scholarly journals The Expression of MMP-2 on the Pressure Side During Tooth Movement

2021 ◽  
Author(s):  
Jing Zhang ◽  
Qiuqiu Chen ◽  
Fuxiang Luo ◽  
Hong Liu
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Immunohistochemical Analysis ◽  
Matrix Metalloproteinase 2 ◽  
Maxillary Incisor ◽  
Control Group ◽  
Root Absorption ◽  
Traction Device ◽  
The Right ◽  
Hematoxylin Eosin

Abstract Objectives: To explore the effects of matrix metalloproteinase-2 (MMP-2) on the development of orthodontic root resorption in rats and to provide a theoretical basis for prevention and treatment of root resorption in clinical diagnosis.Methods: Thirty-five 6-8-week-old male SD rats were randomly divided into 7 groups, each with 5 rats weighing approximately 200 g±20 g. An orthodontic traction device was applied between the right first molar and the maxillary incisor in the experimental rats, and a mesial tractive force of 60 g was applied. Five rats were used for the control group without orthodontic applications. After 0, 1, 3, 5, 7, 14, and 21 days, the maxillae were abducted, and immunohistochemical analysis was performed. We used hematoxylin eosin staining to observe root resorption. The expression of MMP-2 was also assessed by immunohistochemical staining. SPSS17.0 statistical software was used to process the data.Results: Forcing 3 d was observed in the formation of bone resorption lacunae, which were confined to the cementum. After that, it becomes more serious. The most serious damage in 7 days. In 14 d, root absorption was relieved, and osteogenic cells aggregated at the root absorption site. Root resorption lacuna visible dentin repair phenomenon. With the extension of the force application time, the expression of MMP-2 was raised gradually, reached the peak at 7 d, and then gradually declined, 21d tends to be stable, but still stronger than controls.Conclusion: MMP-2 is involved in the metabolism of extracellular matrix in the process of root absorption.

ROLE OF THE HYALURONAN-PRODUCING TENOSYNOVIUM IN PREVENTING ADHESION FORMATION DURING HEALING OF FLEXOR TENDON INJURIES

Hand Surgery ◽  
2012 ◽  
Vol 17 (01) ◽  
pp. 13-17 ◽  
Author(s):  
Masaharu Yagi ◽  
Yasuhiro Mitsui ◽  
Masafumi Gotoh ◽  
Naoto Sato ◽  
Kenji Yoshida ◽  
...  
Keyword(s):  
Flexor Tendon ◽  
Adhesion Formation ◽  
Immunohistochemical Analysis ◽  
Tendon Injuries ◽  
White Leghorn ◽  
Group I ◽  
Flexor Tendon Injuries ◽  
The Right ◽  
Hematoxylin Eosin

Flexor tendons of white Leghorn chickens (n = 25) were used for this study. One chicken was used as a normal control (no surgery), and the remaining 24 were used for experiments. After partial tendon-severing in both legs of 24 chickens, the right and the left leg were treated differently, thereby creating two groups: Group I, in which the tenosynovium was preserved, and Group II, in which the tenosynovium was removed. Hematoxylin-eosin staining was performed to observe adhesions; immunohistochemical analysis was used to localize HA. HA production was noted in granulation tissue invading between the tendon stumps in both groups; however, HA expression in the tenosynovium was observed only in Group I where adhesion formation was minimal. The HA-producing tenosynovium plays a crucial role in preventing adhesion formation in this model of flexor tendon injuries.

scholarly journals Comparison of Rate of Canine Retraction and Secondary Outcomes Associated With Conventional Fixed Orthodontic Treatment and Minimally Invasive Techniques (MOPs): A Randomized Control Trial

2020 ◽  
pp. 030157422096350
Author(s):  
Ajit Vikram Parihar ◽  
Shivam Verma ◽  
TP Chaturvedi ◽  
Naresh Kumar ◽  
A Kavin Prasanth ◽  
...  
Keyword(s):  
Orthodontic Treatment ◽  
Tooth Movement ◽  
Root Resorption ◽  
Control Group ◽  
P Value ◽  
Pain Level ◽  
Canine Retraction ◽  
Significant Difference ◽  
Secondary Outcomes ◽  
Anchorage Loss

Introduction: The objective of the study was to assess the rate of canine retraction and secondary outcomes associated with conventional fixed orthodontic treatment (CFO) and CFO with micro-osteoperforation (CFO + MOP), that is, anchorage loss, root resorption, vitality of tooth, pain and discomfort level during the procedure. Methods: A total of 16 patients with Class II Division 1 malocclusion who required upper first premolar extraction with lower non-extraction/single incisor extraction were divided into the test group (MOP) and positive control group (CFO + MOP) for a split-mouth study. Both maxillary canines were retracted with nickel–titanium (NiTi) closed coil springs. Patients were reviewed after 24 hours, 7 days, 28 days, and 4, 8, and 16 weeks to assess the rate of tooth movement, anchorage loss, root resorption, vitality of tooth, pain and discomfort level. Results: There was a statistically significant difference in the rate of tooth movement between the CFO and CFO + MOP groups after the first 4 weeks ( P-value = .026), whereas no statistically significant difference was observed at 8, 12, and 16 weeks ( P-value = .33, .99, and .08, respectively). In the CFO group, there was no statistically significant difference in tooth movement between different time intervals ( P-value > .05). There was no significant difference in root resorption between the groups. The pain level was higher in the MOP group in the first 24 hours ( P-value < .05) after the procedure. Later on, the difference in pain level between the groups was not significant ( P-value > .05). The vitality of retracted canines in both groups was healthy. Conclusion: The study recommends that the CFO + MOP procedure has substantial potential to be used as an adjunct to the routine mechanotherapy for faster tooth movement, as it may reduce the treatment time by half in the first 4 weeks after the MOP procedure. There are no potential differences in anchorage loss, tipping, vitality, and apical and lateral root resorption between the CFO and CFO + MOP groups. This trial was registered at Clinical Trial Registry, India.

scholarly journals Effect of supplementary zinc on orthodontic tooth movement in a rat model

2016 ◽  
Vol 21 (2) ◽  
pp. 45-50 ◽  
Author(s):  
Mohammad Sadegh Ahmad Akhoundi ◽  
Rezvaneh Ghazanfari ◽  
Shahroo Etemad-Moghadam ◽  
Mojgan Alaeddini ◽  
Azam Khorshidian ◽  
...  
Keyword(s):  
Rat Model ◽  
Tooth Movement ◽  
Root Resorption ◽  
Orthodontic Tooth Movement ◽  
Cumulative Effect ◽  
Study Groups ◽  
Model Material ◽  
Bone Homeostasis ◽  
Male Wistar Rats ◽  
Hematoxylin Eosin

ABSTRACT Introduction: Osteoclasts and osteoblasts are responsible for regulating bone homeostasis during which the trace element zinc has been shown to exert a cumulative effect on bone mass by stimulating osteoblastic bone formation and inhibiting osteoclastic bone resorption. Objective: The aim of the present study was to investigate the effects of zinc (Zn) on orthodontic tooth movement (OTM) in a rat model. Material and Methods: A total of 44 male Wistar rats were divided into four groups of 11 animals each and received 0, 1.5, 20 and 50 ppm Zn in distilled water for 60 days. In the last 21 days of the study, nickel-titanium closed coil springs were ligated between maxillary right incisors and first molars of all rats, and tooth movement was measured at the end of this period. Histological analysis of hematoxylin/eosin slides was performed to assess root resorption lacunae, osteoclast number and periodontal ligament (PDL) width. Results: Mean OTM was calculated as 51.8, 49.1, 35.5 and 45 µm in the 0, 1.5, 20 and 50 ppm zinc-receiving groups, respectively. There were no significant differences in neither OTM nor histological parameters among the study groups (p > 0.05). Conclusion: According to the results obtained in the current investigation, increase in supplementary zinc up to 50 ppm does not affect the rate of OTM neither bone and root resorption in rats.

scholarly journals Effect of Methotrexate Injection on Orthodontic Tooth Movement: An Experimental Study on Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Amin Golshah ◽  
Khaled Omidi ◽  
Nafiseh Nikkerdar ◽  
Hedaiat Moradpoor ◽  
Fatemeh Ghorbani
Keyword(s):  
Blood Vessels ◽  
Orthodontic Treatment ◽  
Tooth Movement ◽  
Root Resorption ◽  
Orthodontic Tooth Movement ◽  
Volume Ratio ◽  
Negative Control Group ◽  
Negative Control ◽  
Bone Volume ◽  
Control Group

Introduction. Knowledge about the effects of medications, vitamins, and various supplements on orthodontic tooth movement (OTM) is imperative for orthodontists. This study aimed to assess the effect of methotrexate (MTX) injection on OTM in rats. Materials and Methods. Twenty-eight male Wistar rats were randomized into four groups (n = 7). The first molar and central incisor were connected using a nickel-titanium (NiTi) coil spring with a 50 g load in each rat. The two experimental groups received 0.75 mg/kg and 1.5 mg/kg MTX, respectively, intraperitoneally for 21 days. The negative control group did not receive any injection and did not undergo orthodontic treatment. The positive control group underwent orthodontic treatment and received 0.9% saline (NaCl) injections for 21 days. All rats were sacrificed with chloroform inhalation after 21 days; their maxilla was resected, and the mean number of Howship’s lacunae, blood vessels, osteoclasts, and resorption lacunae was counted. The reduction in bone volume (bone volume to total volume ratio (BV/TV)) at the site of the maxillary molar was quantified by microcomputed tomography (micro-CT). Results. OTM, the number of osteoclasts, and the number of blood vessels significantly increased in rats treated with MTX ( P < 0.05 ). However, the increase in the number of Howship’s lacunae and resorption lacunae was not significant ( P > 0.05 ). Lower BV/TV in the MTX groups was in agreement with the increased number of osteoclasts. Conclusion. Injection of MTX can significantly increase OTM and decrease root resorption in rats.

scholarly journals Effects of Steroidal and Nonsteroidal Drugs on Tooth Movement and Root Resorption in the Rat Molar

2009 ◽  
Vol 79 (4) ◽  
pp. 715-726 ◽  
Author(s):  
Carmen Gonzales ◽  
Hitoshi Hotokezaka ◽  
Ken-Ichiro Matsuo ◽  
Tatsunori Shibazaki ◽  
Joseph H. Yozgatian ◽  
...  
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Three Dimensional ◽  
Negative Control Group ◽  
Treated Group ◽  
Negative Control ◽  
Control Group ◽  
High Dose ◽  
Experimental Control ◽  
Male Wistar Rats

Abstract Objective: To test the hypothesis that the administration of aspirin, acetaminophen, meloxicam, celecoxib, and prednisolone have no effect on root resorption and tooth movement. Materials and Methods: A mesial force of 50 g was applied to the left maxillary first molars of sixty 10-week-old male Wistar rats using nickel titanium closed coil springs attached to the cervical area of the incisors. The rats were randomly divided into 12 groups of 5 each. High and low doses of aspirin, acetaminophen, meloxicam, celecoxib, and prednisolone were administered via drinking water for 2 weeks. The experimental control group had tooth movement but received no drug. The negative control group received neither tooth movement nor drugs. The amount of tooth movement was measured on digitized lateral cephalometric radiographs. Rats were sacrificed after 2 weeks. Mesial and distal roots (distobuccal and distopalatal) were examined using scanning electron and three-dimensional (3D) scanning laser microscopes. The surface area, depth, volume, and roughness of the root resorption craters were measured. Results: When compared with experimental control rats, only prednisolone- and high-dose celecoxib-treated groups showed significantly less root resorption and less tooth movement. Although low dose celecoxib-treated group significantly decreased the tooth movement, root resorption was similar to the control group. Furthermore, resorption craters showed a smoother surface in the prednisolone-treated rats. Conclusions: The hypothesis was rejected. Administration of prednisolone and high-dose celecoxib reduces root resorption and interferes with tooth movement in rats. Both drugs may interfere in the arachidonic acid cascade depending on dose thresholds.

scholarly journals Ameloblastic Carcinoma in a 2-Year-Old Child: A Case Report and Review of the Literature

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Ngoc Bao Vu ◽  
Ngoc Tuyen Le ◽  
Risa Chaisuparat ◽  
Pasutha Thunyakitpisal ◽  
Ngoc Minh Tran
Keyword(s):  
Rare Case ◽  
Pediatric Patients ◽  
Root Resorption ◽  
Immunohistochemical Analysis ◽  
Incisional Biopsy ◽  
Review Of The Literature ◽  
Ameloblastic Carcinoma ◽  
Valuable Contribution ◽  
Radiographic Findings ◽  
The Right

Ameloblastic carcinoma (AC) is a rare malignant odontogenic tumor in pediatric patients, only 22 cases have been reported in literature since 1932. We present an extremely rare case in which AC occurred in a 2-year-old girl, who had a tumor in the right mandible. Radiographic findings showed a multilocular, poorly defined, and mixed radiolucent-radiopaque lesion in the region of teeth #84 to #85, with bone and tooth root resorption. Computed tomography revealed buccal cortex destruction, tumor infiltration of soft tissue, and enlarged nodes. Incisional biopsy showed histomorphological features of AC. Immunohistochemical analysis exhibited a positive result for Cytokeratin (CK) 19 and overexpression of p53 and Ki67. The patient underwent right hemimandibulectomy and neck dissection. The final pathology was consistent with the initial diagnosis of AC. The patient did not exhibit signs of recurrence or metastasis within 2 years postoperatively. Given the rarity of this disease and the age of the patient, this report constitutes a valuable contribution to the current literature.

scholarly journals Effects of Gradually Increasing Force Generated by Permanent Rare Earth Magnets for Orthodontic Tooth Movement

2006 ◽  
Vol 76 (6) ◽  
pp. 1004-1009 ◽  
Author(s):  
Ryo Tomizuka ◽  
Hiroyasu Kanetaka ◽  
Yoshinaka Shimizu ◽  
Akihiro Suzuki ◽  
Kaoru Igarashi ◽  
...  
Keyword(s):  
Rare Earth ◽  
Tooth Movement ◽  
Root Resorption ◽  
Orthodontic Tooth Movement ◽  
Male Rats ◽  
Control Group ◽  
Pathological Changes ◽  
Group I ◽  
Group Ii ◽  
Experimental Group

Abstract Objective: To examine the effects of gradually increasing force generated by permanent rare earth magnets for orthodontic tooth movement by using a novel experimental rat model and computer simulation. Materials and Methods: Fifty-five male rats (age 18 weeks) were used as animal experiments. Magnetic (experimental groups) or titanium (control group) cuboids (1.5 × 1.5 × 0.7 mm) were bonded to the lingual surface of the maxillary first molars. The initial distance between materials was 1.0 mm, generating 4.96 gf (experimental group I), or 1.5 mm, generating 2.26 gf (experimental group II). Tooth movement was measured and periodontal structures were observed with microfocus x-ray computed tomography radiographs. Results: The distance between the magnets decreased with time in experimental groups I and II (P &lt; .001), whereas there was no tooth displacement in the control group. Experimental group I showed rapid tooth movement in the initial phase followed by slower tooth movement. Experimental group II showed gradual tooth movement. Horizontal sections on microfocus computed tomgraphy radiographs revealed no pathological changes, such as root resorption, on the compressed side in the experimental groups. Conclusions: The initial light force and gradual increasing force in magnetic attractive force induced effective tooth movement in rats without inducing any pathological changes.

scholarly journals Inhibitory effect of baicalin on orthodontically induced inflammatory root resorption in rats

2020 ◽  
Vol 48 (9) ◽  
pp. 030006052095507
Author(s):  
Peng Lin ◽  
Xin-xing Guo ◽  
Yi-ling Wang ◽  
Zhu-liang Wei ◽  
Hai-yan Xin ◽  
...  
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Negative Control Group ◽  
Inhibitory Effect ◽  
Negative Control ◽  
Area Ratio ◽  
Control Group ◽  
Tnf Α ◽  
Significant Difference ◽  
Movement Distance

Objective This study investigated the inhibitory effect of baicalin on orthodontically induced inflammatory root resorption in rats. Methods Forty-five male Wistar rats were randomly divided into three groups of 15 rats each. Fifty grams of force was used to establish an orthodontic tooth movement model. Baicalin (40 mg/kg) was locally injected into rats in the baicalin group at 3-day intervals; concurrently, normal saline was injected into rats in the negative control group. On the 21st day after orthodontic treatment, the tooth movement distance and root resorption area ratio were measured. Histomorphology changes were observed by hematoxylin and eosin staining and immunohistochemistry. Results There was no significant difference in tooth movement distance between groups. The root resorption area ratio was significantly lower in the baicalin group than in the negative control group. Runx-2 expression was significantly higher in the baicalin group than in the negative control group, while tumor necrosis factor (TNF)-α expression was significantly lower in the baicalin group than in the negative control group. Conclusions Baicalin inhibits orthodontically induced inflammatory root resorption by enhancing the expression of Runx-2 and reducing the expression of TNF-α, but does not affect tooth movement distance.

scholarly journals Effect of Low Intensity Pulsed Ultrasound (LIPUS) on Tooth Movement and Root Resorption: A Prospective Multi-Center Randomized Controlled Trial

2020 ◽  
Vol 9 (3) ◽  
pp. 804 ◽  
Author(s):  
Tarek El-Bialy ◽  
Khaled Farouk ◽  
Terry D. Carlyle ◽  
William Wiltshire ◽  
Robert Drummond ◽  
...  
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Controlled Trial ◽  
Control Group ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Control Side ◽  
Before And After ◽  
Group 1

The aim of this study was to evaluate the possible effect of low intensity pulsed ultrasound (LIPUS) on tooth movement and root resorption in orthodontic patients. Twenty-one patients were included in a split-mouth study design (group 1). Ten additional patients were included with no LIPUS device being used and this group was used as the negative control group (group 2). Group 1 patients were given LIPUS devices that were randomly assigned to right or left side on upper or lower arches. LIPUS was applied to the assigned side that was obtained by randomization, using transducers that produce ultrasound with a pulse frequency of 1.5 MHz, a pulse repetition rate of 1 kHz, and average output intensity of 30 mW/cm2. Cone-beam computed tomography (CBCT) images were taken before and after treatment. The extraction space dimensions were measured every four weeks and root lengths of canines were measured before and after treatment. The data were analyzed using paired t-test. The study outcome showed that the mean rate of tooth movement in LIPUS side was 0.266 ± 0.092 mm/week and on the control side was 0.232 ± 0.085 mm/week and the difference was statistically significant. LIPUS increased the rate of tooth movement by an average of 29%. For orthodontic root resorption, the LIPUS side (0.0092 ± 0.022 mm/week) showed a statistically significant decrease as compared to control side (0.0223 ± 0.022 mm/week). The LIPUS application accelerated tooth movement and minimized orthodontically induced tooth root resorption at the same time.

The Effect of Amitriptyline and Sertraline on the Tooth Movement, Root Resorption and Alveolar Bone Remodeling After Load Application in Dogs

2021 ◽  
Author(s):  
Mehdi Rafiei ◽  
Sousan Sadeghian
Keyword(s):  
Bone Formation ◽  
Bone Remodeling ◽  
Alveolar Bone ◽  
Tooth Movement ◽  
Root Resorption ◽  
Load Application ◽  
Control Group ◽  
P Value ◽  
External Root Resorption ◽  
Alveolar Bone Remodeling

Introduction: Antidepressant drugs are the most commonly prescribed classes of pharmacologic agents in the public. The drugs have been shown to have a role in the regulation of bone cell function and as a result affecting the orthodontic tooth movements. The aim of this study was to determine the effect of Amitriptyline and Sertraline on the tooth movement, root resorption and alveolar bone remodeling after load application in dogs.   Materials and Methods: In this experimental study, 9 male dogs were randomly divided into three groups, first group sertraline, second group Amitriptyline and the control group (normal saline). A nickel titanium spring (200 gr) was used between second premolar and canine after 1st premolar extraction. After 2 months, the reduction of distance between 2nd premolar and canine was measured. The percentages of root resorption and bone formation were determined. The data were analyzed using repeated measures analysis at significance level of 0.05.   Results: In the three groups of amitriptyline, sertraline and control, the mean of teeth movement (p value = 0.483), external root resorption (p value = 0.608), total bone mineral density (p value = 0.078), bone formation percentage (p value = 0.616) and immature and lamellar bone formation (p value = 0.083), there was no statistical difference in any group and in premolar teeth Conclusions: The rate of tooth movement and the percentages of bone formation and root resorption in dogs decreased with systemic administration of amitriptyline and sertraline; although this reduction was not statistically significant in comparison with control group. 

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