scholarly journals Valorization of Cyprinus Carpio skin for biocompatible collagen hydrolysates with potential application in foods, cosmetics and pharmaceuticals

2021 ◽  
Author(s):  
Elena Danila ◽  
Raluca Stan ◽  
Mădălina ALBU KAYA ◽  
Georgeta VOICU ◽  
Maria Minodora MARIN ◽  
...  
Keyword(s):  
Cyprinus Carpio ◽  
Ph Value ◽  
Human Keratinocytes ◽  
Cd Spectroscopy ◽  
Random Coil ◽  
Collagen Hydrolysate ◽  
Bovine Collagen ◽  
Fish Collagen ◽  
Collagen Hydrolysates ◽  
Hydrolysis Of

Abstract Fish collagen is the safest source of collagen at present, the extraction being performed on secondary sources as skin, bones, scales or fins resulted after fish processing. The aim of the present study was to obtain biocompatible collagen hydrolysates from waste Cyprinus carpio skin, the main aquaculture species in Romania using an inexpensive and “green” neutral hydrolysis process. Neutral hydrolysis of pretreated fish skins performed for 6 hours at a temperature of 135°C and a pressure of 315 kPa produced collagen hydrolysates in 24.6-35.5% yields depending on the adopted pretreatment procedure. The extensive characterization of hydrolysate samples revealed a high purity degree (98% protein content, undetected ash content, pH value in the range 6-7), also confirmed by the absence of undesired aggregates in the characteristic fibril structure as determined by electronic microscopy. A specific collagen hydrolysate random coil structure and the absence of triple helix was determined by FTIR analysis and sustained by CD spectroscopy and X-ray diffraction. The biocompatibility assessment for the obtained fish collagen hydrolysates revealed no cytotoxic effect on Human keratinocytes, with an 80% cell viability, superior as compared to conventional bovine collagen hydrolysate.Neutral hydrolysis of waste Cyprinus carpio skin yielded collagen hydrolysates with determined characteristics and biocompatibility superior to bovine collagen, suitable for application in foods, cosmetics and pharmaceutical industry.

scholarly journals Bioactivity and Tablet Formulation from Spirulina and Collagen Hydrolysate of Tilapia (Oreochromis niloticus) Skin

2019 ◽  
Vol 22 (3) ◽  
pp. 453-463
Author(s):  
Mirza Gulam Ahmad ◽  
Iriani Setyaningsih ◽  
Wini Trilaksani
Keyword(s):  
Antioxidant Activity ◽  
Magnesium Stearate ◽  
Tablet Formulation ◽  
Collagen Hydrolysate ◽  
Second Stage ◽  
Skin Collagen ◽  
Third Stage ◽  
Collagen Hydrolysates ◽  
Hydrolysis Of

Spirulina is a microalga known as a superfood which contains high protein and is used in cosmetics, foods, and supplements industry. Collagen hydrolysate is the result of hydrolysis of collagen by protease. This study was aimed to determine the antioxidant activity and inhibition of tyrosynase from Spirulina, collagen hydrolysate and tablet formulated by combination of Spirulina and collagen hydrolysate. This research was conducted in three stages. The first stage was the extraction and hydrolysis of tilapia skin collagen, while the second stage was culturing and harvesting of Spirulina, and the third stage was determination of tablet formulation based on Spirulina and collagen hydrolysate followed by analysis of the antioxidant activity and inhibition of tyrosinase by selected tablet. Three formula with different ratio between Spirulina and collagen hydrolysates namely formula A (25%:53.75%), formula B (28.125%:50.625%), formula C (31.25%:47.5%) and additive such as vitamin C (12.5%), talk (1.25%), magnesium stearate (0.625%), aerosil (1.875%), avicel (5%) were developed. The result showed that formula A was the selected formula meeting the requirements of Ministry of Health based on the physical characteristics. The antioxidant activity of tablet A was 843 ppm and the tyrosinase inhibition was 163.56 ppm.

scholarly journals Problems of efficient processing and use of collagen-containing materials

2015 ◽  
Vol 87 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Maksym Koliada ◽  
Vikroriia Plavan
Keyword(s):  
Feed Additives ◽  
Fish Processing ◽  
Processing Waste ◽  
Collagen Hydrolysate ◽  
Hydrolysis Method ◽  
Fish Collagen ◽  
Other Substances ◽  
Efficient Processing ◽  
Collagen Hydrolysates ◽  
Time And Energy

AbstractCollagen is an important biopolymer in numerous applications due to its special characteristics, such as biodegradability and weak antigenecity. Interest recently, has grown in fish collagen. This stems from the fact, that the use of animal collagen is unsafe due to the effects from cattle rabies disease. Furthermore, fish collagen is 96 % identical to human protein. Modern fish production is accompanied by the formation of a large number of protein-containing wastes. Depending on the degree of fish processing waste hydrolysis we obtain different products, such as feed additives or growth accelerators. Available technologies are unacceptable, because they require the consumption of large amounts of time and energy. The enzymatic method of obtaining collagen hydrolysates is the most suitable because it can be implemented under milder conditions and it prevents the destruction of amino acids, carbohydrates and other substances contained in the waste. Application of the alkaline enzymatic hydrolysis method with hydrogen peroxide pretreatment at elevated temperature provides a collagen hydrolysate, which is characterized by a high content of total nitrogen; collagen is amorphous, has fully homogeneous structure and has a balanced amino acid composition.

scholarly journals The effect of drying temperature on the properties of gelatin from carps (Cyprinus carpio) skin

2019 ◽  
Vol 37 (No. 4) ◽  
pp. 246-251 ◽  
Author(s):  
Joanna Tkaczewska ◽  
Maciej Wielgosz ◽  
Piotr Kulawik ◽  
Marzena Zajac
Keyword(s):  
Cyprinus Carpio ◽  
Freeze Drying ◽  
Treatment Method ◽  
Thermal Hydrolysis ◽  
Gel Properties ◽  
Drying Temperature ◽  
Freeze Dried ◽  
Different Temperatures ◽  
Pre Treatment ◽  
Hydrolysis Of

The influence of drying temperature on the characteristics and gel properties of gelatine from Cyprinus carpio L. skin was studied. Gelatine was extracted from the carp skin using NaOH and ethanol pre-treatment method, extracted in water in 45°C and then dried in 4 different temperatures: 50, 70, 80°C and freeze-dried. The  electrophoresis and functional properties of gelatines were investigated. Freeze drying allowed to obtain a high gelling force, and all other methods did not give satisfactory results. The proteins in gelatines dried at higher temperatures separated by electrophoresis gave severely blurred bands. It may be explained by thermal hydrolysis of collagen fibrils. Freeze drying is the only effective method for drying this product, which can be used in industry.

The Formation of Plasma Acid at Atmospheric Pressure and its Application in Hydrolysis of Microcrystalline Cellulose

2013 ◽  
Vol 750-752 ◽  
pp. 1626-1629
Author(s):  
Bo Yuan ◽  
Ying Wang ◽  
Ying Chao Ji ◽  
Qiu Hong Wang
Keyword(s):  
Microcrystalline Cellulose ◽  
Atmospheric Pressure ◽  
High Performance ◽  
Barrier Discharge ◽  
Ph Value ◽  
Reducing Sugar ◽  
Dielectric Barrier ◽  
Integrated Optical ◽  
Hydrolysis Of ◽  
Single Factor Experiment

In this paper, plasma acid was obtained by treating distilled water with dielectric barrier discharge at atmospheric pressure in order to hydrolyze cellulose. The acidity of plasma acid was studied through a single factor experiment. A plasma acid with pH value of 1.42 was obtained and used to hydrolyze microcrystalline cellulose at 80°C for 60min. Under this condition, the integrated optical density (IOD) of the hydrolysis sample was 0.589. Based on standard glucose curve, the total reducing sugar (TRS) was calculated to be 53.75mg and the TRS yield was 53.75%. The filtrate was evaporated to get the solid hydrolysis sample to be analyzed by High-performance liquid chromatography (HPLC). The results showed that the sample mainly consisted of glucose, which proved that microcrystalline cellulose could be hydrolyzed by plasma acid. Therefore, it could be concluded that it was an environmentally friendly and economical method to hydrolyze the microcrystalline cellulose by plasma acid.

scholarly journals NH<sub>3</sub>-promoted hydrolysis of NO<sub>2</sub> induces explosive growth in HONO

2018 ◽  
Author(s):  
Wanyun Xu ◽  
Ye Kuang ◽  
Chunsheng Zhao ◽  
Jiangchuan Tao ◽  
Gang Zhao ◽  
...  
Keyword(s):  
North China Plain ◽  
North China ◽  
Ph Value ◽  
Primary Source ◽  
Field Measurements ◽  
Oh Radicals ◽  
The North ◽  
The North China Plain ◽  
First Time ◽  
Hydrolysis Of

Abstract. The study of atmospheric nitrous acid (HONO), which is the primary source of OH radicals, is crucial to atmospheric photochemistry and heterogeneous chemical processes. The heterogeneous NO2 chemistry under haze conditions was pointed out to be one of the missing sources of HONO on the North China Plain, producing sulfate and nitrate in the process. However, controversy exists between various proposed mechanisms, mainly debating on whether SO2 directly takes part in the HONO production process and what roles NH3 and the pH value play in it. In this paper, never before seen explosive HONO production (maximum rate: 16 ppb/hour) was reported and evidence was found for the first time in field measurements during fog episodes (usually with pH > 5) and haze episodes under high relative humidity (usually with pH 

scholarly journals Biochemical and pharmacological characterization of fish collagen hydrolysates

2014 ◽  
Vol 22 ◽  
pp. S325
Author(s):  
S. Schadow ◽  
H-C. Siebert ◽  
G. Lochnit ◽  
M. Rickert ◽  
J. Steinmeyer
Keyword(s):  
Pharmacological Characterization ◽  
Fish Collagen ◽  
Collagen Hydrolysates

scholarly journals Coating of Seeds with Collagen Hydrolysates from Leather Waste

2019 ◽  
Vol 27 (4(136)) ◽  
pp. 59-64 ◽  
Author(s):  
Katarzyna Ławińska ◽  
Magdalena Lasoń-Rydel ◽  
Dorota Gendaszewska ◽  
Edyta Grzesiak ◽  
Katarzyna Sieczyńska ◽  
...  
Keyword(s):  
Polyvinyl Alcohol ◽  
Growth And Yield ◽  
Collagen Hydrolysate ◽  
Drought Conditions ◽  
Different Types ◽  
Mineral Additive ◽  
Encapsulation Process ◽  
The Subject ◽  
Collagen Hydrolysates ◽  
The Impact

The subject of this paper is improvement in the growth and yield of three different types of legumes and rape in drought conditions by coating seeds with hydrolysed collagen from tanning waste. In addition, the impact of various additives in the seed shell on the growth of the plant was investigated. The encapsulation process of seeds was conducted on a disc granulator. A centrally placed seed was first coated with a layer of fungicides. The next layer was collagen hydrolysate, collagen hydrolysate with latex or a solution of yellow dextrin and polyvinyl alcohol. The outer layer was a mineral additive e.g. dolomite or kaolin. After the end of the encapsulation process on the disk granulator, all of the seeds tested were sown into soils. Seeds without coating were also sown as control seeds. Seedlings were maintained for 29 days with cultivation without irrigation. The length of the seedlings was analysed for all of the seeds sown. Higher seedling growth values were obtained for seeds coated with collagen hydrolysate in comparison with control seeds (without coating). The use of collagen hydrolysate gave slightly better results than in the case of a solution of dextrin with polyvinyl alcohol.

Optimization of olive pomace enzymatic hydrolysis for fermentable sugar production

2016 ◽  
Vol 46 (6) ◽  
pp. 778-790 ◽  
Author(s):  
Ghassan Abo Chameh ◽  
Fadi Kheder ◽  
Francois Karabet
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ph Value ◽  
Maximum Yield ◽  
Olive Pomace ◽  
Mixing Rate ◽  
Content Type ◽  
Hydrolysis Yield ◽  
Pretreatment Time ◽  
Hydrolysis Conditions ◽  
Hydrolysis Of

Purpose The purpose of this paper was to find out the appropriate enzymatic hydrolysis conditions of alkali pretreated olive pomace (OP) which enable maximum yield of reducing sugar. Design/methodology/approach The commercial enzymatic preparation (Viscozyme® L) was used for the hydrolysis of OP. The effects of pretreatment, time, temperature, pH, enzyme quantity and substrate loading on the hydrolysis yield were investigated. Findings This study showed that enzymatic hydrolysis of OP using Viscozyme® L can be successfully performed at 50°C. Alkaline pretreatment step of OP prior the enzymatic hydrolysis was indispensable. The hydrolysis yield of alkaline pretreated OP was 2.6 times higher than the hydrolysis yield of untreated OP. Highest hydrolysis yield (33.5 ± 1.5 per cent) was achieved after 24 h using 1 per cent (w/v) OP load in the presence of 100 μl Viscozyme® L at 50°C and pH 5.5 with mixing rate of 100 rpm (p = 0.05). Originality/value Reaction time, temperature, pH value and enzyme quantity were found to have a significant effect on enzymatic hydrolysis yield of alkali pretreated of OP. Although high-solid loadings of OP lowered the hydrolysis yield, it produced higher concentration of reducing sugars, which may render the OP conversion process more economically feasible.

scholarly journals pH- and concentration-dependent supramolecular self-assembly of a naturally occurring octapeptide

2020 ◽  
Vol 16 ◽  
pp. 2017-2025
Author(s):  
Goutam Ghosh ◽  
Gustavo Fernández
Keyword(s):  
Self Assembly ◽  
Ph Value ◽  
Secondary Structures ◽  
Random Coil ◽  
Conformational Transformation ◽  
Stimuli Responsive ◽  
Responsive Materials ◽  
Force Microscopy ◽  
Assembly Behavior ◽  
Novel Drug

Peptide-based biopolymers represent highly promising biocompatible materials with multiple applications, such as tailored drug delivery, tissue engineering and regeneration, and as stimuli-responsive materials. Herein, we report the pH- and concentration-dependent self-assembly and conformational transformation of the newly synthesized octapeptide PEP-1. At pH 7.4, PEP-1 forms β-sheet-rich secondary structures into fractal-like morphologies, as verified by circular dichroism (CD), Fourier-transform infrared (FTIR) spectroscopy, thioflavin T (ThT) fluorescence spectroscopy assay, and atomic force microscopy (AFM). Upon changing the pH value (using pH 5.5 and 13.0), PEP-1 forms different types of secondary structures and resulting morphologies due to electrostatic repulsion between charged amino acids. PEP-1 can also form helical or random-coil secondary structures at a relatively low concentration. The obtained pH-sensitive self-assembly behavior of the target octapeptide is expected to contribute to the development of novel drug nanocarrier assemblies.

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