scholarly journals Microbial groundwater quality and its health implications for a border-strip irrigated dairy farm catchment, South Island, New Zealand

2007 ◽  
Vol 6 (1) ◽  
pp. 83-98 ◽  
Author(s):  
Murray Close ◽  
Rod Dann ◽  
Andrew Ball ◽  
Ruth Pirie ◽  
Marion Savill ◽  
...  
Keyword(s):  
New Zealand ◽  
Shallow Groundwater ◽  
Dairy Farm ◽  
Control Group ◽  
Irrigation Season ◽  
Risk Modeling ◽  
Control Groups ◽  
E Coli ◽  
Modeling Software ◽  
Border Strip

Intensification of dairying on irrigated pastures has led to concern over the microbial quality of shallow groundwater used for drinking purposes. The effects of intensive dairying and border-strip irrigation on the leaching of E. coli and Campylobacter to shallow groundwater were assessed over a three-year period in the Waikakahi catchment, Canterbury, New Zealand. Well selection excluded other sources of contamination so that the effect of dairying with border-strip irrigation could be assessed. Groundwater samples (135) were collected, mostly during the irrigation season, with E. coli being detected in 75% of samples. Campylobacter was identified in 16 samples (12%). A risk assessment of drinking water with these levels of Campylobacter was undertaken. A probability distribution was fitted to the observed Campylobacter data and the @RISK modeling software was used, assuming a dose response relationship for Campylobacter and consumption of 1 L/day of water. The probability of infection on any given day in the study area was estimated at 0.50% to 0.76%, giving an estimated probability of infection during the irrigation season of 60% to 75%. An epidemiological assessment of the Canterbury region comparing areas encompassing dairy within major irrigation schemes (∼55% border-strip irrigation) to two control groups was undertaken. Control group 1 (CG1) encompasses areas of dairying without major irrigation schemes, and a second larger control group (CG2) comprises the rest of the Canterbury region. Comparisons of the subject group to control groups indicated that there was a statistically significant increase in age-standardised rates of campylobacteriosis (CG1 Relative Risk (RR)=1.51 (95% CI = 1.31-1.75); CG2 RR = 1.51 (1.33–1.72)); cryptosporidiosis (CG1 RR = 2.08 (1.55–2.79); CG2 RR = 5.33 (4.12–6.90)); and salmonellosis (CG2 RR = 2.05 (1.55–2.71)).

Effects of a Curriculum-Integrated Dance Program on Children’s Physical Activity

2020 ◽  
Vol 17 (11) ◽  
pp. 1179-1183
Author(s):  
Geeta Sharma ◽  
Tom Stewart ◽  
Scott Duncan
Keyword(s):  
Physical Activity ◽  
New Zealand ◽  
Control Group ◽  
Control Groups ◽  
Intervention Effects ◽  
Future Studies ◽  
School Time ◽  
And Control ◽  
The Impact ◽  
Children’S Physical Activity

Background: Curriculum-integrated dance programs are a promising but relatively under-researched strategy for increasing children’s physical activity (PA). The aim of this study was to determine the impact of a curriculum-integrated dance program on children’s PA. Methods: A total of 134 primary children aged 7–9 years from 4 New Zealand schools were assigned to either a dance group (n = 78) or a control group (n = 56). The dance group participated in a 6-week curriculum-integrated dance program during school time. Although the dance program focused on curricular learning, fitness and coordination were embedded in the dance sessions. Intensity of PA varied according to the focus of each dance session. PA was measured at baseline and postintervention using a waist-mounted ActiGraph GT3X+ accelerometer for 8 consecutive days. Results: There were no significant intervention effects on PA levels between the dance and control groups postintervention. Conclusion: Dance-embedded learning did not increase overall levels of PA in this study. Future studies may consider assessing longer term effects of a dance-based intervention, or programs that place more focus on PA promotion.

scholarly journals Reduction of Escherichia coli O157:H7 Populations in Cattle by Addition of Colicin E7-Producing E. coli to Feed

2004 ◽  
Vol 70 (10) ◽  
pp. 6053-6060 ◽  
Author(s):  
Gerry P. Schamberger ◽  
Ronald L. Phillips ◽  
Jennifer L. Jacobs ◽  
Francisco Diez-Gonzalez
Keyword(s):  
Escherichia Coli ◽  
Statistical Significance ◽  
Control Group ◽  
Control Groups ◽  
Tissue Samples ◽  
Fecal Shedding ◽  
E Coli ◽  
Colicin E7 ◽  
Maximum Decrease ◽  
And Control

ABSTRACT A cattle trial using artificially inoculated calves was conducted to determine the effect of the addition of colicinogenic Escherichia coli strains capable of producing colicin E7 (a 61-kDa DNase) to feed on the fecal shedding of serotype O157:H7. The experiment was divided into three periods. In period 1, which lasted 24 days, six calves were used as controls, and eight calves received 107 CFU of E. coli (a mixture of eight colicinogenic E. coli strains) per g of feed. Both groups were orally inoculated with nalidixic acid-resistant E. coli O157:H7 strains 7 days after the treatment started. In periods 2 and 3, the treatment and control groups were switched, and the colicinogenic E. coli dose was increased 10-fold. During period 3, which lasted as long as period 1, both groups were reinoculated with E. coli O157:H7. The numbers of E. coli O157:H7 were consistently greater in the control groups during the three periods, but comparisons within each time period determined a statistically significant (P < 0.05) difference only at day 21 of period 1. However, when the daily average counts were compared between the period 1 control group and the period 3 treatment group that included the same six animals, an overall reduction of 1.1 log10 CFU/g was observed, with a maximum decrease of 1.8 log10 CFU/g at day 21 (overall statistical significance, P = 0.001). Serotype O157:H7 was detected in 44% of the treatment group's intestinal tissue samples and in 64% of those from the control group (P < 0.04). These results indicated that the daily addition of 108 CFU of colicin E7-producing E. coli per gram of feed could reduce the fecal shedding of serotype O157:H7.

scholarly journals Use of a Nonmedicated Dietary Supplement Correlates with Increased Prevalence of Streptomycin-Sulfa-Tetracycline-Resistant Escherichia coli on a Dairy Farm

2006 ◽  
Vol 72 (7) ◽  
pp. 4583-4588 ◽  
Author(s):  
Artashes R. Khachatryan ◽  
Thomas E. Besser ◽  
Dale D. Hancock ◽  
Douglas R. Call
Keyword(s):  
Escherichia Coli ◽  
Dietary Supplement ◽  
Dairy Farm ◽  
Washington State ◽  
Growth Characteristics ◽  
Control Group ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Dry Milk ◽  
Cell Densities

ABSTRACT We examined how a dietary supplement affects the prevalence of antibiotic-resistant Escherichia coli on a dairy farm in Washington State. Between 2001 and 2004 the prevalence of fecal E. coli strains resistant to streptomycin, sulfadiazine, and tetracycline (SSuT strains) declined from 59.2% to 26.1% in the calf population. In 2003 the dairy discontinued use of a dietary supplement, and we hypothesized that the decline in prevalence of SSuT strains was related to this change in management. To test this we established three treatments in which calves received no supplement, the dietary supplement with oxytetracycline, or the dietary supplement without oxytetracycline. Calves receiving either dietary supplement had a significantly higher prevalence of SSuT E. coli than the no-supplement control group (≈37% versus 20%, respectively; P = 0.03). Importantly, there was no evidence that oxytetracycline contributed to an increased prevalence of fecal SSuT E. coli. We compared the growth characteristics of SSuT and non-SSuT E. coli in LB broth enriched with either the complete dietary supplement or its individual constituents. Both the complete dietary supplement and its vitamin D component supported a significantly higher cell density of SSuT strains (P = 0.003 and P = 0.001, respectively). The dry milk and vitamin A components of the dietary supplement did not support different cell densities. These results were consistent with selection and maintenance of SSuT E. coli due to environmental components independent of antibiotic selection.

scholarly journals EFFECTS OF ETHANOLIC EXTRACT OF RHINACANTHUS NASUTUS (L) KURZ AS AN ANTIMICROBIAL AGAINST ESCHERICHIA COLI BACTERIA USING IN VITRO METHOD

2019 ◽  
pp. 69-72
Author(s):  
SUNARTI M.BIOMED ◽  
DEBORA PANINSARI
Keyword(s):  
Escherichia Coli ◽  
Treatment Group ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Control Group ◽  
Control Groups ◽  
In Vitro Method ◽  
E Coli ◽  
Escherichia Coli Bacteria

Objective: The objective of this study was to discover of the ethanolic extract of Rhinacanthus nasutus (L) Kurz in inhibiting Escherichia coli bacteria using an in vitro method. Methods: This is an experimental study using a laboratory test with Kirby-Bauer or paper disc method by observing and measuring the inhibition zone of the ethanolic extract of R. nasutus against E. coli bacteria with extract concentrations of 15%, 30%, and 60% consisting of control groups and treatment group. The positive control group used chloramphenicol antibiotics and negative control groups used Aquadest. E. coli was incubated at 37°C for 24 h. Then, the plates were incubated for 24 h at 37°C and the diameter of the inhibition zone was observed until the 3rd day with three repetitions. Results: The results of the study showed that the mean inhibition zone of E. coli bacteria was 10.93 mm, 12.09 mm, and 18.90 mm. The results of the Shapiro–Wilk test were p=0.199. The results of the one-way analysis of variance test were p<0.05 and that of the post hoc test indicated a significant value of p<0.05. Based on the results of the research, there were significant differences in the inhibition zone between the control group and the treatment group at a concentration of 15%, 30%, and 60%. Conclusion: R. nasutus extract was effective to inhibit the growth of E. coli bacteria at concentrations of 15%, 30%, and 60%, so R. nasutus is effective as an antimicrobial.

Dairy farm wastewater treatment by an advanced pond system

2003 ◽  
Vol 48 (2) ◽  
pp. 291-297 ◽  
Author(s):  
R.J. Craggs ◽  
C.C. Tanner ◽  
J.P.S. Sukias ◽  
R.J. Davies-Colley
Keyword(s):  
New Zealand ◽  
Dairy Farm ◽  
High Rate ◽  
Great Promise ◽  
E Coli ◽  
Effluent Quality ◽  
Waste Stabilisation Ponds ◽  
Faecal Bacteria ◽  
Period Performance ◽  
Farm Wastewater

Waste stabilisation ponds (WSPs) have been used for the treatment of dairy farm wastewater in New Zealand since the 1970s. The conventional two pond WSP systems provide efficient removal of wastewater BOD5 and total suspended solids, but effluent concentrations of other pollutants including nutrients and faecal bacteria are now considered unsuitable for discharge to waterways. Advanced Pond Systems (APS) provide a potential solution. A pilot dairy farm APS consisting of an Anaerobic pond (the first pond of the conventional WSP system) followed by three ponds: a High Rate Pond (HRP), an Algae Settling Pond (ASP) and a Maturation Pond (which all replace the conventional WSP system facultative pond) was evaluated over a two year period. Performance was compared to that of the existing conventional dairy farm WSP system. APS system effluent quality was considerably higher than that of the conventional WSP system with respective median effluent concentrations of BOD5: 34 and 108 g m-3, TSS: 64 and 220 g m-3, NH4-N: 8 and 29 g m-3, DRP: 13 and 17 g m-3, and E. coli: 146 and 16195 MPN/100 ml. APS systems show great promise for upgrading conventional dairy farm WSPs in New Zealand.

scholarly journals Effects of feeding probiotic Lactobacillus spp. on growth performances, scours and Escherichia coli shedding in dairy calves

2020 ◽  
pp. 89-102
Author(s):  
SM Amanullah ◽  
R Bateen ◽  
KS Huque ◽  
MR Alam ◽  
MS Alam ◽  
...  
Keyword(s):  
Live Weight ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Control Group ◽  
Feed Conversion ◽  
Control Groups ◽  
Probiotic Group ◽  
E Coli ◽  
Growth Performances ◽  
And Control

A feeding trial was conducted on 21 crossbred (Local×Friesian, Local×Sahiwal, Sahiwal×Friesian) calves of around one week age for a period of 70 days with the objective of evaluating effects of probiotic on growth performances of calves. Calves were selected and distributed equally into three groups maintaining equal sex ratio in each group, namely the farm practice (FP), control and probiotic groups. The calves were fed on milk as 12% of their live weight up to 14 days and then 10% milk of their live weight up to 70 days of age and wheat bran was supplied to the calves from 50th day at the rate of 250g/calf. The calves of probiotic group werefedaprobiotic mixture containing lactic acid bacteria (LAB) at 0.5 g /d. The data of feed intake and growth for all groups were recorded up to 35 days and then up to 70 days for probiotic and control groups. The calves under probiotic group were achieved 82.8% and 74.5% higher gain (P<0.05) than that of FP and control group, respectively and feed conversion ratio was observed significantly (P<0.05) better in probiotic group (1.83) than that in FP (3.41) and the control group (3.50) up to 35 days of trial. However, no significant differences were found in average live weight gain and feed conversion ratio between probiotic and the control groups (1.99 vs 2.07, respectively) up to 70 days of the trial. Average counts of E. coli were lower in probiotic group than that in the control and FP. The incidence of diarrhoea in probiotic fed group was found 3 and 13 times lower compared to FP and the control group, respectively. Bangladesh J. of Livestock Res. 21-25: 89-102, 2018

scholarly journals ​Genital Changes, Haemato-biochemical Profile and Therapeutic Management of Endometritis in Crossbred Dairy Cows

2022 ◽  
Author(s):  
L.J. Dutta ◽  
R. Deka ◽  
K.C. Nath ◽  
M. Bhuyan ◽  
M. Baruti ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Treatment Protocol ◽  
Supportive Therapy ◽  
Dairy Farm ◽  
Control Group ◽  
Biochemical Profile ◽  
E Coli ◽  
Lugol’S Iodine ◽  
Service Conception ◽  
White Side

Background: Reproductive performance is one of the major determinants for the economic improvement of a dairy farm. Endometritis and other uterine complications cause decrease the pregnancy rate. In this study, the genital changes and haemato-biochemical profile of crossbred dairy cows affected with endometritis was studied to evolve a suitable treatment protocol in order to improve reproductive efficiency. Methods: Crossbred cow affected with endometritis were selected based on mucopurulent vaginal discharge at estrus and tested positive for white side test. Animals were divided randomly into 8 groups with 24 animals in each group. Different therapeutic regimens fortified with supportive treatment were opted. The therapeutic regimens were supportive therapy having bypass fat, vitamins and minerals, Lugol’s iodine, Lugol’s iodine with supportive therapy, intrauterine (IU) antibiotic, IU antibiotic with supportive therapy, E. coli. LPS, E. coli. LPS with supportive therapy and control group respectively. Efficacy of each treatment regimen was based on first service conception rate (FSCR). Result: Of all the therapeutic regimens, fortification of Lugol’s iodine with supportive therapy resulted in higher FSCR (83.33%) indicating better applicability as a treatment tool for endometritis in crossbred cows.

Conjugated Linoleic Acid Causes a Marked Increase in Liver α-Tocopherol and Liver α-Tocopherol Transfer Protein in C57BL/6 J Mice

2010 ◽  
Vol 80 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Pei-Min Chao ◽  
Wan-Hsuan Chen ◽  
Chun-Huei Liao ◽  
Huey-Mei Shaw
Keyword(s):  
Antioxidant Enzymes ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substances ◽  
Control Groups ◽  
Protein Levels ◽  
Transfer Protein ◽  
And Control

Conjugated linoleic acid (CLA) is a collective term for the positional and geometric isomers of a conjugated diene of linoleic acid (C18:2, n-6). The aims of the present study were to evaluate whether levels of hepatic α-tocopherol, α-tocopherol transfer protein (α-TTP), and antioxidant enzymes in mice were affected by a CLA-supplemented diet. C57BL/6 J mice were divided into the CLA and control groups, which were fed, respectively, a 5 % fat diet with or without 1 g/100 g of CLA (1:1 mixture of cis-9, trans-11 and trans-10, cis-12) for four weeks. α-Tocopherol levels in plasma and liver were significantly higher in the CLA group than in the control group. Liver α-TTP levels were also significantly increased in the CLA group, the α-TTP/β-actin ratio being 2.5-fold higher than that in control mice (p<0.01). Thiobarbituric acid-reactive substances were significantly decreased in the CLA group (p<0.01). There were no significant differences between the two groups in levels of three antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). The accumulation of liver α-tocopherol seen with the CLA diet can be attributed to the antioxidant potential of CLA and the ability of α-TTP induction. The lack of changes in antioxidant enzyme protein levels and the reduced lipid peroxidation in the liver of CLA mice are due to α-tocopherol accumulation.

Sri Lankan Cleft Lip and Palate Study Model Analysis: Clefts of the Secondary Palate

1993 ◽  
Vol 30 (2) ◽  
pp. 227-230 ◽  
Author(s):  
Andrew Mccance ◽  
David Roberts-Harry ◽  
Martyn Sherriff ◽  
Michael Mars ◽  
William J.B. Houston
Keyword(s):  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Model Analysis ◽  
Control Group ◽  
Tooth Size ◽  
Sri Lankan ◽  
Control Groups ◽  
Secondary Palate ◽  
Comparable Control ◽  
And Control

The study models of a group of adult Sri Lankan patients with clefts of the secondary palate were investigated. Tooth-size and arch-dimension comparisons were made with a comparable control group. Significant differences were found between the cleft and control groups in tooth sizes, chord lengths, and arch widths. The cleft group dimensions were generally smaller than those of the control group. Overjets were larger in the cleft group.

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