scholarly journals Transcriptome Analysis Reveals Multiple Effects of Nitrogen Accumulation and Metabolism in the Roots, Shoots, and Leaves of Potato (Solanum Tuberosum L.)

2021 ◽  
Author(s):  
heng guo ◽  
xiuqin pu ◽  
hao jia ◽  
yun zhou ◽  
guangji ye ◽  
...  
Keyword(s):  
Solanum Tuberosum L ◽  
Sufficient Conditions ◽  
Gene Families ◽  
Potato Cultivars ◽  
Nitrogen Accumulation ◽  
N Accumulation ◽  
Kegg Pathways ◽  
Leaf Chlorophyll ◽  
New Ideas ◽  
Pigment Genes

Abstract Nitrogen (N) is a major element and fundamental constituent of grain yield. N fertilizer plays an essential role in the roots, shoots, and leaves of crop plants. Here, we obtained two N-sensitive potato cultivars. The plants were cultivated in the pots using N-deficient and N-sufficient conditions. Crop height, leaf chlorophyll content, dry matter, and N-accumulation significantly decreased under N-deficient conditions. Furthermore, we performed a comprehensive analysis of the phenotype and transcriptome, GO terms, and KEGG pathways. We used WGCNA of co-expressed genes, and 116 differentially expressed hub genes involved in photosynthesis, nitrogen metabolism, and secondary metabolites to generate 23 modules. Among those modules, six NRT gene families, four pigment genes, two auxin-related genes, and two energy-related genes were selected for qRT-PCR validation. Overall, our study demonstrates the co-expressed genes and potential pathways associated with N transport and accumulation in potato cultivars' roots, shoots, and leaves under N-deficient conditions. Therefore, this study provides new ideas to conduct further research on improving nitrogen use efficiency in potatoes.

Physiological and growth responses of potato cultivars to heat stress

Botany ◽  
2018 ◽  
Vol 96 (12) ◽  
pp. 897-912 ◽  
Author(s):  
Ruimin Tang ◽  
Suyan Niu ◽  
Guodong Zhang ◽  
Guanshui Chen ◽  
Muhammad Haroon ◽  
...  
Keyword(s):  
Heat Stress ◽  
Chlorophyll Content ◽  
Plant Height ◽  
Solanum Tuberosum L ◽  
Potato Cultivars ◽  
Stress Conditions ◽  
Correlated Response ◽  
Growth Responses ◽  
Leaf Chlorophyll Content ◽  
Leaf Chlorophyll

Climate warming is subjecting plants to heat stress, which can affect their physiological processes thereby impacting their growth, development, and productivity. Potato (Solanum tuberosum L.) is a staple food worldwide, but potato crops are very sensitive to heat stress. We have studied the effects of heat stress on the leaf chlorophyll content, plant growth, and tuber yield of 55 commercial potato cultivars in clonal tests under heat-stress conditions [HS; 35 °C (day), 28 °C (night)] and control (non-stress) conditions [CK; 22 °C (day), 18 °C (night)]. The potato cultivars varied in their response to heat stress. Overall, heat stress reduced leaf size, increased the SPAD index values for leaf chlorophyll by up to 65%, and increased plant height by 64%, but severely reduced (by 93%) the mass of the largest tuber. The HS:CK SPAD ratios positively correlated with the HS:CK plant height ratio, mass of the largest tuber under heat stress, and the HS:CK ratio for mass of the largest tuber. Potato cultivars displayed a correlated response to heat stress for their leaf chlorophyll content, plant height, and tuber mass. We have identified the most heat-tolerant and heat-susceptible cultivars for these traits. Under heat-stress conditions, potato cultivars tend not to show as much reduction in tuber mass if the plants have greater increases in leaf chlorophyll content and plant height.

scholarly journals Production high class of micro tuber potato seeds (Solanum tuberosum L.). by Using tissue culture technique

2009 ◽  
Vol 3 (2) ◽  
pp. 99-106
Author(s):  
A.A. Al-jibouri ◽  
A.A. Al-salhay
Keyword(s):  
Tissue Culture ◽  
Solanum Tuberosum L ◽  
Culture Technique ◽  
Potato Cultivars ◽  
Condition Numbers ◽  
Mass Propagation ◽  
High Class ◽  
Dormancy Period ◽  
Elisa Technique

The aim of this investigation was produced micro tubers of four potato cultivars Premiere, Bintje, Estima and Escort in vitro. Apical meristems (0.2-0.4 mm) of potato cultivars were excised and cultured on nutrient medium and incubated at 24±2 Cº and 1000 lux light intensity for 16 hrs per day. The developing plantlets were examined serological by using ELISA technique to eliminate the viral infected plantlets. The virus-free plantlets were chopped into pieces with single bud and re cultured on fresh medium for mass propagation. For micro tubers formation in test tubes, the cultures were transferred to another medium containing a high percent of sucrose (60g/L) with different concentrations of kinetin; the cultures were incubated under 16±2 Cº and 8 hrs photoperiod. The plantlets formed micro tubers after 8-10 weeks from culturing. The results showed significant differences among cultivar’s in their response to in vitro culture and micro tubers formation. The results also showed that the kinetin concentration had significant effect on micro tubers, and 1mg/l kinetin concentration was the best. The micro tubers were stored for 10 week at 4Cº to break down the dormancy period, and gave 100% germination under nursery condition. Numbers of tubers derived from micro tubers and normal tubers of these cultivars were compared at the end of season.

scholarly journals Genome-Wide Association Mapping of Late Blight Tolerance Trait in Potato (Solanum tuberosum L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Fang Wang ◽  
Meiling Zou ◽  
Long Zhao ◽  
Zhiqiang Xia ◽  
Jian Wang
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Potato Breeding ◽  
Genome Wide Association Study ◽  
Solanum Tuberosum L ◽  
Potato Cultivars ◽  
Genome Wide Association ◽  
Genome Wide ◽  
Population Structure Analysis ◽  
A Genome

Uncovering the genetic basis and optimizing the late blight tolerance trait in potatoes (Solanum tuberosum L.) are crucial for potato breeding. Late blight disease is one of the most significant diseases hindering potato production. The traits of late blight tolerance were evaluated for 284 potato cultivars to identify loci significantly associated with the late blight tolerance trait. Of all, 37 and 15 were the most tolerant to disease, and 107 and 30 were the most susceptible. A total of 22,489 high-quality single-nucleotide polymorphisms and indels were identified in 284 potato cultivars. All the potato cultivars were clustered into eight subgroups using population structure analysis and principal component analysis, which were consistent with the results of the phylogenetic tree analysis. The average genetic diversity for all 284 potato cultivars was 0.216, and the differentiation index of each subgroup was 0.025–0.149. Genome-wide linkage disequilibrium (LD) analysis demonstrated that the average LD was about 0.9 kb. A genome-wide association study using a mixed linear model identified 964 loci significantly associated with the late blight tolerance trait. Fourteen candidate genes for late blight tolerance traits were identified, including genes encoding late blight tolerance protein, chitinase 1, cytosolic nucleotide-binding site–leucine-rich repeat tolerance protein, protein kinase, ethylene-responsive transcription factor, and other potential plant tolerance-related proteins. This study provides novel insights into the genetic architecture of late blight tolerance traits and will be helpful for late blight tolerance in potato breeding.

scholarly journals Effects of nitrogen on yield and chlorophyll of potato (Solanum tuberosum L.) cultivars

1970 ◽  
Vol 38 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Semiha Güler
Keyword(s):  
Solanum Tuberosum ◽  
Tuber Yield ◽  
Solanum Tuberosum L ◽  
Total Yield ◽  
Correlation Coefficients ◽  
Tuber Number ◽  
Tuber Weight ◽  
Nitrogen Rate ◽  
Leaf Chlorophyll ◽  
Nitrogen Rates

Effects of nitrogen on the leaf chlorophyll (chl), yield and yield attributing characters of potato as tuber number and mean tuber weight were studied. Correlation coefficients between the investigated characters were determined. Five nitrogen rates (0, 150, 200, 250 and 300 kg/ha) and four potato cultivars (Burren, Slaney, Anna and Emma) were used in the study. First, second, third class tuber yields and total tuber yield, tuber number per plant, and leaf chl were significantly influenced by both nitrogen rate and cultivar, whereas mean tuber weight was affected only by cultivar. Maximum total yield was obtained at 200 kg N/ha. There was significant linear relationship between leaf chl and N applied (R2=0.91). There were significant correlations between chl and yield and yield related characters. Total yield significantly correlated with leaf chl. Correlations between first class yield and total yield as well as total yield and tuber number per plant were highly significant.   Key words: Potato; Solanum tuberosum L.; Leaf chlorophyll; Nitrogen; Yield DOI: 10.3329/bjb.v38i2.5141 Bangladesh J. Bot. 38(2): 163-169, 2009 (December)  

Sowing time and tillage practice affect chickpea yield and nitrogen fixation. 2. Nitrogen accumulation, nitrogen fixation and soil nitrogen balance

1996 ◽  
Vol 36 (6) ◽  
pp. 701 ◽  
Author(s):  
CP Horn ◽  
RC Dalal ◽  
CJ Birch ◽  
JA Doughton
Keyword(s):  
Nitrogen Fixation ◽  
N2 Fixation ◽  
N Balance ◽  
Grain Legume ◽  
Nitrogen Accumulation ◽  
Soil Nitrate ◽  
Soil N ◽  
Tillage Practice ◽  
Sowing Time ◽  
N Accumulation

Following long-term studies at Warra, on the western Darling Downs, chckpea (Cicer anetinum) was selected as a useful grain legume cash crop with potential for improvement of its nitrogen (N) fixing ability through management. This 2-year study examined the effect of sowing time and tillage practice on dry matter yield, grain yield (Horn et al. 1996), N accumulation, N2 fixation, and the subsequent soil N balance. Generally, greater N accumulation resulted from sowing in late autumn-early winter (89-117 kg N/ha) than sowing in late winter (76-90 kg N/ha). The amount of N2 fixed was low in both years (15-32 kg N/ha), and was not significantly affected by sowing time or tillage. The potential for N2 fixation was reduced in both years due to high initial soil nitrate levels and low total biomass of chickpea because of low rainfall. Nitrogen accumulation by grain was higher under zero tillage (ZT) than conventional tillage (CT) for all sowing times, and this affected the level of grain N export. The consequence of low N2 fixation and high N export in chickpea grain was a net loss of total soil N, (2-48 kg N/ha under CT and 22-59 kg N/ha under ZT). Management practices to ensure larger biomass production and lower soil nitrate-N levels may result in increased N2 fixation by chickpea and thus a positive soil N balance.

scholarly journals Wheat yield in the Cerrado as affected by nitrogen fertilization and inoculation with Azospirillum brasilense

2017 ◽  
Vol 52 (9) ◽  
pp. 794-805 ◽  
Author(s):  
Fernando Shintate Galindo ◽  
Marcelo Carvalho Minhoto Teixeira Filho ◽  
Salatiér Buzetti ◽  
José Mateus Kondo Santini ◽  
Cleiton José Alves ◽  
...  
Keyword(s):  
Grain Yield ◽  
Azospirillum Brasilense ◽  
Wheat Yield ◽  
Urease Inhibitor ◽  
N Accumulation ◽  
Leaf Chlorophyll ◽  
Spike Number ◽  
Tillage System ◽  
Irrigated Wheat ◽  
N Rates

Abstract: The objective of this work was to evaluate the effect of inoculation with Azospirillum brasilense, associated with N rates and sources, in soil of the Cerrado (Brazilian savanna), on the grain yield of irrigated wheat (Triticum aestivum). The experiment was carried out under a no-tillage system in a Typic Haplustox. The experimental design was randomized complete blocks with four replicates, in a 2x5x2 factorial arrangement: two N sources (urea and urea with NBPT urease inhibitor); five N rates applied as topdressing (0, 50, 100, 150, and 200 kg ha-1); and with or without seed inoculation with A.brasilense. The increase in the N rates positively affected spike length, number of spikelets and of grains per spike, number of spikes per meter, N accumulation in the straw, leaf chlorophyll content, and grain yield of irrigated wheat, regardless of the use of NBPT urease inhibitor with conventional urea. Singly, inoculation with A.brasilense does not affect production components and grain yield, despite the increase in N content in wheat straw. The inoculation with A.brasilense, associated with the application of 140 kg ha-1 N, provides the highest grain yield of irrigated wheat cropped after corn in low-altitude Cerrado.

scholarly journals The Leaf Wettability of Various Potato Cultivars

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 504
Author(s):  
Ewa Papierowska ◽  
Jan Szatyłowicz ◽  
Stanisław Samborski ◽  
Joanna Szewińska ◽  
Elżbieta Różańska
Keyword(s):  
Contact Angle ◽  
Sessile Drop ◽  
Leaf Surface ◽  
Solanum Tuberosum L ◽  
Fungal Disease ◽  
Potato Cultivars ◽  
Potato Leaf ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
Leaf Wettability

Leaf wettability has an impact on a plant’s ability to retain water on its leaf surface, which in turn has many environmental consequences. In the case of the potato leaf (Solanum tuberosum L.), water on the leaf surface may contribute to the development of a fungal disease. If fungal disease is caused, this may reduce the size of potato harvests, which contribute significantly to meeting global food demand. The aim of this study was to assess the leaf wettability of five potato cultivars (i.e., Bryza, Lady Claire, Rudawa, Russet Burbank, Sweet Caroline) in the context of its direct and indirect impact on potato yield. Leaf wettability was assessed on the basis of contact angle measurements using a sessile drop method with an optical goniometer. For Bryza and Rudawa cultivars, which showed, respectively, the highest and the lowest contact angle values, light microscopy as well as scanning electron microscopy analyses were performed. The results of the contact angle measurements and microscopic image analyses of the potato leaf surfaces indicated that the level of wettability was closely related to the type of trichomes on the leaf and their density. Therefore, higher resistance of the Rudawa cultivar to biotic stress conditions could be the result of the presence of two glandular trichome types (VI and VII), which produce and secrete metabolites containing various sticky and/or toxic chemicals that may poison or repel herbivores.

scholarly journals In vitro Regeneration and Agrobacterium-mediated Transformation of Potato (Solanum tuberosum L.) Cultivars Grown in Mexico

2013 ◽  
Vol 22 (2) ◽  
pp. 93-105 ◽  
Author(s):  
Rose Onamu ◽  
Juan P Legaria ◽  
Jaime C Sahagún ◽  
José L Rodríguez ◽  
Joel N Pérez
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
In Vitro Regeneration ◽  
Potato Cultivars ◽  
Pcr Analysis ◽  
Nptii Gene ◽  
Histochemical Assay ◽  
Gus Histochemical Assay ◽  
Binary Plasmid

Prior to Agrobacterium-mediated genetic transformation in vitro regeneration protocol was established for three potato cultivars (Alfa, Cambray Rosa Morelos and Atlantic) grown in Mexico using leaf, node and internodal explants. Regeneration protocol was developed with or without the intervention of callus. Two potato cultivars, namely, Cambray Rosa Morelos and Alpha were transformed using Agrobacterium tumefaciens strain LBA4404 harboring binary plasmid pBI121 containing the GUS and nptII genes. GUS histochemical assay and PCR analysis were conducted on rooted shoots grown in media without hormones but supplemented with antibiotics. Transformed shoots tested positive through GUS histochemical assay and integration of nptII gene was confirmed by PCR analysis DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14193 Plant Tissue Cult. & Biotech. 22(2): 93-105, 2012 (December)

Effect of soybean population density on soybean yield, nitrogen accumulation and residual nitrogen

1988 ◽  
Vol 28 (1) ◽  
pp. 99 ◽  
Author(s):  
MJ Blumenthal ◽  
VP Quach ◽  
PGE Searle
Keyword(s):  
Population Density ◽  
Grain Yield ◽  
Dry Matter ◽  
Nitrogen Accumulation ◽  
Soil N ◽  
Population Densities ◽  
N Accumulation ◽  
Soybean Yield ◽  
Residual Nitrogen ◽  
Residual N

The effect of soybean population density on soybean yield, nitrogen accumulation and residual nitrogen was examined at Camden, N.S.W. (34�S.). In the first experiment, treatments were soybeans (cv. Ransom) at 50, 100, 200 and 400 x 103 plants ha-1; maize (cv. XL66); and a weed-free fallow. Total dry matter yields of tops and grain yields were highest at 200x 103 plants ha-1 (6214 and 3720 kg ha-1, respectively). The yield component most affected by population density was number of branches per plant, with values decreasing with increasing population density. The proportion of unfilled pods was highest at the highest population density. Total nitrogen (N) accumulation in the tops and in the grain was also at a maximum at 200x 103 plants ha-1. The rate of dry matter accumulation declined during pod filling at all population densities. N accumulation continued at high rates throughout the growing season except in the 400x 103 plants ha-1 population. There was a trend for residual dry matter and N in residues to increase with increasing population density. After grain and forage harvest of the first experiment, a crop of wheat (cv. Kite) was sown over the whole area to determine residual N available at anthesis and at maturity (experiment 2). The values of N accumulation in the wheat at maturity were 24 kg N ha-l for the maize treatment, 40-60 kg N ha-l for the soybean treatments and 69 kg N ha-1 for the fallow treatment. Grain yield and grain N followed the pattern of dry matter production and N accumulation at final harvest. The data suggest that soybean depletes soil N to a lesser extent than does maize. For the soybean treatments, there was a trend of increasing residual N at the 3 highest population densities (40-60 kg N ha-1). This was probably a result of an increase in N in leaf fall and in decaying tops and roots at the highest population density. The high value (57 kg N ha-l) at the lowest population density may be due to soybean plants at this density not using as much soil N as the other soybean treatments. No benefit in residual N was gained from planting soybeans at a density beyond the optimum for grain yield when residues were removed by forage harvesting.

Nitrogen use efficiency characteristics of commercial potato cultivars

2004 ◽  
Vol 84 (2) ◽  
pp. 589-598 ◽  
Author(s):  
B. J. Zebarth ◽  
G. Tai ◽  
R. Tarn ◽  
H. de Jong ◽  
P. H. Milburn
Keyword(s):  
Nitrogen Use Efficiency ◽  
Dry Matter ◽  
Potato Cultivars ◽  
Utilization Efficiency ◽  
Dry Matter Accumulation ◽  
N Accumulation ◽  
Nitrogen Use ◽  
N Supply ◽  
Commercial Potato ◽  
Use Efficiency

One approach for reducing the contribution of potato (Solanum tuberosum L.) production to nitrate contamination of groundwater is to develop cultivars which utilize N more efficiently. In this study, variation in N use efficiency (NUE; dry matter production per unit crop N supply) characteristics of 20 commercial potato cultivars of North American and European origin were evaluated in 2 yr. Cultivars were grown with or without application of 100 kg N ha-1 as ammonium nitrate banded at planting. The recommended within-row spacing was used for each cultivar and no irrigation was applied. Plant dry matter and N accumulation were determined prior to significant leaf senescence. Crop N supply was estimated as fertilizer N applied plus soil inorganic N measured at planting plus apparent net soil N mineralization. Nitrogen use efficiency decreased curvilinearly with increasing crop N supply. Nitrogen use efficiency was lower for early-maturing cultivars compared to mid-season and late-maturing cultivars. A curvilinear relationship was obtained between plant dry matter accumulation and plant N accumulation using data for all cultivars. Deviations from this relationship were interpreted as variation in N utilization efficiency (NUtE; dry matter accumulation per unit N accumulation). Significant differences in NUtE were measured among cultivars of similar maturity. Nitrogen uptake efficiency (NUpE; plant N content per unit crop N supply) and soil nitrate concentration measured at plant harvest were uniformly low for all cultivars when crop N supply was limited, but varied among cultivars when N was more abundant. This suggests that potato cultivars vary more in terms of N uptake capacity (plant N accumulation in the presence of an abundant N supply) than in terms of NUpE. Key words: Solanum tuberosum, N mineralization, dry matter accumulation, N accumulation, N utilization efficiency

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