Microbiological investigations of two thermal baths in Budapest, Hungary. Report: effect of bathing and pool operation type on water quality

Abstract In Hungary, which is famous for its thermal baths, according to the regulations, waters are investigated in hygienic aspects with standard cultivation methods. In the present study, two thermal baths were investigated (the well and three different pool waters in both) using cultivation methods, taxon-specific polymerase chain reactions (PCRs), multiplex PCRs and next-generation amplicon sequencing. Mainly members of the natural microbial community of the well waters and bacteria originating from the environment were detected but several opportunistic pathogenic taxa, e.g., Pseudomonas aeruginosa, P. stutzeri, Acinetobacter johnsoni, Acinetobacter baumanni, Moraxella osloensis, Microbacterium paraoxydans, Legionella spp., Stenotrophomonas maltophilia and Staphylococcus aureus were revealed by the applied methods. Pools with charging-unloading operation had higher microscopic cell counts, colony-forming unit (CFU) counts, number of cocci, P. aeruginosa and S. aureus compared to the recirculation systems. Bacteria originating from human sources (e.g., skin) were identified in the pool waters with less than 1% relative abundance, and their presence was sporadic in the pools. Comparing the microbiological quality of the pools based on the first sampling time and the following four months' period it was revealed that recirculation operation type has better water quality than the charging-unloading pool operation from a hygienic point of view.

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Cattle keeping, fertilization and water quality - a pilot study

Water Science & Technology ◽

10.2166/wst.1994.0490 ◽

1994 ◽

Vol 30 (9) ◽

pp. 249-253

Author(s):

Pascal Maret

Keyword(s):

Water Management ◽

Water Quality ◽

Surface Water ◽

Pilot Study ◽

Global Approach ◽

The West ◽

Breeding Management ◽

Significant Damage ◽

Cultivation Methods ◽

Cattle Husbandry

Significant damage to surface water is caused by cattle husbandry. Only one global approach of water management and farming techniques (breeding management and cultivation methods) will ensure significant results. A pilot study run in the west of France proved that water quality can easily be improved by renovating the cattle buildings. It also showed that manure used as a fertilizer covers cultivation needs, which allows substantial savings for the farmers.

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The relationship between health effects in triathletes and microbiological quality of freshwater

Water Science & Technology ◽

10.2166/wst.1995.0549 ◽

1995 ◽

Vol 31 (5-6) ◽

pp. 19-26 ◽

Cited By ~ 7

Author(s):

G. J. Medema ◽

I. A. van Asperen ◽

J. M. Klokman-Houweling ◽

A. Nooitgedagt ◽

M. J. W. van de Laar ◽

...

Keyword(s):

Water Quality ◽

Geometric Mean ◽

Microbiological Quality ◽

Quality Parameters ◽

Health Complaints ◽

E Coli ◽

Thermotolerant Coliforms ◽

Microbiological Water Quality ◽

The Relationship ◽

Faecal Streptococci

This pilot study was carried out to determine the relationship between microbiological water quality parameters and the occurrence of health complaints among triathletes. Data were collected at an Olympic distance triathlon (n=314) and a run-bike-run (n=81; controls for exposure to fresh water). At the time of the triathlon, the concentrations of Escherichia coli , thermotolerant coliforms, faecal streptococci, entero- and reoviruses, F-specific RNA phages, Salmonella, Campylobacter, Aeromonas, Plesiomonas shigelloides, Pseudomonas aeruginosa and Staphylococcus aureus were examined over the swimming course. Information on the occurrence of health complaints during the competition and in the week thereafter was collected through a written questionnaire. The results show that triathletes and run-bike-runners are comparable with respect to factors other than water exposure (age, sex, training history, physical stress, lower intestinal health complaints during the competition) that may influence the occurrence of health complaints in the week after the competition. Triathletes and run-bike-runners reported gastro-intestinal (7.7% vs 2.5%), respiratory (5.5% vs 3.7%), skin/mucosal (2.6% vs 1.2%), general (3.5% vs 1.2%) and total symptoms (14.8% vs 7.4%) in the week after the event. The health risks for triathletes for all symptom groups are not significantly higher than for run-bike-runners. The geometric mean concentration of faecal indicator bacteria is relatively low: E. coli 170/100 ml; faecal streptococci 13/100 ml, enteroviruses were present at concentrations of 0.1/l. The group of triathletes was homogeneusly and relatively intensely exposed to water; they all swam in the same body of water at the same time and 75% reported to have swallowed freshwater. It was concluded that this study design is suitable to study the relationship between health complaints and microbiological water quality. In the summers of 1993 and 1994, a study will be carried out concerning several run-bike-runs and triathlons in freshwaters of different quality.

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18S rRNA Analysis Reveals High Diversity of Phytoplankton with Emphasis on a Naked Dinoflagellate Gymnodinium sp. at the Han River (Korea)

Diversity ◽

10.3390/d13020073 ◽

2021 ◽

Vol 13 (2) ◽

pp. 73

Author(s):

Buhari Lawan Muhammad ◽

Taehee Kim ◽

Jang-Seu Ki

Keyword(s):

Water Quality ◽

Quality Management ◽

18S Rrna ◽

Water Quality Management ◽

Operational Taxonomic Unit ◽

Amplicon Sequencing ◽

Freshwater Ecosystems ◽

Reservoir Area ◽

Han River ◽

Phytoplankton Communities

Biomonitoring of phytoplankton communities in freshwater ecosystems is imperative for efficient water quality management. In the present study, we present the seasonal diversity of phytoplankton from the non-reservoir area of the Han River (Korea), assessed using the 18S rRNA amplicon sequencing. Our results uncovered a considerably high eukaryotic diversity, which was predominantly represented by phytoplankton in all the seasons (38–63%). Of these, the diatoms, Cyclostephanos tholiformis, Stephanodiscus hantzschii, and Stephanodiscus sp., were frequently detected in spring and winter. Interestingly, for the first time in the Han River, we detected a large number of operational taxonomic unit (OTU) reads belonging to the naked dinoflagellate Gymnodinium sp., which dominated in autumn (15.8%) and was observed only in that season. Molecular cloning and quantitative real-time polymerase chain reaction (PCR) confirmed the presence of Gymnodinium sp. in the samples collected in 2012 and 2019. Moreover, a comparison of the present data with our previous data from a reservoir area (Paldang Dam) revealed similar patterns of phytoplankton communities. This molecular approach revealed a prospective toxic species that was not detected through microscopy. Collectively, resolving phytoplankton communities at a level relevant for water quality management will provide a valuable reference for future studies on phytoplankton for environmental monitoring.

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Quality assessment of drinking water in the communities of São Francisco do Conde, Baiacu, Santo Amaro and Cachoeira

I South Florida Congress of Development ◽

10.47172/sfcdv2021-0012 ◽

2021 ◽

Author(s):

JOSEINA MOUTINHO TAVARES ◽

Anderson Silva de Oliveira ◽

Paulo Moutinho Andrade de Souza ◽

Walter da Silva Junior

Keyword(s):

Water Quality ◽

Drinking Water ◽

Quality Assessment ◽

Continuous Monitoring ◽

Point Of View ◽

Chlorine Content ◽

Contaminated Water ◽

Public Agencies ◽

Total Coliforms

This work aims to evaluate the quality of drinking water in the communities of Baiacu, Sao Francisco do Conde, Santo Amaro and Cachoeira. It was verified that in some places of Santo Amaro and Sao Francisco do Conde, from the microbiological point of view, it is not appropriate for consumption, because it was found that the presence of total coliforms in 50% of the samples and that the chlorine content is below the values indicated by the standards in 75% of the analyzed samples. Thus, the consumption of contaminated water can cause diseases, so it is recommended cleaning of residential reservoirs, maintenance in pipes periodically, as well as the need for continuous monitoring of water quality and the intense participation of public agencies in these actions. Through these actions and the active participation of the population, one can preserve the environment and the lives of all those who transit in the communities of the Bay of All Saints

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From the Andes to the desert: First overview of the bacterial community in the Rimac river, the main source of water for Lima, Peru

10.1101/2020.08.16.252965 ◽

2020 ◽

Author(s):

Pedro E. Romero ◽

Erika Calla-Quispe ◽

Camila Castillo-Vilcahuaman ◽

Mateo Yokoo ◽

Hammerly Lino Fuentes-Rivera ◽

...

Keyword(s):

Metropolitan Area ◽

Bacterial Communities ◽

Geographical Location ◽

Microbiological Quality ◽

Amplicon Sequencing ◽

Urban Sewage ◽

16S Rrna Amplicon Sequencing ◽

The Andes ◽

Metabolic Functions ◽

Quality Assessments

AbstractBackgroundThe Rimac river is the main source of water for Lima, Peru’s capital megacity. The river is constantly affected by different types of contamination including mine tailings in the Andes and urban sewage in the metropolitan area. We aim to produce the first characterization of bacterial communities in the Rimac river using a 16S rRNA amplicon sequencing approach which would be useful to identify bacterial diversity and potential understudied pathogens.ResultsWe report a higher diversity in bacterial communities from the Upper and, especially, Middle Rimac compared to the Lower Rimac (Metropolitan zone). Samples were generally grouped according to their geographical location. Bacterial classes Alphaproteobacteria, Bacteroidia, Campylobacteria, Fusobacteriia, and Gammaproteobacteria were the most frequent along the river. Arcobacter cryaerophilus (Campylobacteria) was the most frequent species in the Lower Rimac while Flavobacterium succinicans (Bacteroidia) and Hypnocyclicus (Fusobacteriia) were the most predominant in the Upper Rimac. Predicted metabolic functions in the microbiota include bacterial motility, quorum sensing and xenobiotics metabolism. Additional metabolomic analyses showed the presence natural flavonoids and antibiotics in the Upper Rimac, and herbicides in the Lower Rimac.ConclusionsThe dominance in the Metropolitan area of Arcobacter cryaerophilus, an emergent pathogen associated with fecal contamination and antibiotic multiresistance, but that is not usually reported in traditional microbiological quality assessments, highlights the necessity to apply next-generation sequencing tools to improve pathogen surveillance. We believe that our study will encourage the integration of omics sciences in Peru and its application on current environmental and public health issues.

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Utilization of factor analysis in waterworks engineering

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis ◽

10.11118/actaun200452020121 ◽

2004 ◽

Vol 52 (2) ◽

pp. 121-128

Author(s):

Petra Nováková

Keyword(s):

Water Quality ◽

Factor Analysis ◽

Quality Indicators ◽

Water Reservoir ◽

Statistical Processing ◽

Point Of View ◽

Water Quality Indicators ◽

Quality Of Water ◽

The Individual

The aim of the work was to elaborate and evaluate the water quality of water reservoir Vranov nad Dyjí. Fresh water was sampled in five different locations of the reservoir (three important tributaries, dam and water captation locality). Ten, the most essential water quality indicators were selected. From the point of view of water quality indicators complexity the most integrated samples were taken in the water captation locality (period 1984 – 2002). At other locations, there were missing dates from the eightieth, but their volume was sufficient for statistical processing.Correlation analyses for the individual locations and dimensions were done as so as determination coefficients for all localities during the time period of 1994 – 2002. The results demonstrate very good allocation of the water captation from the point of view of the water flow.Multiples and factor analysis was done for the period of 1984 – 2002 in the locality Jelení zátoka where the object of water captation is situated. The results of the analysis are nine factors, which influence the water quality of the reservoir. From the point of view of the importance three factors were interpreted.The analyses and results are part of my Ph.D. thesis. The results will be used for other evaluations of the water quality in the reservoir and tributaries, for activities in the catchment’s area and for proposal processing other zones of second level of protected areas.

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CALIDAD MICROBIOLÓGICA DE Ulva lactuca Y Ulva clathrata (CHLOROPHYTA) DE LA COSTA DE LA PAZ, B.C.S., MÉXICO

CICIMAR Oceánides ◽

10.37543/oceanides.v23i1-2.42 ◽

2008 ◽

Vol 23 (1-2) ◽

pp. 35

Author(s):

R. N. Águila-Ramírez ◽

C. J. Hernández-Guerrero ◽

S. Rodríguez-Astudillo ◽

R. Guerrero-Caballero

Keyword(s):

Water Quality ◽

Microbiological Quality ◽

Marine Organisms ◽

Ulva Lactuca ◽

Fecal Coliforms ◽

Human Consumption ◽

La Paz ◽

Ecological Criteria ◽

Ulva Clathrata

Microbiological quality of Ulva lactuca and Ulva clathrata (Chlorophyta) along the coastline of La Paz, B.C.S., México Total, fecal coliforms and the enterococcus group in Ulva lactuca and U. clathrata were measured in four localities of the coastline of La Paz, B.C.S, Mexico. Levels of fecal coliforms in water were below the standard of the Ecological Criteria of Water Quality (EC-WQ) which indicates a healthy La Paz inlet (below 200 MPN/100ml). Values of fecal coliforms in algae were below the NOM-031-SSA1-1993 levels determined for marine organisms destined for human consumption. These values were high only in few months. No enterococci were detected in the samples.

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Management of Postharvest Diseases of Vegetables in a Tropical and Sub-tropical Environment of the Kingdom of Eswatini

Current Journal of Applied Science and Technology ◽

10.9734/cjast/2019/v38i630465 ◽

2020 ◽

pp. 1-13

Author(s):

Diana M. Earnshaw ◽

Michael T. Masarirambi ◽

Bonginkhosi E. Dlamini ◽

Kwanele A. Nxumalo

Keyword(s):

Management Strategies ◽

Pathogenic Fungi ◽

Essential Amino Acids ◽

Point Of View ◽

Postharvest Diseases ◽

Household Level ◽

Post Harvest ◽

Side Dish ◽

Opportunistic Pathogenic ◽

And Storage

Vegetables are important in human diets as a side dish eaten either cooked or raw as in salads. They are important for their nutritional contribution as major sources of minerals, vitamins, nine essential amino acids, beneficial phytochemicals, fibre and interesting colour from an aesthetic point of view. Despite the immerse health benefits offered by vegetables there are challenges encountered in their post-harvest handling and storage up to consumption. Challenges include post-harvest losses due to diseases. Some losses occur even at household level when vegetables are not stored appropriately when stored under the sink where humidity can be high leading to an environment which promotes diseases. Diseases in the post-harvest chain are caused by bacteria and opportunistic pathogenic fungi. Post-harvest losses of vegetables are not only a threat to nutritional security but a threat to food security as well. The aim of this research study was to document major post-harvest diseases of vegetables found in the Kingdom of Eswatini and to suggest appropriate management strategies or ways of alleviating them.

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Trans-Disciplinary Pedagogy for Science and Journalism Majors Linking Water Resource Information to Communities

Indiana Journal of Earth Sciences ◽

10.14434/ijes.v3i1.30782 ◽

2021 ◽

Vol 3 ◽

Author(s):

Lee John Florea ◽

Adam J Kuban

Keyword(s):

Water Quality ◽

Learning Environment ◽

Media Studies ◽

Procedural Knowledge ◽

Scientific Data ◽

Point Of View ◽

Stem Majors ◽

Resource Information ◽

Learning Platform ◽

Project Deliverables

Water Quality Indiana is a learning platform that leverages collaborations, community partnerships, and active mentorship of transdisciplinary student cohorts. Since 2013, this platform has engaged teams of science, technology, engineering, and mathematics (STEM) and communication and media students to investigate water quality in east central Indiana (since expanded to other domestic and international locations) in an experiential problem-based learning environment. For community partners, Water Quality Indiana provides scientific data, analysis, and multimedia deliverables about water quality, and it has a successful record of finding solutions to real-world problems. From the point of view of faculty, project deliverables enhance several aspects of a faculty portfolio. For student participants, the goal is to increase metacognition, civic engagement, and confidence in processes associated with STEM and media studies, and, therefore, the transdisciplinary skills required in an increasingly competitive workforce. Assessing learning artifacts (e.g., assignment, quizzes, or other evaluative metrics) reveals a cognitive dissonance between metacognition and accuracy in declarative knowledge related to topics in water quality—student scores did not increase in posttest data despite an increased confidence in selected answers. In contrast, pretest and posttest results, synthesis reports, and focus group data suggest that confidence in procedural knowledge in both water quality and media production significantly increased by the end of the course. Students cited time constraints imposed by academic calendars and project deadlines as a limitation of the learning environment. Course data reveal differences based on academic background and gender: 1) media studies majors became more confident in their multimedia skills, while STEM majors became less confident; 2) note-taking style and detail is more organized and meticulous for female and STEM students compared to male and media studies counterparts.

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Hydrological Variability Impact on Eutrophication in a Large Romanian Border Reservoir, Stanca–Costesti

Water ◽

10.3390/w12113065 ◽

2020 ◽

Vol 12 (11) ◽

pp. 3065

Author(s):

Gabriela Elena Dumitran ◽

Liana Ioana Vuta ◽

Bogdan Popa ◽

Florica Popa

Keyword(s):

Water Quality ◽

Trophic State ◽

Multicriteria Analysis ◽

Biological Data ◽

Point Of View ◽

Temperature Changes ◽

Hydrological Variability ◽

Hydrologic Variability ◽

Research Findings

Climate change represents one of the major challenges of our century with great potential to alter water quality, and hence, find suitable solutions becomes a must. Stanca–Costesti reservoir is one of the most important in Romania and one of the most affected by the hydrologic variability. The studies regarding the trophic state of this reservoir are few, even if there are some environmental issues in its hydrological basin that could be further investigated. According to the National Administration “Apele Romane” (ANAR) yearly reports, the Stanca–Costesti reservoir is, from the trophic state point of view, an oligotrophic lake. The current research is based on chemical and some biological data collected over 10 years (ANAR) for the trophic state of the Stanca–Costesti reservoir, using the Carlson index. The research investigates the hydrological data and spans over 10 years that were classified into three categories, namely: wet year, normal year, and dry year and the influences generated by the contrasting weather (flow and temperature changes) on the trophic state of the lake. The research findings show that the trophic state of the lake is directly influenced by the hydrological variability, namely evolving to a hypertrophic status due to concentrations of nutrients. Moreover, over the years, according to ANAR data, the water quality in the reservoir alternated. Hence, at times, the quality of the water was poor, with possible negative influences on water usage. As a consequence, we proposed that the water quality be verified monthly, and this should be done by means of a more reliable method, such as a multiparameter index or multicriteria analysis.

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