scholarly journals Hematological and Cytometrical Parameters of Japanese Quails Coturnix Coturnix Japonica, Temminck & Schlegel, 1848 as Per Sex and Different Stages of Growth

2021 ◽  
Author(s):  
Ananya Bhattacherjee ◽  
Prafulla Kumar Mohanty ◽  
Bandi Kumar Mallik
Keyword(s):  
Cell Volume ◽  
Japanese Quail ◽  
Packed Cell Volume ◽  
Leukocyte Count ◽  
Age Groups ◽  
Total Leukocyte Count ◽  
Meat Production ◽  
Differential Leukocyte Count ◽  
Coturnix Coturnix ◽  
Length And Area

Abstract This study delineates influence of sex and age on haematological and cytometrical parameters in Japanese quail. Sixty birds were sampled, out of which 20 belong to each age-group (chicks, growers and adults). Haemoglobin, Total Erythrocyte Count, Packed Cell Volume, Erythrocyte indices, Total Leukocyte Count and Differential Leukocyte Count for all 3 age groups and cytometry of blood cells for chicks were analysed. Haemoglobin and packed cell volume of chicks differs significantly with other age-groups at p < 0.01. Significant differences at p = 0.01, p < 0.01 and p < 0.05 observed in lymphocytes, monocytes and Heterophil to Lymphocyte ratio between chicks and growers. Length, length to breadth, nuclear length, breadth, area and Nucleus to Cytoplasm of erythrocytes, length and area of lymphocytes, breadth and area of heterophils also vary significantly at p < 0.01 between Japanese quail chick male and female. Moreover, breadth of erythrocyte and length of heterophil differs significantly at p < 0.05 between two groups. Thus, age and sex influence physiology and hence haematology and may therefore affect egg and meat production.

Incidence and Haematological Changes in Haemoprotozoan Infections in Bovines of South Gujarat

2017 ◽  
Vol 13 (02) ◽  
Author(s):  
M. D. Patel ◽  
Niranjan Kumar ◽  
P. H. Rathod ◽  
K. K. Tyagi ◽  
L. M. Sorathiya
Keyword(s):  
Risk Factors ◽  
Packed Cell Volume ◽  
Leukocyte Count ◽  
Staining Technique ◽  
Total Leukocyte Count ◽  
Research Station ◽  
Normal Range ◽  
Differential Leukocyte Count ◽  
Incidence Risk ◽  
Haematological Changes

A study was conducted to record the incidence, risk factors and haematological changes during haemoprotozoan infections of bovines over a period from January, 2014 to December, 2016. A total of 193 suspected cases (141 cattle and 52 buffaloes) presented at Teaching Veterinary Clinical Complex and Livestock Research Station, Navsari Agricultural University, Navsari, Gujarat, were examined for the presence of haemoprotozoan parasites by Giemsa staining technique. Total 46 (23.83%) cases (23 each of cattle and buffaloes) were found positive for presence of haemoparasite infection. The effect of source of sample, season and species on incidence of haemoprasitic infections was significant whereas effect of year was non-significant. Higher incidence was observed in rainy (34.92) followed by winter (25.42 %) and summer (12.68%) season. Significantly higher incidence of Anaplasmosis (8.29%) was observed followed by Babesiosis (6.74%), Theileriosis (3.63%) and Trypanosomosis and Ehrlichiosis (2.59% each). All positive cases showed comparatively lower values of haemoglobin (Hb.), packed cell volume (PCV) and total erythrocyte count (TEC) whereas values of total leukocyte count (TLC) and differential leukocyte count (DLC) were within the normal range.

scholarly journals Effect of garlic and vitamin B-complex in lead acetate induced toxicities in mice

1970 ◽  
Vol 6 (2) ◽  
pp. 203-210 ◽  
Author(s):  
MSH Khan ◽  
M Mostofa ◽  
MS Jahan ◽  
MA Sayed ◽  
MA Hossain
Keyword(s):  
Cell Volume ◽  
Packed Cell Volume ◽  
Lead Acetate ◽  
Leukocyte Count ◽  
Erythrocyte Count ◽  
Total Leukocyte Count ◽  
Vitamin B ◽  
Group B ◽  
Haemoglobin Content ◽  
Serum Glutamate

The experiment was performed on 25 adult mice, (Swiss albino), weighing about 25 gm, maintained on standard pellet diet and drinking water ad libitum. All the mice were randomly divided into five equal groups (5x5). Each group comprised of five mice was marked as group A, B, C, D and E. In the present study an attempt has been taken to study the effect of Garlic and vitamin B-complex in lead induced toxicities in mice with the observation of some parameters as toxic signs and body weight, some haematological parameters like total erythrocyte count (TEC), total leukocyte count (TLC), haemoglobin content (Hb%) and Packed cell volume (PCV), some biochemical parameters such as serum glutamate pyruvate transaminase SGPT/ALT, Serum glutamate Oxaloacetate transaminase (SGOT/AST) and postmortem changes in mice. An attempt was also made to estimate the residual deposition of lead in body tissues i.e. blood, liver, kidney, brain and bone in mice. Mice treated with lead acetate showed severe toxic signs and significantly reduced total erythrocyte count, total leukocyte count, haemoglobin content and packed cell volume and significant elevation of SGPT and SGOT. But in case of mice treated by lead acetate along with Garlic and vitamin B-complex showed almost normal levels of haematological and biochemical parameters. From this experiment it is revealed that Garlic and vitamin B-complex has protective and curative effect in lead toxicity. The mean body weight of mice of group B (only lead acetate) was significantly (p < 0.01) decreased, in comparison with the other treated groups. Lead acetate group also significantly reduced (p < 0.01) total erythrocyte count, packed cell volume and hemoglobin content was on 42 days of treatment. SGPT and SGOT were significantly elevated in all treated groups in comparison with control group. The liver of the mice of group B were blackish discoloration and enlarged. The significant reduction of lead in blood, liver and kidney was found in group E < C< D in comparison to group B (lead treated). The order of effectiveness was Garlic + Vitamin B- complex > Garlic > Vitamin Bcomplex. Key words: Garlic, vitamin B-complex, lead acetate, mice   doi: 10.3329/bjvm.v6i2.2337 Bangl. J. Vet. Med. (2008). 6 (2): 203-210

scholarly journals Immune function during pregnancy varies between ecologically distinct populations

2020 ◽  
Vol 2020 (1) ◽  
pp. 114-128
Author(s):  
Carmen Hové ◽  
Benjamin C Trumble ◽  
Amy S Anderson ◽  
Jonathan Stieglitz ◽  
Hillard Kaplan ◽  
...  
Keyword(s):  
Leukocyte Count ◽  
Total Leukocyte Count ◽  
Future Research ◽  
Differential Leukocyte Count ◽  
Ecological Conditions ◽  
Trade Offs ◽  
The Usa ◽  
Immunological Changes ◽  
Fetal Antigens ◽  
Pathogen Exposure

Abstract Background and objectives Among placental mammals, females undergo immunological shifts during pregnancy to accommodate the fetus (i.e. fetal tolerance). Fetal tolerance has primarily been characterized within post-industrial populations experiencing evolutionarily novel conditions (e.g. reduced pathogen exposure), which may shape maternal response to fetal antigens. This study investigates how ecological conditions affect maternal immune status during pregnancy by comparing the direction and magnitude of immunological changes associated with each trimester among the Tsimane (a subsistence population subjected to high pathogen load) and women in the USA. Methodology Data from the Tsimane Health and Life History Project (N = 935) and the National Health and Nutrition Examination Survey (N = 1395) were used to estimate population-specific effects of trimester on differential leukocyte count and C-reactive protein (CRP), a marker of systemic inflammation. Results In both populations, pregnancy was associated with increased neutrophil prevalence, reduced lymphocyte and eosinophil count and elevated CRP. Compared to their US counterparts, pregnant Tsimane women exhibited elevated lymphocyte and eosinophil counts, fewer neutrophils and monocytes and lower CRP. Total leukocyte count remained high and unchanged among pregnant Tsimane women while pregnant US women exhibited substantially elevated counts, resulting in overlapping leukocyte prevalence among all third-trimester individuals. Conclusions and implications Our findings indicate that ecological conditions shape non-pregnant immune baselines and the magnitude of immunological shifts during pregnancy via developmental constraints and current trade-offs. Future research should investigate how such flexibility impacts maternal health and disease susceptibility, particularly the degree to which chronic pathogen exposure might dampen inflammatory response to fetal antigens. Lay Summary This study compares immunological changes associated with pregnancy between the Tsimane (an Amazonian subsistence population) and individuals in the USA. Results suggest that while pregnancy enhances non-specific defenses and dampens both antigen-specific immunity and parasite/allergy response, ecological conditions strongly influence immune baselines and the magnitude of shifts during gestation.

scholarly journals The mongolian gerbil: qualitative and quantitative aspects of the cellular blood picture

1975 ◽  
Vol 9 (4) ◽  
pp. 283-287 ◽  
Author(s):  
Walter G. Dillon ◽  
Chester A. Glomski
Keyword(s):  
Haemoglobin Level ◽  
Cell Volume ◽  
Mongolian Gerbil ◽  
Leukocyte Count ◽  
Absolute Number ◽  
Total Leukocyte Count ◽  
Red Cell ◽  
Blood Picture ◽  
Qualitative And Quantitative ◽  
Circulating Lymphocytes

The gerbil displays sex determined differences in packed red-cell volume, haemoglobin level, total leukocyte count and absolute number of circulating lymphocytes. The erythrocytes demonstrate a prominent polychromasia and frequent occurrence of basophilic stippling.

EFFECT OF AGE AND PLANE OF NUTRITION ON THE BLOOD CHEMISTRY OF THE COLUMBIAN BLACK-TAILED DEER (ODOCOILEUS HEMIONUS COLUMBIANUS): A. PACKED-CELL VOLUME, SEDIMENTATION RATE, AND HEMOGLOBIN

1956 ◽  
Vol 34 (5) ◽  
pp. 477-484 ◽  
Author(s):  
W. D. Kitts ◽  
P. J. Bandy ◽  
A. J. Wood ◽  
I. McT. Cowan
Keyword(s):  
Cell Volume ◽  
Sedimentation Rate ◽  
Packed Cell Volume ◽  
Age Groups ◽  
Odocoileus Hemionus ◽  
Blood Chemistry ◽  
Odocoileus Hemionus Columbianus ◽  
Blood Constituents ◽  
Significant Difference ◽  
Effect Of Age

A study has been made of the normal chemistry of the blood constituents of the Columbian black-tailed deer (Odocoileus hemionus columbianus) in relation to growth and the caloric plane of nutrition. The results showed a significant difference of packed-cell volume and hemoglobin level between the two age groups of experimental animals, while no significant differences were found in the sedimentation rates. The high and low imposed planes of nutrition did not bring about a significant difference in packed-cell volume, sedimentation rate, or hemoglobin value.

scholarly journals Bacterial infection in wheezy children.

2021 ◽  
Vol 28 (02) ◽  
pp. 176-180
Author(s):  
Maria Saleem ◽  
Asim Khurshid ◽  
Waqas Imran Khan ◽  
Amna Wajdan
Keyword(s):  
Bacterial Infection ◽  
Absolute Neutrophil Count ◽  
Neutrophil Count ◽  
Leukocyte Count ◽  
Tertiary Care ◽  
Case Series ◽  
Total Leukocyte Count ◽  
X Rays ◽  
Differential Leukocyte Count ◽  
Case Series Study

Objective: To determine the frequency of bacterial infection in children less than five years of age presenting with respiratory wheeze to Tertiary care centre. Study Design: Descriptive Case Series study. Setting: Department of Pediatrics, The Children’s Hospital and The Institute of Child Health, Multan. Period: November 2019 to April 2020. Material & Methods: A total of 130 children, presenting with respiratory symptoms were examined for presence of respiratory wheeze. After confirmation of chest auscultation, patients were enrolled. Information regarding duration of wheeze, axillary temperature, presence of chest in drawing and respiratory rate were recorded. Appropriate blood sample were drawn for total leukocyte count and differential leukocyte count from which absolute neutrophils count was calculated. Children were evaluated for the presence of bacterial infection as per operational definition. Results: There were 38 (29.23%) female and 92 (70.76%) male patients. Overall, mean age was13.17±5.49 with age range of 1-57 months. Bacterial infection was noted in 33 (25.38%) patients. Out of 130 patients presenting with wheeze, 50 patients (38.46%) had temperature range of 98.1-990F. Absolute neutrophil count was more than 5000/dl in 42(32.30%) patients, out of these 33(78.6%) had bacterial infection. Chest X-rays were showing infiltrates in 110 (84.6%) patients. Conclusion: Children suffering from wheeze have bacterial infection if age is less than five years and there is fever, crepitations, elevated absolute neutrophil count and radiographic evidence of pneumonia.

Role of interferon gamma release assay in the diagnosis of Pott disease

2010 ◽  
Vol 12 (5) ◽  
pp. 462-466 ◽  
Author(s):  
Raj Kumar ◽  
Rupant K. Das ◽  
Ashok Kumar Mahapatra
Keyword(s):  
Mr Imaging ◽  
Bone Scan ◽  
Leukocyte Count ◽  
Total Leukocyte Count ◽  
Enzyme Linked Immunosorbent Assay ◽  
Interferon Gamma Release Assay ◽  
Vertebral Collapse ◽  
Diagnostic Dilemma ◽  
Differential Leukocyte Count ◽  
Radiological Criteria

Object Tuberculosis is ubiquitous in its presence and in its myriad manifestations. Despite several advances, it often poses a diagnostic dilemma. The aim of this study was to evaluate the efficacy of the QuantiFERON assay in the diagnosis of Pott disease. Methods In this study, 70 consecutive patients with vertebral collapse underwent a battery of investigations including radiological evaluation (MR imaging and chest and spine radiography), routine blood workup (hemoglobin, total leukocyte count, differential leukocyte count, and erythrocyte sedimentation rate), enzyme-linked immunosorbent assay (ELISA) for tuberculosis, a bone scan and the QuantiFERON assay, perioperative evaluation, and bacteriological studies. The patients were then classified as having tuberculosis on the basis of positive smear or culture, a biopsy consistent with tuberculosis, or a therapeutic response to antituberculosis chemotherapy on follow-up. The efficacy of the investigations, both individually and in combination, were evaluated. Results Tuberculosis was diagnosed in 51 patients, and 19 had vertebral collapse that was attributable to other causes. Based on the authors' data, sensitivity of the QuantiFERON assay in the diagnosis of tuberculosis of the spine was 84% and specificity was 95%. In combination with radiological criteria, bone scan, and ELISA, the QuantiFERON assay was predictive of tuberculosis in 90% of cases (46 patients). Conclusions None of the investigative modalities individually are efficacious enough to make a diagnosis of tuberculosis of the spine with such a degree of certainty as to to obviate the need for a tissue diagnosis or bacteriological confirmation. The ELISA, MR imaging of the spine, and bone scanning findings provide a reasonably certain diagnosis in 90% of cases, providing the grounds for starting a safe trial of antituberculous chemotherapy.

scholarly journals Further observations on the packed cell volume and haemoglobin concentration in cattle naturally infected with Fasciola gigantica

1970 ◽  
Vol 2 (2) ◽  
pp. 125-127
Author(s):  
MMR Howlader ◽  
S Begum ◽  
KhN Islam ◽  
MA Hai ◽  
MG Hossain
Keyword(s):  
Cell Volume ◽  
Packed Cell Volume ◽  
Age Groups ◽  
Haemoglobin Concentration ◽  
Fasciola Gigantica ◽  
Zebu Cattle ◽  
Infected Cattle ◽  
Infected Control ◽  
Hb Concentration ◽  
Effect Of Age

The changes on packed cell volume (PCV) and haemoglobin (Hb) in zebu cattle infected with Fasciola gigantica were studied in two age and two season groups during the period from November 1999 to June 2000. Eighty-eight selected cattle were divided into two age groups, 2.5 to 5.5 years (n = 44; 22 infected & 22 uninfected control) and 6.0 to 9.0 years (n = 44; 22 + 22 cattle), and similarly two seasons, winter (n = 22 + 22) and summer (n = 22 + 22) were used for this study. Blood samples were collected in glass vials contained EDTA anticoagulant for each age group of all animals in summer and winter seasons. PCV was determined using microhematocrit and Hb concentration by cyanomethemoglobin methods. The PCV of F. gigantica infected cattle were significantly (p < 0.01) lower than the non-infected control animals. The average PCV obtained were 24.43% and 32.33% for F. gigantica infected and non-infected cattle, respectively. The Hb concentrations of infected cattle were significantly (p < 0.01) lower than the non-infected control animals. The average Hb values found were 7.59 and 10.13 g% for F. gigantica infected and noninfected cattle, respectively. There was no significant effect of age and season on the values of PCV and Hb concentrations in infected and non-infected cattle.Key words: Zebu cattle; Fasciola gigantica; packed cell volume; haemoglobin concentrationdoi: 10.3329/bjvm.v2i2.2553Bangl. J. Vet. Med. (2004). 2 (2): 125-127

scholarly journals Evaluation of Effect of Melatonin on Hematological Parameters in Patients of Periodontitis

2019 ◽  
pp. 46-49
Author(s):  
Anagha Marawar ◽  
Pramod Marawar ◽  
Nandal D H ◽  
Rahul Kunkulol ◽  
Sandeep Narwane
Keyword(s):  
Leukocyte Count ◽  
Hematological Parameters ◽  
Total Leukocyte Count ◽  
Differential Leukocyte Count ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Root Planning ◽  
Scaling And Root Planning

Introduction: Considering the immunity enhancing property of melatonin, a study on the evaluation of the effect of melatonin on the hematological parameters in patients suffering from Periodontitis.  Methods: This study was conducted in the Department of Periodontics, Rural Dental College, Loni. Patients of chronic Periodontitis, of age between 18 to 65 years of either gender ready to give informed consent to participate in the study were included. Postoperative patients, patients having night duties, drivers and those using heavy machinery, pregnant women, lactating mothers, patients with any clinically significant systemic disease and patients on any other drugs were excluded from the study. Patients were divided into two Groups. Group B received scaling and root planning (SRP) and melatonin 3 mg per day were compared to Group A which received SRP only. The Total Leukocyte count, Differential Leukocyte count and Erythrocyte sedimentation rate of each patient was studied at a baseline, 1st (30 days) visit, 2nd (60 days) visit and 3rd (90 days) visit. Results: Both the groups consisted of 80 subjects each. Group A consisted of 71.25% male and 28.75% female patients. There were 56.25% male and 43.75% female patients in Group B. With respect to visit 3, the TLC was statistically lower in Group B. The neutrophil count of Group B was significantly lower during visit 3 as compared to Group A. The lymphocyte count of Group B was significantly lower than that of Group A during the visit 1, and also significantly higher during visit 3 on comparison with Group A. There was no statistically significant difference in the groups with respect to eosinophil and basophil count. The Monocyte count of Group B was statistically lower when compared to that of Group A. There was no statistically significant difference between ESR of Group A & Group B.  Conclusions: It can be concluded that melatonin has a positive effect on TLC and differential count of patients of periodontitis, conferring a new facet to the management of periodontitis and an attempt to impede the disease progression. Keywords: Melatonin; Hematological parameters; Periodontitis; Total Leukocyte count; Differential Leukocyte count; Erythrocyte sedimentation rate; Scaling and Root Planning.

scholarly journals Screening for Toxic and Haematological Effect of Ethanol Extract of Azadirachta Indica in Wistar Rats Using Some Serum Enzymes as Markers

2020 ◽  
Vol 13 (1) ◽  
pp. 113-119
Author(s):  
K.K. Igwe ◽  
I.E. Otuokere ◽  
O. V. Ikpeazu
Keyword(s):  
Body Weight ◽  
Azadirachta Indica ◽  
Leukocyte Count ◽  
Negative Control ◽  
Serum Enzymes ◽  
Haematological Parameters ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Differential Leukocyte Count ◽  
Treatment Groups

The leaves of Azadirachta indica plant was screened for haematological, toxic and serum enzyme activities in rats. Twenty rats were used and were grouped into 4 of 5 rats each. Group 1 was the negative control group administered distilled water. Groups 2, 3, and 4 were the treatment groups received 200, 400 and 800 mg/kg body weight of the A. indica extract respectively. The rats were dosed for 14 days, thereafter were sacrificed and blood collected by cardiac puncture for analysis. The effect of A. indica extract was checked on haematological parameters and serum enzymes activities. All results in treatment groups were compared with the normal control at statistical confidence of 95% (p<0.05). There was progressive reduction of haematological parameters as the dose of the extract increased from 200, 400, to 800 mg/kg body weight. Haematological parameters, PCV, RBC, Haemoglobin showed decrease value which was not statistically significant at (p<0.05). Total leukocyte count, showed progressively elevation by the extract though not statistically significant. Differential leukocyte count indicated very mild lymphocytosis neutropeania, monocytopeania and eosinopeania which were not statistically significant. Clinical biochemical parameters, A. indica extract demonstrated normal levels of the serum enzymes (AST, ALT and ALP) though there was slight decrease in a dose dependent fashion.Total protein was within normal range. The normal MCV, MCH and MCHC values suggests normocytic normochromic anaemic condition. The extract of A. indica is safe to blood cells, liver and kidney marker enzymes at dose < 800 mg/kg body weight.

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