scholarly journals Genome-wide Association Analysis Reveals a Novel QTL CsFS1 for Fruit Skin Color in Cucumber

Author(s):  
Hongyu Huang ◽  
Qinqin Yang ◽  
Lidong Zhang ◽  
Weiliang Kong ◽  
Huizhe Wang ◽  
...  

Abstract Fruit skin color is a crucial external trait that affects consumer preference in cucumber. In this study, an F2 population was constructed from a cross between the inbred lines G35 (with light-green fruit skin) and Q51 (with dark-green fruit skin) and used to investigate the inheritance patterns of fruit skin color in cucumber. Genetic analysis showed that dark-green fruit skin was dominant to light-green skin. A major QTL, Fruit skin 1 (CsFS1), was identified between 36.62 Mb and 39.77 Mb on chromosome 3 by BSA-seq and GWAS. We further narrowed down the CsFS1 locus to a 94-kb interval containing 15 candidate genes in three F2 recombinant individuals with light-green skin color and one BC4F2 recombinant individual with dark-green skin color. Among these genes, Csa3G912920, which encodes a GATA transcription factor, was expressed at a higher level in the pericarp of the NIL-1334 line (with light-green fruit skin) than in that of the NIL-1325 line (with dark-green fruit skin). This study provides a novel allele for the improvement of fruit skin color in cucumber breeding.

Genetika ◽  
2013 ◽  
Vol 45 (2) ◽  
pp. 427-432 ◽  
Author(s):  
Emina Mladenovic ◽  
Janos Berenji ◽  
Ksenija Hiel ◽  
Marija Kraljevic-Balalic ◽  
Vladislav Ognjanov ◽  
...  

Bottle gourd [Lagenaria siceraria (Molina) Standl.] is one of the most interesting species in the plant kingdom, due to the diversity of fruit shapes, sizes and ways of use. Warty genotypes are rare compared to non warty genotypes. Considering unusual external appearance of warty fruits, we focused our research on the investigation of its inheritance patterns. By crossing different bottle gourd phenotypes, we studied the mode of inheritance and identified and verified genes responsible for the fruit skin color and warty phenotype segregation. Two parental lines, LAG 70 (with warty fruit of light green color) and LAG 71 (smooth fruit, variegated), F1, F2 and backcrosses populations along with both parents were evaluated. Genetic analysis indicated that warty fruit type is a result of monogenic inheritance, whereby the warty fruit type is dominant (Wt) trait over to the non-warty fruit type (wt). The mode of inheritance of fruit color was controlled by recessive epistasis, with a ratio of 9 variegated (A-, B-), 3 dark green colored (aaB-) and 4 light green colored (aabb) fruits in the F2 generation.


Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2341
Author(s):  
D. S. Kishor ◽  
Hemasundar Alavilli ◽  
Sang-Choon Lee ◽  
Jeong-Gu Kim ◽  
Kihwan Song

Despite various efforts in identifying the genes governing the white immature fruit skin color in cucumber, the genetic basis of the white immature fruit skin color is not well known. In the present study, genetic analysis showed that a recessive gene confers the white immature fruit skin-color phenotype over the light-green color of a Korean slicer cucumber. High-throughput QTL-seq combined with bulked segregation analysis of two pools with the extreme phenotypes (white and light-green fruit skin color) in an F2 population identified two significant genomic regions harboring QTLs for white fruit skin color within the genomic region between 34.1 and 41.67 Mb on chromosome 3, and the genomic region between 12.2 and 12.7 Mb on chromosome 5. Further, nonsynonymous SNPs were identified with a significance of p < 0.05 within the QTL regions, resulting in eight homozygous variants within the QTL region on chromosome 3. SNP marker analysis uncovered the novel missense mutations in Chr3CG52930 and Chr3CG53640 genes and showed consistent results with the phenotype of light-green and white fruit skin-colored F2 plants. These two genes were located 0.5 Mb apart on chromosome 3, which are considered strong candidate genes. Altogether, this study laid a solid foundation for understanding the genetic basis and marker-assisted breeding of immature fruit skin color in cucumber.


2018 ◽  
Vol 2 (2) ◽  
pp. 80
Author(s):  
Helna Estalansa ◽  
Endang Yuniastuti ◽  
Sri Hartati

<p>Breadfruit (Artocarpus altilis) is a plant common are all part of it can be used by humans. The research aims to determine the character of morphological breadfruit and knowing the characterization of morphological breadfruit in Yogyakarta. This research was carried out in June to October 2017 in Sleman Regency (Cangkringan, Ngemplak, Ngaglik), Gunung Kidul (Patuk, Nglipar, Gedangsari), and Bantul (Bantul, Pajangan, Imogiri) in Yogyakarta. Variables are observations include the environmental research and morphology of (trees, leaves, fl owers, and fruits). The data of the morphology are analyzed by NTSYS program. The results showed that the breadfruit trees has a plant height 16,7-17,9 m, the diameter of crown 6,1-8,8 m, shape of crown of the pyramidal, broadly pyramidal, elliptical, irregular, the patterns of branching of erect, opposite, and irregular. The length of leaf 42,2-78,6 cm, width of leaf 30,2-59,2 cm, shape of leaf ovate, and leaf colour dark green. The length of male flowers 9,0-13,4 cm, shape of female flowers globose, shape of male flowers ellipsoid, the colour of female flowers light green, the colour of male flowers light green when young, and brown when old. The length of fruit 12,2 -19,0 cm,diameter of fruit 12,8-16,4 cm, weight of fruit 0,7-1,7 kg, shape of fruit sperical and oblong, fruit skin colour greenish yellow, fruit flesh colour white and light yellow. The diversity of morphology of the breadfruit plants ranging from 3-30%.</p>


2014 ◽  
Vol 139 (6) ◽  
pp. 619-626 ◽  
Author(s):  
Lidia Lozano ◽  
Ignasi Iglesias ◽  
Diego Micheletti ◽  
Michela Troggio ◽  
Satish Kumar ◽  
...  

Single-nucleotide polymorphisms (SNPs) have been used for a range of genetic studies and are now starting to be applied for marker-assisted selection in plant breeding programs. To identify SNP markers associated with red fruit skin color, we conducted a genome-wide association (GWA) analysis in an apple (Malus ×domestica Borkh.) breeding population comprising 94 phenotyped individuals using a 384-plex SNP assay. Linkage disequilibrium (LD) analysis indicated that LD extends over a long physical distance in the population (17 Mbp), indicating that a small number of generations separates the individuals. No significant association of anthocyanin content, overcolor, and colorimetric measures (a*, b*, L*, a/b*, and hue angle) with a marker was identified, although the apple fruit skin color locus has been previously located on apple linkage group 9. Our trial of a small SNP panel for GWA in apple breeding material has demonstrated the limitation of this approach for marker trait association.


HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 602c-602
Author(s):  
Harry S. Paris

Most cultivars of acorn squash (Cucurbita pepo), such as `Table Queen', have fruit that are light green when young, become dark green by intermediate age, and remain dark green through maturity, carrying genotype D/D l-l/l-1 L-2/L-2. Many other forms of C. pepo that carry this genotype, the most familiar being the Halloween and pie pumpkins, turn orange at maturity. The genetic basis for green color retention of acorn squash was investigated by crossing `Table Queen' with `Vegetable Spaghetti', `Fordhook Zucchini', and accession 85k-9-107-2 (the parental, filial, backcross, and testcross generation progenies being grown out in the field and observed and scored for fruit color at maturity, between 40 and 44 days past anthesis). The results indicated that the three stocks crossed with `Table Queen' carry two recessive genes, designated mature orange-1 (mo-1) and mature orange-2 (mo-2), which act in concert to result in complete loss of green color before maturity in 1-1/1-1 plants. `Table Queen' is Mo-l/Mo-1 Mo-2∼o-2. Genes D and mo-2 are linked, ≈15 map units apart.


2016 ◽  
Vol 20 (2) ◽  
pp. 77
Author(s):  
NFN Karsinah ◽  
NFN Rebin ◽  
Lukitariati Sadwiyanti

<p>Evaluation of Mango Hybrids Derived from Crossing of Arumanis-143 with Three Red Mango Cultivars Based on Fruit Characters. Karsinah, Rebin, and Lukitariati Sadwiyanti. In order to improve the character of mango cv. Arumanis-143 that has green-skinned fruit to become red one, Indonesian Tropical Fruits Research Institute Solok had mainactivity, i.e. crossing between Arumanis-143 with Cukurgondang red clones. The objectives of this research were to evaluate the hybrids of mango cv. Arumanis-143 crossing with three red mango cultivars based on fruit characters, and to get 1&amp;ndash;2 new superior varieties candidate that had taste like Arumanis-143 with red/attractive fruit skin color. The research was conducted at Cukurgondang Experimental Field, Pasuruan, East Java from June to December 2011. Plant materials used were four cultivars of parents i.e. Arumanis- 143, Irwin, Gedong Gincu, and Saigon, and 27 hybrids from crossing between Arumanis-143 with Irwin, Arumanis-143 with Gedong Gincu, and Arumanis-143 with Saigon. The results indicated that there were 10 hybrids of 27 hybrids having bear fruits i.e. four hybrids from crossing between Arumanis-143 with Irwin, four hybrids from crossing between Arumanis-143 with Gedong Gincu, and two hybrids from crossing between Arumanis-143 with Saigon. The fruit shape of four hybrids from crossing between Arumanis-143 with Irwin were elliptic, fruits size varies with the smallest fruit weigh was F1-09 i.e. 202 g and the largest was F1-14 i.e. 443 g, fruits taste were sourish sweet-sweet, and the fruits skin color were green-yellow. Fruit shape of four hybrids from crossing between Arumanis-143 with Gedong Gincu were elliptic, fruit size varies with the smallest fruit weigh was F1-87 i.e. 281 g and the largest was F1-18 i.e. 519 g, fruits taste were vapidness sweet-very sweet, the fruits skin color were green-orangish green. The fruit shape of two hybrids from crossing between Arumanis-143 with Saigon were elliptic, fruit weigh of F1-45 was 141 g, fruit taste was sweet with yellowish-red fruit skin color and F1-49 was 232 g, fruit taste was sweet with yellowish green fruit skin color. Result of hibrids evaluation indicated that there was a new superior variety candidate that has taste like Arumanis-143 with yellowish-red fruit skin color.</p><p> </p><p><strong>Abstrak</strong></p><p>Dalam rangka memperbaiki karakter buah mangga Arumanis- 143 yang kulit buahnya berwarna hijau agar menjadi merah, Balai Penelitian Tanaman Buah Tropika, Solok, telah menyilangkan antara mangga Arumanis-143 dengan klon merah Cukurgondang. Penelitian bertujuan untuk mengetahui karakter hibrid hasil persilangan mangga Arumanis-143 dengan tiga kultivar mangga merah berdasarkan karakter buah dan untuk mendapatkan 1&amp;ndash;2 kandidat varietas unggul baru yang mempunyai cita rasa seperti Arumanis-143 dengan kulit buah berwarna merah/menarik. Penelitian dilaksanakan di Kebun Percobaan Cukurgondang, Pasuruan, Jawa Timur, dari bulan Juni sampai Desember 2011. Bahan tanaman yang digunakan ialah empat kultivar tanaman tetua, yaitu Arumanis-143, Irwin, Gedong Gincu, dan Saigon, serta 27 hibrid hasil persilangan Arumanis-143 dengan Irwin, Gedong Gincu, dan Saigon. Hasil penelitian menunjukkan bahwa dari 27 hibrid yang dievaluasi terdapat 10 hibrid yang berbuah, yaitu empat hibrid hasil persilangan antara Arumanis-143 dengan Irwin, empat hibrid hasil persilangan Arumanis-143 dengan Gedong Gincu, dan dua hasil persilangan Arumanis-143 dengan Saigon. Buah dari empat hibrid hasil persilangan antara mangga Arumanis-143 dengan Irwin berbentuk jorong, ukuran buah bervariasi, yang terkecil F1-09 berbobot 202 g dan terbesar F1-14 berbobot 443 g, rasa buah manis asam-manis, kulit buah berwarna hijau-kuning. Buah dari empat hibrid hasil persilangan Arumanis-143 dengan Gedong Gincu berbentuk jorong, ukuran buah bervariasi, yang terkecil F1-87 berbobot 281 g dan terbesar adalah F1-18 berbobot 519 g, rasa buah manis hambar-sangat manis, kulit buah berwarna hijau-hijau orange. Buah dari dua hibrid hasil persilangan Arumanis-143 dengan Saigon berbentuk jorong, buah F1-45 berbobot 141 g, rasa manis dengan kulit buah berwarna merah kekuningan dan buah F1-49 berbobot 232 g, rasa manis dengan kulit buah berwarna hijau kekuningan. Dari hasil evaluasi terhadap hibrid mangga diperoleh satu kandidat varietas unggul baru yang bercita rasa seperti Arumanis-143 dengan kulit buah berwarna merah kekuningan, yaitu F1-45.</p>


2019 ◽  
Vol 14 (2) ◽  
pp. 161
Author(s):  
Dinesh M R ◽  
Sankaran M ◽  
Ravishankar K V ◽  
Sunil Gowda

Varate Giduga is one of the unique variety from Sirsi region of Karnataka. The tree is Semi-circular shaped with dense foliage, leaves about 25cm long; dark green in colour with wavy margin. It is a very consistent and heavy producer. The fruit of this tree matures by mid-April and fruit has a distinctive yellow skin color on fruit exposed to the sun. The fruit shape is round, fruit weight ranged from 350-400 g, fruit length was 9.8 cm, fruit diameter was 9.2 cm, fruit thickness was 8.0cm and bisexual flowers were 16.53%. Biochemical constituents such as the total phenols (317.50 mg/100 g), flavonoids (5.79 mg/100g), carotenoids (1.46 mg/100 g) and acidity (0.19%) were recorded which suggests that these characters are unique as compared to other varieties. The fruit skin is rough and glossy in appearance and the fruit has little or no fiber. It is very delicious in taste with high pulp percentage (74.0%) and high TSS (23.4º B). The flesh color is orange. The fruits have deep orange firm pulp and very sweet with pleasant flavor. Besides,several other traits have drawn special attention to this mango variety as it has large sized fruits (Fig-1), late variety with very good taste, fruit can be cut into two halves by retaining the stone in one half, regular bearer and fruit fly resistant genotype because of its thick peel and high phenolic content in pulp (317.50 mg/100 g).


2015 ◽  
Vol 7 (1) ◽  
pp. 35-39
Author(s):  
Melly Novita ◽  
Satriana Satriana ◽  
Etria Hasmarita

(Lycopen And Carotenoid Contents Of Tomato (Lycopersicum Pyriforme) On Different Maturity Stages: Influence Of Chitosan Coating And Storage).ABSTRACT. Tomato as a young fruit has light green to dark green, hairy, and has a sour taste, bitter, and bad smelling because it contains lycopersicin. However, after its mature became slightly yellow, bright red or dark, yellowish red, yellow or dark red, and it tastes will be good because of more mature the fruit, content of lycopersicin disappeared. Carotenoids especially lycopene and β-carotene is a major component that determines the color of ripe tomatoes. This study was conducted to determine the changes in the content of carotenoids and lycopene along with a change in color in tomatoes were coated with chitosan during storage. The experiment was arranged in completely randomized factorial design (CRD). The first factor was level of maturity (M) that consisted of three (3) levels: M1 = breaker green tomatoes(0-10% red skin), M2 = half-ripe tomatoes (30-60% red skin), M3 = red tomatoes ( 70% red skin). The second factor was storage time (P) consisted of 5 (five) levels: P1 = 0 day, P2 = 5 days, P3 = 10 days = 15 days P4, P5 = 20 days, with 2 replications. Analysis conducted on tomatoes include: analysis of lycopene, carotenoids, and sensory test of skin color. The results showed that the chitosan-coated tomatoes had higher levels of lycopene and carotenoids content than the tomatoes without treatment (control). Level of maturity in tomatoes significantly effect on the formation of lycopene and carotenoids content during storage. In addition to the chitosan coating tomatoes are also able to maintain the color of tomatoes during storage.


Author(s):  
Trương Thị Hồng Hải ◽  
Nguyễn Thị Diệu Thể ◽  
Phan Thu Thảo

In order to establish the pure line of sponge gourd containing aroma feature, we selected the desirable inbred lines by using a self-pollinating method. The present study was investigated to estimate the morphological traits and fruit quality of 6 sponge gourd inbred lines which generated at 4th generation of an aroma Luffa accession B29 under plastic house conditions. The experiment was conducted in a randomized complete block design (RCBD) with three replications, from May to November in 2016. Five plants per replication were examined. The results indicated that all inbred lines could grow well under plastic house conditions. The inbred lines had the same stem and leaf traits; whereas fruit shape, skin color and fruit veins color were observed differently among inbred lines. The aromatic trait was retained in all inbred lines either before or after cooking. The high yield was found in lines BC1 and BC2 by 10.1 tons/ha and 10.7 tons/ha, respectively. These inbred lines should be examined in open field condition to confirm the presence of aromatic trait and yield potential before completion of the procedures for recognition of new Luffa varieties.  


AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chunmiao Jiang ◽  
Gongbo Lv ◽  
Jinxin Ge ◽  
Bin He ◽  
Zhe Zhang ◽  
...  

AbstractGATA transcription factors (TFs) are involved in the regulation of growth processes and various environmental stresses. Although GATA TFs involved in abiotic stress in plants and some fungi have been analyzed, information regarding GATA TFs in Aspergillusoryzae is extremely poor. In this study, we identified and functionally characterized seven GATA proteins from A.oryzae 3.042 genome, including a novel AoSnf5 GATA TF with 20-residue between the Cys-X2-Cys motifs which was found in Aspergillus GATA TFs for the first time. Phylogenetic analysis indicated that these seven A. oryzae GATA TFs could be classified into six subgroups. Analysis of conserved motifs demonstrated that Aspergillus GATA TFs with similar motif compositions clustered in one subgroup, suggesting that they might possess similar genetic functions, further confirming the accuracy of the phylogenetic relationship. Furthermore, the expression patterns of seven A.oryzae GATA TFs under temperature and salt stresses indicated that A. oryzae GATA TFs were mainly responsive to high temperature and high salt stress. The protein–protein interaction network of A.oryzae GATA TFs revealed certain potentially interacting proteins. The comprehensive analysis of A. oryzae GATA TFs will be beneficial for understanding their biological function and evolutionary features and provide an important starting point to further understand the role of GATA TFs in the regulation of distinct environmental conditions in A.oryzae.


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