scholarly journals PENELITIAN PERUBAHAN WARNA DAN ETILEN SELAMA PEMERAMAN DAUN TEMBAKAU KULTIVAR MADURA SERTA PENGARUHNYA TERHADAP MUTU TEMBAKAU RAJANGAN

2020 ◽  
Vol 7 (3) ◽  
pp. 93
Author(s):  
JOKO PURDIYANTO
Keyword(s):  
Carboxylic Acid ◽  
Nicotiana Tabacum ◽  
Colour Change ◽  
The Other ◽  
Tobacco Leaves ◽  
Ripening Process ◽  
The Third ◽  
Randomized Design ◽  
Completely Randomized Design

<p><strong>Colour alternation and et/tylene changes during ripening tobacco leaves cultivar Madura and its effect to the quality of slice Madura tobacco</strong></p><p>The colour change and elhylene production in the leaves of Madura tobacco (Nicotiana tabacum) during ripening was studied at "Labora¬ torium Biokimia Pusat Antar Universitas Pangan dan Gizi Universitas Gadjahmada Yogyakata" and "Laboratorium Inti dan Atom Jurusan I'isika, FMIPA Universitas Gadjahmada Yogyakata". The change of colour was measured with Chrommameter CR-200. while the production of ethylcne, ACC (1-aminocyclopropanc-l-carboxylic acid) and MACC (malonyl-ACC) were measured with Photoaccuslic Spectroscopy, from the sample of 100 kg tobacco leaves cv. Jepon grown from April to September 1995 in Plakpak, Pcgantenan, Pamekasan, East Java. The experiment was designed as a completely randomized design in three replicates. The leaves samples were taken randomly from the mature middle leaves Results showed that endogenous ethylcne production al the irst day al a level of 169 ppb. was able to initiate ripening. Conversion percentage of ACC to ethylcne for 8 days ripening increased from 7.7 up lo 61.4 %. F tests least signiicantly different (1%). showed that yellow intensity signiicantly increased on the second day. This indicated thai ripening process was stated on the second day. On the other hand green intensity significantly decreased at the ifth day, thus no chlorophyl degradation. Brightness was signiicantly observed on Ihe third day of ripening, meaning that ripening stated on the third day. Based on the three criteria, the best ripening period was from the third lo the ilth day. This implies that the farmers will have three day periods of ripening which can be arranged to overcome the shotage of man power for slicing the leaves.</p>

Pengaruh Pemberian Moringa oleifera Terhadap Kuantitas dan Kualitas Semen Sapi Peranakan Ongole

2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus
Keyword(s):  
Data Analysis ◽  
Analysis Of Variance ◽  
Research Method ◽  
Moringa Oleifera ◽  
The Third ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design ◽  
Second Period

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen

scholarly journals PEMBERIAN STIMULAN PADA BIDANG SADAP DALAM MEMPENGARUHI PRODUKSI DAN KUALITAS LATEKS TANAMAN KARET (Hevea brasiliensis (Muell. Arg.)

2019 ◽  
Vol 4 (1) ◽  
pp. 38
Author(s):  
Hayata Hayata ◽  
Yulistiati Nengsih ◽  
Rahmanto Wibowo
Keyword(s):  
Quality Control ◽  
Ash Content ◽  
The Other ◽  
Rubber Content ◽  
Randomized Design ◽  
Treatment Factor ◽  
Completely Randomized Design ◽  
Four Levels ◽  
Latex Production

Production increasing of latex on rubber plants can be increased by using a stimulants. One of it  is Vicar 10 SL. The purpose of this study was to find out the effect of Vicar 10 SL on the production and quality of latex. This research was carried out in the Kasang Parit Village, Sekernan District, Muaro Jambi Regency, and the Goods Quality Control and Certification Center on Industry and trade department   in Jambi Province. The study was conducted in July to August 2017. The design used was a completely randomized design with one treatment factor as the Vicar 10 SL application with four levels of treatment, namely; Without treatment (V0/control), 1 ml/Tree (V1), 2 ml/tree (V2), 3 ml/tree (V3). There were 4 times  repeatation on each treatment. Using  fingers, a Vicar 10 SL solution was applied to the tapping groove which was adjusted to the treatment, and allowed to keep it 24 hours. Tapping is done in the next morning, by following the grooves and be stored in a cup and left until the latex stoped dripping. Vicar was given once a week during 30 days. Tapping was done 3 times a week. The variables observed were latex production (gram/tree/day), dry rubber content (%), latex ash content (%), and latex dirt content (%). Vicar giving 10 SL in the tapping site with a dose of 2 ml / tree gave the highest yield of latex production (93.38 grams / tree / day) and dry rubber content (75.50%) and was significantly different compared to the other treatments. Vicar giving 10 SL in tapping site had no significant effect on latex dirt content and latex ash contentKeywords: Latex, stimulant, product and quality Abstrak Peningkatan produksi lateks pada tanaman karet dapat ditingkatkan dengan menggunakan stimulan. Salah satu pemakaian yang digunakan adalah Vikar 10 SL. Tujuan penelitian ini adalah untuk megetahui penggunaan stimulan pada bidang sadap dalam mempengaruhi produksi dan kualitas lateks. Penelitian ini dilakukan di Desa kasang Parit Kecamatan Sekernan Kabupaten Muaro Jambi, dan Balai Pengujian dan Sertifikasi Mutu Barang (BPSMB) Disperindag Propinsi Jambi. Penelitian dilakukan pada bulan Juli sampai dengan Agustus 2017. Rancangan yang digunakan adalah Rancangan Acak Lengkap dengan satu faktor perlakuan pemberian stimulan (Vikar 10 SL) dengan empat taraf perlakuan  yaitu; Tanpa perlakuan (V0/kontrol),  1 ml/ Pohon (V1),  2 ml/pohon (V2), 3 ml/pohon  (V3). Setiap perlakuan diulang sebanyak 4 kali. Dengan menggunakan jari dioleskan larutan Vikar 10 SL pada alur sadap  yang disesuaikan dengan perlakuan, dan didiamkan selama 24 jam. Penyadapan dilakukan pada pagi hari esoknya, dengan mengikuti alur torehan dan ditampung dalam cawan dan dibiarkan sampai lateks berhenti menetes. Pemberian Vikar dilakukan sekali 7 hari selama 30 hari. Penyadapan dilakukan  3 kali dalam seminggu. Peubah yang diamati adalah produksi lateks (gram/pohon/hari)), kadar karet kering (%), kadar abu lateks (%), kadar kotoran lateks (%). Pemberian stimulan (Vikar 10 SL) pada bidang sadap dengan dosis 2 ml/pohon memberikan hasil yang tertinggi terhadap produksi lateks 93,38 gram/pohon/hari dan kadar karet kering 75,50 % dan berbeda nyata bila dibandingkan dengan perlakuan lainnya. Pemberian stimulan (Vikar 10 SL) pada bidang sadap berpengaruh tidak nyata terhadap kotoran lateks dan kadar abu lateksKata kunci :Lateks, stimulan, produksi dan kualitas

Skrining Fitokimia Enam Genotipe Teh

2014 ◽  
Vol 1 (2) ◽  
pp. 63
Author(s):  
Budi Martono ◽  
Rudi T. Setiyono
Keyword(s):  
Camellia Sinensis ◽  
Chemical Constituents ◽  
The Other ◽  
Preliminary Evaluation ◽  
Phytochemical Screening ◽  
Tea Leaves ◽  
Randomized Design ◽  
Medicinal Crops ◽  
Completely Randomized Design

<p>Skrining fitokimia dimaksudkan untuk melakukan evaluasi pendahuluan tentang kandungan kimia pada teh (Camellia sinensis). Selain itu, teh mengandung katekin yang dapat digunakan sebagai petunjuk kualitas dari daun teh. Penelitian bertujuan mengetahui kandungan senyawa aktif dan kadar katekin pada teh. Penelitian dilaksanakan mulai bulan April sampai dengan Juni 2012 di laboratorium Pengujian Balai Penelitian Tanaman Rempah dan Obat, Bogor. Skrining fitokimia pucuk peko dengan dua daun (p+2) dilakukan berdasarkan prosedur dari Materia Medika Indonesia (MMI), sedangkan analisis katekin dengan menggunakan metode SNI gambir. Penelitian disusun berdasarkan rancangan acak lengkap (RAL), enam perlakuan dengan empat ulangan. Perlakuan yang digunakan adalah enam genotipe teh (Tbs 1, Tbs 2, Hibrid, Cin 143, Rb 3, dan Kiara 8). Hasil penelitian menunjukkan keenam genotipe yang diuji mengandung senyawa alkaloid, saponin, tanin, fenolik, flavanoid, steroid, dan glikosida. Genotipe Tbs 1, Hibrid, dan Kiara 8 positif mengandung senyawa triterpenoid, sedangkan Tbs 2, Rb 3, dan Cin 143 negatif. Genotipe Tbs 1 dan Tbs 2 memiliki kandungan katekin paling tinggi (kecuali bagian ruas+tangkai daun) dibandingkan dengan empat genotipe lainnya. Pucuk peko, daun pertama, dan daun kedua pada genotipe Tbs 1 memiliki kadar katekin masing-masing 17,92%, 11,73%, dan 14,67%, sedangkan pada genotipe Tbs 2 masing-masing 18,22%, 13,48%, dan 15,81%. Kadar katekin terendah dihasilkan oleh bagian ruas+tangkai daun pada genotipe Rb 3 (1,78%). Pucuk peko menghasilkan kandungan katekin bervariasi antara 8,36%-18,22%, lebih tinggi dibandingkan dengan daun pertama, daun kedua, dan bagian ruas + tangkai daun.</p><p>Kata kunci: Camellia sinensis, fitokimia, genotipe, katekin, pucuk peko</p><p>Phytochemical screening was intended for a preliminary evaluation of the chemical constituents of the tea (Camellia sinensis). In addition, tea also contains catechin that can be used as an indication of the quality of tea leaves. The objectives of this study were to determine the content of the active compounds and catechin in tea. The research was conducted from April to June 2012 in the Laboratory of the Research Institute for Spices and Medicinal Crops, Bogor. The phytochemical screening was performed based on the procedure of Materia Medika Indonesia (MMI), while the catechin analysis used the method of SNI gambir. The study was carried out in completely randomized design with six treatments and four replications. The treatments used are six tea genotypes namely Tbs 1, Tbs 2, Hibrid, Cin 143, Rb 3, and Kiara 8. The results showed that the six tea genotypes tested contained the compounds of alkaloid, saponin, tannin, phenolic, flavanoid, steroid, and glycoside. Positively triterpenoid compounds present in the genotype of Tbs1, Hybrids, and Kiara 8, and negative in Tbs 2, Rb 3, and Cin 143. The genotypes of Tbs 1 and Tbs 2 produced the highest catechin content compared to the other genotypes. Catechin content was lowest in the part of internodes+leaf stalk of Rb 3 (1.78%). Pecco shoots produce catechin content of about 8.36%-18.22%, higher than the first leaf, second leaf, and the parts of internodes+leaf stalk.</p>

scholarly journals Kualitas Sosis Daging Ayam Dengan Penambahan Tepung Tapioka

2020 ◽  
Vol 5 (2) ◽  
pp. 64
Author(s):  
Bulkaini Bulkaini ◽  
Djoko Kisworo ◽  
Sukirno Sukirno ◽  
Rani Wulandani ◽  
Maskur Maskur
Keyword(s):  
Protein Content ◽  
Nutritional Value ◽  
Ash Content ◽  
The Other ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Tapioca Flour ◽  
Addition Level ◽  
Range Test

The study was aimed to determine the effect of tapioca flour addition atvarious levels on the quality of chicken sausage. The research was design based on a Completely Randomized Design (CRD) with 4 treatments and 3 replications. The treatment of tapioca flour addition level were: 0%, 10%, 20% and 30%. Data ofsausage quality were analyzed using Analysis of Variance and continued with Duncan's Multiple Range Test. The results showed that the addition of tapioca flour in the process of chicken sausage making were significantly affect (P <0.05) moisture and protein content, while the fat and ash content were not significantly effected (P> 0.05) by the treatment. The addition of 10% tapioca flour obtained the highest nutritional value of protein (17.67 ± 0.93) % as compared to the other levels.

scholarly journals Pengaruh Perbandingan Jenis Buah (Terong Belanda Dan Bit) dan Konsentrasi Carboxy Methyl Cellulose (CMC) Terhadap Mutu Organoleptik Sorbet

2016 ◽  
Vol 1 (1) ◽  
pp. 941-946
Author(s):  
Nanda Supriana ◽  
Dian Hasni ◽  
Syarifah Rohaya
Keyword(s):  
Concentration Factor ◽  
Methyl Cellulose ◽  
The Other ◽  
Carboxy Methyl Cellulose ◽  
Sour Taste ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Betalain Pigment ◽  
Carboxy Methyl

Abstrak.  Terong belanda merupakan buah berasa asam yang khas, namun memiliki warna yang kurang menarik. Disisi lain buah  bit mempunyai warna yang menarik, karena pada buah bit terkandung pigmen betalain yang dapat menghasilkan warna merah dan ungu. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh konsentrasi CMC dan penambahan buah terong belanda dan bit terhadap mutu organoleptik sorbet. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan faktor perbandingan buah terong belanda dan bit (P1 = 1:1, P2 = 2:1 dan P3 = 3:1) dan konsentrasi CMC ( C1 = 0%, C2 = 0,25%,  C3 = 0,50 % dan C4 = 75 %) dengan total  24 satuan percobaan. Hasil analisis menunjukkan penambahan proporsi terong belanda cenderung meningkatkan nilai organoleptik aroma dan rasa, sedangkan peningkatan konsentrasi CMC cenderung meningkatkan nilai organoleptik warna, rasa dan tekstur. Sorbet dengan perlakuan terbaik berdasarkan analisis keseluruhan diperoleh dengan menggunakan buah terong belanda:bit(2:1) dengan respon uji hedonik untuk warna 4,53 (agak suka), aroma 4,23 (netral), rasa 4,40 (agak suka) dan tekstur 4,63 (agak suka). Abstract. Terong belanda is a sour-taste typical fruit, but it has a less attractive color. On the other hand, beetroot has an interesting color because it contains betalain pigment producing red and purple colors. The purpose of this study was to determine the effect of CMC concentration and the addition of terong belanda and beetroot on the organoleptic quality of sorbet. This study used a Completely Randomized Design (CRD) with the ratio of terong belanda and beetroot factor (P1 = 1:1, P2 = 2:1 and P3 = 3:1) and CMC concentration factor (C1 = 0%, C2 = 0.25 %, C3 = 0.50% and C4 = 75%) with two replicates creating a total of 24 experimental units. The results showed that the addition of terong belanda tended to increase the proportion of aroma and taste organoleptic values, while the increase in the concentration of CMC tended to improve the color, taste and texture organoleptic values. The best Sorbet was obtained using the ratio of terong belanda and beetroot (2:1) having the following hedonic characteristics: 4.53 (a bit like) color, 4.23 (neutral) aroma, 4.40 (rather like) flavor and 4.63 (a bit like) texture.

Pengaruh Temperatur dan Lama Thawing terhadap Kualitas Spermatozoa Sapi dalam Penyimpanan Straw Beku

2010 ◽  
Vol 8 (1) ◽  
pp. 22
Author(s):  
S Utomo ◽  
E Boquifai
Keyword(s):  
Key Words ◽  
The Other ◽  
Effect Of Temperature ◽  
Randomized Design ◽  
Frozen Semen ◽  
Completely Randomized Design ◽  
Motility Of Sperm ◽  
Better Than

<p>This research was conducted to investigate the effect of temperature and duration of<br />thawing frozen semen in ministraw on quality sperm. Seventy five ministraws frozen semen of Simmental breed were thawing by water with temperature 5, 26 and 37 °C, with duration of 5, 10, 15, 20 and 30 second. The quality sperm post thawing was measured based on pH and motility. The design of this research was completely randomized design with factorial clasification 3 x 5. The result showed that the motility of sperm was thawing 37°C better than the other temperature. The quality of sperm was no affected by temperature and duration of thawing. It could be concluded<br />that thawing of frozen semen will have better done at 37°C with duration of 15 second.</p><p>Key words : Thawing, sperm, ministraw, frozen semen.</p>

scholarly journals Analisis Lemak Susu Edamame dari Olahan Biji Edamame

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Nufan Muwafiq Sukiran ◽  
Hari Santoso ◽  
Ahmad Syauqi
Keyword(s):  
Fat Content ◽  
The Other ◽  
Soy Milk ◽  
Average Value ◽  
The Third ◽  
Randomized Design ◽  
Completely Randomized Design

Edamame milk (Glycin max. L var. edamame) is one product with the advantages; easily produced and highly nutritious. This study aims was to determine the fat content of edamame milk prepared by edamame bean. The method used in this study is the experiment using a completely randomized design with 4 treatments and 5 replications. Fat analysis was done using the Gerber method. The fat levels of edamame milk at the first treatment, the average value of Edamame milk was 5.74% , the second 5.28%, the third 4.56% and the last treatment or the the fourth is fat at 2.0%. The results are compared with the other fat content of the other varieties, then it can be compared the fat content of Edamame varietes lower than the variants of Malika and Grobogan which are variants of Edamame fat content which is 5.74%, Malika fat content was 13.2%, and the Grobogan variance averaged 12.83%. The results of the analysis show that there are significant differences between treatments. For the average edamame fat content that is good is equal to 2.02% and the results close to the standard of soy milk that is equal to 2.0%.Keywords: fat content, edamame milk, Gerber method.ABSTRAKSusu edamame (Glycin max. L var. edamame) merupakan salah satu produk dengan kelebihan; mudah diproduksi dan bergizi tinggi. Penelitian ini bertujuan adalah menentukan kadar lemak susu edamame olahan biji Edamame. Metode yang digunakan dalam penelitian ini yaitu eksperimen dengan Rancangan Acak Lengkap dengan 4 perlakuan dan 5 kali ulangan. Analisis lemak dilakukan menggunakan metode Gerber. Hasil penelitian kadar lemak susu edamame pada perlakuan pertama, nilai rata-rata ulangan lemak susu edamame yaitu sebesar 5,74%, kedua 5,28% ketiga 4,56% dan keempat 2,02% Jika hasilnya dibandingkan dengan kadar lemak varietes lainnya maka bisa di bandingkan kadar lemak varietes edamame lebih rendah dari varietes malika dan grobogan yang mana varietes edamame kadar lemaknya yaitu sebesar 5,74%. Malika kadar lemaknya sebesar 13,2%, dan varietes Grobogan rata-ratanya sebesar 12,83%. Hasil analisis menunjukkan bahwa terdapat perbedaan yang signifikan diantara perlakuan. Rata-rata kadar lemak edamame yang baik yaitu sebesar 2,02%  dan hasilnya mendekati standar susu kedelai sebesar 2,0%Kata kunci: kadar lemak, susu edamame, metode Gerber.

scholarly journals The The Efectiveness of Jamblang (Syzygium cumini) Leaves Extract Inclusion Skim Milk-Egg Yolk Extender on Motility and Viability of Aceh Cattle Spermatozoa during Pre-Freezing and Post-Thawing

2021 ◽  
Vol 3 (3) ◽  
pp. p12
Author(s):  
Cut Intan Novita ◽  
Faqihuddin Nasution ◽  
Eka Meutia Sari
Keyword(s):  
Free Radicals ◽  
Skim Milk ◽  
Egg Yolk ◽  
The Other ◽  
Syzygium Cumini ◽  
Spermatozoa Motility ◽  
Randomized Design ◽  
Frozen Semen ◽  
Completely Randomized Design

The process of semen freezing causes an increase in free radicals concentration which can damage spermatozoa. The addition of natural ingredients in semen diluent is expected to solve this challenges. One of the natural ingredients that can be used is jamblang (Syzygium cumini) leaves. The objective of the current study was to investigate the quality of spermatozoa in Aceh cattle which was added with jamblang leaves extract in skim milk- egg yolk extender during pre-freezing and post-thawing. This study applied Completely Randomized Design (CRD) with 5 treatments and 5 replications. The treatments consisted of J0 = skim milk-egg yolk; J1 = skim milk-egg yolk + jamblang leaves extract 0.2%; J2 = skim milk-egg yolk + jamblang leaves extract 0.4%; J3 = skim milk-egg yolk + jamblang leaves extract 0.6%; and J4 = skim milk-egg yolk + jamblang leaves extract 0.8%. The parameters observed in this study were the percentage of motility and viability of frozen semen of Aceh cattle. The data obtained were analyzed using Analysis of Variance (ANOVA) and if differences were found, then it would be continued with Duncan's Multiple Distance test. The results showed that the addition of jamblang leaves extract in egg yolk skim milk significantly affected the percentage of motility during pre-freezing and post-thawing, significantly affected spermatozoa viability during pre-freezing and significantly affected the spermatozoa viability during post-thawing. J3 treatment (jamblang leaves extract 0.6 gram/100 ml) it should be higher than the other treatment, where the percentage of motility at pre-freezing and post-thawing were 55.48% and 52.71%, respectively, and the percentage of viability during pre-freezing and post-thawing were 56.59% and 53.94%, respectively. It was concluded that the addition of jamblang leaves extract in the skim milk-egg yolk extender affected the percentage of spermatozoa motility and viability of Aceh cattle during pre-freezing and post-thawing.

scholarly journals Pengaruh Temperatur dan Lama Thawing terhadap Kualitas Spermatozoa Sapi dalam Penyimpanan Straw Beku

2010 ◽  
Vol 8 (1) ◽  
pp. 22
Author(s):  
S Utomo ◽  
E Boquifai
Keyword(s):  
Key Words ◽  
The Other ◽  
Effect Of Temperature ◽  
Randomized Design ◽  
Frozen Semen ◽  
Completely Randomized Design ◽  
Motility Of Sperm ◽  
Better Than

<p>This research was conducted to investigate the effect of temperature and duration of<br />thawing frozen semen in ministraw on quality sperm. Seventy five ministraws frozen semen of Simmental breed were thawing by water with temperature 5, 26 and 37 °C, with duration of 5, 10, 15, 20 and 30 second. The quality sperm post thawing was measured based on pH and motility. The design of this research was completely randomized design with factorial clasification 3 x 5. The result showed that the motility of sperm was thawing 37°C better than the other temperature. The quality of sperm was no affected by temperature and duration of thawing. It could be concluded<br />that thawing of frozen semen will have better done at 37°C with duration of 15 second.</p><p>Key words : Thawing, sperm, ministraw, frozen semen.</p>

scholarly journals Artificial Autotetraploidy Induction Possibility of Two Iranian Endemic Mint (Mentha mozaffarianii) Ecotypes

2014 ◽  
Vol 6 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Askar GHANI ◽  
Seyyed Hossein NEAMATI ◽  
Majid AZIZI ◽  
Mohammad Jamal SAHARKHIZ ◽  
Mohammad FARSI
Keyword(s):  
Apical Meristem ◽  
Colchicine Treatment ◽  
Factorial Experiment ◽  
The Other ◽  
Chromosome Counts ◽  
Soaking Time ◽  
The Third ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design

The present study was conducted to polyploidy possibility induction of two Iranian endemic mint (Mentha mozaffarianii) ecotypes. For this purpose, three experiments were done. The first experiment was factorial, based on completely randomized design with three factors and three replications that rhizomes were used for treatment. The first factor including different colchicine concentrations (0, 0.025, 0.05, 0.1 and 0.2% that 0 as control). The second factor including two Mentha ecotypes (Ecotype A: Kamarej region and Ecotype B: Pirmohlat region) and the third factors consist of two soaking time (6 h and 12 h). In second experiment, apical meristem was treated. The factorial experiment based on randomized completely design with two factors and five replications. The factors including different colchicine concentrations (0, 0.035, 0.07, 0.15, 0.3 and 0.7%) and two ecotypes. In the third experiment, colchicine as combined with irrigation was applied. For this purpose, a factorial experiment in randomized complete design with two factors (colchicine concentrations: 0, 0.025, 0.012, 0.006% and two mint ecotypes) and three replications was conducted. At the end of all experiments, survival rate and tetraploidy percentage (by morphological change, stomata traits, flow cytometry and chromosome counts) were measured. The results showed that different treatment had significant effects on survival percent on all experiments and increasing of colchicine concentration caused decreasing plants survival. On the other hand, tetraploidy changes only in the first experiments were observed. Only in 0.025% colchicine treatment with 6 h soaking time on ecotype A, 12.66% tetraploidy was identified. Totally, it is seems that Mentha mozaffarianiihardly response to colchicine for tetraploidy induction.

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