Adropin and Irisin Deficiencies Are Associated With Presence of Diagonal Earlobe Crease in CAD Patients

Background and Aims: Diagonal earlobe crease (ELC) has been considered a potential cutaneous marker of atherosclerosis. However, the potential mechanism by which ELC and atherosclerosis are linked has not been adequately defined. Roles of adropin and irisin, novel biomarkers of endothelial function, in ELC have not been well-studied. This study aimed to test whether individuals with ELC are deficient in adropin and irisin, a characteristic that would likely promote endothelial dysfunction and provide a plausible common pathological basis for atherosclerosis and ELC.Methods: Patients diagnosed with coronary artery disease (CAD) with (n = 45) and without (n = 45) ELC were consecutively enrolled in the study. The ages of the patients enrolled ranged from 40–70 years. Other patients (n = 45) without ELC or CAD were recruited as the control group. All patients underwent coronary angiography. Serum adropin and irisin concentrations were assessed via enzyme-linked immunosorbent assay.Results: Circulating levels of irisin in the ELC group were significantly lower than those in the non-ELC group, and were highest in the control group. Serum adropin levels of the ELC group were significantly lower than those of the non-ELC group (P < 0.001). Interestingly, although the serum adropin level of the control group was greater than that of the non-ELC group, the difference failed to achieve statistical significance. In subgroup analysis of CAD and ELC, both serum adropin and irisin levels of the CAD and ELC groups were lower than those of the control group (P < 0.001). Receiver-operating characteristic curve analysis revealed that adropin and irisin have similar prognostic power for CAD and ELC.Conclusions: Low adropin and irisin were significantly associated with CAD and ELC. The deficiencies in adropin and irisin may be a common cause of both atherosclerosis and ELC, which explains why patients with ELC are prone to CAD.

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Urine CA-2 as a biomarker for diagnosis urinary stone and prediction of its complications

10.21203/rs.3.rs-59394/v1 ◽

2020 ◽

Author(s):

Yuan-jing Leng ◽

Hai-bin Zhou ◽

Jiang-ling Fu ◽

Wen-juan Wang

Keyword(s):

Healthy Subjects ◽

Characteristic Curve ◽

Urinary Stone ◽

Urinary Stones ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Healthy Control ◽

The Mean ◽

The Difference ◽

The Relationship

Abstract PURPOSECarbonic anhydrase-2 (CA-2) plays a role in mineralization and calcification in organism. Strong evidence suggests that CA-2 is associated with urolithiasis. However, the relationship between CA-2 and urinary stone remains unclear. The study aimed to assess the association of urine CA-2 (uCA-2) level and the potential risk of urinary stone.METHODSFrom March 2017 to November 2019, a prospective cohort study was conducted on patients with urinary stones and healthy subjects to determine the pretreatment uCA-2 level detection by Enzyme linked immunosorbent assay (ELISA). The difference of uCA-2 level between patients with urinary stones and healthy subjects was compared. Then comparison between stone patients with complications and without complications was carried out as well as correlation analysis to detect factors associated with biomarker expression.RESULTS118 patients with urinary stones were into urinary stones group and 42 healthy subjects were into healthy control group. The mean pretreatment uCA-2 level was significantly higher in patients with urinary stones group than healthy controls group (P=0.028). Furthermore, The uCA-2 level was positive correlation with urinary stones complications (R=0.379, P=0.000), especially pain complications (R=0.524, P=0.000) and hematuria complications (R=0.374, P=0.000). Receiver operating characteristic curve (ROC) analysis that a uCA-2 level threshold of 10.94 ng/mL had 83.67% sensitivity and 68.12% specificity for predicting urinary stones complications. CONCLUSIONSExcessive uCA-2 excretion is a major risk factor for urinary stone. Our findings suggested that uCA-2 may be used as an unappreciated biomarker for the diagnosis urinary stone in patients and to predict its complications.

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CORRELATION BETWEEN SERUM CALCIUM LEVELS AND SMEAR GRADING AMONG THE PULMONARY TUBERCULOSIS PATIENTS

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v3i3.158 ◽

2019 ◽

Vol 3 (3) ◽

Author(s):

Pramod Sood ◽

Sanjay Bhat

Keyword(s):

Pulmonary Tuberculosis ◽

Causes Of Death ◽

Statistical Significance ◽

Control Group ◽

Healthy Individuals ◽

Tuberculosis Patients ◽

Cause Of Deaths ◽

The Mean ◽

The Difference ◽

Group Serum

Background: Tuberculosis (TB) is contagious and airborne. TB was one of the top 10 causes of death worldwide in 2017. It is also the leading killer of people with HIV and a major cause of deaths related to antimicrobial resistance. Methods: Microscopically confirmed TB patients were included in the cases group and healthy individuals in the control group. Serum Ca was estimated by kit method, by auto analyzer. ANOVA and t- test was used to find the statistical significance. Results: The mean serum Ca was 7.32±1.22 mg/dl in TB and 9.41±2.13 mg/dl in controls. The mean serum Ca levels were 7.22±1.20 mg/dl, 7.14±1.14 mg/dl, 7.12±1.10 mg/dl and 7.06±1.02 mg/dl respectively for scanty, 1+. 2+ and 3+; statistically the difference were not significant (P > 0.05) between the grades. Conclusion: Hypocalcemia observed in pulmonary tuberculosis patients. No correlation was found between the mean serum Ca levels and grading. Keywords: Calcium, TB, AFB.

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Clinical Study of Qingpeng Ointment Treatment of Shoulder-Hand Syndrome After Cerebral Hemorrhage During the Rehabilitation Period

Combinatorial Chemistry & High Throughput Screening ◽

10.2174/1386207323666201211093227 ◽

2020 ◽

Vol 23 ◽

Author(s):

Ruihuan Pan ◽

Shanshan Ling ◽

Haodong Yang ◽

Yan Huang ◽

Lechang Zhan ◽

...

Keyword(s):

Treatment Group ◽

Cerebral Hemorrhage ◽

Clinical Medicine ◽

External Rotation ◽

Statistical Significance ◽

Hand Function ◽

Tibetan Medicine ◽

Controlled Study ◽

Control Group ◽

The Difference

Background: Shoulder-hand syndrome (SHS) refers to a syndrome causing sudden edema, shoulder pain and limited hand function. Qingpeng ointment, a kind of Tibetan medicine, can reduce swelling, relieve pain, tonify stagnation and clear the meridians, which is consistent with the pathological mechanism of SHS after stroke. Therefore, if clinical trials can be used to explore the effectiveness of Qingpeng ointment for treatment of poststroke SHS and promote its application in clinical medicine, this is of specific significance for the treatment of poststroke SHS. Objective: To investigate the clinical efficacy and safety of Qingpeng ointment in the treatment of poststroke SHS. To provide an objective basis for a better therapeutic treatment for poststroke SHS． Method: A prospective, randomized, controlled study was conducted. This study recruited 120 patients with poststroke SHS who met the inclusion criteria. They were randomized into the treatment group and the control group, with 60 patients allocated to each group. The treatment group received routine medical treatment and rehabilitative care after using the Qingpeng ointment, while the patients in the control group received only routine treatment without the ointment. All patients received clinical assessment with the Visual Analogue Scale (VAS), measurement of the range of motion (ROM) of the upper-limb joints, the Fugl-Meyer Assessment of Upper Extremity (FMA-U) and the Modified Barthel Index Score (MBI) before and after the whole treatment. Results: After 4 weeks of treatment, the VAS scores of both groups were decreased significantly (P＜0.05), and the difference between the two groups was statistically significant (P < 0.05). There is no statistical significance for the difference between the treatment group and control group in terms of the FMA-U and MBI scores and the forward bend, backward, outstretch, external rotation and pronation angles after treatment. The increases in the values of VAS, FMA-M and MBI in the treatment group were greater than those in the control group, and the difference was statistically significant (P < 0.05). The increases in the values of the forward bend, outreach and external rotation angles in the treatment group were greater than those in the control group, and the difference was statistically significant (P < 0.05). Conclusion: The treatment group showed better results than the control group in terms of the relief of pain symptoms, the improvement of motor function and the improvement of the activities of daily living for patients with shoulder-hand syndrome after cerebral hemorrhage. Qingpeng ointment is effective and safe in treating poststroke SHS.

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Involvement of the Inconstant Bursa of the Fifth Metatarsophalangeal Joint in Psoriatic Arthritis: A Clinical and Ultrasonographic Study

BioMed Research International ◽

10.1155/2014/174841 ◽

2014 ◽

Vol 2014 ◽

pp. 1-5 ◽

Cited By ~ 1

Author(s):

Giovanni Ciancio ◽

Stefania Volpinari ◽

Maria Fotinidi ◽

Federica Furini ◽

Ilaria Farina ◽

...

Keyword(s):

Psoriatic Arthritis ◽

Healthy Subjects ◽

Statistical Significance ◽

Metatarsophalangeal Joint ◽

Metatarsal Bone ◽

The Other ◽

Control Group ◽

Asymptomatic Patients ◽

Seronegative Spondyloarthritis ◽

The Difference

Objective. To evaluate the involvement of the bursa located next to the head of the 5th metatarsal bone in patients with psoriatic arthritis (PsA) in comparison with the other seronegative spondyloarthritis (SpA).Methods. All patients with PsA seen during a period of 24 months were enrolled. The control group included healthy subjects and patients with the other SpA. All subjects underwent clinical and ultrasound (US) examination of the lateral surface of the 5th metatarsal.Results. 150 PsA patients (88 M; 62 F), 172 SpA (107 M; 65 F), and 95 healthy controls (58 M; 37 F) were evaluated. Based on clinical and US evaluation, bursitis was diagnosed in 17/150 (11.3%) PsA patients but in none of the SpA (P<0.0001) and healthy (P=0.0002) controls. In detecting bursitis, US was more sensitive than clinical examination, although the difference did not reach statistical significance (P=0.09).Conclusion. The bursa of the 5th metatarsophalangeal joint appears to be involved in PsA more frequently than by chance. If confirmed by other studies, this finding could be considered as a distinctive clinical sign of PsA, useful for differential diagnosis with the other SpA. In asymptomatic patients, US proved to be more sensitive in the detection of bursitis.

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Autoimmune thyroid disease in patients with anti-GAD positive type 1 diabetes mellitus

Open Medicine ◽

10.2478/s11536-009-0080-z ◽

2009 ◽

Vol 4 (4) ◽

pp. 415-422

Author(s):

Kamile Gul ◽

Ihsan Ustun ◽

Yusuf Aydin ◽

Dilek Berker ◽

Halil Erol ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 1 Diabetes ◽

Type 1 Diabetes Mellitus ◽

Statistical Significance ◽

Control Group ◽

Acid Decarboxylase ◽

Thyroid Peroxidase ◽

The Difference ◽

Type 1 Dm

AbstractThe aim of the study was to determine the frequency and titers of anti-thyroid peroxidase (Anti-TPO), anti-thyroglobulin (Anti-TG), and anti-glutamic acid decarboxylase (Anti-GAD) antibodies in Turkish patients with type 1 diabetes mellitus (DM), and to compare the frequency of anti-TPO and anti-TG titers in the presence or absence of anti-GAD. A total of 104 patients including 56 males and 48 females with type 1 DM and their age-, gender-, and body mass index-matched control group, including 31 males and 27 females, 58 cases in total with an age range of 15-50 years, were recruited into this study. In patients with type 1 DM, positive anti-GAD was detected in 30.8% (n=32). In patients with positive anti-GAD, rate of positive anti-TPO was 37.5%; however, in patients with negative anti-GAD, the rate of positive anti-TPO was 9.7% and the difference was statistically significant (p=0.001). In patients with positive anti-GAD, the rate of positive anti-TG was 18.8%. In patients with negative anti-GAD, the rate of positive anti-TG was 2.8%, and the difference between them was statistically significant (p=0.005). In patients with positive and negative anti-GAD, rates of both positive anti-TPO and anti-TG were 15.6% and 1.4%, respectively, with the difference showing statistical significance (p=0.004). Thyroid autoimmunity in type 1 DM patients with positive anti-GAD was apparently higher; therefore, these patients should be followed more frequently and carefully.

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Patients with New-Onset Tumor of Severe Coronary Artery Disease May be at a Higher Risk of Arrhythmia

Cardiology Research and Practice ◽

10.1155/2021/1948624 ◽

2021 ◽

Vol 2021 ◽

pp. 1-4

Author(s):

Palisha Alimu ◽

Dezhi Yang ◽

Huatao Zhou ◽

Yan Wu ◽

Huiping Guo

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Disease Patient ◽

Control Group ◽

Severe Coronary Artery Disease ◽

Tumor Treatment ◽

Severe Coronary Artery ◽

The Difference ◽

Artery Disease ◽

New Onset

Background. Arrhythmia is one of the causes of death in severe coronary artery disease patients who also suffered from cancer. Our research aims to compare the incidence of arrhythmia between severe coronary artery disease patient with and without new-onset tumor. Methodology. We enrolled 79 patients (December 2019–December 2020) with severe coronary artery disease in this study, and 40 of them were complicated with new-onset tumor. The details of all subjects were thoroughly obtained; the laboratory tests were implemented including creatinine before coronary angiography. The appraisal of the severity of coronary artery disease was applied by Gensini score. The cardiac inspection includes UCG, 12-lead ECG, and Holter monitor. Results. Among them, there were 40 patients in the experimental group and 39 patients in the control group. The difference at the baseline between the two sets of figures was not statistically significant ( P > 0.05 ). The incidence of arrhythmia between the two groups was statistically significant ( P < 0.05 ). Conclusions. The incidence of arrhythmia in severe coronary artery disease patients who were complicated with new-onset tumor was higher than that in patients with severe coronary artery disease alone, and attention should be paid to arrhythmia before tumor treatment.

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Profile of Estrogen Metabolism in Endometriosis Patients

Indonesian Journal of Obstetrics and Gynecology ◽

10.32771/inajog.v2i3.394 ◽

2016 ◽

Author(s):

Tirsa Verani ◽

Kanadi Sumapradja

Keyword(s):

Blood Level ◽

Obstet Gynecol ◽

Statistical Significance ◽

Cross Sectional Study ◽

Enzyme Linked Immunosorbent Assay ◽

Estrogen Metabolism ◽

Control Group ◽

Cross Sectional ◽

Keywords Endometriosis ◽

Test Result

Objective: To assess the estrone (E1), estradiol (E2) and estriol (E3) blood level and its ratio (E2:E1, E2:E3 and E1:E3) between women with and without endometriosis. Method: We performed an analytical cross sectional study with 27 women with endometriosis and 27 women without endometriosis who met the inclusion criteria. The samples were recruited in Dr. Cipto Mangunkusumo hospital and other satellite hospitals from October 2012 to April 2013. The blood level of estrogen metabolites was examined by enzyme-linked immunosorbent assay (ELISA). Comparison between the two groups was analyzed by using Mann- Whitney test. Result: The level of estrone was found to be lower in endometriosis group compared to that in the control group (54.66 pg/ml vs 73.52 pg/ml, p=0.229). Similarly, the levels of estradiol and estriol were lower in endometriosis group (29 pg/ml vs 35 pg/ml, p=0.815 and 1.11 pg/ml vs 1.67 pg/ml, p=0.095, consecutively). The E2:E1 ratio was higher in endometriosis group (0.51 pg/ml vs 0.38 pg/ml, p=0.164), as well as E2:E3 ratio (26.53 pg/ml vs 21.11 pg/ml, p=0.223) and the E1:E3 ratio (58.55 pg/ml vs 50.28 pg/ml, p=0.684). However, all those differences were not statistically significant. Conclusion: The estrone, estradiol and estriol levels in women with endometriosis were lower compared to those in women without endometriosis. The ratio of E2:E1, E2:E3 and E1:E3 were higher in endometriosis group. However, all those differences failed to reach statistical significance. [Indones J Obstet Gynecol 2014; 3: 127-131] Keywords: endometriosis, estradiol, estriol, estrogen, estrone

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Περιεγχειρητική κινητική αγγειορυθμιστικών παραγόντων σε ασθενείς με καρκίνο του μαστού

10.12681/eadd/36972 ◽

2014 ◽

Author(s):

Γεώργιος Γεωργίου

Keyword(s):

Breast Cancer ◽

Breast Adenocarcinoma ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Study Group ◽

Rt Pcr ◽

Altered Expression ◽

Female Patients ◽

Significant Difference ◽

Circulating Levels

Βackground: angiogenesis is seen during the multiple stages of carcinogenesis, aswell as during the process of surgical wound healing, a fact which has led tosubstantial debate over the last decades about the potential impact of surgery upon thefinal outcome of ceratin patients treated for breast cancer.Aim: the present research aims at investigating the potential effect of surgery on theprocess of angiogenesis, by studying a number of factors that are related to the latter,in patients suffering from breast cancer before and after the time of the procedure,whilst comparing these results with those of patients that were operated on their breastfor non-malignant disease.Material-Methods: blood from 10 female patients with breast adenocarcinoma(Study Group) was collected via venipuncture before surgery (labeled as PRO), aswell as on post-operative day 3 (labeled as D3) and day 7 (labeled as D7). Moreover,blood samples were also taken from 6 female patients with fibroadenoma (ControlGroup) before surgery (PRO) and on day 3 afetr surgery (D3). These samples weremeasured for detection of circulating levels of three established angiogenesisbiomarkers using ELISA (Enzyme-Linked ImmunoSorbent Assay): VascularEndothelial Growth Factor-A (VEFG-A), Interleukin-8 (IL-8) and basic FibroblastGrowth factor (bFGF or FGF-2). In addition, circulating transcripts of 84 agiogenesirelatedgenes were determined using RT-PCR (Real Time Polymerase ChainReaction). The two groups of patients were firstly compared to each other regardingtheir results. Also, patients belonging to the Study Group were analized at differenttime points regarding surgery. Finally, the results were investigated againstclinicopathological data and patient outcome.Results: using ELISA we were able to detect increased levels of circulating VEGF-Aand IL-8 in the Study Group patients compared to the Control Group patientspreoperatively (p=0,0381 and p=0,0218 respectively), while for bFGF there was nostatistically significant difference documented. Surgery resulted in a significantincrease in VEGF-A levels on D3 (p=0,0389) and D7 (p=0,0172) as compared toPRO levels. Perioperative kinetics of IL-8 showed a mild trend towards increase,which, however, was not statistically significant. Postoperative levels of bFGF wereslightly increased on D3, but on D7 they were even lower than preoperative values(p=0,0205). Using RT-PCR certain differences between the Study Group and theControl Group were recorded regarding the circulating transcripts of a great numberof angiogenesis-related genes preoperatively: upregulation of VEGF-C, EGF, IL-8,FGF-1, SPHK1, NRP1, LAMA5, COL4A3, TEK, EFNA3, EFNB2. AKT1, ITGB3,THBS1, CCL11, TIMP3 and downregulation of CXCL10. Moreover, mastectomyinduced an altered expression in several key-genes in breast cancer patients:upregulation of THBS1, COL4A3, BAI1, ITGB3 and downregulation of EREG,SERPIFN1, CXCL9, CXCL10, IL1B, CCL2, CXCL1, HIF1A, NOTCH4. Conclusions: patients suffering from breast cancer have a different angiogenic profilein comparison to patients with fibroadenoma, as documented through their differencesin circulating levels of angiogenic factors. These levels are greatly changed after thesurgical procedure. VEGF showed a transient increase, while bFGF initially increasedbut only to finally decrease to levels that were even lower than the preoperative ones.Moreover, mastectomy promoted a shift in the expression pattern of a broad panel ofangiogenesis-related gene transcripts.

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Analysis of Nursing Cooperation in Microscopic Pituitary Tumor Resection via Transsphenoidal Approach

Tobacco Regulatory Science ◽

10.18001/trs.7.6.119 ◽

2021 ◽

Vol 7 (6) ◽

pp. 6445-6452

Author(s):

Haijuan Hu ◽

Yishu Zhao ◽

Jianhua Ma

Keyword(s):

Statistical Significance ◽

Tumor Resection ◽

Nursing Intervention ◽

Transsphenoidal Approach ◽

Control Group ◽

Mechanical Equipment ◽

Study Group ◽

Observation Group ◽

The Difference ◽

Better Than

To analyze the clinical effect of nursing cooperation in transsphenoidal approach microscopic hypophysectomy. From January 2017 to January 2020, 80 patients who underwent transsphenoidal microscopic hypophysectomy in our hospital were selected to participate in the analysis and study. They were divided into two groups according to the randomized allocation, namely the observation group and the control group. Among them, 40 patients in the observation group and 40 patients in the control group were given routine nursing care for the patients in the control group, and comprehensive nursing intervention was adopted for the patients in the observation group, and the overall nursing effect of the two groups of patients was compared. After taking different nursing methods, the condition of patients in both groups was effectively controlled, and the effective rate of patients in the observation group with comprehensive nursing intervention was significantly better than that of patients in the control group with conventional nursing methods, and the difference had certain statistical significance (P < 0.05); The satisfaction degree of patients in the study group was significantly better than that of patients in the control group, and the difference was statistically significant (P < 0.05). The degree of negative emotions of patients in the study group was significantly better than that of patients in the control group after receiving comprehensive nursing intervention, and the difference was statistically significant (P < 0.05), and the difference in the incidence of adverse events between the two groups was not statistically significant (P > 0.05); The scores of each index of SF-36 questionnaire of patients in both groups were higher than those before nursing, and the scores of each index of patients in observation group were higher than those of patients in control group, and the difference was statistically significant (P > 0.05). With adequate preoperative preparation and mastery of the use of mechanical equipment, comprehensive nursing intervention can effectively improve the treatment effect of patients, make patients more satisfied with the nursing work, and can soothe patients’ negative psychological mood, eliminate panic, improve patients' life confidence, enhance intraoperative cooperation, and ensure that the operation can be completed smoothly.

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Assessment of Serum Concentrations of Adropin, Afamin, and Neudesin in Children with Type 1 Diabetes

BioMed Research International ◽

10.1155/2019/6128410 ◽

2019 ◽

Vol 2019 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Agnieszka Polkowska ◽

Izabela Elżbieta Pasierowska ◽

Marta Pasławska ◽

Elżbieta Pawluczuk ◽

Artur Bossowski

Keyword(s):

Type 1 Diabetes ◽

Glucose Metabolism ◽

Statistical Significance ◽

Serum Levels ◽

Control Group ◽

Diabetic Patients ◽

Serum Concentrations ◽

Novel Biomarkers ◽

Varied Duration

Introduction. The increasing knowledge of adropin, afamin, and neudesin and the regulation of glucose metabolism and insulin resistance allows for the assessment of the differences in their concentrations between the groups with varied duration of diabetes mellitus (DM). Aim of the Study. Assessment of serum levels of adropin, afamin, and neudesin in children with type 1 diabetes, with respect to the disease duration. Materials and Methods. The study consisted of 138 patients aged 5–18 years (M 40.58%). Children with type 1 diabetes (n = 68) were compared to the control group (n = 70). The diabetic group was divided into 4 subgroups: (I) newly diagnosed patients, after an episode of ketoacidosis (n = 14), (II) duration no longer than 5 years (n = 18), (III) 5 to 10 years (n = 27), and (IV) longer than 10 years (n = 9). Serum concentrations of adropin, afamin, and neudesin were assessed and compared between the groups of patients. The criterion for statistical significance was p<0.05. Results. The concentrations of adropin and afamin across all subgroups were lower than that in the control group, while neudesin levels were higher in diabetic patients compared to the control group. The differences were statistically significant. Conclusions. Adropin, afamin, and neudesin may play a major role in the regulation of glucose metabolism and have a significant potential as novel biomarkers to predict future metabolic disorders. However, further multicentre studies on a larger cohort of patients are necessary to specify the role of these substances in the course and treatment of type 1 diabetes.

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