Evaluating The Relationship Between The Primary Damages of Deoxyribonucleic Acid and Total Antioxidant Capacity With The Respiratory Exposure To The Benzene in One of The Oil Companies in Iran

2021 ◽  
Author(s):  
Mohammad Javad Zare Sakhvidi ◽  
Abdorreza Zarei ◽  
Vida Rezaei Hachesu ◽  
Ahmad Zolfaghari
Keyword(s):  
Free Radicals ◽  
Comet Assay ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Deoxyribonucleic Acid ◽  
Petroleum Products ◽  
Control Group ◽  
Exposure Group ◽  
Total Antioxidant ◽  
History Of

Abstract Benzene is a carcinogenic chemical substance which causes the injuries and damages through producing the free radicals in DNA (deoxyribonucleic acid) and the antioxidants are the agents which reduce the impacts of DNA damages by inhibiting the free radicals. This study was conducted aiming at determination of primary damages of DNA and level of plasma oxidative stress markers resulting from the respiratory exposure to the benzene found in petroleum compounds among the workers at loading platforms of a petroleum products distribution center. This study was an analytical (case control) research conducted among the workers in a working shift serving at the loading platforms of petroleum products. The exposure group included the workers with a history of contact with benzene and the control group was composed of the persons with no history of exposure to benzene. To investigate the level of the personnel’s exposure to benzene, NIOSH-1501 method was utilized; to analyze the samples taken from the air, GC mass (Gas chromatography–mass spectrometry) was applied; and to determine the average of DNA primary damages, Comet Assay was used. To investigate %TAC (Total Antioxidant Capacity), the blood serum of the exposure and control groups was applied by using the photometry device in wavelength of 520 nm. Results indicate The average difference of parameters of Tail Length (TL), Tail Density (TD), Tail Momentum (TM), percentage of tail in the DNA (%DNA) and %TAC in control group was 78.59, 8.35, 1.20, 10.05 and 25.58 and in the exposure group was 59.21, 75.74, 57.74, 3.5 and 16.58, respectively. Determining the DNA damage using Comet Assay and examining %TAC by photometry showed that TL, %DNA and %TAC values decreased among the workers already exposed to benzene and TD and TM values increased. In comparing the averages between two studied groups, all study variables had statistically meaningful difference (P < 0.05). More studies are recommended to be conducted on using the methods which identify the special places of breakage and damage in DNA chain due to the exposure to benzene and consequently prevent the its complications and consequences.

Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Male Smokers

2019 ◽  
Vol 22 (7) ◽  
pp. 496-501
Author(s):  
Fatemeh Ahmadi-Motamayel ◽  
Parisa Falsafi ◽  
Hamidreza Abolsamadi ◽  
Mohammad T. Goodarzi ◽  
Jalal Poorolajal
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Free Radicals ◽  
Antioxidant Capacity ◽  
Cigarette Smoke ◽  
Total Antioxidant Capacity ◽  
The Body ◽  
Total Antioxidant ◽  
The Mean ◽  
And Oxidative Stress

Background: Cigarette smoke free radicals can cause cellular damage and different diseases. All the body fluids have antioxidants which protect against free radicals. Objective: The aim of this study was to evaluate salivary total antioxidant capacity and peroxidase, uric acid and malondialdehyde levels in smokers and a nonsmoking control group. Methods: Unstimulated saliva was collected from 510 males. A total of 259 subjects were current smokers and 251 were non-smokers. The levels of salivary total antioxidant capacity, uric acid, peroxidase and malondialdehyde were measured using standard procedures. Data were analyzed with t test and ANOVA. Results: The smokers were younger and dental hygiene index was higher than healthy nonsmoking controls. The mean total antioxidant capacity in smokers and nonsmokers was 0.13±0.07 and 0.21±011, respectively (P=0.001). Smokers had significantly lower peroxidase and uric acid levels than healthy controls. In addition, the mean malondialdehyde levels in the smokers and nonsmokers were 4.55 ±2.61 and 2.79 ±2.21, respectively (P=0.001). Conclusion: Cigarette smoke produces free radical and oxidative stress, causing many side effects. Salivary antioxidant levels decreased and malondialdehyde levels increased in smokers, indicating the high oxidative stress among smokers compared to nonsmokers. Cigarette smoke had deleterious effects on main salivary antioxidants levels.

scholarly journals Protective Effect of Matricaria chamomilla Ethanolic Extract on Hippocampal Neuron Damage in Rats Exposed to Formaldehyde

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Zahra Sayyar ◽  
Alireza Yazdinezhad ◽  
Maryam Hassan ◽  
Iraj Jafari Anarkooli
Keyword(s):  
Cell Death ◽  
Free Radicals ◽  
Antioxidant Capacity ◽  
Passive Avoidance ◽  
Avoidance Learning ◽  
Total Antioxidant Capacity ◽  
Ethanolic Extract ◽  
Passive Avoidance Learning ◽  
Matricaria Chamomilla ◽  
Total Antioxidant

Formaldehyde, as a frequently used compound in many applications, crosses the blood-brain barrier and leads to hippocampal cell death and memory impairment. This study investigates the effects of ethanolic extract of Matricaria chamomilla (MC) on passive avoidance learning induced by damaged hippocampal cells and evaluates the antioxidant traits of MC. The male Wistar rats were divided into six (6 in each) groups: control (10 mg/kg normal saline), 200 (200 mg/kg MC extract), 500 (500 mg/kg MC extract), F (10 mg/kg formaldehyde), F200 (10 mg/kg formaldehyde and 200 mg/kg MC extract), and F500 (10 mg/kg formaldehyde and 500 mg/kg MC extract). Shuttle box assay was used for evaluation of passive avoidance learning. The apoptosis rate of hippocampal tissue, malondialdehyde (MDA) free radicals, and total antioxidant capacity was evaluated to determine the positive effect of the ethanolic extract of MC. We found that the ethanolic extract of MC reduced the cell death, time spent in a dark room, and MDA free radicals in the hippocampus, leading to increased total antioxidant capacity in this region. In conclusion, the ethanolic extract of MC could ameliorate formaldehyde-induced memory damage through decreasing cell death and MDA activity of the hippocampal region and increasing total antioxidant capacity.

scholarly journals Radioprotective and anti-diabetic effects of Costus speciosus and carnosine

2020 ◽  
Vol 19 (1) ◽  
pp. 121-127
Author(s):  
Ebtisam A. Marzook ◽  
Fawzy A. Marzook ◽  
Ahmed E. Abd El Moneim
Keyword(s):  
Gamma Radiation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Natural Antioxidants ◽  
Serum Levels ◽  
Serum Glucose ◽  
Control Group ◽  
Albino Rats ◽  
Costus Speciosus ◽  
Total Antioxidant

Purpose: To evaluate the possible radioprotective effect of Costus speciosus and carnosine as natural antioxidants in order to control the  hyperglycemia developed in male albino rats exposed to acute oxidative stress induced by gamma radiation. Methods: Twenty-eight adult male albino rats were divided into four groups. The first group was taken as a control group, while the three other groups were exposed to Ɣ irradiation at a single 7.5 Gy dose. Furthermore, the rats in the second and third groups were i.p. injected with Costus speciosus root powder and carnosine, respectively. On the 3rd day, after irradiation, the serum levels of glucose, insulin, C peptide, copper, iron, calcium, total antioxidant capacity (TAC) and malondialdehyde (MDA) were measureded. Results: The results revealed that exposure to Ɣ irradiation induced significant increases in serum glucose, iron, and malondialdehyde. However, the levels of serum calcium, copper, total antioxidant capacity and insulin significantly decreased (p < 0.05). A significant decrease was observed in Cpeptide in the exposed group, compared to control group. All the test parameters indicate improvement after treatment with Costus speciosus and carnosine (p < 0.05). Conclusion: Costus speciosus and carnosine ameliorate the effect of gamma radiation, indicating their role as antidiabetic agents and  radioprotectors; however, Costus speciosus was critically more efficient than carnosine. Keywords: Costus speciosus, Carnosine, Diabetes, Insulin, Gamma radiation protection

scholarly journals Evaluation of Salivary and Serum Total Antioxidant Capacity and Lipid Peroxidation in Postmenopausal Women

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Fatemeh Zovari ◽  
Hadi Parsian ◽  
Ali Bijani ◽  
Ameneh Moslemnezhad ◽  
Atena Shirzad
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Postmenopausal Women ◽  
Total Antioxidant Capacity ◽  
Premenopausal Women ◽  
Control Group ◽  
Antioxidant Power ◽  
Whole Saliva ◽  
Total Antioxidant

Objective. In menopause, reduction of estrogen hormone affects oxidative stress process in serum. Oxidative stress in saliva plays a significant role in the pathogenesis of oral diseases. The aim of this study was to investigate the total antioxidant capacity and lipid peroxidation in the serum and saliva of premenopausal and postmenopausal women. Methods. In this case control study, 50 postmenopausal women (case group) and 48 premenopausal women (control group) were selected. The unstimulated whole saliva and serum of the postmenopausal and premenopausal women were collected. The total antioxidant capacity (TAC) of the saliva and serum was measured by ferric reducing antioxidant power (FRAP). Also, malondialdehyde (MDA) was measured by thiobarbituric acid reactive substance (TBARS) method for serum and saliva. Then, the obtained data were analyzed by SPSS 17, whereby Mann–Whitney test and Spearman’s correlation test were used. P < 0.05 was considered statistically significant. Results. The postmenopausal group had significantly lower mean serum TAC and higher mean serum MDA than the control group ( P < 0 < 001 and P < 0.01 , respectively). The mean salivary TAC and MDA, however, did not differ significantly between the case and control group ( P = 0.64 and P = 0.08 , respectively). Conclusion. In postmenopausal women, with elevation of serum MDA and reduction of serum TAC, the extent of serum oxidative stress grows, but MDA and TAC levels of saliva do not change.

scholarly journals Blood Levels of Oxidant/Antioxidant Parameters in Rats Infected withToxoplasma gondii

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Somayeh Bahrami ◽  
Ali Shahriari ◽  
Mehdi Tavalla ◽  
Somayeh Azadmanesh ◽  
Hossein Hamidinejat
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Parasitic Infection ◽  
Chronic Phase ◽  
Antioxidant Defense System ◽  
Female Rats ◽  
Control Group ◽  
Blood Levels ◽  
Total Antioxidant

Toxoplasmosis is a common parasitic infection in the world. Since increased free radicals and oxidative stress are reported in many parasitic diseases the purpose of the present study was to evaluate the oxidative stress in acute and chronic toxoplasmosis. RH strains ofToxoplasmatachyzoites were used in the present study. Twenty-five female rats were infected with the parasite while 25 other rats were as the control group that received normal saline. Zero-, 5-, 7-, 10-, and 45-day postinfection (DPI) blood samples were taken. Some parameters related to oxidant and antioxidants such as antioxidant enzymes, malondialdehyde, and total antioxidant capacity were measured. On day 7 after infection, GPX activity and GSH level were significantly increased and in the mentioned day the amount of total antioxidant capacity was significantly reduced. In other cases, there were no significant differences between the groups in different days. Overall, based on the results it seems that, on day 7 after infection, in infected rats responses to oxidative stress were triggered and led to decrease of total antioxidant capacity. Furthermore, glutathione was increased to cope with stress. It seems that probably antioxidant defense system entered the infection to the chronic phase and changed the parasites stage.

scholarly journals Pengaruh pemberian buah naga merah, jambu biji merah, dan kombinasinya terhadap kapasitas antioksidan total dan kadar malondealdehid pada remaja perokok

2020 ◽  
Vol 9 (1) ◽  
pp. 53-60
Author(s):  
Mohammad Zainul Ma'arif ◽  
Suradi Suradi ◽  
Sugiarto Sugiarto
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
The Body ◽  
Smoking Habits ◽  
Control Group ◽  
Male Adolescent ◽  
Dragon Fruit ◽  
Dpph Method ◽  
Total Antioxidant ◽  
The Mean

Background: Smoking habits enhance free radical in the body, which oxidize lipids to form malondialdehyde. One group that is vulnerable to smoking is adolescents. Red dragon fruit and red guava have the possibility as functional foods due to their vitamin and phytochemical content, which have antioxidant effects. This is an opportunity to prevent oxidative stress among adolescent smokers.Objectives: This study aimed to analyze the effect of red dragon fruit, red guava, and its combination to total antioxidant capacity (TAC) and malondialdehyde (MDA) levels among adolescent mild smokers.Methods: This research was an experimental study with a randomized pre-test and post-test control group design. Forty-eight subjects of male adolescent aged 16-17 years with mild smoking habits were divided into four groups: the control group (K), the intervention group with 200 grams of red dragon fruit (P1), 200 grams of red guava (P2), and the combination of both (100 grams red dragon fruit + 100 grams red guava) (P3), for 14 days. TAC examination used the DPPH method and MDA levels using the TBARS method were measured before and after the intervention.Results: After 14 days of intervention, the mean TAC was changed by -0,10±0,76 in the K group, 0,97±1,62 in the P1 group, 0,74±1,39 in the P2 group, and 0,74±1,29 in the P3 group. But the changes of TAC in all intervention groups were not significantly different from the control group (p>0,05). The mean MDA level changed by 0,229±0,371 in the K group, decreased by -0,654±0,922 in the P1 group, -0,592±0,818 in the P2 group, and -1,166±0,670 in the P3 group. The mean difference of the MDA level in all intervention groups was significantly different from the control group (p<0,05).Conclusion: Consumption of red dragon fruit, red guava, or a combination of red dragon fruit and red guava can reduce malondialdehyde levels significantly among male adolescent mild smokers. Whereas the total antioxidant capacity was changed but not significant.

scholarly journals PO-198 Effect of aerobic exercise on antioxidation ability of mice kidneys

2018 ◽  
Vol 1 (4) ◽  
Author(s):  
Sisi He ◽  
Haitao Wang
Keyword(s):  
Aerobic Exercise ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Kidney Tissue ◽  
Exercise Group ◽  
Control Group ◽  
Adult Group ◽  
Total Antioxidant ◽  
The Difference ◽  
Control Group Group

Objective To study the total antioxidant capacity of the kidney in the Aged mice Total Antioxidant Capacity(T-AOC), Malondialdehyde (MDA) and superoxide dismutase (SOD) expression level by aerobic exercise. Objective to explore the effect of aerobic exercise on antioxidation ability of mice. Methods 30 Male C57BL/6 strain mice were randomly divided into adult group ( group CC) in the 13 months old, in the control group (Group C), 5 months of quiet feeding, the aerobic exercise group (Group E) ran for 5 months, 10 per group, and the adult group (group CC) compared with the control group (Group C), The Analysis control Group (Group C) was compared with the aerobic exercise group (Group E). After 5 months of Treadmill running in Group E, Determination of antioxidant index of each group. The statistical method is processed by SPSS20.0 statistic software, the experimental data is expressed by the mean ± standard deviation, the two mean numbers are analyzed by independent sample t test, and the difference of (p<0.05) is statistically significant. Results T-AOC of adult Group was (0.99±0.13) U/mg Prot, control group was (0.27±0.07) u/mg prot, aerobic exercise group (0.39±0.10) U/mg prot, adult group SOD as (46.32± 1.84) U/mg, control Group (29.71±7.64) U/mg, aerobic exercise group (39.85±5.83) U/mg, adult group MDA (8.15±0.16) nmol/mg, control Group (8.83±0.26) nmol/mg, The aerobic exercise group was (6.31±1.23) nmol/mg, the adult group was SOD/MDA (5.69±0.32), the control group was (3.38±0.9) and the aerobic exercise group was (6.55±1.08). Compared with the adult group, the control group T-AOC and SOD decreased significantly, MDA significantly increased, the difference was very significant (p<0.01), sod/mda significantly decreased, SOD/MDA difference was significant (p<0.01). Compared with the control group, the aerobic exercise group T-AOC and SOD obviously increased, MDA significantly decreased, the difference was significant (p<0.01), SOD/MDA increased significantly, the difference was very significant (p<0.01). Conclusions To sum up, with the increase of the age of mice MDA expression is increased, the 18 months old SOD expression is lower than 13 months old, indicating that the antioxidant capacity with the increase in age,  The results of this study are consistent with the results of previous scholars. The results show that aerobic exercise has some antioxidant effect, and aerobic exercise can decrease expression of kidney tissue SOD, raise the expression of kidney tissue T-AOC, increase the expressionof kidney tissue, increase the expression  of SOD/MDA, and reduce the oxidative stress of kidney to some extent,  So as to protect the role of the kidney, aerobic exercise and kidney-related mechanisms also need our further study.

scholarly journals Pengaruh Pemberian Jus Bit (Beta Vulgaris L) Selama Latihan Terhadap Kadar Malondialdehide Dan Status Antioksidan Atlet

2019 ◽  
Vol 3 (2) ◽  
pp. 119
Author(s):  
Fajar Apollo Sinaga ◽  
Rosmaini Hasibuan ◽  
Marsal Risfandi ◽  
Deni Rahman Marpaung ◽  
Jumadin IP ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Beta Vulgaris ◽  
Total Antioxidant Capacity ◽  
Control Group ◽  
Total Antioxidant ◽  
Randomized Control

Aktifitas fisik berat dapat menyebabkan peroksidasi lipid ditandai peningkatan kadar malondialdehyde (MDA) dan penurunan kadar antioksidan yang dapat menurunkan performance dan kesehatan atlet. Peroksidasi lipid dapat dicegah atau dikurangi dengan pemberian antioksidan. Bit (Beta Vulgaris L) mengandung berbagai jenis antioksidan alami tetapi belum pernah diteliti khasiatnya untuk mengurangi peroksidasi lipid yang dipicu oleh aktifitas fisik. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian jus bit selama latihan terhadap peroksidasi lipid dan status antioksidan pada saat melakukan aktifitas fisik maksimal. Jenis penelitian yang dilakukan adalah penelitian eksperimental dengan rancangan penelitian Randomized Control Group Pretest-Postest Design. Penelitian dilaksanakan di Stadion  Unimed dan Laboratorium Terpadu Fakultas Kedokteran USU. Sampel penelitian adalah mahasiswa Ilmu Keolahragaan sebanyak 30 orang  yang memenuhi kriteria. Pretest dilakukan dengan memeriksa kadar MDA, Total Antioxidant Capacity (TAC) dan VO2max. Selanjutnya sampel dibagi atas 2 kelompok  (P1=15, P2=15). Selama menjalani program latihan  kelompok P1 diberi Jus Bit 300 ml 1x1 hari, sedangkan P2 diberi sebagai kontrol. Kemudian semua sampel melakukan aktifitas fisik maksimal dengan menggunakan bleep test, dan kembali dilakukan pemeriksaan kadar MDA, TAC dan VO2max. Hasil penelitian menunjukkan terdapat penurunan kadar MDA serta peningkatan kadar TAC dan VO2max pada kelompok pelatihan yang diberi jus bit 300ml dibandingkan dengan kelompok kontrol (p<0,05). Kesimpulan penelitian adalah pemberian jus bit selama latihan dapat menurunkan kadar MDA serta meningkatkan kadar TAC dan VO2max atlet. Kata kunci: Jus Bit, Malondialdehyde, Antioksidan, Aktivitas Fisik Maksimal, Latihan

scholarly journals Effect of Curcumin on Oxidative Stress in a Model of Turpentine Induced Acute Experimental Inflammation

2017 ◽  
Vol 45 (1) ◽  
pp. 65-74 ◽  
Author(s):  
Andrei CONEAC ◽  
Meda Sandra ORASAN ◽  
Daniel Corneliu LEUCUTA ◽  
Nicoleta DECEA ◽  
Miuta FILIP ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
High Performance ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Total Antioxidant ◽  
Experimental Inflammation ◽  
Group Ii

Curcumin, a natural phenolic compound is an anti-tumor agent with anti-inflammatory and anti-oxidant properties. The aim of this research was to evaluate oxidative stress levels, the antioxidant activity and Curcumin concentrations by high performance liquid chromatography (HPLC) in an acute experimental inflammation induced by Turpentine oil (intramuscular 0.6 mg kg-1 body weight) and to compare a prophylactic versus a therapeutic regimen of Curcumin (oral suspension of 150 mg Curcumin kg-1 rat weight). Sixteen adult male Wistar rats were assigned to four groups: Control, Group I (Curcumin only), Group II (Curcumin administration, then induced inflammation after 1 hour) and Group III (induced inflammation then Curcumin administration after 2 hours). Oxidative stress was assessed by measuring serum malondialdehide and carbonylated proteins, while systemic and local total antioxidant capacity was determined by ABTS. Local tissue changes (muscle, kidney, liver) were analysed using histopathology. Results showed that acute inflammation significantly increased lipid peroxidation in Groups II and III compared to Control and Group I. A significantly reduced total antioxidant capacity (ATBS) was present in serum and kidney in Group II, also in muscle and kidney in Group III. ABTS levels were significantly increased only in the liver tissue of the animals in Groups II and III with induced inflammation as compared to Group I. This study proved the potential of Curcumin in reducing oxidative stress in both prophylactic and therapeutic regimens.

Adequate or elevated dietary folate does not ameliorate the reduced antioxidant capacity induced by vitamin B12 deficiency in aged rats

2020 ◽  
Vol 90 (3-4) ◽  
pp. 239-248
Author(s):  
Natalia Úbeda ◽  
Teresa Partearroyo ◽  
Gregorio Varela-Moreiras
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Control Diet ◽  
Elevated Serum ◽  
Control Group ◽  
Vitamin B ◽  
Aged Rats ◽  
Sprague Dawley ◽  
Deficient Diet ◽  
Total Antioxidant

Abstract. Folate could have an antioxidant role but also may be detrimental under vitamin B12 deficiency. The aim was to investigate the effect of different dietary folic acid (FA) levels, on oxidative stress in B12 induced deficient aged rats. Thirty-five male aged Sprague–Dawley rats, were fed either a vitamin B12 deficient (n = 27) or a control diet (n = 8) during eight weeks. Then, animals were divided into four groups: B12 and FA deficient diet (DBDF), B12 deficient diet and FA control diet (DBCF), B12 deficient diet and FA supplemented diet (DBSF), and control diet (CBCF) for a 30 days period. Methionine metabolism and antioxidant status were evaluated. Both vitamins deficiencies elevated serum homocysteine (Hcy) (7.7 vs. 4.3 μmol/L, p < 0.05) and reduced S-adenosylmethionine hepatic content (283.7 vs. 581.9 μg/g protein, p < 0.05), the total antioxidant capacity (155.7 vs. 189.3 μmol/L, p < 0.05), glutathione (GSH) (120.5 vs. 419.9 μg/mg protein, p < 0.05) and oxidized glutathione (0.9 vs. 2.6 μg/mg protein, p < 0.05) compared to control. Activities of glutathione peroxidase and glutathione reductase enzymes or damage to macromolecules were unaffected. Adequate or elevated dietary FA in B12 deficiency rats decreased Hcy (5.7 and 6.3 μmol/L, respectively) and increased total antioxidant capacity (189.8 and 192.6 μmol/L, respectively) to values similar to control group, whereas GSH concentration was significantly lower than control (209.1 and 208.0 μg/mg protein respectively, p < 0.05). In conclusion, in a vitamin B12 deficiency status, adequate or elevated FA prevented impairment in one-carbon metabolism, but does not fully reverse the decrease in antioxidant capacity.

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