scholarly journals Development of methods of identification and quantitative determination of active substances in semi-solid dosage forms with sapropel extracts

2021 ◽  
pp. 64-74
Author(s):  
Oksana Strus ◽  
Nataliia Polovko ◽  
Sergey Gushcha ◽  
Anna Filipska
Keyword(s):  
Wound Healing ◽  
Veterinary Medicine ◽  
Mass Fraction ◽  
Total Mass ◽  
Maximum Absorption ◽  
Hexane Extraction ◽  
Industry Standards ◽  
Chromatographic Profile ◽  
Active Substances ◽  
Identification And Quantification

For use in veterinary medicine for the treatment of the wound process and the prevention of mastitis, the composition and technology of an emulsion-based cream, conventionally called “Saprocream”, has been developed. Clinical trial of the drug “Saprocream” proved its effectiveness and safety for use in the healing of microtraumas, erosions and cracks (wounds) of the udders of cows. The aim of the research were was the standardization of the cream under the conventional name “Saprocream” for use in veterinary medicine as a wound healing agent, the development of methods of identification and quantification and their validation. Materials and methods. Test samples of emulsion cream type o / v, containing 15 % aqueous extract of sapropel (VES), 10 % oil extract of sapropel (OES), 6 % emulsifier No. 1, 1 % cetylstearyl alcohol, preservatives – 0.01 % nisin, 0.1 % euxil K 100 and purified water up to 100 g were made by phase inversion. The methods of pharmacopoeial articles of the European Pharmacopoeia of the 10th edition, SPhU 1.0, as well as industry standards were used to develop methods for identification and quantification of active pharmaceutical ingredients (API) in the developed cream and its standardization. Results. The chromatographic profile of the acetone extract from the cream coincides with the chromatographic profile of the reference solution ((PRS) β-carotene), which indicates the presence of substances of carotenoid structure. The absorption spectrum of hexane extraction from the cream in the range from 500 nm to 750 nm has a maximum absorption at a wavelength of 670±2 nm, which coincides with the maximum absorption of hexane solution OES, indicating the presence of chlorophyll. A method for quantifying the total mass fraction of humic acids (HA) has been developed and it has been established that the total mass fraction of HA in cream samples with sapropel extracts is 0.828 %. The results of the validation of the method show that there is a linear relationship between the concentration of the total mass fraction of HA and the mass of the AES sample with a correlation coefficient of 0.9981 (³ 0.9981). The developed technique is precise, because the value of the relative confidence interval is less than the critical value for the convergence of the results: D %=1.34≤1.60 and the criterion of insignificance of systematic error d=0.51 is fulfilled. Conclusions. An emulsion-based cream under the conventional name “Saprocream” has been standardized, methods for identification and quantification of active substances have been developed and validation of the developed methods for use in veterinary medicine as a wound-healing and anti-inflammatory agent has been carried out.

scholarly journals The Regenerative Potential of Substance P

2022 ◽  
Vol 23 (2) ◽  
pp. 750
Author(s):  
Patrycja Redkiewicz
Keyword(s):  
Wound Healing ◽  
Substance P ◽  
Autoimmune Diseases ◽  
Chronic Wounds ◽  
Different Types ◽  
Healing Wounds ◽  
Active Substances

Wound healing is a highly coordinated process which leads to the repair and regeneration of damaged tissue. Still, numerous diseases such as diabetes, venous insufficiencies or autoimmune diseases could disturb proper wound healing and lead to chronic and non-healing wounds, which are still a great challenge for medicine. For many years, research has been carried out on finding new therapeutics which improve the healing of chronic wounds. One of the most extensively studied active substances that has been widely tested in the treatment of different types of wounds was Substance P (SP). SP is one of the main neuropeptides released by nervous fibers in responses to injury. This review provides a thorough overview of the application of SP in different types of wound models and assesses its efficacy in wound healing.

Thermodynamic theory of solutions

2005 ◽  
Author(s):  
Boris S. Bokstein ◽  
Mikhail I. Mendelev ◽  
David J. Srolovitz
Keyword(s):  
Mass Fraction ◽  
Total Mass ◽  
Molar Fraction ◽  
Weight Fraction ◽  
Weight Percent ◽  
Thermodynamic Theory ◽  
Point Of View ◽  
Condensed Phases ◽  
Ideal Gases ◽  
Definition Of

A solution is a homogeneous mixture consisting of two or more components in which the composition can be continuously varied (within some range) with no change of phase. Solutions can be gases, liquids, or solids. We have already considered the properties of gaseous solutions (when we considered a mixture of ideal gases). In this chapter, we focus on condensed phases (i.e. liquids and solids). The composition of a solution can be described in several ways. Here are the most common: 1. The molar fraction of the ith component, xi, is the ratio of the number of moles of component i, ni, to the total number of moles of all species within the solution, n: 2. The weight fraction of the ith component, [i], is the ratio of the mass of component i, wi, to the total mass of all species within the solution, w: the weight fraction is often written as a weight percent [wt%] = 100[i]. 3. The molarity of the ith component, ci, is the number of moles of component i, ni per liter of solution, V: 4. The molality of ith component (used only for dilute solutions), mi, is the number of moles of component i, in 1 kg of solvent. There are several other definitions used to describe the composition of a solution, but we shall only refer to those described above in this text. The reason that there are so many definitions of the composition is related to how the term ‘‘concentration’’ is applied. For example, from the physical–chemical point of view, the molar fraction is the most convenient definition of the concentration since it is on an atomic basis. However, from the point of view of someone who has to prepare solutions from separate solutes and solvents, the mass fraction is the most convenient definition since it is directly related to the mass of the components, rather than the number of moles of the component. The former is easily measured, while the latter requires the additional knowledge of the molecular weight (and a trivial calculation).

scholarly journals Formation of globular clusters with multiple stellar populations from massive gas clumps in high-z gas-rich dwarf galaxies

2019 ◽  
Vol 622 ◽  
pp. A53 ◽  
Author(s):  
K. Bekki
Keyword(s):  
Mass Fraction ◽  
Total Mass ◽  
Globular Clusters ◽  
Dwarf Galaxies ◽  
Stellar System ◽  
Stellar Populations ◽  
Stellar Systems ◽  
Agb Stars ◽  
Time Step ◽  
Gas Accretion

Context. One of the currently favored scenarios for the formation of globular clusters (GCs) with multiple stellar populations is that an initial massive stellar system forms (“first generation”, FG), subsequently giving rise to gaseous ejecta which is converted into a second-generation (SG) of stars to form a GC. How such GCs with such FG and SG populations form and evolve, however, remains unclear. Aims. We therefore investigate, for the first time, the sequential formation processes of both FG and SG stars from star-forming massive gas clumps in gas-rich dwarf disk galaxies. Methods. We adopt a novel approach to resolve the two-stage formation of GCs in hydrodynamical simulations of dwarf galaxies. In the new simulations, new gas particles that are much less massive than their parent star particle are generated around each new star particle when the new star enters into the asymptotic giant branch (AGB) phase. Furthermore, much finer maximum time step width (~105 yr) and smaller softening length (~2 pc) are adopted for such AGB gas particles to properly resolve the ejection of gas from AGB stars and AGB feedback effects. Therefore, secondary star formation from AGB ejecta can be properly investigated in galaxy-scale simulations. Results. An FG stellar system can first form from a massive gas clump developing due to gravitational instability within its host gas-rich dwarf galaxy. Initially the FG stellar system is not a single massive cluster, but instead is composed of several irregular stellar clumps (or filaments) with a total mass larger than 106 M⊙. While the FG system is dynamically relaxing, gaseous ejecta from AGB stars can be gravitationally trapped by the FG system and subsequently converted into new stars to form a compact SG stellar system within the FG system. Interestingly, about 40% of AGB ejecta is from stars that do not belong to the FG system (“external gas accretion”). FG and SG stellar systems have different amplitudes of internal rotation and V∕σ. The mass-density (MSG−ρSG) relation for SG stellar systems can be approximated as ρSG ∝ MSG1.5. There can be a threshold total mass of GC host galaxies (Mth = [5 − 23] × 109 M⊙) beyond which the formation of GCs with compact SG stellar systems is possible. Both the initial baryonic mass fraction and the gas mass fraction in dwarfs are crucial parameters that determine whether or not GCs can contain multiple stellar populations. GCs with compact SG stellar systems are more likely to form in dwarf disks with larger gas mass fractions and higher surface mass densities. Formation of binary GCs with SGs and the subsequent GC merging are clearly seen in some models. The derived external gas-accretion process in FG systems initially consisting of stellar clumps will need to be investigated further in more sophisticated simulations.

Application of a differential technique in inductively coupled plasma emission spectrometry: presentation of a relative reference measurement procedure for the determination of total mass fraction of uranium in mineralised rocks and similar matrices

2014 ◽  
Vol 29 (10) ◽  
pp. 1912-1917 ◽  
Author(s):  
P. Murugesan ◽  
C. R. Khorge ◽  
A. A. Patwardhan ◽  
Manjeet Kumar ◽  
P. K. Tarafder ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Mass Fraction ◽  
Total Mass ◽  
Measurement Procedure ◽  
Emission Spectrometry ◽  
Plasma Emission ◽  
Reference Measurement ◽  
Inductively Coupled ◽  
Plasma Emission Spectrometry

A differential inductively coupled plasma emission spectrometry as a relative reference measurement procedure for the determination of total mass fraction of uranium in mineralized rocks, ores and similar matrices is presented.

scholarly journals Quality control of agricultural products (corn grain and sunflower seeds) criterion for the content of pesticide residues

2016 ◽  
pp. 278-283
Author(s):  
L.N. Chervyakova ◽  
T.P. Panchenko ◽  
N.M. Adamenko
Keyword(s):  
Quality Control ◽  
Mass Fraction ◽  
Phosphorous Acid ◽  
Agricultural Products ◽  
Photometric Method ◽  
Sunflower Seeds ◽  
Method Of Determination ◽  
Active Substances ◽  
Corn Grain

The method of determination of phosphorous acid and aluminum phosphite photometric method which allows to evaluate the mass fraction of active substances in the grain of corn and sunflower seeds, and gives the ability to control their content at the level of hygienic standards.

scholarly journals The Use of BrownAlgae Laminaria Saccharinain Iodine Enriched Products Aimed at PreventingIodine Deficiency

2022 ◽  
Author(s):  
Yulia Shokina ◽  
Yulia Kuchina ◽  
Ksenya Savkina ◽  
Elena Novozhilova ◽  
Katya Tatcienko ◽  
...  
Keyword(s):  
Brown Algae ◽  
Dairy Products ◽  
Mass Fraction ◽  
Total Mass ◽  
Raw Material ◽  
Optimal Dosage ◽  
Food Ingredient ◽  
Laminaria Saccharina ◽  
Fermented Dairy Products ◽  
Fermented Dairy

The high content of iodine in dried algae has allowed it to be used as a physiologically functional food ingredient in the technology of fortified foods, which can help prevent iodine deficiency. The aim of this research was to develop a technology for food products enriched with iodine (Italian crispbreads), as well as fermented dairy products (thick and drinking yoghurts with various fruit fillings). The brown algae Laminaria saccharina, which originates from the islandFrøya (in Norway), was used to enrich the products with iodine. This algaewas grown on the farms of the company ”Energy Solutions AS” and was dried by various methods of low-temperature drying (heat pump at a temperature of minus 10 ∘C, drying in 1-3 layers; vacuum freeze drying with pre-storage at a temperature of minus 8 ∘C; and pre-freezing and storing at a temperature of minus 25 ∘C for 2-6 days). The chemical composition of the algae was studied. The findings showed that the modes of pretreatment of algae and the method of cold drying did not influence the following indicators (in % of the total mass of dried algae): the mass fraction of water (6.00±0.50% to 6.93±0.50%),ash (46.40-50.14%), andsodium chloride (31.00±1.75%). The above technological factors substantially affected the content of protein and iodine in the dried algae. Thus, the mass fraction of protein varied from 5.88% to 12.35% per total mass, and iodine varied from 0.367% to 0.522% in terms of dry matter. The optimal dosage of dried algae for adding to raw material was calculated (which provided anabove-stated iodine content of % of the recommended level of adequate consumption in 100g of the developed products): from 66% in crispbreads to 88% in fermented dairy products. All new products provided a high level of organoleptic evaluation. Keywords: brown algae, Laminaria saccharina, iodine, functionalproduct, crispbreads, drinking yoghurts, thick yoghurts

scholarly journals Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5682
Author(s):  
Sander Bekeschus ◽  
Axel Kramer ◽  
Anke Schmidt
Keyword(s):  
Wound Healing ◽  
Veterinary Medicine ◽  
Animal Models ◽  
Chronic Wounds ◽  
Current Knowledge ◽  
Recent Decade ◽  
Plasma Therapy ◽  
Sequence Of Events ◽  
Gas Plasma

The loss of skin integrity is inevitable in life. Wound healing is a necessary sequence of events to reconstitute the body’s integrity against potentially harmful environmental agents and restore homeostasis. Attempts to improve cutaneous wound healing are therefore as old as humanity itself. Furthermore, nowadays, targeting defective wound healing is of utmost importance in an aging society with underlying diseases such as diabetes and vascular insufficiencies being on the rise. Because chronic wounds’ etiology and specific traits differ, there is widespread polypragmasia in targeting non-healing conditions. Reactive oxygen and nitrogen species (ROS/RNS) are an overarching theme accompanying wound healing and its biological stages. ROS are signaling agents generated by phagocytes to inactivate pathogens. Although ROS/RNS’s central role in the biology of wound healing has long been appreciated, it was only until the recent decade that these agents were explicitly used to target defective wound healing using gas plasma technology. Gas plasma is a physical state of matter and is a partially ionized gas operated at body temperature which generates a plethora of ROS/RNS simultaneously in a spatiotemporally controlled manner. Animal models of wound healing have been vital in driving the development of these wound healing-promoting technologies, and this review summarizes the current knowledge and identifies open ends derived from in vivo wound models under gas plasma therapy. While gas plasma-assisted wound healing in humans has become well established in Europe, veterinary medicine is an emerging field with great potential to improve the lives of suffering animals.

scholarly journals Competitive Capability of Some Typical Selected Veterinary Drug Manufacturers in Vietnam in the Context of International Economic Integration

2021 ◽  
Vol 11 (2) ◽  
pp. 151
Author(s):  
Thang Dac Pham
Keyword(s):  
International Economic ◽  
Veterinary Medicine ◽  
Economic Integration ◽  
Regional Economy ◽  
Veterinary Drug ◽  
Development Phase ◽  
Management Skills ◽  
Market Expansion ◽  
Industry Standards ◽  
The World

In the process of deeper and broader integration into the world and regional economy, Vietnamese businesses in general and veterinary drug manufacturers in particular have had advantages as well as disadvantages. Veterinary medicine manufacturers’ advantages are, such as: market expansion, access to science and technology, access to capital, improving management skills, etc. Nevertheless, it also faces many challenges and risks. During the development phase, Vietnamese veterinary medicine manufacturers have to face robust competition from regional and international competitors. Besides, meeting industry standards (such as GMP) is also a prerequisite for veterinary drug manufacturers in Vietnam to focus on implementation. This study analyzes the competitiveness of Vietnamese veterinary drug manufacturers, on that basis gives discussions, thereby proposing some recommendations in order to enhance the competitiveness of Vietnamese veterinary medicine manufacturers in the context of international economic integration.

scholarly journals Effectiveness of Ilmetin against neonatal diarrhea of piglets

2020 ◽  
Vol 27 ◽  
pp. 00090
Author(s):  
Anatoly I. Trubkin ◽  
Minsagit H. Lutfullin ◽  
Danil N. Mingaleev ◽  
Marina A. Efimova
Keyword(s):  
Veterinary Medicine ◽  
Growth And Development ◽  
Hematological Parameters ◽  
Healing Process ◽  
Herbal Preparation ◽  
Production Environment ◽  
Neonatal Diarrhea ◽  
Content Type ◽  
Suckling Piglets ◽  
Active Substances

The aim of the present work was to study the effectiveness of Ilmetin and the method of its use in neonatal diarrhea of piglets. The drug Ilmetin (patent for the invention No. 2542466) was developed at the Department of Epizootology and Parasitology, Kazan State Academy of Veterinary Medicine named after N.E. Bauman. A new herbal preparation was obtained by sublimation from elm bark, which contains tannins, flavonoids and other active substances. The influence of Ilmetin on hematological parameters, growth and development of piglets with neonatal diarrhea in a production environment was studied. The use of the Ilmetin preparation for the treatment of acute gastroenteritis of piglets contributed to an easier course of the disease, a quick healing process and ensured 100 % preservation of suckling piglets in comparison with the generally accepted treatment regimen for acute piglet gastroenteritis.

scholarly journals Effect of Nickle Nanoparticles Solution on Staphylococcus aureus Contaminated Open Wounds Healing in Mice

2020 ◽  
Vol 14 (4) ◽  
pp. 2637-2648
Author(s):  
Hala Mohammed Majeed ◽  
Siham Agmee Wadee ◽  
Waseem Ali Hasan
Keyword(s):  
Staphylococcus Aureus ◽  
Wound Healing ◽  
Antibiotic Resistance ◽  
Veterinary Medicine ◽  
Inhibitory Concentration ◽  
Bacterial Isolates ◽  
Open Wound ◽  
Molecular Changes ◽  
Common Pathogen ◽  
Molecular Studies

The bacteria have been noted as the main cause of late wound healing. The greatest common pathogen causing the wound contaminations is Staphylococcus aureus. The current study was carried out to isolate and diagnose the staphylococcus aureus which causes of open wound inflammation after surgery in mice process and to study the effect of nickle nanoparticles solution on bacterial isolated and evaluating the molecular and pathological techniques. The study included the collection of 60 cotton swabs from the Office of the Consultant of the Faculty of Veterinary Medicine – Tikrit University and from the external veterinary clinics (from November 2018 to March 2019) from the areas of contaminated wounds or inflamed after surgery. The results of the laboratory cultural of 60 cotton swabs used showed to isolated 50(83.3%) Staphylococcus aureus isolated. And all bacterial isolates were resistant to Doxycycline hydrochloride, Penicillin, CO-Trimoxazole, Ciprofloxacin, Cephalosporin and Penicillin. The study showed that the NFNPS used to inhibit the growth of bacterial isolated by using different concentrations the MBC killer concentration was 256μg / L and the lowest inhibitory concentration to Staphylococcus aureus was MIC 64 microgram / L). Molecular studies included the observation of the most important molecular changes at the level of DNA prior to and treatment with nanoparticles. Many variations were observed on the studied bacterial isolated Including the appearance and disappearance of DNA and its different numbers when treated with nanoparticles.As for the results of the histopathological, it was found that the injury of mice with Staphylococcus aureus antibiotic resistance emerged after about five days and the symptoms were heat, redness and swelling of the skin and the release of yellow and green purulent secretions from the place of injury. When treated mice infected with nanoparticles and antibiotics together the time of the healing was faster than the time of the healing of nanoparticles treated only.

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