HPV prevalence and genotype distribution among women from Hengyang district of Hunan province, China

Abstract Background Human papillomavirus (HPV) infection can cause a variety of epithelial lesions, including even various malignant cancers, such as cervical cancer, vulvar cancer and etc. Most cervical cancers were closely associated with HPV infections. Therefore, understanding the ecological diversity of HPV prevalence and genotype distribution among various populations in different geographical regions was essential for optimizing HPV vaccination and maximizing vaccination effects. Aim This study was designed to investigate HPV infection prevalence and genotype distribution among women from Hengyang district of Hunan province in China.Methods A total of 12053 patient data from the three-level hospitals in Hengyang city between July 2016 and July 2017 were retrospectively analyzed. The HPV positive was identified by Polymerase chain reaction (PCR). The HPV PCR-Flow fluorescence assay was used for HPV genotyping. The Chi-square test was performed to compare the differences among HPV infection rates or HR-HPV ratio-in-positives.Results In this study, the HPV prevalence was 10.16% overall, and the multiple-type infection rate was 1.83%. The HR-HPV infection rate was 8.52%. The top six HPV genotypes were as follows in a descending order: HPV16, HPV58, HPV52, HPV39, HPV51, and HPV53. The patients who were above 60 years and below 21 years old were the most and second HPV prevalent group, respectively, the corresponding HR-HPV infection rates were the most and the third, respectively. The infection rates of HPV and HR-HPV among outpatients were lower than those among hospitalized-patients, respectively. Among the hospitalized-patients, the infection rates of HPV and HR-HPV among 50~60 years group were both the most, following by those among above 60 years group. The HR-HPV ratio-in-positive among HPV-positive patients with histopathologic examination was higher than that among those patients without. Among 52 HPV-positive patients with cervical squamous carcinoma, the ratio-in-positive of HPV16 was 61.54%.Conclusions This study demonstrated that the HPV prevalence varied with the age among women from Hengyang district of Hunan province in China and showed their HPV genotype distribution that HPV16, HPV58, HPV52, HPV39, HPV51 and HPV53 infection were more popular in this region, which could provide the experimental basis for Chinese public health measures on cervical cancer prevention.

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Human papillomavirus genotype distribution in cervical intraepithelial neoplasia 2/3 and invasive cervical cancer among Hakka women in southern China

10.21203/rs.2.9816/v1 ◽

2019 ◽

Author(s):

Xiaodong Gu ◽

Ruiqiang Weng ◽

Jing Liu ◽

Sudong Liu

Keyword(s):

Cervical Cancer ◽

Cervical Intraepithelial Neoplasia ◽

Southern China ◽

Invasive Cervical Cancer ◽

Intraepithelial Neoplasia ◽

Hpv Infection ◽

Histological Diagnosis ◽

Infection Prevalence ◽

Genotype Distribution ◽

Hpv Genotypes

Abstract Background: To investigate HPV genotype distribution in cervical intraepithelial neoplasia 2/3 (CIN2/3) and invasive cervical cancer (ICC) among Hakka women in southern China. Methods: Results of HPV genotypes from women with histological diagnosis of CIN2/3 and ICC were collected from January, 2017 to December, 2018. HPV genotypes were analyzed by flow cytometry method. Association of HPV infection and lesions severity was estimated using prevalence ratio (PR). Results: Overall, 1,408 Hakka women with histological diagnosis of CIN2/3 and ICC were enrolled in this study. HPV infection prevalence was 92.92% in CIN2, 95.77% in CIN3 and 95.88% in ICC. Most frequent genotypes for CIN2 were HPV52 (31.42%), HPV16 (22.12%) and HPV58 (22.12%); for CIN3 were HPV16 (41.90%), HPV52 (20.77%) and HPV58 (18.31%); and for ICC were HPV16 (49.67%), HPV18 (11.25%) and HPV52 (9.80%). PR of HPV16 and HPV33 were significantly higher in CIN3 compared with CIN2 (PR = 2.372, 95%CI = 1.598-3.524; PR = 2.577, 95%CI = 1.250-5.310; respectively). HPV16 and HPV18 prevalence were significantly increasing in SCC compared with CIN3 (PR = 2.517, 95%CI = 1.095-5.786; PR = 2.473, 95%CI = 1.840-3.324; respectively). Most HPV infections were found in women aged 40 – 49 years in CIN2/3 and women aged 50 - 59 years in ICC. Conclusions: This is the first study of genotypes and age specific distribution of HPV infection among Hakka women with CIN2/3 and ICC in southern China. Our results provide available information for HPV vaccine development in China.

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Prevalence and distribution of human papillomavirus genotypes among women in northern Henan Province of China

10.21203/rs.3.rs-889505/v1 ◽

2021 ◽

Author(s):

Xiangpeng Wang ◽

Yuan Song ◽

Xiaofei Wei ◽

Guanyu Wang ◽

Ruili Sun ◽

...

Keyword(s):

Human Papillomavirus ◽

Age Groups ◽

Hpv Infection ◽

Cervical Cancer Prevention ◽

Henan Province ◽

Infection Prevalence ◽

Genotype Distribution ◽

Hpv Dna ◽

Hpv Prevalence ◽

Specific Hybridization

Abstract Background Human papillomavirus (HPV) infection can cause cervical and other cancers including cancer of vulva, vagina, penis, anus, or oropharynx. However, data concerning the prevalence and genotype distribution of HPV among women are limited in northern Henan Province of China. This study aimed to make an investigation on the current prevalence and genotype distribution of HPV among women and provided comprehensive data to guide HPV-based cervical cancer prevention in northern Henan Province. Methods A total of 15616 women aged 16 to 81 years who attended the department of gynecology of Xinxiang central hospital between January 2018 and December 2019 were enrolled in this study. HPV DNA was detected by PCR method followed by HPV type-specific hybridization. The overall prevalence, age-specific prevalence and genotype distribution of HPV were investigated. Results The overall HPV prevalence was 19.7% among women in northern Henan Province. Single, double and multiple HPV infections accounted for 13.7%, 4.3% and 1.8% of the total cases, respectively. HPV prevalence was 41.8% among women aged 16 to 19 years, 21.1% among women aged 20 to 29 years, 18.1% among women aged 30 to 39 years, 17.7% among women aged 40 to 49 years, 20.8% among women aged 50 to 59 years, 22.9% among women more than 60 years. HPV infection rates differed significantly across different age groups. Most infections were caused by high-risk HPV (HR-HPV) and single genotype HPV infection was the most common pattern. The most common HR-HPV genotype was HPV16, followed by HPV52, HPV58, HPV53 and HPV39. The most common low-risk HPV (LR-HPV) genotype was HPV6, followed by HPV61, HPV81, HPV54 and HPV11. Conclusions HPV infection is common among women in northern Henan Province. The highest infection prevalence was found in women less than 20 years old. The 9-valent HPV vaccine for routine vaccination is strongly recommended in northern Henan Province.

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Prevalence of HPV infection among 157,038 Chinese females in Hunan Province, central-south China: Research article

10.21203/rs.2.11877/v1 ◽

2019 ◽

Author(s):

Chen Wang ◽

Songshu Xiao ◽

Sili He ◽

Qiong Pan ◽

Jie Zeng ◽

...

Keyword(s):

Infection Rate ◽

South China ◽

Hpv Infection ◽

Hunan Province ◽

Genotype Distribution ◽

The Third ◽

Research Article ◽

Hpv Types ◽

The Common ◽

Central South

Abstract Background: In the present study, we aimed to investigate the human papillomavirus (HPV) infection rate in females of Hunan Province, China, as well as the common HPV genotype distribution. Moreover, we also explored the differences in HPV infections among females of different ages. Methods: Clinical data were collected from 157,038 females who had tested HPV infection in the Third Xiangya Hospital of Central South University from November, 2010 to May, 2017. Results: The overall HPV infection rate was 19.91%.The most commonly detected genotypes were HPV52 (4.62%), HPV16 (3.52%), HPV 58(3.12%), HPVCP8304 (2.91%), and HPV53 (2.06%). The highest infection rate was found in females under the age of 20 (30.33%), the second highest infection rate was found in females over the age of 60 (24.72%), and females aged 30-39 showed the lowest HPV infection rate (18.11%). In addition, 71.32% of the infections were single HPV infections. Among the multiple HPV infections, HPV16/HPV6 co-infection was the most commonly detected combination (0.52%). Conclusions: In this study, we examined the epidemiology of HPV and the prevalence of the common HPV infection in Hunan Province, central-south China. We have revealed the prevalence and distribution of the different HPV types. Our study showed that females under the age of 20 and over the age of 60 were at higher risk of HPV infection than females of other ages. Moreover, our region should make extra efforts to the prevention and treatment of HPV52, HPV16 and HPV58 infections.

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Human papillomavirus and other risk factors in Lithuanian cervical carcinoma patients

Acta medica Lituanica ◽

10.15388/amed.2010.21683 ◽

2010 ◽

Vol 17 (3-4) ◽

pp. 106-115

Author(s):

Živilė GUDLEVIČIENĖ ◽

Alė SMILGEVIČIŪTĖ-IVSHIN ◽

Aurelija VAITKUVIENĖ ◽

Agnė ŠEPETIENĖ ◽

Janina DIDŽIAPETRIENĖ

Keyword(s):

Risk Factors ◽

Cervical Cancer ◽

Cancer Risk ◽

Hpv Infection ◽

Cancer Development ◽

Cancer Risk Factors ◽

Cervical Cancer Risk ◽

Hpv Prevalence ◽

Geographical Regions ◽

Cervical Cancer Development

Background. Cervical cancer is the second biggest cause of female cancer mortality worldwide and the most common cancer in women in Lithuania. The incidence of cervical cancer is strongly associated with HPV prevalence. However, not only HPV infection plays a crucial role in cervical cancer development; other risk factors which vary in different populations and geographical regions as well as HPV prevalence are important. The aim of this study was to detect the HPV, its type’s prevalence and other cervical cancer risk factors for Lithuanian women. Materials and methods. 191 women with primary diagnosed invasive cervical cancer (cases group) and 397 control women were invited to participate in the study. All women were interviewed and samples for HPV testing were taken. Results. In the cases group, 92.7% of women and in the control group 26.7% were infected by HPV (p < 0.0001). HPV 16 was the most common type in both groups. HPV infection increases the risk of cervical cancer 75 times (OR = 75.39; 95% CI 33.61–192.98). Women with lower education, workers, those who started sexual intercourses before 20 years of age, at older age of the first menstrual period, 3–5 or more childbirths, smokers and with a long ago or never performed Pap test are at a significantly higher risk of cervical cancer development. Other non-HPV risk factors were not associated with cervical cancer risk. Conclusions. Data of our study show a high prevalence of HPV in Lithuanian population. It may have an impact on the biggest cervical cancer incidence. The other risk factors are similar as in other lower economic resource countries. Keywords: HPV, cervical cancer, risk factors

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HPV16 E6 gene polymorphisms and the functions of the mutation site in cervical cancer among Uygur and Han women in Xinjiang

10.21203/rs.3.rs-16235/v1 ◽

2020 ◽

Author(s):

Huizhen Xin ◽

Zhenzhen Pan ◽

Xiangyi Zhe ◽

Dongmei Li ◽

Chunhe Zhang ◽

...

Keyword(s):

Cervical Cancer ◽

Infection Rate ◽

Hpv Infection ◽

Eukaryotic Expression ◽

Migration And Invasion ◽

Genotype Distribution ◽

Mutation Site ◽

Hpv16 E6 ◽

E6 Gene ◽

C33a Cell

Abstract Objective : This study aimed to: 1) investigate the status and genotype distribution of human papillomavirus (HPV) in infected Uygur and Han women in Xinjiang; 2) elucidate the variation of the HPV16 E6 gene sequence in the cervix of Uygur and Han women in Xinjiang; and 3) analyze the HPV16 E6 gene polymorphism site and relationship with the development of cervical cancer. Methods : A total of 2879 samples of cervical mucus from the exfoliated cells of Uygur and Han women were collected for an epidemiological analysis of HPV. Genomic DNA was extracted from the cervical HPV16-positive tissues of 110 Uygur and Han women, and E6 was amplified by PCR and sequenced. The HPV16 E6 sequence was analyzed using the European standard as the prototype, and an evolutionary tree analysis was performed. HPV16 E6-295T/350T-GV230, HPV16 E6-295G/350G-GV230, and HPV16 E6-295T/350G-GV230 were stably transfected into human cervical cancer C33A cells. HPV16 E6 protein expression was confirmed using a direct immunofluorescence assay. CCK8 and clonogenic assays were used to analyze C33A cell proliferation. Both a transwell and cell scratch assay were used to study C33A cell migration and invasion. C33A cell apoptosis was analyzed using FACS experiments. SPSS17.0 statistical software was used for statistical data processing. P < 0.05 was considered statistically significant. Results : The total HPV infection rate was 26.390% (760/2879), whereas the Uygur infection rate was 22.87% (196/857) and the Han infection rate was 27.89% (564/2022) (P < 0.05). HPV16, HPV 52, and HPV 53 were associated with higher detection rates in Uygur, whereas HPV16, HPV52, and HPV58 exhibited a higher detection rate in Han. HPV-infected women from Uygur and Han commonly exhibited a single infection. A total of 14 mutation sites were identified in the HPV16 E6 gene by sequencing 110 HPV16-positive samples, including eight missense and six synonymous mutations. Among these, 65 cases of E6 genes were mutated at nucleotide 350 (T350G) with the corresponding amino acids changing from leucine to valine (L83V) and a mutation rate of 59.09%. Moreover, there were seven cases of an E6 gene mutation at nucleotide 295 (T295G) with corresponding amino acid changes from aspartic to glutamic (D64E) and a mutation rate of 6.36%. It is important to note that these seven cases of HPV16 E6 T295G mutations were accompanied by the E6 T350G mutation. Phylogenetic analysis showed that there were HPV16 European (Ep), European variant (E), and three Asian (As) types in Uygur and Han women. No African (Af) and Asian American (AA) types were observed. When HPV16 E6 295T/350T, 295G/350G, and 295T/350G GV230 eukaryotic expression vector(s) were stably transfected into cervical cancer C33A cells, they were found to promote cellular proliferation, migration, invasion, and inhibit apoptosis. The 295T/350G-GV230 had the strongest effect on C33A cells and 295G/350G-GV230 was significantly stronger than 295T/350T-GV230 (P < 0.05). Conclusions : The positive HPV infection rates differed between the Uygur and Han groups in Xinjiang, and the genotype distribution of HPV infection was different. Between the Uygur and Han women in Xinjiang, the main types of HPV16 infection were European (E) and Asian (As). After stably transfecting C33A cells with a eukaryotic expression vector for different polymorphism sites (295T/350T, 295G/350G, and 295T/350G), the 295T/350G mutation site promoted the proliferation，migration, and invasion of C33A cells to a greater extent than 295G/350G; however, 295G/350G had a stronger effect than 295T/350T.

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Prevalence of human papillomavirus by geographical regions, sexual orientation and HIV status in China: a systematic review and meta-analysis

Sexually Transmitted Infections ◽

10.1136/sextrans-2017-053412 ◽

2018 ◽

Vol 94 (6) ◽

pp. 434-442 ◽

Cited By ~ 13

Author(s):

Xiaomeng Ma ◽

Qian Wang ◽

Jason J Ong ◽

Christopher K Fairley ◽

Shu Su ◽

...

Keyword(s):

Systematic Review ◽

Cervical Cancer ◽

Human Papillomavirus ◽

Meta Analysis ◽

Hpv Vaccination ◽

Hpv Infection ◽

Central China ◽

Hiv Positive ◽

Heterosexual Men ◽

Hpv Prevalence

ObjectiveHuman papillomavirus (HPV) infection causes multiple cancers in both women and men. In China, both HPV vaccination and cervical cancer screening coverages are low. We aim to investigate the temporal and geographical trends of HPV DNA prevalence in heterosexual men, women, men who have sex with men (MSM) and people living with HIV (PLHIV) in China.MethodsWe conducted a systematic review, collecting publications in PubMed, Web of Science, China National Knowledge Infrastructure (CNKI) and Wanfang Data from January 2000 to May 2017. A total of 247 studies were selected for this meta-analysis to estimate pooled HPV prevalence, incidence of cervical cancer and risk of infection for subgroups. Meta-regression was applied to identify contributing factors to prevalence heterogeneities.ResultsThe national HPV prevalence was 15.6% (95% CI (14.4% to 16.9%)) in women with normal cervical cytology, and Central China had the highest prevalence (20.5% (15.2% to 25.8%)). HPV prevalence in heterosexual men (14.5% (11.3% to 17.7%)) was comparable with that of women (OR=1.09 (0.98 to 1.17)), but HPV prevalence in MSM (59.9% (52.2% to 67.6%)) was significantly higher than that in heterosexual men (OR=8.81 (8.01 to 9.69)). HIV-positive women (45.0% (38.4% to 51.6%)) and HIV-positive MSM (87.5% (82.3% to 90.9%)) had 4.67 (3.61 to 6.03) and 6.46 (5.20 to 8.02) times higher risk of HPV infection than their HIV negative counterparts.ConclusionHPV infection is prevalent in China, particularly in Central China, in comparison with the global level and neighbouring countries. Targeted HPV vaccination for women, MSM and PLHIV and scale-up of cervical screening for women are priorities in curbing the HPV epidemic in China.

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Human papillomavirus infection rate, distribution characteristics, and risk of age in pre- and postmenopausal women

10.21203/rs.3.rs-131821/v1 ◽

2020 ◽

Author(s):

Yan Shen ◽

Jing Xia ◽

Hui hui Li ◽

Yang Xu ◽

San ping Xu

Keyword(s):

Cervical Cancer ◽

High Risk ◽

Postmenopausal Women ◽

Infection Rate ◽

Genital Tract ◽

Hpv Infection ◽

Distribution Characteristics ◽

Lower Genital Tract ◽

Significant Difference ◽

High Risk Hpv

Abstract BackgroundThe incidence rate of cervical cancer is increasing yearly. The persistent infection of high-risk Human Papillomavirus (HPV) is the main factor leading to cervical cancer. HPV infection is double peak type. This study aimed at analyzing the HPV distribution characteristics, infection rate, and risk of age in pre- and postmenopausal women. So as to provide reference for the prevention of HPV infection and cervical cancer screening strategy.MethodsA retrospective analysis of 4614 women who underwent cervical cytology, and HPV examination from January 2018 to October 2019 at the healthcare department of Wuhan Union Hospital was done. We explored the characteristics and distribution of HPV infections around the menopause, then comparing the infection rate of HPV in postmenopause and over 65 years old, in order to analyze the influence of different ages on HPV infection.ResultsGenerally, the HPV infection rate was 13.10% (539 / 4115), whereby the high-risk subtype constituted 73.84% (398 / 539) of all positive cases. On the other hand, the HPV39 infection was more common in postmenopausal women; however, there was no significant difference in the distribution of the other types in the pre- and postmenopausal women (Insert p value). The first four subtypes were 52 / 53 / 58 / 16, respectively. The infection rate of HPV in patients with lower genital tract inflammation was significantly higher, P = 0.000, 95% CI: 1.911 (1.416, 2.580) compared with those without lower genital tract inflammation. The results further showed that there was no significant difference between pre- and postmenopausal women in terms of HPV infection rate, but more susceptible to high-risk HPV infection after the age of 65 (P = 0.041). Except for 40 years old to menopause, the infection rate of high-risk HPV in this age group was different from that in postmenopause(P = 0.023,0.729(0.555,0.957)), other age groups had no significant effect on high-risk HPV infection.ConclusionsIt was concluded that whether menopause has nothing to do with HPV infection. Moreover, the infection rate of high-risk HPV increases after 65 years of age; hence the cutoff screening age should be appropriately prolonged.Trial registration: Retrospectively registered.

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Positivity and prevalence of human papillomavirus among a large population of Hakka women in southern China

10.21203/rs.2.9332/v1 ◽

2019 ◽

Author(s):

Sudong Liu ◽

Xiaodong Gu ◽

Ruiqiang Weng ◽

Jing Liu ◽

Zhixiong Zhong

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Vaccine Development ◽

Screening Program ◽

Southern China ◽

Large Population ◽

Hpv Infection ◽

Infection Prevalence ◽

Specific Distribution ◽

Hpv Genotypes

Abstract Background Human papillomavirus (HPV) infections are known to be the main cause of cervical cancer. Thus, detecting HPV genotypes is of great significance for cervical cancer screening. To formulate strategies for prevention of cervical cancer, we studied the HPV infection prevalence and age-specific distribution of female in Hakka area of southern China. Method From January, 2016 to July, 2018, 36,871 women from gynecological or reproductive center of Meizhou People’s Hospital (Huangtang Hospital), Meizhou Hospital Affiliated to Sun Yat-sen University were recruited in this study. HPV genotypes was detected by flow cytometry method. Results HPV infection was observed in 18.34% of the participants, and approximately 79.98% of them were infected with high-risk HPV. The 5 most prevalent genotypes were HPV 52 (18.18%), HPV 16 (16.06%), HPV 58 (11.37%), HPV 53 (8.82%) and HPV 39 (6.42%). The most intensive HPV infections were observed in women aged 40-49 and women aged 30-59 added up to 79.62% of positive patients. Conclusion The high HPV infection rate in this Hakka area highlighted the necessity of screening program for HPV infection and cervical cancer among women. The findings of HPV genotypes and the age specific distribution of HPV infection in this area will contribute to the future vaccine development.

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Distribution and Prevalence of High-Risk Human Papillomavirus Genotypes in Cervical Specimens

Flora the Journal of Infectious Diseases and Clinical Microbiology ◽

10.5578/flora.68958 ◽

2020 ◽

Vol 25 (3) ◽

pp. 325-331

Author(s):

Erkan Özmen ◽

Ülkü Altoparlak ◽

Muhammet Hamidullah Uyanık ◽

Abdulkadir Gülen

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Age Groups ◽

Hpv Infection ◽

High Rate ◽

Genotype Distribution ◽

Hpv Dna ◽

Hpv Test ◽

Significant Difference ◽

Hpv Types

Introduction: Human papillomavirus (HPV) is frequently a sexually transmitted virus and can cause cervical cancer in women. Cervical cancer is the second most common type of cancer among the developing countries. In this study, cervical HPV DNA positivity and genotype distributions were investigated in female patients living in our region and the results were compared with different studies. Materials and Methods: Between 1 July, 2017 and 1 March, 2019, 433 cervical swabs were sent to Ataturk University, Medical Faculty Hospital, Medical Microbiology Laboratory due to suspicion of HPV. Swab samples were evaluated for HPV virus using molecular (Polymerase Chain Reaction-PCR) methods. For this purpose, Xpert HPV Test (Cepheid, Inc, Sunnyvale, CA) was used to identify HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 t in a single sample. Results: Mean age of the patients ranged from 20 to 69 years, with a mean of 39.8 years (± 10.0). Positivity was detected in 62 of the 433 patients. Mean age of the positive patients was 40.2 years (± 11.3). When the positive patients were examined in terms of HPV types, the presence of HPV 16 was observed with a rate of 25.6%, while the HPV 18/45 types were found to be 9.0% in total. When patients were evaluated according to age groups, HPV DNA positivity was highest in the 25-34 age group with 38.7%. In our statistical study, there was no significant difference in HPV DNA positivity rate between the ages of 35 and under 35 years. Conclusion: This study demonstrates the prevalence and viral genotype distribution of HPV infection in women in Erzurum region. HPV type 16 is seen with a high rate in our region.

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Prevalence and genotype distribution of HPV infection among 214,715 women from Southern China, 2012-2018: baseline measures prior to mass HPV vaccination

10.21203/rs.3.rs-20658/v2 ◽

2020 ◽

Author(s):

Lipei Luo ◽

Ping He ◽

qiaotu liu ◽

Yanghua jiang ◽

Yang-nan Zhang ◽

...

Keyword(s):

Young Women ◽

Southern China ◽

Cervical Screening ◽

Hpv Vaccination ◽

Hpv Infection ◽

Cross Sectional Study ◽

Genotype Distribution ◽

Cross Sectional ◽

Hpv Prevalence ◽

Hpv Genotypes

Abstract Background: The epidemiology on the human papillomavirus (HPV) among females in Southern China is not well-established. Baseline data on the prevalence of HPV infection in China prior to mass prophylactic HPV vaccination would be useful. Thus, this study aimed to determine the type-specific HPV prevalence and distribution among females from Southern China prior to mass HPV vaccination. Methods: A retrospective cross-sectional study employing 214,715 women attending ChenZhou NO.1 People’s Hospital for cervical screening during 2012-2018 was conducted prior to widespread HPV vaccination. HPV genotype was detected using nucleic acid molecular diversion hybridization tests. The overall prevalence, age-specific prevalence, type distribution, and annual trend were analyzed. Results: The overall HPV prevalence was 18.71% (95% confidence interval [CI], 18.55%-18.88%) among Southern China females. During 2012–2018, the prevalence of HPV infection showed a downward tendency, from 21.63% (95% CI, 21.07%-22.20%) in 2012 to 18.75% (95% CI, 18.35%-19.16%) in 2018. Age-specific HPV distribution displayed a peak at young women aged less than 21 years (33.11%, 95% CI, 31.13%-35.15%), 20.07% (95% CI, 19.70%-20.44%) among women aged 21-30 years, 17.29% (95% CI, 17.01%-17.57%) among women aged 31-40 years, 17.23% (95% CI, 16.95%-17.51%) among women aged 41-50 years, 21.65% (95% CI, 21.11%-22.20%) among women aged 51-60 years, and 25.95% (95% CI, 24.86%-27.07%) among women aged over 60 years. Of the 21 subtypes identified, the most three prevalent high-risk HPV (HR-HPV) genotypes were HPV52 (5.12%; 95% CI, 21.11%-22.20%), -16 (2.96%; 95% CI, 2.89%-3.03%), and -58 (2.51%; 95% CI, 2.44%-2.58%); the predominant low-risk HPV (LR-HPV) genotypes were HPV81 (1.86%; 95%CI, 1.80%-1.92%) and -6 (0.69%; 95% CI, 0.66%-0.73%) respectively. Incidence of HR-HPV only, LR-HPV only and mixed LR- and HR-HPV were 15.17%, 2.07% and 1.47% respectively. Besides, single HPV infection accounted for 77.30% of all positive cases in this study.Conclusions: This study highlights 1) a high prevalence of HPV infection among females with a decreasing tendency towards 2012-2018, especially for young women under the age of 21 prior to mass HPV vaccination; 2) HPV52, -16 and -58 were the predominant HPV genotypes, suggesting potential use of HPV vaccine covering these HPV genotypes in Southern China.

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