Diazinon Reduction In Apple Juice Using Probiotic Bacteria During Fermentation And Refrigerated Storage

2021 ◽  
Author(s):  
Farahnaz Rezaei ◽  
Roghayeh Nejati ◽  
Mehran Sayadi ◽  
Amene Nematillahi
Keyword(s):  
Cold Storage ◽  
Apple Juice ◽  
Solid Phase ◽  
Sugar Content ◽  
Chemical Properties ◽  
Storage Period ◽  
Probiotic Bacteria ◽  
Gas Chromatography Mass Spectrometry ◽  
Reducing Ability ◽  
Probiotic Strains

Abstract The main objective of this work was to study the effects of probiotic strains, probiotic primary inoculated population, concentrations of spiked diazinon, physiology of probiotic bacteria, fermentation times and cold storage period in six consecutive stages on diazinon reduction in apple juice. Chemical properties (pH, total acidity, and sugar content), probiotic viability and diazinon reduction percent were monitored during fermentation and cold storage. Dispersive Solid Phase Extraction (dSPE) followed by gas chromatography-mass spectrometry was used to extract and measure diazinon concentration. Results showed that Lactobacillus acidophilus revealed the highest ability to reduce diazinon in apple juice after fermentation. Inoculation of L. acidophilus at 9 log CFU/mL, showed significantly higher diazinon reducing ability than 7 log CFU/mL. Ability of L. acidophilus to reduce the concentration of 1000 µg/L of spiked diazinon was significantly greater than 5000 µg/L. Heat-killed (dead) L. acidophilus bacteria reduced less diazinon content at the end of fermentation than viable bacteria. Furthermore, 72 h of fermentation was more effective in diazinon reduction. Level of diazinon in treatments containing L. acidophilus decreased significantly during cold storage period, so that it is completely disappeared at the end of storage (28 days) along with maintaining health-promoting properties of probiotic apple juice.

scholarly journals Transcriptome Analysis of Pre-Storage 1-MCP and High CO2-Treated ‘Madoka’ Peach Fruit Explains the Reduction in Chilling Injury and Improvement of Storage Period by Delaying Ripening

2021 ◽  
Vol 22 (9) ◽  
pp. 4437
Author(s):  
Han Ryul Choi ◽  
Min Jae Jeong ◽  
Min Woo Baek ◽  
Jong Hang Choi ◽  
Hee Cheol Lee ◽  
...  
Keyword(s):  
Cold Storage ◽  
Transcriptome Analysis ◽  
Molecular Mechanisms ◽  
Chilling Injury ◽  
Chemical Properties ◽  
Storage Period ◽  
Differentially Expressed ◽  
Peach Fruit ◽  
High Co2 ◽  
Physico Chemical

Cold storage of peach fruit at low temperatures may induce chilling injury (CI). Pre-storage 1-MCP and high CO2 treatments were reported among the methods to ameliorate CI and reduce softening of peach fruit. However, molecular data indicating the changes associated with pre-storage 1-MCP and high CO2 treatments during cold storage of peach fruit are insufficient. In this study, a comparative analysis of the difference in gene expression and physico-chemical properties of fruit at commercial harvest vs. stored fruit for 12 days at 0 °C (cold-stored (CS), pre-storage 1-MCP+CS, and pre-storage high CO2+CS) were used to evaluate the variation among treatments. Several genes were differentially expressed in 1-MCP+CS- and CO2+CS-treated fruits as compared to CS. Moreover, the physico-chemical and sensory data indicated that 1-MCP+CS and CO2+CS suppressed CI and delayed ripening than the CS, which could lead to a longer storage period. We also identified the list of genes that were expressed commonly and exclusively in the fruit treated by 1-MCP+CS and CO2+CS and compared them to the fruit quality parameters. An attempt was also made to identify and categorize genes related to softening, physiological changes, and other ripening-related changes. Furthermore, the transcript levels of 12 selected representative genes from the differentially expressed genes (DEGs) in the transcriptome analysis were confirmed via quantitative real-time PCR (qRT-PCR). These results add information on the molecular mechanisms of the pre-storage treatments during cold storage of peach fruit. Understanding the genetic response of susceptible cultivars such as ‘Madoka’ to CI-reducing pre-storage treatments would help breeders release CI-resistant cultivars and could help postharvest technologists to develop more CI-reducing technologies.

scholarly journals Application of Arabic Gum and Essential Oils as the Postharvest Treatments of Le Conte Pear Fruits during Cold Storage

2019 ◽  
pp. 1-11
Author(s):  
Malaka A. Saleh ◽  
Nagwa S. Zaied ◽  
M. A. Maksoud ◽  
Omaima M. Hafez
Keyword(s):  
Weight Loss ◽  
Fruit Quality ◽  
Cold Storage ◽  
Chemical Properties ◽  
Storage Period ◽  
Fruit Weight ◽  
Soluble Solids ◽  
Mature Stage ◽  
Arabic Gum ◽  
Moringa Oil

The present study was conducted during 2015 and 2016 seasons on Le Conte pear fruits harvested at mature stage, to investigate the application of (10% w/v) Arabic Gum (AG), Jojoba oil (JO) and Moringa oil (MO) at the rate (100 %) alone on physical and chemical properties. Fruits stored at 0 ± 1°C and 85-90 % relative humidity (RH) for 105 days. A fruit sample of each treatment was taken out at the end of cold storage period and left at room temperature (23 ± 2°C) and 47% (RH) up to 7 days was examined for quality Assessments. Fruit physical properties (weight loss, decay percentages and fruit firmness "Lb/inch2") and fruit chemical properties (total soluble solids percentage and total acidity percentage were evaluated. The results obtained that, all treatments including control succeeded in preventing fruit decay percentage up to 30 days in the two seasons. It is worth mentioning that, Le Conte pear fruits were coated with MO with stands free from deterioration up to 75 days of cold storage period. Beside, fruits coated MO progress in reducing the percentages of weight loss and decay throw cold storage periods for 105 day. The rate of softening increased in fruits with increasing the storage time in both fruits coated and uncoated, but it was significantly declined in uncoated treatments. Moreover, JO coating was more effective in firmness retention compared to the other treatments. Followed by pear fruits was coating with MO and AG in this respect. Furthermore, all coated fruits enhancement fruit quality during storage periods. Finally, it can be concluded that coating Le Conte fruits by Moringa oil recorded successfully reduction in fruit weight loss, decay percentages, improving fruit quality and extended storage fruit life as well as stimulate marketing period (shelf life), also safe on environmental and human health.

scholarly journals Effects of Different Postharvest Storage Methods on the Quality Parameters of Chestnuts (Castanea sativa Mill.)

HortScience ◽  
2015 ◽  
Vol 50 (4) ◽  
pp. 577-581 ◽  
Author(s):  
Engin Ertan ◽  
Esra Erdal ◽  
Gülsüm Alkan ◽  
Burak E. Algül
Keyword(s):  
Cold Storage ◽  
Starch Content ◽  
Sugar Content ◽  
Storage Period ◽  
Castanea Sativa ◽  
Total Sugar ◽  
Total Carbohydrate ◽  
Total Sugar Content ◽  
Fruit Samples ◽  
Total Starch

This trial was initiated in the harvest season of 2010 to determine the effects of traditional and cold storage on the fruit quality properties of chestnuts during the harvest and postharvest periods. Physical and biochemical analyses were conducted on fruit samples collected about once every 2 weeks from the middle of September until the end of December. Specifically, the shell and kernel colors (hue, chroma), water activity (aw), and total sugar (%), total starch (%), total carbohydrate (%), and tannin (ppm) contents were determined. Under traditional and cold storage conditions, the total sugar content of the chestnuts increased whereas the total starch content decreased during the storage period. In addition, the maximum tannin content was measured in fruit that was cold stored for a period of 60 days.

scholarly journals SPME-GC-MS and Multivariate Analysis of Sensory Properties of Cheese in a Sack Matured with Probiotic Starter Cultures

2020 ◽  
Vol 58 (2) ◽  
pp. 128-137
Author(s):  
Deni Kostelac ◽  
Ivančica Delaš ◽  
Jadranka Frece ◽  
Marko Jelić ◽  
Iva Čanak ◽  
...  
Keyword(s):  
Solid Phase ◽  
Starter Culture ◽  
Principal Component ◽  
Aroma Compounds ◽  
Sensory Properties ◽  
Starter Cultures ◽  
Probiotic Bacteria ◽  
Gas Chromatography Mass Spectrometry ◽  
Traditional Cheese ◽  
Principal Component Analyses

Research background. Cheese in a sack is a traditional cheese produced in Croatia. Types of cheese with similar production technology are made in other countries but chemical and microbiological composition varies between regions. Traditionally, cheese in a sack is produced without the addition of starter cultures. Addition of beneficial probiotic cultures to numerous dairy products has documented advantages. Effects that the addition of probiotic bacteria to traditional cheese have on aroma compounds and sensory properties have not been fully investigated. The aim of this study is to determine the sensory properties and differences in the aromatic profiles between cheese samples ripened in a lambskin sack, produced traditionally without the addition of any starter culture, or with the addition of probiotic bacteria. Experimental approach. In this study, cheese in a sack was produced with the addition of probiotic cultures Lactobacillus plantarum B and L. lactis ssp. lactis S1. During ripening volatile aroma compounds were analysed with a solid-phase microextraction gas chromatography-mass spectrometry. Sensory properties were evaluated by trained tasters who are familiar with the traditional taste of the cheese from a sack. The results of aroma composition and taste scores were then compared using factorial and principal component analyses. Results and conclusions. Chromatography showed differences in the composition of aroma compounds and the sensory properties between the cheese produced with Lactobacillus starter cultures and the control cheese, traditionally produced without a starter culture. The addition of probiotic cultures L. plantarum B and L. lactis ssp. lactis S1 resulted in products with better sensory properties and chemical profile of volatile aromatic compounds. Novelty and scientific contribution. This study investigates the usage of naturally present probiotic cultures as starter cultures in cheese in a sack production. Their effects on aroma profiles and sensory characteristics have been compared for the first time using factorial and principal component analyses.

scholarly journals Characterization of the Volatiles and Quality of Hybrid Grouper and Their Relationship to Changes of Microbial Community During Storage at 4 °C

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 818 ◽  
Author(s):  
Wenbo Huang ◽  
Jing Xie
Keyword(s):  
Microbial Community ◽  
16S Rrna ◽  
Microbial Activity ◽  
Free Amino ◽  
Solid Phase ◽  
Storage Period ◽  
Gas Chromatography Mass Spectrometry ◽  
Rrna Gene ◽  
Hybrid Grouper ◽  
Spoilage Bacteria

To investigate the effects of spoilage bacteria on aquatic product quality and volatile organic compounds (VOCs) in hybrid grouper (Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂), the physical conditions were evaluated, the chemical changes including color, total volatile base nitrogen (TVB-N), VOCs, and free amino acids (FFAs) were determined, and biological profiles were made through microbial community (total viable counts (TVC), 16S rRNA gene amplification sequencing, and next-generation sequencing (NGS) technology on hybrid grouper, which were stored at 4 °C for 10 days. The results showed that the whiteness and TVB-N of grouper increased throughout the storage period. The contents of glycine, alanine, and total free amino acid decreased with the microbial activity towards the end of the study period. At the end of storage, the TVC reached 9.0 log10 (CFU/g). Seventy eight strains of bacteria were isolated from the hybrid grouper, most of which were shown to be Pseudomonas spp., after 16S rRNA sequencing. The results of the NGS test showed that the diversity of dominant bacteria decreased with time; Pseudomonas azotoformans was the dominant spoilage bacteria at the end of storage. The VOCs of fish and bacteria in the grouper’s spoilage process were presented in headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS). Twenty eight compounds were identified in hybrid grouper, among which alcohol and aldehyde were used to characterize freshness, both of which were not only related to the overall flavor of the grouper, but were also affected by microbial activity. However, due to the complexity of microbial communities in aquatic products, the correlation between community changes and VOCs needs further research. This study provides insights into the correlation between VOCs and specific spoilage organisms (SSOs) through the analysis of the microbial community and VOCs.

scholarly journals Quality of kohlrabi stems (Brassica oleracea var. gongylodes L.) kept in cold storage

2011 ◽  
Vol 23 (2) ◽  
pp. 107-110 ◽  
Author(s):  
Maria Gawęda ◽  
Zofia Nizioł-Łukaszewska
Keyword(s):  
Ascorbic Acid ◽  
Brassica Oleracea ◽  
Cold Storage ◽  
Dry Matter ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Storage Period ◽  
Dry Matter Content

Quality of kohlrabi stems (Brassica oleracea var. gongylodes L.) kept in cold storage Two green kohlrabi cultivars, ‘White Delikates’ and ‘Korist’ F1, were kept in cold storage at a temperature of 2°C and a relative humidity of 95%. Natural mass losses were measured at monthly intervals and dry matter content, soluble sugars, L-ascorbic acid and isothiocyanates were analysed. During five months of storage, very low losses of kohlrabi mass were detected. The decrease in dry matter during that time was between 15 and 18%. After a brief increase, soluble sugar content decreased during storage, and in March, 50% of the initial sugar content was calculated for ‘Delikates’ kohlrabi flesh and 65% for ‘Korist’. L-ascorbic acid was well preserved in the kohlrabi, since 90% remained after storage was completed. The isothiocyanate content changed little and the vegetable remained a good source of these compounds throughout the storage period.

scholarly journals Genotype Is Primarily Responsible for Variance in Table Beet Geosmin Concentration, but Complex Genotype × Environment Interactions Influence Variance in Total Dissolved Solids

2019 ◽  
Vol 144 (6) ◽  
pp. 429-438 ◽  
Author(s):  
Solveig J. Hanson ◽  
Irwin L. Goldman
Keyword(s):  
Genetic Manipulation ◽  
Solid Phase ◽  
Chemical Properties ◽  
Total Dissolved Solids ◽  
Gas Chromatography Mass Spectrometry ◽  
Fertilizer Treatment ◽  
Dissolved Solids ◽  
F1 Hybrid ◽  
Genotype Effect ◽  
Split Plot

Earthy aroma and sweet flavor, conferred by the volatile terpenoid geosmin (trans-1,10-dimethyl-trans-9-decalol) and sucrose, respectively, are two essential flavor components of table beet (Beta vulgaris ssp. vulgaris). To elucidate the influence of genotype, growing environment, and fertilizer treatment on geosmin concentration and sucrose [as total dissolved solids (TDS)] in table beet, a field-based genotype × environment study was conducted using a split-split plot design. Four site × year combinations served as whole plots; MgSO4·H2O and CaSO4 comprised split plot fertilizer treatments; open-pollinated cultivars Bull’s Blood and Touchstone Gold, F1 hybrid Merlin, and inbred line W357B constituted split-split plot genotype treatments. Geosmin concentration was measured via gas chromatography–mass spectrometry using headspace solid-phase microextraction, and TDS was measured via refractometry. Variation in geosmin concentration was attributable to a strong genotype effect and significant genotype × year and year × site interactions. Genotypes were observed to have characteristic geosmin concentration and variance, despite being grown in soils with widely divergent physical and chemical properties. While a significant genotype main effect was also present for TDS, it occurred in the context of significant four-way and three-way genotype × environment interactions, plus significant effects of year and year × site interaction. Neither geosmin concentration nor TDS was significantly influenced by fertilizer treatment or fertilizer × environment interactions, averaged across genotypes. Genetics determined a larger proportion of variance for geosmin concentration than TDS in the four table beet genotypes assessed, as reflected in repeatability measurements of 0.90 and 0.43, respectively. This experiment provides support for the primacy of genotype in determining table beet geosmin concentration and a comparatively moderate role of genotype in determining table beet TDS. Thus, genetic manipulation of table beet geosmin could yield cultivars with signature flavor characteristics to serve both niche and mainstream consumer groups, expanding market opportunities for breeders and growers.

scholarly journals RNA-Seq Reveals the Effect of Ethylene on the Volatile Organic Components (VOCs) of Cavendish Banana at Different Post-harvesting Stages

2021 ◽  
Author(s):  
Tongxin Dou ◽  
Chunhua Hu ◽  
Shujing Zhao ◽  
Huijun Gao ◽  
Weidi He ◽  
...  
Keyword(s):  
Solid Phase ◽  
Chemical Properties ◽  
Gas Chromatography Mass Spectrometry ◽  
Rna Seq ◽  
Ripening Stage ◽  
Organic Components ◽  
Cavendish Banana ◽  
Volatile Organic ◽  
Branched Chain ◽  
Post Harvesting

AbstractAroma serves as one of the decisive factors influencing the value of banana commodities. Most of characteristic volatile organic components (VOCs) are formed during post-harvesting. However, the changing of VOCs of banana at different post-harvesting stages remain ambiguous. In this study, the VOCs of Cavendish banana for the four typical post-harvesting stages (green stage/half of yellow stage/yellow ripening stage/over ripening stage) are clarified using headspace solid phase micro-extraction (HS-SPME), combined with gas chromatography-mass spectrometry (GC–MS). The results inferred that the relative content of branched-chain esters such as acetate and butyrate, which form the main contributors of aroma in bananas, is higher in the T2 and T3 stages. Further, RNA-Seq technology was employed to clarify the formation mechanism of banana aroma in the post-harvesting stage. The MaTGL4 gene of the linoleic acid metabolism pathway and the MaBCAT3 and MaBCAT5 genes of the valine, leucine and isoleucine degradation pathway in banana suggest the expression is active late in the ripening stage, and the upregulated expression of these genes is analogous to the formation of aroma components such as branched-chain esters and hexenal. The above results not only provide baseline data on the differences in physical and chemical properties of VOCs in various post-harvesting stages of banana production, but also provide theoretical guidance facilitating the subsequent improvement of the commercial value of bananas through genetic improvement.

scholarly journals Storage behaviour of Impatiens hainanensis seeds stored under three conditions

2021 ◽  
Author(s):  
Weixia Huang ◽  
Yunfang Zhong ◽  
Xinya Meng ◽  
Xiqiang Song ◽  
Mingxun Ren ◽  
...  
Keyword(s):  
Cold Storage ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Storage Period ◽  
Storage Conditions ◽  
Seed Vigour ◽  
Dry Storage ◽  
Antioxidant Enzyme System ◽  
Ambient Storage ◽  
Low Levels

Seeds of Impatiens hainanensis were stored in three different environments: ambient (25°C, 70-80% RH), dry (25°C, 2-5% RH) and cold (4°C, 40-50% RH). The seeds maintained their germination for 90 days in the three storage conditions, albeit at relatively low levels, particularly for seeds in cold storage. Seed vigour was maintained for 45 days for seeds in cold storage, 60 days for seeds in ambient storage, and 90 days for seeds in dry storage. A moisture content around 3.8% is suggested as appropriate for the storage of I. hainanensis seeds. During storage (up to 90 days) under ambient and cold storage conditions, soluble sugar content, SOD and CAT activity all decreased, and were lower than seeds in dry storage. Relative conductivity and TBARS content both increased during ambient and cold storage and were higher than seeds in dry storage after the same storage period. Dry storage better maintained the antioxidant enzyme system of I. hainanensis seeds.

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