scholarly journals Genome-wide analysis of CrRLK1L genes and their expression profiling during fiber development in cotton

2020 ◽  
Author(s):  
Dongyun Zuo ◽  
Javaria Ashraf ◽  
Hailiang Cheng ◽  
Shang Liu ◽  
Youping Zhang ◽  
...  

Abstract Background: Catharanthus roseus receptor-like kinase 1-like (CrRLK1Ls) proteins play important roles in cell growth, plant morphogenesis, reproduction, hormone signaling, plant immunity and stress responses in Arabidopsis. However, not much information is available about their functions during cotton fiber development.Results: We identified a total of 125, 73 and 71 full-length putative CrRLK1L genes in G. hirsutum, G. arboreum and G. raimondii, which are much greater than that of the other plants. The phylogenetic and gene structure analysis divided the cotton CrRLK1L genes into six major groups, among which only group I and II contained AtCrRLK1Ls of Arabidopsis, suggesting that other groups (group III-VI) were expanded by gene duplication during cotton evolution. Genome collinearity analysis revealed that half of the At02 genes in G. hirsutum derived from A02 of G. arboreum, while the other half (GhCrRLK1L6 and GhCrRLK1L7) originated from Dt03 and Dt02 of G. raimondii, indicating segmental duplication between noncorresponding chromosomes during polyploidization of G. hirsutum. In addition, expression and cis-element analysis revealed that only 22 GhCrRLK1Ls showed specific expression pattern during fiber development which are mainly due to the presence of binding sites for NAC, MYB and WRKY transcription factors.Conclusions: This study provides a strong foundation to further explore the molecular mechanism of CrRLK1L genes during fiber development in upland cotton.

1960 ◽  
Vol 11 (1) ◽  
pp. 75 ◽  
Author(s):  
M Wodzicka

The monthly wool growth of three groups of rams was studied at Beltsville, Maryland. Group I received natural daylight (at 38° 53' N.) and was shorn monthly. Group II had a 7:17 hours of daylight to hours of darkness rhythm and was shorn every 6 months, once in winter and once in summer. Group III received natural daylight and was likewise shorn every 6 months. The rams of all groups produced more wool in summer than in winter. This difference was significant (P<0.001). The mean body weight and food intake were both greater in the winter months, which indicated that the seasonal rhythm of wool growth was not a consequence of poorer feeding in winter. The rams which were shorn monthly (group I) grew considerably more wool than the other two groups, but the difference was not statistically significant. The short-day treatment of group II did not increase the annual wool production nor decrease the seasonal rhythm of wool growth. The balance of evidence from this and other experiments indicates that temperature rather than light controls the seasonal rhythm of wool growth.


1985 ◽  
Vol 248 (5) ◽  
pp. F711-F719 ◽  
Author(s):  
J. C. Ayus ◽  
R. K. Krothapalli ◽  
D. L. Armstrong

The purpose of the present studies was to examine the effects of rapid correction of severe hyponatremia (serum sodium less than 120 meq/liter) either to mildly hyponatremic levels (serum sodium = 130 meq/liter) or to normonatremic levels (serum sodium = 150 meq/liter) on the brain histology of rats. In group I, 13% of the rats revealed brain lesions following correction to mildly hyponatremic levels by the administration of 855 mM NaCl. All the rats (100%) in group II had brain lesions following correction to normonatremic levels by 24 h of water restriction. Similarly, all the rats in group III showed brain lesions following correction to normonatremic levels by the administration of 855 mM NaCl. Severe hyponatremia by itself did not cause any brain lesions in another group. We conclude that rapid correction of severe hyponatremia to mildly hyponatremic levels by the administration of 855 mM NaCl does not cause significant brain lesions. On the other hand, rapid correction to normonatremic levels either by water restriction or by the administration of 855 mM NaCl results in significant brain lesions.


2019 ◽  
Vol 28 (4) ◽  
pp. 101
Author(s):  
Aneta Michalska-Warias

<p>The article is devoted to the analysis of court punishment practice in the case of offences which have the same statutory punishment. Three groups of such offences are selected: offences punished with imprisonment from 2 to 12 years (group I), offences punished with imprisonment from 3 months to 5 years (group II) and offences punished with imprisonment from 1 month to 3 years (group III). Most of the analysed offences belonged to the group of offences against freedom (including sexual freedom) and the other chosen offences were against other socially cherished values were those quite popular in practice (therefore, the statistical data in their cases are quite representative). The analysed year was 2016. The starting hypothesis was that offences which have identical punishments in the Criminal Code (which means that the lawmaker perceives them as socially harmful in a similar way) will not be treated in such a similar way in practice and in all groups there would be offences which would be punished with visibly more severe and lighter punishments. Detailed analysis of statistical data referring to punishments imposed for the discussed offences confirmed the initial hypothesis, showing also the already known fact that courts tend to impose punishments which are closer to the minimum than to the maximum provided by the lawmaker.</p>


F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 1038 ◽  
Author(s):  
Samaa Kotb ◽  
Atef Shaker ◽  
Carl Halim

Background: Treatment of patients with severe tooth wear is complex. Treatments involving more tooth structure removal may be inappropriate for patients who have already lost a significant amount of dental tissue due to erosion. The aim of this study was to evaluate the effect of two modified occlusal veneer preparations on the fatigue resistance and stress distribution of bonded occlusal veneers, in comparison to the conventional preparation design. Methods: A total of 54 human mandibular molars were distributed into three equal groups of n=18 teeth each, according to the occlusal veneer preparation design, where group I (conventional design) received Planar occlusal veneer preparation, group II (occlusal veneer with circumferential finish line) and group III (occlusal veneer with intracoronal cavity extension). For the manufacture of all the veneers using machinable zirconium lithium silicate glass ceramic blocks, a computer aided design/manufacturing system was used. A dual cure, adhesive resin cement was used to bond all occlusal veneers to corresponding prepared teeth. After storage in water for one week, step-stress (accelerated life testing was performed for all samples. Finite element analysis was carried out as well to evaluate the distribution of stresses. Results: The highest values (mean±SD) were recorded for group II (890.57±211.53 N) followed by Group I values (883.54±135.91 N), while the lowest values were recorded for Group III (875.57±143.52 N). The difference between groups was statistically non-significant as indicated by ANOVA (P=0.9814>0.05). The stress values were generally found to be low and their distribution differed among groups. Conclusion: Group I and II showed comparable fatigue resistance and more favourable failure behaviour when compared to Group III based on the fractographic and 3D finite element analyses.


2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Yuzhu Huo ◽  
Wangdan Xiong ◽  
Kunlong Su ◽  
Yu Li ◽  
Yawen Yang ◽  
...  

The plant-specific transcription factor TCPs play multiple roles in plant growth, development, and stress responses. However, a genome-wide analysis of TCP proteins and their roles in salt stress has not been declared in switchgrass (Panicum virgatum L.). In this study, 42 PvTCP genes (PvTCPs) were identified from the switchgrass genome and 38 members can be anchored to its chromosomes unevenly. Nine PvTCPs were predicted to be microRNA319 (miR319) targets. Furthermore, PvTCPs can be divided into three clades according to the phylogeny and conserved domains. Members in the same clade have the similar gene structure and motif localization. Although all PvTCPs were expressed in tested tissues, their expression profiles were different under normal condition. The specific expression may indicate their different roles in plant growth and development. In addition, approximately 20 cis-acting elements were detected in the promoters of PvTCPs, and 40% were related to stress response. Moreover, the expression profiles of PvTCPs under salt stress were also analyzed and 29 PvTCPs were regulated after NaCl treatment. Taken together, the PvTCP gene family was analyzed at a genome-wide level and their possible functions in salt stress, which lay the basis for further functional analysis of PvTCPs in switchgrass.


1981 ◽  
Author(s):  
P Glas-Greenwalt ◽  
M H Goldman

To determine the importance of the fibrinolytic system in renal transplantation on the one hand, and to establish a correlation between possible endothelial damage due to treatment of the renal graft and fibrinolytic parameters on the other, dogs were divided in six groups. Group I dogs were subjected to anesthesia only. Group II was sham operated. In group III, kidneys were perfused with the supernatant of either autologous or homologous cryo-precipitated plasma, and in group IV with albumin. In group V kidneys were cold stored. This was followed by autotransplantation. In group VI kidneys were perfused with albumin and allografted. Before and after transplantation, total plasma plasminogen (pro) activator activities in systemic and renal circulations were measured on fibrin plates after the addition of dextran sulfate and flufenamate to euglobulin fractions. Vascular activator (VA) was measured by adding Cl-inactivator. There was no marked difference between groups III, IV and VI. In comparing, however, group V with any of the perfused groups, an overall higher fibrinolytic activity was recorded both for intrinsic activators (p<.001) and VA (p<.001). in group I a significant drop in both activities (p<.01 -<.02) could be directly related to the duration of anesthesia with recovery within 24 hours. This pattern, though highly accentuated, revealed itself in all the other groups studied, with VA temporarily reaching zero-levels in the renal circulation in group VI. This data indicates the participation of the fibrinolytic system, in particular of the VA, in determining the fate of renal grafts.


1969 ◽  
Vol 11 (4) ◽  
pp. 569-572
Author(s):  
M. A. Sharafeldin ◽  
I. A. Ramadan

SUMMARYAt birth, the Barki lambs used in this experiment were randomly allocated to three groups which numbered 133, 126 and 154 lambs of both sexes weaned at the ages of 10 weeks (Group I), 12 weeks (Group II) and 16 weeks (Group III) respectively.The most pronounced differences between the three groups of lambs in their body weights took place at the age from 4 to 6 months. Group II lambs performed as well as or even slightly better than the other two groups, which leads to the conclusion that as far as the body weight of lambs is concerned there is no need to extend their suckling period more than 12 weeks.The three groups of lambs varied little in their mortality rates from 2·5 to 12 months of age.


2013 ◽  
Vol 2013 ◽  
pp. 1-7
Author(s):  
Hülya Güngel ◽  
Asiye Nurten ◽  
İhsan Kara ◽  
Serife Evrim Kepekci Tekkeli ◽  
Elif Özkök ◽  
...  

The effects of the ischemia and reperfusion on the lens metabolites and the effect of a phytotherapeutic commercial product called “Enoant” (mixed polyphenol content) on the selected lens metabolites were investigated. For this aim, 30 Wistar rats were divided into three groups according to their diet and being subjected to ischemia. 10 of the rats as Group I were fed on dry diet; the other 10 (Group II) were fed on dry diet and drinking water with Enoant. At the end of 15 days period, both groups of rats were subjected to ischemia for 2 hours and reperfused. After another 15 days with their same diet, the rats were decapitated. The remaining 10 rats, which were not subjected to ischemia (Group III), were fed on dry diet only. 1HNMR spectroscopy was used for the determination of lens metabolites of each group of rats. The results obtained from the three groups have been compared statistically. The differences of metabolites were significant except pyruvate and succinate. Oral administration of Enoant revealed effects on increasing membrane stabilization, the antioxidant capacity, osmotic regulator molecule capacity, and sorbitol content of lens disturbed by ischemia. Enoant can be used where oxidative or osmotic stress is formed.


BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Jiseong Jeong ◽  
Sunhee Park ◽  
Jeong Hui Im ◽  
Hankuil Yi

Abstract Background The Gretchen Hagen 3 (GH3) genes encode acyl acid amido synthetases, many of which have been shown to modulate the amount of active plant hormones or their precursors. GH3 genes, especially Group III subgroup 6 GH3 genes, and their expression patterns in economically important B. oleracea var. oleracea have not been systematically identified. Results As a first step to understand regulation and molecular functions of Group III subgroup 6 GH3 genes, 34 GH3 genes including four subgroup 6 genes were identified in B. oleracea var. oleracea. Synteny found around subgroup 6 GH3 genes in B. oleracea var. oleracea and Arabidopsis thaliana indicated that these genes are evolutionarily related. Although expression of four subgroup 6 GH3 genes in B. oleracea var. oleracea is not induced by auxin, gibberellic acid, or jasmonic acid, the genes show different organ-dependent expression patterns. Among subgroup 6 GH3 genes in B. oleracea var. oleracea, only BoGH3.13–1 is expressed in anthers when microspores, polarized microspores, and bicellular pollens are present, similar to two out of four syntenic A. thaliana subgroup 6 GH3 genes. Detailed analyses of promoter activities further showed that BoGH3.13–1 is expressed in tapetal cells and pollens in anther, and also expressed in leaf primordia and floral abscission zones. Conclusions Sixty-two base pairs (bp) region (− 340 ~ − 279 bp upstream from start codon) and about 450 bp region (− 1489 to − 1017 bp) in BoGH3.13–1 promoter are important for expressions in anther and expressions in leaf primordia and floral abscission zones, respectively. The identified anther-specific promoter region can be used to develop male sterile transgenic Brassica plants.


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