Antibacterial Activity of Copper and Cobalt Amino Acids Complexes

The antibacterial properties of differently copper and cobalt amino acids complexes on agar plates was investigated in the present study. The antibacterial activity of amino acid complexes was evaluated against on three bacteria strains (Escherichia coli, Bacillus cereus, Micrococcus luteus). Generally, the amino acids complexes were mainly active against gram-positive organisms, species like Micrococcus luteus being the most susceptible strain tested. It was registered a moderate antibacterial activity against Bacillus cereus. The microorganisms Escherichia coli, which are already known to be multi-resistant to drugs, were also resistant to the amino acids complexes but also to the free salts tested. Escherichia coli were susceptible only to the CoCl2 and copper complex with phenylalanine. The complexes with leucine and histidine seem to be more active than the parent free ligand against one or more bacterial species. Moderate activity was registered in the case of complexes with methionine and phenylalanine. From the complexes tested less efficient antibacterial activity was noted in the case of complexes with lysine and valine. These results show that cobalt and copper complexes have an antibacterial activity and suggest their potential application as antibacterial agents.

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Synthesis, Antitumor and Antibacterial Studies of New Shortened Analogues of (KLAKLAK)2-NH2 and Their Conjugates Containing Unnatural Amino Acids

Molecules ◽

10.3390/molecules26040898 ◽

2021 ◽

Vol 26 (4) ◽

pp. 898

Author(s):

Sirine Jaber ◽

Ivan Iliev ◽

Tsvetelina Angelova ◽

Veronica Nemska ◽

Inna Sulikovska ◽

...

Keyword(s):

Escherichia Coli ◽

Amino Acids ◽

Unnatural Amino Acids ◽

Antibacterial Properties ◽

Unnatural Amino Acid ◽

Moderate Activity ◽

Escherichia Coli K12 ◽

Anticancer Properties ◽

The One ◽

Natural Amino Acids

(1) Background: (KLAKLAK)2 is a representative of the antimicrobial peptide group which also shows good anticancer properties. (2) Methods: Herein, we report synthesis using SPPS and characterization by HPLC/MS of a series of shortened analogues of (KLAKLAK)2. They contain single sequence KLAKLAK as C-terminal amides. In addition, substitution of some natural amino acids with unnatural β-Ala and nor-Leu is realized. In addition, these structures are conjugated with second pharmacophore with well proven anticancer properties 1,8-naphthalimide or caffeic acid. Cytotoxicity, antiproliferative effect and antimicrobial activity of newly synthesized structures were studied. (3) Results: The obtained experimental results reveal significant selective index for substances with common chemical structure KLβAKLβAK-NH2. The antibacterial properties of newly synthesized analogues at two different concentrations 10 μM and 20 μM, were tested against Gram-negative microorganisms Escherichia coli K12 407. Only two of the studied compounds KLAKLAK-NH2 and the one conjugated with second pharmacophore 1,8-naphthalimide and unnatural amino acid nor-Leu showed moderate activity against tested strains at concentration of 20 μM. (4) Conclusions: The obtained results reveal that the introducing of 1,8-naphthalimideGly- and Caf- increase the cytotoxicity and antiproliferative activity of the peptides but not their selectivity. Only two compounds KLAKLAK-NH2 and 1,8-naphthalimideGKnLAKnLAK-NH2 show moderate activity against Escherichia coli K12 at low concentration of 20 μM.

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Chemical Constituents and Antibacterial Activity of Essential Oils of Needles of Pinus Sylvestris (Scots Pine) from South West Nigeria

10.21203/rs.3.rs-635195/v1 ◽

2021 ◽

Author(s):

Kehinde A. Oyewole ◽

Omotayo O. Oyedara ◽

Shola H. Awojide ◽

Mary O. Olawade ◽

Charles O. Adetunji

Keyword(s):

Antibacterial Activity ◽

Fatty Acid ◽

Pinus Sylvestris ◽

Scots Pine ◽

Micrococcus Luteus ◽

Chemical Constituents ◽

Bacterial Species ◽

Antibacterial Properties ◽

Biological Properties ◽

Klebsiella Pneumonia

Abstract Pinus sylvestris (Scots pine) is a coniferous plant that possesses different biological properties such as antiseptic, antioxidant, antifungal, and anti-inflammatory. This study investigated the chemical constituents and in vitro antibacterial activities of essential oil (EO) of Pinus sylvestris (Scots pine) against thirteen bacterial species using Gas chromatography/mass spectrophotometry (GC/MS) and standard agar well diffusion assay. The EO exhibited excellent antibacterial activity inhibiting all the bacterial isolates tested with the diameter zone of inhibitions (Zi) ranging from 8–24 mm. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) ranged between 0.025mg/ml and 25.000mg/ml. The highest antibacterial activity was recorded against Micrococcus luteus NCIB 196 and Klebsiella pneumonia NCIB 418 (Zi = 24 mm), while the lowest activity was recorded against Proteus vulgaris (Zi = 8 mm). The GC/MS analysis revealed the presence of 30 chemical compounds, of which seven possess antibacterial properties. These include three oxygenated monoterpenes (α-terpineol, borneol, and fenchol), two sesquiterpenes (caryophyllene and δ-cadinene), one saturated fatty acid (palmitic acid) and monounsaturated fatty acid (oleic acids). Therefore, the results suggest the potential of P. sylvestris as an antimicrobial agent for incorporation in the treatment of pathogens.

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Synthesis and Antibacterial Activity of Silver Nanoparticles Against Escherichia coli and Pseudomonas sp.

International Journal of Nanoscience ◽

10.1142/s0219581x21500435 ◽

2021 ◽

Author(s):

Md. Irfanul Hoque ◽

Sultana Afrin Jahan Rima ◽

Md. Salah Uddin ◽

M. Julkarnain

Keyword(s):

Escherichia Coli ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Chemical Reduction ◽

Bacterial Species ◽

Sodium Dodecyl ◽

Antibacterial Properties ◽

Diffusion Method ◽

Bacterial Strains ◽

X Ray Diffraction

Silver nanoparticles (AgNPs) have been synthesized by chemical reduction method using ascorbic acid as reducing agent. Silver nitrate (AgNO[Formula: see text] and sodium dodecyl sulfate (SDS) have been used as precursor and stabilizer, respectively. The prepared samples were characterized by UV-visible spectroscopy, scanning electron microscopy (SEM) and X-ray diffraction (XRD). The antibacterial activity of prepared silver nanoparticles has been assessed by using the disc diffusion method against pathogenic, gram-negative bacterial strains including Escherichia coli and Pseudomonassp. To evaluate the potential antibacterial properties of AgNPs, the discs have been impregnated and dried with three different doses like 50, 100 and 150[Formula: see text][Formula: see text]l of 20[Formula: see text][Formula: see text]g/ml concentrated AgNPs solution and placed on the Petri-dishes. The antibiotic kanamycin (5[Formula: see text][Formula: see text]g) was used as control. In all the cases, a clear and distinct zone of inhibition is observed, which suggests that AgNPs can be used as effective growth inhibitors of various bacterial species and would be promising candidate for future development of antibacterial agents.

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Iso-octenidine: Promising Octenidine Analogue with Improved Solubility

Current Organic Synthesis ◽

10.2174/1570179417666201231104453 ◽

2020 ◽

Vol 17 ◽

Author(s):

Igor K. Yakuschenko ◽

Nataliya N. Pozdeeva ◽

Viktoriya A. Mumyatova ◽

Alexey A. Terentiev ◽

Svyatoslav Ya. Gadomsky

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Micrococcus Luteus ◽

Octenidine Dihydrochloride ◽

First Time

: Iso-octenidine, an isomer of octenidine dihydrochloride, was synthesized and studied for the first time. Isooctenidine was demonstrated to be 3-fold more soluble in water in comparison to original octenidine, and both substances had remarkably similar antibacterial activity (tested on Escherichia Coli and Micrococcus luteus).

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Utilization of induction furnace steel slag based iron oxide nanocomposites for antibacterial studies

SN Applied Sciences ◽

10.1007/s42452-021-04299-9 ◽

2021 ◽

Vol 3 (3) ◽

Author(s):

J. Baalamurugan ◽

V. Ganesh Kumar ◽

T. Stalin Dhas ◽

S. Taran ◽

S. Nalini ◽

...

Keyword(s):

Antibacterial Activity ◽

Iron Oxide ◽

Building Materials ◽

Induction Furnace ◽

Micrococcus Luteus ◽

Bacterial Species ◽

Steel Slag ◽

If Steel ◽

Growth Inhibitory ◽

Microplate Reader

AbstractMetals and metal oxide-based nanocomposites play a significant role over the control of microbes. In this study, antibacterial activity of iron oxide (Fe2O3) nanocomposites based on induction furnace (IF) steel slag has been carried out. IF steel slag is an industrial by-product generated from secondary steel manufacturing process and has various metal oxides which includes Al2O3 (7.89%), MnO (5.06), CaO (1.49%) and specifically Fe2O3 (14.30%) in higher content along with metalloid SiO2 (66.42). Antibacterial activity of iron oxide nanocomposites has been revealed on bacterial species such as Micrococcus luteus, Bacillus subtilis and Staphylococcus aureus. Micrococcus luteus has undergone maximum zone of inhibition (ZOI) of 12 mm for 10 mg/mL concentration of steel slag iron oxide nanocomposite. Growth inhibitory kinetics of bacterial species has been studied using ELISA microplate reader at 660 nm by varying the concentration of steel slag iron oxide nanocomposites. The results illustrate that IF steel slag is a potential material and can be utilized in building materials to increase the resistance against biodeterioration. Graphic abstract

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Synthesis of CoFe 2 O 4 /BiOI Nanocomposite: Effects on Staphylococcus aureus, Escherichia coli, Pseudomonas Aeruginosa and Bacillus Cereus

10.21203/rs.3.rs-842251/v1 ◽

2021 ◽

Author(s):

Davood Gheidari ◽

Morteza Mehrdad ◽

Saloomeh Maleki ◽

Samanesadat Hosseini

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Bacillus Cereus ◽

Antibacterial Properties ◽

Limited Area ◽

X Ray Diffraction ◽

X Ray ◽

Inhibition Concentration ◽

Resolution Mapping

Abstract With the increase of general knowledge and the advancement of science and technology, antibacterial substances were used more than antibiotics. In our current study, the antibacterial virtues of CFO/BiOI nanocomposite were investigated due to its high importance on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus cereus. MIC, MBC , Disk Diffusion and IC50 tests Cefalotin (CF), Amoxicillin (AMX), Gentamicin (GM), Trimethoprim-sulphamethoxazole (SXT) and Ceftriaxone (CRO) antibiotics in concentration 30W, 10i, 10t , 25h and 30 were used to find the antibacterial properties of the synthesized nanocomposite, respectively. For the synthesis of nanocomposites polyethylene glycol (PEG) and sulfonic acid was used as a solvent. It is noteworthy that the synthesis was performed by heat dissolution method without the presence of surfactant. Also, various techniques such as X-Ray Diffraction(XRD), Scanning Electron Microscope (SEM), High resolution mapping and Energy Dispersive X-ray spectroscopy (EDAX) have been used to determine the properties of produced nanocomposites. SEM test results showed that the formed nanoparticles were globular and their size was limited area of 22 to 34 nm. The results showed CFO / BiOI nanocomposite exhibits strong significant biological activity against Bacillus cereus. The results of MBC (Minimum Bactericidal Concentration) and MIC (Minimum Inhibition Concentration) tests for CFO/BiOI nanocomposites on bacteria were examined in the range of 0.12-0.48 mg/ml and 0.06 to 0.24 mg/ml respectively. According to the results, the minimum IC50 value was determined at a concentration of 0.061 mg/ml. On the other hand, the most resisting and susceptible bacteria in this method were Pseudomonas aeruginosa and Bacillus cereus, respectively. These findings are identical to those of a prior study on CoFe2O4 nanoparticles antibacterial properties. MBC of the nanocomposites, 50 µl from all the tubes that showed no obvious bacterial growth were distributed on BHI agar plates and incubated for 24 h at 37 ◦C. The MBC endpoint is defined as the lowest concentration which killed 98% of the bacterial population.

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ANTIBACTERIAL ACTIVITY OF CHITOSAN AND HYDROLYZED COCONUT OIL AND THEIR COMBINATION AGAINST BACILLUS CEREUS AND ESCHERICHIA COLI

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2016.v9i5.11768 ◽

2016 ◽

Vol 9 (5) ◽

pp. 69 ◽

Cited By ~ 2

Author(s):

Jansen Silalahi ◽

Petrika Situmorang ◽

Popi Patilaya ◽

Yosy Ce Silalahi

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Bacillus Cereus ◽

Coconut Oil ◽

Inhibition Zone ◽

Research Centre ◽

Virgin Coconut Oil ◽

Inhibition Zone Diameter ◽

E Coli ◽

Zone Diameter

ABSTRACTObjective: The purpose of this study was to evaluate the antibacterial activity of chitosan, hydrolyzed coconut oil and their combination againstBacillus cereus and Escherichia coli.Methods: The materials used in this study were powder of chitosan (obtained from prawn shell produced by Laboratory of Research Centre FMIPAUniversity of Sumatera Utara) and virgin coconut oil (VCO) product of Siti Nurbaya-Indonesia. VCO was partially hydrolyzed by Lipozyme TL IM(active at sn-1,3 position) and the result called hydrolyzed virgin coconut oil (HVCO). The bacteria used in this study were B. cereus and E. coli. Theantibacterial activity of chitosan in 1% acetic acid and HVCO in dimethylsulfoxide was tested by Kirby–Bauer agar diffusion method using paper discwith diameter of 6 mm.Results: The results showed that the minimum inhibitory concentration of chitosan against B. cereus and E. coli is at concentration of 0.05% withinhibition zone diameter of 6.86 mm and 7.56 mm, respectively. MIC of HVCO against B. cereus is at concentration of 0.25% with inhibition zonediameter of 6.40 mm, and against E. coli is at a concentration of 0.50% with inhibition zone diameter of 6.20 mm. The inhibition zone diameter ofchitosan 0.05% and HVCO 0.25% in combination against B. cereus is 8.33 mm which is higher than half the sum of chitosan 0.05% and HVCO 0.25%(6.63 mm). The inhibition zone diameter of chitosan 0.05% and 0.5% HVCO in combination against E. coli is 8.53 mm which is higher than half thesum of chitosan 0.05% and HVCO 0.5% (6.53 mm).Conclusion: The findings of this study indicate that chitosan is more antibacterial than HVCO, and the interaction between chitosan and HVCO incombination demonstrated to be synergistic against B. cereus and E. coli.Keywords: Antibacterial, Chitosan, Coconut oil, Combination, Bacillus cereus, Escherichia coli.®

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A New Prenylated Flavanoid with Antibacterial Activity from Propolis Collected in Egypt

Natural Product Communications ◽

10.1177/1934578x1000500111 ◽

2010 ◽

Vol 5 (1) ◽

pp. 1934578X1000500 ◽

Cited By ~ 5

Author(s):

Ashraf El-Bassuony ◽

Sameh AbouZid

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacillus Cereus ◽

Spectroscopic Methods ◽

Gram Positive ◽

Gram Negative

A novel prenylated flavanoid, isonymphaeol-D (1), together with two known compounds, isonymphaeol-B (2) and nymphaeol-B (3), were isolated from Egyptian propolis. The structures of the isolated compounds were determined by various spectroscopic methods. 1 exhibited antibacterial activity against Gram-positive (Bacillus cereus, Staphylococcus aureus) and Gram-negative strains (Serratia sp., Pseudomonos sp., Escherichia coli).

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High Resolution Melt Assays to Detect and Identify Vibrio parahaemolyticus, Bacillus cereus, Escherichia coli, and Clostridioides difficile Bacteria

Microorganisms ◽

10.3390/microorganisms8040561 ◽

2020 ◽

Vol 8 (4) ◽

pp. 561

Author(s):

Allison C. Bender ◽

Jessica A. Faulkner ◽

Katherine Tulimieri ◽

Thomas H. Boise ◽

Kelly M. Elkins

Keyword(s):

Escherichia Coli ◽

High Resolution ◽

Bacillus Cereus ◽

Vibrio Parahaemolyticus ◽

Bacterial Species ◽

Human Pathogens ◽

High Resolution Melt ◽

E Coli ◽

Clostridioides Difficile ◽

Sensitivity Specificity

Over one hundred bacterial species have been determined to comprise the human microbiota in a healthy individual. Bacteria including Escherichia coli, Bacillus cereus, Clostridioides difficile, and Vibrio parahaemolyticus are found inside of the human body and B. cereus and E. coli are also found on the skin. These bacteria can act as human pathogens upon ingestion of contaminated food or water, if they enter an open wound, or antibiotics, and environment or stress can alter the microbiome. In this study, we present new polymerase chain reaction (PCR) high-resolution melt (HRM) assays to detect and identify the above microorganisms. Amplified DNA from C. difficile, E. coli, B. cereus, and V. parahaemolyticus melted at 80.37 ± 0.45 °C, 82.15 ± 0.37 °C, 84.43 ± 0.50 °C, and 86.74 ± 0.65 °C, respectively. A triplex PCR assay was developed to simultaneously detect and identify E. coli, B. cereus, and V. parahaemolyticus, and cultured microorganisms were successfully amplified, detected, and identified. The assays demonstrated sensitivity, specificity, reproducibility, and robustness in testing.

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An Investigation on the Electrochemical Behavior and Antibacterial and Cytotoxic Activity of Nickel Trithiocyanurate Complexes

Materials ◽

10.3390/ma13071782 ◽

2020 ◽

Vol 13 (7) ◽

pp. 1782

Author(s):

Amir M. Ashrafi ◽

Pavel Kopel ◽

Lukas Richtera

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Cyclic Voltammetry ◽

Antibacterial Activity ◽

Cytotoxic Activity ◽

Cell Lines ◽

Electrochemical Behavior ◽

Antibacterial Properties ◽

Redox Behavior ◽

Electrochemical Redox

The electrochemical redox behavior of three trinuclear Ni(II) complexes [Ni3(abb)3(H2O)3(µ-ttc)](ClO4)3 (1), [Ni3(tebb)3(H2O)3(µ-ttc)](ClO4)3·H2O (2), and [Ni3(pmdien)3(µ-ttc)](ClO4)3 (3), where abb = 1-(1H-benzimidazol-2-yl)-N-(1H-benzimidazol-2-ylmethyl)methan-amine, ttcH3 = trithiocyanuric acid, tebb = 2-[2-[2-(1H-benzimidazol-2-yl)ethylsulfanyl]ethyl]-1H-benzimidazole, and pmdien = N,N,N′,N″,N″-pentamethyldiethylenetriamine is reported. Cyclic voltammetry (CV) was applied for the study of the electrochemical behavior of these compounds. The results confirmed the presence of ttc and nickel in oxidation state +2 in the synthesized complexes. Moreover, the antibacterial properties and cytotoxic activity of complex 3 was investigated. All the complexes show antibacterial activity against Staphylococcus aureus and Escherichia coli to different extents. The cytotoxic activity of complex 3 and ttcNa3 were studied on G-361, HOS, K-562, and MCF7 cancer cell lines. It was found out that complex 3 possesses the cytotoxic activity against the tested cell lines, whereas ttcNa3 did not show any cytotoxic activity.

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