scholarly journals The role of rejuvenated and adult forms of English oak (Quercus robur) in in vitro cultures

1999 ◽  
Vol 5 (1-2) ◽  
Author(s):  
Gy. Végvári ◽  
I. Balla ◽  
Z. Kothencz
Keyword(s):  
Chlorophyll Content ◽  
Cold Treatment ◽  
Dry Weight ◽  
In Vitro Cultures ◽  
Three Samples ◽  
Subculture Period ◽  
The Media ◽  
English Oak

In vitro plant material of clones (Q. robur) NL 100 A (adult) and NL 100 R (rejuvenated) received from Germany (A. Meier-Dinkel, 1995) were used in these experiments. WPM medium was used for the multiplication phase. Plantlets were subcultured monthly. Differences in quality and colour of the adult and rejuvenated cultures induced us to follow and compare the changes of mineral- and chlorophyll content and dry weight during the propagation phase. Mineral and chlorophyll content as well as dry weight were measured weekly on three samples during the subculture period. In the case of propagation rates we stated, they were similar around the year, but both clones had a high peak in April. Examining the cation-content, we detected that, the plantlets had a highest quantity of several elements during the 2nd and 3rd week of subculture. The iron content was the highest in the 1st week and after that it decreased continuously. It is supposed, that the content of iron is not enough in the media. The chlorophyll content of the rejuvenated clone was higher than that of the adult one. In the rooting experiments it was stated that, after one-week cold treatment the rooting ability was the best.  

Role of gibberellic acid in cotton fibre development

2002 ◽  
Vol 138 (3) ◽  
pp. 255-260 ◽  
Author(s):  
S. J. GOKANI ◽  
V. S. THAKER
Keyword(s):  
Gibberellic Acid ◽  
Fibre Length ◽  
Dry Weight ◽  
Cotton Fibre ◽  
Growth Phases ◽  
Fibre Development ◽  
The Media

Fibres of three cotton cultivars (Gossypium hirsutum H-4, H-8 and G. arboreum G. Cot-15) were analysed for growth in terms of fibre length and dry weight and endogenous gibberellic acid (GA3) content thrice during 1997–2000, at Rajkot. The development of cotton fibre was divided into four distinct growth phases but overlap between elongation and secondary thickening was considerable which suggests that both these phases are independent of each other. During fibre elongation, GA3 content remained low and increased after a decrease in the rate of fibre elongation in all three genotypes. The long staple cultivar (H-4) showed highest endogenous GA3 content followed by the middle one (H-8) and the short staple cultivar (G. Cot-15). In in vitro studies when GA3, NAA or GA3+NAA was supplemented to the media, increase in fibre length of the short staple cultivar was maximum, followed by the middle one and the long staple cultivar. Both in vivo and in vitro findings suggest that GA3 is one of the important factors that determine fibre length.

scholarly journals IN VITRO NITROGEN METABOLISM AND ORGANOGENESIS OF NICOTIANA TABACUM

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1121c-1121
Author(s):  
Victoria E. Rudolph ◽  
David W. Burger
Keyword(s):  
Solid Medium ◽  
N Uptake ◽  
Dry Weight ◽  
Inorganic N ◽  
Net Uptake ◽  
History Of ◽  
N Metabolism ◽  
The Media

The role of N metabolism in organogenesis and growth was studied using tobacco pith callus. Callus was cultured on a solid medium containing 10 μM (1.75 mg/l) IAA and 2 μM (0.43 mg/l) kinetin for 56 days. In the growth experiment, ratios of NH4+-N to NO3--N (0:60, 20:40, 30:30, 40:20 and 60:0 mM) were supplied by (NH4)2 SO4 and KNO3. Callus and media were analyzed for inorganic N. Callus supported by 30:30 and 40:20 media removed the highest amounts of NH4+-N and NO3--N from the media and exhibited organogenesis. Final dry weight was greatest in callus supported by the 30:30 medium. In the organogenesis experiment, the transfer history of the inoculum source affected N uptake, organogenesis and growth. Inorganic N was supplied by NH4NO3 and KNO3-. The net uptake of NH4+-N and NO3--N was lower in shoot-forming than in root-forming and non-organogenic callus subculture from 7-day-old stock cultures. The final pH of the medium supporting shoot-forming callus was lowest. Growth, on a dry weight basis, was lowest in shoot-forming callus. Callus subculture from 60-day-old stock cultures formed no shoots.

THE ROLE OF CYTOKININS AND ETHYLENE INHIBITORS ON LENTICEL HYPERTROPHY GENERATION AND ETHYLENE PRODUCTION IN IN VITRO CULTURES OF POPULUS EUPHRATICA OLIVIER

1995 ◽  
Vol 43 (4) ◽  
pp. 339-345 ◽  
Author(s):  
M.D. Lledó ◽  
M.B. Crespo ◽  
J.B. Amo-Marco
Keyword(s):  
Ethylene Production ◽  
Populus Euphratica ◽  
In Vitro Cultures ◽  
Nodal Segments ◽  
Ethylene Inhibitors ◽  
Se Spain ◽  
Ethylene Formation ◽  
Free Media

Populus euphratica Olivier is native to the Irano—Turanian areas (Middle East). Elche (Alicante province, SE Spain) is known to be its only European location. Nodal segments from root shoots were established in vitro in a Murashige and Skoog medium supplemented with several cytokinins. Ethylene inhibitors AgNO3 and CoCl2 were used in combination with kinetin. Hormone-free media supplemented with sucrose (20–60 mg 1−1) was also tested. Ethylene was measured by gas chromatography, and both the percentage of sprouting shoots and lenticel hypertrophy in cultures were recorded. Ethylene production was higher in cultures supplemented with cytokinins (especially with meta-topolin), with high sprouting percentages, and lenticel hypertrophy. In cultures supplemented with 6-benzylaminopurine or 6-(γ,γ,-dimethylallylamino)-purine, ethylene production was lower and explants looked unhealthy. Ethylene formation was inhibited in cultures supplemented with AgNO3 (1 mg 1−1), which also decreased percentage of sprouting buds and lenticel hypertrophy.

scholarly journals Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Essential Oils from AgNPs and AuNPs Elicited Lavandula angustifolia In Vitro Cultures

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 606 ◽  
Author(s):  
Aneta Wesołowska ◽  
Paula Jadczak ◽  
Danuta Kulpa ◽  
Włodzimierz Przewodowski
Keyword(s):  
Molecular Weight ◽  
Silver Nanoparticles ◽  
Essential Oils ◽  
Culture Media ◽  
In Vitro Cultures ◽  
Gas Chromatography Mass Spectrometry ◽  
Lavandula Angustifolia ◽  
Gold And Silver Nanoparticles ◽  
The Media

The aim of this study was to determine how the addition of gold and silver nanoparticles to culture media affects the composition of essential oils extracted from Lavandula angustifolia propagated on MS media with the addition of 10 and 50 mg·dm−3 of gold (24.2 ± 2.4 nm) and silver (27.5 ± 4.8 nm) nanocolloids. The oil extracted from the lavender tissues propagated on the medium with 10 mg·dm−3 AgNPs (silver nanoparticles) differed the most with respect to the control; oil-10 compounds were not found at all, and 13 others were detected which were not present in the control oil. The addition of AuNPs (gold nanoparticles) and AgNPs to the media resulted in a decrease of lower molecular weight compounds (e.g., α- and β-pinene, camphene, δ-3-carene, p-cymene, 1,8-cineole, trans-pinocarveol, camphoriborneol), which were replaced by those of a higher molecular weight (τ- and α-cadinol 9-cedranone, cadalene, α-bisabolol, cis-14-nor-muurol-5-en-4-one, (E,E)-farnesol).

scholarly journals Biosynthesis and characterization of silver nanoparticles using Ochradenus arabicus and their physiological effect on Maerua oblongifolia raised in vitro

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hassan O. Shaikhaldein ◽  
Fahad Al-Qurainy ◽  
Mohammad Nadeem ◽  
Salim Khan ◽  
Mohamed Tarroum ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Chlorophyll Content ◽  
Total Protein ◽  
Dry Weight ◽  
Shoot Length ◽  
Antioxidant Enzyme Activities ◽  
Fresh Weight ◽  
Wide Range ◽  
Shoot Number

Abstract Silver nanoparticles (AgNPs) are presently the most commonly generated engineered nanomaterials and are found in a wide range of agro-commercial products. The present study was designed to synthesize AgNPs biologically using Ochradenus arabicus leaves and investigate their effect on the morphophysiological properties of Maerua oblongifolia raised in vitro. Physicochemical methods (ultraviolet–visible spectroscopy, Fourier transform infrared spectroscopy, and transmission electron microscopy were performed for characterization and for obtaining microphotographs of the AgNPs. Shoots of M. oblongifolia (2–3 cm) grown in Murashige and Skoog medium supplemented with different concentrations of AgNPs (0, 10, 20, 30, 40, or 50 mg L−1) were used. Following 6 weeks of in vitro shoot regeneration, the shoot number, shoot length, leaf number, fresh weight, dry weight, chlorophyll content, total protein, proline level, and antioxidant enzyme activities of the plants were quantified. We found that 20 mg L−1 AgNPs increased the shoot number, shoot length, fresh weight, dry weight, and chlorophyll content of the plants. The maximum total protein was recorded in plants that were administered the lowest dose of AgNPs (10 mg L−1), while high concentrations of AgNPs (40 and 50 mg L−1) increased the levels of proline and the enzymes superoxide dismutase and catalase. Our results indicate that green-synthesized AgNPs may be of agricultural and medicinal interest owing to their effects on plants in vitro.

scholarly journals Efecto de bacterias promotoras de crecimiento vegetal (PGPR) asociadas a Pennisetum clandestinum en el altiplano cundiboyacense

2013 ◽  
Vol 13 (2) ◽  
pp. 189 ◽  
Author(s):  
Paola Jimena Criollo ◽  
Melissa Obando ◽  
Leonardo Sánchez M. ◽  
Ruth Bonilla
Keyword(s):  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Pennisetum Clandestinum ◽  
Root Dry Weight ◽  
Indole Compounds ◽  
Plant Growth Promoting ◽  
Biological Nitrogen ◽  
Absolute Control

<p><em>Pennisetum clandestinum </em>(kikuyo) es una pastura común en  los sistemas silvopastoriles del altiplano cundiboyacense,  con altas propiedades nutritivas. Por tanto estudios que  permitan mejorar el proceso de producción en términos  económicos y ambientales reviste gran importancia.  En este estudio se evaluó el papel de la inoculación con  bacterias promotoras de crecimiento vegetal (PGPR)  sobre el crecimiento de pasto kikuyo. Las cepas 4K y 5B  fueron identificadas mediante amplificación y análisis  del 16S rADN, como <em>Stenotrophomona s</em>sp. y <em>Pseudomona  s</em>sp., respectivamente, caracterizadas por su eficiencia  <em>in vitro </em>en la fijación biológica de nitrógeno, producción  de compuestos indólicos y solubilización de fosfatos. Se  evaluaron las cepas en condiciones de invernadero en tres  tiempos de crecimiento de la planta (70, 100 y 130 días). Se  evidenció que la cepa 4K incrementó el peso seco radicular  de la planta en 50% a los 70 y 100 días, mientras que la  cepa 5B mostró un comportamiento similar en el peso seco  aéreo y radicular con aumentos de hasta el 50% a los 130  d. El efecto más importante se presentó después de 100 d  donde los tratamientos TQ, TB1 y TB2, superaron en más  del 80% al testigo absoluto en el peso fresco de la parte  aérea. Estos resultados demostraron que la inoculación  de PGPR representa una alternativa biotecnológica para  promover el crecimiento de <em>P. clandestinum</em>, con efectos  relevantes en producción de biomasa 100 días después de  la siembra (dds).  </p><p> </p><p><strong>Effect of plant growth-promoting rhizobacteria  (PGPR) associated to <em>Pennisetum clandestinum </em>in  the altiplano cundiboyacense  </strong></p><p><em>Pennisetum clandestinum </em>(kikuyo) is a common  pasture in the altiplano cundiboyacense silvopastoral  systems, which possesses high nutritional value.  Therefore, studies to improve the production process  in both economic and environmental terms are very  important. The role of inoculation with plant growthpromoting  bacteria was evaluated on the growth of  kikuyu grass. The 4K and 5B strains were identified,  through amplification and analysis of their 16S rDNA,  as members of the <em>Stenotrophomonas </em>and <em>Pseudomonas  </em>genera, respectively. They were characterized <em>in vitro </em>for  their efficiency of biological nitrogen fixation, production  of indole compounds, and phosphate solubilization. Four  treatments were evaluated under greenhouse conditions.  Furthermore, the biomass was evaluated at different  stages of the plant (70, 100 and 130 days). The 4K strain  demonstrated a root dry weight that increased by 50% at  70 and 100 days and the 5B strain showed a statistically  significant behavior for plant and root dry weight with  an increase of 50% at 130 days. The most important  effect was presented after 100 d where treatments, TQ,  TB1 and TB2, exceeded more 80% to absolute control  in the fresh weight of the air. These results showed that  inoculation with PGPR represents a biotechnological  alternative to promote growth of <em>P. clandestinum</em>, as we  observed relevant effects on biomass production 100  days after planting.  </p>

scholarly journals Estrogen Differentially Regulates Protein Abundance in Exosomes Released From Immortalized Kisspeptin Neurons in Vitro

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A538-A538
Author(s):  
Teagan James ◽  
Patrick Everett Chappell
Keyword(s):  
Estrogen Receptor Alpha ◽  
Bone Matrix ◽  
Dependent Manner ◽  
Alizarin Red ◽  
Runx2 Expression ◽  
Skeletal Homeostasis ◽  
The Media ◽  
Osteogenic Factors

Abstract Estrogen (E2) is essential for multiple physiological effects in females, ensuring maximum reproductive fitness and maintaining skeletal homeostasis. E2 has been shown to stimulate cancellous bone formation via activation of estrogen receptor alpha (ERα), an effect widely accepted to be mediated directly at bone. A recent landmark study (Herber et al., Nat Commun 2019) demonstrated bone density increases in female mice harboring ERα-deletions specifically in arcuate Kiss-1 neurons. In this study, bone from transgenic females showed higher osteoblast functioning and increases in the expression of sp7 and runx2, positing a direct neural-bone regulatory axis altered by circulating E2 acting in brain. Our laboratory has used two immortalized Kisspeptin (Kiss1)-expressing and -secreting cell lines, KTaR-1 (representative of female arcuate Kiss-1 neurons) and KTaV-3 cells (representative of female AVPV Kiss-1 neurons) as models to explore the role of Kiss-1 in multiple physiological regulatory contexts. We recently determined that factors in the media of female ARC-derived KTaR-1 cells can affect parameters of osteoblast function in vitro, including increases in sp7 and runx2 expression, and formation of bone matrix (evaluated by Alizarin Red assay). Exposure of canine osteosarcoma cells to conditioned media from KTaR-1 cells led to increases in sp7 expression in an E2-dependent manner, and 24h E2-deprivation of these neurons stimulated secretion of osteogenic factors. In this current study, we have used LCMS-MS proteomic analysis to determine the contents of exosomes isolated from Kisspeptin neurons under varying E2 exposure conditions in vitro. Preliminary results reveal ~150-170 proteins up-regulated by E2 exposure and ~200-220 proteins downregulated by E2 exposure in exosomes of both KTaR-1 and KTaV-3 Kisspeptin neurons. Estrogen-regulated Kiss-1 exosomal proteins include several candidates involved in bone remodeling (pentraxin, osteonectin, osteoclast-stimulating factor-1) and neuronal synaptic plasticity and signaling (annexins, semaphorins, connexins). Current work is exploring the effects of exposure of purified exosomes on morphology and gene expression in immortalized GnRH neurons and osteoblasts. While further study is required, initial results suggest that exosomes may represent additional cellular communication pathways utilized by Kisspeptin neurons to elicit changes in brain and bone.

scholarly journals In vitro cultures of Salvia officinalis L. as a source of antioxidant compounds

2011 ◽  
Vol 74 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Izabela Grzegorczyk ◽  
Ireneusz Bilichowski ◽  
Elżbieta Mikiciuk-Olasik ◽  
Halina Wysokińska
Keyword(s):  
Cell Suspension ◽  
Rosmarinic Acid ◽  
Dry Weight ◽  
Suspension Cultures ◽  
In Vitro Cultures ◽  
Salvia Officinalis ◽  
Carnosic Acid ◽  
Shoot Cultures ◽  
Undifferentiated Callus

The concentrations of carnosic acid, carnosol and rosmarinic acid in different materials from differentiated (multiple shoot cultures and regenerated plants) and undifferentiated (callus and cell suspension) in vitro cultures of <em>Salvia officinalis</em> were determined by HPLC. The results suggested that diterpenoid (carnosic acid and carnosol) production is closely related to shoot differentiation. The highest diterpenoid yield (11.4 mg g<sup>-1</sup> for carnosic acid and 1.1 mg g<sup>-1</sup> for carnosol) was achieved in shoots of 10-week-old micropropagated plants. The levels were comparable to those found in shoots of naturally growing plants. Undifferentiated callus and cell suspension cultures produced only very low amounts of carnosol (ca. 0.05 mg g<sup>-1</sup> of dry weight). In contrast, content of rosmarinic acid in callus and suspension cultures as well as shoots growing in vitro and in vivo was similar and ranged between 11.2 and 18.6 mg g<sup>-1</sup> of dry weight.

scholarly journals Role of LDL receptors in the in vitro uptake and degradation of LDL in the media of rabbit thoracic aorta.

1989 ◽  
Vol 64 (5) ◽  
pp. 957-967 ◽  
Author(s):  
P A Curmi ◽  
G Renaud ◽  
L Juan ◽  
B Chiron ◽  
A Tedgui
Keyword(s):  
Thoracic Aorta ◽  
Ldl Receptors ◽  
The Media ◽  
In Vitro Uptake
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