Comparison of Hypoglycemic and Antioxidant Activities of the Compounds Isolated from Ficus hispida Linn. and Ficus pomifera Wall

2020 ◽  
Vol 10 (5) ◽  
pp. 621-630
Author(s):  
Ashalata Nepram ◽  
Sujata Wangkheirakpam ◽  
Warjeet S. Laitonjam
Keyword(s):  
Methanol Extract ◽  
Antioxidant Activities ◽  
Tolerance Test ◽  
Chloroform Extract ◽  
Diabetic Rats ◽  
Hypoglycemic Activity ◽  
North East India ◽  
North East ◽  
Dpph Method ◽  
Ficus Hispida

Background: Traditional knowledge has been a legacy of the past to the present. Barks of Ficus hispida Linn. and leaves of Ficus pomifera Wall. (Moraceae) have been used traditionally for the treatment of diabetes in North-east India and many other places. As many drugs have been developed from traditional plants, the authors have taken up the plants for the study of hypoglycemic activity. Objective: To investigate the hypoglycemic activities of the triterpenoids isolated from the plants and their antioxidant activities. Methods: The bioactive compounds were determined by biochemical analysis, antioxidant activity using DPPH method. Hypoglycemic activity was detected using glucose tolerance test in normal rats and alloxan induced diabetic rats with Gliclazide as standard. Results: The biochemicals and trace elements were present in appreciable amounts. Triterpenoids, (1-5), from F. pomifera and 19-hydroxyphlogacantholide (6), 3-O-[ß-D-glucopyranosyl-(1’→2’)-α- L-rhamnopyranosyl-phlogacanthoside] (7) and galanolactone (8) along with stigmasterol (9), stigmasta- 5,22-dien-7-on-3ß-ol (10), 5-(decahydro-1,1,4a-trimethyl-6-methylene-5-yl)-3-methylpent-2- enal (11), stigmasterol glucoside (12) and stigmast-4-en-3-one (13) from F. hispida Linn., respectively, were isolated. The different extracts of the barks and leaves of these plants along with the isolated compounds had antioxidant and hyploglycemic activities. Conclusions: The five triterpenoids (1-5) were isolated from the methanol extract of the leaves of F. pomifera, and compounds (6-13) were isolated from the chloroform extract of the barks of F. hispida. Methanol extract of the leaves of F. pomifera and the chloroform extract of the barks of F. hispida; compounds (1-13) isolated from these two plants reduced DPPH free radicals in a concentrationdependent manner. It was also observed that the methanol and chloroform extracts of the plants, F. pomifera and F. hispida respectively, and the compounds (1, 6 & 7) exhibited anti-diabetic properties and also caused a highly significant reduction in the blood glucose levels of normal rats.

scholarly journals Pharmacological potential of methanol extract of Anacardium occidentale stem bark on alloxan-induced diabetic rats

2018 ◽  
Vol 5 (7) ◽  
pp. 2440-2454
Author(s):  
D. A. Omoboyowa ◽  
F. O. Afolabi ◽  
T. C. Aribigbola
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Glucose Tolerance Test ◽  
Methanol Extract ◽  
Stem Bark ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Β Cells

Background: The anti-hyperglycemic potential of methanol stem bark extract of Anacardium occidentale (MSBEAO) was investigated using an alloxan-induced diabetic rat model. Alloxan administration induces the generation of free radicals which can affect antioxidant status resulting in the disruption of the β-cells of the pancreas. Therefore, this study examines the antioxidant potential of the plant extract and the ameliorating effect on the pancreas of alloxan-induced diabetic rats. Methods: Diabetes was induced by intraperitoneal injection of 150 mg/kg body weight of alloxan monohydrate. MSBEAO, at a concentration of 100 or 200 mg/kg b.w. was orally administered to alloxan-induced diabetic rats and normal rats. The hypoglycemic effect, oral glucose tolerance test, and biochemical assay of alloxan-induced diabetic rats were assayed using standard procedures. Results: Preliminary phytochemical screening of the extract revealed the presence of alkaloids, tannins, saponins, terpenoids, carbohydrates, and phenols at moderate concentrations. The lethality dose (LD50) of the plant extract was found to be equal to or less than 5000 mg/kg b.w. The hypoglycemic effect of the extract on the non-diabetic rats revealed a significant (p<0.05) decrease in the blood glucose concentration of animals administered with 1 g/kg b.w. of the extract, compared to normal control rats administered with normal saline. In the oral glucose tolerance test, the methanol extract exerted the highest response, similar to glibenclamide after 15 and 30 minutes of administration, compared to the control rats. The methanol extract yielded the highest blood glucose lowering effects after 9 days of treatment (p<0.05), compared to diabetic rats administered with normal saline and 0.3 mg/kg b.w. of glibenclamide. Administration of the extract at 200 mg/kg b.w. showed improved pancreas architecture and regeneration of the β-cells, compared with the pancreas of animals in the other groups. Conclusion: The results of this study suggest that MSBEAO is a potentially effective agent for the management of diabetes which might result from the antioxidant-generating capacity of the stem bark.

scholarly journals Phytochemical Screening, Antimicrobial and Antioxidant Activities of Sonchus Oleraceus l (Leaves) Extracts

2020 ◽  
pp. 1-4
Author(s):  
Hatim MY Hamadnalla ◽  
◽  
Mahdi Abd Elmageed Mohammed ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Salmonella Typhi ◽  
Chloroform Extract ◽  
Antioxidant Potential ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Sonchus Oleraceus

The objective of this study to examine phytochemical analysis of sonchus oleraceus l, (asteraceae) (moliata) extracts which were extracted by four solvents, petroleum ether, chloroform, methanol and distill water and to assess their potential antibacterial against four standard bacteria gram-positive and gramnegative (bacillus subtilis staphylococcus aureu, salmonella typhi, and pscudomona aeruginosa) and antioxidant activities. (moliata) extracts using cold maceration method to extract sonchus oleraceus l, (asteraceae) locally known as (moliata) extracts, where dpph assay and paper disc diffusion assay were employed to evaluate the antioxidant and antibacterial activities respectively. The results showed that tannins, triterpenes, a sterols, flavonoids and cardiac glycosides compounds were present in the sonchus oleraceus l, (asteraceae) lives (moliata) extracts. The methanol extract of leaves showed high antioxidant potential (77± 0.03 % ) and chloroform showed low antioxidant potential activity (4±0.09) compare with propyl gallate as standard (93± 0.010 %) against dpph. Chloroform extract was effective and give moderate zone of inhibition against staphylococcus aureus. Methanol extract was effective and showed moderate zone of inhibition against staphylococcus aureus, bacillus subtilis, salmonella typhi and pseudomonas aeruginosa.

scholarly journals Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Irfan Zamzani ◽  
Nita Triadisti
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Oxidation Process ◽  
Antioxidant Activities ◽  
Alternative Source ◽  
Dpph Method ◽  
Indigenous Plant ◽  
Ic50 Values

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

Common Ancestry & Genetic Diversity of Few Indigenous Chilli Land Races of North East India

2018 ◽  
Vol 7 (6) ◽  
pp. 240-247
Author(s):  
Minakshi Bhattacharjee ◽  
Jutika Boro ◽  
Swati Singha ◽  
Hitesh Boro ◽  
Narbu Gengla ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Antioxidant Properties ◽  
Phytochemical Analysis ◽  
Common Ancestry ◽  
Oxidation Reactions ◽  
Potential Health ◽  
North East India ◽  
North East ◽  
Rapd Profile ◽  
Dpph Method

Since chilies contain high quantities of bioactive potential compounds, they are expected to possess large number of pharmacological values like antioxi-dants, antifungal, antibacterial, anti-inflammatory, antiulcer, diuretics activi-ties and is being employed for the treatment of different ailments in the indigenous system of medicine. The current study aimed at comparative evaluation of antioxidant properties, phytochemicals and RAPD assay from extracts of 6 different types of chilli found in the states of Assam and Arunachal Pradesh of North East India. The plant extracts were screened for phyto-chemicals, antioxidant analysis and RAPD profile. Phytochemical analysis shows abundant presence of alkaloids, phenols, flavonoids, saponins etc. The antioxidants activities of all the aqueous extracts were evaluated using the DPPH method. The antioxidant activity (% oxidation inhibition) ranged from as high as 86.56% in Bhut Jolokia (Capsicum assamicum) extracts to as low as 18.77% in Badami Jolokia (Capsicum annum) extract. Chilli samples studied possess potential health benefits by inhibiting many oxidation reactions caused by free radicals. Genetic diversity in 6 chilli genotypes was analyzed by RAPD markers of the fourteen random primers among which 11 were polymorphic. The phylogenetic tree showed common ancestry and the divergence among the chilli species. Based on the current study findings, it may be concluded the RAPD technology can be a useful tool in explaining the genetic diversity among chilli genotypes.

scholarly journals ISOLATION AND CHARACTERIZATION OF ANTIOXIDANT COMPOUNDS FROM ETHYL ACETATE AND METHANOL EXTRACTS OF GARCINIA DAEDALANTHERA PIERRE LEAVES

2018 ◽  
Vol 10 (1) ◽  
pp. 276
Author(s):  
Anisa Puspitaningrum ◽  
Berna Elya ◽  
Katrin .
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Column Chromatography ◽  
Spectrum Analysis ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Uv Light ◽  
Antioxidant Compounds ◽  
Isolation And Characterization ◽  
Dpph Method

Objective: This study aimed to isolate and characterize the compounds responsible for the high antioxidant activities of the ethyl acetate and methanolextracts of Garcinia daedalanthera Pierre leaves.Methods: In this study, the ethyl acetate extract was obtained by column chromatography, and the methanol extract was obtained by vacuum columnchromatography. The mobile phase comprised n-hexane, ethyl acetate, and methanol with increased polarity. Antioxidant activity was examined usingthe 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The fraction with the highest antioxidant activity was purified through column chromatography,recrystallization, and preparative thin-layer chromatography. This fraction, termed the isolate of B, was identified using DPPH and AlCl3, and itsantioxidant activity was quantitatively tested.Results: From this research, 21.7 mg of the isolate of B were obtained with an IC50 of 5.82 μg/mL. Identification using an AlCl3 sprayer producedyellow phosphorescent spots under UV light. UV-Vis spectrum analysis revealed the presence of an aromatic compound and conjugated double bonds.Infrared spectrum analysis revealed the presence of −OH, C–H alkane, C=C aromatic, C=O, and C-O-C groups.Conclusion: Based on this research, 21.7 mg of the isolate of B was derived through fractionation of the methanol extract, and this isolate exhibitedantioxidant activity with an IC50 of 5.82 μg/mL. The isolate of B was considered to be a flavonoid, as it was fluorescent under UV light (366 nm) afterbeing sprayed with AlCl3 reagent.

scholarly journals Evaluation of the Hypoglycemic Activity of Methanol Extract of Icacina Trichantha Tuber in Alloxan‐Induced Diabetic Rats and Isolation of the Active Principle

2019 ◽  
Vol 33 (S1) ◽  
Author(s):  
Monday Michael Onakpa ◽  
Julius Okwoche Ode ◽  
Victor Olumayowa Kolawole ◽  
Peter Olatunde Ajagbonna ◽  
Isaac Uzoma Asuzu
Keyword(s):  
Methanol Extract ◽  
Diabetic Rats ◽  
Hypoglycemic Activity ◽  
Active Principle

scholarly journals Dr. PI POLYPHENOL CONTENT, PHYTOCHEMISTRY COMPOSITIONS AND ANTIOXIDANT ACTIVITY OF DIFFERENT EXTRACTS FROM MARINE SPONGE AAPTOS SUBERITOIDES GROWN IN NHATRANG BAY, VIETNAM

2019 ◽  
pp. 80-86
Author(s):  
DANG XUAN CUONG ◽  
VU NGOC BOI ◽  
TRAN KHAC TRI NHAN ◽  
DINH HUU DONG ◽  
THAI MINH QUANG ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Marine Sponge ◽  
Functional Food ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Chloroform Extract ◽  
Polyphenol Content ◽  
Significant Difference ◽  
Dpph Scavenging

Objective: To investigate the content and antioxidant activities of polyphenol, the correlation between polyphenol content and their antioxidant activities, and phytochemistry compositions of different extracts from marine sponge Aaptos suberitoides commonly found growing in Nhatrang bay, Vietnam orienting application into functional food and pharmacy. Methods: Evaluating the toxicity of antioxidant polyphenol powder preparing from the initial concentrated extract was by the adjusted Behrens Karber method and a correlation between polyphenol content and antioxidant activities basing on the Pearson coefficient in Excel. Separating antioxidant polyphenol content was base on solvents polarization of n-hexane, chloroform, ethanol, ethyl acetate and n-butanol which the quantification of polyphenol content and antioxidant activities, and preliminary phytochemical compositions qualitative. Results: Antioxidant polyphenol powder did not affect mice weight during the assay time of 28 d. Polyphenol content and antioxidant activities got the highest value at chloroform extract in comparison to other extracts, a significant difference (p<0.05) and strong correlation (R2>0.9). Polyphenol content (122.682 mg gallic acid equivalent ml-1), total antioxidant activity (368.183 mg ascorbic acid equivalent ml-1), reducing power activity (24.08 mg FeSO4 equivalent ml-1) and DPPH scavenging (72.48±1.54 %) were the highest values. Alkaloids, flavonoids, steroids, tannins and triterpenoids existed in initial methanol extract. Weakly polarized polyphenol content was 70.27% in comparison to initial methanol extract. Conclusion: Antioxidant polyphenol of sponge Aaptos suberitoides has the potential for application into the field of functional food and pharmaceuticals.

scholarly journals Anticariogenic potential of Potentilla fulgens extract and its chemical constituents

2017 ◽  
Vol 9 (1) ◽  
pp. 83 ◽  
Author(s):  
Alka Choudhary ◽  
Umesh Bihade ◽  
Amit Kumar Mittal ◽  
Anupam Chatterjee ◽  
Uttam Chand Banerjee ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Antibacterial Agents ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Lactobacillus Rhamnosus ◽  
Bacterial Strains ◽  
North East India ◽  
North East ◽  
Time Kill ◽  
Crude Methanol Extract

<p><em>Streptococci</em> and <em>Lactobacilli</em> are the most common bacteria causing dental caries. <em>Potentilla fuglens</em>, native of north-east India is used by tribal people to cure teeth and gum problems. Based on its ethomedical use, its anticariogenic potential was evaluated. The crude methanol extract was found to have good anticariogenic potential. Epigallocatechingallate (<strong>4</strong>) was found to the most effective inhibitor against the tested bacterial strains. Epiafzelechin (4<em>β</em>→8) epicatechin (<strong>8) </strong>also demonstrated comparatively better activity than other dimeric compounds (MIC of 1.56, 3.12 and 3.12 μg/mL against <em>Streptococcus mutans</em>, <em>Lactobacillus acidophilus </em>and <em>Lactobacillus rhamnosus</em>,<em> </em>respectively). Time-kill studies and biofilm formation inhibition assays showed molecule <strong>8</strong> to be comparable to compound <strong>4</strong> in terms of antibacterial action. The results suggest that these compounds and Potentilla extracts could be employed as natural antibacterial agents in oral health care products.</p>

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