Effects of Different Straw Biochar Combined With Microbial Inoculants on Soil Environment of Ginseng

Abstract Ginseng is an important cash crop. The long-term continuous cropping of ginseng causes the imbalance of soil environment and the exacerbation of soil-borne diseases, which affects the healthy development of ginseng industry. In this study, ginseng continuous cropping soil was treated with microbial inocula using broad-spectrum biocontrol microbial strain Frankia F1. Wheat straw, rice straw and corn straw were the best carrier materials for microbial inoculum. After treatment with microbial inoculum prepared with corn stalk biochar, the soil pH value, organic matter, total nitrogen, available nitrogen, available phosphorus, and available potassium were increased by 11.18%, 55.43%, 33.07%, 26.70%, 16.40%, and 9.10%, the activities of soil urease, catalase and sucrase increased by 52.73%, 16.80% and 43.80%, respectively. A Metagenomic showed that after the application of microbial inoculum prepared fromwith corn stalk biochar, soil microbial OTUs, Chao1 index, Shannon index, and Simpson index increased by 19.86%, 16.05%, 28.83%, and 3.16%, respectively. Three classes (Alphaproteobacteria, Gammaproteobacteria and Sphingobacteria) were the dominant bacteria in ginseng soil, and their abundance increased by 7.87%, 9.81% and 1.24%, respectively, after treatment with microbial inoculum (corn stalk biochar). Results indicated that the most effective treatment in ginseng soil ould be the combined application of corn stalk biochar and Frankia F1.

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Effects of different straw biochar combined with microbial inoculants on soil environment in pot experiment

Scientific Reports ◽

10.1038/s41598-021-94209-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yuqi Qi ◽

Haolang Liu ◽

Jihong Wang ◽

Yingping Wang

Keyword(s):

Ph Value ◽

Shannon Index ◽

Available Phosphorus ◽

Continuous Cropping ◽

Soil Environment ◽

Corn Straw ◽

Available Nitrogen ◽

Combined Application ◽

Ginseng Soil ◽

Important Cash Crop

AbstractGinseng is an important cash crop. The long-term continuous cropping of ginseng causes the imbalance of soil environment and the exacerbation of soil-borne diseases, which affects the healthy development of ginseng industry. In this study, ginseng continuous cropping soil was treated with microbial inocula using broad-spectrum biocontrol microbial strain Frankia F1. Wheat straw, rice straw and corn straw were the best carrier materials for microbial inoculum. After treatment with microbial inoculum prepared with corn straw biochar, the soil pH value, organic matter, total nitrogen, available nitrogen, available phosphorus, and available potassium were increased by 11.18%, 55.43%, 33.07%, 26.70%, 16.40%, and 9.10%, the activities of soil urease, catalase and sucrase increased by 52.73%, 16.80% and 43.80%, respectively. A Metagenomics showed that after the application of microbial inoculum prepared with corn straw biochar, soil microbial OTUs, Chao1 index, Shannon index, and Simpson index increased by 19.86%, 16.05%, 28.83%, and 3.16%, respectively. Three classes (Alphaproteobacteria, Gammaproteobacteria and Sphingobacteria) were the dominant bacteria in ginseng soil, and their abundance increased by 7.87%, 9.81% and 1.24%, respectively, after treatment with microbial inoculum with corn straw biochar. Results indicated that the most effective treatment in ginseng soil would be the combined application of corn straw biochar and Frankia F1.

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Effects of Continuous Cropping of Dictyophora on Soil Physical and Chemical Properties, Microbial Biomass and Enzyme Activity

Journal of Innovation and Social Science Research ◽

10.53469/jissr.2021.08(07).39 ◽

2021 ◽

Vol 8 (7) ◽

pp. 214-220

Author(s):

Long Tong ◽

◽

Hongyan Li ◽

Xiaoming Liu ◽

Bin Li ◽

...

Keyword(s):

Enzyme Activity ◽

Ph Value ◽

Chemical Properties ◽

Available Phosphorus ◽

Continuous Cropping ◽

Physical And Chemical Properties ◽

Factors Affecting ◽

The One ◽

Physical And Chemical ◽

And Chemical Properties

The continuous obstacle of Dictyophora indusiata has become the one of the main factors affecting the healthy development of D. indusiata industry. In order to study the effects of continuous cropping of D. indusiata on the soil environment, four treatments were used in this study: no planted (CK), planted for 1 years (1Y), continuous cropping for 2 years (2Y) and continuous cropping for 3 years (3Y), to determined of the yield of D. indusiata, soil physical and chemical properties, microbial content and enzyme activity. The results showed that the yield and soil pH value decreased with the increase of continuous cropping years, and the contents of organic matter, total nitrogen, total phosphorus and total potassium, C/N and C/P also increased with the increase of continuous cropping years. Soil availability decreased with the increase of continuous cropping years, the content of alkaline nitrogen, available phosphorus and available potassium decreased by 12.25%, 28.91% and 24.86% at 3Y compared with 1Y, respectively. The biomass of bacteria, actinomycetes and fungi and the total amount of microorganism in soil increased at 1Y compared with CK, but with the increase of continuous cropping years, the biomass of bacteria and actinomycetes and the total amount of microorganism decreased significantly, while the biomass of fungi increased significantly; the continuous cropping of D. indusiata also decreased the value of bacteria/fungi in soil. The enzyme activities of the soil were higher than those of the unplanted plots, however, the activities of urease, catalase, peroxidase, sucrase, phosphatase and protease decreased with the increase of continuous cropping years. In a word, with the increase of continuous cropping, the acidity of rhizosphere soil increased, the availability of soil nutrients and the activity of soil enzymes decreased, the biomass of soil microorganisms, bacteria and actinomycetes decreased. However, the increase of fungal biomass led to the decline of soil texture.

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Vegetation Characteristics and Response to the Soil Properties of Three Medicinal Plant Communities in Altay Prefecture, China

Sustainability ◽

10.3390/su122410306 ◽

2020 ◽

Vol 12 (24) ◽

pp. 10306

Author(s):

Tao Lang ◽

Libo Pan ◽

Bo Liu ◽

Tong Guo ◽

Xikang Hou

Keyword(s):

Medicinal Plants ◽

Medicinal Plant ◽

Soil Properties ◽

Plant Communities ◽

Ph Value ◽

Available Phosphorus ◽

Available Nitrogen ◽

Nutrient Contents ◽

Positive Effects ◽

Local Soil

Plant communities and their environments are a vital part of synecology research. In this study, we investigated the characteristics of three typical medicinal perennial herbs, Glycyrrhiza uralensis, Rheum altaicum, and Ferula sinkiangensis, and examined their response to local soil properties in Altay Prefecture, China. A total of 29 species belonging to 15 families and 25 genera were recorded. In terms of the target medicinal plants, the importance value was low and community structures were exceedingly simple. Quantitative classification based on WARD cluster analysis suggested that each medicinal plant can be classified into one of three types: annual herbaceous, perennial herbaceous, and subshrub or shrub. Interestingly, the plants gradually transformed from exhibiting nonresistance to xerophytes or halophytes. An investigation of the soil properties beneath the medicinal plants showed that nutrients in black soil were much more abundant than those in sandy and saline soil. Except for leaching phenomenon, the nutrient contents in the soil surface layer (0–10 cm) were higher than those in deep layers (10–20 cm and 20–40 cm), and to some extent, the nutrient contents were inversely proportional to the depth of the soil. The redundancy analysis (RDA) results of the medicinal plants and their relationship with soil properties indicated that the main soil variables impacting the distribution of G. uralensis were available potassium (AK), aspect (ASP), soil moisture (SM), total nitrogen (TN), and total phosphorus (TP); SM, electric conductivity (EC), elevation (ELE), ASP, pH value (pH), available nitrogen (AN), soil organic content (SOC), and the carbon/nitrogen (C/N) ratio had positive effects on the distribution of R. altaicum. F. sinkiangensis grew better in habitats with high contents of SM, EC, TN, AN, available phosphorus (AP), total potassium (TK), and AK. This study aimed at providing effective suggestions for artificial cultivation, conservation, and resilience of Chinese medicinal plants.

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The Assessment of Human Activities Impact on Spatial Heterogeneity of Soil Environment - Taking DongPing Forest Park as an Example

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.573-574.331 ◽

2012 ◽

Vol 573-574 ◽

pp. 331-336

Author(s):

Shi Dong Liu ◽

Dong Qian Xue ◽

Jun Gao

Keyword(s):

Organic Matter ◽

Spatial Heterogeneity ◽

Bulk Density ◽

Available Phosphorus ◽

Soil Environment ◽

Available Nitrogen ◽

Forest Park ◽

Impact Research ◽

The Impact ◽

Background Area

Research of the impact of tourism activities on the soil has been a focus in domestic and foreign environmental impact research. Targeted at spatial heterogeneity that domestic and foreign soil environment impact research seldom touches, this paper took Dongping Forest Park on Shanghai Chongming Island as a sample land and made Chongming Forest Tourism Festival the major driving factor. Through comparative analysis of bulk density, organic matter of soil and soil available nutrient before and after the festival, the paper adopted PCA ranking to study the spatial distribution and law of heterogeneity of soil environment evolution in Dongping Forest Park under the impact of human tourism activities, and concluded that: (1) bulk density, organic matter, available nitrogen and available phosphorus showed regular changes, but the change of available potassium was not evidently regular. (2) As for the impact strength on large spatial scale, active area＞buffer area＞background area; and from the aspect of sample land scale, the sample lands of floating bridge and stage performances in the active area were under the greatest impact from tourists, while amusement park and tea house were not so strongly affected; the flower viewing2 sample land in buffer area was more affected than the flower viewing3 sample land; the background area was the least affected. (3) Bulk density, organic matter and available nitrogen in sample lands show regular changes in spatial heterogeneity, while the change of available phosphorus and available potassium was not regular enough. The impact of tourism activities on overall spatial heterogeneity of soil showed a homogeneity tendency.

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Changes of diazotrophic communities in response to cropping systems in a Mollisol of Northeast China

PeerJ ◽

10.7717/peerj.9550 ◽

2020 ◽

Vol 8 ◽

pp. e9550

Author(s):

Jiaxun Zou ◽

Qin Yao ◽

Junjie Liu ◽

Yansheng Li ◽

Fuqiang Song ◽

...

Keyword(s):

Relative Abundance ◽

Northeast China ◽

Cropping Systems ◽

Cropping System ◽

Black Soil ◽

Shannon Index ◽

Available Phosphorus ◽

Continuous Cropping ◽

Rotation System ◽

Diazotrophic Community

Nitrogen-fixing microorganisms play important roles in N cycling. However, knowledge related to the changes in the diazotrophic community in response to cropping systems is still rudimentary. In this study, the nifH gene was used to reveal the abundance and community compositions of diazotrophs in the cropping systems of continuous cropping of corn (CC) and soybean (SS) and soybean-corn rotation for growing corn (CSC) and soybean (SCS) in a black soil of Northeast China. The results showed that the abundance of the nifH gene was significantly higher in cropping soybean than in cropping corn under the same cropping system, while remarkably increased in the rotation system under the same crop. The Shannon index in the CC treatment was significantly higher than that in the other treatments, but the OTU number and Chao1 index had no significant change among the four treatments. Bradyrhizobium japonicum was the dominant diazotrophic species, and its relative abundance was at the lowest value in the CC treatment. In contrast, Skermanella sp. had the highest relative abundance in the CC treatment. A PCoA showed that the diazotrophic communities were separated between different cropping systems, and the variation caused by continuous corn cropping was the largest. Among the tested soil properties, the soil available phosphorus was a primary factor in determining diazotrophic community compositions. Overall, the findings of this study highlighted that the diazotrophic communities in black soils are very sensitive to cropping systems.

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Physiological and transcriptomic analyses to reveal underlying phenolic acid action in consecutive monoculture problem of Polygonatum odoratum

BMC Plant Biology ◽

10.1186/s12870-021-03135-x ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Xianzhi Ni ◽

Chenzhong Jin ◽

Aiyu Liu ◽

Yong Chen ◽

Yihong Hu

Keyword(s):

Phenolic Acids ◽

Phenolic Acid ◽

Genomic Analysis ◽

Soil Nutrient ◽

Available Phosphorus ◽

Continuous Cropping ◽

Nutrient Metabolism ◽

Available Nitrogen ◽

Yield And Quality ◽

Chemical Contents

Abstract Background The root rot of fragrant solomonseal (Polygonatum odoratum) has occurred frequently in the traditional P. odoratum cultivating areas in recent years, causing a heavy loss in yield and quality. The phenolic acids in soil, which are the exudates from the P. odoratum root, act as allelochemicals that contribute to the consecutive monoculture problem (CMP) of the medicinal plant. The aim of this study was to get a better understanding of P. odoratum CMP. Results The phenolic acid contents, the nutrient chemical contents, and the enzyme activities related to the soil nutrient metabolism in the first cropping (FC) soil and continuous cropping (CC) soil were determined, and the differentially expressed genes (DEGs) related to the regulation of the phenolic acids in roots were analyzed. The results showed that five low-molecule-weight phenolic acids were detected both in the CC soil and FC soil, but the phenolic acid contents in the CC soil were significantly higher than those in the FC soil except vanillic acid. The contents of the available nitrogen, available phosphorus, and available potassium in the CC soil were significantly decreased, and the activities of urease and sucrase in the CC soil were significantly decreased. The genomic analysis showed that the phenolic acid anabolism in P. odoratum in the CC soil was promoted. These results indicated that the phenolic acids were accumulated in the CC soil, the nutrient condition in the CC soil deteriorated, and the nitrogen metabolism and sugar catabolism of the CC soil were lowered. Meantime, the anabolism of phenolic acids was increased in the CC plant. Conclusions The CC system promoted the phenolic acid anabolism in P. odoratum and made phenolic acids accumulate in the soil.

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Straw mulching and nitrogen application altered ammonia oxidizers communities and improved soil quality in the alkaline purple soil of southwest China

AMB Express ◽

10.1186/s13568-021-01211-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Songhe Chen ◽

Rencai Gao ◽

Xiaoling Xiang ◽

Hongkun Yang ◽

Hongliang Ma ◽

...

Keyword(s):

Soil Quality ◽

Ammonia Oxidation ◽

Management Strategies ◽

Rflp Analysis ◽

Available Phosphorus ◽

Purple Soil ◽

Ammonia Oxidizers ◽

Nitrogen Application ◽

Available Nitrogen ◽

Straw Mulching

AbstractMicrobe-mediated ammonia oxidation is a key process in soil nitrogen cycle. However, the effect of maize straw mulching on the ammonia oxidizers in the alkaline purple soil remains largely unknown. A three-year positioning experiment was designed as follows: straw mulching measures as the main-plot treatment and three kinds of nitrogen application as the sub-plot treatment. We found the contents of soil organic carbon (SOC), total nitrogen (TN), available potassium (AK), available nitrogen (AN), available phosphorus (AP), and NH4+-N were increased after straw mulching and nitrogen application in alkaline purple soil, so did the amoA genes abundance of ammonia-oxidizing archaeal (AOA) and bacterial (AOB). Terminal restriction fragment length polymorphism (T-RFLP) analysis revealed that Thaumarchaeote (448-bp T-RF) was dominated the AOA communities, whereas Nitrosospira sp (111-bp T-RF) dominated the AOB communities. The community compositions of both AOA and AOB were altered by straw mulching and nitrogen application in alkaline purple soil, however, the AOB communities was more responsive than AOA communities to the straw mulching and nitrogen application. Further analysis indicated that SOC and AP were the main factors affecting the abundance and community compositions of AOA and AOB in alkaline purple soil. The present study reported that straw mulching and nitrogen strategies differently shape the soil ammonia oxidizers community structure and abundance, which should be considered when evaluating agricultural management strategies regarding their sustainability and soil quality.

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Effects of crop type on soil microbial properties in the cropland of the Jianghan plain of China

Plant Soil and Environment ◽

10.17221/283/2018-pse ◽

2018 ◽

Vol 64 (No. 9) ◽

pp. 421-426

Author(s):

Li Jun ◽

Liu Lixin ◽

Zhang Chunlei ◽

Chen Chang ◽

Lu Guangyuan ◽

...

Keyword(s):

Triticum Aestivum L ◽

Invertase Activity ◽

Shannon Index ◽

Available Phosphorus ◽

Jianghan Plain ◽

Brassica Napus L ◽

Microbial Properties ◽

Soil Microbial ◽

Soil Available Phosphorus ◽

Soil Microbial Properties

Soil microbial properties are varied by growing different crops, ultimately reflecting the growth and reproduction of crops. In this study, two types of oilseed rape (Brassica napus L. ZS11 and ZY821) and wheat (Triticum aestivum L. ZM9023) were planted in the Jianghan plain of China. Rhizosphere soil samples were collected three months after sowing. Soil physicochemical properties, enzyme activities and microbial diversity were determined. The results showed that soil available phosphorus significantly increased from 25.57 mg/kg (ZM9023) to 33.20 mg/kg (ZS11) and 35.72 mg/kg (ZY821), respectively. Invertase activity of ZS821 (0.86 mg glucose/g) was significantly lower than in ZS11 (1.04 mg glucose/g). Acid phosphatase activity under planting rapes was significantly higher than that under wheat. Urease activities significantly increased from 40.88 mg NH4+-N/g soil/24 h (NFP) to 49.04 mg NH4+-N/g soil/24 h (FNP) and 51.28 mg NH4+-N/g soil/24 h (ZM9023), 51.60 mg NH4+-N/g soil/24 h (ZY821) and 52.28 mg NH4+-N/g soil/24 h (ZS11), respectively. The ACE (abundance based coverage estimator) and Chao1 indexes of bacteria of ZS11 were lower than ZY821, which were similar to ZM9023. Fertilization increased soil bacterial ACE and Chao1 indexes. However, ACE and Chao1, Shannon and Simpson indexes of soil fungi for ZS11 were significantly higher than in ZY821, which were similar to ZM9023 (except for the Shannon index).

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Effect of biofertilizers on soil microbial count, nutrient availability and uptake under november sown onion

Journal of Applied and Natural Science ◽

10.31018/jans.v9i1.1149 ◽

2017 ◽

Vol 9 (1) ◽

pp. 55-59

Author(s):

Dilpreet Talwar ◽

Kulbir Singh ◽

Jagdish Singh

Keyword(s):

Nutrient Availability ◽

Arbuscular Mycorrhizae ◽

N Uptake ◽

Nutrient Status ◽

Recommended Dose ◽

Available Phosphorus ◽

Microbial Count ◽

Available Nitrogen ◽

Soil Microbial ◽

The Impact

Biofertilizers improves the soil microbial content, Soil nutrient status and nutrient uptake by plant. In an experiment, fifteen treatments comprised of various combinations of biofertilizers, organic manures and chemical fertilizers were compared to access the impact of different sources of nutrient on performance of onion. The highest soil organic carbon (0.40%) was observed in the treatments T12 (Farm Yard Manure (FYM) @ 20 t/ha) and T11 (FYM myctes count (29.9 X 104) was recorded in T11 (FYM @ 20 t/ha + Azotobacter + VAM) treatment while highest fungal @ 20 t/ha + Azotobacter + Vesicular-Arbuscular Mycorrhizae (VAM)). Highest bacterial (24.5 X 106) and actino-count (17.5 X 103) was observed in T3 (Azospirillium+ Recommended dose of NPK) treatment. At the time of harvesting, available nitrogen (N), available phosphorus (P) and available potassium (K) were higher in treatment T3 (Azospirillium + Recommended dose of NPK), T9 (Azotobacter+ VAM + Recommended dose of NPK) and T13 (Poultry treatment (162.6 Kg ha-1) as compared to all other treatments except T1 and T9 treatments while P uptake (13.6 Kg ha-Manure @ 5t/ha) treatments respectively than that in other treatments. Azospirillum and Azotobacter application along with recommended dose of N, P and K improved the fertility status of soil. The N uptake was significantly higher in T3 treatments. The present study highlights the need of use of biofertilizers along with organic and inorganic 1) was significantly higher in T9 treatment than that in other treatments except T1, T3, T5 and T7 treatments. The K uptake was significantly higher in T3 treatment (126.9 Kg ha-1) as compare to all other treatments except T1 and T9 manures/fertilizer to enhance the nutrient availability and improve soil health.

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ASSESSMENT OF THE INFLUENCE OF ABIOTIC FACTORS ON THE ENZYMATIVE ACTIVITY OF THE SOIL

ÈKOBIOTEH ◽

10.31163/2618-964x-2021-4-2-128-134 ◽

2021 ◽

Vol 4 (2) ◽

pp. 128-134

Author(s):

E.V. Tovstik ◽

◽

A.S. Olkova ◽

Keyword(s):

Enzymatic Activity ◽

Soil Solution ◽

Ph Value ◽

Abiotic Factors ◽

Inverse Correlation ◽

Invertase Activity ◽

Soil Environment ◽

Factors Affecting ◽

Activity Of Enzymes ◽

Made In

Аn attempt is made in this work to establish correlations between the level of enzymatic activity of the soil and factors of an abiotic nature. It was found that the activity of invertase and urease in the soils of more southern territories is higher than that of northern ones. In soils with a pH value of the soil environment close to neutral reaction, the level of enzymatic activity is higher than in more acidic ones. The most sensitive to soil acidity among the studied urease enzymes. In relation to zinc, an inverse correlation was established between its content in the soil and the level of invertase activity. According to the degree of resistance to salinity, the enzymes are arranged in the following order: catalase> invertase> urease. An increase in the mineralization of the soil solution leads to an increase in the activity of urease. Of the studied enzymes, the most labile are representatives of the class of hydrolases (invertase and urease), less labile are oxidoreductases (catalase). Thus, when diagnosing the state of the soil by the level of enzymatic activity, it is necessary to take into account the main abiotic factors affecting the activity of enzymes: the average annual air temperature; pH and mineralization of the soil solution; the content of substances that inhibit microorganisms and block exozymes.

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