scholarly journals Disentangling potential genotypes for macro and micro nutrients and polymorphic markers in Chickpea

2021 ◽  
Author(s):  
Neha Mittal ◽  
Juhi Bhardwaj ◽  
Shruti Verma ◽  
Rajesh Kumar Singh ◽  
Renu Yadav ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Genetic Resources ◽  
Block Design ◽  
Polymorphic Markers ◽  
Breeding Strategies ◽  
Similarity Coefficients ◽  
Nutritional Properties ◽  
Potential Donor ◽  
Fasta Format ◽  
Randomized Block Design

Abstract Background- The present investigation was conducted to assess the nutritional diverseness and identify novel genetic resources to be utilized in chickpea breeding for macro and micro nutrients. Methods-The plants were grown in randomized block design. Nutritional and phytochemical properties of nine chickpea genotypes were estimated. The EST sequences from NCBI database were downloaded in FASTA format, clustered into contigs using CAP3, mined for novel SSRs using TROLL analysis and primer pairs were designed using Primer 3 software. Jaccard’s similarity coefficients were used to compare the nutritional and molecular indexes followed by dendrograms construction employing UPGMA approach. Results- The genotypes PUSA-1103, K-850, PUSA-1108, PUSA-1053 and the EST-SSR markers ICCeM012, ICCeM0049, ICCeM0070, ICCeM0078, SVP55, SVP95, SVP96, SVP146, SVP213 & SVP217 were found as potential donor / marker resources for the macro-micro nutrients. The genotypes differed (p<0.05) for nutritional properties. Amongst newly designed primers, 6 were found polymorphic with median PIC (0.46). The alleles per primer ranged 1 to 8. Cluster analysis based on nutritional and molecular diversities partially matched to each other in principle. Conclusion-The identified novel genetic resources may be used to widen the germplasm base, prepare maintainable catalogue and identify systematic blueprints for future chickpea breeding strategies targeting macro-micro nutrients.

scholarly journals Identification of donor lines for improving fruit yield of k 35 x k 12 eggplant hybrid

Genetika ◽  
2004 ◽  
Vol 36 (1) ◽  
pp. 61-67 ◽  
Author(s):  
Jelena Damnjanovic ◽  
Maja Vracarevic ◽  
Gordana Surlan-Momirovic ◽  
Slaven Prodanovic
Keyword(s):  
Inbred Line ◽  
Block Design ◽  
Field Trials ◽  
Fruit Yield ◽  
Potential Donor ◽  
Modified Method ◽  
Randomized Block Design ◽  
Donor Line ◽  
K 12 ◽  
Method Of Evaluation

The aim of this study was to identify an eggplant donor line which possess the largest frequency of favourable alleles that control fruit yield. Such donor line should be used to improve the elite eggplant hybride K35 x K12. The fruit yield of the elite hybrid, its parents and their hybrids with three potential donor lines (K36/1, K11 and K22/2) was examined in a diallel set by means of field trials. The trials were set in a randomized block design in three replications. After the fruit yield had been measured, the modified method of evaluation of relative loci value according to Dudley (1987) was applied. Ali inbred lines expressed positive values of the ?G' parameter. The K11 inbred had the largest ?G' value (2.38) and, also, the lowest frequency of unfavourable alleles on the loci class D (1.33). It was established that the K11 inbred line was more related to the K35 parental inbred line (7.81). Consequently, on the basis of the ?D' values, improvement should be obtained by backcrossing the elite hybrid K35 x K11 to the donor inbred K11.

Integration of Various Stability Models to Identify High Yielding and Stable Genotypes of Pigeonpea [Cajanus cajan (L.) Millspaugh]

2021 ◽  
Author(s):  
Amit Kumar Gaur ◽  
S.K. Verma ◽  
R.K. Panwar ◽  
Anju Arora
Keyword(s):  
Cluster Analysis ◽  
Seed Yield ◽  
Hierarchical Cluster Analysis ◽  
Cajanus Cajan ◽  
Block Design ◽  
Hierarchical Cluster ◽  
Interaction Effects ◽  
Kharif Season ◽  
Pulse Crop ◽  
Randomized Block Design

Background: Pigeonpea is second most important pulse crop of India after chickpea and it is necessary to identify its high yielding and stable genotypes to feed the increasing population of country. Methods: The present study was laid down in a Randomized Block Design with three replications during kharif season of 2016-2019 at GBPUAT, Pantnagar using twenty genotypes of pigeonpea with an aim to identify the high yielding and stable genotypes. The hierarchical cluster analysis (HCA) based on mean seed yield and Average of Sum of Ranks (ASR) of all measures (Parametric and non-parametric) was used in present study.Result: The pooled ANOVA revealed the presence of significant differences among genotypes, environments and G x E interaction effects. The results of hierarchical cluster analysis indicated the genotypes PA 622 (yield=1774.85 kg/ha, ASR=2.00), PA 620 (yield= 1579.92 kg/ha, ASR=2.18), UPAS 120 (yield=1268.57 kg/ha, ASR=2.87), PA 626 (yield=1571.40 kg/ha, ASR=5.56), PUSA 992 (yield= 1331.17 kg/ha, ASR=5.68) and PA 628 (yield= 1271.50 kg/ha, ASR=7.06) as most stable and high yielding and hence these genotypes can be recommended for pigeonpea improvement programmes.

Cluster and canonical variate analyses in multilocation trials of linseed

2003 ◽  
Vol 140 (3) ◽  
pp. 297-304 ◽  
Author(s):  
W. ADUGNA ◽  
M. T. LABUSCHAGNE
Keyword(s):  
Cluster Analysis ◽  
Block Design ◽  
Canonical Variate Analysis ◽  
Canonical Variate ◽  
Good Seed ◽  
Randomized Block Design ◽  
Variate Analysis ◽  
Testing Environments ◽  
Multivariate Cluster ◽  
Multilocation Trials

Multivariate cluster and canonical variate analyses were undertaken for 10 genotypes of linseed (Linum usitatissimum L.) that were tested in a four-times replicated randomized block design across 18 environments (six localities by 3 years) of Ethiopia. The main aims of this study were to determine the similarities and differences of the genotypes and their testing environments, and to compare applicability of the two statistical methods. Cluster analysis grouped the genotypes into five classes in accordance with their original sources. The six locations and 18 environments were stratified into four and seven clusters, respectively. Three sites (Bekoji, Kulumsa and Sinana) were separately stratified, while three other ones (Holetta, Asasa and Adet) showed closer similarity. Canonical variate analysis indicated that ‘D33C’ and ‘D24C’ were distinguished from the other genotypes by their high oil contents. ‘N10D’ and ‘Norlin’ had closer values and were thus preferred for their good seed yield and earliness. Days to flowering and maturity, oil contents and lodging per cent played major roles in discriminating the genotypes. Comparison of the two methods showed clearer differentiation by cluster analysis than canonical variate analysis. Canonical variate analysis also contributed information on how each variable discriminated the genotypes and their test environments. Thus, both methods complement each other in providing useful information for more efficient variety development programmes.

scholarly journals EARLY GROWTH AND SEEDLING MORPHOLOGY OF SPECIES OF Sesbania Scop. (Leguminosae, robinieae)

1999 ◽  
Vol 56 (2) ◽  
pp. 329-335 ◽  
Author(s):  
Elisabeth Ann Veasey ◽  
Marta Organo Negrão ◽  
Paulo Sodero Martins ◽  
Gerhard Bandel
Keyword(s):  
Cluster Analysis ◽  
Intraspecific Variation ◽  
Block Design ◽  
Group Method ◽  
Seedling Morphology ◽  
Low Frequencies ◽  
Initial Development ◽  
Arithmetic Average ◽  
Pair Group ◽  
Randomized Block Design

The objective of this study was to characterize 17 accessions of Sesbania, representing S. exasperata, S. grandiflora, S. sesban, S. tetraptera and S. virgata at the seedling stage, and to evaluate the initial development during the first two months after planting. The trial was conducted in a greenhouse in a randomized block design, with 4 replications and 5 plants per plot. The traits recorded were: plant height (PH), from four observations at 15-day intervals, at 17, 32, 47 and 62 days after planting; length of hypocotyl (LH) and epicotyl (LEP); length (LE1) and width (WE1) of the first eophyll; and number of leaflet pairs of the second metaphyll (NLP), evaluated 17 days after planting. Univariate analyses of variance were performed, estimating the genetic parameters: coefficient of genotypic determination (b) and of genetic variation (CVg). Cluster analysis was also obtained, using the average Euclidean distance and Unweighted pair group method of arithmetic average (UPGMA) method. At 17 days after planting, S. exasperata presented the highest PH, followed by S. virgata. At 62 days after planting, S. sesban registered the highest PH. Length of hypocotyl displayed inter but not intraspecific variation. The characters LEP, LE1, WE1 and NLP showed both inter and intraspecific variation. Cluster analysis indicated the existence of 7 groups, separating the species and revealing intraspecific variation as well. The occurrence in low frequencies of two unifoliolate opposite eophylls for some species was observed, as well as bi- or trifoliolate first eophylls for one of the S. sesban accessions. These informations are basic for the selection of traits to be utilized for characterization and differentiation of Sesbania germplasm at the juvenile phase.

scholarly journals PRODUCTION OF STAR FRUIT ROOTSTOCKS FERTILIZED WITH DIFFERENT BORON DOSES

2017 ◽  
Vol 92 (3) ◽  
pp. 221
Author(s):  
Clariana Xavier ◽  
William Natale
Keyword(s):  
Experimental Design ◽  
Quality Index ◽  
Block Design ◽  
Dry Mass ◽  
Tropical Fruits ◽  
Nutritional Properties ◽  
Star Fruit ◽  
Randomized Block Design ◽  
Number Of Leaves ◽  
Dickson Quality Index

Among the tropical fruits, star fruit stands out due to its flavor and nutritional properties. Nevertheless, the information on fertilization and nutrition of this fruit is still scarce. For this reason, this study aimed to assess the effect of different boron doses on star fruit rootstock growth. The experimental design was randomized block design, consisting of five treatments of boron application (0, 1, 2, 3, and 4 mg dm−3) and four replications. Star fruit rootstock did not respond to boron doses applied to the substrate and hence it did not influence the parameters stem diameter, plant height, the number of leaves, dry mass of shoot, dry mass of roots, total dry mass, and Dickson quality index (DQI). A boron dose up to 4 mg dm−3 (800% of the recommended boron dose of 0.5 mg dm−3) did not produce symptoms of visual toxicity in the plant.

scholarly journals Stabilitas dan Adaptabilitas Varietas Padi Merah Lokal Daerah Istimewa Yogyakarta

2016 ◽  
Vol 16 (2) ◽  
pp. 103
Author(s):  
NFN Kristamtini
Keyword(s):  
Genetic Resources ◽  
Block Design ◽  
Yield Stability ◽  
Red Rice ◽  
Average Yield ◽  
Local Varieties ◽  
Genetic Potential ◽  
Randomized Block Design ◽  
The Stability ◽  
Special Region

<p>Stability and Adaptability of Local Varieties of Red Rice in Daerah Istimewa Yogyakarta. The objective of this research were to asses the yield performance, stability and adaptability of three genetic resources of local red rice in Yogyakarta Special Region. A randomized Block Design with three repicates was applied in all location. Three varieties were used i.e. Cempo merah, Saodah merah and Andel merah and were planted with changed plant system. Fertilizers were applied at rate 200 kg/ha urea, 50 kg/ha TSP and 25 kg/ha KCl. Yield stability was analyzed using the method of Eberhart and Russel (1966). The result indicated that the genetic resources test had different genetic potential. The yield of tested variety from 4.75-5.55 t/ha with an average of 5.08 t/ha. The variety of Cempo merah and Andel merah were under the average of yield stability and were only adapted to optimal growing environments. The stability of Saodah merah was higher than stability of average yield and was also well adapted to sub optimal environments.</p><p> </p><p><strong>Abstrak</strong></p><p>Tujuan penelitian ialah menilai daya hasil, stabilitas, dan adaptabilitas tiga varietas padi merah lokal Provinsi Daerah Istimewa Yogyakarta (DIY) di Sleman dan Bantul. Rancangan percobaan di setiap lokasi ialah acak kelompok dengan tiga ulangan. Tiga varietas yang diuji ialah Cempo merah, Saodah Merah, dan Andel Merah, ditanam dengan sistem tanam pindah. Pemupukan dengan 200 kg/ha urea, 50 kg/ha TSP, dan 25 kg/ha KCl. Stabilitas hasil dianalisis dengan metode Eberhart dan Russe (1966). Hasil penelitian menunjukkan terdapat perbedaan potensi genetik dari sumber daya genetik yang diuji. Kisaran hasil dari ketiga varietas padi merah lokal yang diuji antara 4,75-5,55 t/ha dengan rata-rata 5,08 t/ha. Varietas padi merah Cempo Merah dan Andel Merah memiliki stabilitas di bawah rata-rata, dan berpeluang adaptif di lingkungan optimal, sedangkan varietas Saodah Merah memiliki stabilitas di atas rata-rata dan beradaptasi pada lingkungan kurang optimal.</p>

scholarly journals Rendimiento forrajero y sus componentes según la frecuencia de corte de Moringa oleifera, variedad Criolla

2018 ◽  
Vol 29 (2) ◽  
pp. 425
Author(s):  
José Leonardo Ledea Rodríguez ◽  
Guiselle Rosell Alonso ◽  
Diocles Guillermo Benítez Jiménez ◽  
Ramón Crucito Arias ◽  
Jorge Valentín Ray Ramírez ◽  
...  
Keyword(s):  
Dry Matter ◽  
Moringa Oleifera ◽  
Animal Feed ◽  
Block Design ◽  
Cutoff Frequency ◽  
Forage Yield ◽  
Organic Fertilization ◽  
Nutritional Properties ◽  
Randomized Block Design ◽  
Number Of Leaves

In some countries of Central America, Moringa spp is considered as the tree of life because of its nutritional properties and its plasticity. In some of them, research is held to take advantage of Moringa spp foraging and nutritional properties regarding plant age, to be used for animal feed. This study aims to evaluate the effect of cutoff frequency on forage yield of Moringa oleifera, cultivar Criolla. The study was carried out in January and December 2012, in a Fluvisol soil of Cauto Valley under irrigation conditions and organic fertilization. A randomized block design with four replications was used and the variables considered were, plant height, number and thickness of shoots, number of leaves, leaf/stem ratio, total dry matter yield (DM), and leaf dry matter to explain the response of cutting at 45 and 60days. All the analyzed variables were significantly affected (p≤0.05) by cutoff frequency. The highest (p≤0,05) yield ofleaf dry matter , stems, and total were obtained when cutting at 60 days with 7.3, 11.8, and 18.4 t MS/ha respectively.It is concluded that cutting frequency modified the productive behavior of cultivars under study.

scholarly journals Isozyme Pattern and Morpho-agronomical Traits Based Genetic Divergence Studies in Maize (Zea mays L.) Inbreds

2019 ◽  
pp. 1-8
Author(s):  
Ranju Kumari ◽  
A. K. Singh ◽  
V. K. Sharma
Keyword(s):  
Genetic Divergence ◽  
Block Design ◽  
Inbred Lines ◽  
Isozyme Pattern ◽  
Starch Gel Electrophoresis ◽  
Similarity Coefficients ◽  
Dissimilarity Coefficient ◽  
Randomized Block Design ◽  
Cluster Distance ◽  
Agronomical Traits

The present investigation was carried out with an objective to study genetic divergence based on morpho-agronomical traits and isozyme pattern in eight maize inbreds. These inbreds were evaluated in randomized block design with three replication for ten morph-agronomical traits. Horizontal starch gel electrophoresis technique used to study the isozyme polymorphism in different tissues of eight inbreds. Analysis of variance revealed significant differences among the inbreds for all the ten morpho-agronomical traits. Nature and extent of genetic divergence for morpho-agronomical traits was measured using average taxonomic distances as a measure of dissimilarity coefficient. Eight inbreds were clustered into four groups (A, B, C, D) based on dissimilarity coefficient. Cluster B and cluster D showed the highest inter cluster distance (2.2422) and the lowest was observed between clusters B and C (1.0401). Cluster A exhibited the highest intra cluster distance (0.8519). Based on inter cluster distances inbreds present in cluster B and D were found more diverse  consisted of inbred CML 186 and CM 600 respectively. Six isozyme systems were used for characterization and divergence studies based on similarity coefficients. Inbreds were classified into six clusters (A, B, C, D, E and F). The lowest (0.5957) similarity coefficient exist between inbreds CM 600 and CML 176 and the highest (0.8132) existed between inbreds CML 186 and CML 144. Cluster analysis in both cases reflected the moderate level of genetic divergence among the inbred lines but result may not be completely similar, but somewhat distinct and complementary in nature. Isozyme patterns was found to effective in revealing the nature of relationship among the inbred lines Therefore, divergence study using one estimate can’t replace the need to evaluate the relationship on the basis of the other which may be to used as parents in hybridization programme.

Innovation of Natural Preservation of Mackerel (Rastrelliger kanagurta) Using Coconut Coconut Ashes with Drying Time Variations on Hedonics

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Rika Agustina ◽  
Rita Sunartaty ◽  
Teuku Makmur
Keyword(s):  
Research Design ◽  
Block Design ◽  
Drying Time ◽  
Basic Ingredient ◽  
Randomized Block Design ◽  
Salt Substitute ◽  
Long Time ◽  
Time Variations ◽  
Rastrelliger Kanagurta

Coconut frond ash is one of the wastes from coconut trees which has not been maximally utilized. Coconut frond ash contains MgCl2 and KCl so that it can be used as a salt substitute in the process of preserving fish. In this study coconut frond ash was used as a basic ingredient for making dried mackerel with a long time of drying to storage. The purpose of this study was to determine the effect of drying time on mackerel storage. The research design used was a Randomized Block Design (RCBD) with 2 factors studied. The first factor is the drying time consists of 3 levels, namely P1 = 3 days, P2 = 4 days, P3 = 5 days. The second factor is storage which consists of 3 levels, namely S1 = 30 days, S2 = 60 days, S3 = 90 days. Each treatment was repeated 2 times to obtain 18 experimental units to observed hedonic tests. From the results of the study it can be stated that the treatment has a very significant effect (P≥0.01) on the hedonic test which includes (color, aroma, taste and texture).

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

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