Role of Osteoprotegerin as Novel Marker in Urinary Stone Formers: A Pilot Study

Background: Urinary tract calculus formation can be prevented by identifying molecules and metabolic disturbances that affect this process. Osteoprotegerin (OPG), a cytokine of the TNF receptor superfamily, has been demonstrated to mediate vascular calcification and intimal calcification. Endothelial injury and oxidative stress are known to play a role in urolithiasis in the form of Rendall’s plaques. Objectives: The present study aimed to compare 24-h urinary and serum OPG levels of patients with and without urolithiasis. Methods: In this case-control study, 24-h urinary levels of OPG (pg/mL), serum levels of OPG (pg/mL), and creatinine (mg/dL) were measured in both groups. Urinary and serum levels of OPG were determined by enzyme-linked immunosorbent assay (ELISA) using human OPG kits. Results: Mean serum creatinine was 0.86 ± 0.21 mg/dL in the case group and 0.77± 0.16 mg/dL in controls. The difference in the mean serum OPG levels between the cases (227.13 ± 98.02 pg/mL) and controls (47.28 ± 29.61 pg/mL) was highly significant (P value < 0.0001). The difference in the mean 24-h urinary OPG levels between the cases (156.12 ± 174.31 pg/mL) and controls (9.32 ± 23.72 pg/mL) was highly significant (P value < 0.001). Conclusions: There were significantly higher levels of OPG in serum and 24-h urine samples of cases than in controls. Hence, it requires further large studies to make OPG a diagnostic and prognostic marker.

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Urine CA-2 as a biomarker for diagnosis urinary stone and prediction of its complications

10.21203/rs.3.rs-59394/v1 ◽

2020 ◽

Author(s):

Yuan-jing Leng ◽

Hai-bin Zhou ◽

Jiang-ling Fu ◽

Wen-juan Wang

Keyword(s):

Healthy Subjects ◽

Characteristic Curve ◽

Urinary Stone ◽

Urinary Stones ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Healthy Control ◽

The Mean ◽

The Difference ◽

The Relationship

Abstract PURPOSECarbonic anhydrase-2 (CA-2) plays a role in mineralization and calcification in organism. Strong evidence suggests that CA-2 is associated with urolithiasis. However, the relationship between CA-2 and urinary stone remains unclear. The study aimed to assess the association of urine CA-2 (uCA-2) level and the potential risk of urinary stone.METHODSFrom March 2017 to November 2019, a prospective cohort study was conducted on patients with urinary stones and healthy subjects to determine the pretreatment uCA-2 level detection by Enzyme linked immunosorbent assay (ELISA). The difference of uCA-2 level between patients with urinary stones and healthy subjects was compared. Then comparison between stone patients with complications and without complications was carried out as well as correlation analysis to detect factors associated with biomarker expression.RESULTS118 patients with urinary stones were into urinary stones group and 42 healthy subjects were into healthy control group. The mean pretreatment uCA-2 level was significantly higher in patients with urinary stones group than healthy controls group (P=0.028). Furthermore, The uCA-2 level was positive correlation with urinary stones complications (R=0.379, P=0.000), especially pain complications (R=0.524, P=0.000) and hematuria complications (R=0.374, P=0.000). Receiver operating characteristic curve (ROC) analysis that a uCA-2 level threshold of 10.94 ng/mL had 83.67% sensitivity and 68.12% specificity for predicting urinary stones complications. CONCLUSIONSExcessive uCA-2 excretion is a major risk factor for urinary stone. Our findings suggested that uCA-2 may be used as an unappreciated biomarker for the diagnosis urinary stone in patients and to predict its complications.

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Evaluation of Clinical Symptoms and Serum Lead Level in Patients with History of Oral Opium Use being Admitted to Hazrat Rasoul Akram, Firoozgar, Firoozabadi and Haft Tir Hospitals, Tehran

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2019/v31i630344 ◽

2019 ◽

pp. 1-8

Author(s):

Majid Rezaei Tavirani ◽

Hazhir Heidari Beigvand

Keyword(s):

Clinical Symptoms ◽

Middle Eastern ◽

Lead Level ◽

Serum Levels ◽

Control Group ◽

Case Group ◽

History Of ◽

The Mean ◽

The Difference ◽

Opiate Addicts

Introduction: Drug abuse and its complications is a socio-health problem in Middle Eastern countries such as Iran. Smugglers may add lead to drug during drug production, which is in order to increase its weight for greater benefit. Considering the frequency of the patients with various complaints among Iranians, this study was designed and conducted to evaluate the clinical symptoms and serum levels of lead in patients being admitted to Hazrat Rasoul Akram, Firoozgar, Firouzabadi and Haft Tir hospitals with any complaints. Materials and Methods: In this case-control study, serum levels of lead were measured in 128 samples in case and control groups. The case group consisted of 64 patients using oral opium who being admitted to Rasoul Akram and Firoozgar, Haft Tir and Firouzabadi hospitals with different complaints in May 2017. The control group consisted of 64 patients with no history of addiction that were homogenized with the case group in terms of age and sex. They were evaluated for serum levels of lead and other variables. Data were analyzed by SPSS software. Results: The mean serum lead level was 76.34±17.82 in the group using opium and was 7.68±3.72 in the control group that the difference was statistically significant (P <0.001). The most common complaints of patients were abdominal pain and symptoms of bowel obstruction. The mean rate of oral opium consumption was 1.73±0.23 in subjects under 50 years old and with a mean of 2.89±0.27 in subjects over 50 years old. Serum lead level was significantly (P = 0.032) increased compared to the amount consumed. Duration of oral opium consumption was 5 months to 30 years with a mean of 15.24 years, which was not significantly correlated to serum lead level (P = 0.213). Also, the hemoglobin range was significantly correlated to different levels of lead in patients consuming oral opium (P = 0.027). Conclusion: The findings of the present study showed a high mean serum lead level in oral opium addicts in the study population. The results also confirm numerous reports suggesting the definitive diagnosis of lead poisoning as a justifying factor in addicted patients with nonspecific symptoms, which may indicate the need for serum lead level screening in opiate addicts to prevent more serious complications.

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Role of Cardiac Biomarkers in the Assessment of Acute Cerebrovascular Accident

Journal of Neurosciences in Rural Practice ◽

10.1055/s-0040-1721198 ◽

2021 ◽

Vol 12 (01) ◽

pp. 106-111

Author(s):

Srinivasan Radhakrishnan ◽

Swathy Moorthy ◽

Sudish Gadde ◽

Krishnaswamy Madhavan

Keyword(s):

Acute Stroke ◽

Cerebrovascular Accident ◽

Troponin I ◽

Serum Levels ◽

Assay Method ◽

Enzyme Linked Immunosorbent Assay ◽

Cardiac Enzymes ◽

Mean Values ◽

Creatine Kinase Mb ◽

The Mean

Abstract Background Stroke (cerebrovascular accident) has for long been a global burden in terms of its morbidity and mortality. Serum levels of cardiac enzymes such as creatine kinase-MB (CK-MB) component, troponin T, and brain natriuretic peptide have been found to be elevated among the patients with stroke and also serve to prognosticate these patients. The serum levels of these enzymes correlate directly to the severity of stroke in these patients. Objective Elevated cardiac enzymes among patients with acute cerebrovascular accidents are not uncommon despite the patients not having any cardiac problems. We aimed to identify the occurrence of elevated cardiac enzymes among patients with acute stroke and their correlation with the severity of stroke. Materials and Methods Our study included 100 patients of acute stroke with no previous history of cardiac ailments. Serum levels of troponin I and CK-MB were analyzed among these patients using enzyme-linked immunosorbent assay method within the first 2 hours of admission. Patients outcome during the hospital stay were analyzed. Stroke severity was assessed using the National Institute of Health Stroke score (NIHS score) and the modified Rankin Score (mRS). The cardiac enzyme levels were correlated with these scores. Results Twenty-eight percent of patients had elevated troponin I, while 72% patients had normal levels with the mean values of 10.36 to 106.54 ng/mL and 0.00 to 0.02 ng/mL, respectively. CK-MB levels were found elevated among 14% patients and normal among 86% patients with mean values of 5.8 to 124.36 and 0.0 to 4.3 ng/mL, respectively. Among the six patients who succumbed to death, three patients had increased troponin I and four had elevated CK-MB. NIHS scores of 21.0357±6.79 and 105.277±5.564 were seen in patients with elevated and normal troponin I, whereas NIHS scores of 20.4285±8.658 and 11.8721±9.273 were seen among patients with increased and normal CK-MB, respectively. The mRS scores were 4.3214±0.367, 2.4305±1.374, 4.2143±1.412, and 2.756±1.749 ng/mL among the patients with elevated and normal troponin I and CK-MB, respectively. Conclusion The mean values of cardiac enzymes troponin I and CK-MB were higher among patients with higher scores of NIHS and mRS. Among them, troponin I was very significant and it may serve as an early biomarker for the severity of stroke and hint on early cardiac evaluation among these patients.

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Cross-sectional study to compare the biochemical parameters in low normal versus high normal plasma glucose levels in euglycemic individuals

International Journal of Advances in Medicine ◽

10.18203/2349-3933.ijam20202117 ◽

2020 ◽

Vol 7 (6) ◽

pp. 997

Author(s):

Dharmendra Jhavar ◽

Neha Kirti ◽

Sumit Kumar Vishwakarma ◽

Umesh Kumar Chandra ◽

Vinod Verma

Keyword(s):

Uric Acid ◽

Total Cholesterol ◽

General Medicine ◽

Cross Sectional Study ◽

Normal Group ◽

P Value ◽

Sectional Study ◽

Cross Sectional ◽

The Mean ◽

The Difference

Background: Since a long time ago, the experts have realized that determination of cut-off point for diagnosing diabetes will be revised over time with the lower blood glucose level as the more sensitive diagnosis for detecting the occurring complication and biochemical changes.Methods: This cross sectional study was carried out in the department of medicine, M.G.M. Medical College and M.Y. Hospital Indore from July, 2016 to August, 2017 in 200 individuals and patients having euglycemic status attending General Medicine OPD.Results: In the low and high normal group 2 (2.0%) and 8 (8.0%) were having abnormal total cholesterol (TC) level respectively. The mean total cholesterol in the low normal group was 117.16±26.94mg/dl and it was 154.74±28.38mg/dl in the high normal group. The difference was found to be statistically significant (p value 0.000). In the low and high normal group, 4 (4.0%) and 17 (17.0%) were having abnormal triglyceride (TG) levels respectively. The mean TG levels in the low and high normal group were 96.93±22.64mg/dl and 110.55±32.37mg/dl respectively. The difference was found to be statistically significant (p value 0.001). In the low and high normal group, 6 (6.0%) and 14 (14.0%) patient was having abnormal uric acid levels respectively. The mean uric acid levels in the low and high normal group was 4.88±1.10mg/dl and 5.31±1.31mg/dl respectively. The difference was found to be statistically significant (p value 0.013).Conclusions: Higher levels of Cholesterol and Triglycerides were found more commonly in high normal euglycemic group compared to low normal euglycemic group. Mean cholesterol and mean triglyceride levels were higher in high normal euglycemic group.

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Serum CYR61 As A Potential Biomarker for The Diagnosis of Esophagogastric Junction Tumor

Bioscience Reports ◽

10.1042/bsr20204117 ◽

2021 ◽

Author(s):

Ling-Yu Chu ◽

Jian-Yuan Zhou ◽

Yi-Xuan Zhao ◽

Yan-Ting Ou ◽

Tian Yang ◽

...

Keyword(s):

Early Stage ◽

Area Under The Curve ◽

Serum Levels ◽

Enzyme Linked Immunosorbent Assay ◽

Operating Characteristics ◽

Tumor Patients ◽

Chi Square ◽

Potential Biomarker ◽

The Difference ◽

Normal Controls

Background：Esophagogastric junction tumor (EGJ) is a rare but fatal disease with a rapid rising incidence worldwide in the late 20 years, and it lacks a convenient and safe method for diagnosis. This study aimed to evaluate the potential of serum CYR61 as a biomarker for the diagnosis of EGJ tumor. Methods: Enzyme-linked immunosorbent assay (ELISA) was used to estimate CYR61 levels in sera of 152 EGJ tumor patients and 137 normal controls. Receiver operating characteristics (ROC) was carried out to evaluate the diagnostic accuracy. The Mann–Whitney’s U test was used to compare the difference of serum levels of CYR61 between groups. And chi-square tests were employed to estimate the correlation of the positive rate of serum CYR61 between/among subgroups. Results: Serum CYR61 levels were statistically lower in EGJ tumor and early-stage EGJ tumor patients than those in normal controls (P<0.0001). The sensitivity, specificity, and the area under the curve (AUC) of this biomarker in EGJ tumor were 88.2%, 43.8% and 0.691, respectively, and those for early stage of EGJ tumor were 80.0%, 66.4% and 0.722, respectively. Analyses showed that there was no correlation between the clinical data and the levels of CYR61 (P>0.05). Conclusion: This study showed that CYR61 might be a potential biomarker to assist the diagnosis of EGJ tumor.

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Getting Vaccinated Helps: Prospective Study Reveals Lower CT Severity Scores amongst COVID Vaccine Recipients

Indian Journal of Radiology and Imaging ◽

10.1055/s-0041-1741052 ◽

2022 ◽

Author(s):

Priscilla C. Joshi ◽

Vandana Jahanvi ◽

Mangal S. Mahajan ◽

Nivedita C. Ghule Patil ◽

Priyankkumar G. Moradiya ◽

...

Keyword(s):

Disease Severity ◽

Age Groups ◽

P Value ◽

Disease Prognosis ◽

Important Message ◽

Severity Scores ◽

Reduce Infection Rate ◽

The Mean ◽

The Difference ◽

The Government

Abstract Context Computerized tomography (CT) is an invaluable imaging investigation for evaluating COVID-19 disease. CT detects early changes of COVID-19 pneumonia and predicts the disease prognosis based on a semiquantitative 25-point CT severity score (CT-SS). India launched its vaccination drive in January 2021 with two different vaccines being approved by the government. These vaccines are believed to prevent the disease itself, in majority of the cases and at least decrease disease severity, in the rest. Aim This study aims to evaluate the CT-SS in vaccinated and non-vaccinated subjects who have been diagnosed with COVID-pneumonia or are COVID suspects. Subjects and Methods A total of 3,235 patients with typical COVID-19 related imaging findings on HRCT thorax were included in the study. These subjects were divided into three age categories, 18–44, 45–59 and ≥60 years. The CT severity scores were allotted by experienced radiologists. Medians of the scores in different age groups were compared amongst vaccinated and non-vaccinated individuals using the Kruskal–Wallis H test. A p-value < 0.05 was considered significant. All results were shown with 95% confidence interval. Results The difference in the medians amongst the vaccinated and non-vaccinated groups was significant, p-values being < 0.001 in all age categories. Conclusion The mean CT-SS was less in vaccinated subjects and the difference in median CT-SS amongst vaccinated and non-vaccinated individuals was statistically significant, thus sending an important message that it is mandatory for the population at large to get vaccinated to reduce infection rate/disease severity.

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Study of outcome of the treatment with intravenous iron sucrose in moderately anaemic pregnant women

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20193695 ◽

2019 ◽

Vol 8 (9) ◽

pp. 3480

Author(s):

Kohila Kalimuthu ◽

Vanusha Avudaithangam

Keyword(s):

Standard Deviation ◽

Iron Deficiency ◽

Pregnant Women ◽

Iron Deficiency Anaemia ◽

Intravenous Iron ◽

Iron Sucrose ◽

P Value ◽

Deficiency Anaemia ◽

The Mean ◽

The Difference

Background: Moderate anaemia seen in about 15-20% of pregnant women. Iron sucrose complex which is used intravenously for the correction of Iron deficiency anaemia. The drug has been able to raise the haemoglobin to satisfactory level when used in moderately anaemic iron deficient pregnant women. The objective of this study was to study the improvement of Hb% after treatment with intravenous Iron sucrose complex in moderately anaemic pregnant women belonging to 24-32 weeks of gestational age.Methods: 50 antenatal patients between gestational age 24-32 weeks with hemoglobin between 8-9.5g/dl were selected and included in this study. They were subjected to blood hemoglobin estimation, hematocrit and peripheral smear study. In each infusion, the maximum total dose administered was 200 mg iron sucrose in 100 ml of normal saline, slow IV infused over 30 minutes. Monitoring was done throughout the infusion to observe for any side effects.Results: Mean hemoglobin among the 50 patients before starting the therapy was 8.172g/dl and the mean hemoglobin at the end of one month of completing the therapy was 11.066g/dl. The rise in mean hemoglobin i.e. the difference in the mean hemoglobin before and after treatment was 2.894g/dl. The p value is 0.0001 which is statistically significant. The mean hematocrit of the 50 patients studied before starting the treatment was 26.772% with a standard deviation of 1.914. The mean hematocrit after completing the therapy was 33.872% with a standard deviation of 1.321. The difference in the mean hematocrit was 7.100% with a p value of 0.0001 which is statistically significant.Conclusions: Intravenous iron sucrose complex is well tolerated and highly efficacious in improving hemoglobin, hematocrit in the treatment of iron deficiency anaemia in antenatal women.

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Persepsi Petani terhadap Pilihan Menjual Padi Kepada Penebas atau Perpadi (Kasus Subak Benel, Desa Kaliakah, Kecamatan Negara, Kabupaten Jembrana)

Jurnal Agribisnis dan Agrowisata (Journal of Agribusiness and Agritourism) ◽

10.24843/jaa.2017.v06.i04.p11 ◽

2017 ◽

pp. 563

Author(s):

I MADE PRASADA ARY WIRAWAN ◽

I DEWA PUTU OKA SUARDI ◽

I MADE SARJANA

Keyword(s):

T Test ◽

Mean Difference ◽

P Value ◽

Food Crops ◽

Test Program ◽

Qualitative Descriptive ◽

The Mean ◽

The Difference ◽

The Government ◽

Better Than

Farmers Perception Of Options To Sell Rice Penebas Or Perpadi(especially in Subak Benel, Kaliakah Village, Negara districts,Jembrana Regency) Various efforts have been implemented by the Provincial Food Crops Agency of Balito stabilize the price of grain, this is done by giving Funds of Rural EconomicEmpowerment Capital to the government for the purchase of rice. Although it hasbeen done from 2003, farmers are still selling it to Penebas with a bondage system.The purpose of this study to determine the perception of farmers to the choice ofselling rice to Perpadi or Penebas seen and differences in farmers' perceptions of thechoice of selling rice to Penebas or Perpadi. The research location is located in SubakBenel, Kaliakah Village, State District, Jembrana District. The analytical methodused is qualitative descriptive that aided with score, to answer the purpose ofresearch by using questionnaire. The results of this study indicate that the perceptionof farmers to the choice of selling rice to Perpadi better than to Penebas. This is seenin the achievement of the farmers' choice of selling paddy to Perpadi is very goodand to Penebas is good. The difference of Perpadi with Penebas is measured throughDifferent Test with SPSS Independent Sample T test program which the result of Sigvalue. Or p value of 0.004 where <0.05 then there is a statistically significantdifference in probability 0.05, the mean difference or mean of both groups is shownin Mean Difference ie .29559.

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An Evaluation of the Correlation between Hepcidin Serum Levels and Disease Activity in Inflammatory Bowel Disease

Gastroenterology Research and Practice ◽

10.1155/2015/810942 ◽

2015 ◽

Vol 2015 ◽

pp. 1-4 ◽

Cited By ~ 11

Author(s):

Zehra Betül Paköz ◽

Cem Çekiç ◽

Mahmut Arabul ◽

Elif Sarıtaş Yüksel ◽

Serkan İpek ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Disease Activity ◽

Bowel Disease ◽

Active Phase ◽

Serum Levels ◽

Enzyme Linked Immunosorbent Assay ◽

C Reactive Protein ◽

Reactive Protein ◽

Inflammatory Bowel ◽

The Mean

Aim. While there are many well-defined serological markers for inflammatory bowel disease (IBD), there is limited evidence that they positively affect clinical outcomes. This study aimed to evaluate the correlation between hepcidin serum levels and disease activity in IBD.Materials and Methods. Eighty-five consecutive IBD patients were enrolled in the study. Hepcidin serum levels were assessed using an enzyme-linked immunosorbent assay (ELISA) and were compared with disease activity as well as the interleukin-6 (IL-6) and C-reactive protein (CRP) levels.Results. The mean hepcidin serum levels in Crohn’s disease (CD) patients in remission and in the active phase were3837±1436and3752±1274 pg/mL, respectivelyP=0.613. The mean hepcidin serum levels in ulcerative colitis (UC) patients in remission and in the active phase were4285±8623and3727±1176 pg/mL, respectivelyP=0.241. Correlation analysis between inflammatory markers and hepcidin serum levels indicated that there was no correlation between hepcidin levels and IL-6P=0.582or CRPP=0.783.Conclusion. As an acute-phase protein, hepcidin seems to have a lower efficacy than other parameters in the detection of activation in IBD.

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Biochemical urinalysis of healthy kidney and stone-generating kidney in unilateral urolithiasis

Journal of Renal Injury Prevention ◽

10.15171/jrip.2019.28 ◽

2019 ◽

Vol 8 (2) ◽

pp. 151-156

Author(s):

Feramarz Mohammadalibeigi ◽

Majid Shirani ◽

Hamed Seyed-Salehi ◽

Lotfollah Afzali

Keyword(s):

Uric Acid ◽

Kidney Stone ◽

Stone Formation ◽

Urinary Stone ◽

Metabolic Factors ◽

Cross Sectional ◽

Urinary Sample ◽

The Mean ◽

The Difference ◽

Southwest Iran

Introduction: Oxalate, calcium, uric acid, and citrate are among the most widely known biochemical factors for urinary stone formation. In most cases, urolithiasis occurs as unilateral despite the role of systemic metabolic factors in kidney stone formation. Objectives: The present research aimed to compare these urinary biochemical factors in healthy and stone-generating kidneys in patients with unilateral urolithiasis. Patients and Methods: Forty patients with unilateral urolithiasis participated in this cross-sectional, descriptive-analytical study. The patients were hospitalized in kidney surgery and kidney urology ward of Kashani hospital of Shahrekord, southwest Iran. After the implementation of crushing stone using transurethral lithotripsy (TUL), 5-10 cc of urinary sample was collected from each kidney. An AutoAnalyzer (Mindray Company, Bs–360 model) and laboratory kits (Bionic Company) were used to measure calcium, uric acid, and creatinine. Moreover, a manual method and Darman Faraz Kave company kits were applied to measure the levels of oxalate and citrate. Results on the healthy and stone-generating kidneys of each patient were separately analyzed using the Stata 13 software. Results: The difference in the mean ratio of uric acid, oxalate, and citrate to creatinine in the healthy kidneys and stone-generating kidneys was not significant. However, the mean UCa/UCr ratio in the healthy kidneys was 0.27±0.07 and relatively greater than that in the stone-generating kidneys (0.11±0.04) (P=0.06). Conclusion: The studied topical factors and secretory disorders had not any significant relationship with unilateral urolithiasis. The cause of unilateral urolithiasis should be searched in other factors such as metabolic factors, main positions of individual during 24 hours and sleeping, and anatomic disorders of kidney stone, or multifactorial.

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