The Crab Heparin-Like Compound Exhibits A Strong Inhibitory Effect On Infections By Dengue Virus-2
Background: - According to the World Health Organization (WHO), two fifths of the world population is at risk of infection by DENV. There are not any safe and effective vaccine established. Sulfated glycosaminoglycans such as heparin, used as anticoagulant inhibit the initial step of dengue viral replication. Recently, an isolated heparin analogue Goniopsis cruentata (cCTH) has presented low anticoagulant effect with reduced bleeding risk. Methods : - The antiviral activity of cCTH and heparin compounds against DENV-2 in Vero cell culture was determined by quantitative RT-PCR (qRT-PCR) and titration. For this, it was carried out four trials: treatment of the cells for 2 h before viral inoculation, concomitant viral inoculation treatment, treatment after viral inoculation and virucidal assay. Subsequently, the culture supernatants were collected for periods of 24, 48 and 72 h. Results: - Our results have been demonstrated that cCTH and heparin showed antiviral activity against DENV-2. Conclusion : - These data suggest that both compounds prevented viral replication in cultured Vero cells.