scholarly journals Development of a Novel Differential Duplex PCR for Pathogenic and Intermediate Leptospires: First Report of Intermediate Leptospira in Animal Sera in Argentina

Author(s):  
Olivia Watanabe ◽  
Flavia C. Hasenauer ◽  
Bibiana F. Brihuega ◽  
Vanina R. Saraullo ◽  
Micaela Hamer ◽  
...  

Abstract Background: Even though leptospirosis is one of the most important zoonoses in the world, many of its aspects remain unknown, such as the distribution of different species and their pathogenicity for animals and humans. Results: Intermediate leptospiral DNA was detected in bovine sera samples from animals with abortion history from Argentina. We designed a novel duplex PCR, which differentiates pathogenic and intermediate leptospires in one single step. This new diagnostic method allowed the detection of intermediate leptospiral DNA in 4.38 % (n = 5/114) of the total analyzed bovine samples. Conclusions: These results highlight the need of further studies of intermediate species in our country, especially regarding their association to clinical disease in animals. Remarkably, this is the first report of detection of intermediate leptospires circulating in livestock animals in Argentina and the second report in the entire world.

2020 ◽  
Vol 14 (3) ◽  
pp. 112-122
Author(s):  
A. N. Chumakov ◽  
P. S. Yurchenko

The coronavirus pandemic has generated a lot of talk about the post-coronavirus future of the world community and has drawn close attention to the globalization process. The article notes that something similar in the public consciousness took place after the publication of the first report to the Club of Rome, when the awareness of the danger posed by global problems had quickly come. Now, one of the global problems — the coronavirus pandemic — has changed the usual life rhythm of the entire world community in a matter of weeks. The relevance of the topic is so high that even those who, before that, not only professionally, but even seriously were not interested in either the problems of medicine or global problems, actively joined its discussion. As a result, on the pages of many print and electronic publications, along with serious and verified information, all sorts of guesses and speculations about the nature and correlation of coronavirus and globalization are multiplied. Only knowledge can be opposed to this, according to the authors.


ENTOMON ◽  
2020 ◽  
Vol 44 (4) ◽  
pp. 311-314
Author(s):  
A. Roobakkumar ◽  
H.G. Seetharama ◽  
P. Krishna Reddy ◽  
M.S. Uma ◽  
A. P. Ranjith

Rinamba opacicollis Cameron (Hymenoptera: Braconidae) was collected from Chikkamagaluru, Karnataka, India for the first time from the larvae of white stem borer, Xylotrechus quadripes Chevrolat infesting arabica coffee. Its role in the biological or integrated control of X. quadripes remains to be evaluated. White stem borer could be the first host record of this parasitoid all over the world.


2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Si-Yang Huang ◽  
Jing-Zhi Gong ◽  
Yi-Jun Ren ◽  
Ming Pan ◽  
Wei-Min Cai ◽  
...  

Abstract Background Fasciola hepatica is an important zoonotic parasite that causes fasciolosis in a broad range of animals. No information is available about the prevalence of F. hepatica in Père David’s deer (Elaphurus davidianus), an endangered species in the world. Therefore, the purpose of the study was to evaluate the prevalence of fasciolosis in Père David’s deer in the Dafeng Elk National Natural Reserve, Jiangsu province, China. Results In this study, 142 fecal samples from Père David’s deer were analyzed for F. hepatica by microscopy and nest-PCR. Only one sample was positive for F. hepatica according to microscopy examination, while 18 of 142 (12.68, 95%CI: 2.841–22.45%) samples were positive for F. hepatica according to nest-PCR results. Conclusions This is the first report of prevalence of F. hepatica in Père David’s deer. The prevalence data indicated that F. hepatica was also present in this endangered animal, which may cause a potential threat to this precious species.


Author(s):  
Hao Jin ◽  
Ligong Lu ◽  
Junwei Liu ◽  
Min Cui

Abstract Motivation Nations around the world have been significantly impacted during the COVID-19 pandemic. China’s strategies for controlling COVID-19 offer valuable lessons for the global community. By learning from China’s experience and lessons, other countries could also find appropriate methods to control the pandemic. Problem statement What measures has China taken to control the pandemic? What lessons has China learned through this pandemic? Approach/methods The literature on China’s lessons and experience in controlling the COVID-19 pandemic was searched and reviewed. Related newspapers and magazines were also searched. Results China’s experience can be summed up as establishing temporary hospitals, strict isolation, experts with a knowledge of COVID-19, and measures that increase social distancing. Conclusions By learning from the experience of China, other countries in the world could eventually find the methods to control the COVID-19 pandemic. An emergency response system should be established in each country. Doctors and nurses are not alone in fighting COVID-19, and the entire world is helping them. With cooperation, current difficulties could be overcome.


Plant Disease ◽  
2021 ◽  
Author(s):  
Luciany Favoreto ◽  
Rafaela Bueno ◽  
Angélica Calandrelli ◽  
Patrícia Priscila França ◽  
Mauricio Conrado Meyer ◽  
...  

Several species of nematodes are known to cause losses to cowpea (Vigna unguiculata) throughout the world. In Brazil, Aphelenchoides besseyi was recently described causing damages on soybean, cotton, and common bean, but no report was found about the parasitism of this nematode in cowpea. The present study aimed to verify the host reaction of cowpea cultivars to A. besseyi. The experiment was conducted under greenhouse conditions, using as inoculum two A. besseyi populations, obtained from symptomatic soybean and cotton plants collected in naturally infested fields. Cultivars ‘Imponente’, ‘Aracê’, ‘Guariba’, ‘Tumucumaque’, ‘Nova Era’, and ‘Tracuateua’ were inoculated with 500 A. besseyi of each population, separately, into soil and after 30 days from the inoculation nematodes were extracted from shoot systems. Both populations were able to parasitize all the cowpea cultivars. Independently of the cultivar, cowpea plants exhibited symptoms of leaf deformation similar to those described for soybean, cotton, and common bean and, in addition, severe brooming was observed and the interior of the stems was porous and necrotic. To our knowledge, this is the first report of parasitism by A. besseyi of cowpea in Brazil, under greenhouse conditions, increasing the list of hosts of this nematode.


2021 ◽  
Vol 9 (10) ◽  
pp. 2423-2427
Author(s):  
Sangeeta Gupta ◽  
Anupama Patra ◽  
Sarita Yadav ◽  
Akanksha Thakur

The entire world faced the corona crisis recently, still undergoing it. The world merely is seeing through it as a pandemic and is connecting it to a kind of viral infection invading the human community. The whole of the health machinery got paralyzed fighting the pandemic leading to millions of deaths around the globe. Moreover, the ad- vanced modern system of medicine was almost helpless in combating the virus-related hazards to human health. At this time, the considerable contribution was provided by the Ayurveda, our ancient traditional system of medi- cine. If we see the ayurvedic literature, the concept of Janpadodhwamsa provides answers to the mystery behind the fatal covid virus. The paper aims to provide a view about the Janpadodhwamsa which states various factors relating to the pandemic, the root cause of such events and the remedial measures for it. Keywords: Vayu, Jala, Desh, Kala, Janpadodhwamsa, Nidana Parivarjana, Prajnapradha


2014 ◽  
Vol 57 (4) ◽  
pp. 13-35
Author(s):  
DOMINIQUE LE TOURNEAU

The faithful have the native right o proclaiming the faith, right which concerns first of all the whole Church, as it was reminded by the II Vatican Council. As far as the faithful are concerned, this duty-right is connected with can. 210 about sanctity of life and can. 225 §1 on working to expand the divine message of salvation throughout the entire world. The Author deals also with the notion of proselytism, which is not always correctly understand.Part II of the present work present “the actors of apostolate”. To exercise their function in the Church and in the world, their right to associate must be recognized and protected so that they be able to do individual apostolate, which does not prevent them from participating in the apostolate organised by the hierarchy of the church. Canon 211 appears to be an essential norm in the Code, and is a direct consequence of secularity of lay people. Anyway, the faithful are subject to a certain control by the diocesan bishop as moderator of the entire ministry of the Word (can. 756 § 2).Concluding, the Author deplore that a true protection of the rights of the faithful is still largely missingin the church.


2018 ◽  
Vol 18 (17) ◽  
pp. 151-158
Author(s):  
Hikmat Bahadur Raya

HIV/AIDS is one of the worst pandemics, the world has ever known and the entire world has been combating with it. Objective of the study is to analyze the global and national trend of HIV/AIDS regarding the indices PLHIV, New infections and AIDS-related death and coverage of ART. This is a quantitative research completely based on the secondary data. The data is analyzed by simple statistical representations. Regression analysis is used to analyze the trends and to make some predictions. The number of PLHIV is increased by 19.9 percent from 1997 to 2015 and by 69.6 percent from 2000 to 2015 in Nepal. In this same duration, the new HIV infection is reduced by 63.8 percent and 82.6 percent, AIDS related death by 52.2 percent and 411.1 percent respectively in the global and Nepal. The initial coverage of ART in both regions was negligible and by the end of 2015 it reached to 46.4 percent and 30.6 percent in the global and Nepal respectively. Nepal along with the global achieves some positive results in the combating of this health problem. By analyzing the trends, it is not worth to say that we are not far from the achievement of SDGs.  


2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Zulaikha Zulaikha

The world in one hand, now is no longer just an advertising tagline. It really has become a reality, when the gadget in our hands is able to present all the information needs of the entire world. Not just information, various entertainment can also be found in gadgets. From movies, music, drama series, talk shows, comedy, and various shows owned by influencers or YouTubers that are entertainment. And special, the audience in gadgets only watch the shows they like. There's no way they would waste their quota to watch a program they don't like. The question is, did television audiences still have an audience in such an era? As we all know, television viewers cannot just watch what they like, because in the program they are watching, it is likely that other programs that they dislike (such as advertisements) are tucked away. This paper tries to reveal, is it true that online media can replace conventional media? Or is it true that conventional media has been abandoned by the audience? The results of this study revealed that not all viewers left conventional media. Similarly, not all programs can be enjoyed online. Keywords: Conventional Media; Online Media; The audience. 


2021 ◽  
Vol 8 (12) ◽  
pp. 310
Author(s):  
Ísis Assis Braga ◽  
Isis Indaiara Gonçalves Granjeiro Taques ◽  
Estefânia Crivelatti Grontoski ◽  
Ingrid Savino de Oliveira Dias ◽  
Nathalia Assis Pereira ◽  
...  

Cats naturally exposed to Ehrlichia canis have been described in different regions of the world, but little is known about the genotypes associated with infection in these animals. To detect E. canis-specific antibodies and investigate the E. canis TRP genotypes in cats, serum samples from 76 domestic cats reactive to crude E. canis antigens by the indirect fluorescence antibody test (IFAT) were analyzed by ELISA, using E. canis-specific peptides (i.e., TRP19 and TRP36 /BR/US/CR). Of these, 25 (32.9%) cats reacted to at least one TRP peptide, confirming their specific exposure to E. canis. Eighteen (23.7%) cats reacted to TRP19, 15 (19.8%) to BRTRP36, and 11 (14.5%) to USTRP36, but none of them reacted to CRTRP36. Eight (10.5%) cats reacted to TRP19 but not to any TRP36 genotype, demonstrating the possible existence of a new E. canis genotype infecting felines. Nevertheless, this study provides the first report of anti-E. canis-specific antibodies in domestic cats.


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