scholarly journals Effect of Aqueous and Ethanolic Extracts of Tribulus terrestris, Phoenix dactylifera and Nasturtium officinale Mixture on Some Reproductive Parameters in Male Mice

2012 ◽  
Vol 9 (4) ◽  
pp. 640-650 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Ethanolic Extract ◽  
Phoenix Dactylifera ◽  
Tribulus Terrestris ◽  
Reproductive Parameters ◽  
Male Mice ◽  
Nasturtium Officinale ◽  
Ethanolic Extracts ◽  
Dose Dependent

The present investigation was conducted to evaluate the effect of the crude extracts mixture of three plants (Tribulus terrestris, Phoenix dactylifera and Nasturtium officinale) on semen quality,sex hormones and reproductive performance of mature male mice. A group of 25 male mice given 150mg/kg/day of the powder of the plants mixture with the food for four weeks and another three groups of 25 animals each given intraperitoneal injection from each of the aqueous and ethanolic extracts with a doses 75, 150, and 300mg/kg/day for two weeks. A remarkable increase in sperm concentration and motility with a decreased abnormal morphology was obtained in the experimental groups. A significant increase in hormones level were recognized in most groups. The results of mating untreated females with treated males of the four experimental groups revealed a decreased gestation period and an increased litter size. The results showed a dose dependent pattern of activity and the effect of the extracts were enhanced with increasing the dose level. The ethanolic extract being the more effective extract in all parameters.

scholarly journals Ethanolic extract of ocimum kilimandscharicum linnleaves: phytochemical and in vitro pharmacological activity

2021 ◽  
pp. 106-109
Author(s):  
Yamini N ◽  
Lahari S ◽  
Phani deepthi V
Keyword(s):  
In Vitro Model ◽  
Ethanolic Extract ◽  
Clinical Development ◽  
Dependent Manner ◽  
Thrombolytic Activity ◽  
Standard Drug ◽  
Ethanolic Extracts ◽  
Vitro Model ◽  
Dose Dependent

Using an in vitro model, the anti-thrombolytic efficacy of ethanolic extracts of Ocimum kilimandscharicum Linn was investigated. The researchers discovered that different concentrations of the extract had significant anti-thrombolytic activity in a dose-dependent manner , which was comparable to a standard drug. As a result of the presence of flavonoids and polyphenols in the plant extract, it can be concluded that it has a promising future in the treatment of thrombosis. This knowledge will be useful in the clinical development of thrombolytic therapeutics by identifying more potent anti-thrombolytic principles from natural resources..    

scholarly journals Comparison of hepatoprotective effect of aqueous and ethanolic extracts of Berberis lycium Royale (Sumbloo) in isoniazid induced hepatotoxicity in male mice model

2020 ◽  
Vol 9 (6) ◽  
pp. 835
Author(s):  
Saima Rafique ◽  
Khalida Ajmal ◽  
Uzma Naeem ◽  
Akbar Waheed ◽  
Ayesha Afzal ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Hepatoprotective Effect ◽  
High Dose ◽  
Dependent Manner ◽  
Male Mice ◽  
Mice Model ◽  
Ethanolic Extracts ◽  
Berberis Lycium

Background: The objective of the study was to compare the hepatoprotective effect of aqueous and ethanolic extracts of stem bark of Berberis lycium Royle in isoniazid (INH) induced hepatotoxicity in mice model.Methods: This randomized controlled in-vivo study conducted in male mice model from 10th April 2014 till 10th May 2014 at National Institute of Health, Islamabad. Group (C) was given normal diet and water. Group (D) was given isoniazid (50 mg/kg BW) to produce hepatotoxicity. Group (LA) and (HA) were given isoniazid (INH) plus low and high dose of aqueous extract of stem bark of Berberis lycium Royle respectively. Group (L.E) and (H.E) were given INH plus low and high dose of ethanolic extract of stem bark of Berberis lycium Royle respectively.Results: Hepatotoxicity produced by INH was shown by raised serum liver function tests (LFT’s), marked hepatocytic ballooning, significant steatosis and inflammation. Mice receiving simultaneous treatment of INH, low and high dose of aqueous extract of Berberis lycium Royle showed decrease serum LFT’s and their liver sections showed improved histological picture but more significant reduction in hepatotoxic effects were observed in animals receiving low and high doses of ethanolic extract.Conclusions: Hepatotoxicity of INH can be more fully reversed by simultaneous use of INH with ethanolic extract as it has better hepatoprotective potential in dose dependent manner as compare to aqueous extract of stem bark of Berberis lycium Royle.

scholarly journals TheIn VitroEffects of Aqueous and Ethanolic Extracts of the Leaves ofAgeratum conyzoides(Asteraceae) on Three Life Cycle Stages of the Parasitic NematodeHeligmosomoides bakeri(Nematoda: Heligmosomatidae)

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
J. Wabo Poné ◽  
Olivia Fossi Tankoua ◽  
Jeannette Yondo ◽  
Marie Claire Komtangi ◽  
Mpoame Mbida ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Larval Survival ◽  
Ageratum Conyzoides ◽  
Larval Stages ◽  
Life Cycle Stages ◽  
Ethanolic Extracts ◽  
Heligmosomoides Bakeri ◽  
Negative Controls ◽  
Dose Dependent

A comparativein vitrostudy was carried out to determine the ovicidal and larvicidal activity of aqueous and ethanolic extracts ofAgeratum conyzoides(Asteraceae) leaves on the eggs (unembryonated and embryonated), first and second larval stages ofHeligmosomoides bakeri. Four different concentrations (0.625, 1.25, 2.5, and 3.75 mg·mL−1) of both aqueous and ethanolic extracts were tested. Distilled water and 5% tween were used as negative controls in the bioassay. In fact, they did not affect development of eggs, hatching, and larval survival. The extract activities were dose dependent. The ethanolic extract was more potent against embryonation (39.6 ± 2.9%) than the aqueous extract (53.3 ± 10.9%) at the highest concentration (3.75 mg·ml−1). Both types of extracts killed larvae. Mebendazole proved more lethal (EC50of 0.745 and 0.323 mg·mL−1, resp., for L1and L2larvae). The aqueous extracts were the least lethal (EC50of 4.76 and 2.29 mg·mL−1, resp., for L1and L2larvae). The ethanolic extracts showed intermediate activity (EC50of 1.323 and 1.511 mg·mL−1, resp., for L1and L2larvae). It is concluded that the ovicidal and larvicidal properties of aqueous and ethanolic extracts ofAgeratum conyzoidesleaves are demonstrated in this work.

scholarly journals Evaluation of Anthelmintic Activity of Calendula officinalis Flowers Extract

2021 ◽  
Vol 11 (4) ◽  
pp. 48-50
Author(s):  
Alok Bhardwaj ◽  
Khursheed Alam ◽  
Devender Pathak ◽  
Shahid Ansari ◽  
Vandana Arora Sethi
Keyword(s):  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Chemical Investigation ◽  
Calendula Officinalis ◽  
Pharmacological Activities ◽  
Ethanolic Extracts ◽  
Level Of Significance ◽  
Dose Dependent ◽  
One Way Anova

Background: The present study is an attempt to explore the anthelmintic activity of ethanolic extract of flower of Calendula officinalis. Various doses of ethanolic extracts were evaluated for their anthelmintic activity on adult Indian earthworms, Pheretima posthuma. Calendula officinalis Linn has been widely used in homeopathic medicine for the treatment of many diseases. It has been reported to possess many pharmacological activities, which include antibacterial, antifungal, antiviral,antioxidant and anti-inflammatory. Methods: The ethanol extract of air dried flowers powder (500g) was prepared by using soxlet apparatus, concentrated and vacuum dried which gave an orange yellowish mass (60.46 g). Extract was subjected to qualitative chemical investigation of phytoconstituents such as glycosides, alkaloids, flavonoids, carbohydrates, tannins, proteins, vitamins, coumarins etc. Results: All extracts were able to show anthelmintic activity at 10mg/mL concentration. The activities are comparable with the standard drugs, piperazine citrate and albendazole. The data were found statistically significantly by using one way Anova at 5% level of significance (p< 0.05). Conclusion: All the doses of ethanolic extract of calendula officinalis showed dose dependent anthelmintic activity in comparison to standard drugs. Keywords: Calendula officinalis, Asteraceae, Anthelmintic, Piperazine citrate, Albendazole

scholarly journals Antinociceptive Effect of the Crude Ethanolic Extract of Crataeva nurvala. Buch. on Mice

1970 ◽  
Vol 4 (1) ◽  
pp. 65-68 ◽  
Author(s):  
MA Alam ◽  
ME Haque ◽  
JA Shilpi ◽  
KA Daulla
Keyword(s):  
Diclofenac Sodium ◽  
Antinociceptive Effect ◽  
Ethanolic Extract ◽  
Test Method ◽  
Inflammatory Conditions ◽  
Chemically Induced ◽  
Ethanolic Extracts ◽  
Experimental Animal Study ◽  
Pain Stimuli ◽  
Dose Dependent

In order to scientifically appraise some of the anecdotal, folkloric, ethnomedical uses of (Bengali name - borun), the present study was undertaken to investigate the antinociceptive properties of the ethanolic extracts of Crataeva nurvala on mice in the Phytochemistry and Pharmacology Laboratory, Pharmacy Discipline, Khulna University, Khulna, during the period from December 2003 to February 2004. The antinociceptive effect of crude ethanolic extracts was evaluated by ‘acetic acid' analgesic test method in mice. Crude ethanolic extracts of Crataeva nurvala (250-500 mg/kg PO) produced dose-dependent, significant (p < 0.05-0.001) antinociceptive effect against chemically induced nociceptive pain stimuli in mice. The results obtained in this study suggest that the antinociceptive effect of the extracts of Crataeva nurvala are peripherally and centrally-mediated. The findings of this experimental animal study indicate that crude ethanolic extracts of Crataeva nurvala possesses antinociceptive properties and thus lend pharmacological support to folkloric, anecdotal uses of ‘borun' in the treatment and/or management of painful, arthritic inflammatory conditions. Key Words: Antinociceptive, Crataeva nurvala, diclofenac sodium, writhing  doi:10.3329/bjvm.v4i1.1528 Bangl. J. Vet. Med. (2006). 4 (1): 65-68

scholarly journals Antibacterial screening of Phoenix dactylifera L. (Date palm) seed extracts on some bacterial isolates associated with dental caries

Bio-Research ◽  
2020 ◽  
Vol 18 (2) ◽  
Author(s):  
MR Adedayo ◽  
AE Ajiboye ◽  
FE Adetula
Keyword(s):  
Antibacterial Activity ◽  
Dental Caries ◽  
Date Palm ◽  
Ethanolic Extract ◽  
Phoenix Dactylifera ◽  
Diffusion Method ◽  
Bacterial Isolates ◽  
Phoenix Dactylifera L ◽  
Ethanolic Extracts ◽  
Dental Diseases

Dental diseases refer to conditions of deterioration and disintegration of the oral cavity due to the activities of pathogenic microbes. This study was carried out to determine the antibacterial activity of aqueous and ethanol extracts of Phoenix dactylifera L. (Date palm) seed on selected bacteria isolates associated with dental caries namely Staphylococcus aureus, Streptococcus mutans and Lactobacillus casei. Bioactive components of the seed were extracted using water and ethanol as solvents. The antibacterial activity of the extracts was examined by agar well diffusion method. The minimum inhibitory and bactericidal concentrations (MIC and MBC) were also determined using standard methods. All extracts were active against the tested isolates at the concentrations examined. L. casei was the most susceptible organism followed by S. mutans. S. aureus was the least susceptible. Ethanolic extract was more effective than aqueous extract at all the concentrations tested in the study. The MIC of aqueous and ethanolic extracts was 20 mg/ml while the MBC was 80 mg/ml respectively against all the bacterial isolates tested. It can be concluded that the aqueous and ethanolic extracts of the seed possess antibacterial potential against all the test organisms and could be useful in treatment of dental diseases and other related diseases caused by the bacteria isolates tested in the study.

scholarly journals Effets Antihypertensifs Des Extraits Aqueux Et Éthanolique Des Graines Fermentées De Parkia Biglobosa (Mimosaceae) Chez Les Rats

2017 ◽  
Vol 13 (36) ◽  
pp. 162
Author(s):  
Coulibaly Seydou Ouolouho ◽  
Ouattara Abou ◽  
Ouattara Karamoko ◽  
Coulibaly Adama
Keyword(s):  
Blood Pressure ◽  
Ethanolic Extract ◽  
Mineral Elements ◽  
Parkia Biglobosa ◽  
Traditional Use ◽  
Cardiovascular Parameters ◽  
Ethanolic Extracts ◽  
Dose Dependent Decrease ◽  
Extraits Aqueux ◽  
Dose Dependent

High blood pressure is a major cardiovascular risk factor in the occurrence of stroke, heart failure, kidney failure, and coronary heart disease. They are regarded as the leading causes of death worldwide. The aim of this study was to provide a scientific and pharmacological basis for the traditional use of Parkia biglobosa in the traditional treatment of arterial hypertension. Here, we first looked for certain mineral elements in the fermented seeds of P. biglobosa "soumara". Subsequently, we evaluated the hypotensive and antihypertensive effects of the aqueous and ethanolic extracts from these fermented seeds of P. biglobosa in normotensive and hypertensive rats. The cardiovascular parameters studied were systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR). The results showed that "soumara" is rich in calcium, potassium and magnesium, but low in sodium. Also, plant extracts at doses between 1000 and 2000 mg / kg bw induced a dose-dependent decrease in cardiovascular parameters in healthy rats. The treatment of rats made hypertensive with adrenaline by the ethanolic extract at a dose of 1500 mg / kg bw induced a dose-dependent decrease in cardiovascular parameters until normalization. The aqueous and ethanolic extracts of “soumara” acted in the same way as Nifédipine®, which is a calcium channel blocker.

Dose-Dependent Effect of Short-Term Repeated Exposure to Amitraz on Some Reproductive Parameters in Male Albino Rats

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Seriki Samuel Adinoyi
Keyword(s):  
Sperm Concentration ◽  
Control Group ◽  
Albino Rats ◽  
High Dose ◽  
Reproductive Parameters ◽  
Albino Rat ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Dose Dependent

Amitraz is a pesticide used in agriculture and public health to control insects, weeds, animals, and vectors of disease. Although the use of pesticides is of benefit in general, abuse of the pesticides is harmful due to their potential toxicity to humans and animals. Present study examined the effect of the pesticide on the male reproductive parameters of the male albino rats. 20 rats were grouped into four. Group 1 was control, Group 2 was given low dose Amitraz, Group 3 was median dose, and Group 4 was high dose. The administration was for a period of 21 days. Results showed that Amitraz has dose dependent toxicity effect on the reproductive parameter of the rat. Amitraz is toxic to the reproductive parameters of the albino rat, and could lower sperm concentration, sperm motility, and sperm morphology. By implication, Amitraz is toxic to the reproductive system and could cause infertility in male.

Biological and Phytochemical Screening of Tephrosia purpurea extract on Chemically Induced Hepatocellular Carcinoma with Reference to Biochemical Parameters

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Irfan Aziz ◽  
Birendra Shrivastava ◽  
Chandana Venkateswara Rao ◽  
Sadath Ali
Keyword(s):  
Free Radicals ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Marker Enzymes ◽  
Tephrosia Purpurea ◽  
Single Intraperitoneal Injection ◽  
Chemically Induced ◽  
Ccl4 Treatment ◽  
Dose Dependent

Tephrosia purpurea possesses hepatoprotective activity as evidenced by the significant and dose dependent restoring the activities of entire liver cancer marker enzymes, diminution in tumor incidence, decrease in lipid peroxidation (LPO) and increase in the level of antioxidant enzymes (GSH, CAT, SOD, GPx and GST) through scavenging of free radicals, or by enhancing the activity of antioxidant, which then detoxify free radicals. These factors protect cells from ROS damage in NDEA and CCl4-induced hepatocarcinogenesis. Histopathological observations of liver tissues too correlated with the biochemical observations. Thus, present investigation suggested that the Tephrosia purpurea would exert a chemoprotective effect by reversing the oxidant-antioxidant imbalance during hepatocarcinogenesis induced by NDEA and CCl4. Besides Tephrosia purpurea is very much effective in preventing NDEA-induced multistage hepatocarcinogenesis possibly through antioxidant and antigenotoxic nature, which was confirmed by various liver injury and biochemical tumour markers enzymes. The hepatoprotective activity of aTephrosia purpurea of 50 % ethanolic extract was studied using rats. The animals received a single intraperitoneal injection of N-nitrosodiethylamine 200mg/kg body wt followed by subcutaneous injection of CCl4 in a dose of 3 ml/kg body wt.Tephrosia purpureaextract dose dependently and significantly the increase in serum hepatic enzyme levels after NDEAand CCl4 treatment compared to the toxin control group. The results of this study confirmed the antioxidant and hepatoprotective activity of the Tephrosia purpurea extract against carbon tetrachlorideand N-nitrosodiethylamine induced hepatotoxicity in rats.

Restraint stress of male mice triggers apoptosis in spermatozoa and spermatogenic cells via activating the TNF-α system

Zygote ◽  
2020 ◽  
Vol 28 (2) ◽  
pp. 160-169 ◽  
Author(s):  
Jie Zhang ◽  
De-Ling Kong ◽  
Bin Xiao ◽  
Hong-Jie Yuan ◽  
Qiao-Qiao Kong ◽  
...  
Keyword(s):  
Psychological Stress ◽  
Restraint Stress ◽  
Semen Quality ◽  
Sperm Quality ◽  
Fertilization Rate ◽  
Seminiferous Tubules ◽  
Spermatogenic Cells ◽  
Male Mice ◽  
Testicular Cells ◽  
Tnf Α

SummaryStudies have indicated that psychological stress impairs human fertility and that various stressors can induce apoptosis of testicular cells. However, the mechanisms by which psychological stress on males reduces semen quality and stressors induce apoptosis in testicular cells are largely unclear. Using a psychological (restraint) stress mouse model, we tested whether male psychological stress triggers apoptosis of spermatozoa and spermatogenic cells through activating tumour necrosis factor (TNF)-α signalling. Wild-type or TNF-α−/− male mice were restrained for 48 h before examination for apoptosis and expression of TNF-α and TNF receptor 1 (TNFR1) in spermatozoa, epididymis, seminiferous tubules and spermatogenic cells. The results showed that male restraint significantly decreased fertilization rate and mitochondrial membrane potential, while increasing levels of malondialdehyde, active caspase-3, TNF-α and TNFR1 in spermatozoa. Male restraint also increased apoptosis and expression of TNF-α and TNFR1 in caudae epididymides, seminiferous tubules and spermatogenic cells. Sperm quality was also significantly impaired when spermatozoa were recovered 35 days after male restraint. The restraint-induced damage to spermatozoa, epididymis and seminiferous tubules was significantly ameliorated in TNF-α−/− mice. Furthermore, incubation with soluble TNF-α significantly reduced sperm motility and fertilizing potential. Taken together, the results demonstrated that male psychological stress induces apoptosis in spermatozoa and spermatogenic cells through activating the TNF-α system and that the stress-induced apoptosis in spermatogenic cells can be translated into impaired quality in future spermatozoa.

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