C-Reactive Protein Knock Out Attenuate Temporomandibular Joint Inflammation in Rats

Abstract Background: C-reactive protein (CRP), a biomarker of inflammation, is highly expressed in osteoarthritis (OA)-related diseases, but its exact role remains unknown. In this study, we evaluated the biological effect of CRP on temporomandibular joint (TMJ) inflammation.Methods: Freund’s complete adjuvant (CFA) was used to induce TMJ inflammation in CRP-knockout (CRP-/-) and control rats. Degenerative changes in the TMJ were compared to elucidate the role of CRP in TMJ inflammation. In addition, inflammatory cytokines, macrophage activation and osteoclast differentiation were evaluated by real-time quantitative polymerase chain reaction, immunohistochemistry and tartrate-resistant phosphatase staining to explore the potential regulatory mechanism.Results: Compared to the control, CFA induced TMJ inflammation, which increased systemic and local CRP expression. Furthermore, CRP-/- rats exhibited less severe inflammatory symptoms. The downregulation of proinflammatory cytokines (interleukin (IL)-1β and IL-6) and upregulation of the anti-inflammatory cytokine IL-10 were detected in CRP-/- rats, which also exhibited reduced macrophage activation and osteoclast differentiation.Conclusion: These results indicated that controlling the highly elevated levels of CRP during inflammation could modify the cytokine profile, macrophage activation and osteoclast differentiation, thus providing beneficial effects for TMJ-OA prevention and treatment.

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C-Reactive Protein Knock Out Attenuate Temporomandibular Joint Inflammation in Rats

10.21203/rs.3.rs-115377/v2 ◽

2021 ◽

Author(s):

Yao He ◽

Mengjiao Zhou ◽

Zixiang Jian ◽

Lingli Fang ◽

Lan Huang ◽

...

Keyword(s):

Temporomandibular Joint ◽

Macrophage Activation ◽

Quantitative Polymerase Chain Reaction ◽

Osteoclast Differentiation ◽

Joint Inflammation ◽

C Reactive Protein ◽

Knock Out ◽

Beneficial Effects ◽

Reactive Protein ◽

And Control

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C-Reactive Protein Knockout Attenuates Temporomandibular Joint Inflammation in Rats

Journal of Immunology Research ◽

10.1155/2022/8613986 ◽

2022 ◽

Vol 2022 ◽

pp. 1-20

Author(s):

Yao He ◽

Mengjiao Zhou ◽

Zixiang Jian ◽

Lingli Fang ◽

Lan Huang ◽

...

Keyword(s):

Temporomandibular Joint ◽

Macrophage Activation ◽

Quantitative Polymerase Chain Reaction ◽

Osteoclast Differentiation ◽

Joint Inflammation ◽

C Reactive Protein ◽

Beneficial Effects ◽

Reactive Protein ◽

And Control

Background. C-reactive protein (CRP), a biomarker of inflammation, is highly expressed in osteoarthritis- (OA-) related diseases, but its exact role remains unknown. In this study, we evaluated the biological effect of CRP on temporomandibular joint (TMJ) inflammation. Methods. Freund’s complete adjuvant (CFA) was used to induce TMJ inflammation in CRP-knockout (CRP-/-) and control rats. Degenerative changes in the TMJ were compared to elucidate the role of CRP in TMJ inflammation. In addition, inflammatory cytokines, macrophage activation, and osteoclast differentiation were evaluated by real-time quantitative polymerase chain reaction, immunohistochemistry, and tartrate-resistant phosphatase staining to explore the potential regulatory mechanism. Results. Compared to the control, CFA induced TMJ inflammation, which increased systemic and local CRP expression. Furthermore, CRP-/- rats exhibited less severe inflammatory symptoms. The downregulation of proinflammatory cytokines (interleukin- (IL-) 1β and IL-6) and upregulation of the anti-inflammatory cytokine IL-10 were detected in CRP-/- rats, which also exhibited reduced macrophage activation and osteoclast differentiation. Conclusion. These results indicated that controlling the highly elevated levels of CRP during inflammation could modify the cytokine profile, macrophage activation, and osteoclast differentiation, thus, providing beneficial effects for TMJ-OA prevention and treatment.

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C-Reactive Protein Knock Out Attenuate Temporomandibular Joint Inflammation in Rats

10.21203/rs.3.rs-115377/v1 ◽

2020 ◽

Author(s):

Yao He ◽

Mengjiao Zhou ◽

Zixiang Jian ◽

Lingli Fang ◽

Lan Huang ◽

...

Keyword(s):

Temporomandibular Joint ◽

Morphological Changes ◽

Osteoclast Differentiation ◽

Joint Inflammation ◽

Cartilage Degeneration ◽

Control Group ◽

C Reactive Protein ◽

Knock Out ◽

Reactive Protein ◽

Temporomandibular Joint Osteoarthritis

Abstract Background: C-reactive protein (CRP), as the biomarker for inflammation, high expresses in osteoarthritis (OA) related diseases with exact role been clouded. In this study, we evaluated the biological effect of CRP in temporomandibular joint osteoarthritis (TMJ-OA).Methods: Freund’s complete adjuvant (CFA) was used for TMJ inflammation induction in CRP knock out (CRP -/-) and control rats. And TMJ degenerative changes, such as the synovitis performance, TMJ disc morphological changes and cartilage degeneration were compared to elucidate the role of CRP played in TMJ-OA. Results: Compared to control group, CFA induced TMJ inflammation caused systemic and local CRP expression increased. Based on this, CRP -/- rat performed less severe inflammation symptoms. Lower degree of pro-inflammatory cytokines (interleukins IL-1β and IL-6) expression and up-expression of anti-inflammatory cytokine IL-10 were detected in CRP -/- rat, which with less macrophage and activation and osteoclast differentiation.Conclusion: These results indicated that control high elevated CRP level during inflammation should be benefit for TMJ-OA prevention and treatment.

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Monomeric C-Reactive Protein Binds and Neutralizes Receptor Activator of NF-κB Ligand-Induced Osteoclast Differentiation

Frontiers in Immunology ◽

10.3389/fimmu.2018.00234 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 6

Author(s):

Zhe-Kun Jia ◽

Hai-Yun Li ◽

Yu-Lin Liang ◽

Lawrence Albert Potempa ◽

Shang-Rong Ji ◽

...

Keyword(s):

Bone Erosion ◽

Osteoclast Differentiation ◽

Joint Inflammation ◽

Dependent Manner ◽

C Reactive Protein ◽

Native Conformation ◽

Reactive Protein ◽

Receptor Activator ◽

Binding Sequence

C-reactive protein (CRP) is an established marker of rheumatoid arthritis (RA) but with ill-defined actions in the pathogenesis. Here, we show that CRP regulates the differentiation of osteoclasts, a central mediator of joint inflammation and bone erosion in RA, in a conformation- and receptor activator of NF-κB ligand (RANKL)-dependent manner. CRP in the native conformation is ineffective, whereas the monomeric conformation (mCRP) actively modulates osteoclast differentiation through NF-κB and phospholipase C signaling. Moreover, mCRP can bind RANKL, the major driver of osteoclast differentiation, and abrogate its activities. The binding and inhibition of RANKL are mediated by the cholesterol binding sequence (CBS) of mCRP. Corroborating the in vitro results, CRP knockout exacerbates LPS-induced bone resorption in mice. These results suggest that mCRP may be protective in joint inflammation by inhibiting pathological osteoclast differentiation and that the CBS peptide could be exploited as a potential RANKL inhibitor.

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Serum C-reactive protein and WBC count in conservatively and operatively managed bacterial spondylodiscitis

Journal of Orthopaedic Surgery ◽

10.1177/2309499020968296 ◽

2020 ◽

Vol 29 (1) ◽

pp. 230949902096829

Author(s):

Christina van Gerven ◽

Kevin Eid ◽

Tobias Krüger ◽

Michael Fell ◽

Daniel Kendoff ◽

...

Keyword(s):

Antibiotic Treatment ◽

Serum Levels ◽

Blood Chemistry ◽

Nonoperative Treatment ◽

C Reactive Protein ◽

Disc Disease ◽

Reactive Protein ◽

Postoperative Serum ◽

Wbc Count ◽

And Control

Purpose: C-reactive protein (CRP) and white blood cell (WBC) count are routine blood chemistry parameters in monitoring infection. Little is known about the natural history of their serum levels in conservative and operative spondylodiscitis treatment. Methods: Pre- and postoperative serum levels of CRP and WBC count in 145 patients with spondylodiscitis were retrospectively assessed. One hundred and four patients were treated by debridement, spondylodesis, and an antibiotic regime, 41 only with a brace and antibiotics. The results of the surgical group were compared to 156 patients fused for degenerative disc disease (DDD). Results: Surgery had a significant effect on peak postoperative CRP levels. In surgically managed patients, CRP peaked at 2–3 days after surgery (spondylodiscitis: pre-OP: 90 mg/dl vs. post-OP days 2–3: 146 mg/dl; DDD: 9 mg/dl vs. 141 mg/dl; p < 0.001), followed by a sharp decline. Although values were higher for spondylodiscitis patients, dynamics of CRP values were similar in both groups. Nonoperative treatment showed a slower decline. Surgically managed spondylodiscitis showed a higher success rate in identifying bacteria. Specific antibiotic treatment led to a more predictable decline of CRP values. WBC did not show an interpretable profile. Conclusion: CRP is a predictable serum parameter in patients with spondylodiscitis. WBC count is unspecific. Initial CRP increase after surgery is of little value in monitoring infection. A preoperative CRP value, and control once during the first 3 days after surgery is sufficient. Closer monitoring should then be continued. Should a decline not be observed, therapy needs to be scrutinized, antibiotic treatment reassessed, and concomitant infection contemplated.

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Efficacy of Tocilizumab Therapy in Different Subtypes of COVID-19 Cytokine Storm Syndrome

Viruses ◽

10.3390/v13061067 ◽

2021 ◽

Vol 13 (6) ◽

pp. 1067

Author(s):

Oleksandr Oliynyk ◽

Wojciech Barg ◽

Anna Slifirczyk ◽

Yanina Oliynyk ◽

Vitaliy Gurianov ◽

...

Keyword(s):

Macrophage Activation Syndrome ◽

Macrophage Activation ◽

Cytokine Storm ◽

Cytokine Release ◽

Cytokine Release Syndrome ◽

C Reactive Protein ◽

Treatment Results ◽

Reactive Protein ◽

Median Serum ◽

D Dimer

Background: Cytokine storm in COVID-19 is heterogenous. There are at least three subtypes: cytokine release syndrome (CRS), macrophage activation syndrome (MAS), and sepsis. Methods: A retrospective study comprising 276 patients with SARS-CoV-2 pneumonia. All patients were tested for ferritin, interleukin-6, D-Dimer, fibrinogen, calcitonin, and C-reactive protein. According to the diagnostic criteria, three groups of patients with different subtypes of cytokine storm syndrome were identified: MAS, CRS or sepsis. In the MAS and CRS groups, treatment results were assessed depending on whether or not tocilizumab was used. Results: MAS was diagnosed in 9.1% of the patients examined, CRS in 81.8%, and sepsis in 9.1%. Median serum ferritin in patients with MAS was significantly higher (5894 vs. 984 vs. 957 ng/mL, p < 0.001) than in those with CRS or sepsis. Hypofibrinogenemia and pancytopenia were also observed in MAS patients. In CRS patients, a higher mortality rate was observed among those who received tocilizumab, 21 vs. 10 patients (p = 0.043), RR = 2.1 (95% CI 1.0–4.3). In MAS patients, tocilizumab decreased the mortality, 13 vs. 6 patients (p = 0.013), RR = 0.50 (95% CI 0.25–0.99). Сonclusions: Tocilizumab therapy in patients with COVID-19 and CRS was associated with increased mortality, while in MAS patients, it contributed to reduced mortality.

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Studying the Role of C-Reactive Protein in Patients with Sleeve Gastrectomy in Baghdad

Journal of Techniques ◽

10.51173/jt.v3i1.282 ◽

2021 ◽

Vol 3 (1) ◽

pp. 55-60

Author(s):

Mohammed Abbas Fadil ◽

Raya Ezat Maroof ◽

Moayed Abbas Fadil

Keyword(s):

Sleeve Gastrectomy ◽

Healthy Person ◽

Control Group ◽

C Reactive Protein ◽

Serum Samples ◽

Reactive Protein ◽

Medical City ◽

Healthy Control ◽

And Control

Obesity and severe obesity are increasing serious health problems with an epidemic percentage in most countries. In Sleeve gastrectomy, a part of the stomach structure is removed, limiting its capacity by about two to three. A total of thirty blood samples were collected from patients with obesity and the result was compared with healthy person throughout the time from November 2019 to September 2020. Before sleeve gastrectomy and after more than 6 months of sleeve surgery, the sample was collected from the Medical City/Baghdad Teaching Hospital, the withdrawal was again taken at home to have pre and post sleeve gastrectomy, Patient age ranged between [20-46] years for obese patients and healthy control. Then the serum samples were obtained from patients and control group to screen for C-reactive protein by agglutination method. The result of the present study found that the positivity of CRP in pre-operation is higher than that of post-operative with high significance [P<0.005].

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Abstract P625: Improvement of Autonomic Function and C-Reactive Protein in Military Personnel with Traumatic Stress After Use Of a Closed Loop Neurotechnology

Hypertension ◽

10.1161/hyp.68.suppl_1.p625 ◽

2016 ◽

Vol 68 (suppl_1) ◽

Author(s):

Hossam A Shaltout ◽

Catherine L Tegeler ◽

Charles H Tegeler

Keyword(s):

Heart Rate ◽

Traumatic Stress ◽

Closed Loop ◽

Brain Activity ◽

Renin Angiotensin System ◽

Acoustic Stimulation ◽

Total Power ◽

C Reactive Protein ◽

Beneficial Effects ◽

Reactive Protein

Objective: Evaluate changes in autonomic cardiovascular control and inflammatory markers associated with use of High-resolution, relational, resonance-based, electroencephalic mirroring (HIRREM) in subjects enrolled in a pilot study for symptoms of military-related traumatic stress (MTS). Introduction: Symptoms associated with MTS include insomnia, depression, anxiety, activated inflammatory response and impaired autonomic control. HIRREM is a noninvasive, closed-loop acoustic stimulation technology that identifies dominant brain frequencies and translates them in real time into audible tones of variable pitch and timing, to support self-updating and self-optimization of brain activity. Methods: Eighteen service members or Veterans (1 female), mean (SD) age 40.9 (7.0), with MTS symptoms for 6 years (3.4), received 19.5 (1.1) HIRREM sessions over 12 days. Continuous recordings of blood pressure and heart rate, for analysis of baroreflex sensitivity (BRS) and heart rate variability (HRV), were done before and immediately after completion of the HIRREM intervention. Blood samples were also collected (n = 14) for measurement of catecholamines, cytokines, C-reactive protein and the renin angiotensin system (RAS) components. Paired t-tests were performed. After HIRREM, there was improved BRS measured as HF alpha (9.6 ms/mmHg, SE 3.1, p = 0.005), Sequence Down (7.6 ms/mmHg, 2.4, p = 0.005), Sequence Up (8.4 ms/mmHg, 3.0, p = 0.01), and Sequence All (7.6 ms/mmHg, 2.2, p = 0.002), as well as HRV; SDNN (12.0 ms, 3.5, p = 0.02), rMSSD (13.2 ms, 3.0, p < 0.001), LF power (1023.0 ms 2 , 346, p = 0.007), HF power (398.0 ms 2 , 142.0, p = 0.01), and total power (1420.8 ms 2 , 450.8, p = 0.005). C-reactive protein (36%, p = 0.057) was also reduced. There were no significant changes in the catecholamine, cytokines or RAS. There were no adverse events or dropouts. Conclusions: These interim results suggest improved autonomic cardiovascular regulation, across multiple measures of BRS and HRV, and reduction in CRP associated with the use of HIRREM for symptoms of MTS. Confirmation of these results in a larger cohort may provide important insights regarding both the mechanisms associated with the beneficial effects of HIRREM, and the functional disturbances underlying MTS.

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Influence of Myeloperoxidase Levels on Periodontal Disease: An Applied Clinical Study

Applied Sciences ◽

10.3390/app10031037 ◽

2020 ◽

Vol 10 (3) ◽

pp. 1037

Author(s):

Alessandro Polizzi ◽

Salvatore Torrisi ◽

Simona Santonocito ◽

Mattia Di Stefano ◽

Francesco Indelicato ◽

...

Keyword(s):

Coronary Heart Disease ◽

Regression Analysis ◽

Multivariate Regression ◽

Multivariate Regression Analysis ◽

Healthy Controls ◽

Plasma Samples ◽

C Reactive Protein ◽

Periodontal Health ◽

Reactive Protein ◽

And Control

In this trial, we evaluated the influence on plasma and salivary myeloperoxidase (MPO) levels of periodontal health, coronary heart disease (CHD), periodontitis, or both periodontitis and CHD. Clinical and periodontal parameters were collected from periodontitis patients (n = 31), CHD patients (n = 31), patients with both periodontitis and CHD (n = 31), and from healthy patients (n = 31) together with saliva and plasma samples. The median concentrations of salivary and plasma MPO were statistically higher in the CHD patients [plasma: 26.2 (18.2–34.4) ng/mg; saliva 83.2 (77.4–101.5) ng/mL, p < 0.01] and in the periodontitis plus CHD patients [plasma: 27.8 (22.5–35.7) ng/mg; saliva 85.6 (76.5–106.7) ng/mL, p < 0.001] with respect to periodontitis and control patients. Through a univariate regression analysis, c-reactive protein (CRP) and CHD (both p < 0.001) and periodontitis (p = 0.024) were statistically correlated with MPO in plasma. The multivariate regression analysis demonstrated that only CRP was statistically the predictor of MPO in plasma (p < 0.001). The multivariate regression analysis in saliva demonstrated that, regarding MPO levels the only predictors were CRP (p < 0.001) and total cholesterol (p = 0.035). The present study evidenced that subjects with CHD and periodontitis plus CHD had higher plasma and salivary levels of MPO compared to subjects with periodontitis and healthy controls.

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