The Recovery of Enteric Viruses and Coliphages from Shellfish

1986 ◽  
Vol 18 (10) ◽  
pp. 157-157
Author(s):  
K E. U. Brodisch ◽  
G. K. Idema ◽  
P. Coubrough ◽  
W O. K. Grabow
Keyword(s):  
Adverse Effect ◽  
Enteric Viruses ◽  
The Other ◽  
Distilled Water ◽  
Study Methods ◽  
High Ph ◽  
The Poor ◽  
Form Part ◽  
Virus Recovery ◽  
Recovery Procedure

Shellfish meat was seeded with polio and reoviruses, the SA11 simian rotavirus and two coliphages, VI and MS2, in order to study methods of virus recovery. An adsorption-elution-flocculation procedure had an efficiency of recovery of about 66% for poliovirus, but only 3% for reovirus, 1% for SA11 virus and 2% for the two coliphages. The poor efficiency of recovery of all viruses except poliovirus, may be due to inactivation by exposure to low and high pH levels which form part of the recovery procedure. In another procedure seeded shellfish meat was simply homogenized in distilled water and centrifuged. The supernatant was decontaminated with chloroform and directly analyzed. The efficiency of recovery of this procedure was only about 8% for poliovirus, but 44% for MS2 and 50% for VI phages. Chloroform treatment had an adverse effect on the phages, because the elimination of this step increased the efficiency of recovery to 50% for MS2 and 62% for VI. These results indicate that the phages do not adsorb tightly to the shellfish meat and can, therefore, readily be recovered. Enteric viruses, on the other hand, adsorb tightly to shellfish meat and special procedures are required to accomplish meaningful recovery. These findings suggest that different procedures should be used for the recovery of various viruses from shellfish meat.

Óscar Romero and the Politics of Sainthood

2011 ◽  
Vol 5 (3) ◽  
pp. 265-291
Author(s):  
Manuel A. Vasquez ◽  
Anna L. Peterson
Keyword(s):  
Human Rights ◽  
Civil War ◽  
El Salvador ◽  
The Other ◽  
Post Colonial ◽  
Holy See ◽  
The Poor ◽  
The Face ◽  
Crisis Of Modernity ◽  
The One

In this article, we explore the debates surrounding the proposed canonization of Archbishop Oscar Romero, an outspoken defender of human rights and the poor during the civil war in El Salvador, who was assassinated in March 1980 by paramilitary death squads while saying Mass. More specifically, we examine the tension between, on the one hand, local and popular understandings of Romero’s life and legacy and, on the other hand, transnational and institutional interpretations. We argue that the reluctance of the Vatican to advance Romero’s canonization process has to do with the need to domesticate and “privatize” his image. This depoliticization of Romero’s work and teachings is a part of a larger agenda of neo-Romanization, an attempt by the Holy See to redeploy a post-colonial and transnational Catholic regime in the face of the crisis of modernity and the advent of postmodern relativism. This redeployment is based on the control of local religious expressions, particularly those that advocate for a more participatory church, which have proliferated with contemporary globalization

Assessment of micro and macro nutrients in poultry feeds available in Dhaka city, Bangladesh.

2017 ◽  
Vol 1 ◽  
pp. 264
Author(s):  
Md Didarul Islam ◽  
Ashiqur Rahaman ◽  
Fahmida Jannat
Keyword(s):  
Heavy Metals ◽  
Adverse Effect ◽  
The Other ◽  
Nutrient Level ◽  
Dhaka City ◽  
Toxic Heavy Metals ◽  
High Concentration ◽  
Low Concentration ◽  
Feed Formulation ◽  
Macro Nutrients

This study was based on to determine the concentration of macro and micro nutrients as well as toxic and nontoxic heavy metals present in the chicken feed available in Dhaka city of Bangladesh. All macro nutrients, if present in the feed at high concentration have some adverse effect, at the same time if this nutrient present in the feed at low concentration this have some adverse effect too. So that this nutrient level should be maintained at a marginal level. On the other side toxic heavy metals if present in the feed at very low concentration those can contaminate the total environment of the ecosystem. In this study six brand samples (starter, grower, finisher and layer) which was collected from different renowned chicken feed formulation industry in Bangladesh. Those samples were prepared for analysis by wet ashing and then metals were determined by Atomic Absorption Spectroscopy. It was found that 27.7 to 68.4, 57.3 to 121.9, 0.21 to 4.1, 0.32 to 2.1, 0.11 to 1.58, 0.28 to 2.11 and 0.28 to 1.78 for zinc, iron, copper, mercury, cadmium, nickel and cobalt respectively. It was found that essential macro and micro nutrients were present in the feed in low concentration on the other side mercury was present in high concentration in the feed samples.

Enteric viruses in drinking water supplies

2002 ◽  
Vol 2 (3) ◽  
pp. 17-22
Author(s):  
A.P. Wyn-Jones ◽  
J. Watkins ◽  
C. Francis ◽  
M. Laverick ◽  
J. Sellwood
Keyword(s):  
Drinking Water ◽  
Heavy Rainfall ◽  
Liquid Culture ◽  
Plaque Assay ◽  
Enteric Viruses ◽  
Enteric Virus ◽  
The Other ◽  
Raw Water ◽  
Rt Pcr ◽  
Water Supplies

Two rural spring drinking water supplies were studied for their enteric virus levels. In one, serving about 30 dwellings, the water was chlorinated before distribution; in the other, which served a dairy and six dwellings the water was not treated. Samples of treated (40 l) and untreated (20 l) water were taken under normal and heavy rainfall conditions over a six weeks period and concentrated by adsorption/elution and organic flocculation. Infectious enterovirus in concentrates was detected in liquid culture and enumerated by plaque assay, both in BGM cells, and concentrates were also analysed by RT-PCR. Viruses were found in both raw water supplies. Rural supplies need to be analysed for viruses as well as bacterial and protozoan pathogens if the full microbial hazard is to be determined.

Modeling and risk analysis of bromate formation from ozonation of bromide-containing waters

1996 ◽  
Vol 34 (7-8) ◽  
pp. 79-85 ◽  
Author(s):  
Rengao Song ◽  
Roger Minear ◽  
Paul Westerhoff ◽  
Gary Amy
Keyword(s):  
Water Quality ◽  
Risk Analysis ◽  
Ammonia Concentration ◽  
Quality Characteristics ◽  
The Other ◽  
High Ph ◽  
Treatment Processes ◽  
Ozone Dose ◽  
Other Hand

Empirical bromate formation models were developed from batch ozonation data to simulate the effects of important water quality characteristics and treatment processes on bromate formation. Bromate formation was favored at high pH, bromide concentration, alkalinity, and ozone dose. On the other hand, increasing DOC and ammonia concentration decreased bromate formation. Validation of the bromate models demonstrated that the models accurately simulated bromate formation. Risk analysis of bromate formation was performed on 5 utilities in which ozone was used, and it was concluded that under typical ozonation conditions, the associated risk related to bromate formation could be as high as 10−3.

scholarly journals Compatibility of Bt biopesticides and adjuvants for Spodoptera frugiperda control

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Cicero Antônio Mariano dos Santos ◽  
Joacir do Nascimento ◽  
Kelly Cristina Gonçalves ◽  
Giovani Smaniotto ◽  
Leonardo de Freitas Zechin ◽  
...  
Keyword(s):  
Spodoptera Frugiperda ◽  
Economic Importance ◽  
The Other ◽  
Distilled Water ◽  
Bt Crops

AbstractSpodoptera frugiperda is a pest of economic importance for several crops with resistance reports to Bt crops and pesticides. Eco-friendly Bt biopesticides may be an alternative to chemical insecticides due to their selectivity and specificity. However, the efficacy of Bt biopesticides may be influenced by the association with other chemicals, such as adjuvants. This study evaluated the compatibility and toxicity of Bt biopesticides mixed with adjuvants for the control of S. frugiperda. The treatments included the association of Dipel SC and Dipel PM with adjuvants. Compatibility tests were used to evaluate the Bt mixture. Bt suspensions obtained from mixtures of Bt and adjuvants at 106 and 3 × 108 spores/mL−1 were used to evaluate S. frugiperda mortality and distilled water was used as the control. The addition of the adjuvant LI increased growth and sporulation, indicating compatibility with Bt biopesticides. The other adjuvants were toxic to reducing Bt growth and sporulation. Only the mixture of Bt with LI and Bt alone was effective to S. frugiperda. The addition of adjuvants to Bt biopesticide affect the Bt sporulation, growth and mortality.

scholarly journals On Professors and Poor People—A Jurisprudential Memoir

2007 ◽  
Vol 22 (2) ◽  
pp. 527-543
Author(s):  
Robert E. Rodes
Keyword(s):  
The Other ◽  
Poor People ◽  
Public Agency ◽  
The Public ◽  
The Poor ◽  
Low Degree ◽  
Preferential Option ◽  
The Subject ◽  
The Rich ◽  
Security Deposits

But let the brother of low degree glory in his high estate: and the rich, in that he is made low.—James 1:9-10I am starting this paper after looking at the latest of a series of e-mails regarding people who cannot scrape up the security deposits required by the local gas company to turn their heat back on. They keep shivering in the corners of their bedrooms or burning their houses down with defective space heaters. The public agency that is supposed to relieve the poor refuses to pay security deposits, and the private charities that pay deposits are out of money. A bill that might improve matters has passed one House of the Legislature, and is about to die in a committee of the other House. I have a card on my desk from a former student I ran into the other day. She works in the field of utility regulation, and has promised to send me more e-mails on the subject. I also have a pile of student papers on whether a lawyer can encourage a client illegally in the country to marry her boyfriend in order not to be deported.What I am trying to do with all this material is exercise a preferential option for the poor. I am working at it in a large, comfortable chair in a large, comfortable office filled with large, comfortable books, and a large—but not so comfortable—collection of loose papers. At the end of the day, I will take some of the papers home with me to my large, comfortable, and well heated house.

THE ENZYMES OF THE TRICARBOXYLIC ACID CYCLE OF PSEUDOMONAS AERUGINOSA

1956 ◽  
Vol 2 (4) ◽  
pp. 433-440 ◽  
Author(s):  
Jack J. R. Campbell ◽  
Roberts A. Smith
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Malic Acid ◽  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
The Other ◽  
Acid Cycle ◽  
High Ph ◽  
Optimum Activity ◽  
Fresh Cell ◽  
Cell Extracts

It was demonstrated that Pseudomonas aeruginosa possesses all the enzymes necessary for the oxidation of pyruvate to CO2 and water without passing through the conventional intermediates oxalosuccinate and α-ketoglutarate. These intermediates are bypassed by the action of the enzyme isocitratase which splits d-isocitrate to succinate plus glyoxylate. This reaction was shown to be readily reversible. The malic acid dehydrogenase content was low and in addition this enzyme required a high pH for optimum activity. In fresh cell extracts at pH 7.4 its activity was only 10% that of the other enzymes of the cycle. The malic and isocitric dehydrogenases were TPN specific. The organism was also shown to possess all the enzymes necessary for the operation of the conventional tricarboxylic acid cycle.

scholarly journals STUDIES ON SALT ACTION

1925 ◽  
Vol 9 (2) ◽  
pp. 123-136
Author(s):  
Howard J. Shaughnessy ◽  
Katharine I. Criswell
Keyword(s):  
Alkaline Solution ◽  
Prolonged Exposure ◽  
Sharp Decrease ◽  
Migration Velocity ◽  
The Other ◽  
Distilled Water ◽  
Solution Ph ◽  
Complete Reversal ◽  
Locke Solution ◽  
Optimum Reaction

1. The strain of Bacterium coli used in these experiments multiplies in distilled water at pH 6.0 and pH 8.0 and in Ringer-Locke solution at pH 6.0. Under all the other conditions studied the numbers decrease with the passage of time. 2. The electrophoretic charge of the cells is highest in distilled water at pH 6.0 and pH 8.0. Under all other conditions studied the velocity of migration is decreased, but the decrease is immediate and is not affected by more prolonged exposure. 3. A strongly acid solution (pH 2.0) causes a rapid death of the cells and a sharp decrease in electrophoretic charge, sometimes leading to complete reversal. 4. A strongly alkaline solution (pH 11.0) is almost as toxic as a strongly acid one, although in distilled water the organisms survive fairly well at this reaction. Electrophoretic charge, on the other hand, is only slightly reduced in such an alkaline medium. 5. In distilled water, reactions near the neutral point are about equally favorable to both viability and electrophoretic charge, pH 8.0 showing slightly greater multiplication and a slightly higher charge than pH 11.0. In the presence of salts, however, pH 8.0 is much less favorable to viability and somewhat more favorable to electrophoretic charge than is pH 6.0. 6. Sodium chloride solutions, in the concentrations studied, all proved somewhat toxic and all tended to depress electrophoretic charge. Very marked toxicity was, however, exhibited only in a concentration of .725 M strength or over and at pH 8.0, while electrophoretic migration velocity was only slightly decreased at a concentration of .0145 M strength. 7. Calcium chloride was more toxic than NaCl, showing very marked effects in .145 M strength at pH 8.0 and in 1.45 M strength at pH 6.0. It greatly depressed electrophoretic charge even in .0145 M concentration. 8. Ringer-Locke solution proved markedly stimulating to the growth of the bacteria at pH 6.0 while at pH 8.0 it was somewhat toxic, though less so than the solutions of pure salts. It depressed migration velocity at all pH values, being more effective than NaCl in this respect, but less effective than CaCl2. 9. It would appear from these experiments that a balanced salt solution (Ringer-Locke's) may be distinctly favorable to bacterial viability in water at an optimum reaction while distinctly unfavorable in a slightly more alkaline solution. 10. Finally, while there is a certain parallelism between the influence of electrolytes upon viability and upon electrophoretic charge, the parallelism is not a close one and the two effects seem on the whole to follow entirely different laws.

scholarly journals Aferição do pH de diferentes agentes clareadores indicados para o clareamento intracoronário

2018 ◽  
Author(s):  
João Felipe Besegato ◽  
Gabriela Dos Santos Ribeiro Rocha ◽  
Marlene De Sousa Amorim ◽  
Fabio Martins Salomão ◽  
Daniel Poletto ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Statistical Analysis ◽  
Multiple Comparisons ◽  
The Other ◽  
Sodium Perborate ◽  
Distilled Water ◽  
Friedman Test ◽  
Time Intervals ◽  
Ph Values ◽  
Evaluation Time

Objective: to measure pH values of bleaching agents that are indicated to intracoronal bleaching technique in different time intervals. Methods: Each group (G) received five samples (n=5): G1 – distilled water (AD); G2 – hydrogen peroxide (H2O2) 30%; G3 – sodium perborate (PbS) + AD; G4 – PbS + H2O2 30%; G5 – sodium percarbonate (PcS) + AD; and G6 – PcS + H2O2 30%. pH values were stated using a digital pHmeter, in different time intervals: immediately after handling (T0), 24 hours (T1) and 168 hours after handling (T2). The results were submitted to statistical analysis through Kruskal-Wallis and Mann Whitney tests, in this order, allowing multiple comparisons among the groups. To verify the effect of time in each group, Friedman test was applied. Results: In the evaluation of the effect of time in each group, it was observed that G2 presented acid behavior, while the other groups exhibited values close to neutrality or alkaline. Conclusions: H2O2 30% was the only agent that showed acidic behavior in every evaluation time. Meanwhile, PcS + H2O had the highest pH values.

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