old female broilers were distributed into 5 treatment groups with 4 replications as follows. Control feeds with commercial feed additive (T0); Feeds with 1.25% FSBL (T1); Feed with FSBL plus 1 g turmeric (T2); Feed with FSBL plus 2 g garlic (T3); Feed with FSBL plus 1 g of turmeric and 2 g garlic (T4). It was shown that the fat deposition in the abdomen was significantly affected (P<0.01). Furthermore, the inclusion of turmeric and garlic to FSBL containing diet did not affect fat, protein, moisture and ash contents, but increased arginine, methionine and histidine (P<0.01). The addition of turmeric and/or garlic to the FSBL containing diet significantly influenced lauric acid, myristic acid, linolenic acid, cis-11-eicosenoic acid, cis-4,7,10,13,16,19-docosahexaenoic acid (P<0.05), n-3 fatty acid and n-6/n-3 fatty acid ratio (P<0.01). In conclusion, turmeric plus garlic supplementation improved amino acid profiles and changed fatty acid profiles. The possible interaction should be considered when we formulated feed additive from medicinal plant mixtures

scholarly journals Penambahan Air Jeruk Sambal (Citrus microcarpa) pada Air Minum terhadap Kadar Lemak Daging Broiler

2018 ◽  
Vol 18 (1) ◽  
pp. 18-23
Author(s):  
Rizqa Adisti ◽  
Duta Setiawan ◽  
Zakiatulyaqin Zakiatulyaqin ◽  
Marjoko Purnomosidi
Keyword(s):  
Analysis Of Variance ◽  
Abdominal Fat ◽  
Broiler Meat ◽  
Randomized Design ◽  
Commercial Feed ◽  
Completely Randomized Design

ABSTRAK. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan air jeruk sambal (Citrus microcarpa) pada air minum terhadap penurunan kadar lemak dan taraf lemak daging broiler. Materi yang digunakan adalah ayam umur 1 hari (DOC) strain CP 707 sebanyak 80 ekor yang ditempatkan dalam kandang berukuran (5,5 × 2,5 × 2 m) yang disekat menjadi 20 unit dengan setiap unit percobaan di isi 4 ekor broiler. Pakan yang digunakan adalah pakan komersial (BR10, BR11 dan BR12). Metode yang digunakan adalah rancangan acak lengkap (RAL), dengan 4 perlakuan dan 5 ulangan dengan taraf  perlakuan P0  (0% jeruk sambal per 1 L air ), P1 (0,4% jeruk sambal per 1 L air), P2 (0,8% jeruk sambal per 1 L air) dan P3 (1,2% jeruk sambal per 1 L air). Data yang diperoleh dianalisis menggunakan analisis ragam (ANOVA)  pada taraf  5% dilanjutkan dengan uji beda nyata jujur (BNJ). Hasil penelitian menunjukan bahwa perlakuan dapat meningkatkan persentase bobot karkas, penurunan persentase lemak abdominal dan persentase lemak daging broiler. Kesimpulan penambahan air jeruk sambal pada air minum dapat menurunkan lemak daging broiler dengan taraf  pemberian terbaik 0,8%.(The effect of native orange water (Citrus microcarpa) in drink water on broiler meat fat)ABSTRAK. This research aim to determine the effect of native orange (Citrus microcarpa) to drink water on percentage meat fat broiler and the best level for degrade meat fat. The used materials were day old chick (DOC) strain CP707 as many as 80 birds, were placed in cages with size of (5,5 ×2,5×2 m) is partitioned into 20 units an each unit in the experiment with the contents of 4 chickens. Commercial feed was  used, they were  (BR10, BR11 and BR12). Completely randomized design (CRD) was applied, with 4 treatment and 5 replications. The  level of treatments were  P0  (0% native orange for 1 L water), P1 (0,4 native orange for 1 L water), P2 (0,8% native oramge for 1 L water) and P3 (1,2% native orange for 1 L water). Data were analyzed using analysis of variance at level 5% and followed by honesty significant different (HSD).The result showed that there were increasing  percentage of carcass,  abdominal fat and meat fat. As a conclusion,  increment native orange to drink water can degrade broiler meat fat with the best level  at  0,8%.

scholarly journals Effect of Sauropus androgynus leaf extract, fish oil and vitamin E on performance, egg quality and composition in laying hens

2017 ◽  
Vol 42 (2) ◽  
pp. 88
Author(s):  
U. Santoso ◽  
Y. Fenita ◽  
E. Sulistyowati
Keyword(s):  
Vitamin E ◽  
Fish Oil ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Cholesterol Content ◽  
Leaf Extracts ◽  
Treatment Groups ◽  
Low Cholesterol ◽  
Β Carotene

The objective of this study was to obtain enriched eggs with low cholesterol content. Forty-eight laying hens (strain Dekalb Warren) aged 72 weeks were distributed into six treatment groups as follows: 1) Laying hens fed diet without Sauropus androgynus leaf extracts (SALE), fish oil and vitamin E (P0) as the control; 2) Laying hens were fed diet with 27 g SALE/kg diet, but without fish oil (P1); 3) Laying hens were fed diet with 27 g SALE/kg diet, 1.5% fish oil (P2); 4) Laying hens were fed diet with 27 g SALE/kg diet, 1.5% fish oil and 60 mg vitamin E/kg diet (P3); 5) Laying hens were fed diet with 27 g SALE/kg diet, 3% fish oil (P4); 6) Laying hens were fed diet with 27 g SALE/kg 3% fish oil and 60 mg vitamin E/kg diet (P5). Experimental results showed that the treatments affected (P <0.05) egg production, thick eggshell, egg white height, yolk color, the odor of eggs after the split (P <0.05). It was shown that the treatments had no effect on the contents of protein, fat, β-carotene of eggs but affected the contents of cholesterol and vitamin E of eggs. In conclusion, the treatments improved egg quality and increased egg Vitamin E but reduced egg cholesterol.

scholarly journals PENGARUH PEMBERIAN MIKROKAPSUL MINYAK IKAN TERHADAP LEMAK ABDOMEN DAN KADAR KOLESTEROL DAGING BROILER

2019 ◽  
Author(s):  
Toni Malvin ◽  
Mirzah ◽  
Montesqrit
Keyword(s):  
Fish Oil ◽  
Energy Content ◽  
Coconut Oil ◽  
Cholesterol Content ◽  
Abdominal Fat ◽  
Fat Percentage ◽  
Treatment Control ◽  
Broiler Meat ◽  
Cholesterol Levels ◽  
Randomized Design

This study was to determine the use of fish oil microcapsules in rations on the percentage of abdominal fat and cholesterol levels in broiler meat. The study used 100 DOC broilers with CP 707 code. The rations used consisted of rice bran, milled corn, soybean meal, fish meal, coconut oil, fish oil microcapsules (MMI), topmix and dolomite containing 22% protein with an energy content of 3000 Kcal / kg. The research method used Completely Randomized Design (CRD) with 4 treatments and 5 replications. The first treatment (R1) is containing 0.8% fish oil (control), the second treatment (R2) contains MMI 2%, the third treatment (R3) containing MMI 4%, and the fourth treatment (R4) containing 6% MMI. The parameters measured were the percentage of abdominal fat (%) and meat cholesterol (mg / 100g). The results of the analysis of variance that showed not significant effect for abdominal fat percentage until the use of MMI is 6%. As for meat cholesterol content, R2 and R3 treatment showed a very significant different effect (P&lt;0.01) lower than R1 treatment (control), and R4 treatment showed significant differences (P&lt;0.05) lower than R1 treatment (control), while between treatments R2, R3 and R4 showed no significant effect (P&gt;0.05). Based on the results of this study, it can be concluded that the use of MMI (fish oil microcapsules) at a level of 2% in broiler rations can have a significantly different effect on meat cholesterol content than controls, whereas for abdominal fat percentage the effect is not significant until the use of MMI is 6%.

Responses of different strains of Australian broiler chickens to feed restriction at an early age

1992 ◽  
Vol 43 (6) ◽  
pp. 1253 ◽  
Author(s):  
I Plavnik ◽  
D Balnave
Keyword(s):  
Broiler Chickens ◽  
Abdominal Fat ◽  
Feed Restriction ◽  
Meat Production ◽  
Early Age ◽  
Breast Meat ◽  
Broiler Meat ◽  
Commercial Broiler ◽  
Different Strains ◽  
Ad Libitum Feeding

Male chickens from the three most common commercial broiler strains in Australia were fed conventionally or on a regimen which restricted growth by approximately 58% between 6 and 12 days of age followed by ad libitum feeding. Early age feed restriction had no significant effect on bodyweight at 47 days of age, and feed was utilized significantly more efficiently with all three strains. Restriction significantly reduced abdominal fat and had no significant effect on the yield of breast meat. The large improvements in feed efficiency and reductions in abdominal fat have major implications with regard to the economics of broiler meat production.

scholarly journals Study on indiscriminate use of antibiotics in poultry feed and residues in broilers of Mymensingh city in Bangladesh

2019 ◽  
Vol 29 (4) ◽  
pp. 345-352 ◽  
Author(s):  
MI Khan ◽  
J Ferdous ◽  
MRA Ferdous ◽  
MS Islam ◽  
K Rafiq ◽  
...  
Keyword(s):  
Health Hazard ◽  
Age Groups ◽  
Poultry Feed ◽  
Growth Promoter ◽  
Antibiotic Residues ◽  
Breast Meat ◽  
Broiler Meat ◽  
Commercial Feed ◽  
Live Poultry ◽  
Antibiotic Residue

Abstract Knowledge, attitude and practice of broiler sellers about the indiscriminate use of antibiotic in poultry feed and antibiotic residue status of broiler in the live bird market of Mymensingh city was investigated. Twenty live poultry selling shops were randomly interviewed and six broilers from each market (5x6= 30) were randomly selected for detection of residual antibiotics in the liver and breast muscle. All the broiler meat sellers were male and the number of participants appeared higher in 21-30 year age group. The proportion of different age groups found to vary significantly (P<0.01). Among them, 10% sellers were illiterate, 60% sellers merely cross primary education, 25% sellers had secondary education and 5% sellers reached level of higher education. All sellers were familiar with antibiotics and vitamins. About 57.5% and 22.5% of the broiler sellers had knowledge onto the use of antibiotic as growth promoter. Only 7.5% sellers understood the term antibiotic resistance and 12% had little knowledge on human health hazard following indiscriminate use of antibiotics. All broiler sellers provided commercial feed and water throughout selling period, whereas, 77.5% and 17.5% of them used mixed vitamins and antibiotics in water to prevent unwanted mortality. In terms of adding extra antibiotics in feed, 32.5% of the sellers depended on feed sellers and only 20% of them used prescription provided by the registered veterinarian. The residue of ampicillin, ciprofloxacin and enrofloxacin in liver and meat of broilers were evaluated by using thin layer chromatography (TLC). The highest percentages of antibiotic residues were detected in the birds of Kachijhuli bazar (26.67%) and lowest in Shankipara bazar (13.33%). The highest percentages of antibiotics used in poultry feed was enrofloxacin (46.67%) followed by ciprofloxacin (30.00%) and amoxicillin (23.33%). In addition, amoxicillin plus ciprofloxacin (30%) and ciprofloxacin plus enrofloxacin (43.33%) were commonly found in the liver of broilers. All the broilers had antibiotic residue in their liver and breast meat containing antibiotic residues in 20% cases. Amoxicillin, ciprofloxacin and enrofloxacin were routinely used in poultry feed and the meat sellers had little knowledge about the indiscriminate use of antibiotics in poultry sector. Therefore, community base awareness regarding the discriminate and indiscriminate use of antibiotics in poultry sector is essential, and to evaluate the effect of residual antibiotics in the broilers onto the resistance of common bacterial pathogens living in the gut & systems. Progressive Agriculture 29 (4): 345-352, 2018

Effect of Escherichia Coli Infection on Metabolism of Dietary Protein in Intestine

2020 ◽  
Vol 21 (8) ◽  
pp. 772-776
Author(s):  
Xiao-Pei Peng ◽  
Wei Ding ◽  
Jian-Min Ma ◽  
Jie Zhang ◽  
Jian Sun ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Intestinal Tract ◽  
Effector Proteins ◽  
Physical Injury ◽  
Dietary Proteins ◽  
Pathogenic Role ◽  
E Coli ◽  
Low Protein ◽  
Pathogenic Escherichia Coli ◽  
Escherichia Coli Infection

Dietary proteins are linked to the pathogenic Escherichia coli (E. coli) through the intestinal tract, which is the site where both dietary proteins are metabolized and pathogenic E. coli strains play a pathogenic role. Dietary proteins are degraded by enzymes in the intestine lumen and their metabolites are transferred into enterocytes to be further metabolized. Seven diarrheagenic E. coli pathotypes have been identified, and they damage the intestinal epithelium through physical injury and effector proteins, which lead to inhibit the digestibility and absorption of dietary proteins in the intestine tract. But the increased tryptophan (Trp) content in the feed, low-protein diet or milk fractions supplementation is effective in preventing and controlling infections by pathogenic E. coli in the intestine.

scholarly journals Competitive Exclusion Reduces Transmission and Excretion of Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Broilers

2017 ◽  
Vol 83 (11) ◽  
Author(s):  
Daniela Ceccarelli ◽  
Alieda van Essen-Zandbergen ◽  
Bregtje Smid ◽  
Kees T. Veldman ◽  
Gert Jan Boender ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Competitive Exclusion ◽  
Intervention Strategy ◽  
Substantial Reduction ◽  
Poultry Production ◽  
Broiler Meat ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum ◽  
Probiotic Product

ABSTRACT Extended-spectrum β-lactamases (ESBLs) and plasmid-mediated AmpC β-lactamases (pAmpC) are enzymes able to hydrolyze a large variety of β-lactam antibiotics, including third-generation cephalosporins and monobactams. Broilers and broiler meat products can be highly contaminated with ESBL- and pAmpC-producing Escherichia coli strains, also known as extended-spectrum cephalosporin (ESC)-resistant E. coli strains, and can be a source for human infections. As few data on interventions to reduce the presence of ESC-resistant E. coli in broilers are available, we used transmission experiments to examine the role of competitive exclusion (CE) on reducing transmission and excretion in broilers. A broiler model to study the transmission of ESC-resistant E. coli was set up. Day-old chickens were challenged with an ESBL-producing E. coli strain isolated from healthy broilers in the Netherlands. Challenged and not challenged chicks were housed together in pairs or in groups, and ESBL-producing E. coli transmission was monitored via selective culturing of cloacal swab specimens. We observed a statistically significant reduction in both the transmission and excretion of ESBL-producing E. coli in chicks treated with the probiotic flora before E. coli challenge compared to the transmission and excretion in untreated controls. In conclusion, our results support the use of competitive exclusion as an intervention strategy to control ESC-resistant E. coli in the field. IMPORTANCE Extended-spectrum β-lactamases (ESBLs) and plasmid-mediated AmpC β-lactamases are a primary cause of resistance to β-lactam antibiotics among members of the family Enterobacteriaceae in humans, animals, and the environment. Food-producing animals are not exempt from this, with a high prevalence being seen in broilers, and there is evidence pointing to a possible foodborne source for human contamination. We investigated the effect of administration of a commercial probiotic product as an intervention to reduce the amount of ESBL-producing Escherichia coli in broilers. Our results showed a substantial reduction in the level of colonization of broiler intestines by ESBL-producing E. coli after administration of commercial probiotic product. The protective effect provided by these probiotics could be implemented on a larger scale in poultry production. Reductions in the levels of ESBL-producing Enterobacteriaceae in the food chain would considerably benefit public health.

Ability ofEscherichia coliO157:H7 isolates to colonize the intestinal tract of conventional adult CD1 mice is transient

2001 ◽  
Vol 47 (1) ◽  
pp. 91-95 ◽  
Author(s):  
J Wayne Conlan ◽  
Sonia L Bardy ◽  
Rhonda KuoLee ◽  
Ann Webb ◽  
Malcolm B Perry
Keyword(s):  
Escherichia Coli ◽  
Mouse Model ◽  
Intestinal Tract ◽  
Vaccine Development ◽  
Escherichia Coli O157 ◽  
Intestinal Colonization ◽  
E Coli ◽  
Cd1 Mice ◽  
A Current

In an attempt to improve upon a current mouse model of intestinal colonization by Escherichia coli O157:H7 used in this laboratory for vaccine development, nine clinical isolates of the pathogen were screened for their ability to persist in the intestinal tract of conventional adult CD-1 mice. None of the test isolates of E. coli O157:H7 were capable of colonizing these mice for a period of more than two weeks. Most of the isolates appeared to be benign for the experimental host, but one isolate was lethal. This virulence correlated with the ability of the latter isolate to produce large quantities of Shiga-like toxin 2 in vitro.

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