The Impact of ymoA Gene Expression on Enterotoxin YstA Production by Yersinia Enterocolitica Strains With Different Enterotoxic Properties

2021 ◽  
Author(s):  
Agata Bancerz-Kisiel ◽  
Karolina Lipczyńska-Ilczuk
Keyword(s):  
Yersinia Enterocolitica ◽  
Clinical Signs ◽  
Expression Level ◽  
Relative Expression ◽  
Source Of Infection ◽  
Relative Expression Level ◽  
Genes Expression ◽  
Causative Agents ◽  
The Impact ◽  
Mrna Expression Levels

Abstract Yersinia enterocolitica is one of the main causative agents of human diarrhoea and the reservoir and source of infection for humans are pigs. Strains isolated from humans with clinical yersiniosis and diarrhoea are able to produce Yersinia stable toxins – Yst. However, enterotoxin-producing capabilities have been attributed to the ymoA gene which encodes the production of the Yersinia modulator protein – YmoA. The aim of this study was to analyse ystA and ymoA genes expression in Y. enterocolitica strains with different enterotoxic properties, isolated from humans and pigs. The experiment involved two groups of Y. enterocolitica strains producing and not producing enterotoxin YstA, which were isolated from humans and pigs. All these strains were ystA- and ymoA-positive. The relative expression level of the ystA gene was significantly higher than the expression level of the ymoA gene in Y. enterocolitica strains isolated from humans with clinical signs characteristic for yersiniosis. In others, a significant decrease in ystA gene transcription was observed, and the relative expression level of the ymoA gene was significantly higher than the expression level of the ystA gene. Statistically significant differences were not observed in either group of strains isolated from pigs. The results of our study revealed a correlation between the mRNA expression levels of ystA and ymoA genes in Y. enterocolitica strains isolated from humans.

scholarly journals Evaluation of the Correlation between the mRNA Expression Levels of ystA and ymoA Genes in Y. enterocolitica Strains with Different Enterotoxic Properties

Pathogens ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1136
Author(s):  
Agata Bancerz-Kisiel ◽  
Karolina Lipczyńska-Ilczuk
Keyword(s):  
Mrna Expression ◽  
Clinical Symptoms ◽  
Expression Level ◽  
Common Source ◽  
Expression Levels ◽  
Relative Expression ◽  
Source Of Infection ◽  
Relative Expression Level ◽  
Causative Agents ◽  
Mrna Expression Levels

Yersinia enterocolitica is one of the main causative agents of human diarrhea. Pigs are a reservoir and the most common source of infection for humans. The aim of this study was to analyze the expression of ystA and ymoA genes in Y. enterocolitica strains with different enterotoxic properties, isolated from humans and pigs. The experiment involved two groups of Y. enterocolitica strains producing and not producing enterotoxin YstA, which were isolated from humans and pigs. All strains were ystA- and ymoA-positive. The expression of ystA and ymoA genes was analyzed by quantitative real-time PCR (qPCR). The relative expression level of the ystA gene was significantly higher than the expression level of the ymoA gene in Y. enterocolitica strains isolated from humans with clinical symptoms of yersiniosis. In other strains, a significant decrease in ystA gene transcription was observed, and the relative expression level of the ymoA gene was significantly higher than the expression level of the ystA gene. Statistically significant differences were not observed in either group of strains isolated from pigs. The results of our study revealed a correlation between mRNA expression levels of ystA and ymoA genes in Y. enterocolitica strains isolated from humans.

scholarly journals IL-1βin Irrigation Fluid and MRNA Expression in Synovial Tissue of the Knee Joint as Therapeutic Markers of Inflammation in Collagen Antibody-Induced Arthritis

2009 ◽  
Vol 26 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Wei-Tso Chia ◽  
Li-Tzu Yeh ◽  
Yuan-Wu Chen ◽  
Herng-Sheng Lee ◽  
Deh-Ming Chang ◽  
...  
Keyword(s):  
Knee Joint ◽  
Synovial Tissue ◽  
Acute Stage ◽  
Expression Level ◽  
Enzyme Linked Immunosorbent Assay ◽  
Histopathological Changes ◽  
Irrigation Fluid ◽  
Relative Expression ◽  
Relative Expression Level ◽  
Markers Of Inflammation

Objective: We studied the relationship between the severity of inflammation and IL-1βproduction and relative expression level of IL-1βmRNA in irrigation fluid and synovial tissue obtained from the knee joint during the acute stage of a murine model of type II collagen antibody-induced arthritis (CAIA). This model is used to identify potential therapeutic markers for treating rheumatoid arthritis.Methods: Irrigation fluid and synovium tissue were harvested from the knee joint of BALB/c mice in acute stage of CAIA induction. The IL-1βprotein level was measured by enzyme-linked immunosorbent assay, and the relative expression level of IL-1βmRNA was analyzed by reverse transcription-polymerase chain reaction. Two investigators analyzed expression levels and histopathological changes.Results: IL-1βconcentration was higher in irrigation fluid from the knee joint than in the serum in the acute stage of CAIA. The relative expression level of IL-1βmRNA was elevated in synovial tissue. Histopathological changes in the knee joint and foot indicated similar severity.Conclusions: IL-1βconcentration in irrigation fluid and relative expression level of IL-1βmRNA in the synovium have potential as therapeutic markers in studying and treating CAIA.

scholarly journals OsBRKq1, Related Grain Size Mapping, and Identification of Grain Shape Based on QTL Mapping in Rice

2021 ◽  
Vol 22 (5) ◽  
pp. 2289
Author(s):  
Jae-Ryoung Park ◽  
Dany Resolus ◽  
Kyung-Min Kim
Keyword(s):  
Grain Size ◽  
Qtl Mapping ◽  
Grain Weight ◽  
Expression Level ◽  
Chromosome 1 ◽  
Gene Location ◽  
World Population ◽  
Genetic Traits ◽  
Relative Expression ◽  
Relative Expression Level

The world population is growing rapidly, and food shortage remains a critical issue. Quantitative trait locus (QTL) mapping is a statistical analytical method that uses both phenotypic and genotypic data. The purpose of QTL mapping is to determine the exact gene location for various complex traits. Increasing grain weight is a way to increase yield in rice. Genes related to grain size were mapped using the Samgang/Nagdong double haploid (SNDH) populations. Grain sizes were diversely distributed in SNDH 113 populations, and OsBRKq1 was detected on chromosome 1 in an analysis of QTL mapping that used 1000 grain weight, grain length, and grain width. OsBRKq1 exhibited high sequence similarity with the brassinosteroid leucine-rich repeat-receptor kinases of Arabidopsis thaliana and Zea mays. It was also predicted to have a similar function because of its high homology. OsBRKq1 interacts with various grain-size control genes. Among the SNDH populations, the analysis of the relative expression level during the panicle formation stage of OsBRKq1 in panicles of SNDH117, which has the largest grain size, and SNDH6, which has the smallest grain size, the relative expression level was significantly increased in SNDH117 panicles. SNDH populations have been advancing generations for 10 years; various genetic traits have been fixed and are currently being used as bridging parents. Therefore, the stable expression level of OsBRKq1 was confirmed via QTL mapping. In the future, OsBRKq1 can be effectively used to increase the yield of rice and solve food problems by increasing the size of seeds.

scholarly journals Evaluation of level of expression of microRNA-21 and let-7g in serum and stool of patients with colorectal cancer

2021 ◽  
Author(s):  
Dara Rahmanpour ◽  
Roya Dolatkhah ◽  
Soheil Navidizadeh ◽  
Peyman Virani ◽  
Nastran Hasanzadeh ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Expression Patterns ◽  
Rna Extraction ◽  
Expression Level ◽  
Serum Samples ◽  
Significance Level ◽  
Relative Expression ◽  
Relative Expression Level ◽  
Stool Samples

Abstract Background MicroRNAs (miRs) are involved in the pathogenesis of various malignancies such as colorectal cancer through regulating multiple cellular processes, including cell proliferation, cell cycle, apoptosis and migrationMiR-21 and let-7 are two important genes that have confirmed in this pathway. The role of the let-7 gene as a gene tumor process in various cancers and the role of miR-21 in the development and progression of cancer has been conclusively identified also this gene has an oncogenic role in various cancers. In this study, the expression patterns of miR-21 and let-7 in serum and stool samples of colorectal cancer patients were evaluated. Materials and Methods During the present study, 120 samples including 40 serum samples of CRC and 40 stool samples from the same patients and 40 healthy samples were collected. After total RNA extraction, real-time PCR was used to measure changes in genes expression. Statistical analysis of data was performed with GraphPad Prism statistical software (Version 6.0) with a significance level of 5%. Results The relative expression level of miR-21 in the serum samples of CRC increased compared to the healthy group, which was statistically significant. On the other hand, the relative expression level of let-7g in the serum samples of CRC showed a significant decrease compared to the healthy sample. In stool samples, the expression changes of either of the two genes were not significant. Conclusion Our findings indicate that the relative expression of miR-21 and let-7g genes can be used as a diagnostic or predictive biomarker in colorectal cancer serum samples. While, this is not the case in stool samples. Moreover, further investigations at the protein level should be performed.

Dopamine receptors genes activity under chronic stress in rats

Biomics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 1-7
Author(s):  
E.V. Valeeva ◽  
I.Kh. Valeeva ◽  
I. I. Semina ◽  
D.O. Nikitin ◽  
A.G. Mukhamedzhanova ◽  
...  
Keyword(s):  
Stress Response ◽  
Dopamine Receptors ◽  
Chronic Stress ◽  
Peripheral Blood ◽  
Immobilization Stress ◽  
Brain Structures ◽  
Expression Level ◽  
Relative Expression Level ◽  
Genes Expression ◽  
Negative Effect

Stress response is a multifactorial condition which is formed under extreme environmental exposure due to various neuroendocrine systems interactions. Dopaminergic system plays a key role in stress response through the dopamine which effect is realized after binding with special dopamine receptors types D1-D5. Expression of these receptors varies in different tissues, organs and specific brain structures but there is a special interest in their genes expression level in peripheral blood that can be served as additional marker to evaluate the chronic stress degree. Herein we determine the influence of various types induced chronic stress exposure during 6 months (such as exhausting physical activity (forced swimming), immobilization stress, and their combinations (swimming with immobilization)) on Drd1, Drd2 and Drd3 genes expression level in Wistar rats’ peripheral blood. According to our results, no activity for Drd2 and Drd3 genes is shown; however significant overexpression of Drd1 gene was detected in all studied groups after 3 months exposure compared to the data before experiment beginning, whereas after 6 months the relative expression level significantly decreased in the group with immobilization stress, which proves the negative effect of this type of chronic stress on the production of dopamine receptors of the D1 type. Thus, Drd1 gene activity in the blood can serve as a marker for assessing the severity of chronic stress in rats.

Synergic Effects of Immunosuppressive Agents on T Lymphocyte Function and Cytokine Expression Pattern.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 5213-5213
Author(s):  
Mao Fang Lin ◽  
Yan Ling Jiang
Keyword(s):  
Protein Expression ◽  
Mrna Expression ◽  
T Lymphocyte ◽  
Immunosuppressive Agents ◽  
Reaction System ◽  
Expression Level ◽  
Lymphocyte Function ◽  
Relative Expression ◽  
Relative Expression Level ◽  
Ifn Γ

Abstract The regimen including mycophenolate mofetil (MMF) and cyclosporine A (CsA) has been successfully used as an effective method in the prophylaxis of acute graft versus host disease. Herewith we investigated the effect of CsA, mycophenolic acid (MPA) alone or in combination with anti B7–1 mAb on proliferation of T lymphocytes and their possible mechanisms. The T lymphocyte reaction system was established in vitro and was detected by 5-bromo-2-deoxyuridine incorporation method; the expression of IL-2, IFN-γ and IL-10 on mRNA and protein level were detected by RT-PCR and ELISA respectively. Experimental concentration of MPA (50μmol/L) and CsA(0.33μmol/L) inhibited the T lymphocyte proliferation significantly. CsA in combination with anti B7–1 mAb(10mg/L) had a much stronger effect on the inhibition(p<0.01). There was no difference between groups treated either with MPA or with the combination of MPA and anti B7–1 mAb. Both of CsA and MPA could inhibit the protein expression of IL-2 and IFN-γ. The protein expression of IL-2 were 99.70±9.15 pg/ml, 48.19±8.67pg/ml and 42.73±14.64 pg/ml in groups of control, treated with CsA or MPA respectively. The protein expression of IFN-γ in the group treated with CsA or MPA decreased significantly than the control (6.81±5.24 pg/ml, 7.87±4.22 pg/ml vs 82.42±25.55 pg/ml, p<0.05). Compared with that treated with CsA alone, The protein expression of IFN-γ in the group treated with CsA in combination with anti B7–1 mAb decreased significantly (0.30±0.52 pg/ml vs 6.81±5.24 pg/ml, p<0.05). Both of CsA and MPA increased the protein expression of IL-10. Compared with MPA alone, MPA in combination with anti B7–1 mAb increased significantly the expression of IL-10 (941.90±56.61 pg/ml vs 770.95±126.85 pg/ml, p<0.05). The relative expression level of IL-2 mRNA (IL-2/GAPDH) and IFN-γ mRNA (IFN-γ /GAPDH) in the CsA or MPA group was significantly lower than the control (p<0.01). The relative expression level of IFN-γ mRNA in combination group of CsA with anti B7–1 mAb decreased even more significantly (0.38±0.05 vs 0.59±0.02, p<0.01). On the other hand, this combination could up-regulate IL-10 mRNA expression (1.80±0.13 vs 1.38±0.06, p<0.01). Combination of MPA with anti-B7–1 mAb showed a statistically significant increase in IL-10 mRNA expression (1.28±0.06 vs 0.84±0.09, p<0.01) as compared with MPA alone. In conclusion, MPA and CsA induced the changes of cytokine expressive spectrum and the Th1 and Th2 shift might be involved in the immunosuppressive effect. The combination of immunosuppressive agents, especially with anti B7–1 mAb had a synergic effect.

scholarly journals EFFECT OF HUMAN ADIPOSE-DERIVED STEM CELL IN COLLAGEN GEL ON RELATIVE EXPRESSION LEVEL OF VASCULAR ENDOTHELIAL GROWTH FACTOR-A OF DEEP DERMAL BURN HEALING

2018 ◽  
Vol 11 (7) ◽  
pp. 383
Author(s):  
Puji Sari ◽  
Dwi Pratami Septiara ◽  
Helsy Junaidi ◽  
Luluk Yunaini ◽  
Jenanne A Pawitan
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Stem Cell ◽  
Collagen Gel ◽  
Expression Level ◽  
Vascular Endothelial ◽  
Relative Expression ◽  
Relative Expression Level ◽  
And Control ◽  
Endothelial Growth Factor ◽  
Adipose Derived Stem Cell

Objectives: The objectives of this research was to measure the relative expression levels of vascular endothelial growth Factor-A (VEGF-A) in the deep dermal burns treated with human adipose-derived stem cell (hADSC) in collagen gel in each observational day (days 7, 14, 21, and 28).Methods: This study used 20 male Sprague Dawley rats, divided into four groups of observation days. Each rat received three burn injuries and then given different treatments (hADSC in collagen gel, collagen gel, and control). Deep dermal burn injury on the dorsal was made by placing a metal plate with 250°C for 15 s. Relative expression level of VEGF-A measurement with quantitative reverse transcription polymerase chain reaction.Results: On the 7th day, the relative expression level of VEGF-A in the wound treated with hADSC in collagen gel was significantly different from the scaffold collagen and control group (p<0.05), whereas the control and scaffold collagen group was not significantly different. The relative expression level of VEFG-A in wound treated with hADSC in collagen gel, collagen gel only, and control was (mean±standard error of the mean) 17.93±4.37, 7.54±2.63, and 5.44±1.59, respectively. On the next observation days, the result showed that the relative expression level of VEGF-A was not significantly different between the three treatments. The relative expression level of VEGF-A has decreases from day 7 to 28 days. The decrease of VEGF-A relative expression level hADSC in collagen gel group was significantly different on the 7th day to the 21st and 28th days (p<0.05).Conclusion: The provision of hADSC in scaffold collagen increases the relative expression of VEGF-A early in the wound healing process compared to the without a hADSC group.

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